AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97477


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97477.t1 MTR_7g056337 100.000 153 0 0 1 153 63 215 2.29e-112 317
MS.gene97477.t1 MTR_8g087430 71.242 153 44 0 1 153 63 215 6.33e-79 233
MS.gene97477.t1 MTR_4g075550 34.839 155 86 5 12 153 117 269 2.14e-22 90.1
MS.gene97477.t1 MTR_3g113920 34.783 161 90 5 6 153 111 269 9.68e-22 88.6
MS.gene97477.t1 MTR_8g012615 33.129 163 94 3 1 150 40 200 6.22e-19 79.7
MS.gene97477.t1 MTR_8g012595 31.579 152 88 3 12 149 118 267 3.51e-16 73.6
MS.gene97477.t1 MTR_8g012605 36.036 111 64 3 1 107 107 214 2.91e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97477.t1 AT3G25040 85.621 153 22 0 1 153 63 215 9.09e-98 281
MS.gene97477.t1 AT1G29330 70.588 153 45 0 1 153 1 153 1.15e-79 233
MS.gene97477.t1 AT1G29330 70.588 153 45 0 1 153 63 215 1.34e-79 234
MS.gene97477.t1 AT1G75760 34.783 161 90 5 6 153 111 269 2.04e-22 90.1
MS.gene97477.t1 AT1G19970 35.484 155 85 5 12 153 117 269 5.52e-22 89.0
MS.gene97477.t1 AT4G38790 33.735 166 95 5 1 153 107 270 2.28e-21 87.4
MS.gene97477.t1 AT1G75760 33.784 148 84 4 6 141 111 256 1.07e-20 85.5
MS.gene97477.t1 AT3G25160 33.333 156 87 5 6 147 113 265 9.16e-20 83.2
MS.gene97477.t1 AT2G21190 31.928 166 98 5 1 153 103 266 1.45e-19 82.8
MS.gene97477.t1 AT1G75760 35.238 105 63 2 6 107 111 213 7.16e-17 74.7
MS.gene97477.t1 AT1G19970 36.364 99 58 2 12 107 117 213 4.28e-16 72.8

Find 34 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGAGAAATTCACATTTA+AGG 0.174751 7.3:-48898691 MS.gene97477:CDS
TGTTATGAAGTTGATATTCT+TGG 0.275463 7.3:-48898841 None:intergenic
GGACAATATGTCTTTCTTCT+TGG 0.280895 7.3:-48898438 MS.gene97477:CDS
AGTATTGCCCTGCTTATTGT+TGG 0.324545 7.3:-48898724 MS.gene97477:CDS
AGAAACATTGACAATTTAAC+AGG 0.337581 7.3:-48898459 MS.gene97477:CDS
GACAATATGTCTTTCTTCTT+GGG 0.345288 7.3:-48898437 MS.gene97477:intron
GAGCACTATACATTCTCAAC+TGG 0.354642 7.3:-48897699 MS.gene97477:CDS
TGAATTTCTCATTGATAAGT+AGG 0.405613 7.3:+48898700 None:intergenic
CAGTACCAGTTGAGGAAGTA+TGG 0.407791 7.3:+48898494 None:intergenic
TGGCAACAGCTTCCAAATAC+AGG 0.408291 7.3:+48898514 None:intergenic
GTTATGAAGTTGATATTCTT+GGG 0.424245 7.3:-48898840 MS.gene97477:CDS
TGTTGCCATACTTCCTCAAC+TGG 0.450840 7.3:-48898499 MS.gene97477:CDS
CCGAGCCCCACTTTGTCCAT+TGG 0.453725 7.3:-48897660 MS.gene97477:CDS
ATGCTGGCAAATGAAGCTTC+TGG 0.490077 7.3:+48897335 None:intergenic
AAGTAGGGCCAACAATAAGC+AGG 0.512666 7.3:+48898716 None:intergenic
TAGATCATATGACAAAGATC+AGG 0.514146 7.3:-48898769 MS.gene97477:CDS
CCAATGGACAAAGTGGGGCT+CGG 0.529481 7.3:+48897660 None:intergenic
TACTTATCCAATGGACAAAG+TGG 0.529817 7.3:+48897653 None:intergenic
TATGGAGAATTACACTATGC+TGG 0.546192 7.3:+48897319 None:intergenic
