AlfalfaGEDB | MsG0680033926.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033926.01

KEGG: -
GO: GO:0003824
PFAM: PF00266

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033926.01.T01	XP_013452539.2	96.078	510	17	1	1	510	122	628	0	979

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033926.01.T01	A0A396HH98	96.078	510	17	1	1	510	122	628	0.0	979

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048185.01	MsG0680033926.01	-0.805728	1.160351e-49	6.185224e-47
MsG0080048968.01	MsG0680033926.01	0.819789	9.655560e-53	7.503844e-50
MsG0180000611.01	MsG0680033926.01	0.835707	1.434560e-56	1.768551e-53
MsG0180000821.01	MsG0680033926.01	0.812404	4.311875e-51	2.739273e-48
MsG0180001311.01	MsG0680033926.01	0.838856	2.242377e-57	3.041997e-54
MsG0180001712.01	MsG0680033926.01	0.808071	3.708146e-50	2.099801e-47
MsG0180002100.01	MsG0680033926.01	0.810259	1.260048e-50	7.559605e-48
MsG0180002140.01	MsG0680033926.01	0.807024	6.184845e-50	3.408003e-47
MsG0180002992.01	MsG0680033926.01	0.841661	4.149585e-58	6.139474e-55
MsG0180003379.01	MsG0680033926.01	0.817025	4.084084e-52	2.940901e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033926.01.T01	MTR_6g464840	95.882	510	18	1	1	510	122	628	0.0	975
MsG0680033926.01.T01	MTR_6g089580	95.686	510	19	1	1	510	122	628	0.0	975
MsG0680033926.01.T01	MTR_1g101790	68.401	538	127	10	2	510	120	643	0.0	713
MsG0680033926.01.T01	MTR_1g101790	67.932	527	126	10	2	499	120	632	0.0	691
MsG0680033926.01.T01	MTR_4g102620	50.501	499	203	11	30	510	143	615	1.48e-165	482
MsG0680033926.01.T01	MTR_8g079040	51.528	229	105	3	31	253	172	400	2.80e-70	241
MsG0680033926.01.T01	MTR_8g079040	45.614	171	79	4	337	493	751	921	3.59e-36	143
MsG0680033926.01.T01	MTR_8g079020	51.092	229	106	3	31	253	172	400	1.51e-69	239
MsG0680033926.01.T01	MTR_8g079020	43.860	171	82	3	337	493	755	925	3.80e-34	137
MsG0680033926.01.T01	MTR_3g099200	29.146	199	122	7	54	245	134	320	8.88e-17	81.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033926.01.T01	AT5G51920	51.883	478	168	6	25	499	138	556	3.94e-166	482
MsG0680033926.01.T01	AT4G22980	42.710	487	214	10	30	505	125	557	8.67e-123	371
MsG0680033926.01.T01	AT5G66950	50.847	236	110	3	24	253	164	399	1.00e-70	242
MsG0680033926.01.T01	AT5G66950	45.143	175	91	2	326	495	681	855	2.39e-38	150
MsG0680033926.01.T01	AT4G37100	41.613	310	169	4	24	327	170	473	2.31e-69	239
MsG0680033926.01.T01	AT4G37100	43.333	180	92	4	323	492	696	875	1.51e-35	142
MsG0680033926.01.T01	AT2G23520	48.908	229	111	3	31	253	173	401	9.64e-68	234
MsG0680033926.01.T01	AT2G23520	37.349	249	116	8	263	492	649	876	1.53e-33	135

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Find 67 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTTGATATTGAATGT+AGG	0.544123	6:+76606046	MsG0680033926.01.T01:CDS
AAAAGGCAACCAAATCCAGA+AGG	0.598988	6:-76605712	None:intergenic
AAAGGCAACCAAATCCAGAA+GGG	0.648687	6:-76605711	None:intergenic
AACGGCATATCGGAAAATTG+AGG	0.597999	6:-76605112	None:intergenic
AAGACTTTGCTTCTTCATGG+TGG	0.585075	6:+76605166	MsG0680033926.01.T01:CDS
AAGGTAAGTATTGAAACTTG+CGG	0.699855	6:-76605811	None:intergenic
AATATATTGAATGCAATTGC+AGG	0.415602	6:-76606231	None:intergenic
AATTTGTGAAATTGAGGAGG+TGG	0.569049	6:+76605972	MsG0680033926.01.T01:CDS
ACTGTTGTTACTGCTGCATT+AGG	0.320994	6:+76606429	MsG0680033926.01.T01:CDS
AGAAAACTGTTGAAGTTTAA+CGG	0.366475	6:+76606559	MsG0680033926.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAAGTATAGCGAAAGAGAA+TGG	+	Chr6:76605571-76605590	MsG0680033926.01.T01:CDS	30.0%
CAAGTTCAAATCGATGAAAA+GGG	+	Chr6:76606000-76606019	MsG0680033926.01.T01:CDS	30.0%
GAATTCAGTCAACAAAATTG+AGG	+	Chr6:76605449-76605468	MsG0680033926.01.T01:CDS	30.0%
GAGCAAGATATCCTTATATT+TGG	+	Chr6:76605548-76605567	MsG0680033926.01.T01:CDS	30.0%
GTATCTCTAGTGAAGATTTA+TGG	+	Chr6:76606183-76606202	MsG0680033926.01.T01:CDS	30.0%
TGAAATTTGTGAAATTGAGG+AGG	+	Chr6:76605969-76605988	MsG0680033926.01.T01:CDS	30.0%
TTGGAAACAAATTCTTCTGT+AGG	+	Chr6:76605760-76605779	MsG0680033926.01.T01:CDS	30.0%
TTTAGTATATCCTGCAAAAC+AGG	+	Chr6:76605136-76605155	MsG0680033926.01.T01:CDS	30.0%
AATTTGTGAAATTGAGGAGG+TGG	+	Chr6:76605972-76605991	MsG0680033926.01.T01:CDS	35.0%
AGATAAGTATGCCGATGAAA+AGG	+	Chr6:76606356-76606375	MsG0680033926.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	76605046	76606578	76605046	ID=MsG0680033926.01;Name=MsG0680033926.01
Chr6	mRNA	76605046	76606578	76605046	ID=MsG0680033926.01.T01;Parent=MsG0680033926.01;Name=MsG0680033926.01.T01;_AED=0.16;_eAED=0.16;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|510
Chr6	exon	76605046	76606578	76605046	ID=MsG0680033926.01.T01:exon:34303;Parent=MsG0680033926.01.T01
Chr6	CDS	76605046	76606578	76605046	ID=MsG0680033926.01.T01:cds;Parent=MsG0680033926.01.T01

