AlfalfaGEDB | MS.gene82145

M. sativa cultivar XinJiangDaYe / MS.gene82145

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016740 GO:0016746 GO:0016747
PFAM: PF02458.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82145.t1	XP_003612550.1	96.1	458	18	0	1	458	1	458	4.80E-256	893.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82145.t1	Q9FFQ7	24.4	401	269	11	14	401	3	382	1.5e-19	99.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82145.t1	G7K5F8	96.1	458	18	0	1	458	1	458	3.5e-256	893.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049190	MS.gene82145	0.822674	2.09E-53	-1.69E-46
MS.gene049254	MS.gene82145	0.808251	3.40E-50	-1.69E-46
MS.gene049711	MS.gene82145	0.841927	3.53E-58	-1.69E-46
MS.gene049824	MS.gene82145	0.815479	9.05E-52	-1.69E-46
MS.gene049825	MS.gene82145	0.802222	6.21E-49	-1.69E-46
MS.gene049850	MS.gene82145	0.801362	9.33E-49	-1.69E-46
MS.gene049873	MS.gene82145	0.824448	8.03E-54	-1.69E-46
MS.gene05016	MS.gene82145	0.851064	1.13E-60	-1.69E-46
MS.gene050559	MS.gene82145	0.828733	7.63E-55	-1.69E-46
MS.gene050561	MS.gene82145	0.822341	2.49E-53	-1.69E-46
MS.gene050562	MS.gene82145	0.81288	3.39E-51	-1.69E-46
MS.gene051279	MS.gene82145	0.819169	1.34E-52	-1.69E-46
MS.gene051719	MS.gene82145	0.850445	1.69E-60	-1.69E-46
MS.gene051720	MS.gene82145	0.822257	2.61E-53	-1.69E-46
MS.gene051744	MS.gene82145	0.847899	8.64E-60	-1.69E-46
MS.gene051957	MS.gene82145	0.807053	6.10E-50	-1.69E-46
MS.gene052020	MS.gene82145	0.802154	6.42E-49	-1.69E-46
MS.gene052519	MS.gene82145	0.802993	4.31E-49	-1.69E-46
MS.gene052582	MS.gene82145	0.801055	1.08E-48	-1.69E-46
MS.gene052611	MS.gene82145	0.839073	1.97E-57	-1.69E-46
MS.gene052612	MS.gene82145	0.818478	1.92E-52	-1.69E-46
MS.gene053144	MS.gene82145	0.801924	7.16E-49	-1.69E-46
MS.gene053226	MS.gene82145	0.823073	1.69E-53	-1.69E-46
MS.gene053599	MS.gene82145	0.80712	5.90E-50	-1.69E-46
MS.gene053998	MS.gene82145	0.808154	3.56E-50	-1.69E-46
MS.gene054651	MS.gene82145	0.825832	3.78E-54	-1.69E-46
MS.gene055227	MS.gene82145	0.803013	4.27E-49	-1.69E-46
MS.gene055778	MS.gene82145	0.809729	1.64E-50	-1.69E-46
MS.gene055818	MS.gene82145	0.831354	1.75E-55	-1.69E-46
MS.gene056050	MS.gene82145	0.817919	2.57E-52	-1.69E-46
MS.gene056366	MS.gene82145	0.830187	3.38E-55	-1.69E-46
MS.gene056542	MS.gene82145	0.818043	2.41E-52	-1.69E-46
MS.gene056543	MS.gene82145	0.819735	9.94E-53	-1.69E-46
MS.gene056545	MS.gene82145	0.818053	2.40E-52	-1.69E-46
MS.gene056553	MS.gene82145	0.81997	8.78E-53	-1.69E-46
MS.gene05686	MS.gene82145	0.810332	1.22E-50	-1.69E-46
MS.gene057026	MS.gene82145	0.802442	5.60E-49	-1.69E-46
MS.gene057060	MS.gene82145	0.808836	2.55E-50	-1.69E-46
MS.gene058625	MS.gene82145	0.856074	4.11E-62	-1.69E-46
MS.gene058829	MS.gene82145	0.832053	1.18E-55	-1.69E-46
MS.gene058979	MS.gene82145	0.808954	2.40E-50	-1.69E-46
MS.gene059015	MS.gene82145	0.843369	1.46E-58	-1.69E-46
MS.gene059154	MS.gene82145	0.814188	1.75E-51	-1.69E-46
MS.gene061163	MS.gene82145	0.816815	4.55E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene038271	MS.gene82145	PPI
MS.gene019178	MS.gene82145	PPI
MS.gene017058	MS.gene82145	PPI
MS.gene038274	MS.gene82145	PPI
MS.gene47721	MS.gene82145	PPI
