AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79605


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79605.t1 MTR_7g010150 100.000 120 0 0 1 120 1 120 4.69e-83 238
MS.gene79605.t1 MTR_8g467460 99.160 119 1 0 1 119 1 119 2.73e-81 234
MS.gene79605.t1 MTR_5g040570 96.667 120 4 0 1 120 1 120 2.85e-80 231
MS.gene79605.t1 MTR_7g011050 63.717 113 25 1 1 113 1 97 5.56e-44 139
MS.gene79605.t1 MTR_8g019410 47.863 117 23 2 4 119 47 126 1.49e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79605.t1 AT1G26880 90.833 120 11 0 1 120 1 120 2.57e-75 219
MS.gene79605.t1 AT1G69620 91.597 119 10 0 1 119 1 119 9.90e-75 217
MS.gene79605.t1 AT3G28900 88.333 120 14 0 1 120 1 120 4.98e-73 213
MS.gene79605.t1 AT1G26880 91.304 92 8 0 1 92 1 92 6.96e-58 174

Find 31 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACCGCCGCACTGTTAAC+AGG 0.305052 7.3:+87887040 MS.gene79605:CDS
TGGATCCTCTTTCCAGTAAC+AGG 0.314412 7.3:-87885777 None:intergenic
GGACCTAAGTGTCCTGTTAC+TGG 0.331250 7.3:+87885765 MS.gene79605:CDS
CGCACTGTTAACAGGGCTTA+TGG 0.356563 7.3:+87887048 MS.gene79605:CDS
AACACGATGCTGGTTGGATT+TGG 0.389836 7.3:-87885543 None:intergenic
TTAACAGTGCGGCGGTTCCT+AGG 0.468395 7.3:-87887036 None:intergenic
CCTTTCCCTAACAGCTCCTC+CGG 0.470814 7.3:-87887082 None:intergenic
TTTATCCGGAGGAGCTGTTA+GGG 0.501294 7.3:+87887077 MS.gene79605:CDS
TTTCCAGTAACAGGACACTT+AGG 0.509665 7.3:-87885768 None:intergenic
ACGATGCTGGTTGGATTTGG+TGG 0.532546 7.3:-87885540 None:intergenic
CATCGTGTTGTTAAGACACC+TGG 0.536949 7.3:+87885557 MS.gene79605:CDS
AGTGTCCTGTTACTGGAAAG+AGG 0.543803 7.3:+87885772 MS.gene79605:CDS
CTTAACAACACGATGCTGGT+TGG 0.545517 7.3:-87885549 None:intergenic
GTTACTGGAAAGAGGATCCA+AGG 0.571456 7.3:+87885780 MS.gene79605:CDS
ACAACTAAGAAGAGAGCTAG+TGG 0.577057 7.3:+87885744 MS.gene79605:CDS
GTGTCTTAACAACACGATGC+TGG 0.578379 7.3:-87885553 None:intergenic
TGGCATAGCTGTGTCTCCTA+CGG 0.580308 7.3:-87885520 None:intergenic
GCTCTTCAACAAGGAAAGCA+CGG 0.587996 7.3:-87887196 None:intergenic
CCGGAGGAGCTGTTAGGGAA+AGG 0.595594 7.3:+87887082 MS.gene79605:CDS
GAACCGCCGCACTGTTAACA+GGG 0.611783 7.3:+87887041 MS.gene79605:CDS
CTAGACCTCTTGTATTCAGT+AGG 0.617053 7.3:-87887009 None:intergenic
TTACTGGAAAGAGGATCCAA+GGG 0.629653 7.3:+87885781 MS.gene79605:CDS
TGAGACCTACTGAATACAAG+AGG 0.631121 7.3:+87887004 MS.gene79605:CDS
ACAAGAGGTCTAGATTGCCT+AGG 0.636078 7.3:+87887019 MS.gene79605:CDS
TATTCAGTAGGTCTCAAGTG+TGG 0.667813 7.3:-87886997 None:intergenic
