Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98318.t1 | XP_013460895.1 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 3.30E-117 | 431 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98318.t1 | A0A072UYT0 | 96.8 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 2.4e-117 | 431.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene98318 | 0.806293 | 8.83E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene98318 | 0.800546 | 1.37E-48 | -1.69E-46 |
| MS.gene049758 | MS.gene98318 | 0.824448 | 8.03E-54 | -1.69E-46 |
| MS.gene050020 | MS.gene98318 | 0.813743 | 2.19E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene98318 | 0.825727 | 4.00E-54 | -1.69E-46 |
| MS.gene050584 | MS.gene98318 | 0.834576 | 2.77E-56 | -1.69E-46 |
| MS.gene050585 | MS.gene98318 | 0.806192 | 9.27E-50 | -1.69E-46 |
| MS.gene050586 | MS.gene98318 | 0.829129 | 6.12E-55 | -1.69E-46 |
| MS.gene050762 | MS.gene98318 | 0.800716 | 1.26E-48 | -1.69E-46 |
| MS.gene050763 | MS.gene98318 | 0.802814 | 4.69E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene98318 | 0.851193 | 1.04E-60 | -1.69E-46 |
| MS.gene051662 | MS.gene98318 | 0.801275 | 9.72E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene98318 | 0.809358 | 1.97E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene98318 | 0.898819 | 3.65E-77 | -1.69E-46 |
| MS.gene052142 | MS.gene98318 | 0.80223 | 6.19E-49 | -1.69E-46 |
| MS.gene052178 | MS.gene98318 | 0.857409 | 1.67E-62 | -1.69E-46 |
| MS.gene052337 | MS.gene98318 | 0.800628 | 1.32E-48 | -1.69E-46 |
| MS.gene052967 | MS.gene98318 | 0.836286 | 1.02E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene98318 | 0.879517 | 1.17E-69 | -1.69E-46 |
| MS.gene053204 | MS.gene98318 | 0.811844 | 5.71E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene98318 | 0.824924 | 6.20E-54 | -1.69E-46 |
| MS.gene054011 | MS.gene98318 | 0.819327 | 1.23E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene98318 | 0.813579 | 2.38E-51 | -1.69E-46 |
| MS.gene054013 | MS.gene98318 | 0.809158 | 2.17E-50 | -1.69E-46 |
| MS.gene054184 | MS.gene98318 | 0.833449 | 5.30E-56 | -1.69E-46 |
| MS.gene054185 | MS.gene98318 | 0.839693 | 1.36E-57 | -1.69E-46 |
| MS.gene054214 | MS.gene98318 | 0.864703 | 1.00E-64 | -1.69E-46 |
| MS.gene05456 | MS.gene98318 | 0.847671 | 9.98E-60 | -1.69E-46 |
| MS.gene054596 | MS.gene98318 | 0.831488 | 1.62E-55 | -1.69E-46 |
| MS.gene055133 | MS.gene98318 | 0.818651 | 1.75E-52 | -1.69E-46 |
| MS.gene055207 | MS.gene98318 | 0.830236 | 3.29E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene98318 | 0.805479 | 1.31E-49 | -1.69E-46 |
| MS.gene055428 | MS.gene98318 | 0.80281 | 4.70E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene98318 | 0.809838 | 1.55E-50 | -1.69E-46 |
| MS.gene056069 | MS.gene98318 | -0.823367 | 1.44E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene98318 | 0.818058 | 2.39E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene98318 | 0.835808 | 1.35E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene98318 | 0.881632 | 2.04E-70 | -1.69E-46 |