MS.gene000507	MS.gene82145	PPI
MS.gene69496	MS.gene82145	PPI
MS.gene22199	MS.gene82145	PPI
MS.gene017054	MS.gene82145	PPI
MS.gene48027	MS.gene82145	PPI
MS.gene017053	MS.gene82145	PPI
MS.gene47716	MS.gene82145	PPI
MS.gene47719	MS.gene82145	PPI
MS.gene48021	MS.gene82145	PPI
MS.gene48023	MS.gene82145	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82145.t1	MTR_5g026320	96.070	458	18	0	1	458	1	458	0.0	914
MS.gene82145.t1	MTR_3g450710	36.215	428	246	11	53	456	44	468	8.32e-75	242
MS.gene82145.t1	MTR_5g090830	36.219	439	249	14	15	428	7	439	3.04e-73	238
MS.gene82145.t1	MTR_5g090810	35.927	437	253	14	15	428	7	439	1.34e-72	236
MS.gene82145.t1	MTR_3g450690	36.650	412	237	10	67	457	65	473	1.20e-71	234
MS.gene82145.t1	MTR_5g090880	38.095	399	218	13	56	428	46	441	4.54e-71	233
MS.gene82145.t1	MTR_1g053315	25.412	425	284	15	34	437	35	447	8.75e-27	112
MS.gene82145.t1	MTR_4g007540	25.178	421	280	13	42	444	25	428	1.81e-25	108
MS.gene82145.t1	MTR_7g114470	25.227	440	270	17	29	437	20	431	2.83e-25	108
MS.gene82145.t1	MTR_5g076700	24.336	452	282	16	16	437	8	429	4.64e-25	107
MS.gene82145.t1	MTR_8g096530	28.107	338	214	12	41	361	28	353	1.26e-24	106
MS.gene82145.t1	MTR_2g064495	23.380	432	297	15	22	437	7	420	3.54e-24	104
MS.gene82145.t1	MTR_3g098980	24.876	402	261	14	41	424	35	413	1.34e-23	103
MS.gene82145.t1	MTR_2g105330	23.733	434	296	16	21	437	5	420	2.99e-23	102
MS.gene82145.t1	MTR_3g049150	24.819	415	279	11	52	443	36	440	7.19e-23	101
MS.gene82145.t1	MTR_5g084870	24.471	425	277	13	17	424	2	399	7.52e-23	100
MS.gene82145.t1	MTR_4g022290	26.068	468	278	23	17	447	3	439	9.20e-23	100
MS.gene82145.t1	MTR_4g127650	24.631	406	271	12	19	397	9	406	7.85e-22	98.2
MS.gene82145.t1	MTR_7g027175	26.260	377	242	14	41	401	37	393	8.51e-22	97.8
MS.gene82145.t1	MTR_7g084940	25.995	377	240	13	41	399	37	392	1.20e-21	97.4
MS.gene82145.t1	MTR_4g097090	25.464	377	236	15	53	397	42	405	1.28e-21	97.4
MS.gene82145.t1	MTR_3g112180	25.500	400	247	13	33	405	25	400	2.65e-21	96.7
MS.gene82145.t1	MTR_8g079970	23.744	438	272	17	17	426	2	405	8.69e-21	94.7
MS.gene82145.t1	MTR_7g086000	24.402	418	279	15	55	443	41	450	1.21e-20	94.7
MS.gene82145.t1	MTR_7g085990	27.209	430	242	21	32	417	18	420	1.33e-20	94.4
MS.gene82145.t1	MTR_8g075610	24.332	374	256	13	42	401	16	376	1.44e-20	94.0
MS.gene82145.t1	MTR_7g114550	25.888	394	249	15	32	404	22	393	1.57e-20	94.4
MS.gene82145.t1	MTR_1g040215	26.716	408	253	19	16	402	7	389	4.14e-20	92.8
MS.gene82145.t1	MTR_4g094828	26.076	395	241	18	37	400	25	399	1.75e-19	90.9
MS.gene82145.t1	MTR_7g114560	24.173	393	257	15	32	404	22	393	1.78e-19	90.9
MS.gene82145.t1	MTR_7g114520	24.557	395	255	14	32	404	23	396	2.01e-19	90.9
MS.gene82145.t1	MTR_8g079980	24.490	441	264	14	17	426	2	404	3.75e-19	89.7
MS.gene82145.t1	MTR_8g079950	23.516	438	271	17	17	424	2	405	9.08e-19	89.0
MS.gene82145.t1	MTR_3g101900	26.506	415	262	17	58	448	48	443	1.07e-18	88.6
MS.gene82145.t1	MTR_7g114490	24.289	387	260	13	32	402	22	391	1.21e-18	88.6
MS.gene82145.t1	MTR_7g085930	25.659	417	222	19	56	417	41	424	4.62e-18	86.7
MS.gene82145.t1	MTR_3g086020	23.913	414	238	18	15	400	2	366	6.23e-18	85.9
MS.gene82145.t1	MTR_4g094842	23.542	463	299	17	18	448	4	443	6.66e-18	86.3