CTTTGTAGCAGTACCAGTTG+AGG 0.551696 7.3:+48898486 None:intergenic
TTTCTATTGTATGGTACATG+AGG 0.554169 7.3:-48898809 MS.gene97477:CDS
TCTTCTACTACTATTTCCAA+AGG 0.567268 7.3:-48897459 MS.gene97477:intron
TGAGAAATTCACATTTAAGG+AGG 0.576216 7.3:-48898688 MS.gene97477:intron
GAATTTCTCATTGATAAGTA+GGG 0.579233 7.3:+48898701 None:intergenic
ACTTATCCAATGGACAAAGT+GGG 0.585109 7.3:+48897654 None:intergenic
TACTAGAAAATAGTGACGGA+AGG 0.599670 7.3:+48898743 None:intergenic
ACAGAATTATACTTATCCAA+TGG 0.602023 7.3:+48897644 None:intergenic
GGCAACAGCTTCCAAATACA+GGG 0.635959 7.3:+48898515 None:intergenic
CTTATCCAATGGACAAAGTG+GGG 0.636328 7.3:+48897655 None:intergenic
TATACATGGAATAGGTGATG+TGG 0.652075 7.3:-48898545 MS.gene97477:intron
AGCACTATACATTCTCAACT+GGG 0.652774 7.3:-48897698 MS.gene97477:CDS
GCAATACTAGAAAATAGTGA+CGG 0.652982 7.3:+48898739 None:intergenic
ATGATCTACGAACAATCTTG+TGG 0.654874 7.3:+48898784 None:intergenic
AGTAGGGCCAACAATAAGCA+GGG 0.670399 7.3:+48898717 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATTATTAACATAAATAAAA+TGG + chr7.3:48898759-48898778 None:intergenic 10.0%
!! ATGCAAATATATAATGTTAA+AGG + chr7.3:48897770-48897789 None:intergenic 15.0%
!! CTAGATAAATTATAGCTAAT+GGG - chr7.3:48898091-48898110 MS.gene97477:intron 20.0%
!! GCTATAATAAAAATAAGCAA+TGG - chr7.3:48898337-48898356 MS.gene97477:intron 20.0%
!! TAAAAACATTCAAACATGTA+TGG + chr7.3:48897553-48897572 None:intergenic 20.0%
!! TAGATAAATTATAGCTAATG+GGG - chr7.3:48898092-48898111 MS.gene97477:intron 20.0%
!! TTATATATTTGCATGATTTC+TGG - chr7.3:48897776-48897795 MS.gene97477:intron 20.0%
!!! AGTTGAAAGTTTTTATTTGT+TGG - chr7.3:48898225-48898244 MS.gene97477:intron 20.0%
!!! TCTTGTTTAATTGTTATCAT+TGG - chr7.3:48898550-48898569 MS.gene97477:intron 20.0%
! AAAGCTAAAACAAGTTCAAA+TGG + chr7.3:48898385-48898404 None:intergenic 25.0%
! AAGCTAAAACAAGTTCAAAT+GGG + chr7.3:48898384-48898403 None:intergenic 25.0%
! AATTAGCAGCTATTATATTC+TGG - chr7.3:48897823-48897842 MS.gene97477:intron 25.0%
! ACAGAATTATACTTATCCAA+TGG + chr7.3:48898529-48898548 None:intergenic 25.0%
! ACTGTAATGTTTAACAATGA+TGG + chr7.3:48898604-48898623 None:intergenic 25.0%
! AGAAACATTGACAATTTAAC+AGG - chr7.3:48897711-48897730 MS.gene97477:intron 25.0%
! ATCATTGTTAAACATTACAG+TGG - chr7.3:48898603-48898622 MS.gene97477:intron 25.0%
! CAATGAGAAATTCACATTTA+AGG - chr7.3:48897479-48897498 MS.gene97477:CDS 25.0%
! CCATTAGCTATAATTTATCT+AGG + chr7.3:48898093-48898112 None:intergenic 25.0%
! CCTAGATAAATTATAGCTAA+TGG - chr7.3:48898090-48898109 MS.gene97477:intron 25.0%
! GAATTTCTCATTGATAAGTA+GGG + chr7.3:48897472-48897491 None:intergenic 25.0%
! TACTATTTCCAAAGGTAAAT+TGG - chr7.3:48898719-48898738 MS.gene97477:CDS 25.0%
! TATAATTTATCTAGGTGAGT+TGG + chr7.3:48898085-48898104 None:intergenic 25.0%
