AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037109.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037109.01.T01 MTR_7g028160 95.848 289 7 2 1 289 1 284 0.0 556
MsG0780037109.01.T01 MTR_4g108370 77.907 86 19 0 29 114 74 159 2.23e-42 151
MsG0780037109.01.T01 MTR_5g039600 58.779 131 36 4 33 146 49 178 4.30e-40 144
MsG0780037109.01.T01 MTR_8g033070 75.610 82 20 0 48 129 76 157 1.35e-38 138
MsG0780037109.01.T01 MTR_2g006150 60.000 120 39 2 12 125 93 209 5.89e-38 139
MsG0780037109.01.T01 MTR_2g006150 60.000 120 39 2 12 125 93 209 6.36e-38 140
MsG0780037109.01.T01 MTR_1g101810 71.765 85 24 0 45 129 57 141 1.02e-36 134
MsG0780037109.01.T01 MTR_1g101810 71.765 85 24 0 45 129 87 171 2.14e-36 133
MsG0780037109.01.T01 MTR_1g114380 75.325 77 19 0 39 115 20 96 2.84e-36 130
MsG0780037109.01.T01 MTR_1g063870 80.882 68 13 0 47 114 44 111 4.61e-35 125
MsG0780037109.01.T01 MTR_1g038650 63.830 94 34 0 48 141 28 121 8.17e-35 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037109.01.T01 AT3G27010 56.126 253 84 9 17 259 39 274 1.21e-71 223
MsG0780037109.01.T01 AT3G27010 56.126 253 84 9 17 259 39 274 1.21e-71 223
MsG0780037109.01.T01 AT1G69690 61.538 117 39 2 1 114 1 114 1.40e-41 146
MsG0780037109.01.T01 AT5G51910 68.132 91 29 0 41 131 46 136 8.88e-39 138
MsG0780037109.01.T01 AT5G51910 68.132 91 29 0 41 131 46 136 8.88e-39 138
MsG0780037109.01.T01 AT2G45680 77.215 79 18 0 48 126 71 149 5.11e-38 137
MsG0780037109.01.T01 AT5G41030 38.938 226 74 11 48 271 64 227 1.29e-37 134
MsG0780037109.01.T01 AT1G58100 75.904 83 14 1 49 125 57 139 6.04e-37 135
MsG0780037109.01.T01 AT3G47620 89.552 67 7 0 48 114 113 179 1.00e-36 137
MsG0780037109.01.T01 AT5G08330 71.605 81 23 0 46 126 25 105 7.27e-35 126
MsG0780037109.01.T01 AT1G35560 57.258 124 41 4 48 165 52 169 1.13e-34 128
MsG0780037109.01.T01 AT1G72010 83.333 66 11 0 49 114 60 125 1.24e-34 129
MsG0780037109.01.T01 AT5G23280 72.368 76 21 0 40 115 27 102 4.38e-34 124
MsG0780037109.01.T01 AT2G37000 81.356 59 11 0 53 111 44 102 4.68e-28 107
MsG0780037109.01.T01 AT3G45150 58.730 63 26 0 49 111 14 76 9.09e-21 87.4
MsG0780037109.01.T01 AT1G58100 51.807 83 10 2 49 125 57 115 1.01e-15 77.0

Find 62 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGCCTGGTTTGGAATT+AGG 0.117190 7:-19704737 MsG0780037109.01.T01:CDS
ATGTTAGTTCTACAAGTTTA+TGG 0.160920 7:-19704955 MsG0780037109.01.T01:CDS
