Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007716.01.T01 | XP_003594548.2 | 95.146 | 412 | 19 | 1 | 1 | 411 | 1 | 412 | 0 | 816 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007716.01.T01 | G7IKW8 | 95.146 | 412 | 19 | 1 | 1 | 411 | 1 | 412 | 0.0 | 816 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000399.01 | MsG0280007716.01 | 0.852294 | 5.074174e-61 | 1.055480e-57 |
| MsG0180001513.01 | MsG0280007716.01 | 0.810345 | 1.207093e-50 | 7.258202e-48 |
| MsG0180001515.01 | MsG0280007716.01 | 0.810434 | 1.155097e-50 | 6.961716e-48 |
| MsG0180002808.01 | MsG0280007716.01 | 0.809186 | 2.142858e-50 | 1.249758e-47 |
| MsG0180002935.01 | MsG0280007716.01 | 0.831823 | 1.341973e-55 | 1.473184e-52 |
| MsG0180003372.01 | MsG0280007716.01 | 0.806962 | 6.375106e-50 | 3.507165e-47 |
| MsG0180003375.01 | MsG0280007716.01 | 0.816487 | 5.393437e-52 | 3.826593e-49 |
| MsG0180004424.01 | MsG0280007716.01 | 0.800090 | 1.696225e-48 | 7.828702e-46 |
| MsG0180005762.01 | MsG0280007716.01 | 0.803848 | 2.865527e-49 | 1.455313e-46 |
| MsG0280006898.01 | MsG0280007716.01 | 0.810459 | 1.140958e-50 | 6.881097e-48 |
| MsG0280007216.01 | MsG0280007716.01 | 0.803720 | 3.046567e-49 | 1.542080e-46 |
| MsG0280007716.01 | MsG0280008226.01 | 0.815650 | 8.292774e-52 | 5.751078e-49 |
| MsG0280007716.01 | MsG0280008659.01 | 0.813637 | 2.314070e-51 | 1.519361e-48 |
| MsG0280007716.01 | MsG0280010410.01 | 0.818195 | 2.224776e-52 | 1.654193e-49 |
| MsG0280007716.01 | MsG0280010802.01 | 0.810976 | 8.816973e-51 | 5.392280e-48 |
| MsG0280007716.01 | MsG0280011267.01 | 0.807363 | 5.242517e-50 | 2.914268e-47 |
| MsG0280007716.01 | MsG0380011635.01 | 0.829247 | 5.727428e-55 | 5.829727e-52 |
| MsG0280007716.01 | MsG0380011974.01 | 0.813320 | 2.716970e-51 | 1.768634e-48 |
| MsG0280007716.01 | MsG0380013272.01 | 0.800049 | 1.729692e-48 | 7.974618e-46 |
| MsG0280007716.01 | MsG0380013285.01 | 0.811645 | 6.311527e-51 | 3.928014e-48 |
| MsG0280007716.01 | MsG0380015080.01 | 0.821562 | 3.779248e-53 | 3.085389e-50 |
| MsG0280007716.01 | MsG0380015110.01 | 0.805623 | 1.221065e-49 | 6.490813e-47 |
| MsG0280007716.01 | MsG0380015130.01 | 0.807742 | 4.355991e-50 | 2.445579e-47 |
| MsG0280007716.01 | MsG0480018440.01 | 0.803441 | 3.480291e-49 | 1.749070e-46 |
| MsG0280007716.01 | MsG0480018522.01 | 0.800445 | 1.436595e-48 | 6.690330e-46 |
| MsG0280007716.01 | MsG0480019923.01 | 0.834115 | 3.611176e-56 | 4.244208e-53 |
| MsG0280007716.01 | MsG0480020814.01 | 0.807849 | 4.133313e-50 | 2.326909e-47 |
| MsG0280007716.01 | MsG0480020958.01 | 0.805018 | 1.634553e-49 | 8.554738e-47 |
| MsG0280007716.01 | MsG0480021133.01 | 0.817372 | 3.412705e-52 | 2.480863e-49 |
| MsG0280007716.01 | MsG0480022309.01 | 0.831992 | 1.218930e-55 | 1.344698e-52 |
| MsG0280007716.01 | MsG0480022833.01 | 0.815488 | 9.009197e-52 | 6.220413e-49 |
| MsG0280007716.01 | MsG0580024086.01 | 0.860310 | 2.258240e-63 | 6.145576e-60 |
| MsG0280007716.01 | MsG0580024202.01 | 0.827461 | 1.544532e-54 | 1.492185e-51 |
| MsG0280007716.01 | MsG0580024207.01 | 0.809346 | 1.980326e-50 | 1.159892e-47 |
| MsG0280007716.01 | MsG0580024217.01 | 0.810684 | 1.019682e-50 | 6.187157e-48 |
| MsG0280007716.01 | MsG0580024219.01 | 0.801167 | 1.023155e-48 | 4.852976e-46 |
| MsG0280007716.01 | MsG0580024408.01 | 0.803646 | 3.156384e-49 | 1.594643e-46 |
| MsG0280007716.01 | MsG0580024990.01 | 0.800038 | 1.738286e-48 | 8.012082e-46 |
