AlfalfaGEDB | MS.gene89994

M. sativa cultivar XinJiangDaYe / MS.gene89994

KEGG: K09228
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89994.t1	KEH17976.1	92.6	121	9	0	2	122	1	121	7.20E-58	233

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89994.t1	Q9LTH7	53.2	109	46	2	18	122	14	121	7.4e-27	121.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89994.t1	G7LCM1	92.6	121	9	0	2	122	1	121	5.2e-58	233.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049346	MS.gene89994	0.830743	2.47E-55	-1.69E-46
MS.gene049427	MS.gene89994	0.805068	1.60E-49	-1.69E-46
MS.gene049661	MS.gene89994	0.800929	1.14E-48	-1.69E-46
MS.gene050060	MS.gene89994	0.801655	8.13E-49	-1.69E-46
MS.gene050301	MS.gene89994	0.842891	1.96E-58	-1.69E-46
MS.gene050397	MS.gene89994	0.811836	5.73E-51	-1.69E-46
MS.gene050404	MS.gene89994	0.852111	5.72E-61	-1.69E-46
MS.gene050406	MS.gene89994	0.862933	3.57E-64	-1.69E-46
MS.gene050670	MS.gene89994	0.805935	1.05E-49	-1.69E-46
MS.gene05101	MS.gene89994	0.817137	3.85E-52	-1.69E-46
MS.gene051471	MS.gene89994	0.82527	5.14E-54	-1.69E-46
MS.gene051582	MS.gene89994	0.814429	1.55E-51	-1.69E-46
MS.gene052135	MS.gene89994	0.808714	2.70E-50	-1.69E-46
MS.gene052188	MS.gene89994	0.844687	6.48E-59	-1.69E-46
MS.gene052253	MS.gene89994	0.834497	2.90E-56	-1.69E-46
MS.gene052310	MS.gene89994	0.85015	2.05E-60	-1.69E-46
MS.gene052340	MS.gene89994	0.886933	2.22E-72	-1.69E-46
MS.gene052485	MS.gene89994	0.802474	5.52E-49	-1.69E-46
MS.gene053073	MS.gene89994	0.837129	6.23E-57	-1.69E-46
MS.gene053557	MS.gene89994	0.844989	5.37E-59	-1.69E-46
MS.gene053836	MS.gene89994	0.819523	1.11E-52	-1.69E-46
MS.gene054123	MS.gene89994	0.844404	7.72E-59	-1.69E-46
MS.gene054349	MS.gene89994	0.806881	6.63E-50	-1.69E-46
MS.gene055116	MS.gene89994	0.855717	5.23E-62	-1.69E-46
MS.gene05553	MS.gene89994	0.853782	1.90E-61	-1.69E-46
MS.gene056609	MS.gene89994	0.826145	3.19E-54	-1.69E-46
MS.gene056612	MS.gene89994	0.833993	3.87E-56	-1.69E-46
MS.gene056659	MS.gene89994	0.828177	1.04E-54	-1.69E-46
MS.gene056719	MS.gene89994	0.818565	1.83E-52	-1.69E-46
MS.gene056897	MS.gene89994	0.810035	1.41E-50	-1.69E-46
MS.gene05702	MS.gene89994	0.847559	1.07E-59	-1.69E-46
MS.gene057124	MS.gene89994	0.803265	3.79E-49	-1.69E-46
MS.gene05733	MS.gene89994	0.800426	1.45E-48	-1.69E-46
MS.gene057396	MS.gene89994	0.825162	5.45E-54	-1.69E-46
MS.gene057449	MS.gene89994	0.800481	1.41E-48	-1.69E-46
MS.gene057450	MS.gene89994	0.809189	2.14E-50	-1.69E-46
MS.gene058002	MS.gene89994	0.825821	3.80E-54	-1.69E-46
MS.gene058007	MS.gene89994	0.820546	6.48E-53	-1.69E-46
MS.gene058142	MS.gene89994	0.836977	6.82E-57	-1.69E-46
MS.gene058233	MS.gene89994	0.808208	3.47E-50	-1.69E-46
MS.gene058276	MS.gene89994	0.814963	1.18E-51	-1.69E-46
MS.gene058365	MS.gene89994	0.804477	2.12E-49	-1.69E-46
MS.gene058660	MS.gene89994	0.807284	5.45E-50	-1.69E-46
MS.gene058928	MS.gene89994	0.81045	1.15E-50	-1.69E-46
MS.gene059011	MS.gene89994	0.813688	2.25E-51	-1.69E-46
MS.gene05907	MS.gene89994	0.831087	2.04E-55	-1.69E-46
