AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007208.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007208.01.T01 MTR_2g022610 95.098 102 5 0 1 102 1 102 1.99e-67 198
MsG0280007208.01.T01 MTR_4g131720 70.588 102 29 1 1 101 1 102 3.07e-49 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007208.01.T01 AT3G06070 39.560 91 50 3 1 86 1 91 9.81e-15 65.9
MsG0280007208.01.T01 AT5G19190 38.095 105 57 4 1 101 1 101 1.74e-14 65.1

Find 58 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGAAACTAAATACAATTT+TGG 0.181446 2:+11948297 MsG0280007208.01.T01:five_prime_UTR
CTTCATCTTCATCCATTATT+AGG 0.195790 2:-11948416 None:intergenic
AACTAAACTTCCACAAATTA+TGG 0.212788 2:+11948231 MsG0280007208.01.T01:five_prime_UTR
TCCTTTCTGTACCTCCTTCC+TGG 0.231431 2:-11948918 None:intergenic
ATTAGGTGTTATATAATATA+TGG 0.247342 2:-11949211 None:intergenic
TTAATATCATTAACAGAAAA+AGG 0.277524 2:-11949333 None:intergenic
TAATTAATTCTTTAGCCTTT+AGG 0.283220 2:+11949123 MsG0280007208.01.T01:three_prime_UTR
ATGGGTGAGTTAAGACATAT+TGG 0.306508 2:-11948186 None:intergenic
TATACATGATGACTAATATT+AGG 0.313703 2:-11949228 None:intergenic
AGAAATTGCTCTCCTAATAA+TGG 0.320698 2:+11948404 MsG0280007208.01.T01:CDS
GAGTGAGTACACTTATTAAT+TGG 0.322435 2:-11947993 None:intergenic
TCAACCTGCAAACTCTCTTC+AGG 0.344380 2:+11948452 MsG0280007208.01.T01:CDS
AATTAACATGGAGGTTGCTT+TGG 0.348717 2:+11948344 MsG0280007208.01.T01:exon
CTTCTTGTGTCCATAATTTG+TGG 0.353670 2:-11948241 None:intergenic
TTCCTGGTTGTGTTGCTTGT+GGG 0.361313 2:-11948902 None:intergenic
CATGGAGGTTGCTTTGGAAA+TGG 0.372075 2:+11948350 MsG0280007208.01.T01:exon
GTTTGATAGAATTCTGTGTT+AGG 0.383033 2:-11949004 None:intergenic
GCCTTTAGGCAATAATTGTA+TGG 0.399792 2:+11949137 MsG0280007208.01.T01:three_prime_UTR
AGAAGAGAAAGCAAAGGAAC+AGG 0.411669 2:+11948870 MsG0280007208.01.T01:CDS
CTTCCTGGTTGTGTTGCTTG+TGG 0.428280 2:-11948903 None:intergenic
AGAACCTGAAGAGAGTTTGC+AGG 0.436707 2:-11948456 None:intergenic
AGTGGAGGTTGCAAAACCTT+TGG 0.437819 2:-11949262 None:intergenic
TGGGTGGTCATGTGGGTTAG+TGG 0.454647 2:-11948166 None:intergenic
TAATATTAGTCATCATGTAT+AGG 0.461723 2:+11949230 MsG0280007208.01.T01:three_prime_UTR
AAATCACACAGCTTTATAGA+AGG 0.463158 2:-11949053 None:intergenic
AATATTTCCATGATACTTGT+GGG 0.469911 2:+11948108 MsG0280007208.01.T01:five_prime_UTR
GGGTGGTCATGTGGGTTAGT+GGG 0.473239 2:-11948165 None:intergenic
AATTCTATCAAACAGTGAAA+TGG 0.476428 2:+11949014 MsG0280007208.01.T01:three_prime_UTR
CACAGTTGCTTAGCTAGCTA+TGG 0.483960 2:-11948133 None:intergenic