| MsG0280007716.01 | MsG0580025023.01 | 0.804228 | 2.389231e-49 | 1.225215e-46 |
| MsG0280007716.01 | MsG0580025965.01 | 0.810298 | 1.235688e-50 | 7.420764e-48 |
| MsG0280007716.01 | MsG0580026492.01 | 0.822006 | 2.984311e-53 | 2.467166e-50 |
| MsG0280007716.01 | MsG0580030145.01 | 0.800787 | 1.223268e-48 | 5.746316e-46 |
| MsG0280007716.01 | MsG0680031009.01 | 0.822806 | 1.945456e-53 | 1.645452e-50 |
| MsG0280007716.01 | MsG0680032343.01 | 0.812047 | 5.160426e-51 | 3.246358e-48 |
| MsG0280007716.01 | MsG0680033347.01 | 0.829175 | 5.962472e-55 | 6.055921e-52 |
| MsG0280007716.01 | MsG0680035841.01 | 0.832903 | 7.246483e-56 | 8.212623e-53 |
| MsG0280007716.01 | MsG0780036208.01 | 0.811840 | 5.724094e-51 | 3.581081e-48 |
| MsG0280007716.01 | MsG0780036370.01 | 0.810692 | 1.015934e-50 | 6.165656e-48 |
| MsG0280007716.01 | MsG0780038519.01 | 0.811567 | 6.564047e-51 | 4.077023e-48 |
| MsG0280007716.01 | MsG0780039923.01 | 0.807418 | 5.105445e-50 | 2.842159e-47 |
| MsG0280007716.01 | MsG0780040615.01 | 0.800587 | 1.344170e-48 | 6.282494e-46 |
| MsG0280007716.01 | MsG0780040977.01 | 0.830627 | 2.640539e-55 | 2.797335e-52 |
| MsG0280007716.01 | MsG0780040991.01 | 0.826374 | 2.809388e-54 | 2.630052e-51 |
| MsG0280007716.01 | MsG0880041978.01 | 0.819662 | 1.032717e-52 | 7.996745e-50 |
| MsG0280007716.01 | MsG0880043017.01 | 0.818507 | 1.890994e-52 | 1.418167e-49 |
| MsG0280007716.01 | MsG0880043321.01 | 0.802982 | 4.332958e-49 | 2.151995e-46 |
| MsG0280007716.01 | MsG0880043364.01 | 0.804673 | 1.929682e-49 | 1.000998e-46 |
| MsG0280007716.01 | MsG0880043481.01 | 0.824322 | 8.596004e-54 | 7.588119e-51 |
| MsG0280007716.01 | MsG0880046402.01 | 0.802270 | 6.074729e-49 | 2.963024e-46 |
| MsG0280007716.01 | MsG0880046403.01 | 0.808973 | 2.380662e-50 | 1.380456e-47 |
| MsG0280007716.01 | MsG0880046429.01 | 0.804679 | 1.924145e-49 | 9.982902e-47 |
| MsG0280007716.01 | MsG0880047398.01 | 0.805649 | 1.205595e-49 | 6.412924e-47 |
| MsG0280007716.01 | MsG0880047406.01 | -0.807364 | 5.241726e-50 | 2.913867e-47 |
| MsG0280007716.01 | MsG0880047542.01 | 0.815855 | 7.464000e-52 | 5.204992e-49 |
| MsG0280007716.01 | MsG0880047714.01 | 0.819089 | 1.394867e-52 | 1.063040e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007716.01.T01 | MTR_2g030460 | 95.146 | 412 | 19 | 1 | 1 | 411 | 1 | 412 | 0.0 | 816 |
| MsG0280007716.01.T01 | MTR_2g030460 | 96.108 | 334 | 12 | 1 | 1 | 333 | 1 | 334 | 0.0 | 666 |
| MsG0280007716.01.T01 | MTR_1g052640 | 68.039 | 413 | 129 | 3 | 1 | 411 | 1 | 412 | 0.0 | 603 |
| MsG0280007716.01.T01 | MTR_2g030470 | 67.640 | 411 | 131 | 2 | 2 | 411 | 5 | 414 | 0.0 | 596 |
| MsG0280007716.01.T01 | MTR_2g030470 | 66.422 | 408 | 135 | 2 | 1 | 407 | 416 | 822 | 0.0 | 579 |
| MsG0280007716.01.T01 | MTR_0083s0100 | 67.070 | 413 | 133 | 3 | 1 | 411 | 1 | 412 | 0.0 | 585 |
| MsG0280007716.01.T01 | MTR_4g094550 | 60.382 | 419 | 156 | 4 | 1 | 411 | 1 | 417 | 0.0 | 545 |
| MsG0280007716.01.T01 | MTR_2g030480 | 62.621 | 412 | 132 | 3 | 1 | 411 | 1 | 391 | 0.0 | 544 |
| MsG0280007716.01.T01 | MTR_6g048350 | 60.000 | 415 | 155 | 5 | 1 | 407 | 1 | 412 | 0.0 | 541 |
| MsG0280007716.01.T01 | MTR_4g094560 | 60.442 | 407 | 156 | 4 | 1 | 407 | 1 | 402 | 0.0 | 536 |
| MsG0280007716.01.T01 | MTR_4g094548 | 58.894 | 416 | 160 | 3 | 1 | 407 | 1 | 414 | 0.0 | 533 |
| MsG0280007716.01.T01 | MTR_4g094555 | 57.995 | 419 | 165 | 3 | 1 | 411 | 1 | 416 | 0.0 | 530 |
