Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029592.01.T01 | XP_003617202.1 | 89.157 | 83 | 9 | 0 | 7 | 89 | 158 | 240 | 4.16E-44 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029592.01.T01 | G7KCU0 | 89.157 | 83 | 9 | 0 | 7 | 89 | 158 | 240 | 1.99e-44 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0580029592.01 | 0.812463 | 4.186844e-51 | 2.663985e-48 |
| MsG0080048461.01 | MsG0580029592.01 | 0.800103 | 1.685841e-48 | 7.783318e-46 |
| MsG0180000001.01 | MsG0580029592.01 | 0.818350 | 2.051739e-52 | 1.532206e-49 |
| MsG0180000197.01 | MsG0580029592.01 | 0.804104 | 2.535514e-49 | 1.296145e-46 |
| MsG0180001117.01 | MsG0580029592.01 | 0.805692 | 1.180734e-49 | 6.287916e-47 |
| MsG0180001566.01 | MsG0580029592.01 | 0.817157 | 3.814710e-52 | 2.756786e-49 |
| MsG0180002273.01 | MsG0580029592.01 | 0.806766 | 7.012612e-50 | 3.838379e-47 |
| MsG0180003586.01 | MsG0580029592.01 | 0.813811 | 2.118752e-51 | 1.397742e-48 |
| MsG0180004272.01 | MsG0580029592.01 | 0.814749 | 1.314606e-51 | 8.895145e-49 |
| MsG0180004486.01 | MsG0580029592.01 | 0.841284 | 5.215641e-58 | 7.626321e-55 |
| MsG0180004689.01 | MsG0580029592.01 | 0.807783 | 4.270505e-50 | 2.400029e-47 |
| MsG0180005443.01 | MsG0580029592.01 | 0.828950 | 6.760866e-55 | 6.820535e-52 |
| MsG0180005445.01 | MsG0580029592.01 | 0.811036 | 8.559475e-51 | 5.242690e-48 |
| MsG0180005451.01 | MsG0580029592.01 | 0.825831 | 3.781517e-54 | 3.485722e-51 |
| MsG0180005493.01 | MsG0580029592.01 | 0.868543 | 6.040206e-66 | 2.193442e-62 |
| MsG0180005507.01 | MsG0580029592.01 | 0.803540 | 3.320811e-49 | 1.673224e-46 |
| MsG0180006025.01 | MsG0580029592.01 | 0.816203 | 6.241466e-52 | 4.394411e-49 |
| MsG0280010632.01 | MsG0580029592.01 | 0.804648 | 1.953375e-49 | 1.012637e-46 |
| MsG0280011070.01 | MsG0580029592.01 | 0.801470 | 8.870382e-49 | 4.239534e-46 |
| MsG0280011334.01 | MsG0580029592.01 | 0.813224 | 2.852440e-51 | 1.851822e-48 |
| MsG0380011729.01 | MsG0580029592.01 | 0.809574 | 1.769433e-50 | 1.042675e-47 |
| MsG0380012057.01 | MsG0580029592.01 | 0.822539 | 2.244088e-53 | 1.883834e-50 |
| MsG0480020242.01 | MsG0580029592.01 | 0.816111 | 6.544914e-52 | 4.596344e-49 |
| MsG0480020331.01 | MsG0580029592.01 | 0.813707 | 2.233310e-51 | 1.469159e-48 |
| MsG0480021870.01 | MsG0580029592.01 | 0.815458 | 9.151066e-52 | 6.312898e-49 |
| MsG0480021896.01 | MsG0580029592.01 | -0.802994 | 4.306981e-49 | 2.139753e-46 |
| MsG0480021900.01 | MsG0580029592.01 | 0.821317 | 4.306043e-53 | 3.491369e-50 |
