Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81792.t1 | XP_013447976.1 | 90.2 | 92 | 8 | 1 | 1 | 92 | 816 | 906 | 3.10E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81792.t1 | Q6F2Y7 | 69.6 | 79 | 24 | 0 | 1 | 79 | 814 | 892 | 1.2e-24 | 113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81792.t1 | A0A072TYS6 | 90.2 | 92 | 8 | 1 | 1 | 92 | 816 | 906 | 2.2e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene81792 | 0.819005 | 1.46E-52 | -1.69E-46 |
| MS.gene049435 | MS.gene81792 | 0.803548 | 3.31E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene81792 | 0.811483 | 6.84E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene81792 | 0.820342 | 7.22E-53 | -1.69E-46 |
| MS.gene050156 | MS.gene81792 | 0.806401 | 8.38E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene81792 | 0.80618 | 9.32E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene81792 | 0.816465 | 5.45E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene81792 | 0.811467 | 6.90E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene81792 | 0.807913 | 4.01E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene81792 | 0.835399 | 1.72E-56 | -1.69E-46 |
| MS.gene050844 | MS.gene81792 | 0.818066 | 2.38E-52 | -1.69E-46 |
| MS.gene050953 | MS.gene81792 | 0.820045 | 8.44E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene81792 | 0.821701 | 3.51E-53 | -1.69E-46 |
| MS.gene051265 | MS.gene81792 | 0.80686 | 6.70E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene81792 | 0.823964 | 1.04E-53 | -1.69E-46 |
| MS.gene052190 | MS.gene81792 | 0.831817 | 1.35E-55 | -1.69E-46 |
| MS.gene052208 | MS.gene81792 | 0.801639 | 8.19E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene81792 | 0.838067 | 3.58E-57 | -1.69E-46 |
| MS.gene052863 | MS.gene81792 | 0.832882 | 7.33E-56 | -1.69E-46 |
| MS.gene053008 | MS.gene81792 | 0.821475 | 3.96E-53 | -1.69E-46 |
| MS.gene053145 | MS.gene81792 | 0.809928 | 1.48E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene81792 | 0.827427 | 1.57E-54 | -1.69E-46 |
| MS.gene053226 | MS.gene81792 | 0.811764 | 5.95E-51 | -1.69E-46 |
| MS.gene053883 | MS.gene81792 | 0.820632 | 6.19E-53 | -1.69E-46 |
| MS.gene05414 | MS.gene81792 | 0.800465 | 1.42E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene81792 | 0.824215 | 9.11E-54 | -1.69E-46 |
| MS.gene054405 | MS.gene81792 | 0.81585 | 7.48E-52 | -1.69E-46 |
| MS.gene054467 | MS.gene81792 | 0.80329 | 3.74E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene81792 | 0.809199 | 2.13E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene81792 | 0.822189 | 2.71E-53 | -1.69E-46 |
| MS.gene054582 | MS.gene81792 | 0.800904 | 1.16E-48 | -1.69E-46 |
| MS.gene054651 | MS.gene81792 | 0.825616 | 4.25E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene81792 | 0.812246 | 4.67E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene81792 | 0.813278 | 2.78E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene81792 | 0.80142 | 9.08E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene81792 | 0.827912 | 1.20E-54 | -1.69E-46 |
| MS.gene055468 | MS.gene81792 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene81792 | 0.84354 | 1.32E-58 | -1.69E-46 |