| MsG0280007716.01.T01 | MTR_4g094570 | 58.151 | 411 | 161 | 4 | 9 | 411 | 9 | 416 | 0.0 | 526 |
| MsG0280007716.01.T01 | MTR_4g094562 | 58.473 | 419 | 163 | 4 | 1 | 411 | 1 | 416 | 0.0 | 525 |
| MsG0280007716.01.T01 | MTR_6g048310 | 60.560 | 393 | 142 | 5 | 22 | 407 | 20 | 406 | 0.0 | 517 |
| MsG0280007716.01.T01 | MTR_1g052640 | 66.667 | 342 | 111 | 3 | 1 | 340 | 1 | 341 | 8.54e-174 | 489 |
| MsG0280007716.01.T01 | MTR_1g052640 | 66.667 | 288 | 93 | 3 | 1 | 286 | 1 | 287 | 2.36e-144 | 412 |
| MsG0280007716.01.T01 | MTR_8g039300 | 43.128 | 422 | 211 | 8 | 1 | 408 | 1 | 407 | 3.30e-128 | 376 |
| MsG0280007716.01.T01 | MTR_6g089930 | 43.333 | 420 | 209 | 8 | 1 | 406 | 1 | 405 | 4.99e-128 | 375 |
| MsG0280007716.01.T01 | MTR_6g090090 | 44.524 | 420 | 194 | 9 | 1 | 406 | 27 | 421 | 2.85e-127 | 374 |
| MsG0280007716.01.T01 | MTR_4g094565 | 46.229 | 411 | 145 | 6 | 1 | 411 | 1 | 335 | 4.87e-126 | 368 |
| MsG0280007716.01.T01 | MTR_3g035040 | 42.417 | 422 | 214 | 8 | 1 | 408 | 1 | 407 | 2.40e-121 | 358 |
| MsG0280007716.01.T01 | MTR_4g094562 | 54.545 | 297 | 124 | 4 | 1 | 289 | 1 | 294 | 3.40e-119 | 350 |
| MsG0280007716.01.T01 | MTR_1g035220 | 42.278 | 395 | 175 | 10 | 1 | 379 | 1 | 358 | 1.53e-105 | 316 |
| MsG0280007716.01.T01 | MTR_4g094565 | 51.748 | 286 | 115 | 5 | 1 | 286 | 1 | 263 | 5.69e-103 | 308 |
| MsG0280007716.01.T01 | MTR_1g062750 | 46.835 | 237 | 108 | 4 | 60 | 282 | 2 | 234 | 6.62e-72 | 226 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007716.01.T01 | AT5G61820 | 49.762 | 420 | 181 | 6 | 11 | 408 | 9 | 420 | 9.53e-146 | 423 |
| MsG0280007716.01.T01 | AT5G61820 | 49.762 | 420 | 181 | 6 | 11 | 408 | 9 | 420 | 9.53e-146 | 423 |
Find 99 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATTCCAGTTCCATATTT+TGG | 0.072146 | 2:-19544318 | None:intergenic |
| CCTAAACCTCCTTTGTAATT+TGG | 0.119485 | 2:-19543403 | None:intergenic |
| CCTTTGTAATTTGGACTTAA+AGG | 0.120999 | 2:-19543394 | None:intergenic |
| CCTGGACTTAATTCAATCTT+AGG | 0.232069 | 2:-19542914 | None:intergenic |
| AATGGGACATTTCTATTTCT+TGG | 0.240338 | 2:+19544473 | MsG0280007716.01.T01:CDS |
| GCTGAACTAGTAGATGAATA+TGG | 0.253004 | 2:+19543007 | MsG0280007716.01.T01:CDS |
| CCAAATTACAAAGGAGGTTT+AGG | 0.264358 | 2:+19543403 | MsG0280007716.01.T01:CDS |
| ATTATTATGGTGAGAAATAA+TGG | 0.269974 | 2:+19543124 | MsG0280007716.01.T01:CDS |
| AGATTAAATGTCACATATTA+TGG | 0.284918 | 2:-19544515 | None:intergenic |
| ACTACCATTCCCATGGCTTT+TGG | 0.289841 | 2:-19543747 | None:intergenic |
| AATGGTGAAGATAAGTTTCT+TGG | 0.294271 | 2:-19543045 | None:intergenic |
| CCCACATAATTGTGTACTTT+AGG | 0.295452 | 2:-19543492 | None:intergenic |
| TGCAACTCAATCCCAAAATA+TGG | 0.295691 | 2:+19544306 | MsG0280007716.01.T01:CDS |
| ACATATATTCCTGACCCTTA+TGG | 0.320031 | 2:+19543211 | MsG0280007716.01.T01:CDS |
| CTCAAGATTGGATGCTTTCA+TGG | 0.320835 | 2:+19542798 | MsG0280007716.01.T01:CDS |
| GACCCTTATGGAGTAGAAAT+TGG | 0.321397 | 2:+19543223 | MsG0280007716.01.T01:CDS |
| ATCTTGATAGAACCTGGTTT+AGG | 0.327289 | 2:-19544387 | None:intergenic |
| TTATGCCAAGATGATATTCT+TGG | 0.334835 | 2:+19543148 | MsG0280007716.01.T01:CDS |
| TCTTGATAGAACCTGGTTTA+GGG | 0.341330 | 2:-19544386 | None:intergenic |
| AATCTACATTGACCATTATC+AGG | 0.346043 | 2:-19543433 | None:intergenic |
| CATTTATTGTAACGTTATAT+AGG | 0.358741 | 2:-19543357 | None:intergenic |