! TGAATTTCTCATTGATAAGT+AGG + chr7.3:48897473-48897492 None:intergenic 25.0%
! TTTCATGTAACATGTTGTAT+AGG - chr7.3:48898043-48898062 MS.gene97477:intron 25.0%
!!! TTGAACTTGTTTTAGCTTTT+AGG - chr7.3:48898386-48898405 MS.gene97477:intron 25.0%
AAACTGCTTATACATGGAAT+AGG - chr7.3:48897617-48897636 MS.gene97477:intron 30.0%
AACCTTAATAAGCAGAACTT+TGG + chr7.3:48898283-48898302 None:intergenic 30.0%
ATGGTAACTTTGTATATCAG+AGG - chr7.3:48898356-48898375 MS.gene97477:intron 30.0%
CAACTCAAGATCATAATAGA+AGG + chr7.3:48898210-48898229 None:intergenic 30.0%
GACAATATGTCTTTCTTCTT+GGG - chr7.3:48897733-48897752 MS.gene97477:intron 30.0%
GCAATACTAGAAAATAGTGA+CGG + chr7.3:48897434-48897453 None:intergenic 30.0%
TAGATCATATGACAAAGATC+AGG - chr7.3:48897401-48897420 MS.gene97477:intron 30.0%
TCTTCTACTACTATTTCCAA+AGG - chr7.3:48898711-48898730 MS.gene97477:CDS 30.0%
TGAAAATATTGCCTTGTAGT+TGG - chr7.3:48898804-48898823 MS.gene97477:CDS 30.0%
TGAGAAATTCACATTTAAGG+AGG - chr7.3:48897482-48897501 MS.gene97477:CDS 30.0%
TTTCTATTGTATGGTACATG+AGG - chr7.3:48897361-48897380 MS.gene97477:intron 30.0%
! ATTCCATGTATAAGCAGTTT+TGG + chr7.3:48897617-48897636 None:intergenic 30.0%
! GAAGCTCATTTTCTATTGTA+TGG - chr7.3:48897352-48897371 MS.gene97477:intron 30.0%
! TATATTTGCATGATTTCTGG+TGG - chr7.3:48897779-48897798 MS.gene97477:intron 30.0%
! TTCCATGTATAAGCAGTTTT+GGG + chr7.3:48897616-48897635 None:intergenic 30.0%
! TTTATTTGTTGGAACGAAAC+TGG - chr7.3:48898236-48898255 MS.gene97477:intron 30.0%
ACTTATCCAATGGACAAAGT+GGG + chr7.3:48898519-48898538 None:intergenic 35.0%
AGCACTATACATTCTCAACT+GGG - chr7.3:48898472-48898491 MS.gene97477:CDS 35.0%
AGCCAAAGTTCTGCTTATTA+AGG - chr7.3:48898278-48898297 MS.gene97477:intron 35.0%
ATAAAATGGACCTGACAAGT+AGG + chr7.3:48898745-48898764 None:intergenic 35.0%
ATGATCTACGAACAATCTTG+TGG + chr7.3:48897389-48897408 None:intergenic 35.0%
GGACAATATGTCTTTCTTCT+TGG - chr7.3:48897732-48897751 MS.gene97477:intron 35.0%
GGCTTCAAGATCATAATAGA+AGG + chr7.3:48898262-48898281 None:intergenic 35.0%
TACTTATCCAATGGACAAAG+TGG + chr7.3:48898520-48898539 None:intergenic 35.0%
TATACATGGAATAGGTGATG+TGG - chr7.3:48897625-48897644 MS.gene97477:intron 35.0%
TTGAAACTGATCAATGAGAC+TGG - chr7.3:48898184-48898203 MS.gene97477:intron 35.0%
TTGGTTTATGCTCTTTAGAC+TGG - chr7.3:48898569-48898588 MS.gene97477:intron 35.0%
TTTCAATGAAGCCAACAAAC+AGG + chr7.3:48898170-48898189 None:intergenic 35.0%
! CATTACTTCTTTTGCAGCAT+GGG - chr7.3:48898652-48898671 MS.gene97477:intron 35.0%
! TACTAGAAAATAGTGACGGA+AGG + chr7.3:48897430-48897449 None:intergenic 35.0%
AATGGAAGGAAGTCAGAACA+AGG + chr7.3:48898636-48898655 None:intergenic 40.0%
AGACCAACCTTCCAGTTATT+TGG + chr7.3:48897874-48897893 None:intergenic 40.0%
