Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96983.t1 | XP_013449911.1 | 100 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.70E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96983.t1 | Q93XX8 | 79.7 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 2.6e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96983.t1 | A0A072U2M3 | 100.0 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.7e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene96983 | MS.gene033017 | PPI |
| MS.gene96983 | MS.gene067809 | PPI |
| MS.gene96983 | MS.gene31515 | PPI |
| MS.gene96983 | MS.gene41916 | PPI |
| MS.gene96983 | MS.gene45344 | PPI |
| MS.gene96983 | MS.gene028023 | PPI |
| MS.gene96983 | MS.gene98436 | PPI |
| MS.gene95758 | MS.gene96983 | PPI |
| MS.gene96983 | MS.gene99744 | PPI |
| MS.gene96983 | MS.gene003501 | PPI |
| MS.gene059422 | MS.gene96983 | PPI |
| MS.gene96983 | MS.gene001986 | PPI |
| MS.gene96983 | MS.gene029242 | PPI |
| MS.gene96983 | MS.gene08205 | PPI |
| MS.gene96983 | MS.gene90954 | PPI |
| MS.gene96983 | MS.gene038800 | PPI |
| MS.gene96983 | MS.gene000714 | PPI |
| MS.gene96983 | MS.gene51868 | PPI |
| MS.gene96983 | MS.gene68979 | PPI |
| MS.gene96983 | MS.gene054186 | PPI |
| MS.gene96983 | MS.gene056257 | PPI |
| MS.gene96983 | MS.gene72894 | PPI |
| MS.gene96983 | MS.gene044498 | PPI |
| MS.gene96983 | MS.gene90957 | PPI |
| MS.gene96983 | MS.gene52097 | PPI |
| MS.gene96983 | MS.gene037324 | PPI |
| MS.gene064180 | MS.gene96983 | PPI |
| MS.gene96983 | MS.gene046040 | PPI |
| MS.gene96983 | MS.gene057730 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96983.t1 | MTR_7g097110 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 2.26e-43 | 134 |
| MS.gene96983.t1 | MTR_8g069770 | 85.938 | 64 | 9 | 0 | 1 | 64 | 1 | 64 | 7.70e-36 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96983.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene96983.t1 | AT2G20490 | 79.688 | 64 | 13 | 0 | 1 | 64 | 1 | 64 | 8.91e-33 | 107 |
| MS.gene96983.t1 | AT2G20490 | 78.125 | 64 | 13 | 1 | 1 | 64 | 1 | 63 | 2.46e-30 | 101 |
Find 16 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATACTTTGGAGCTGGATGC+TGG | 0.320451 | 7.4:-12498042 | None:intergenic |
| TGCTCTGAAAATTTGTCATC+AGG | 0.402146 | 7.4:-12497980 | None:intergenic |
| ATTTGTCATCAGGTGAAAAC+CGG | 0.402459 | 7.4:-12497970 | None:intergenic |
| TTGAATATAGCTCAATACTT+TGG | 0.457998 | 7.4:-12498055 | None:intergenic |
| GCCACTCAATCTGCTCATCC+AGG | 0.502158 | 7.4:+12496661 | MS.gene96983:CDS |
| GTTAAACTGAAGAAGCGATT+CGG | 0.503962 | 7.4:+12498007 | MS.gene96983:CDS |
| TGCAGAAAGAGTCACCGCTT+GGG | 0.506313 | 7.4:+12496635 | MS.gene96983:intron |
