Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80729.t1 | XP_024629315.1 | 82.6 | 470 | 74 | 4 | 1 | 470 | 1 | 462 | 3.00E-213 | 751.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80729.t1 | Q557M8 | 22.4 | 393 | 222 | 10 | 8 | 382 | 14 | 341 | 1.5e-11 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80729.t1 | A0A396IJ19 | 89.8 | 470 | 48 | 0 | 1 | 470 | 1 | 470 | 3.2e-212 | 747.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene80729 | 0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene04920 | MS.gene80729 | 0.803607 | 3.22E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene80729 | 0.802547 | 5.33E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene80729 | 0.833076 | 6.56E-56 | -1.69E-46 |
| MS.gene049807 | MS.gene80729 | 0.840407 | 8.86E-58 | -1.69E-46 |
| MS.gene050258 | MS.gene80729 | 0.810285 | 1.24E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene80729 | 0.806317 | 8.72E-50 | -1.69E-46 |
| MS.gene050646 | MS.gene80729 | 0.809473 | 1.86E-50 | -1.69E-46 |
| MS.gene051019 | MS.gene80729 | 0.802806 | 4.71E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene80729 | 0.827778 | 1.30E-54 | -1.69E-46 |
| MS.gene051279 | MS.gene80729 | 0.806952 | 6.41E-50 | -1.69E-46 |
| MS.gene051832 | MS.gene80729 | 0.829381 | 5.31E-55 | -1.69E-46 |
| MS.gene052072 | MS.gene80729 | 0.820767 | 5.76E-53 | -1.69E-46 |
| MS.gene052307 | MS.gene80729 | 0.822379 | 2.45E-53 | -1.69E-46 |
| MS.gene052518 | MS.gene80729 | 0.80556 | 1.26E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene80729 | 0.809419 | 1.91E-50 | -1.69E-46 |
| MS.gene052793 | MS.gene80729 | 0.805125 | 1.55E-49 | -1.69E-46 |
| MS.gene053272 | MS.gene80729 | -0.820316 | 7.32E-53 | -1.69E-46 |
| MS.gene054142 | MS.gene80729 | 0.815604 | 8.49E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene80729 | 0.800395 | 1.47E-48 | -1.69E-46 |
| MS.gene054709 | MS.gene80729 | 0.80144 | 8.99E-49 | -1.69E-46 |
| MS.gene055229 | MS.gene80729 | 0.816221 | 6.18E-52 | -1.69E-46 |
| MS.gene055339 | MS.gene80729 | 0.82868 | 7.86E-55 | -1.69E-46 |
| MS.gene056082 | MS.gene80729 | 0.810893 | 9.19E-51 | -1.69E-46 |
| MS.gene056363 | MS.gene80729 | 0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene80729 | 0.818658 | 1.75E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene80729 | 0.856384 | 3.34E-62 | -1.69E-46 |
| MS.gene057102 | MS.gene80729 | 0.883202 | 5.48E-71 | -1.69E-46 |
| MS.gene057132 | MS.gene80729 | 0.820112 | 8.15E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene80729 | 0.800819 | 1.21E-48 | -1.69E-46 |
| MS.gene058781 | MS.gene80729 | 0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene80729 | 0.810029 | 1.41E-50 | -1.69E-46 |
| MS.gene059778 | MS.gene80729 | 0.808957 | 2.40E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene80729 | -0.834715 | 2.55E-56 | -1.69E-46 |
| MS.gene060338 | MS.gene80729 | 0.811624 | 6.38E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene80729 | 0.827815 | 1.27E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene80729 | 0.834842 | 2.37E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene80729 | 0.824537 | 7.65E-54 | -1.69E-46 |
| MS.gene061188 | MS.gene80729 | 0.856801 | 2.52E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08242 | MS.gene80729 | PPI |
| MS.gene007951 | MS.gene80729 | PPI |
| MS.gene39443 | MS.gene80729 | PPI |
| MS.gene031871 | MS.gene80729 | PPI |
| MS.gene007950 | MS.gene80729 | PPI |
| MS.gene31944 | MS.gene80729 | PPI |
| MS.gene052488 | MS.gene80729 | PPI |