GATGGGCCTGGTGGGCTTGA+TGG 0.187359 7:+19704996 None:intergenic
CAAGGTTCATCTTTGTCTTC+TGG 0.222188 7:-19705055 MsG0780037109.01.T01:CDS
TATTATTATTCTGCTGATTC+TGG 0.237820 7:+19704621 None:intergenic
CATCATCAAGTTGGTGGATA+TGG 0.242402 7:-19704929 MsG0780037109.01.T01:CDS
TATGCCAACAGGAACAAATT+TGG 0.247795 7:-19704807 MsG0780037109.01.T01:CDS
AGTTGAATGATAGGAATAAT+TGG 0.264011 7:-19705027 MsG0780037109.01.T01:CDS
AGATGCCTGGTTTGGAATTA+GGG 0.264298 7:-19704736 MsG0780037109.01.T01:CDS
ATGGGCCTGGTGGGCTTGAT+GGG 0.294523 7:+19704997 None:intergenic
GCTTCAGCTTTAGCTTCTTC+TGG 0.304919 7:-19705094 MsG0780037109.01.T01:CDS
CCTGTTGCAGCTATGATTGA+TGG 0.306906 7:+19705133 None:intergenic
GAATTGTGAGAATCATCTTT+AGG 0.322739 7:+19704596 None:intergenic
TTTCATCAATCATCATCTTC+TGG 0.335762 7:-19704905 MsG0780037109.01.T01:CDS
CTTGATGGGCCTGATGGGCC+TGG 0.347126 7:+19704984 None:intergenic
TTTCAACTCACAAGAGAGTT+AGG 0.358109 7:-19705205 MsG0780037109.01.T01:CDS
AAGGAAGAGGAAGAAGAATA+AGG 0.379567 7:-19705255 MsG0780037109.01.T01:CDS
ATGGGCCTGATGGGCCTGGT+GGG 0.388474 7:+19704988 None:intergenic
CTGGTTTGCAGTTGAATGAT+AGG 0.398808 7:-19705036 MsG0780037109.01.T01:CDS
ATGCCAACAGGAACAAATTT+GGG 0.405289 7:-19704806 MsG0780037109.01.T01:CDS
TCAAGCCCACCAGGCCCATC+AGG 0.414777 7:-19704993 MsG0780037109.01.T01:CDS
ATCATAGCTGCAACAGGAAC+AGG 0.443120 7:-19705127 MsG0780037109.01.T01:CDS
ACTCAGATTTATCAGCAGAT+TGG 0.447988 7:-19704668 MsG0780037109.01.T01:CDS
TCTGGATTTGATATGCCAAC+AGG 0.449552 7:-19704818 MsG0780037109.01.T01:CDS
ACTCCCAAATTTGTTCCTGT+TGG 0.452759 7:+19704803 None:intergenic
GTTGAATGATAGGAATAATT+GGG 0.466051 7:-19705026 MsG0780037109.01.T01:CDS
AAATCAGCAGATGCCTGGTT+TGG 0.470684 7:-19704744 MsG0780037109.01.T01:CDS
AACTAACATGATGACCTTGA+TGG 0.471715 7:+19704969 None:intergenic
ATGACCTTGATGGGCCTGAT+GGG 0.473600 7:+19704979 None:intergenic
CCATCAATCATAGCTGCAAC+AGG 0.479395 7:-19705133 MsG0780037109.01.T01:CDS
GATGGGCCTGATGGGCCTGG+TGG 0.480862 7:+19704987 None:intergenic
TTGTCACAAGATGGACATAT+TGG 0.480930 7:-19704713 MsG0780037109.01.T01:CDS
GAATTAGGGTTGTCACAAGA+TGG 0.488895 7:-19704722 MsG0780037109.01.T01:CDS
ACAATCACAACTTGCAAACA+TGG 0.490040 7:-19705410 MsG0780037109.01.T01:CDS
GACAGACACACAAAAGTTGA+AGG 0.494866 7:-19705274 MsG0780037109.01.T01:CDS
ACTGTTGGTGAAAATAATAG+TGG 0.505174 7:-19704866 MsG0780037109.01.T01:CDS