TAGCTAAGCAACTGTGACCA+TGG 0.485012 2:+11948140 MsG0280007208.01.T01:five_prime_UTR
GACATATTGGGTGGTCATGT+GGG 0.515223 2:-11948173 None:intergenic
GCCATACAATTATTGCCTAA+AGG 0.516035 2:-11949138 None:intergenic
TAATATTTCCATGATACTTG+TGG 0.519213 2:+11948107 MsG0280007208.01.T01:five_prime_UTR
TATAAAGGTGGATATGTACA+TGG 0.524484 2:+11949183 MsG0280007208.01.T01:three_prime_UTR
TGGGTGAGTTAAGACATATT+GGG 0.527268 2:-11948185 None:intergenic
AAGAAATCAAACTGTGGAGG+AGG 0.531737 2:-11948828 None:intergenic
AGACATATTGGGTGGTCATG+TGG 0.535069 2:-11948174 None:intergenic
TATGGACCCCACAAGTATCA+TGG 0.544735 2:-11948115 None:intergenic
AGCTAAGCAACTGTGACCAT+GGG 0.549449 2:+11948141 MsG0280007208.01.T01:five_prime_UTR
ATGTGGGTTAGTGGGACCCA+TGG 0.555372 2:-11948157 None:intergenic
ACCAGGAAGGAGGTACAGAA+AGG 0.556139 2:+11948917 MsG0280007208.01.T01:CDS
ATCCCACAAGCAACACAACC+AGG 0.573882 2:+11948900 MsG0280007208.01.T01:CDS
GTATAGGAGGATTCAACCAA+AGG 0.585124 2:+11949246 MsG0280007208.01.T01:three_prime_UTR
GATCAGATATCATCTGGACA+TGG 0.589069 2:+11948035 MsG0280007208.01.T01:five_prime_UTR
TATTAGTCATCATGTATAGG+AGG 0.591401 2:+11949233 MsG0280007208.01.T01:three_prime_UTR
CACAAGCAACACAACCAGGA+AGG 0.608653 2:+11948904 MsG0280007208.01.T01:CDS
TTGATTAGAAGAGAAAGCAA+AGG 0.610534 2:+11948864 MsG0280007208.01.T01:CDS
TTTGCAGGTTGAGAAGCAAG+TGG 0.620205 2:-11948441 None:intergenic
ATAAAGGTGGATATGTACAT+GGG 0.623401 2:+11949184 MsG0280007208.01.T01:three_prime_UTR
TAGAAGAAATCAAACTGTGG+AGG 0.634181 2:-11948831 None:intergenic
ATCAGATATCATCTGGACAT+GGG 0.638660 2:+11948036 MsG0280007208.01.T01:five_prime_UTR
AAGCAACACAACCAGGAAGG+AGG 0.653987 2:+11948907 MsG0280007208.01.T01:CDS
ATATTTCCATGATACTTGTG+GGG 0.660564 2:+11948109 MsG0280007208.01.T01:five_prime_UTR
TTTATATTGAATTAACATGG+AGG 0.678774 2:+11948335 MsG0280007208.01.T01:exon
AATGCTTGTCCACACAGTGG+AGG 0.692722 2:-11949277 None:intergenic
GTGAGTTAAGACATATTGGG+TGG 0.706648 2:-11948182 None:intergenic
CAAAATGCTTGTCCACACAG+TGG 0.720567 2:-11949280 None:intergenic
TCATAGAAGAAATCAAACTG+TGG 0.762193 2:-11948834 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTCTATTTTATTATATAA+AGG + Chr2:11949168-11949187 MsG0280007208.01.T01:three_prime_UTR 10.0%
!!! TTAGTTTTATATAAAGAAAA+AGG - Chr2:11948217-11948236 None:intergenic 10.0%
!! TTAATATCATTAACAGAAAA+AGG - Chr2:11949336-11949355 None:intergenic 15.0%
!! TTTATATAAAGAAAAAGGTA+AGG - Chr2:11948212-11948231 None:intergenic 15.0%
!!! ATTAGGTGTTATATAATATA+TGG - Chr2:11949214-11949233 None:intergenic 15.0%
!!! TCTATTTTATTATATAAAGG+TGG + Chr2:11949171-11949190 MsG0280007208.01.T01:three_prime_UTR 15.0%