| TTTGGAGCTGGATGCTGGGT+TGG | 0.510089 | 7.4:-12498037 | None:intergenic |
| ATACTTTGGAGCTGGATGCT+GGG | 0.511217 | 7.4:-12498041 | None:intergenic |
| ATAGCTCAATACTTTGGAGC+TGG | 0.511390 | 7.4:-12498049 | None:intergenic |
| TTGCAGAAAGAGTCACCGCT+TGG | 0.526675 | 7.4:+12496634 | MS.gene96983:intron |
| TGACAAAGTCTACACTACTA+AGG | 0.530304 | 7.4:+12496487 | MS.gene96983:CDS |
| TTTGTCATCAGGTGAAAACC+GGG | 0.561589 | 7.4:-12497969 | None:intergenic |
| ACCTGGATGAGCAGATTGAG+TGG | 0.614681 | 7.4:-12496662 | None:intergenic |
| GATGTAAAACTGAAGATACA+TGG | 0.619474 | 7.4:-12496453 | None:intergenic |
| GATTGAGTGGCTAACCCAAG+CGG | 0.715814 | 7.4:-12496649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTATATTTTTTAAAAA+AGG | + | chr7.4:12497377-12497396 | MS.gene96983:intron | 0.0% |
| !! | AAAAAATAATTAACAGTTGT+AGG | - | chr7.4:12497927-12497946 | None:intergenic | 15.0% |
| !! | AAAAGTTGTCAAAATAAAAA+AGG | - | chr7.4:12497856-12497875 | None:intergenic | 15.0% |
| !! | TAAAGAAAAATTACTTGATT+TGG | + | chr7.4:12497650-12497669 | MS.gene96983:intron | 15.0% |
| !! | AACAACAAAAGAATTAGATT+AGG | - | chr7.4:12497624-12497643 | None:intergenic | 20.0% |
| !! | CTAAAAAAAAAATGAGACAA+AGG | + | chr7.4:12497711-12497730 | MS.gene96983:intron | 20.0% |
| !!! | CAATATTTTACCTCAAAAAT+TGG | - | chr7.4:12496860-12496879 | None:intergenic | 20.0% |
| !!! | CATTTTTTTTTTAGCAAAGA+AGG | - | chr7.4:12497705-12497724 | None:intergenic | 20.0% |
| ! | AAATATTGAAACCGATAGAT+AGG | + | chr7.4:12496872-12496891 | MS.gene96983:intron | 25.0% |
| ! | AATTAACAGTTGTAGGAAAT+TGG | - | chr7.4:12497920-12497939 | None:intergenic | 25.0% |
| ! | AGGTAAGTAAAGAAATTGAT+TGG | - | chr7.4:12497307-12497326 | None:intergenic | 25.0% |
| ! | ATCAATTTCTTTACTTACCT+TGG | + | chr7.4:12497307-12497326 | MS.gene96983:intron | 25.0% |
| ! | TCAAAGATTAAAGCACAAAA+TGG | - | chr7.4:12496717-12496736 | None:intergenic | 25.0% |
| ! | TCTGTGTCATATGAAAATTT+AGG | + | chr7.4:12497068-12497087 | MS.gene96983:intron | 25.0% |
| ! | TTAAAAGTAGAAAAAACGTG+AGG | + | chr7.4:12496979-12496998 | MS.gene96983:intron | 25.0% |
| !! | ATTTTACCTCAAAAATTGGA+TGG | - | chr7.4:12496856-12496875 | None:intergenic | 25.0% |
| !! | TTTAATCTTTGAAGAAGCAT+GGG | + | chr7.4:12496725-12496744 | MS.gene96983:intron | 25.0% |
| !! | TTTTACAAATGCTGTTCAAT+AGG | + | chr7.4:12497872-12497891 | MS.gene96983:intron | 25.0% |
| !! | TTTTACCTCAAAAATTGGAT+GGG | - | chr7.4:12496855-12496874 | None:intergenic | 25.0% |
| !!! | ATGTTTTGAATTGTGAAGTA+AGG | + | chr7.4:12497744-12497763 | MS.gene96983:intron | 25.0% |
| !!! | CAATACAAGTTGATAAGTAT+TGG | - | chr7.4:12497406-12497425 | None:intergenic | 25.0% |
| !!! | TCCAAGTTAAATTGTTTTAG+CGG | - | chr7.4:12497557-12497576 | None:intergenic | 25.0% |