| ACACAATTATGTGGGTTGAT+TGG | 0.359043 | 2:+19543500 | MsG0280007716.01.T01:CDS |
| TCAAGTAAGTAAATCTTAAC+TGG | 0.369070 | 2:-19543538 | None:intergenic |
| GCGATTAGATCAACCCTATA+TGG | 0.370275 | 2:+19543854 | MsG0280007716.01.T01:CDS |
| TTAGGATGTTGTCCTGATAA+TGG | 0.372817 | 2:+19543421 | MsG0280007716.01.T01:CDS |
| TAACCAAACAGATATTATTA+TGG | 0.372909 | 2:+19543111 | MsG0280007716.01.T01:CDS |
| GATATTCTTGGACAATACTA+TGG | 0.376133 | 2:+19543160 | MsG0280007716.01.T01:CDS |
| CACAATTATGTGGGTTGATT+GGG | 0.382907 | 2:+19543501 | MsG0280007716.01.T01:CDS |
| TTACCAATTTCTACTCCATA+AGG | 0.383266 | 2:-19543226 | None:intergenic |
| AATTTGTTTGAAATTGATCC+TGG | 0.396040 | 2:-19542932 | None:intergenic |
| TCACCATAATAATATCTGTT+TGG | 0.399279 | 2:-19543114 | None:intergenic |
| CATAATGCACACCGGTGTAA+TGG | 0.410797 | 2:+19544455 | MsG0280007716.01.T01:CDS |
| GGTTCTATCAAGATATTTGA+TGG | 0.412273 | 2:+19544396 | MsG0280007716.01.T01:CDS |
| ACCTAAAGTACACAATTATG+TGG | 0.432101 | 2:+19543491 | MsG0280007716.01.T01:CDS |
| AATGAGAAAGGGTATCTTGT+AGG | 0.436332 | 2:+19544348 | MsG0280007716.01.T01:CDS |
| ATGACATACCTGTCCATATA+GGG | 0.438172 | 2:-19543867 | None:intergenic |
| ATAATGCACACCGGTGTAAT+GGG | 0.442600 | 2:+19544456 | MsG0280007716.01.T01:CDS |
| TTGAGTGCAATATGACCCTT+TGG | 0.444188 | 2:-19542974 | None:intergenic |
| AAGGGGTGTGGAAGATAGAA+TGG | 0.449147 | 2:+19543312 | MsG0280007716.01.T01:CDS |
| ATGTTTGATGCTCATTCCAT+TGG | 0.452261 | 2:-19543593 | None:intergenic |
| TAGGTAAAGGACATGTTTAG+TGG | 0.454745 | 2:-19543473 | None:intergenic |
| TCAATCCCAAAATATGGAAC+TGG | 0.455772 | 2:+19544312 | MsG0280007716.01.T01:CDS |
| CATGACATACCTGTCCATAT+AGG | 0.458282 | 2:-19543868 | None:intergenic |
| ATCCTTCACAAATTCCTAAA+GGG | 0.463218 | 2:+19543248 | MsG0280007716.01.T01:CDS |
| AAGAAACTTATCTTCACCAT+TGG | 0.467809 | 2:+19543047 | MsG0280007716.01.T01:CDS |
| CTGAACTAGTAGATGAATAT+GGG | 0.470249 | 2:+19543008 | MsG0280007716.01.T01:CDS |
| AATCCTTCACAAATTCCTAA+AGG | 0.481001 | 2:+19543247 | MsG0280007716.01.T01:CDS |
| TACCAATTTCTACTCCATAA+GGG | 0.483211 | 2:-19543225 | None:intergenic |
| TGTTTGATGCTCATTCCATT+GGG | 0.484253 | 2:-19543592 | None:intergenic |
| AGGGGTGTGGAAGATAGAAT+GGG | 0.489368 | 2:+19543313 | MsG0280007716.01.T01:CDS |
| CTTGGACAATACTATGGCCT+AGG | 0.493115 | 2:+19543166 | MsG0280007716.01.T01:CDS |
| TGCCTCTAGTTTCAGCTCCT+AGG | 0.494591 | 2:-19543183 | None:intergenic |
| GTTACAATAAATGCATTAAG+TGG | 0.500168 | 2:+19543367 | MsG0280007716.01.T01:CDS |
| GGCATTAATGTTAGGATGGG+AGG | 0.502702 | 2:+19544277 | MsG0280007716.01.T01:intron |
| CCTAAGATTGAATTAAGTCC+AGG | 0.504942 | 2:+19542914 | MsG0280007716.01.T01:CDS |
| ATTTCTACTCCATAAGGGTC+AGG | 0.506350 | 2:-19543220 | None:intergenic |
| ATTGTCCAAGAATATCATCT+TGG | 0.520826 | 2:-19543153 | None:intergenic |
| ATGTCCATGCAATTGATACA+AGG | 0.523091 | 2:+19543293 | MsG0280007716.01.T01:CDS |
| TAATTGTGTACTTTAGGTAA+AGG | 0.526319 | 2:-19543486 | None:intergenic |
| TATGATGTTGAATTTCCAAA+GGG | 0.528720 | 2:+19542959 | MsG0280007716.01.T01:CDS |
| TCAAGATTGGATGCTTTCAT+GGG | 0.528759 | 2:+19542799 | MsG0280007716.01.T01:CDS |
| ATGGAAGCAGGAAATGAGAA+AGG | 0.530156 | 2:+19544336 | MsG0280007716.01.T01:CDS |
| AAAAGCCATGGGAATGGTAG+TGG | 0.532477 | 2:+19543749 | MsG0280007716.01.T01:CDS |
| TGCAATTGATACAAGGGGTG+TGG | 0.533630 | 2:+19543300 | MsG0280007716.01.T01:CDS |