!!! TCTTTTATATTGAATTAACA+TGG + Chr2:11948332-11948351 MsG0280007208.01.T01:exon 15.0%
!!! TCTTTTTAATCAATCAATTT+AGG + Chr2:11948083-11948102 MsG0280007208.01.T01:five_prime_UTR 15.0%
!! AAAATGTCATAATTGAAACT+TGG - Chr2:11948571-11948590 None:intergenic 20.0%
!! TAATATTAGTCATCATGTAT+AGG + Chr2:11949230-11949249 MsG0280007208.01.T01:three_prime_UTR 20.0%
!! TATACATGATGACTAATATT+AGG - Chr2:11949231-11949250 None:intergenic 20.0%
!! TATATTAGTATGTAACTATG+AGG - Chr2:11948516-11948535 None:intergenic 20.0%
!! TTAAAGAATTTATTTGTGCA+TGG + Chr2:11948593-11948612 MsG0280007208.01.T01:intron 20.0%
!! TTTATATTGAATTAACATGG+AGG + Chr2:11948335-11948354 MsG0280007208.01.T01:exon 20.0%
!!! CTAGAAACTAAATACAATTT+TGG + Chr2:11948297-11948316 MsG0280007208.01.T01:five_prime_UTR 20.0%
!!! TAATTAATTCTTTAGCCTTT+AGG + Chr2:11949123-11949142 MsG0280007208.01.T01:three_prime_UTR 20.0%
! AAAAAGATGTGAAAAAACAG+CGG - Chr2:11948070-11948089 None:intergenic 25.0%
! AACTAAACTTCCACAAATTA+TGG + Chr2:11948231-11948250 MsG0280007208.01.T01:five_prime_UTR 25.0%
! AATATTTCCATGATACTTGT+GGG + Chr2:11948108-11948127 MsG0280007208.01.T01:five_prime_UTR 25.0%
! AATTCTATCAAACAGTGAAA+TGG + Chr2:11949014-11949033 MsG0280007208.01.T01:three_prime_UTR 25.0%
! ATAAAGAAAAAGGTAAGGAT+GGG - Chr2:11948207-11948226 None:intergenic 25.0%
! TAATATTTCCATGATACTTG+TGG + Chr2:11948107-11948126 MsG0280007208.01.T01:five_prime_UTR 25.0%
! TATAAAGAAAAAGGTAAGGA+TGG - Chr2:11948208-11948227 None:intergenic 25.0%
! TATTTGCATATAGAGAAAGA+AGG - Chr2:11948721-11948740 None:intergenic 25.0%
AAAAGATGTGAAAAAACAGC+GGG - Chr2:11948069-11948088 None:intergenic 30.0%
AGAAATTGCTCTCCTAATAA+TGG + Chr2:11948404-11948423 MsG0280007208.01.T01:CDS 30.0%
ATAAAGGTGGATATGTACAT+GGG + Chr2:11949184-11949203 MsG0280007208.01.T01:three_prime_UTR 30.0%
ATATTTCCATGATACTTGTG+GGG + Chr2:11948109-11948128 MsG0280007208.01.T01:five_prime_UTR 30.0%
CATAATTGAAACTTGGAAGA+AGG - Chr2:11948564-11948583 None:intergenic 30.0%
CTTCATCTTCATCCATTATT+AGG - Chr2:11948419-11948438 None:intergenic 30.0%
GAGGTGCATGTATTTAATTA+AGG - Chr2:11948497-11948516 None:intergenic 30.0%
GAGTGAGTACACTTATTAAT+TGG - Chr2:11947996-11948015 None:intergenic 30.0%
GTGCATGTATTTAATTAAGG+AGG - Chr2:11948494-11948513 None:intergenic 30.0%
GTTTGATAGAATTCTGTGTT+AGG - Chr2:11949007-11949026 None:intergenic 30.0%
TATAAAGGTGGATATGTACA+TGG + Chr2:11949183-11949202 MsG0280007208.01.T01:three_prime_UTR 30.0%
TATTAGTCATCATGTATAGG+AGG + Chr2:11949233-11949252 MsG0280007208.01.T01:three_prime_UTR 30.0%