| MS.gene33782 | MS.gene80729 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80729.t1 | MTR_8g077000 | 84.394 | 487 | 51 | 6 | 1 | 470 | 1 | 479 | 0.0 | 780 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80729.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene80729.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene80729.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene80729.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene80729.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene80729.t1 | AT4G20810 | 47.548 | 469 | 185 | 16 | 8 | 469 | 1 | 415 | 1.79e-130 | 384 |
| MS.gene80729.t1 | AT4G20340 | 52.542 | 413 | 169 | 12 | 48 | 451 | 4 | 398 | 8.29e-128 | 377 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.4:-16322165 | None:intergenic |
| AACTGACGGTACATCTTTAA+AGG | 0.182546 | 8.4:+16327706 | MS.gene80729:CDS |
| GACTTGCCTGTTCCAGAATT+TGG | 0.189652 | 8.4:+16325469 | MS.gene80729:CDS |
| ATCTGCTGCGTTCGCATCTT+GGG | 0.226916 | 8.4:-16328100 | None:intergenic |
| CATCTGCTGCGTTCGCATCT+TGG | 0.249410 | 8.4:-16328101 | None:intergenic |
| TCGTCCTCGTCGTCTTCTTC+TGG | 0.291126 | 8.4:-16329977 | None:intergenic |
| GGGCTGATTATCTCCTTTGC+TGG | 0.323516 | 8.4:-16322144 | None:intergenic |
| GCTTGAAGACTACCAAATTC+TGG | 0.326095 | 8.4:-16325481 | None:intergenic |
| AAATCTCATCTGGGATATAA+TGG | 0.332016 | 8.4:+16325565 | MS.gene80729:CDS |
| GAGAAATTGGTACTTGTTGT+AGG | 0.340168 | 8.4:-16321990 | None:intergenic |
| GGAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.4:+16322152 | MS.gene80729:CDS |
| ACGACGGCGTGAAAAGTTAA+AGG | 0.370019 | 8.4:+16323981 | MS.gene80729:CDS |
| TGGTGATTCTAAATCTCATC+TGG | 0.375711 | 8.4:+16325555 | MS.gene80729:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.378747 | 8.4:+16328198 | MS.gene80729:CDS |
| GAAGCCCGAGCTAGTGCAGC+TGG | 0.384987 | 8.4:+16325508 | MS.gene80729:CDS |
| AGAGAACAAGAACACCATTC+AGG | 0.401351 | 8.4:+16323659 | MS.gene80729:CDS |
| CACGCCCAGCTGCACTAGCT+CGG | 0.403153 | 8.4:-16325513 | None:intergenic |
| GCTTAAGAAGCAACGCGAAT+TGG | 0.412214 | 8.4:+16328059 | MS.gene80729:CDS |
| TTATTTCAGATACAATGCTT+TGG | 0.415674 | 8.4:+16323822 | MS.gene80729:intron |
| GGCTGAAGTGTACCTCTAAC+AGG | 0.418995 | 8.4:-16328210 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.4:+16325523 | MS.gene80729:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.4:-16322358 | None:intergenic |
| ATTTGGTAGTCTTCAAGCTT+GGG | 0.444477 | 8.4:+16325486 | MS.gene80729:CDS |
| ACGCCCAGCTGCACTAGCTC+GGG | 0.452951 | 8.4:-16325512 | None:intergenic |
| CGGTTGGAAGTACTGCTAGA+AGG | 0.463810 | 8.4:-16328126 | None:intergenic |
| AGGAAAAGAGGGAGAAGAAA+AGG | 0.464405 | 8.4:+16323355 | MS.gene80729:CDS |
| ATAAGTTAGCTGCTCAAGAC+GGG | 0.467809 | 8.4:+16323923 | MS.gene80729:CDS |
| AGATTATCACTAGAGATCAT+AGG | 0.477747 | 8.4:+16322414 | MS.gene80729:CDS |
| AATTTGGTAGTCTTCAAGCT+TGG | 0.481156 | 8.4:+16325485 | MS.gene80729:CDS |
| TTCCACTGTGAACGTTGTAA+TGG | 0.482559 | 8.4:+16323880 | MS.gene80729:CDS |
| AGACTACCAAATTCTGGAAC+AGG | 0.482675 | 8.4:-16325475 | None:intergenic |
| AAAGGACATGCTTCAGAAGA+TGG | 0.491664 | 8.4:+16323999 | MS.gene80729:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.4:+16327734 | MS.gene80729:CDS |
| AAATGCAATTGGAGCTTACA+AGG | 0.494474 | 8.4:+16329925 | MS.gene80729:CDS |
| TGTACATATATATTCCTGAA+TGG | 0.494787 | 8.4:-16323673 | None:intergenic |
| ACAGCGTGGAGAGGTGAAGC+AGG | 0.499690 | 8.4:+16327775 | MS.gene80729:CDS |
| TATGTACAAACTGTGGCAAA+AGG | 0.503511 | 8.4:+16323688 | MS.gene80729:CDS |