| CCCAAAATATGGAACTGGAA+TGG | 0.534405 | 2:+19544317 | MsG0280007716.01.T01:CDS |
| ATGGGCAATGGTGCTAGTGT+TGG | 0.536000 | 2:+19542817 | MsG0280007716.01.T01:CDS |
| TATGGAACTGGAATGGAAGC+AGG | 0.536281 | 2:+19544324 | MsG0280007716.01.T01:CDS |
| CACCCCTTGTATCAATTGCA+TGG | 0.547570 | 2:-19543297 | None:intergenic |
| GGGACATTTCTATTTCTTGG+TGG | 0.548487 | 2:+19544476 | MsG0280007716.01.T01:CDS |
| TAGATCAACCCTATATGGAC+AGG | 0.548766 | 2:+19543859 | MsG0280007716.01.T01:CDS |
| TCAAATATCTTGATAGAACC+TGG | 0.548862 | 2:-19544393 | None:intergenic |
| GGTTATATAATCTACAGTGC+TGG | 0.556126 | 2:+19543815 | MsG0280007716.01.T01:CDS |
| TTATGATGTTGAATTTCCAA+AGG | 0.557376 | 2:+19542958 | MsG0280007716.01.T01:CDS |
| AAACCTTGCACCAAAAGCCA+TGG | 0.562734 | 2:+19543737 | MsG0280007716.01.T01:CDS |
| AAAATATCATATAATCCCAA+TGG | 0.565537 | 2:+19543577 | MsG0280007716.01.T01:CDS |
| ATAATGGTCAATGTAGATTG+AGG | 0.569226 | 2:+19543437 | MsG0280007716.01.T01:CDS |
| TTAAGTCCAAATTACAAAGG+AGG | 0.571780 | 2:+19543397 | MsG0280007716.01.T01:CDS |
| GCATTAATGTTAGGATGGGA+GGG | 0.573100 | 2:+19544278 | MsG0280007716.01.T01:intron |
| TGCACCAAAAGCCATGGGAA+TGG | 0.582420 | 2:+19543743 | MsG0280007716.01.T01:CDS |
| CCTAAAGTACACAATTATGT+GGG | 0.583692 | 2:+19543492 | MsG0280007716.01.T01:CDS |
| TACCCTTTAGGAATTTGTGA+AGG | 0.593512 | 2:-19543250 | None:intergenic |
| TGGAAGCAGGAAATGAGAAA+GGG | 0.595316 | 2:+19544337 | MsG0280007716.01.T01:CDS |
| AACCTTGCACCAAAAGCCAT+GGG | 0.602557 | 2:+19543738 | MsG0280007716.01.T01:CDS |
| GAGCATCAAACATAATTGCA+AGG | 0.603328 | 2:+19543603 | MsG0280007716.01.T01:CDS |
| TTGGATGCTTTCATGGGCAA+TGG | 0.606467 | 2:+19542805 | MsG0280007716.01.T01:CDS |
| TGAAGATAAGTTTCTTGGAG+AGG | 0.609861 | 2:-19543040 | None:intergenic |
| TGTCCATGCAATTGATACAA+GGG | 0.611124 | 2:+19543294 | MsG0280007716.01.T01:CDS |
| CTGTTGGTAGCTAATAAGCA+TGG | 0.615939 | 2:-19542851 | None:intergenic |
| GGTCAATGTAGATTGAGGAA+AGG | 0.621157 | 2:+19543442 | MsG0280007716.01.T01:CDS |
| CACAACCACTACCATTCCCA+TGG | 0.624715 | 2:-19543754 | None:intergenic |
| GGCCTAGGAGCTGAAACTAG+AGG | 0.631350 | 2:+19543181 | MsG0280007716.01.T01:CDS |
| AGTGCTGGTCATCAACATGT+AGG | 0.634573 | 2:+19543830 | MsG0280007716.01.T01:CDS |
| GTCCATGCAATTGATACAAG+GGG | 0.639292 | 2:+19543295 | MsG0280007716.01.T01:CDS |
| CCTTTAAGTCCAAATTACAA+AGG | 0.642182 | 2:+19543394 | MsG0280007716.01.T01:CDS |
| AAATGTCACATATTATGGCG+TGG | 0.659304 | 2:-19544510 | None:intergenic |
| TCAACTTGCTACCCTAAACC+AGG | 0.667040 | 2:+19544375 | MsG0280007716.01.T01:CDS |
| ATAGAAATGTCCCATTACAC+CGG | 0.668859 | 2:-19544466 | None:intergenic |
| GGGGTGTGGAAGATAGAATG+GGG | 0.677538 | 2:+19543314 | MsG0280007716.01.T01:CDS |
| AACAACAGCATAATGCACAC+CGG | 0.718055 | 2:+19544447 | MsG0280007716.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGTTTTAACTTTTAAAAAT+CGG | - | Chr2:19544250-19544269 | None:intergenic | 10.0% |
| !!! | ATTTTTAAAAGTTAAAACTT+TGG | + | Chr2:19544250-19544269 | MsG0280007716.01.T01:intron | 10.0% |
| !! | TATATGTGAAAAACATTATT+TGG | + | Chr2:19543646-19543665 | MsG0280007716.01.T01:intron | 15.0% |
| !!! | GGTTTAATTTTTTAAAACAT+TGG | - | Chr2:19544027-19544046 | None:intergenic | 15.0% |
| !! | AAAATATCATATAATCCCAA+TGG | + | Chr2:19543577-19543596 | MsG0280007716.01.T01:CDS | 20.0% |
| !! | AAAGTAGAAAGGAATTAAAA+AGG | - | Chr2:19544196-19544215 | None:intergenic | 20.0% |