MS.gene82145.t1	MTR_0190s0020	25.325	462	286	20	18	448	4	437	7.35e-18	85.9
MS.gene82145.t1	MTR_3g101960	25.612	449	282	17	34	448	15	445	7.83e-18	85.9
MS.gene82145.t1	MTR_8g467590	26.316	380	219	22	51	400	38	386	1.32e-17	85.1
MS.gene82145.t1	MTR_1g040175	26.531	392	242	18	33	402	22	389	2.48e-17	84.3
MS.gene82145.t1	MTR_8g467580	25.526	380	230	15	47	400	34	386	6.20e-16	80.1
MS.gene82145.t1	MTR_6g015815	26.353	425	250	19	41	424	34	436	6.57e-16	80.1
MS.gene82145.t1	MTR_7g056087	23.433	367	251	13	18	361	4	363	1.26e-15	79.0
MS.gene82145.t1	MTR_4g094835	23.529	408	255	17	71	448	61	441	1.53e-15	79.0
MS.gene82145.t1	MTR_3g071320	24.292	424	282	16	23	423	8	415	2.65e-15	78.2
MS.gene82145.t1	MTR_5g084850	22.897	428	281	13	17	426	2	398	5.28e-15	77.4
MS.gene82145.t1	MTR_1g040315	24.818	274	177	9	16	272	7	268	6.50e-15	76.6
MS.gene82145.t1	MTR_1g081660	24.233	326	216	12	41	347	39	352	1.67e-14	75.9
MS.gene82145.t1	MTR_7g114960	21.944	319	225	11	56	361	45	352	6.10e-14	73.9
MS.gene82145.t1	MTR_7g084910	24.339	378	231	14	41	399	38	379	1.60e-13	72.8
MS.gene82145.t1	MTR_3g026950	20.755	424	299	13	35	430	17	431	1.83e-13	72.4
MS.gene82145.t1	MTR_8g079710	24.163	418	255	22	18	400	4	394	2.44e-13	72.0
MS.gene82145.t1	MTR_3g465910	22.841	359	231	10	12	340	2	344	5.54e-13	71.2
MS.gene82145.t1	MTR_3g465930	23.513	353	239	12	12	340	2	347	6.24e-13	70.9
MS.gene82145.t1	MTR_6g053810	23.969	388	218	13	14	355	12	368	6.53e-13	70.9
MS.gene82145.t1	MTR_6g015850	24.074	432	262	19	10	399	2	409	9.96e-13	70.1
MS.gene82145.t1	MTR_2g015430	21.053	342	240	12	74	400	58	384	1.31e-12	69.7
MS.gene82145.t1	MTR_4g094832	24.595	370	228	16	103	448	83	425	1.86e-12	69.3
MS.gene82145.t1	MTR_6g015315	25.604	414	259	17	45	424	30	428	1.90e-12	69.3
MS.gene82145.t1	MTR_1g094790	24.824	427	241	22	17	400	2	391	1.91e-12	69.3
MS.gene82145.t1	MTR_2g036780	27.459	244	158	10	40	272	34	269	1.98e-12	69.3
MS.gene82145.t1	MTR_3g100150	26.172	256	155	10	37	272	30	271	3.83e-11	65.5
MS.gene82145.t1	MTR_3g465880	22.400	375	246	12	13	355	3	364	6.28e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82145.t1	AT1G31490	58.296	446	176	8	16	458	4	442	0.0	555
MS.gene82145.t1	AT1G78990	39.633	381	218	10	57	430	50	425	2.25e-79	254
MS.gene82145.t1	AT5G16410	36.092	435	257	12	8	430	26	451	5.23e-74	241
MS.gene82145.t1	AT1G32910	36.990	392	226	12	53	430	46	430	2.22e-73	238
MS.gene82145.t1	AT5G41040	27.733	375	241	11	41	401	38	396	2.15e-27	114
MS.gene82145.t1	AT5G41040	27.733	375	241	11	41	401	54	412	3.02e-27	114
MS.gene82145.t1	AT1G27620	25.581	387	247	14	32	400	21	384	1.29e-23	103
MS.gene82145.t1	AT3G26040	26.484	438	238	15	17	420	2	389	1.44e-23	103
MS.gene82145.t1	AT5G63560	24.688	401	268	12	14	401	3	382	1.42e-22	100
MS.gene82145.t1	AT2G19070	26.263	396	232	13	48	397	20	401	1.95e-22	99.8
MS.gene82145.t1	AT3G48720	24.304	395	227	14	41	401	30	386	1.96e-22	99.8
MS.gene82145.t1	AT1G24430	25.537	419	262	17	27	428	18	403	4.87e-22	98.6
MS.gene82145.t1	AT5G57840	25.070	359	244	12	17	360	2	350	7.11e-22	98.2
MS.gene82145.t1	AT1G03390	26.276	392	242	19	35	400	37	407	1.04e-21	97.8
MS.gene82145.t1	AT3G30280	24.703	421	271	19	14	411	2	399	2.74e-21	96.3
MS.gene82145.t1	AT5G42830	23.628	419	270	15	58	448	48	444	1.05e-20	94.7