MS.gene059285	MS.gene89994	0.801903	7.23E-49	-1.69E-46
MS.gene059597	MS.gene89994	0.83875	2.39E-57	-1.69E-46
MS.gene059676	MS.gene89994	0.815385	9.50E-52	-1.69E-46
MS.gene059688	MS.gene89994	0.84258	2.37E-58	-1.69E-46
MS.gene059692	MS.gene89994	0.856467	3.16E-62	-1.69E-46
MS.gene059695	MS.gene89994	0.853793	1.89E-61	-1.69E-46
MS.gene05984	MS.gene89994	0.807759	4.32E-50	-1.69E-46
MS.gene059894	MS.gene89994	0.865084	7.63E-65	-1.69E-46
MS.gene060247	MS.gene89994	0.816951	4.24E-52	-1.69E-46
MS.gene060407	MS.gene89994	0.865437	5.91E-65	-1.69E-46
MS.gene061049	MS.gene89994	0.868234	7.60E-66	-1.69E-46
MS.gene061051	MS.gene89994	-0.824624	7.30E-54	-1.69E-46
MS.gene061159	MS.gene89994	0.823737	1.18E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTATTATCAATATCTTTA+AGG	0.122806	8.3:-79886537	None:intergenic
CCTCGTATTTGTCTGGTTGA+TGG	0.287516	8.3:-79886477	None:intergenic
GTTAGGTTTGGTTGTATCAA+TGG	0.379115	8.3:-79886353	None:intergenic
TTGGCTTCCTCGTATTTGTC+TGG	0.410058	8.3:-79886484	None:intergenic
ACAATTCCTTGATGAATACT+TGG	0.419418	8.3:-79886562	None:intergenic
TCAAGGAAGCATGTGAAACT+TGG	0.431792	8.3:+79886593	MS.gene89994:CDS
TTGAATGTGGAATCAAGGTT+AGG	0.444552	8.3:-79886370	None:intergenic
GTTCTTGAGGAGGTGAAAGA+TGG	0.464397	8.3:+79886655	MS.gene89994:CDS
GTTCATTGAATGTGGAATCA+AGG	0.469424	8.3:-79886375	None:intergenic
TTCTACTCATTCAAGAAGCA+TGG	0.481021	8.3:+79886327	None:intergenic
AATAACATGACTTGTGTTGT+TGG	0.488088	8.3:-79886503	None:intergenic
TGTGGAATCAAGGTTAGGTT+TGG	0.492547	8.3:-79886365	None:intergenic
CCCTTTGAGTGTTCTTGAGG+AGG	0.493576	8.3:+79886645	MS.gene89994:CDS
CAAGGAAGCATGTGAAACTT+GGG	0.494320	8.3:+79886594	MS.gene89994:CDS
AGGAATTGTTGACGATATCA+AGG	0.530296	8.3:+79886576	MS.gene89994:CDS
GAAACAAAAGCAGGTGTGAA+AGG	0.532657	8.3:+79886412	MS.gene89994:CDS
TGCTTTGCGTTCATTGAATG+TGG	0.544374	8.3:-79886383	None:intergenic
TCTACTCATTCAAGAAGCAT+GGG	0.545841	8.3:+79886328	None:intergenic
AATCCCTTTGAGTGTTCTTG+AGG	0.545874	8.3:+79886642	MS.gene89994:CDS
ACCTCCTCAAGAACACTCAA+AGG	0.547379	8.3:-79886646	None:intergenic
CCTCCTCAAGAACACTCAAA+GGG	0.551009	8.3:-79886645	None:intergenic
AGGTGAAAGATGGAGTGAAG+AGG	0.558342	8.3:+79886665	MS.gene89994:CDS
CGTATTTGTCTGGTTGATGG+TGG	0.562970	8.3:-79886474	None:intergenic
CAATTCCTTGATGAATACTT+GGG	0.576394	8.3:-79886561	None:intergenic
ATATCTTTAAGGTCGATGAC+AGG	0.590653	8.3:-79886526	None:intergenic
GTGAAAGGACTTGTTGATGC+AGG	0.590786	8.3:+79886427	MS.gene89994:CDS
GCATTTGATGAAACAAAAGC+AGG	0.595830	8.3:+79886403	MS.gene89994:CDS
AAAGACCCAAGTATTCATCA+AGG	0.600194	8.3:+79886556	MS.gene89994:CDS
GGTTGATGGTGGAATAAGCT+TGG	0.607514	8.3:-79886463	None:intergenic
AAGGAAGCATGTGAAACTTG+GGG	0.628246	8.3:+79886595	MS.gene89994:CDS
CCATCAACCAGACAAATACG+AGG	0.724113	8.3:+79886477	MS.gene89994:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTATTATCAATATCTTTA+AGG	-	chr8.3:79886540-79886559	None:intergenic	15.0%
!!!	TTTTTTCAAGTTGTTAATCA+TGG	+	chr8.3:79886619-79886638	MS.gene89994:CDS	20.0%