CACACAAAAGTTGAAGGAAG+AGG 0.510571 7:-19705268 MsG0780037109.01.T01:CDS
TGGCCTCATCATCAAGTTGG+TGG 0.511204 7:-19704935 MsG0780037109.01.T01:CDS
GGTGCAAATCAGCAGATGCC+TGG 0.519917 7:-19704749 MsG0780037109.01.T01:CDS
TCAGACCCATCAAGCCCACC+AGG 0.525810 7:-19705002 MsG0780037109.01.T01:CDS
CAGATGGTGAAACAATTCAA+TGG 0.529264 7:-19705174 MsG0780037109.01.T01:CDS
AGATTGGTCAAAATCAAACT+AGG 0.530078 7:-19704652 MsG0780037109.01.T01:CDS
CAGGCCCATCAGGCCCATCA+AGG 0.530139 7:-19704983 MsG0780037109.01.T01:CDS
ATTCTTGCTGCACATAAAGC+TGG 0.536084 7:+19705229 None:intergenic
AACAGGAACAAATTTGGGAG+TGG 0.545963 7:-19704801 MsG0780037109.01.T01:CDS
GGTTTAGTAGCTACTACTGT+TGG 0.551441 7:-19704881 MsG0780037109.01.T01:CDS
GATGACCTTGATGGGCCTGA+TGG 0.557322 7:+19704978 None:intergenic
ACTAACATGATGACCTTGAT+GGG 0.557914 7:+19704970 None:intergenic
CATCAATCATCATCTTCTGG+TGG 0.564429 7:-19704902 MsG0780037109.01.T01:CDS
GGAACAAATTTGGGAGTGGG+AGG 0.568972 7:-19704797 MsG0780037109.01.T01:CDS
GAAGAAGCTAAAGCTGAAGC+TGG 0.576280 7:+19705097 None:intergenic
GGTAATACTTTGACACCACA+AGG 0.588387 7:-19705073 MsG0780037109.01.T01:CDS
ACAGGAACAAATTTGGGAGT+GGG 0.594534 7:-19704800 MsG0780037109.01.T01:CDS
GATTGGTCAAAATCAAACTA+GGG 0.595101 7:-19704651 MsG0780037109.01.T01:CDS
GAACAAATTTGGGAGTGGGA+GGG 0.598189 7:-19704796 MsG0780037109.01.T01:CDS
TTATGGCCTCATCATCAAGT+TGG 0.605767 7:-19704938 MsG0780037109.01.T01:CDS
GAGTTAGGTCATAAATCAGA+TGG 0.610512 7:-19705190 MsG0780037109.01.T01:CDS
TATCCACCAACTTGATGATG+AGG 0.610804 7:+19704932 None:intergenic
CAATCACAACTTGCAAACAT+GGG 0.617271 7:-19705409 MsG0780037109.01.T01:CDS
GGGAGAGAACAAAGAATCAG+AGG 0.624346 7:-19705389 MsG0780037109.01.T01:CDS
GACAACCCTAATTCCAAACC+AGG 0.628289 7:+19704731 None:intergenic
GAAGACAAAGATGAACCTTG+TGG 0.735014 7:+19705058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTATTTTCAGAAAATT+GGG - Chr7:19705175-19705194 MsG0780037109.01.T01:CDS 15.0%
!!! ATGAGTTTTACATCAATTTT+GGG - Chr7:19705246-19705265 MsG0780037109.01.T01:CDS 20.0%
!!! GGAAATTATTTTCAGAAAAT+TGG - Chr7:19705174-19705193 MsG0780037109.01.T01:CDS 20.0%
! AGTTGAATGATAGGAATAAT+TGG - Chr7:19704992-19705011 MsG0780037109.01.T01:CDS 25.0%
! GTTGAATGATAGGAATAATT+GGG - Chr7:19704993-19705012 MsG0780037109.01.T01:CDS 25.0%
!! GATGAGTTTTACATCAATTT+TGG - Chr7:19705245-19705264 MsG0780037109.01.T01:CDS 25.0%