Gene Sequence

>MsG0680033926.01.T01

ATGCAACAATATCATGATACATCGAAAACAAACATTTCGAGTCATGAAAAAACACAAGAATCTCTACCTCAATTTTCCGATATGCCGTTTTTTAGTATATCCTGCAAAACAGGAAATTTGAAGACTTTGCTTCTTCATGGTGGAAAAGATTCAGAATTCGAGTCAGCAATGAGAAAAAGAATCATGAATTACCTTAACATATCGGAAAACGATTACTTCATGGTTTTCACAGCGAATAGAACATCGGCTTTCAAACTTGTAGCAGATTGTTACAATTTCCAAAAGAGTAGAAAGCTTTTAACAGTTTATGACCATGAAAGCGAAGCGGTGGAAGCGATGATTAGTTCTTCGGAAAAACGAGGCGCAAAAGCTATGTCAGCAGAGTTTTCATGGCCAAGGTTGAGAATTCAGTCAACAAAATTGAGGAAAATGATTGTAAGTAAGAGAAAGAAGAAGAAAAATAAGGGTCTTTTTGTTTTGCCTCTTCATTCAAGAGTAACAGGAGCAAGATATCCTTATATTTGGATAAGTATAGCGAAAGAGAATGGTTGGAATGTTTTGGTTGATGCATGTGCATTAGGACCAAAAGACATGGATTGTTTTGGTCTTTCGCTTTTTCAACCAGATTTTCTTATTTGTTCTTGTTATAAAGTTTTCGGAGAAAACCCTTCTGGATTTGGTTGCCTTTTTATCAAGAAAAGTGCTACGTCGATTTTGGAAACAAATTCTTCTGTAGGAATAGTGAACCTTGTGCAAGAAAAAAAGCCGCAAGTTTCAATACTTACCTTACATTTAGATGAAGAAGAACCATTTTATATGACATCTTTGTCTAACATGAAACAAACATCGCAACCTGCAAATTTCGTAGAAGGAGAATCGTCTGAGATGAAAATTGTAGAGGCACCAAGTGTGTCAAAACCATCTGAAATTTGTGAAATTGAGGAGGTGGAAAAGCAAGTTCAAATCGATGAAAAGGGTGTTAAAGAAAGTGATAAAAATGAAAACTTTGATATTGAATGTAGGTGCTTAGATCAAGTGGATTCATTAGGATTGTTACTGATCAATGATCGTGCGAGGTACTTGATTAACTGGTTGGTGAATTCGATGTTGAAGCTTAAGCATCCTAACTCCGAAGGGGTATCTCTAGTGAAGATTTATGGTCCAAAAGTTAAATTTGATAGAGGACCTGCAATTGCATTCAATATATTTGATTGGAAAGGCGAAAAAGTTGAACCTGTTCTCGTGCAAAAACTCGCTGATAGAAGCAATATTTCTATTAGCTATGGATTGTTGCATCATATATGGTTTGCAGATAAGTATGCCGATGAAAAGGGAAGAGTACTAAAAGACGAGAAAAAGAAAGATAAAGAAAAAGTTGGAATAACTGTTGTTACTGCTGCATTAGGTTTTTTGGCTAATTTTGAAGATATCTATAATCTTTGGTGTTTTGTTGCGAGGTTTCTTGATGCTGATTTTGTCGAGAAGGAGAGGTGGAGATATACTGCTCTCAATCAGAAAACTGTTGAAGTTTAA

Protein sequence

>MsG0680033926.01.T01

MQQYHDTSKTNISSHEKTQESLPQFSDMPFFSISCKTGNLKTLLLHGGKDSEFESAMRKRIMNYLNISENDYFMVFTANRTSAFKLVADCYNFQKSRKLLTVYDHESEAVEAMISSSEKRGAKAMSAEFSWPRLRIQSTKLRKMIVSKRKKKKNKGLFVLPLHSRVTGARYPYIWISIAKENGWNVLVDACALGPKDMDCFGLSLFQPDFLICSCYKVFGENPSGFGCLFIKKSATSILETNSSVGIVNLVQEKKPQVSILTLHLDEEEPFYMTSLSNMKQTSQPANFVEGESSEMKIVEAPSVSKPSEICEIEEVEKQVQIDEKGVKESDKNENFDIECRCLDQVDSLGLLLINDRARYLINWLVNSMLKLKHPNSEGVSLVKIYGPKVKFDRGPAIAFNIFDWKGEKVEPVLVQKLADRSNISISYGLLHHIWFADKYADEKGRVLKDEKKKDKEKVGITVVTAALGFLANFEDIYNLWCFVARFLDADFVEKERWRYTALNQKTVEV*