| MsG0480022115.01 | MsG0580029592.01 | 0.827822 | 1.264930e-54 | 1.234859e-51 |
| MsG0480022228.01 | MsG0580029592.01 | 0.824273 | 8.826832e-54 | 7.781030e-51 |
| MsG0480022597.01 | MsG0580029592.01 | 0.802260 | 6.104275e-49 | 2.976635e-46 |
| MsG0480023272.01 | MsG0580029592.01 | 0.800724 | 1.260097e-48 | 5.909983e-46 |
| MsG0480023377.01 | MsG0580029592.01 | 0.800575 | 1.351587e-48 | 6.315234e-46 |
| MsG0480023451.01 | MsG0580029592.01 | 0.812499 | 4.110593e-51 | 2.617879e-48 |
| MsG0480023670.01 | MsG0580029592.01 | 0.825573 | 4.355808e-54 | 3.985409e-51 |
| MsG0480023718.01 | MsG0580029592.01 | 0.826528 | 2.581352e-54 | 2.426993e-51 |
| MsG0580024157.01 | MsG0580029592.01 | 0.826847 | 2.166691e-54 | 2.055863e-51 |
| MsG0580024418.01 | MsG0580029592.01 | 0.816692 | 4.851228e-52 | 3.461299e-49 |
| MsG0580024421.01 | MsG0580029592.01 | 0.806278 | 8.891948e-50 | 4.806687e-47 |
| MsG0580024960.01 | MsG0580029592.01 | 0.808198 | 3.483804e-50 | 1.979531e-47 |
| MsG0580025008.01 | MsG0580029592.01 | 0.826697 | 2.352811e-54 | 2.222838e-51 |
| MsG0580025025.01 | MsG0580029592.01 | 0.805741 | 1.153158e-49 | 6.148872e-47 |
| MsG0580025206.01 | MsG0580029592.01 | 0.825085 | 5.681253e-54 | 5.126892e-51 |
| MsG0380015289.01 | MsG0580029592.01 | 0.812380 | 4.365464e-51 | 2.771341e-48 |
| MsG0380015702.01 | MsG0580029592.01 | 0.805424 | 1.344105e-49 | 7.108303e-47 |
| MsG0380016510.01 | MsG0580029592.01 | 0.812023 | 5.221713e-51 | 3.282941e-48 |
| MsG0380016985.01 | MsG0580029592.01 | 0.829356 | 5.388790e-55 | 5.502746e-52 |
| MsG0380017055.01 | MsG0580029592.01 | 0.808477 | 3.038797e-50 | 1.739320e-47 |
| MsG0380017232.01 | MsG0580029592.01 | 0.800420 | 1.453732e-48 | 6.766111e-46 |
| MsG0580028517.01 | MsG0580029592.01 | 0.823374 | 1.433498e-53 | 1.231957e-50 |
| MsG0580028592.01 | MsG0580029592.01 | 0.801491 | 8.782741e-49 | 4.199967e-46 |
| MsG0580029592.01 | MsG0680030447.01 | 0.817663 | 2.934292e-52 | 2.150120e-49 |
| MsG0580029592.01 | MsG0780036630.01 | 0.818730 | 1.683305e-52 | 1.270165e-49 |
| MsG0580029592.01 | MsG0780038937.01 | 0.814245 | 1.699631e-51 | 1.134505e-48 |
| MsG0580029592.01 | MsG0780040176.01 | 0.817565 | 3.087466e-52 | 2.256238e-49 |
| MsG0580029592.01 | MsG0780040418.01 | 0.807202 | 5.671301e-50 | 3.139548e-47 |
| MsG0580029592.01 | MsG0780040947.01 | 0.809343 | 1.983459e-50 | 1.161633e-47 |
| MsG0580029592.01 | MsG0880042829.01 | 0.819169 | 1.337084e-52 | 1.021248e-49 |
| MsG0580029592.01 | MsG0880043194.01 | 0.821492 | 3.923473e-53 | 3.196696e-50 |