!! TGAGTTTTACATCAATTTTG+GGG - Chr7:19705247-19705266 MsG0780037109.01.T01:CDS 25.0%
!!! ATGTTAGTTCTACAAGTTTA+TGG - Chr7:19705064-19705083 MsG0780037109.01.T01:CDS 25.0%
!!! TATTATTATTCTGCTGATTC+TGG + Chr7:19705401-19705420 None:intergenic 25.0%
!!! TCTTTGTTTGATGTTCTTTT+TGG + Chr7:19704727-19704746 None:intergenic 25.0%
GAATTGTGAGAATCATCTTT+AGG + Chr7:19705426-19705445 None:intergenic 30.0%
TTTCATCAATCATCATCTTC+TGG - Chr7:19705114-19705133 MsG0780037109.01.T01:CDS 30.0%
! ACTGTTGGTGAAAATAATAG+TGG - Chr7:19705153-19705172 MsG0780037109.01.T01:CDS 30.0%
! AGATTGGTCAAAATCAAACT+AGG - Chr7:19705367-19705386 MsG0780037109.01.T01:CDS 30.0%
! AGTTTTACATCAATTTTGGG+GGG - Chr7:19705249-19705268 MsG0780037109.01.T01:CDS 30.0%
! GAGTTTTACATCAATTTTGG+GGG - Chr7:19705248-19705267 MsG0780037109.01.T01:CDS 30.0%
! GATTGGTCAAAATCAAACTA+GGG - Chr7:19705368-19705387 MsG0780037109.01.T01:CDS 30.0%
! TTTCAGAAAATTGGGTTTTC+TGG - Chr7:19705183-19705202 MsG0780037109.01.T01:CDS 30.0%
!!! GATTGTTGCTTTGAGTTTTT+GGG + Chr7:19704595-19704614 None:intergenic 30.0%
!!! TGATTGTTGCTTTGAGTTTT+TGG + Chr7:19704596-19704615 None:intergenic 30.0%
AACTAACATGATGACCTTGA+TGG + Chr7:19705053-19705072 None:intergenic 35.0%
AAGGAAGAGGAAGAAGAATA+AGG - Chr7:19704764-19704783 MsG0780037109.01.T01:CDS 35.0%
ACAATCACAACTTGCAAACA+TGG - Chr7:19704609-19704628 MsG0780037109.01.T01:CDS 35.0%
ACTAACATGATGACCTTGAT+GGG + Chr7:19705052-19705071 None:intergenic 35.0%
ACTCAGATTTATCAGCAGAT+TGG - Chr7:19705351-19705370 MsG0780037109.01.T01:CDS 35.0%
ATGCCAACAGGAACAAATTT+GGG - Chr7:19705213-19705232 MsG0780037109.01.T01:CDS 35.0%
CAATCACAACTTGCAAACAT+GGG - Chr7:19704610-19704629 MsG0780037109.01.T01:CDS 35.0%
CAGATGGTGAAACAATTCAA+TGG - Chr7:19704845-19704864 MsG0780037109.01.T01:CDS 35.0%
GAGTTAGGTCATAAATCAGA+TGG - Chr7:19704829-19704848 MsG0780037109.01.T01:CDS 35.0%
TATGCCAACAGGAACAAATT+TGG - Chr7:19705212-19705231 MsG0780037109.01.T01:CDS 35.0%
TTGTCACAAGATGGACATAT+TGG - Chr7:19705306-19705325 MsG0780037109.01.T01:CDS 35.0%
TTTCAACTCACAAGAGAGTT+AGG - Chr7:19704814-19704833 MsG0780037109.01.T01:CDS 35.0%
AACAGGAACAAATTTGGGAG+TGG - Chr7:19705218-19705237 MsG0780037109.01.T01:CDS 40.0%
ACAGGAACAAATTTGGGAGT+GGG - Chr7:19705219-19705238 MsG0780037109.01.T01:CDS 40.0%
ATTCTTGCTGCACATAAAGC+TGG + Chr7:19704793-19704812 None:intergenic 40.0%