| TGAAGCTACGACAGGAAAAG+AGG | 0.510943 | 8.4:+16323343 | MS.gene80729:CDS |
| AGAAATTGGTACTTGTTGTA+GGG | 0.511459 | 8.4:-16321989 | None:intergenic |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.515063 | 8.4:-16325598 | None:intergenic |
| TTGTTCTTGATGCGCTCACC+AGG | 0.522461 | 8.4:+16322204 | MS.gene80729:CDS |
| GTCTTCTTCTGGCTGAGCAT+CGG | 0.526811 | 8.4:-16329966 | None:intergenic |
| GACGATATCGACTGGGAGGA+AGG | 0.528222 | 8.4:+16329995 | MS.gene80729:CDS |
| TCTGCTGCGTTCGCATCTTG+GGG | 0.534141 | 8.4:-16328099 | None:intergenic |
| CGACTGACTTGATCTACTAA+AGG | 0.542394 | 8.4:-16325439 | None:intergenic |
| TGATGGTACCGAGTGGGAAG+AGG | 0.544924 | 8.4:+16328185 | MS.gene80729:CDS |
| ACGACGAGGACGATATCGAC+TGG | 0.550692 | 8.4:+16329987 | MS.gene80729:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.4:-16327737 | None:intergenic |
| TTGATTTCAAGCCAACTTGG+CGG | 0.551942 | 8.4:-16328146 | None:intergenic |
| TTTCAAGCCAACTTGGCGGT+TGG | 0.553993 | 8.4:-16328142 | None:intergenic |
| TTTAGTAGATCAAGTCAGTC+GGG | 0.559290 | 8.4:+16325441 | MS.gene80729:CDS |
| CAGGTGGTAGTTGACTTCAA+TGG | 0.563361 | 8.4:+16327650 | MS.gene80729:intron |
| GATAAGTTAGCTGCTCAAGA+CGG | 0.564672 | 8.4:+16323922 | MS.gene80729:CDS |
| TTTATAGGATGAGTATGTCA+AGG | 0.564885 | 8.4:+16328020 | MS.gene80729:intron |
| TATGACGATCTTACCAGCAA+AGG | 0.565230 | 8.4:+16322131 | MS.gene80729:CDS |
| AGTACTTCCAACCGCCAAGT+TGG | 0.569471 | 8.4:+16328135 | MS.gene80729:CDS |
| TGCAGATATATGATGTGGTT+AGG | 0.570661 | 8.4:+16323595 | MS.gene80729:intron |
| AACAGGAGCCTCTTCCCACT+CGG | 0.576584 | 8.4:-16328193 | None:intergenic |
| GTATCTCCGCTGTATGGCAA+TGG | 0.577878 | 8.4:-16325592 | None:intergenic |
| AAGCCCGAGCTAGTGCAGCT+GGG | 0.582139 | 8.4:+16325509 | MS.gene80729:CDS |
| AAACGTGAGGAAGATGATGA+TGG | 0.590028 | 8.4:+16328168 | MS.gene80729:CDS |
| CTTTAGTAGATCAAGTCAGT+CGG | 0.591765 | 8.4:+16325440 | MS.gene80729:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.599548 | 8.4:+16327659 | MS.gene80729:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.4:+16327671 | MS.gene80729:CDS |
| GTCTGTGAATTGCACTGATG+TGG | 0.605194 | 8.4:-16327825 | None:intergenic |
| GGTGATTCTAAATCTCATCT+GGG | 0.606736 | 8.4:+16325556 | MS.gene80729:CDS |
| GAAGCTACGACAGGAAAAGA+GGG | 0.609436 | 8.4:+16323344 | MS.gene80729:CDS |
| ATGGGTTAGAGAAGAAGACT+TGG | 0.611007 | 8.4:+16322328 | MS.gene80729:CDS |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.4:+16325535 | MS.gene80729:CDS |
| GCCATACAGCGGAGATACCA+AGG | 0.618161 | 8.4:+16325597 | MS.gene80729:CDS |
| CGACGAGGACGATATCGACT+GGG | 0.621959 | 8.4:+16329988 | MS.gene80729:CDS |
| CACTAGAGATCATAGGAGAG+AGG | 0.622575 | 8.4:+16322421 | MS.gene80729:CDS |
| CTGCTGTCTAGATTATGCAC+AGG | 0.622608 | 8.4:+16323400 | MS.gene80729:CDS |
| GCTACAGCTGAAGCTACGAC+AGG | 0.624340 | 8.4:+16323335 | MS.gene80729:CDS |
| AGATGATGATGGTACCGAGT+GGG | 0.630560 | 8.4:+16328179 | MS.gene80729:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.4:+16327665 | MS.gene80729:CDS |
| TAAGTTAGCTGCTCAAGACG+GGG | 0.634311 | 8.4:+16323924 | MS.gene80729:CDS |
| GAGCGCATCAAGAACAACTA+CGG | 0.638602 | 8.4:-16322198 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.4:+16327670 | MS.gene80729:CDS |
| ATGAGAATGCACGAAGACGA+CGG | 0.644965 | 8.4:+16323965 | MS.gene80729:CDS |
| TACGAAAGAACAGCGTGGAG+AGG | 0.645484 | 8.4:+16327766 | MS.gene80729:CDS |
| TTTGTTGCAGATATATGATG+TGG | 0.649334 | 8.4:+16323590 | MS.gene80729:intron |
| AGCAACTTCGTCGAACTCTG+CGG | 0.650459 | 8.4:+16322375 | MS.gene80729:CDS |