TCATAGAAGAAATCAAACTG+TGG - Chr2:11948837-11948856 None:intergenic 30.0%
TTGATTAGAAGAGAAAGCAA+AGG + Chr2:11948864-11948883 MsG0280007208.01.T01:CDS 30.0%
! AAATCACACAGCTTTATAGA+AGG - Chr2:11949056-11949075 None:intergenic 30.0%
! CTCTCTTGAGATTGAATTTT+TGG + Chr2:11948013-11948032 MsG0280007208.01.T01:five_prime_UTR 30.0%
! TTTTTGGATCAGATATCATC+TGG + Chr2:11948029-11948048 MsG0280007208.01.T01:five_prime_UTR 30.0%
AATTAACATGGAGGTTGCTT+TGG + Chr2:11948344-11948363 MsG0280007208.01.T01:exon 35.0%
ATCAGATATCATCTGGACAT+GGG + Chr2:11948036-11948055 MsG0280007208.01.T01:five_prime_UTR 35.0%
ATGGGTGAGTTAAGACATAT+TGG - Chr2:11948189-11948208 None:intergenic 35.0%
CTTCTTGTGTCCATAATTTG+TGG - Chr2:11948244-11948263 None:intergenic 35.0%
GCCATACAATTATTGCCTAA+AGG - Chr2:11949141-11949160 None:intergenic 35.0%
GCCTTTAGGCAATAATTGTA+TGG + Chr2:11949137-11949156 MsG0280007208.01.T01:three_prime_UTR 35.0%
TAGAAGAAATCAAACTGTGG+AGG - Chr2:11948834-11948853 None:intergenic 35.0%
TGGGTGAGTTAAGACATATT+GGG - Chr2:11948188-11948207 None:intergenic 35.0%
TTTATTTGTGCATGGCAGTT+CGG + Chr2:11948601-11948620 MsG0280007208.01.T01:intron 35.0%
AAGAAATCAAACTGTGGAGG+AGG - Chr2:11948831-11948850 None:intergenic 40.0%
AGAAGAGAAAGCAAAGGAAC+AGG + Chr2:11948870-11948889 MsG0280007208.01.T01:CDS 40.0%
GATCAGATATCATCTGGACA+TGG + Chr2:11948035-11948054 MsG0280007208.01.T01:five_prime_UTR 40.0%
GTATAGGAGGATTCAACCAA+AGG + Chr2:11949246-11949265 MsG0280007208.01.T01:three_prime_UTR 40.0%
GTGAGTTAAGACATATTGGG+TGG - Chr2:11948185-11948204 None:intergenic 40.0%
AGAACCTGAAGAGAGTTTGC+AGG - Chr2:11948459-11948478 None:intergenic 45.0%
AGACATATTGGGTGGTCATG+TGG - Chr2:11948177-11948196 None:intergenic 45.0%
AGCTAAGCAACTGTGACCAT+GGG + Chr2:11948141-11948160 MsG0280007208.01.T01:five_prime_UTR 45.0%
AGTGGAGGTTGCAAAACCTT+TGG - Chr2:11949265-11949284 None:intergenic 45.0%
CAAAATGCTTGTCCACACAG+TGG - Chr2:11949283-11949302 None:intergenic 45.0%
CACAGTTGCTTAGCTAGCTA+TGG - Chr2:11948136-11948155 None:intergenic 45.0%
GACATATTGGGTGGTCATGT+GGG - Chr2:11948176-11948195 None:intergenic 45.0%
TAGCTAAGCAACTGTGACCA+TGG + Chr2:11948140-11948159 MsG0280007208.01.T01:five_prime_UTR 45.0%
TATGGACCCCACAAGTATCA+TGG - Chr2:11948118-11948137 None:intergenic 45.0%
TCAACCTGCAAACTCTCTTC+AGG + Chr2:11948452-11948471 MsG0280007208.01.T01:CDS 45.0%
! CTGTGTGGACAAGCATTTTG+TGG + Chr2:11949283-11949302 MsG0280007208.01.T01:three_prime_UTR 45.0%
! TTTGCAGGTTGAGAAGCAAG+TGG - Chr2:11948444-11948463 None:intergenic 45.0%