MS.gene82145.t1	AT5G47950	23.598	428	282	17	14	428	2	397	1.58e-20	94.0
MS.gene82145.t1	AT5G48930	24.943	437	281	16	24	437	9	421	3.29e-20	93.2
MS.gene82145.t1	AT3G50270	24.086	465	286	20	18	445	6	440	1.90e-19	90.9
MS.gene82145.t1	AT2G40230	24.810	395	250	16	35	404	20	392	3.79e-19	89.7
MS.gene82145.t1	AT5G38130	24.183	459	299	16	17	447	23	460	1.19e-18	88.6
MS.gene82145.t1	AT1G24420	28.788	330	193	15	18	327	5	312	2.58e-17	84.3
MS.gene82145.t1	AT1G24420	28.788	330	193	15	18	327	5	312	2.58e-17	84.3
MS.gene82145.t1	AT5G67150	24.528	424	249	20	17	400	4	396	3.26e-17	84.0
MS.gene82145.t1	AT4G15390	24.146	410	268	16	12	400	2	389	3.37e-17	84.0
MS.gene82145.t1	AT4G15390	24.146	410	268	16	12	400	2	389	3.37e-17	84.0
MS.gene82145.t1	AT3G50280	24.760	416	256	20	17	400	3	393	3.57e-16	80.9
MS.gene82145.t1	AT1G65450	23.016	378	255	15	41	399	40	400	4.52e-16	80.5
MS.gene82145.t1	AT4G31910	27.000	300	181	13	30	297	23	316	9.09e-16	79.7
MS.gene82145.t1	AT5G67160	24.401	459	287	18	17	449	4	428	5.87e-15	77.0
MS.gene82145.t1	AT5G07860	20.551	399	279	10	68	448	66	444	2.64e-14	75.1
MS.gene82145.t1	AT1G28680	22.674	344	231	10	74	400	62	387	9.99e-14	73.6
MS.gene82145.t1	AT2G25150	24.290	317	189	10	66	352	59	354	3.17e-13	72.0
MS.gene82145.t1	AT5G07850	22.814	469	310	17	17	454	7	454	9.19e-13	70.5
MS.gene82145.t1	AT1G28680	22.442	303	209	8	74	360	62	354	4.02e-12	68.2

Find 78 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGAGACTTGCTTCTGTTTA+TGG	0.091737	5.2:+16996716	MS.gene82145:CDS
TCTCAAATACCAACCTTATT+TGG	0.132764	5.2:+16996583	MS.gene82145:CDS
GAGAGATTAATCACTGAAAA+TGG	0.231244	5.2:-16996071	None:intergenic
TCAAATCTTGATCTACTTTC+AGG	0.243930	5.2:+16996092	MS.gene82145:CDS
TCCTGTTAGTCAAGTTGATT+TGG	0.253495	5.2:+16997129	MS.gene82145:CDS
CATCGGTAATGTATTGTCTT+TGG	0.264157	5.2:+16996892	MS.gene82145:CDS
GGACTGTTTCTGCTATCTTA+TGG	0.267844	5.2:+16997251	MS.gene82145:CDS
GGATTTGGAAGTCCTTTGTT+AGG	0.289603	5.2:+16997151	MS.gene82145:CDS
AGCTTCAATCTTTGTTCTCT+TGG	0.297597	5.2:-16996741	None:intergenic
TTCAAAGCATCTACTAATTC+AGG	0.309519	5.2:-16997274	None:intergenic
AGTCAAGTTGATTTGGGATT+TGG	0.311632	5.2:+16997136	MS.gene82145:CDS
CCTGTCTTAGTGGTTTCTTC+AGG	0.314491	5.2:+16997097	MS.gene82145:CDS
AGATTTCCCTTTACAAATTC+AGG	0.320821	5.2:+16996394	MS.gene82145:CDS
TAGAAATACAAGTATGTAAC+TGG	0.322652	5.2:-16996122	None:intergenic
TGAATTTGTAAAGGGAAATC+TGG	0.334806	5.2:-16996392	None:intergenic
TTGATTGGATTGAGTTTCAT+AGG	0.350449	5.2:+16997029	MS.gene82145:CDS
CCTGTTAGTCAAGTTGATTT+GGG	0.352989	5.2:+16997130	MS.gene82145:CDS
CCCAAATCAACTTGACTAAC+AGG	0.353215	5.2:-16997130	None:intergenic
GATTTGAAGTTACCTAGTTT+TGG	0.354372	5.2:-16996152	None:intergenic
ATGCTTGCAAAGGCGGTGTT+AGG	0.354998	5.2:+16997061	MS.gene82145:CDS
TTAGGTGATGCAACTTCATT+TGG	0.362113	5.2:+16996470	MS.gene82145:CDS
ATTACTTGGATTTGATTGAT+TGG	0.362571	5.2:+16997014	MS.gene82145:CDS
CTGTTACCTGAATTTGTAAA+GGG	0.364202	5.2:-16996400	None:intergenic
AATCATTACTATCCCTTTGC+TGG	0.366290	5.2:+16996215	MS.gene82145:CDS
TTAATCACTGAAAATGGCTT+TGG	0.389224	5.2:-16996065	None:intergenic
AATGTATTGTCTTTGGCTTT+CGG	0.391960	5.2:+16996899	MS.gene82145:CDS