| !! | ATTATTATGGTGAGAAATAA+TGG | + | Chr2:19543124-19543143 | MsG0280007716.01.T01:CDS | 20.0% |
| !! | CATTTATTGTAACGTTATAT+AGG | - | Chr2:19543360-19543379 | None:intergenic | 20.0% |
| !! | CTCAATTAAATATGCTAATT+TGG | - | Chr2:19544048-19544067 | None:intergenic | 20.0% |
| !! | GTCAATATTAAAAAGTAGAA+AGG | - | Chr2:19544207-19544226 | None:intergenic | 20.0% |
| !! | TAACCAAACAGATATTATTA+TGG | + | Chr2:19543111-19543130 | MsG0280007716.01.T01:CDS | 20.0% |
| !!! | TTATTGTTTGTCACATTTTT+AGG | - | Chr2:19543094-19543113 | None:intergenic | 20.0% |
| ! | AAAAGTTAAAACTTTGGTTG+TGG | + | Chr2:19544256-19544275 | MsG0280007716.01.T01:intron | 25.0% |
| ! | AACAAATAAGATCAAACCAA+TGG | - | Chr2:19544086-19544105 | None:intergenic | 25.0% |
| ! | CAAAAACAATGTTAAATCAG+CGG | + | Chr2:19542880-19542899 | MsG0280007716.01.T01:CDS | 25.0% |
| ! | GTTACAATAAATGCATTAAG+TGG | + | Chr2:19543367-19543386 | MsG0280007716.01.T01:CDS | 25.0% |
| ! | TAATTGTGTACTTTAGGTAA+AGG | - | Chr2:19543489-19543508 | None:intergenic | 25.0% |
| ! | TATGATGTTGAATTTCCAAA+GGG | + | Chr2:19542959-19542978 | MsG0280007716.01.T01:CDS | 25.0% |
| ! | TCAAGTAAGTAAATCTTAAC+TGG | - | Chr2:19543541-19543560 | None:intergenic | 25.0% |
| ! | TCACCATAATAATATCTGTT+TGG | - | Chr2:19543117-19543136 | None:intergenic | 25.0% |
| ! | TTATGATGTTGAATTTCCAA+AGG | + | Chr2:19542958-19542977 | MsG0280007716.01.T01:CDS | 25.0% |
| !! | AATTTGTTTGAAATTGATCC+TGG | - | Chr2:19542935-19542954 | None:intergenic | 25.0% |
| !! | ATATAACCACCAGTTTTTAT+TGG | - | Chr2:19543803-19543822 | None:intergenic | 25.0% |
| !! | TATAACCACCAGTTTTTATT+GGG | - | Chr2:19543802-19543821 | None:intergenic | 25.0% |
| !!! | ATTGTTTTTGGTATTTCTGT+TGG | - | Chr2:19542870-19542889 | None:intergenic | 25.0% |
| AAGAAACTTATCTTCACCAT+TGG | + | Chr2:19543047-19543066 | MsG0280007716.01.T01:CDS | 30.0% | |
| AATCCTTCACAAATTCCTAA+AGG | + | Chr2:19543247-19543266 | MsG0280007716.01.T01:CDS | 30.0% | |
| AATCTACATTGACCATTATC+AGG | - | Chr2:19543436-19543455 | None:intergenic | 30.0% | |
| AATGGTGAAGATAAGTTTCT+TGG | - | Chr2:19543048-19543067 | None:intergenic | 30.0% | |
| ACCTAAAGTACACAATTATG+TGG | + | Chr2:19543491-19543510 | MsG0280007716.01.T01:CDS | 30.0% | |
| ATAATGGTCAATGTAGATTG+AGG | + | Chr2:19543437-19543456 | MsG0280007716.01.T01:CDS | 30.0% | |
| ATCCTTCACAAATTCCTAAA+GGG | + | Chr2:19543248-19543267 | MsG0280007716.01.T01:CDS | 30.0% | |
| ATTGTCCAAGAATATCATCT+TGG | - | Chr2:19543156-19543175 | None:intergenic | 30.0% | |
| CCTAAAGTACACAATTATGT+GGG | + | Chr2:19543492-19543511 | MsG0280007716.01.T01:CDS | 30.0% | |
| CCTTTAAGTCCAAATTACAA+AGG | + | Chr2:19543394-19543413 | MsG0280007716.01.T01:CDS | 30.0% | |
| CCTTTGTAATTTGGACTTAA+AGG | - | Chr2:19543397-19543416 | None:intergenic | 30.0% | |
| CTGAACTAGTAGATGAATAT+GGG | + | Chr2:19543008-19543027 | MsG0280007716.01.T01:CDS | 30.0% | |
| GATATTCTTGGACAATACTA+TGG | + | Chr2:19543160-19543179 | MsG0280007716.01.T01:CDS | 30.0% | |
| GGTTCTATCAAGATATTTGA+TGG | + | Chr2:19544396-19544415 | MsG0280007716.01.T01:CDS | 30.0% | |
| TACCAATTTCTACTCCATAA+GGG | - | Chr2:19543228-19543247 | None:intergenic | 30.0% | |
| TCAAATATCTTGATAGAACC+TGG | - | Chr2:19544396-19544415 | None:intergenic | 30.0% | |
| TTAAGTCCAAATTACAAAGG+AGG | + | Chr2:19543397-19543416 | MsG0280007716.01.T01:CDS | 30.0% | |
| TTACCAATTTCTACTCCATA+AGG | - | Chr2:19543229-19543248 | None:intergenic | 30.0% | |