| MS.gene059124 | MS.gene98318 | 0.81816 | 2.27E-52 | -1.69E-46 |
| MS.gene059228 | MS.gene98318 | 0.839003 | 2.05E-57 | -1.69E-46 |
| MS.gene06037 | MS.gene98318 | 0.812176 | 4.84E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene98318 | 0.842471 | 2.53E-58 | -1.69E-46 |
| MS.gene061049 | MS.gene98318 | 0.814239 | 1.70E-51 | -1.69E-46 |
| MS.gene061112 | MS.gene98318 | 0.809367 | 1.96E-50 | -1.69E-46 |
| MS.gene061265 | MS.gene98318 | 0.8093 | 2.03E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98318.t1 | MTR_3g074450 | 96.818 | 220 | 7 | 0 | 1 | 220 | 1 | 220 | 1.07e-159 | 440 |
| MS.gene98318.t1 | MTR_5g063720 | 53.540 | 226 | 82 | 10 | 1 | 217 | 1 | 212 | 3.83e-58 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98318.t1 | AT1G78170 | 34.804 | 204 | 111 | 8 | 18 | 211 | 21 | 212 | 2.00e-19 | 83.6 |
| MS.gene98318.t1 | AT1G22250 | 40.870 | 115 | 55 | 4 | 101 | 208 | 80 | 188 | 4.83e-16 | 73.9 |
| MS.gene98318.t1 | AT4G08910 | 40.000 | 120 | 45 | 3 | 102 | 203 | 86 | 196 | 1.16e-15 | 73.2 |
Find 43 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATGTTTCCTTCAAATAAT+TGG | 0.175659 | 3.1:+61039212 | MS.gene98318:CDS |
| TTGAAGAATTCTTCAGTAAA+TGG | 0.216211 | 3.1:-61039130 | None:intergenic |
| GTGTCAATACCTTGGTATTC+AGG | 0.222403 | 3.1:+61039234 | MS.gene98318:CDS |
| TCTTCTTTAACTTCAGTTCT+TGG | 0.222864 | 3.1:-61039073 | None:intergenic |
| CATGGACAGTGCTTTGAATA+AGG | 0.336246 | 3.1:-61039047 | None:intergenic |
| CAGCTTCTTCTGCTCTAGTT+AGG | 0.340324 | 3.1:+61039775 | MS.gene98318:CDS |
| AGCAAGTTTAAGAAAGATTC+GGG | 0.373167 | 3.1:+61039569 | MS.gene98318:CDS |
| AAGCAAGTTTAAGAAAGATT+CGG | 0.375998 | 3.1:+61039568 | MS.gene98318:CDS |
| CATCTCTTTGTAATCTCCTT+CGG | 0.379098 | 3.1:-61039627 | None:intergenic |
| ATCATGTTCTGTCAAACTCT+TGG | 0.401354 | 3.1:-61039528 | None:intergenic |
| AAAGATTCGGGTGGTTTAAA+CGG | 0.409779 | 3.1:+61039581 | MS.gene98318:CDS |
| GTTTCTATCGTTGTCCGATC+GGG | 0.412127 | 3.1:-61039380 | None:intergenic |
| ATTTACTGAAGAATTCTTCA+AGG | 0.415230 | 3.1:+61039132 | MS.gene98318:CDS |
| ATCAAACAAATTTAGAGAAA+AGG | 0.427149 | 3.1:+61039155 | MS.gene98318:CDS |
| TGAAGGAAACATGGTCTCTA+GGG | 0.443563 | 3.1:-61039203 | None:intergenic |
| GGTTTCTATCGTTGTCCGAT+CGG | 0.443816 | 3.1:-61039381 | None:intergenic |
| TATTGACACCAATTATTTGA+AGG | 0.449231 | 3.1:-61039220 | None:intergenic |
| TTGTCGCAGGCTTCGTTTCG+AGG | 0.449883 | 3.1:-61039402 | None:intergenic |
| TCATGTTCTGTCAAACTCTT+GGG | 0.450709 | 3.1:-61039527 | None:intergenic |
| TTGAAGGAAACATGGTCTCT+AGG | 0.459950 | 3.1:-61039204 | None:intergenic |
| AGTGATCAGAAGAAGAATTC+TGG | 0.461088 | 3.1:+61039497 | MS.gene98318:CDS |
| AACTTCAGTTCTTGGAGTCA+TGG | 0.494815 | 3.1:-61039065 | None:intergenic |
| ATCAAAGAAAAGAAAGTTTG+AGG | 0.498556 | 3.1:+61039105 | MS.gene98318:CDS |
| GAGTGCATGAATTTGCAGTT+AGG | 0.515352 | 3.1:-61039713 | None:intergenic |
| AATAATTGGTGTCAATACCT+TGG | 0.524933 | 3.1:+61039226 | MS.gene98318:CDS |
| GTTTAAACGGTTCTCAGTCG+TGG | 0.546535 | 3.1:+61039594 | MS.gene98318:CDS |
| TAGGGGAGGCTTTGTATCAA+AGG | 0.546793 | 3.1:-61039185 | None:intergenic |