AAGCTTTCTCTGCATATGTA+TGG	0.403699	5.2:+16996759	MS.gene82145:CDS
GTGGTAAGGGTGATGGTTCA+TGG	0.408074	5.2:+16997230	MS.gene82145:CDS
TTGTTGTCACAAACGATTTG+AGG	0.427819	5.2:-16996269	None:intergenic
TTGGTAAATTCATTTCATCT+TGG	0.434237	5.2:+16996489	MS.gene82145:CDS
TGAATTAGTAGATGCTTTGA+AGG	0.434332	5.2:+16997276	MS.gene82145:CDS
TCTGTTACCTGAATTTGTAA+AGG	0.444738	5.2:-16996401	None:intergenic
TAGTAATGAGGAACATTACT+TGG	0.453243	5.2:+16997000	MS.gene82145:CDS
AAGATGGGTTGGTTAGTAGA+TGG	0.454588	5.2:+16996827	MS.gene82145:CDS
CTGAAGATGAGAAGCAGTCA+TGG	0.458441	5.2:-16997317	None:intergenic
AACACCAACCGTTTCAATTG+AGG	0.458953	5.2:-16997185	None:intergenic
GAATCAAAGGCAAAGTGGTA+AGG	0.464027	5.2:+16997216	MS.gene82145:CDS
TGGTTCGGTATGCATGATAA+CGG	0.476676	5.2:-16996533	None:intergenic
TTGAACAATGTGACCAGCAA+AGG	0.477158	5.2:-16996228	None:intergenic
GATTTGGAAGTCCTTTGTTA+GGG	0.486362	5.2:+16997152	MS.gene82145:CDS
CTTTGGCTTTCGGTGAGGCA+AGG	0.495189	5.2:+16996909	MS.gene82145:CDS
TGGATTGAGTTTCATAGGCC+AGG	0.499721	5.2:+16997034	MS.gene82145:CDS
AGATGATGGACCTGTCTTAG+TGG	0.507067	5.2:+16997087	MS.gene82145:CDS
ACAAGTATGTAACTGGAAAA+CGG	0.512372	5.2:-16996115	None:intergenic
CATTGTTCAAAATCATAAAA+CGG	0.517841	5.2:+16996241	MS.gene82145:CDS
AATCAAAGGCAAAGTGGTAA+GGG	0.520980	5.2:+16997217	MS.gene82145:CDS
TGAACAATGTGACCAGCAAA+GGG	0.522796	5.2:-16996227	None:intergenic
AGGTTTGATGCTTGCAAAGG+CGG	0.529986	5.2:+16997054	MS.gene82145:CDS
AGTTCATGATGCAATTTCAA+AGG	0.532962	5.2:+16996976	MS.gene82145:CDS
AATTTCAAAGGTTAGTAATG+AGG	0.533878	5.2:+16996988	MS.gene82145:CDS
GATGGAAGAGAAAGAATGAG+TGG	0.544115	5.2:+16996845	MS.gene82145:CDS
GTAACAGAATTTACTTGTGG+TGG	0.546469	5.2:+16996416	MS.gene82145:CDS
AAATTTGATCCAAATAAGGT+TGG	0.546882	5.2:-16996592	None:intergenic
TCAATTGAAACGGTTGGTGT+TGG	0.548363	5.2:+16997187	MS.gene82145:CDS
GGCAAGGATTCAAGAATTGA+AGG	0.552829	5.2:+16996925	MS.gene82145:CDS
AGTGTATACCTCAATTGAAA+CGG	0.556506	5.2:+16997177	MS.gene82145:CDS
GCCTTTGCAAGCATCAAACC+TGG	0.556923	5.2:-16997052	None:intergenic
GTGTTGGTTATATGAATCAA+AGG	0.560599	5.2:+16997203	MS.gene82145:CDS
CCTGAAGAAACCACTAAGAC+AGG	0.567163	5.2:-16997097	None:intergenic
TAGTGGTTTCTTCAGGACAA+AGG	0.574621	5.2:+16997104	MS.gene82145:CDS
TGAGAGGCGAACGTGCGCAA+AGG	0.578264	5.2:-16996565	None:intergenic
TGAAGATGAGAAGCAGTCAT+GGG	0.589116	5.2:-16997316	None:intergenic
AGGATTCAAGAATTGAAGGA+AGG	0.590702	5.2:+16996929	MS.gene82145:CDS
TCTTGAAGAAAAGATAGTCT+CGG	0.591038	5.2:+16996364	MS.gene82145:CDS
GCCAGGTTTGATGCTTGCAA+AGG	0.594613	5.2:+16997051	MS.gene82145:CDS
AGGCAAAGTGGTAAGGGTGA+TGG	0.604002	5.2:+16997223	MS.gene82145:CDS
AAATAAGGTTGGTATTTGAG+AGG	0.609809	5.2:-16996581	None:intergenic
GAAGGTTCATTATCAGACAT+AGG	0.615379	5.2:+16996947	MS.gene82145:CDS
CTGCAGCATGTTAATACTCG+AGG	0.620321	5.2:-16996696	None:intergenic
GCGGTGTTAGGACAAGATGA+TGG	0.622280	5.2:+16997073	MS.gene82145:CDS
ATTGTCTTTGGCTTTCGGTG+AGG	0.622701	5.2:+16996904	MS.gene82145:CDS
CAGGTAACAGAATTTACTTG+TGG	0.623975	5.2:+16996413	MS.gene82145:CDS
TATACCTCAATTGAAACGGT+TGG	0.626827	5.2:+16997181	MS.gene82145:CDS
AATTCAATGTCGAATTACAT+CGG	0.628778	5.2:+16996875	MS.gene82145:CDS
TATATGAATCAAAGGCAAAG+TGG	0.632585	5.2:+16997211	MS.gene82145:CDS