| MS.gene055818 | MS.gene81792 | 0.855833 | 4.84E-62 | -1.69E-46 |
| MS.gene056173 | MS.gene81792 | 0.81722 | 3.69E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene81792 | 0.81919 | 1.32E-52 | -1.69E-46 |
| MS.gene056498 | MS.gene81792 | 0.844585 | 6.90E-59 | -1.69E-46 |
| MS.gene056595 | MS.gene81792 | 0.83845 | 2.85E-57 | -1.69E-46 |
| MS.gene057060 | MS.gene81792 | 0.805855 | 1.09E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene81792 | 0.81124 | 7.73E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene81792 | 0.846391 | 2.24E-59 | -1.69E-46 |
| MS.gene057693 | MS.gene81792 | 0.839835 | 1.25E-57 | -1.69E-46 |
| MS.gene058037 | MS.gene81792 | 0.808064 | 3.72E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene81792 | 0.838145 | 3.42E-57 | -1.69E-46 |
| MS.gene058636 | MS.gene81792 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene81792 | 0.81775 | 2.80E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene81792 | 0.81529 | 9.97E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene81792 | 0.804991 | 1.66E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene81792 | 0.800776 | 1.23E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene81792 | 0.807272 | 5.48E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene81792 | 0.808857 | 2.52E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene81792 | 0.870295 | 1.63E-66 | -1.69E-46 |
| MS.gene060521 | MS.gene81792 | 0.809603 | 1.74E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene81792 | 0.808557 | 2.92E-50 | -1.69E-46 |
| MS.gene060685 | MS.gene81792 | 0.800518 | 1.39E-48 | -1.69E-46 |
| MS.gene060781 | MS.gene81792 | 0.806585 | 7.66E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene81792 | 0.810715 | 1.00E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene81792 | -0.809109 | 2.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81792.t1 | MTR_7g028970 | 90.217 | 92 | 8 | 1 | 1 | 92 | 816 | 906 | 3.24e-49 | 166 |
| MS.gene81792.t1 | MTR_4g109450 | 89.610 | 77 | 8 | 0 | 1 | 77 | 816 | 892 | 1.28e-41 | 144 |
| MS.gene81792.t1 | MTR_7g096480 | 84.722 | 72 | 11 | 0 | 1 | 72 | 172 | 243 | 5.51e-37 | 124 |
| MS.gene81792.t1 | MTR_2g006950 | 75.641 | 78 | 19 | 0 | 1 | 78 | 510 | 587 | 2.59e-32 | 117 |
| MS.gene81792.t1 | MTR_2g099075 | 67.213 | 61 | 19 | 1 | 1 | 60 | 411 | 471 | 2.48e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81792.t1 | AT1G74310 | 69.231 | 78 | 21 | 2 | 1 | 78 | 813 | 887 | 5.15e-29 | 108 |
| MS.gene81792.t1 | AT4G14675 | 51.923 | 52 | 25 | 0 | 1 | 52 | 65 | 116 | 1.65e-15 | 67.4 |
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCAAGCCACCTTCTAAT+TGG | 0.289169 | 7.2:+70737975 | None:intergenic |
| GCTATCTATGATGCTTATAA+AGG | 0.353907 | 7.2:-70737937 | MS.gene81792:CDS |
| TTTATTGTTGTGTAGGTGTA+TGG | 0.363041 | 7.2:-70738005 | None:intergenic |
| GGGATTGTCGATGCTGAAAC+TGG | 0.476416 | 7.2:-70737831 | MS.gene81792:CDS |
| GAAAATGGATATGAGGTCAT+TGG | 0.492246 | 7.2:-70737756 | MS.gene81792:CDS |
| TATAACAATTCACTTCCCTT+AGG | 0.506610 | 7.2:+70737873 | None:intergenic |
| ATGCATTGATGCTGGTCCTA+AGG | 0.506976 | 7.2:-70737889 | MS.gene81792:CDS |
| GGTCATTGGCGATGATGAAA+TGG | 0.512438 | 7.2:-70737742 | MS.gene81792:CDS |