| MsG0580029592.01 | MsG0880043489.01 | 0.817625 | 2.992084e-52 | 2.190152e-49 |
| MsG0580029592.01 | MsG0880043804.01 | 0.815567 | 8.651323e-52 | 5.985921e-49 |
| MsG0580029592.01 | MsG0880044075.01 | 0.809574 | 1.769433e-50 | 1.042675e-47 |
| MsG0580029592.01 | MsG0880045897.01 | -0.822013 | 2.972056e-53 | 2.457489e-50 |
| MsG0580029592.01 | MsG0880046027.01 | 0.831971 | 1.233661e-55 | 1.360092e-52 |
| MsG0580029592.01 | MsG0880046560.01 | 0.809124 | 2.209450e-50 | 1.286435e-47 |
| MsG0580029592.01 | MsG0880046561.01 | 0.828171 | 1.042796e-54 | 1.028473e-51 |
| MsG0580029592.01 | MsG0880046762.01 | 0.810951 | 8.926171e-51 | 5.455436e-48 |
| MsG0280006692.01 | MsG0580029592.01 | 0.860917 | 1.478489e-63 | 4.108374e-60 |
| MsG0280007048.01 | MsG0580029592.01 | 0.801090 | 1.060895e-48 | 5.022508e-46 |
| MsG0280007208.01 | MsG0580029592.01 | 0.843414 | 1.422157e-58 | 2.223529e-55 |
| MsG0280007484.01 | MsG0580029592.01 | 0.852194 | 5.417896e-61 | 1.123366e-57 |
| MsG0280007710.01 | MsG0580029592.01 | 0.800628 | 1.318434e-48 | 6.168656e-46 |
| MsG0280010389.01 | MsG0580029592.01 | 0.801453 | 8.941895e-49 | 4.271896e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029592.01.T01 | MTR_5g088990 | 89.157 | 83 | 9 | 0 | 7 | 89 | 158 | 240 | 5.04e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACTCATCGTCACCGGAAAT+TGG | 0.237543 | 5:-99035724 | None:intergenic |
| AAATTTCACAACATGTTATA+TGG | 0.271985 | 5:+99035871 | MsG0580029592.01.T01:CDS |
| TTCCTTAGGATGGTTGAAAT+TGG | 0.295594 | 5:-99035770 | None:intergenic |
| TCTCCGGCTAGTCCAATTTC+CGG | 0.340374 | 5:+99035712 | MsG0580029592.01.T01:CDS |
| AGCCGGAGACGGAGAATCTT+CGG | 0.389032 | 5:-99035698 | None:intergenic |
| TCATCGTCACTGTCTTCCTT+AGG | 0.390911 | 5:-99035784 | None:intergenic |
| CTTTAACATCGAAACAGAAC+TGG | 0.404508 | 5:-99035842 | None:intergenic |
| TTGGAAGGAATATTTGTGTC+TGG | 0.465359 | 5:-99035751 | None:intergenic |
| TGACAAAGATAAAGATAAAG+AGG | 0.482717 | 5:+99035816 | MsG0580029592.01.T01:CDS |
| CGTCACTGTCTTCCTTAGGA+TGG | 0.511800 | 5:-99035780 | None:intergenic |
| TTAGGATGGTTGAAATTGGA+AGG | 0.515170 | 5:-99035766 | None:intergenic |
| TTCCAATTTCAACCATCCTA+AGG | 0.526093 | 5:+99035768 | MsG0580029592.01.T01:CDS |
| ATCCGAAGATTCTCCGTCTC+CGG | 0.529783 | 5:+99035696 | MsG0580029592.01.T01:CDS |
| TCACCGGAAATTGGACTAGC+CGG | 0.571076 | 5:-99035715 | None:intergenic |
| TGGCGCAGACTCATCGTCAC+CGG | 0.575216 | 5:-99035731 | None:intergenic |
| GAAATTGGACTAGCCGGAGA+CGG | 0.643836 | 5:-99035709 | None:intergenic |