CAAATCGTTTGTGACAACAA+TGG	0.655714	5.2:+16996272	MS.gene82145:CDS
GTATACACTGTCCCTAACAA+AGG	0.661728	5.2:-16997163	None:intergenic
ATGGAAGAAAATGATAAACA+CGG	0.726903	5.2:+16996778	MS.gene82145:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAAATTTGATCCAAATA+AGG	-	chr5.2:16996599-16996618	None:intergenic	15.0%
!!	TAAAAATCTAAATTACTTAG+TGG	-	chr5.2:16996329-16996348	None:intergenic	15.0%
!!!	TTCAAATTTTTCATATTCTT+GGG	-	chr5.2:16996000-16996019	None:intergenic	15.0%
!!	CATTGTTCAAAATCATAAAA+CGG	+	chr5.2:16996241-16996260	MS.gene82145:CDS	20.0%
!!!	CTTCAAATTTTTCATATTCT+TGG	-	chr5.2:16996001-16996020	None:intergenic	20.0%
!	AAATTTGATCCAAATAAGGT+TGG	-	chr5.2:16996595-16996614	None:intergenic	25.0%
!	AATTCAATGTCGAATTACAT+CGG	+	chr5.2:16996875-16996894	MS.gene82145:CDS	25.0%
!	AATTTCAAAGGTTAGTAATG+AGG	+	chr5.2:16996988-16997007	MS.gene82145:CDS	25.0%
!	ACTTCATAAAAAATGCAAGA+TGG	+	chr5.2:16996811-16996830	MS.gene82145:CDS	25.0%
!	ATGGAAGAAAATGATAAACA+CGG	+	chr5.2:16996778-16996797	MS.gene82145:CDS	25.0%
!	ATTACTTGGATTTGATTGAT+TGG	+	chr5.2:16997014-16997033	MS.gene82145:CDS	25.0%
!	CTTCATAAAAAATGCAAGAT+GGG	+	chr5.2:16996812-16996831	MS.gene82145:CDS	25.0%
!	TAGAAATACAAGTATGTAAC+TGG	-	chr5.2:16996125-16996144	None:intergenic	25.0%
!	TTCTACAAAAAACCAAAACT+AGG	+	chr5.2:16996140-16996159	MS.gene82145:CDS	25.0%
!	TTGGTAAATTCATTTCATCT+TGG	+	chr5.2:16996489-16996508	MS.gene82145:CDS	25.0%
!!	AATTTTTATCAAATGCACCA+TGG	+	chr5.2:16996610-16996629	MS.gene82145:CDS	25.0%
!!!	TTTACTTTTGATCATGCATT+AGG	+	chr5.2:16996452-16996471	MS.gene82145:CDS	25.0%
	ACAAGTATGTAACTGGAAAA+CGG	-	chr5.2:16996118-16996137	None:intergenic	30.0%
	AGATTTCCCTTTACAAATTC+AGG	+	chr5.2:16996394-16996413	MS.gene82145:CDS	30.0%
	AGTGTATACCTCAATTGAAA+CGG	+	chr5.2:16997177-16997196	MS.gene82145:CDS	30.0%
	AGTTCATGATGCAATTTCAA+AGG	+	chr5.2:16996976-16996995	MS.gene82145:CDS	30.0%
	ATAAAAAATGCAAGATGGGT+TGG	+	chr5.2:16996816-16996835	MS.gene82145:CDS	30.0%
	CTGTTACCTGAATTTGTAAA+GGG	-	chr5.2:16996403-16996422	None:intergenic	30.0%
	GAGAGATTAATCACTGAAAA+TGG	-	chr5.2:16996074-16996093	None:intergenic	30.0%
	TAGTAATGAGGAACATTACT+TGG	+	chr5.2:16997000-16997019	MS.gene82145:CDS	30.0%
	TATATGAATCAAAGGCAAAG+TGG	+	chr5.2:16997211-16997230	MS.gene82145:CDS	30.0%
	TCAAATCTTGATCTACTTTC+AGG	+	chr5.2:16996092-16996111	MS.gene82145:CDS	30.0%
	TCTCAAATACCAACCTTATT+TGG	+	chr5.2:16996583-16996602	MS.gene82145:CDS	30.0%
	TCTGTTACCTGAATTTGTAA+AGG	-	chr5.2:16996404-16996423	None:intergenic	30.0%
	TCTTGAAGAAAAGATAGTCT+CGG	+	chr5.2:16996364-16996383	MS.gene82145:CDS	30.0%
	TGAATTAGTAGATGCTTTGA+AGG	+	chr5.2:16997276-16997295	MS.gene82145:CDS	30.0%
	TGAATTTGTAAAGGGAAATC+TGG	-	chr5.2:16996395-16996414	None:intergenic	30.0%
	TTAATCACTGAAAATGGCTT+TGG	-	chr5.2:16996068-16996087	None:intergenic	30.0%
	TTGATTGGATTGAGTTTCAT+AGG	+	chr5.2:16997029-16997048	MS.gene82145:CDS	30.0%
!	CAAAGGTTTCTTTTATGGTT+CGG	-	chr5.2:16996551-16996570	None:intergenic	30.0%
!	CATATTTTGTATCTCTTCCA+TGG	-	chr5.2:16996630-16996649	None:intergenic	30.0%
!	TTCAAAGCATCTACTAATTC+AGG	-	chr5.2:16997277-16997296	None:intergenic	30.0%
!!	AAATAAGGTTGGTATTTGAG+AGG	-	chr5.2:16996584-16996603	None:intergenic	30.0%