| TTATGCCAAGATGATATTCT+TGG | + | Chr2:19543148-19543167 | MsG0280007716.01.T01:CDS | 30.0% | |
| ! | AATGGGACATTTCTATTTCT+TGG | + | Chr2:19544473-19544492 | MsG0280007716.01.T01:CDS | 30.0% |
| ! | CATTTTTCATCATACCCTTT+AGG | - | Chr2:19543265-19543284 | None:intergenic | 30.0% |
| ! | CTAAAGGGTATGATGAAAAA+TGG | + | Chr2:19543263-19543282 | MsG0280007716.01.T01:CDS | 30.0% |
| ! | TGAGGTTTTTATCTCAAGAT+TGG | + | Chr2:19542786-19542805 | MsG0280007716.01.T01:CDS | 30.0% |
| !!! | CGCTGATTTAACATTGTTTT+TGG | - | Chr2:19542882-19542901 | None:intergenic | 30.0% |
| !!! | GGTATTTTAGAACAATCCAA+TGG | - | Chr2:19543066-19543085 | None:intergenic | 30.0% |
| !!! | TTGTCACATTTTTAGGTTGT+TGG | - | Chr2:19543087-19543106 | None:intergenic | 30.0% |
| ACAAGTGTCCCAATAAAAAC+TGG | + | Chr2:19543791-19543810 | MsG0280007716.01.T01:CDS | 35.0% | |
| ACATATATTCCTGACCCTTA+TGG | + | Chr2:19543211-19543230 | MsG0280007716.01.T01:CDS | 35.0% | |
| ACTCACTGTGCTTGATATAT+AGG | + | Chr2:19543700-19543719 | MsG0280007716.01.T01:intron | 35.0% | |
| ATAGAAATGTCCCATTACAC+CGG | - | Chr2:19544469-19544488 | None:intergenic | 35.0% | |
| ATCTTGATAGAACCTGGTTT+AGG | - | Chr2:19544390-19544409 | None:intergenic | 35.0% | |
| ATGACATACCTGTCCATATA+GGG | - | Chr2:19543870-19543889 | None:intergenic | 35.0% | |
| ATGTCCATGCAATTGATACA+AGG | + | Chr2:19543293-19543312 | MsG0280007716.01.T01:CDS | 35.0% | |
| ATGTTTGATGCTCATTCCAT+TGG | - | Chr2:19543596-19543615 | None:intergenic | 35.0% | |
| CCAAATTACAAAGGAGGTTT+AGG | + | Chr2:19543403-19543422 | MsG0280007716.01.T01:CDS | 35.0% | |
| CCCACATAATTGTGTACTTT+AGG | - | Chr2:19543495-19543514 | None:intergenic | 35.0% | |
| CCTAAACCTCCTTTGTAATT+TGG | - | Chr2:19543406-19543425 | None:intergenic | 35.0% | |
| CCTAAGATTGAATTAAGTCC+AGG | + | Chr2:19542914-19542933 | MsG0280007716.01.T01:CDS | 35.0% | |
| CCTGGACTTAATTCAATCTT+AGG | - | Chr2:19542917-19542936 | None:intergenic | 35.0% | |
| CTAGTTGAACCAAATTCAAC+CGG | + | Chr2:19544113-19544132 | MsG0280007716.01.T01:intron | 35.0% | |
| GAGCATCAAACATAATTGCA+AGG | + | Chr2:19543603-19543622 | MsG0280007716.01.T01:CDS | 35.0% | |
| GCTGAACTAGTAGATGAATA+TGG | + | Chr2:19543007-19543026 | MsG0280007716.01.T01:CDS | 35.0% | |
| GGTTATATAATCTACAGTGC+TGG | + | Chr2:19543815-19543834 | MsG0280007716.01.T01:CDS | 35.0% | |
| TAGGTAAAGGACATGTTTAG+TGG | - | Chr2:19543476-19543495 | None:intergenic | 35.0% | |
| TCAAGATTGGATGCTTTCAT+GGG | + | Chr2:19542799-19542818 | MsG0280007716.01.T01:CDS | 35.0% | |
| TCAATCCCAAAATATGGAAC+TGG | + | Chr2:19544312-19544331 | MsG0280007716.01.T01:CDS | 35.0% | |
| TCTTGATAGAACCTGGTTTA+GGG | - | Chr2:19544389-19544408 | None:intergenic | 35.0% | |
| TGAAGATAAGTTTCTTGGAG+AGG | - | Chr2:19543043-19543062 | None:intergenic | 35.0% | |
| TGCAACTCAATCCCAAAATA+TGG | + | Chr2:19544306-19544325 | MsG0280007716.01.T01:CDS | 35.0% | |
| TGTCCATGCAATTGATACAA+GGG | + | Chr2:19543294-19543313 | MsG0280007716.01.T01:CDS | 35.0% | |
| TGTGGCATTAATGTTAGGAT+GGG | + | Chr2:19544274-19544293 | MsG0280007716.01.T01:intron | 35.0% | |
| TGTTTGATGCTCATTCCATT+GGG | - | Chr2:19543595-19543614 | None:intergenic | 35.0% | |
| TTAGGATGTTGTCCTGATAA+TGG | + | Chr2:19543421-19543440 | MsG0280007716.01.T01:CDS | 35.0% | |
| TTGGTTGTGGCATTAATGTT+AGG | + | Chr2:19544269-19544288 | MsG0280007716.01.T01:intron | 35.0% | |