AAGCCCTGTTAACAGTGCGG+CGG 0.691394 7.3:-87887044 None:intergenic
ACTGTTAACAGGGCTTATGG+AGG 0.693206 7.3:+87887051 MS.gene79605:CDS
TTCAGAGACTCACATACCGT+AGG 0.721913 7.3:+87885504 MS.gene79605:CDS
CATAAGCCCTGTTAACAGTG+CGG 0.730145 7.3:-87887047 None:intergenic
TACTGGAAAGAGGATCCAAG+GGG 0.738216 7.3:+87885782 MS.gene79605:CDS
GTATGTGAGTCTCTGAACCA+TGG 0.739024 7.3:-87885498 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAATCAATTATTTG+TGG + chr7.3:87886068-87886087 MS.gene79605:intron 10.0%
!! ATTTGAAAATTGAAAATATT+AGG - chr7.3:87886152-87886171 None:intergenic 10.0%
!!! TTTTTTAGAAATTAATATGT+TGG + chr7.3:87885826-87885845 MS.gene79605:intron 10.0%
!! TAACAAATCTTCAATAAAAA+TGG - chr7.3:87886482-87886501 None:intergenic 15.0%
!! TTTGCAATTATTAATGTTAA+TGG + chr7.3:87886033-87886052 MS.gene79605:intron 15.0%
!!! TTTGATTAATTTGTGAAAAT+GGG + chr7.3:87885686-87885705 MS.gene79605:intron 15.0%
!!! TTTTGATTAATTTGTGAAAA+TGG + chr7.3:87885685-87885704 MS.gene79605:intron 15.0%
!! GAATGAAAATTAAAAGTAGT+AGG - chr7.3:87886856-87886875 None:intergenic 20.0%
!!! TTAATACTACAATTTTGTGA+GGG - chr7.3:87886642-87886661 None:intergenic 20.0%
!!! TTAATATGTTGGTTATACAT+AGG + chr7.3:87885837-87885856 MS.gene79605:intron 20.0%
!!! TTTAATACTACAATTTTGTG+AGG - chr7.3:87886643-87886662 None:intergenic 20.0%
! AAACTATCTCTTCTGTAATA+GGG - chr7.3:87886340-87886359 None:intergenic 25.0%
! AATGTTCTTACTTCAATGTT+GGG + chr7.3:87886102-87886121 MS.gene79605:intron 25.0%
! AGATACCCTTTCTAAATTAT+TGG + chr7.3:87886662-87886681 MS.gene79605:intron 25.0%
! AGTCACCAATAATTTAGAAA+GGG - chr7.3:87886670-87886689 None:intergenic 25.0%
! ATCTATCATGTTTCAAACAT+AGG - chr7.3:87886506-87886525 None:intergenic 25.0%
! GAAAATTAAAAGTAGTAGGT+AGG - chr7.3:87886852-87886871 None:intergenic 25.0%
! GACTTGTATTATTCATCAAT+TGG + chr7.3:87885953-87885972 MS.gene79605:intron 25.0%
! GATATATGTGAGTTTATGAT+AGG + chr7.3:87885931-87885950 MS.gene79605:intron 25.0%
! GCATCATTGAAATTATACAA+TGG - chr7.3:87886755-87886774 None:intergenic 25.0%
! TGTAAAATAGGTGGAATATT+AGG + chr7.3:87885998-87886017 MS.gene79605:intron 25.0%
! TTATCATATTTGTTCTGTTC+TGG + chr7.3:87886958-87886977 MS.gene79605:intron 25.0%
! TTGAATTCATCTATGTTAGT+TGG - chr7.3:87886182-87886201 None:intergenic 25.0%
! TTGTCTGTGTTATGTAAAAT+AGG + chr7.3:87885986-87886005 MS.gene79605:intron 25.0%
AAGTGTCAAGATTATATGCA+GGG - chr7.3:87886828-87886847 None:intergenic 30.0%