	ACAATTCCTTGATGAATACT+TGG	-	chr8.3:79886565-79886584	None:intergenic	30.0%
	CAATTCCTTGATGAATACTT+GGG	-	chr8.3:79886564-79886583	None:intergenic	30.0%
!	AATAACATGACTTGTGTTGT+TGG	-	chr8.3:79886506-79886525	None:intergenic	30.0%
	AAAGACCCAAGTATTCATCA+AGG	+	chr8.3:79886556-79886575	MS.gene89994:CDS	35.0%
	AGGAATTGTTGACGATATCA+AGG	+	chr8.3:79886576-79886595	MS.gene89994:CDS	35.0%
	GCATTTGATGAAACAAAAGC+AGG	+	chr8.3:79886403-79886422	MS.gene89994:CDS	35.0%
	GTTCATTGAATGTGGAATCA+AGG	-	chr8.3:79886378-79886397	None:intergenic	35.0%
	TTGAATGTGGAATCAAGGTT+AGG	-	chr8.3:79886373-79886392	None:intergenic	35.0%
!	GTTAGGTTTGGTTGTATCAA+TGG	-	chr8.3:79886356-79886375	None:intergenic	35.0%
!!	ATATCTTTAAGGTCGATGAC+AGG	-	chr8.3:79886529-79886548	None:intergenic	35.0%
	AATCCCTTTGAGTGTTCTTG+AGG	+	chr8.3:79886642-79886661	MS.gene89994:CDS	40.0%
	GAAACAAAAGCAGGTGTGAA+AGG	+	chr8.3:79886412-79886431	MS.gene89994:CDS	40.0%
	TGCTTTGCGTTCATTGAATG+TGG	-	chr8.3:79886386-79886405	None:intergenic	40.0%
	TGTGGAATCAAGGTTAGGTT+TGG	-	chr8.3:79886368-79886387	None:intergenic	40.0%
!	AAGGAAGCATGTGAAACTTG+GGG	+	chr8.3:79886595-79886614	MS.gene89994:CDS	40.0%
!	CAAGGAAGCATGTGAAACTT+GGG	+	chr8.3:79886594-79886613	MS.gene89994:CDS	40.0%
!	TCAAGGAAGCATGTGAAACT+TGG	+	chr8.3:79886593-79886612	MS.gene89994:CDS	40.0%
	ACCTCCTCAAGAACACTCAA+AGG	-	chr8.3:79886649-79886668	None:intergenic	45.0%
	AGGTGAAAGATGGAGTGAAG+AGG	+	chr8.3:79886665-79886684	MS.gene89994:CDS	45.0%
	CCATCAACCAGACAAATACG+AGG	+	chr8.3:79886477-79886496	MS.gene89994:CDS	45.0%
	CCTCCTCAAGAACACTCAAA+GGG	-	chr8.3:79886648-79886667	None:intergenic	45.0%
	CCTCGTATTTGTCTGGTTGA+TGG	-	chr8.3:79886480-79886499	None:intergenic	45.0%
	GTTCTTGAGGAGGTGAAAGA+TGG	+	chr8.3:79886655-79886674	MS.gene89994:CDS	45.0%
	TTGGCTTCCTCGTATTTGTC+TGG	-	chr8.3:79886487-79886506	None:intergenic	45.0%
!!	CGTATTTGTCTGGTTGATGG+TGG	-	chr8.3:79886477-79886496	None:intergenic	45.0%
!!	GGTTGATGGTGGAATAAGCT+TGG	-	chr8.3:79886466-79886485	None:intergenic	45.0%
!!	GTGAAAGGACTTGTTGATGC+AGG	+	chr8.3:79886427-79886446	MS.gene89994:CDS	45.0%
	CCCTTTGAGTGTTCTTGAGG+AGG	+	chr8.3:79886645-79886664	MS.gene89994:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	79886342	79886708	79886342	ID=MS.gene89994
chr8.3	mRNA	79886342	79886708	79886342	ID=MS.gene89994.t1;Parent=MS.gene89994
chr8.3	exon	79886342	79886708	79886342	ID=MS.gene89994.t1.exon1;Parent=MS.gene89994.t1
chr8.3	CDS	79886342	79886708	79886342	ID=cds.MS.gene89994.t1;Parent=MS.gene89994.t1

Gene Sequence	>MS.gene89994 AAGCATGGGAACCATTGATACAACCAAACCTAACCTTGATTCCACATTCAATGAACGCAAAGCATTTGATGAAACAAAAGCAGGTGTGAAAGGACTTGTTGATGCAGGAGTTGAGAAGATACCAAGCTTATTCCACCATCAACCAGACAAATACGAGGAAGCCAACAACACAAGTCATGTTATTCCTGTCATCGACCTTAAAGATATTGATAATAAAGACCCAAGTATTCATCAAGGAATTGTTGACGATATCAAGGAAGCATGTGAAACTTGGGGTTTTTTTCAAGTTGTTAATCATGGAATCCCTTTGAGTGTTCTTGAGGAGGTGAAAGATGGAGTGAAGAGGTTTTATGAACAAGACACAGAA
Protein sequence	>MS.gene89994.t1 KHGNHUYNQTUPUFHIQUTQSIUUNKSRCERTCUCRSUEDTKLIPPSTRQIRGSQQHKSCYSCHRPURYUUURPKYSSRNCURYQGSMUNLGFFSSCUSWNPFECSUGGERWSEEVLUTRHR