CAAGGTTCATCTTTGTCTTC+TGG - Chr7:19704964-19704983 MsG0780037109.01.T01:CDS 40.0%
CACACAAAAGTTGAAGGAAG+AGG - Chr7:19704751-19704770 MsG0780037109.01.T01:CDS 40.0%
CATCAATCATCATCTTCTGG+TGG - Chr7:19705117-19705136 MsG0780037109.01.T01:CDS 40.0%
GAAGACAAAGATGAACCTTG+TGG + Chr7:19704964-19704983 None:intergenic 40.0%
GAATTAGGGTTGTCACAAGA+TGG - Chr7:19705297-19705316 MsG0780037109.01.T01:CDS 40.0%
GACAGACACACAAAAGTTGA+AGG - Chr7:19704745-19704764 MsG0780037109.01.T01:CDS 40.0%
GGTAATACTTTGACACCACA+AGG - Chr7:19704946-19704965 MsG0780037109.01.T01:CDS 40.0%
GGTTTAGTAGCTACTACTGT+TGG - Chr7:19705138-19705157 MsG0780037109.01.T01:CDS 40.0%
TCTGGATTTGATATGCCAAC+AGG - Chr7:19705201-19705220 MsG0780037109.01.T01:CDS 40.0%
TTATGGCCTCATCATCAAGT+TGG - Chr7:19705081-19705100 MsG0780037109.01.T01:CDS 40.0%
! ACTCCCAAATTTGTTCCTGT+TGG + Chr7:19705219-19705238 None:intergenic 40.0%
! CTGGTTTGCAGTTGAATGAT+AGG - Chr7:19704983-19705002 MsG0780037109.01.T01:CDS 40.0%
!! AGATGCCTGGTTTGGAATTA+GGG - Chr7:19705283-19705302 MsG0780037109.01.T01:CDS 40.0%
!! CATCATCAAGTTGGTGGATA+TGG - Chr7:19705090-19705109 MsG0780037109.01.T01:CDS 40.0%
!! TATCCACCAACTTGATGATG+AGG + Chr7:19705090-19705109 None:intergenic 40.0%
AAATCAGCAGATGCCTGGTT+TGG - Chr7:19705275-19705294 MsG0780037109.01.T01:CDS 45.0%
ATCATAGCTGCAACAGGAAC+AGG - Chr7:19704892-19704911 MsG0780037109.01.T01:CDS 45.0%
CCATCAATCATAGCTGCAAC+AGG - Chr7:19704886-19704905 MsG0780037109.01.T01:CDS 45.0%
CCTGTTGCAGCTATGATTGA+TGG + Chr7:19704889-19704908 None:intergenic 45.0%
GAACAAATTTGGGAGTGGGA+GGG - Chr7:19705223-19705242 MsG0780037109.01.T01:CDS 45.0%
GAAGAAGCTAAAGCTGAAGC+TGG + Chr7:19704925-19704944 None:intergenic 45.0%
GACAACCCTAATTCCAAACC+AGG + Chr7:19705291-19705310 None:intergenic 45.0%
GGGAGAGAACAAAGAATCAG+AGG - Chr7:19704630-19704649 MsG0780037109.01.T01:CDS 45.0%
! GCTTCAGCTTTAGCTTCTTC+TGG - Chr7:19704925-19704944 MsG0780037109.01.T01:CDS 45.0%
!! CAGATGCCTGGTTTGGAATT+AGG - Chr7:19705282-19705301 MsG0780037109.01.T01:CDS 45.0%
GGAACAAATTTGGGAGTGGG+AGG - Chr7:19705222-19705241 MsG0780037109.01.T01:CDS 50.0%
! ATGACCTTGATGGGCCTGAT+GGG + Chr7:19705043-19705062 None:intergenic 50.0%
!! TGGCCTCATCATCAAGTTGG+TGG - Chr7:19705084-19705103 MsG0780037109.01.T01:CDS 50.0%
GGTGCAAATCAGCAGATGCC+TGG - Chr7:19705270-19705289 MsG0780037109.01.T01:CDS 55.0%
! GATGACCTTGATGGGCCTGA+TGG + Chr7:19705044-19705063 None:intergenic 55.0%