CAAACTATCTCTTCTGTAAT+AGG - chr7.3:87886341-87886360 None:intergenic 30.0%
CAACACAATATTCAATCAGA+TGG - chr7.3:87886310-87886329 None:intergenic 30.0%
CAGTCACCAATAATTTAGAA+AGG - chr7.3:87886671-87886690 None:intergenic 30.0%
GAATGTTCTTACTTCAATGT+TGG + chr7.3:87886101-87886120 MS.gene79605:intron 30.0%
TCTGTGTTATGTAAAATAGG+TGG + chr7.3:87885989-87886008 MS.gene79605:intron 30.0%
! AAAATTGATGATAGCGTCAA+AGG - chr7.3:87886552-87886571 None:intergenic 30.0%
! AAAGTGTCAAGATTATATGC+AGG - chr7.3:87886829-87886848 None:intergenic 30.0%
!!! AAATGGGTTTGTGTTGTTTT+AGG + chr7.3:87885702-87885721 MS.gene79605:intron 30.0%
!!! CTATTTTTTGCTCTTCAACA+AGG - chr7.3:87887208-87887227 None:intergenic 30.0%
!!! CTTGCTTTTCTTTTGTTTTC+TGG - chr7.3:87887250-87887269 None:intergenic 30.0%
CAATTCTACAACATTGTGGA+AGG + chr7.3:87886774-87886793 MS.gene79605:intron 35.0%
GATGCAATTCTACAACATTG+TGG + chr7.3:87886770-87886789 MS.gene79605:intron 35.0%
TCATATAGATTCATACCCCT+TGG - chr7.3:87885800-87885819 None:intergenic 35.0%
TGACAAAGAAGAACATTACC+AGG - chr7.3:87885578-87885597 None:intergenic 35.0%
TTTGTTTGTACATGATGCTG+TGG + chr7.3:87885895-87885914 MS.gene79605:intron 35.0%
! CAATTTTGTGAGGGACAATA+AGG - chr7.3:87886633-87886652 None:intergenic 35.0%
!!! TGTTGTTTTAGGTGGAAAGT+TGG + chr7.3:87885713-87885732 MS.gene79605:intron 35.0%
AAACTTAGACGCTGTGTGAT+AGG - chr7.3:87886729-87886748 None:intergenic 40.0%
ACAACTAAGAAGAGAGCTAG+TGG + chr7.3:87885744-87885763 MS.gene79605:CDS 40.0%
ATAGCGTCAAAGGTCATAGT+TGG - chr7.3:87886542-87886561 None:intergenic 40.0%
CTAGACCTCTTGTATTCAGT+AGG - chr7.3:87887012-87887031 None:intergenic 40.0%
CTTCACAAACACAGCTGAAT+TGG - chr7.3:87886423-87886442 None:intergenic 40.0%
TATTCAGTAGGTCTCAAGTG+TGG - chr7.3:87887000-87887019 None:intergenic 40.0%
TGAGACCTACTGAATACAAG+AGG + chr7.3:87887004-87887023 MS.gene79605:CDS 40.0%
TTACTGGAAAGAGGATCCAA+GGG + chr7.3:87885781-87885800 MS.gene79605:CDS 40.0%
TTTCCAGTAACAGGACACTT+AGG - chr7.3:87885771-87885790 None:intergenic 40.0%
TTTGTGAGGGACAATAAGGA+CGG - chr7.3:87886629-87886648 None:intergenic 40.0%
!!! GCTTATGGAGGTGTTTTATC+CGG + chr7.3:87887063-87887082 MS.gene79605:CDS 40.0%
!!! TCATTGCTTTTTCTCCCTGA+TGG + chr7.3:87886883-87886902 MS.gene79605:intron 40.0%
!!! TGGGTTTGTGTTGTTTTAGG+TGG + chr7.3:87885705-87885724 MS.gene79605:intron 40.0%
ACAAGAGGTCTAGATTGCCT+AGG + chr7.3:87887019-87887038 MS.gene79605:CDS 45.0%
ACTGTTAACAGGGCTTATGG+AGG + chr7.3:87887051-87887070 MS.gene79605:CDS 45.0%