| !!! | TGTTTTGAATTGTGAAGTAA+GGG | + | chr7.4:12497745-12497764 | MS.gene96983:intron | 25.0% |
| AAATTCATCTCTTTCAAACC+TGG | + | chr7.4:12496512-12496531 | MS.gene96983:intron | 30.0% | |
| AATTCATCTCTTTCAAACCT+GGG | + | chr7.4:12496513-12496532 | MS.gene96983:intron | 30.0% | |
| ACAAAATGGCAGAAAAACTA+TGG | - | chr7.4:12496703-12496722 | None:intergenic | 30.0% | |
| ACCGCTAAAACAATTTAACT+TGG | + | chr7.4:12497553-12497572 | MS.gene96983:intron | 30.0% | |
| AGAAACTTTGGATTTGCAAT+TGG | + | chr7.4:12496901-12496920 | MS.gene96983:intron | 30.0% | |
| AGTGGATCATGAAAAAGAAT+AGG | - | chr7.4:12497095-12497114 | None:intergenic | 30.0% | |
| GGATTTGCAATTGGATATAT+GGG | + | chr7.4:12496910-12496929 | MS.gene96983:intron | 30.0% | |
| TGGATTTGCAATTGGATATA+TGG | + | chr7.4:12496909-12496928 | MS.gene96983:intron | 30.0% | |
| ! | CAGTTTTACATCAACGATAA+CGG | + | chr7.4:12496464-12496483 | MS.gene96983:CDS | 30.0% |
| ! | CTTTAATCTTTGAAGAAGCA+TGG | + | chr7.4:12496724-12496743 | MS.gene96983:intron | 30.0% |
| ! | GTTTACATCTGATGCATTTT+CGG | + | chr7.4:12497578-12497597 | MS.gene96983:intron | 30.0% |
| !! | GCTAAAACAATTTAACTTGG+AGG | + | chr7.4:12497556-12497575 | MS.gene96983:intron | 30.0% |
| !! | TTTGGATTTGCTATCGAAAA+CGG | - | chr7.4:12497271-12497290 | None:intergenic | 30.0% |
| !!! | GCATTATTTTGATTCTGCAT+TGG | + | chr7.4:12497802-12497821 | MS.gene96983:intron | 30.0% |
| !!! | GTGATTTTGGTTACAACATT+AGG | + | chr7.4:12497488-12497507 | MS.gene96983:intron | 30.0% |
| AAAAATGAGACAAAGGTCTG+AGG | + | chr7.4:12497718-12497737 | MS.gene96983:intron | 35.0% | |
| AGTTGTAGGAAATTGGATCA+TGG | - | chr7.4:12497913-12497932 | None:intergenic | 35.0% | |
| ATTGGTCGTGTTAGAAACTT+TGG | - | chr7.4:12497289-12497308 | None:intergenic | 35.0% | |
| ATTTGTCATCAGGTGAAAAC+CGG | - | chr7.4:12497973-12497992 | None:intergenic | 35.0% | |
| GAGATACGTTAAACATGAAG+AGG | - | chr7.4:12497174-12497193 | None:intergenic | 35.0% | |
| GTTAAACTGAAGAAGCGATT+CGG | + | chr7.4:12498007-12498026 | MS.gene96983:CDS | 35.0% | |
| GTTGTAACCAAAATCACTGT+GGG | - | chr7.4:12497485-12497504 | None:intergenic | 35.0% | |
| GTTGTAGGAAATTGGATCAT+GGG | - | chr7.4:12497912-12497931 | None:intergenic | 35.0% | |
| TATTACAATCCTTTGATCCC+AGG | - | chr7.4:12496533-12496552 | None:intergenic | 35.0% | |
| TCTTTACTTACCTTGGAGAT+GGG | + | chr7.4:12497314-12497333 | MS.gene96983:intron | 35.0% | |
| TGACAAAGTCTACACTACTA+AGG | + | chr7.4:12496487-12496506 | MS.gene96983:CDS | 35.0% | |
| TGTTGTAACCAAAATCACTG+TGG | - | chr7.4:12497486-12497505 | None:intergenic | 35.0% | |
| TTCTTTACTTACCTTGGAGA+TGG | + | chr7.4:12497313-12497332 | MS.gene96983:intron | 35.0% | |