TCAGACCCATCAAGCCCACC+AGG - Chr7:19705017-19705036 MsG0780037109.01.T01:CDS 60.0%
!! ATGGGCCTGGTGGGCTTGAT+GGG + Chr7:19705025-19705044 None:intergenic 60.0%
CAGGCCCATCAGGCCCATCA+AGG - Chr7:19705036-19705055 MsG0780037109.01.T01:CDS 65.0%
TCAAGCCCACCAGGCCCATC+AGG - Chr7:19705026-19705045 MsG0780037109.01.T01:CDS 65.0%
! ATGGGCCTGATGGGCCTGGT+GGG + Chr7:19705034-19705053 None:intergenic 65.0%
! CTTGATGGGCCTGATGGGCC+TGG + Chr7:19705038-19705057 None:intergenic 65.0%
!! GATGGGCCTGGTGGGCTTGA+TGG + Chr7:19705026-19705045 None:intergenic 65.0%
! GATGGGCCTGATGGGCCTGG+TGG + Chr7:19705035-19705054 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 19704586 19705455 19704586 ID=MsG0780037109.01;Name=MsG0780037109.01
Chr7 mRNA 19704586 19705455 19704586 ID=MsG0780037109.01.T01;Parent=MsG0780037109.01;Name=MsG0780037109.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|289
Chr7 exon 19704586 19705455 19704586 ID=MsG0780037109.01.T01:exon:3879;Parent=MsG0780037109.01.T01
Chr7 CDS 19704586 19705455 19704586 ID=MsG0780037109.01.T01:cds;Parent=MsG0780037109.01.T01
Gene Sequence

>MsG0780037109.01.T01

ATGGATCCCAAAAACTCAAAGCAACAATCACAACTTGCAAACATGGGAGAGAACAAAGAATCAGAGGCAAAAAATCTTCAAATTGTGTTATCTGAAACAACAACAACAACAAAAGATGAAACAAAGAAACAACTAGCACCAAAAAGAACATCAAACAAAGACAGACACACAAAAGTTGAAGGAAGAGGAAGAAGAATAAGGATGCCAGCTTTATGTGCAGCAAGAATCTTTCAACTCACAAGAGAGTTAGGTCATAAATCAGATGGTGAAACAATTCAATGGCTTTTACAACAATCTGAACCATCAATCATAGCTGCAACAGGAACAGGAACAATACCAGCTTCAGCTTTAGCTTCTTCTGGTAATACTTTGACACCACAAGGTTCATCTTTGTCTTCTGGTTTGCAGTTGAATGATAGGAATAATTGGGCTCAGACCCATCAAGCCCACCAGGCCCATCAGGCCCATCAAGGTCATCATGTTAGTTCTACAAGTTTATGGCCTCATCATCAAGTTGGTGGATATGGATTTCATCAATCATCATCTTCTGGTGGTTTAGTAGCTACTACTGTTGGTGAAAATAATAGTGGAAATTATTTTCAGAAAATTGGGTTTTCTGGATTTGATATGCCAACAGGAACAAATTTGGGAGTGGGAGGGATGAGTTTTACATCAATTTTGGGGGGTGCAAATCAGCAGATGCCTGGTTTGGAATTAGGGTTGTCACAAGATGGACATATTGGTGTGTTGAATCAACAAGCTTTGACTCAGATTTATCAGCAGATTGGTCAAAATCAAACTAGGGTTCAGCACCAGAATCAGCAGAATAATAATACTCCTAAAGATGATTCTCACAATTCAGAACAGTAG

Protein sequence

>MsG0780037109.01.T01

MDPKNSKQQSQLANMGENKESEAKNLQIVLSETTTTTKDETKKQLAPKRTSNKDRHTKVEGRGRRIRMPALCAARIFQLTRELGHKSDGETIQWLLQQSEPSIIAATGTGTIPASALASSGNTLTPQGSSLSSGLQLNDRNNWAQTHQAHQAHQAHQGHHVSSTSLWPHHQVGGYGFHQSSSSGGLVATTVGENNSGNYFQKIGFSGFDMPTGTNLGVGGMSFTSILGGANQQMPGLELGLSQDGHIGVLNQQALTQIYQQIGQNQTRVQHQNQQNNNTPKDDSHNSEQ*