| ATAAATGTGACTCATGGACA+AGG | 0.658209 | 5:+99035648 | None:intergenic |
| GGAGACGGAGAATCTTCGGA+TGG | 0.673058 | 5:-99035694 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTCACAACATGTTATA+TGG | + | Chr5:99035871-99035890 | MsG0580029592.01.T01:CDS | 20.0% |
| ! | TGACAAAGATAAAGATAAAG+AGG | + | Chr5:99035816-99035835 | MsG0580029592.01.T01:CDS | 25.0% |
| !!! | ATGGATGTCTTGTTTTATTA+GGG | + | Chr5:99035890-99035909 | MsG0580029592.01.T01:CDS | 25.0% |
| !!! | TATGGATGTCTTGTTTTATT+AGG | + | Chr5:99035889-99035908 | MsG0580029592.01.T01:CDS | 25.0% |
| CTTTAACATCGAAACAGAAC+TGG | - | Chr5:99035845-99035864 | None:intergenic | 35.0% | |
| TTAGGATGGTTGAAATTGGA+AGG | - | Chr5:99035769-99035788 | None:intergenic | 35.0% | |
| TTCCAATTTCAACCATCCTA+AGG | + | Chr5:99035768-99035787 | MsG0580029592.01.T01:CDS | 35.0% | |
| TTCCTTAGGATGGTTGAAAT+TGG | - | Chr5:99035773-99035792 | None:intergenic | 35.0% | |
| TTGGAAGGAATATTTGTGTC+TGG | - | Chr5:99035754-99035773 | None:intergenic | 35.0% | |
| !! | ATGGCTTTTTGAAGACTCTT+CGG | - | Chr5:99035678-99035697 | None:intergenic | 35.0% |
| TCATCGTCACTGTCTTCCTT+AGG | - | Chr5:99035787-99035806 | None:intergenic | 45.0% | |
| ATCCGAAGATTCTCCGTCTC+CGG | + | Chr5:99035696-99035715 | MsG0580029592.01.T01:CDS | 50.0% | |
| CGTCACTGTCTTCCTTAGGA+TGG | - | Chr5:99035783-99035802 | None:intergenic | 50.0% | |
| GACTCATCGTCACCGGAAAT+TGG | - | Chr5:99035727-99035746 | None:intergenic | 50.0% | |
| ! | TCTCCGGCTAGTCCAATTTC+CGG | + | Chr5:99035712-99035731 | MsG0580029592.01.T01:CDS | 50.0% |
| !! | GAAATTGGACTAGCCGGAGA+CGG | - | Chr5:99035712-99035731 | None:intergenic | 50.0% |
| !! | TCACCGGAAATTGGACTAGC+CGG | - | Chr5:99035718-99035737 | None:intergenic | 50.0% |
| AGCCGGAGACGGAGAATCTT+CGG | - | Chr5:99035701-99035720 | None:intergenic | 55.0% | |
| GGAGACGGAGAATCTTCGGA+TGG | - | Chr5:99035697-99035716 | None:intergenic | 55.0% | |
| TGGCGCAGACTCATCGTCAC+CGG | - | Chr5:99035734-99035753 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 99035661 | 99035930 | 99035661 | ID=MsG0580029592.01;Name=MsG0580029592.01 |
| Chr5 | mRNA | 99035661 | 99035930 | 99035661 | ID=MsG0580029592.01.T01;Parent=MsG0580029592.01;Name=MsG0580029592.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|89 |
| Chr5 | exon | 99035661 | 99035930 | 99035661 | ID=MsG0580029592.01.T01:exon:26038;Parent=MsG0580029592.01.T01 |
| Chr5 | CDS | 99035661 | 99035930 | 99035661 | ID=MsG0580029592.01.T01:cds;Parent=MsG0580029592.01.T01 |
| Gene Sequence |
| Protein sequence |