| TTGTAACCAAAATCACTGTG+GGG | - | chr7.4:12497484-12497503 | None:intergenic | 35.0% | |
| TTTAGCAAAGAAGGATAGAG+AGG | - | chr7.4:12497696-12497715 | None:intergenic | 35.0% | |
| TTTGCAATGAGAATCAAGAG+TGG | - | chr7.4:12497138-12497157 | None:intergenic | 35.0% | |
| TTTGCAATTGGATATATGGG+AGG | + | chr7.4:12496913-12496932 | MS.gene96983:intron | 35.0% | |
| ! | ATCTGATGCATTTTCGGTAT+TGG | + | chr7.4:12497584-12497603 | MS.gene96983:intron | 35.0% |
| ! | TCTGATGCATTTTCGGTATT+GGG | + | chr7.4:12497585-12497604 | MS.gene96983:intron | 35.0% |
| !! | GGTGATTTTAGCAAAATACC+TGG | - | chr7.4:12496682-12496701 | None:intergenic | 35.0% |
| !! | TGCTCTGAAAATTTGTCATC+AGG | - | chr7.4:12497983-12498002 | None:intergenic | 35.0% |
| !! | TTGGATTTGCTATCGAAAAC+GGG | - | chr7.4:12497270-12497289 | None:intergenic | 35.0% |
| !! | TTTCTAGCTGACCTATCTAT+CGG | - | chr7.4:12496886-12496905 | None:intergenic | 35.0% |
| CTTTACTTACCTTGGAGATG+GGG | + | chr7.4:12497315-12497334 | MS.gene96983:intron | 40.0% | |
| GATAGGTCAGCTAGAAACTT+TGG | + | chr7.4:12496889-12496908 | MS.gene96983:intron | 40.0% | |
| GGGGAAAAGGTCTTTAAACA+TGG | - | chr7.4:12497465-12497484 | None:intergenic | 40.0% | |
| GTCGCGATTATAATTGAGAG+CGG | + | chr7.4:12497019-12497038 | MS.gene96983:intron | 40.0% | |
| TCTTTCAAACCTGGGATCAA+AGG | + | chr7.4:12496521-12496540 | MS.gene96983:intron | 40.0% | |
| TTTGTCATCAGGTGAAAACC+GGG | - | chr7.4:12497972-12497991 | None:intergenic | 40.0% | |
| ! | AAACATGGACGCAAGAGTTT+TGG | - | chr7.4:12497450-12497469 | None:intergenic | 40.0% |
| ! | CTGATGCATTTTCGGTATTG+GGG | + | chr7.4:12497586-12497605 | MS.gene96983:intron | 40.0% |
| !! | TGGATTTGCTATCGAAAACG+GGG | - | chr7.4:12497269-12497288 | None:intergenic | 40.0% |
| CCAAAATCACTGTGGGGAAA+AGG | - | chr7.4:12497478-12497497 | None:intergenic | 45.0% | |
| CCTCAAAAATTGGATGGGTG+TGG | - | chr7.4:12496850-12496869 | None:intergenic | 45.0% | |
| GCGCATTTGAGAATTGAGAG+TGG | - | chr7.4:12497113-12497132 | None:intergenic | 45.0% | |
| ! | CCACACCCATCCAATTTTTG+AGG | + | chr7.4:12496847-12496866 | MS.gene96983:intron | 45.0% |
| ! | CCTTTTCCCCACAGTGATTT+TGG | + | chr7.4:12497475-12497494 | MS.gene96983:intron | 45.0% |
| ! | TGTTTTCCAATACTGCAGCC+CGG | + | chr7.4:12497951-12497970 | MS.gene96983:intron | 45.0% |
| !! | AATACTTTGGAGCTGGATGC+TGG | - | chr7.4:12498045-12498064 | None:intergenic | 45.0% |
| !! | ATACTTTGGAGCTGGATGCT+GGG | - | chr7.4:12498044-12498063 | None:intergenic | 45.0% |
| !! | GGATTTGCTATCGAAAACGG+GGG | - | chr7.4:12497268-12497287 | None:intergenic | 45.0% |
| ACCTGGATGAGCAGATTGAG+TGG | - | chr7.4:12496665-12496684 | None:intergenic | 50.0% | |
| GATTGAGTGGCTAACCCAAG+CGG | - | chr7.4:12496652-12496671 | None:intergenic | 50.0% | |