!!	AATGTATTGTCTTTGGCTTT+CGG	+	chr5.2:16996899-16996918	MS.gene82145:CDS	30.0%
!!	GATTTGAAGTTACCTAGTTT+TGG	-	chr5.2:16996155-16996174	None:intergenic	30.0%
!!	GTGTTGGTTATATGAATCAA+AGG	+	chr5.2:16997203-16997222	MS.gene82145:CDS	30.0%
	AAGCTTTCTCTGCATATGTA+TGG	+	chr5.2:16996759-16996778	MS.gene82145:CDS	35.0%
	AATCAAAGGCAAAGTGGTAA+GGG	+	chr5.2:16997217-16997236	MS.gene82145:CDS	35.0%
	AATCATTACTATCCCTTTGC+TGG	+	chr5.2:16996215-16996234	MS.gene82145:CDS	35.0%
	AGCTTCAATCTTTGTTCTCT+TGG	-	chr5.2:16996744-16996763	None:intergenic	35.0%
	AGGATTCAAGAATTGAAGGA+AGG	+	chr5.2:16996929-16996948	MS.gene82145:CDS	35.0%
	CAAATCGTTTGTGACAACAA+TGG	+	chr5.2:16996272-16996291	MS.gene82145:CDS	35.0%
	CAGGTAACAGAATTTACTTG+TGG	+	chr5.2:16996413-16996432	MS.gene82145:CDS	35.0%
	CATCGGTAATGTATTGTCTT+TGG	+	chr5.2:16996892-16996911	MS.gene82145:CDS	35.0%
	GAAGGTTCATTATCAGACAT+AGG	+	chr5.2:16996947-16996966	MS.gene82145:CDS	35.0%
	GATTTGGAAGTCCTTTGTTA+GGG	+	chr5.2:16997152-16997171	MS.gene82145:CDS	35.0%
	GTAACAGAATTTACTTGTGG+TGG	+	chr5.2:16996416-16996435	MS.gene82145:CDS	35.0%
	TATACCTCAATTGAAACGGT+TGG	+	chr5.2:16997181-16997200	MS.gene82145:CDS	35.0%
	TTAGGTGATGCAACTTCATT+TGG	+	chr5.2:16996470-16996489	MS.gene82145:CDS	35.0%
	TTGTTGTCACAAACGATTTG+AGG	-	chr5.2:16996272-16996291	None:intergenic	35.0%
!!	AGTCAAGTTGATTTGGGATT+TGG	+	chr5.2:16997136-16997155	MS.gene82145:CDS	35.0%
!!	CCTGTTAGTCAAGTTGATTT+GGG	+	chr5.2:16997130-16997149	MS.gene82145:CDS	35.0%
!!	TCCTGTTAGTCAAGTTGATT+TGG	+	chr5.2:16997129-16997148	MS.gene82145:CDS	35.0%
	AACACCAACCGTTTCAATTG+AGG	-	chr5.2:16997188-16997207	None:intergenic	40.0%
	CCCAAATCAACTTGACTAAC+AGG	-	chr5.2:16997133-16997152	None:intergenic	40.0%
	GAATCAAAGGCAAAGTGGTA+AGG	+	chr5.2:16997216-16997235	MS.gene82145:CDS	40.0%
	GATGGAAGAGAAAGAATGAG+TGG	+	chr5.2:16996845-16996864	MS.gene82145:CDS	40.0%
	GCAGTCATGGGATGAAAAAT+TGG	-	chr5.2:16997307-16997326	None:intergenic	40.0%
	GGATTTGGAAGTCCTTTGTT+AGG	+	chr5.2:16997151-16997170	MS.gene82145:CDS	40.0%
	GGCAAGGATTCAAGAATTGA+AGG	+	chr5.2:16996925-16996944	MS.gene82145:CDS	40.0%
	GTATACACTGTCCCTAACAA+AGG	-	chr5.2:16997166-16997185	None:intergenic	40.0%
	TAGTGGTTTCTTCAGGACAA+AGG	+	chr5.2:16997104-16997123	MS.gene82145:CDS	40.0%
	TGAACAATGTGACCAGCAAA+GGG	-	chr5.2:16996230-16996249	None:intergenic	40.0%
	TGAAGATGAGAAGCAGTCAT+GGG	-	chr5.2:16997319-16997338	None:intergenic	40.0%
	TTGAACAATGTGACCAGCAA+AGG	-	chr5.2:16996231-16996250	None:intergenic	40.0%
!	CAGAGACTTGCTTCTGTTTA+TGG	+	chr5.2:16996716-16996735	MS.gene82145:CDS	40.0%
!	GTGCGCAAAGGTTTCTTTTA+TGG	-	chr5.2:16996556-16996575	None:intergenic	40.0%
!	TCAATTGAAACGGTTGGTGT+TGG	+	chr5.2:16997187-16997206	MS.gene82145:CDS	40.0%
!!	AAGATGGGTTGGTTAGTAGA+TGG	+	chr5.2:16996827-16996846	MS.gene82145:CDS	40.0%
!!	GGACTGTTTCTGCTATCTTA+TGG	+	chr5.2:16997251-16997270	MS.gene82145:CDS	40.0%
!!	TGGTTCGGTATGCATGATAA+CGG	-	chr5.2:16996536-16996555	None:intergenic	40.0%
	AGGTTTGATGCTTGCAAAGG+CGG	+	chr5.2:16997054-16997073	MS.gene82145:CDS	45.0%
	CCTGAAGAAACCACTAAGAC+AGG	-	chr5.2:16997100-16997119	None:intergenic	45.0%
	CCTGTCTTAGTGGTTTCTTC+AGG	+	chr5.2:16997097-16997116	MS.gene82145:CDS	45.0%
	CTGAAGATGAGAAGCAGTCA+TGG	-	chr5.2:16997320-16997339	None:intergenic	45.0%