!! CATGGAGGTTGCTTTGGAAA+TGG + Chr2:11948350-11948369 MsG0280007208.01.T01:exon 45.0%
!! TTCCTGGTTGTGTTGCTTGT+GGG - Chr2:11948905-11948924 None:intergenic 45.0%
!!! TTAAATTAATTTTATTTTGT+AGG + Chr2:11948785-11948804 MsG0280007208.01.T01:intron 5.0%
AAGCAACACAACCAGGAAGG+AGG + Chr2:11948907-11948926 MsG0280007208.01.T01:CDS 50.0%
AATGCTTGTCCACACAGTGG+AGG - Chr2:11949280-11949299 None:intergenic 50.0%
ATCCCACAAGCAACACAACC+AGG + Chr2:11948900-11948919 MsG0280007208.01.T01:CDS 50.0%
ATTTGTGCATGGCAGTTCGG+TGG + Chr2:11948604-11948623 MsG0280007208.01.T01:intron 50.0%
CACAAGCAACACAACCAGGA+AGG + Chr2:11948904-11948923 MsG0280007208.01.T01:CDS 50.0%
TCCTTTCTGTACCTCCTTCC+TGG - Chr2:11948921-11948940 None:intergenic 50.0%
! ACCAGGAAGGAGGTACAGAA+AGG + Chr2:11948917-11948936 MsG0280007208.01.T01:CDS 50.0%
! GTTTTGCAACCTCCACTGTG+TGG + Chr2:11949268-11949287 MsG0280007208.01.T01:three_prime_UTR 50.0%
!! CTTCCTGGTTGTGTTGCTTG+TGG - Chr2:11948906-11948925 None:intergenic 50.0%
!! ATGTGGGTTAGTGGGACCCA+TGG - Chr2:11948160-11948179 None:intergenic 55.0%
!! GGGTGGTCATGTGGGTTAGT+GGG - Chr2:11948168-11948187 None:intergenic 55.0%
!! TGGGTGGTCATGTGGGTTAG+TGG - Chr2:11948169-11948188 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 11947984 11949368 11947984 ID=MsG0280007208.01;Name=MsG0280007208.01
Chr2 mRNA 11947984 11949368 11947984 ID=MsG0280007208.01.T01;Parent=MsG0280007208.01;Name=MsG0280007208.01.T01;_AED=0.46;_eAED=0.46;_QI=367|1|1|1|0|0|2|376|102
Chr2 exon 11947984 11948473 11947984 ID=MsG0280007208.01.T01:exon:2777;Parent=MsG0280007208.01.T01
Chr2 exon 11948807 11949368 11948807 ID=MsG0280007208.01.T01:exon:2778;Parent=MsG0280007208.01.T01
Chr2 five_prime_UTR 11947984 11948350 11947984 ID=MsG0280007208.01.T01:five_prime_utr;Parent=MsG0280007208.01.T01
Chr2 CDS 11948351 11948473 11948351 ID=MsG0280007208.01.T01:cds;Parent=MsG0280007208.01.T01
Chr2 CDS 11948807 11948992 11948807 ID=MsG0280007208.01.T01:cds;Parent=MsG0280007208.01.T01
Chr2 three_prime_UTR 11948993 11949368 11948993 ID=MsG0280007208.01.T01:three_prime_utr;Parent=MsG0280007208.01.T01
Gene Sequence

>MsG0280007208.01.T01

ATGGAGGTTGCTTTGGAAATGGAAGATGATTTATTTTTTGCAAATTTGAGCAAAGAAATTGCTCTCCTAATAATGGATGAAGATGAAGATCCACTTGCTTCTCAACCTGCAAACTCTCTTCAGGATTTCTCTAGAGCAATTCATCCTCCTCCACAGTTTGATTTCTTCTATGAGCAAGCTTTGATTAGAAGAGAAAGCAAAGGAACAGGAGTGTTTATCCCACAAGCAACACAACCAGGAAGGAGGTACAGAAAGGAAGAATCTAATTCATTCTCAAAATATCAAAAGCAGTCTCAAGACACAAGATGA

Protein sequence

>MsG0280007208.01.T01

MEVALEMEDDLFFANLSKEIALLIMDEDEDPLASQPANSLQDFSRAIHPPPQFDFFYEQALIRRESKGTGVFIPQATQPGRRYRKEESNSFSKYQKQSQDTR*