| TGAAAACCGGGCTGCAGTAT+TGG | - | chr7.4:12497960-12497979 | None:intergenic | 50.0% | |
| TGCAGAAAGAGTCACCGCTT+GGG | + | chr7.4:12496635-12496654 | MS.gene96983:intron | 50.0% | |
| TTGCAGAAAGAGTCACCGCT+TGG | + | chr7.4:12496634-12496653 | MS.gene96983:intron | 50.0% | |
| TTGCTATCGAAAACGGGGGT+GGG | - | chr7.4:12497264-12497283 | None:intergenic | 50.0% | |
| ! | TTTGCTATCGAAAACGGGGG+TGG | - | chr7.4:12497265-12497284 | None:intergenic | 50.0% |
| GCCACTCAATCTGCTCATCC+AGG | + | chr7.4:12496661-12496680 | MS.gene96983:CDS | 55.0% | |
| TGCTATCGAAAACGGGGGTG+GGG | - | chr7.4:12497263-12497282 | None:intergenic | 55.0% | |
| ! | TTTGGAGCTGGATGCTGGGT+TGG | - | chr7.4:12498040-12498059 | None:intergenic | 55.0% |
| CTATCGAAAACGGGGGTGGG+GGG | - | chr7.4:12497261-12497280 | None:intergenic | 60.0% | |
| GCTATCGAAAACGGGGGTGG+GGG | - | chr7.4:12497262-12497281 | None:intergenic | 60.0% | |
| CAACGCGTGCCCCATCTCCA+AGG | - | chr7.4:12497327-12497346 | None:intergenic | 65.0% | |
| GGAGATGGGGCACGCGTTGA+TGG | + | chr7.4:12497328-12497347 | MS.gene96983:intron | 65.0% | |
| TCGAAAACGGGGGTGGGGGG+TGG | - | chr7.4:12497258-12497277 | None:intergenic | 70.0% | |
| AACGGGGGTGGGGGGTGGTG+AGG | - | chr7.4:12497253-12497272 | None:intergenic | 75.0% | |
| ACGGGGGTGGGGGGTGGTGA+GGG | - | chr7.4:12497252-12497271 | None:intergenic | 75.0% | |
| GGTGGGGGGTGGTGAGGGGT+GGG | - | chr7.4:12497247-12497266 | None:intergenic | 75.0% | |
| GTGGGGGGTGGTGAGGGGTG+GGG | - | chr7.4:12497246-12497265 | None:intergenic | 75.0% | |
| TGGGGGGTGGTGAGGGGTGG+GGG | - | chr7.4:12497245-12497264 | None:intergenic | 75.0% | |
| ! | CGGGGGTGGGGGGTGGTGAG+GGG | - | chr7.4:12497251-12497270 | None:intergenic | 80.0% |
| ! | GGGGGGTGGTGAGGGGTGGG+GGG | - | chr7.4:12497244-12497263 | None:intergenic | 80.0% |
| ! | GGGTGGGGGGTGGTGAGGGG+TGG | - | chr7.4:12497248-12497267 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 12496455 | 12498066 | 12496455 | ID=MS.gene96983 |
| chr7.4 | mRNA | 12496455 | 12498066 | 12496455 | ID=MS.gene96983.t1;Parent=MS.gene96983 |
| chr7.4 | exon | 12496455 | 12496508 | 12496455 | ID=MS.gene96983.t1.exon1;Parent=MS.gene96983.t1 |
| chr7.4 | CDS | 12496455 | 12496508 | 12496455 | ID=cds.MS.gene96983.t1;Parent=MS.gene96983.t1 |
| chr7.4 | exon | 12496640 | 12496682 | 12496640 | ID=MS.gene96983.t1.exon2;Parent=MS.gene96983.t1 |
| chr7.4 | CDS | 12496640 | 12496682 | 12496640 | ID=cds.MS.gene96983.t1;Parent=MS.gene96983.t1 |
| chr7.4 | exon | 12497969 | 12498066 | 12497969 | ID=MS.gene96983.t1.exon3;Parent=MS.gene96983.t1 |
| chr7.4 | CDS | 12497969 | 12498066 | 12497969 | ID=cds.MS.gene96983.t1;Parent=MS.gene96983.t1 |
| Gene Sequence |
| Protein sequence |