AGTATTGCCCTGCTTATTGT+TGG - chr7.3:48897446-48897465 MS.gene97477:intron 40.0%
CAAGTAGGCCAATTTACCTT+TGG + chr7.3:48898730-48898749 None:intergenic 40.0%
CCATGCTGCAAAAGAAGTAA+TGG + chr7.3:48898654-48898673 None:intergenic 40.0%
CTGATCAACAGCCAAATAAC+TGG - chr7.3:48897860-48897879 MS.gene97477:intron 40.0%
CTTATCCAATGGACAAAGTG+GGG + chr7.3:48898518-48898537 None:intergenic 40.0%
CTTTCTTCTTGGGTAAGCTA+TGG - chr7.3:48897743-48897762 MS.gene97477:intron 40.0%
CTTTGTCTGTGAAATGCAGT+GGG - chr7.3:48898444-48898463 MS.gene97477:CDS 40.0%
GAGCACTATACATTCTCAAC+TGG - chr7.3:48898471-48898490 MS.gene97477:CDS 40.0%
GCTCAAAACTAAGACGAACA+AGG + chr7.3:48898781-48898800 None:intergenic 40.0%
GTCCCAAAACTGCTTATACA+TGG - chr7.3:48897611-48897630 MS.gene97477:intron 40.0%
TAGCTAATGGGGACAACAAA+AGG - chr7.3:48898103-48898122 MS.gene97477:intron 40.0%
TCAACAGCCAAATAACTGGA+AGG - chr7.3:48897864-48897883 MS.gene97477:intron 40.0%
TGGTTGTTCTTCCAACTACA+AGG + chr7.3:48898818-48898837 None:intergenic 40.0%
! CCATTACTTCTTTTGCAGCA+TGG - chr7.3:48898651-48898670 MS.gene97477:intron 40.0%
! GCTGCAAAAGAAGTAATGGA+AGG + chr7.3:48898650-48898669 None:intergenic 40.0%
! GTGAGTTGGATTGACAACTT+TGG + chr7.3:48898071-48898090 None:intergenic 40.0%
! GTGGACATTTTCCCTGTATT+TGG - chr7.3:48897644-48897663 MS.gene97477:intron 40.0%
AAGTAGGGCCAACAATAAGC+AGG + chr7.3:48897457-48897476 None:intergenic 45.0%
AGTAGGGCCAACAATAAGCA+GGG + chr7.3:48897456-48897475 None:intergenic 45.0%
ATGCTGGCAAATGAAGCTTC+TGG + chr7.3:48898838-48898857 None:intergenic 45.0%
CAGCCAAATAACTGGAAGGT+TGG - chr7.3:48897868-48897887 MS.gene97477:intron 45.0%
CAGTACCAGTTGAGGAAGTA+TGG + chr7.3:48897679-48897698 None:intergenic 45.0%
CTTTGTAGCAGTACCAGTTG+AGG + chr7.3:48897687-48897706 None:intergenic 45.0%
GCATAGAACGAGCTTAGTAG+TGG - chr7.3:48897912-48897931 MS.gene97477:intron 45.0%
GCTTTGTCTGTGAAATGCAG+TGG - chr7.3:48898443-48898462 MS.gene97477:CDS 45.0%
GGCAACAGCTTCCAAATACA+GGG + chr7.3:48897658-48897677 None:intergenic 45.0%
TGGCAACAGCTTCCAAATAC+AGG + chr7.3:48897659-48897678 None:intergenic 45.0%
TGTTGCCATACTTCCTCAAC+TGG - chr7.3:48897671-48897690 MS.gene97477:CDS 45.0%
! CTTTTGCAGCATGGGTTTCT+GGG - chr7.3:48898660-48898679 MS.gene97477:intron 45.0%
! GGTAAATTGGCCTACTTGTC+AGG - chr7.3:48898732-48898751 MS.gene97477:CDS 45.0%
! TCTTTTGCAGCATGGGTTTC+TGG - chr7.3:48898659-48898678 MS.gene97477:intron 45.0%
!! TTAGTTTGCAGTAGTGCTCG+TGG - chr7.3:48898139-48898158 MS.gene97477:intron 45.0%
TCGTGGACAAGCCTGTTTGT+TGG - chr7.3:48898156-48898175 MS.gene97477:intron 50.0%
CCAATGGACAAAGTGGGGCT+CGG + chr7.3:48898513-48898532 None:intergenic 55.0%
CCGAGCCCCACTTTGTCCAT+TGG - chr7.3:48898510-48898529 MS.gene97477:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 48897333 48898859 48897333 ID=MS.gene97477