AGTGCATGTCATGACCATCA+GGG - chr7.3:87886900-87886919 None:intergenic 45.0%
AGTGTCCTGTTACTGGAAAG+AGG + chr7.3:87885772-87885791 MS.gene79605:CDS 45.0%
CATAAGCCCTGTTAACAGTG+CGG - chr7.3:87887050-87887069 None:intergenic 45.0%
CTTAACAACACGATGCTGGT+TGG - chr7.3:87885552-87885571 None:intergenic 45.0%
GCTCTTCAACAAGGAAAGCA+CGG - chr7.3:87887199-87887218 None:intergenic 45.0%
GTGTCTTAACAACACGATGC+TGG - chr7.3:87885556-87885575 None:intergenic 45.0%
GTTACTGGAAAGAGGATCCA+AGG + chr7.3:87885780-87885799 MS.gene79605:CDS 45.0%
TACTGGAAAGAGGATCCAAG+GGG + chr7.3:87885782-87885801 MS.gene79605:CDS 45.0%
TGGATCCTCTTTCCAGTAAC+AGG - chr7.3:87885780-87885799 None:intergenic 45.0%
TTCAGAGACTCACATACCGT+AGG + chr7.3:87885504-87885523 MS.gene79605:CDS 45.0%
TTTATCCGGAGGAGCTGTTA+GGG + chr7.3:87887077-87887096 MS.gene79605:CDS 45.0%
! AACACGATGCTGGTTGGATT+TGG - chr7.3:87885546-87885565 None:intergenic 45.0%
! CATCGTGTTGTTAAGACACC+TGG + chr7.3:87885557-87885576 MS.gene79605:CDS 45.0%
! TTTTATCCGGAGGAGCTGTT+AGG + chr7.3:87887076-87887095 MS.gene79605:CDS 45.0%
!!! TATGGAGGTGTTTTATCCGG+AGG + chr7.3:87887066-87887085 MS.gene79605:CDS 45.0%
CGCACTGTTAACAGGGCTTA+TGG + chr7.3:87887048-87887067 MS.gene79605:CDS 50.0%
GAGTGCATGTCATGACCATC+AGG - chr7.3:87886901-87886920 None:intergenic 50.0%
GGACCTAAGTGTCCTGTTAC+TGG + chr7.3:87885765-87885784 MS.gene79605:CDS 50.0%
GTCTCAAGTGTGGAATCTGC+AGG - chr7.3:87886990-87887009 None:intergenic 50.0%
TGGCATAGCTGTGTCTCCTA+CGG - chr7.3:87885523-87885542 None:intergenic 50.0%
! ACGATGCTGGTTGGATTTGG+TGG - chr7.3:87885543-87885562 None:intergenic 50.0%
AAGCCCTGTTAACAGTGCGG+CGG - chr7.3:87887047-87887066 None:intergenic 55.0%
CCTTTCCCTAACAGCTCCTC+CGG - chr7.3:87887085-87887104 None:intergenic 55.0%
GAACCGCCGCACTGTTAACA+GGG + chr7.3:87887041-87887060 MS.gene79605:CDS 55.0%
TTAACAGTGCGGCGGTTCCT+AGG - chr7.3:87887039-87887058 None:intergenic 55.0%
CCGGAGGAGCTGTTAGGGAA+AGG + chr7.3:87887082-87887101 MS.gene79605:CDS 60.0%
GGAACCGCCGCACTGTTAAC+AGG + chr7.3:87887040-87887059 MS.gene79605:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 87885500 87887283 87885500 ID=MS.gene79605
chr7.3 mRNA 87885500 87887283 87885500 ID=MS.gene79605.t1;Parent=MS.gene79605
chr7.3 exon 87885500 87885578 87885500 ID=MS.gene79605.t1.exon1;Parent=MS.gene79605.t1
chr7.3 CDS 87885500 87885578 87885500 ID=cds.MS.gene79605.t1;Parent=MS.gene79605.t1