	CTGCAGCATGTTAATACTCG+AGG	-	chr5.2:16996699-16996718	None:intergenic	45.0%
	TGGATTGAGTTTCATAGGCC+AGG	+	chr5.2:16997034-16997053	MS.gene82145:CDS	45.0%
!!	AGATGATGGACCTGTCTTAG+TGG	+	chr5.2:16997087-16997106	MS.gene82145:CDS	45.0%
!!	ATTGTCTTTGGCTTTCGGTG+AGG	+	chr5.2:16996904-16996923	MS.gene82145:CDS	45.0%
	AGGCAAAGTGGTAAGGGTGA+TGG	+	chr5.2:16997223-16997242	MS.gene82145:CDS	50.0%
	ATGCTTGCAAAGGCGGTGTT+AGG	+	chr5.2:16997061-16997080	MS.gene82145:CDS	50.0%
	GCCAGGTTTGATGCTTGCAA+AGG	+	chr5.2:16997051-16997070	MS.gene82145:CDS	50.0%
	GCCTTTGCAAGCATCAAACC+TGG	-	chr5.2:16997055-16997074	None:intergenic	50.0%
!	GCGGTGTTAGGACAAGATGA+TGG	+	chr5.2:16997073-16997092	MS.gene82145:CDS	50.0%
!	GTGGTAAGGGTGATGGTTCA+TGG	+	chr5.2:16997230-16997249	MS.gene82145:CDS	50.0%
!!	CTTTGGCTTTCGGTGAGGCA+AGG	+	chr5.2:16996909-16996928	MS.gene82145:CDS	55.0%
	TGAGAGGCGAACGTGCGCAA+AGG	-	chr5.2:16996568-16996587	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	16995969	16997345	16995969	ID=MS.gene82145
chr5.2	mRNA	16995969	16997345	16995969	ID=MS.gene82145.t1;Parent=MS.gene82145
chr5.2	exon	16995969	16997345	16995969	ID=MS.gene82145.t1.exon1;Parent=MS.gene82145.t1
chr5.2	CDS	16995969	16997345	16995969	ID=cds.MS.gene82145.t1;Parent=MS.gene82145.t1

Gene Sequence

>MS.gene82145

ATGTCACATATTCTCAATGATATTGCAGCCCAAGAATATGAAAAATTTGAAGTAAAGTTCATAAAGAAAAGTGTTGTAAAAGCTCTCAATCATTCACCAAAGCCATTTTCAGTGATTAATCTCTCAAATCTTGATCTACTTTCAGGCCGTTTTCCAGTTACATACTTGTATTTCTACAAAAAACCAAAACTAGGTAACTTCAAATCTTTTGTTAACAATCTTAAAACTTCTCTAGCACAAATACTCAATCATTACTATCCCTTTGCTGGTCACATTGTTCAAAATCATAAAACGGACGAACCTCAAATCGTTTGTGACAACAATGGTTCATTAGTCATTGAAGCTCAAGTAAATATTCCACTAAGTAATTTAGATTTTTATAATCTCAATGAAACTCTTGAAGAAAAGATAGTCTCGGTTGAACCAGATTTCCCTTTACAAATTCAGGTAACAGAATTTACTTGTGGTGGAATTTCAATAGCATTTACTTTTGATCATGCATTAGGTGATGCAACTTCATTTGGTAAATTCATTTCATCTTGGTGTGAAATAGCGCAAAATAAACCGTTATCATGCATACCGAACCATAAAAGAAACCTTTGCGCACGTTCGCCTCTCAAATACCAACCTTATTTGGATCAAATTTTTATCAAATGCACCATGGAAGAGATACAAAATATGAAAATAAATAAAAATATTTCGCTTAAACGTCTTTATCATATCGATGCCTCGAGTATTAACATGCTGCAGAGACTTGCTTCTGTTTATGGTACCAAGAGAACAAAGATTGAAGCTTTCTCTGCATATGTATGGAAGAAAATGATAAACACGGTTGATCAAAAACTTCATAAAAAATGCAAGATGGGTTGGTTAGTAGATGGAAGAGAAAGAATGAGTGGAAGCAAAAATTCAATGTCGAATTACATCGGTAATGTATTGTCTTTGGCTTTCGGTGAGGCAAGGATTCAAGAATTGAAGGAAGGTTCATTATCAGACATAGGTGAAATAGTTCATGATGCAATTTCAAAGGTTAGTAATGAGGAACATTACTTGGATTTGATTGATTGGATTGAGTTTCATAGGCCAGGTTTGATGCTTGCAAAGGCGGTGTTAGGACAAGATGATGGACCTGTCTTAGTGGTTTCTTCAGGACAAAGGTTTCCTGTTAGTCAAGTTGATTTGGGATTTGGAAGTCCTTTGTTAGGGACAGTGTATACCTCAATTGAAACGGTTGGTGTTGGTTATATGAATCAAAGGCAAAGTGGTAAGGGTGATGGTTCATGGACTGTTTCTGCTATCTTATGGCCTGAATTAGTAGATGCTTTGAAGGATGATCCAATTTTTCATCCCATGACTGCTTCTCATCTTCAGCTCTAG

Protein sequence

>MS.gene82145.t1

MSHILNDIAAQEYEKFEVKFIKKSVVKALNHSPKPFSVINLSNLDLLSGRFPVTYLYFYKKPKLGNFKSFVNNLKTSLAQILNHYYPFAGHIVQNHKTDEPQIVCDNNGSLVIEAQVNIPLSNLDFYNLNETLEEKIVSVEPDFPLQIQVTEFTCGGISIAFTFDHALGDATSFGKFISSWCEIAQNKPLSCIPNHKRNLCARSPLKYQPYLDQIFIKCTMEEIQNMKINKNISLKRLYHIDASSINMLQRLASVYGTKRTKIEAFSAYVWKKMINTVDQKLHKKCKMGWLVDGRERMSGSKNSMSNYIGNVLSLAFGEARIQELKEGSLSDIGEIVHDAISKVSNEEHYLDLIDWIEFHRPGLMLAKAVLGQDDGPVLVVSSGQRFPVSQVDLGFGSPLLGTVYTSIETVGVGYMNQRQSGKGDGSWTVSAILWPELVDALKDDPIFHPMTASHLQL