chr7.3 mRNA 48897333 48898859 48897333 ID=MS.gene97477.t1;Parent=MS.gene97477
chr7.3 exon 48898689 48898859 48898689 ID=MS.gene97477.t1.exon1;Parent=MS.gene97477.t1
chr7.3 CDS 48898689 48898859 48898689 ID=cds.MS.gene97477.t1;Parent=MS.gene97477.t1
chr7.3 exon 48898438 48898553 48898438 ID=MS.gene97477.t1.exon2;Parent=MS.gene97477.t1
chr7.3 CDS 48898438 48898553 48898438 ID=cds.MS.gene97477.t1;Parent=MS.gene97477.t1
chr7.3 exon 48897656 48897729 48897656 ID=MS.gene97477.t1.exon3;Parent=MS.gene97477.t1
chr7.3 CDS 48897656 48897729 48897656 ID=cds.MS.gene97477.t1;Parent=MS.gene97477.t1
chr7.3 exon 48897460 48897523 48897460 ID=MS.gene97477.t1.exon4;Parent=MS.gene97477.t1
chr7.3 CDS 48897460 48897523 48897460 ID=cds.MS.gene97477.t1;Parent=MS.gene97477.t1
chr7.3 exon 48897333 48897369 48897333 ID=MS.gene97477.t1.exon5;Parent=MS.gene97477.t1
chr7.3 CDS 48897333 48897369 48897333 ID=cds.MS.gene97477.t1;Parent=MS.gene97477.t1
Gene Sequence

>MS.gene97477

ATGAAGTTGATATTCTTGGGAAGCTCATTTTCTATTGTATGGTACATGAGGTACCACAAGATTGTTCGTAGATCATATGACAAAGATCAGGATACCTTCCGTCACTATTTTCTAGTATTGCCCTGCTTATTGTTGGCCCTACTTATCAATGAGAAATTCACATTTAAGGAGGTTAGAATGCATTCACTGTTGTCTATCTTCTGTAACTGTTGTGCTGCCATACATGTTTGAATGTTTTTAAGAGATGATGACTTTGCATTAATTAGCTTTCGTTTGTTGTCCCAAAACTGCTTATACATGGAATAGGTGATGTGGACATTTTCCCTGTATTTGGAAGCTGTTGCCATACTTCCTCAACTGGTACTGCTACAAAGAACTAGAAACATTGACAATTTAACAGGACAATATGTCTTTCTTCTTGGGTAAGCTATGGTCCTTTAACATTATATATTTGCATGATTTCTGGTGGCTGTTATTGCATTAGTATTGAAATTAGCAGCTATTATATTCTGGAAGTATTTCAGTTACTGATCAACAGCCAAATAACTGGAAGGTTGGTCTATGTTGATATCTATTTCTGCATAGAACGAGCTTAGTAGTGGTTTCATGCATGATAATGATGATGTAAATCGTAGTTGCAATTTGATACAGTTCTGAGTCATTGAACTAATGCTCTTTAAATAGTGATTCACATGAAGAAAATCATAGCATTTCATGTAACATGTTGTATAGGTTCCAAAGTTGTCAATCCAACTCACCTAGATAAATTATAGCTAATGGGGACAACAAAAGGAATAGAAAAATTATTAGTTTGCAGTAGTGCTCGTGGACAAGCCTGTTTGTTGGCTTCATTGAAACTGATCAATGAGACTGGCCTTCTATTATGATCTTGAGTTGAAAGTTTTTATTTGTTGGAACGAAACTGGCCTTCTATTATGATCTTGAAGCCAAAGTTCTGCTTATTAAGGTTTTCAAGTTTATGCAACTAGCTCTACATATAGTGTGCTATAATAAAAATAAGCAATGGTAACTTTGTATATCAGAGGAACCCATTTGAACTTGTTTTAGCTTTTAGGCTTTAAGATCTTAGAATTCTGATTCAATAATATTGCTTTGTCTGTGAAATGCAGTGGGTACAGAGCACTATACATTCTCAACTGGGTCTATCGCTACTTTACCGAGCCCCACTTTGTCCATTGGATAAGTATAATTCTGTCTCTTGTTTAATTGTTATCATTGGTTTATGCTCTTTAGACTGGAAAACTTTTCCATCATTGTTAAACATTACAGTGGCTATAAACCTTGTTCTGACTTCCTTCCATTACTTCTTTTGCAGCATGGGTTTCTGGGCTTGTTCAAACACTGCTATATGCTGACTTCTTCTACTACTATTTCCAAAGGTAAATTGGCCTACTTGTCAGGTCCATTTTATTTATGTTAATAATCCTTGTTCGTCTTAGTTTTGAGCATTTGAAAATATTGCCTTGTAGTTGGAAGAACAACCAGAAGCTTCATTTGCCAGCATAG

Protein sequence

>MS.gene97477.t1

MKLIFLGSSFSIVWYMRYHKIVRRSYDKDQDTFRHYFLVLPCLLLALLINEKFTFKEVMWTFSLYLEAVAILPQLVLLQRTRNIDNLTGQYVFLLGGYRALYILNWVYRYFTEPHFVHWITWVSGLVQTLLYADFFYYYFQSWKNNQKLHLPA