| AATTTGCAGTTAGGACAAGC+AGG | 0.557243 | 3.1:-61039704 | None:intergenic |
| CCAATTATTTGAAGGAAACA+TGG | 0.558834 | 3.1:-61039212 | None:intergenic |
| CGAAGGAGATTACAAAGAGA+TGG | 0.571998 | 3.1:+61039628 | MS.gene98318:CDS |
| CACATGTCAAGTTTAGCTCA+AGG | 0.577317 | 3.1:-61039451 | None:intergenic |
| TGCTTTGAATAAGGATACCA+TGG | 0.582273 | 3.1:-61039038 | None:intergenic |
| AAGCTCATGTGATCAGAAGA+AGG | 0.583375 | 3.1:-61039425 | None:intergenic |
| GGGAGGCTTTGTATCAAAGG+TGG | 0.591243 | 3.1:-61039182 | None:intergenic |
| GATCAGAAGAAGGTTGTCGC+AGG | 0.608082 | 3.1:-61039415 | None:intergenic |
| TCGTGGTTGTCGACAACCGA+AGG | 0.619983 | 3.1:+61039611 | MS.gene98318:CDS |
| TGTGTAACACAAGACCCGAT+CGG | 0.652579 | 3.1:+61039366 | MS.gene98318:CDS |
| ACTTGACATGTGAATCACAA+AGG | 0.656443 | 3.1:+61039462 | MS.gene98318:CDS |
| GGAAACATGGTCTCTAGGGG+AGG | 0.657415 | 3.1:-61039199 | None:intergenic |
| TTGCATGCAGTGTCACATGT+TGG | 0.659365 | 3.1:+61039661 | MS.gene98318:CDS |
| GAAGGAAACATGGTCTCTAG+GGG | 0.665977 | 3.1:-61039202 | None:intergenic |
| AAGTTTAAGAAAGATTCGGG+TGG | 0.705138 | 3.1:+61039572 | MS.gene98318:CDS |
| CAACATATACCTGAATACCA+AGG | 0.712025 | 3.1:-61039243 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAAACAAATTTAGAGAAA+AGG | + | chr3.1:61039155-61039174 | MS.gene98318:CDS | 20.0% |
| !! | ATCATAAGATATGATATCAT+TGG | - | chr3.1:61039304-61039323 | None:intergenic | 20.0% |
| ! | AAGCAAGTTTAAGAAAGATT+CGG | + | chr3.1:61039568-61039587 | MS.gene98318:CDS | 25.0% |
| ! | ATCAAAGAAAAGAAAGTTTG+AGG | + | chr3.1:61039105-61039124 | MS.gene98318:CDS | 25.0% |
| ! | ATTTACTGAAGAATTCTTCA+AGG | + | chr3.1:61039132-61039151 | MS.gene98318:CDS | 25.0% |
| ! | TATTGACACCAATTATTTGA+AGG | - | chr3.1:61039223-61039242 | None:intergenic | 25.0% |
| ! | TTGAAGAATTCTTCAGTAAA+TGG | - | chr3.1:61039133-61039152 | None:intergenic | 25.0% |
| !!! | AATTTTTTTTATGTGCAGTC+AGG | + | chr3.1:61039332-61039351 | MS.gene98318:intron | 25.0% |
| !!! | ATTTTTTTTATGTGCAGTCA+GGG | + | chr3.1:61039333-61039352 | MS.gene98318:intron | 25.0% |
| AGCAAGTTTAAGAAAGATTC+GGG | + | chr3.1:61039569-61039588 | MS.gene98318:CDS | 30.0% | |
| CCAATTATTTGAAGGAAACA+TGG | - | chr3.1:61039215-61039234 | None:intergenic | 30.0% | |
| CCATGTTTCCTTCAAATAAT+TGG | + | chr3.1:61039212-61039231 | MS.gene98318:CDS | 30.0% | |
| ! | TCTTCTTTAACTTCAGTTCT+TGG | - | chr3.1:61039076-61039095 | None:intergenic | 30.0% |
| ! | TGCTCTTCAAGAATTTTGAA+GGG | - | chr3.1:61039751-61039770 | None:intergenic | 30.0% |
| !! | AATAATTGGTGTCAATACCT+TGG | + | chr3.1:61039226-61039245 | MS.gene98318:CDS | 30.0% |
| AAAGATTCGGGTGGTTTAAA+CGG | + | chr3.1:61039581-61039600 | MS.gene98318:CDS | 35.0% | |
| AAGTTTAAGAAAGATTCGGG+TGG | + | chr3.1:61039572-61039591 | MS.gene98318:CDS | 35.0% | |
| ACTTGACATGTGAATCACAA+AGG | + | chr3.1:61039462-61039481 | MS.gene98318:CDS | 35.0% | |
| AGTGATCAGAAGAAGAATTC+TGG | + | chr3.1:61039497-61039516 | MS.gene98318:CDS | 35.0% | |
| ATCATGTTCTGTCAAACTCT+TGG | - | chr3.1:61039531-61039550 | None:intergenic | 35.0% | |