chr7.3 exon 87885724 87885803 87885724 ID=MS.gene79605.t1.exon2;Parent=MS.gene79605.t1
chr7.3 CDS 87885724 87885803 87885724 ID=cds.MS.gene79605.t1;Parent=MS.gene79605.t1
chr7.3 exon 87886994 87887103 87886994 ID=MS.gene79605.t1.exon3;Parent=MS.gene79605.t1
chr7.3 CDS 87886994 87887103 87886994 ID=cds.MS.gene79605.t1;Parent=MS.gene79605.t1
chr7.3 exon 87887190 87887283 87887190 ID=MS.gene79605.t1.exon4;Parent=MS.gene79605.t1
chr7.3 CDS 87887190 87887283 87887190 ID=cds.MS.gene79605.t1;Parent=MS.gene79605.t1
Gene Sequence

>MS.gene79605

ATGGTTCAGAGACTCACATACCGTAGGAGACACAGCTATGCCACCAAATCCAACCAGCATCGTGTTGTTAAGACACCTGGTAATGTTCTTCTTTGTCAAATTGTTCTGTTTTTTCTTGTTCTAATCTATGTTATGATGTTTTTGATTTTTTCTTAAAAATTGCATTTTTTTGTTGTTGAAGTGTTTTTTGATTAATTTGTGAAAATGGGTTTGTGTTGTTTTAGGTGGAAAGTTGGTTTATCAGACAACTAAGAAGAGAGCTAGTGGACCTAAGTGTCCTGTTACTGGAAAGAGGATCCAAGGGGTATGAATCTATATGATATCATTTTTTTAGAAATTAATATGTTGGTTATACATAGGATCTGTGATTCATTGTGTTGATTCTATTTTTTTTGTTTGTTTGTACATGATGCTGTGGAAAAATGTAGATTGATATATGTGAGTTTATGATAGGACTTGTATTATTCATCAATTGGTTGTGTGAATTTGTCTGTGTTATGTAAAATAGGTGGAATATTAGGATTGTTTTGTGTTTTGCAATTATTAATGTTAATGGAATGCTGAGCTTATTATAAAATCAATTATTTGTGGTTTATTGAGCGAATGTTCTTACTTCAATGTTGGGTTTACTGTTTGATTGTTTGAAAATCCTAATATTTTCAATTTTCAAATCTATATTCCAACTAACATAGATGAATTCAATCAAATCATTTGTCTTGTATTTTGATTAATGTTAATCGCTGAGCATATTATGAAATCAATTACTGTGTTTTTTTTAGTTAGCTTTTACTGTCTTTTATGCATGCTCCATCTGATTGAATATTGTGTTGTTTACGTCCCTATTACAGAAGAGATAGTTTGTTTTCTGCTTTAATTATGTGATGATTCAGTTTTTTTGAAGACGTAAATAACTTAATTCACCAATTCAGCTGTGTTTGTGAAGTGAATTAGTATAGTCTGTCTGTTTTTTTTGTTAAGTCCATTTTTATTGAAGATTTGTTAGCCTATGTTTGAAACATGATAGATATAATAATCATTTCCAACTATGACCTTTGACGCTATCATCAATTTTATAATGACAAAAATTAGATAGTTAGACTTAGATGCAATTCTTTTGAGATTGACACCGTCCTTATTGTCCCTCACAAAATTGTAGTATTAAAGATACCCTTTCTAAATTATTGGTGACTGTTTTTCTTATTTAGTTACAAACAACTAGAGCAGTGCCTATCACACAGCGTCTAAGTTTTGTCCATTGTATAATTTCAATGATGCAATTCTACAACATTGTGGAAGGAACATACAATGAATGTATTACTGTGTTACCCTGCATATAATCTTGACACTTTCCTACCTACTACTTTTAATTTTCATTCAAAGATTTCATTGCTTTTTCTCCCTGATGGTCATGACATGCACTCTTTATCTTTAGTTGCAAGTTGATCAATGTGTGTTCTCTTATCATATTTGTTCTGTTCTGGACTTGACCTGCAGATTCCACACTTGAGACCTACTGAATACAAGAGGTCTAGATTGCCTAGGAACCGCCGCACTGTTAACAGGGCTTATGGAGGTGTTTTATCCGGAGGAGCTGTTAGGGAAAGGTATGTTGCTCATAACATAATTACAGATTTATATTCAAAATTTTGAATATTGTAGTAAATTTTAATGCTCTTCAATCTTTGTGCAGAATTATCCGTGCTTTCCTTGTTGAAGAGCAAAAAATAGTTAAGAAAGTTTTGAAGATCCAGAAAACAAAAGAAAAGCAAGCAGCTAAGAGTTAA

Protein sequence

>MS.gene79605.t1

MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEEQKIVKKVLKIQKTKEKQAAKS