| TTATACCGTGTGGAAAAGAA+TGG | 0.527875 | 7.2:-70737855 | MS.gene81792:CDS |
| TGACGATGAAAATGGATATG+AGG | 0.542396 | 7.2:-70737763 | MS.gene81792:CDS |
| TGCATTGATGCTGGTCCTAA+GGG | 0.583971 | 7.2:-70737888 | MS.gene81792:CDS |
| TACCGTGTGGAAAAGAATGG+AGG | 0.589660 | 7.2:-70737852 | MS.gene81792:CDS |
| ACCGTGTGGAAAAGAATGGA+GGG | 0.604577 | 7.2:-70737851 | MS.gene81792:CDS |
| ACAACTGTATGCATTGATGC+TGG | 0.605777 | 7.2:-70737897 | MS.gene81792:CDS |
| AAGTGAATTGTTATACCGTG+TGG | 0.624493 | 7.2:-70737865 | MS.gene81792:CDS |
| ATGGTGCTAGACCAATTAGA+AGG | 0.648738 | 7.2:-70737986 | MS.gene81792:CDS |
| GTGCTAGACCAATTAGAAGG+TGG | 0.668833 | 7.2:-70737983 | MS.gene81792:CDS |
| AAGGTGGCTTGAGAAGAATG+TGG | 0.734759 | 7.2:-70737967 | MS.gene81792:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAATTCAGATACCAAA+TGG | - | chr7.2:70737929-70737948 | MS.gene81792:CDS | 20.0% |
| GCTATCTATGATGCTTATAA+AGG | - | chr7.2:70737787-70737806 | MS.gene81792:CDS | 30.0% | |
| TATAACAATTCACTTCCCTT+AGG | + | chr7.2:70737854-70737873 | None:intergenic | 30.0% | |
| AAGTGAATTGTTATACCGTG+TGG | - | chr7.2:70737859-70737878 | MS.gene81792:CDS | 35.0% | |
| CCAAAAACTGACGATGAAAA+TGG | - | chr7.2:70737953-70737972 | MS.gene81792:CDS | 35.0% | |
| GAAAATGGATATGAGGTCAT+TGG | - | chr7.2:70737968-70737987 | MS.gene81792:CDS | 35.0% | |
| TGACGATGAAAATGGATATG+AGG | - | chr7.2:70737961-70737980 | MS.gene81792:CDS | 35.0% | |
| TTATACCGTGTGGAAAAGAA+TGG | - | chr7.2:70737869-70737888 | MS.gene81792:CDS | 35.0% | |
| ! | CCATTTTCATCGTCAGTTTT+TGG | + | chr7.2:70737956-70737975 | None:intergenic | 35.0% |
| TTCTCAAGCCACCTTCTAAT+TGG | + | chr7.2:70737752-70737771 | None:intergenic | 40.0% | |
| ! | ACAACTGTATGCATTGATGC+TGG | - | chr7.2:70737827-70737846 | MS.gene81792:CDS | 40.0% |
| ! | ATGGTGCTAGACCAATTAGA+AGG | - | chr7.2:70737738-70737757 | MS.gene81792:CDS | 40.0% |
| !!! | TCGTCAGTTTTTGGTCCATT+TGG | + | chr7.2:70737947-70737966 | None:intergenic | 40.0% |
| AAGGTGGCTTGAGAAGAATG+TGG | - | chr7.2:70737757-70737776 | MS.gene81792:CDS | 45.0% | |
| ACCGTGTGGAAAAGAATGGA+GGG | - | chr7.2:70737873-70737892 | MS.gene81792:CDS | 45.0% | |
| GGTCATTGGCGATGATGAAA+TGG | - | chr7.2:70737982-70738001 | MS.gene81792:CDS | 45.0% | |
| GTGCTAGACCAATTAGAAGG+TGG | - | chr7.2:70737741-70737760 | MS.gene81792:CDS | 45.0% | |
| TACCGTGTGGAAAAGAATGG+AGG | - | chr7.2:70737872-70737891 | MS.gene81792:CDS | 45.0% | |
| ! | ATGCATTGATGCTGGTCCTA+AGG | - | chr7.2:70737835-70737854 | MS.gene81792:CDS | 45.0% |
| ! | TCCCTCCATTCTTTTCCACA+CGG | + | chr7.2:70737877-70737896 | None:intergenic | 45.0% |
| ! | TGCATTGATGCTGGTCCTAA+GGG | - | chr7.2:70737836-70737855 | MS.gene81792:CDS | 45.0% |
| GGGATTGTCGATGCTGAAAC+TGG | - | chr7.2:70737893-70737912 | MS.gene81792:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 70737734 | 70738012 | 70737734 | ID=MS.gene81792 |
| chr7.2 | mRNA | 70737734 | 70738012 | 70737734 | ID=MS.gene81792.t1;Parent=MS.gene81792 |
| chr7.2 | exon | 70737734 | 70738012 | 70737734 | ID=MS.gene81792.t1.exon1;Parent=MS.gene81792.t1 |
| chr7.2 | CDS | 70737734 | 70738012 | 70737734 | ID=cds.MS.gene81792.t1;Parent=MS.gene81792.t1 |
| Gene Sequence |
| Protein sequence |