| CAACATATACCTGAATACCA+AGG | - | chr3.1:61039246-61039265 | None:intergenic | 35.0% | |
| CATCTCTTTGTAATCTCCTT+CGG | - | chr3.1:61039630-61039649 | None:intergenic | 35.0% | |
| TCATGTTCTGTCAAACTCTT+GGG | - | chr3.1:61039530-61039549 | None:intergenic | 35.0% | |
| ! | CTGCTCTTCAAGAATTTTGA+AGG | - | chr3.1:61039752-61039771 | None:intergenic | 35.0% |
| !! | TCAAGAATTTTGAAGGGTTC+TGG | - | chr3.1:61039745-61039764 | None:intergenic | 35.0% |
| AACTTCAGTTCTTGGAGTCA+TGG | - | chr3.1:61039068-61039087 | None:intergenic | 40.0% | |
| AAGCTCATGTGATCAGAAGA+AGG | - | chr3.1:61039428-61039447 | None:intergenic | 40.0% | |
| AATTTGCAGTTAGGACAAGC+AGG | - | chr3.1:61039707-61039726 | None:intergenic | 40.0% | |
| CGAAGGAGATTACAAAGAGA+TGG | + | chr3.1:61039628-61039647 | MS.gene98318:CDS | 40.0% | |
| GAGTGCATGAATTTGCAGTT+AGG | - | chr3.1:61039716-61039735 | None:intergenic | 40.0% | |
| GTGTCAATACCTTGGTATTC+AGG | + | chr3.1:61039234-61039253 | MS.gene98318:CDS | 40.0% | |
| TGAAGGAAACATGGTCTCTA+GGG | - | chr3.1:61039206-61039225 | None:intergenic | 40.0% | |
| TTGAAGGAAACATGGTCTCT+AGG | - | chr3.1:61039207-61039226 | None:intergenic | 40.0% | |
| ! | CACATGTCAAGTTTAGCTCA+AGG | - | chr3.1:61039454-61039473 | None:intergenic | 40.0% |
| ! | CATGGACAGTGCTTTGAATA+AGG | - | chr3.1:61039050-61039069 | None:intergenic | 40.0% |
| !! | AATTTTGAAGGGTTCTGGTC+TGG | - | chr3.1:61039740-61039759 | None:intergenic | 40.0% |
| GAAGGAAACATGGTCTCTAG+GGG | - | chr3.1:61039205-61039224 | None:intergenic | 45.0% | |
| GGTTTCTATCGTTGTCCGAT+CGG | - | chr3.1:61039384-61039403 | None:intergenic | 45.0% | |
| GTTTAAACGGTTCTCAGTCG+TGG | + | chr3.1:61039594-61039613 | MS.gene98318:CDS | 45.0% | |
| GTTTCTATCGTTGTCCGATC+GGG | - | chr3.1:61039383-61039402 | None:intergenic | 45.0% | |
| TGTGTAACACAAGACCCGAT+CGG | + | chr3.1:61039366-61039385 | MS.gene98318:CDS | 45.0% | |
| TTGCATGCAGTGTCACATGT+TGG | + | chr3.1:61039661-61039680 | MS.gene98318:CDS | 45.0% | |
| ! | TAGGGGAGGCTTTGTATCAA+AGG | - | chr3.1:61039188-61039207 | None:intergenic | 45.0% |
| GATCAGAAGAAGGTTGTCGC+AGG | - | chr3.1:61039418-61039437 | None:intergenic | 50.0% | |
| ! | GGGAGGCTTTGTATCAAAGG+TGG | - | chr3.1:61039185-61039204 | None:intergenic | 50.0% |
| TCGTGGTTGTCGACAACCGA+AGG | + | chr3.1:61039611-61039630 | MS.gene98318:CDS | 55.0% | |
| TTGTCGCAGGCTTCGTTTCG+AGG | - | chr3.1:61039405-61039424 | None:intergenic | 55.0% | |
| ! | GGAAACATGGTCTCTAGGGG+AGG | - | chr3.1:61039202-61039221 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 61039040 | 61039796 | 61039040 | ID=MS.gene98318 |
| chr3.1 | mRNA | 61039040 | 61039796 | 61039040 | ID=MS.gene98318.t1;Parent=MS.gene98318 |
| chr3.1 | exon | 61039040 | 61039255 | 61039040 | ID=MS.gene98318.t1.exon1;Parent=MS.gene98318.t1 |
| chr3.1 | CDS | 61039040 | 61039255 | 61039040 | ID=cds.MS.gene98318.t1;Parent=MS.gene98318.t1 |
| chr3.1 | exon | 61039350 | 61039796 | 61039350 | ID=MS.gene98318.t1.exon2;Parent=MS.gene98318.t1 |
| chr3.1 | CDS | 61039350 | 61039796 | 61039350 | ID=cds.MS.gene98318.t1;Parent=MS.gene98318.t1 |
| Gene Sequence |
| Protein sequence |