| GAATATATATGTACAAACTG+TGG | 0.654824 | 8.4:+16323681 | MS.gene80729:CDS |
| TGGCTTGAAATCAAAACGTG+AGG | 0.659414 | 8.4:+16328155 | MS.gene80729:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.661782 | 8.4:+16322334 | MS.gene80729:CDS |
| GGTGTTAAATCTGAAACTGA+CGG | 0.664295 | 8.4:+16327692 | MS.gene80729:CDS |
| TGCAATTGGAGCTTACAAGG+TGG | 0.682571 | 8.4:+16329928 | MS.gene80729:CDS |
| AAGTTAGCTGCTCAAGACGG+GGG | 0.688054 | 8.4:+16323925 | MS.gene80729:CDS |
| GGACATGCTTCAGAAGATGG+AGG | 0.689485 | 8.4:+16324002 | MS.gene80729:CDS |
| ACGTTGTAATGGAAAACTAG+AGG | 0.692315 | 8.4:+16323891 | MS.gene80729:CDS |
| GGAGCACCATTGCCATACAG+CGG | 0.718354 | 8.4:+16325586 | MS.gene80729:CDS |
| AAGATGATGATGGTACCGAG+TGG | 0.721118 | 8.4:+16328178 | MS.gene80729:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.4:+16322170 | MS.gene80729:CDS |
| TTCCATTACAACGTTCACAG+TGG | 0.729270 | 8.4:-16323882 | None:intergenic |
| CGAGGACGATATCGACTGGG+AGG | 0.742122 | 8.4:+16329991 | MS.gene80729:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.4:-16327734 | None:intergenic |
| AATCTTACGAAAGAACAGCG+TGG | 0.751641 | 8.4:+16327761 | MS.gene80729:CDS |
| TCAGCCAGAAGAAGACGACG+AGG | 0.751885 | 8.4:+16329973 | MS.gene80729:CDS |
| ACAATGCTTTGGATGCACTG+CGG | 0.757429 | 8.4:+16323833 | MS.gene80729:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 16321980 | 16330021 | 16321980 | ID=MS.gene80729 |
| chr8.4 | mRNA | 16321980 | 16330021 | 16321980 | ID=MS.gene80729.t1;Parent=MS.gene80729 |
| chr8.4 | exon | 16321980 | 16321999 | 16321980 | ID=MS.gene80729.t1.exon1;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16321980 | 16321999 | 16321980 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16322103 | 16322225 | 16322103 | ID=MS.gene80729.t1.exon2;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16322103 | 16322225 | 16322103 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16322322 | 16322442 | 16322322 | ID=MS.gene80729.t1.exon3;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16322322 | 16322442 | 16322322 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16323296 | 16323421 | 16323296 | ID=MS.gene80729.t1.exon4;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16323296 | 16323421 | 16323296 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16323600 | 16323709 | 16323600 | ID=MS.gene80729.t1.exon5;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16323600 | 16323709 | 16323600 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16323831 | 16324023 | 16323831 | ID=MS.gene80729.t1.exon6;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16323831 | 16324023 | 16323831 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16325427 | 16325618 | 16325427 | ID=MS.gene80729.t1.exon7;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16325427 | 16325618 | 16325427 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16327653 | 16327889 | 16327653 | ID=MS.gene80729.t1.exon8;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16327653 | 16327889 | 16327653 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16328027 | 16328219 | 16328027 | ID=MS.gene80729.t1.exon9;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16328027 | 16328219 | 16328027 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| chr8.4 | exon | 16329924 | 16330021 | 16329924 | ID=MS.gene80729.t1.exon10;Parent=MS.gene80729.t1 |
| chr8.4 | CDS | 16329924 | 16330021 | 16329924 | ID=cds.MS.gene80729.t1;Parent=MS.gene80729.t1 |
| Gene Sequence |
| Protein sequence |