| TTGTGGCATTAATGTTAGGA+TGG | + | Chr2:19544273-19544292 | MsG0280007716.01.T01:intron | 35.0% | |
| ! | CCATTCCAGTTCCATATTTT+GGG | - | Chr2:19544320-19544339 | None:intergenic | 35.0% |
| ! | TACCCTTTAGGAATTTGTGA+AGG | - | Chr2:19543253-19543272 | None:intergenic | 35.0% |
| ! | TAGATTGAGGAAAGGCTTTT+TGG | + | Chr2:19543450-19543469 | MsG0280007716.01.T01:CDS | 35.0% |
| ! | TCCATTCCAGTTCCATATTT+TGG | - | Chr2:19544321-19544340 | None:intergenic | 35.0% |
| !! | AATGAGAAAGGGTATCTTGT+AGG | + | Chr2:19544348-19544367 | MsG0280007716.01.T01:CDS | 35.0% |
| !! | ACACAATTATGTGGGTTGAT+TGG | + | Chr2:19543500-19543519 | MsG0280007716.01.T01:CDS | 35.0% |
| !! | CACAATTATGTGGGTTGATT+GGG | + | Chr2:19543501-19543520 | MsG0280007716.01.T01:CDS | 35.0% |
| AAACCAATGGACGAATTGGT+CGG | - | Chr2:19544073-19544092 | None:intergenic | 40.0% | |
| AACAACAGCATAATGCACAC+CGG | + | Chr2:19544447-19544466 | MsG0280007716.01.T01:CDS | 40.0% | |
| AGTGTCCCAATAAAAACTGG+TGG | + | Chr2:19543794-19543813 | MsG0280007716.01.T01:CDS | 40.0% | |
| ATAATGCACACCGGTGTAAT+GGG | + | Chr2:19544456-19544475 | MsG0280007716.01.T01:CDS | 40.0% | |
| ATGGAAGCAGGAAATGAGAA+AGG | + | Chr2:19544336-19544355 | MsG0280007716.01.T01:CDS | 40.0% | |
| ATTTCTACTCCATAAGGGTC+AGG | - | Chr2:19543223-19543242 | None:intergenic | 40.0% | |
| CATGACATACCTGTCCATAT+AGG | - | Chr2:19543871-19543890 | None:intergenic | 40.0% | |
| CCCAAAATATGGAACTGGAA+TGG | + | Chr2:19544317-19544336 | MsG0280007716.01.T01:CDS | 40.0% | |
| CTCAAGATTGGATGCTTTCA+TGG | + | Chr2:19542798-19542817 | MsG0280007716.01.T01:CDS | 40.0% | |
| GACCCTTATGGAGTAGAAAT+TGG | + | Chr2:19543223-19543242 | MsG0280007716.01.T01:CDS | 40.0% | |
| GATCAAACCAATGGACGAAT+TGG | - | Chr2:19544077-19544096 | None:intergenic | 40.0% | |
| GCATTAATGTTAGGATGGGA+GGG | + | Chr2:19544278-19544297 | MsG0280007716.01.T01:intron | 40.0% | |
| GCGATTAGATCAACCCTATA+TGG | + | Chr2:19543854-19543873 | MsG0280007716.01.T01:CDS | 40.0% | |
| GGTCAATGTAGATTGAGGAA+AGG | + | Chr2:19543442-19543461 | MsG0280007716.01.T01:CDS | 40.0% | |
| GTCCATGCAATTGATACAAG+GGG | + | Chr2:19543295-19543314 | MsG0280007716.01.T01:CDS | 40.0% | |
| TAGATCAACCCTATATGGAC+AGG | + | Chr2:19543859-19543878 | MsG0280007716.01.T01:CDS | 40.0% | |
| TGGAAGCAGGAAATGAGAAA+GGG | + | Chr2:19544337-19544356 | MsG0280007716.01.T01:CDS | 40.0% | |
| TTATGTGTGATTCATGCGAC+TGG | - | Chr2:19543934-19543953 | None:intergenic | 40.0% | |
| TTGAGTGCAATATGACCCTT+TGG | - | Chr2:19542977-19542996 | None:intergenic | 40.0% | |
| ! | GGGACATTTCTATTTCTTGG+TGG | + | Chr2:19544476-19544495 | MsG0280007716.01.T01:CDS | 40.0% |
| ! | GGTTGATTGGGATGATTTTG+TGG | + | Chr2:19543513-19543532 | MsG0280007716.01.T01:CDS | 40.0% |
| !! | CTGTTGGTAGCTAATAAGCA+TGG | - | Chr2:19542854-19542873 | None:intergenic | 40.0% |
| AAAAGCCATGGGAATGGTAG+TGG | + | Chr2:19543749-19543768 | MsG0280007716.01.T01:CDS | 45.0% | |
| AAACCTTGCACCAAAAGCCA+TGG | + | Chr2:19543737-19543756 | MsG0280007716.01.T01:CDS | 45.0% | |
| AACCTTGCACCAAAAGCCAT+GGG | + | Chr2:19543738-19543757 | MsG0280007716.01.T01:CDS | 45.0% | |
| ATCGAACCAAGTACCAACCA+CGG | - | Chr2:19544143-19544162 | None:intergenic | 45.0% | |
| CACCCCTTGTATCAATTGCA+TGG | - | Chr2:19543300-19543319 | None:intergenic | 45.0% | |
| CATAATGCACACCGGTGTAA+TGG | + | Chr2:19544455-19544474 | MsG0280007716.01.T01:CDS | 45.0% | |
| CTTGGACAATACTATGGCCT+AGG | + | Chr2:19543166-19543185 | MsG0280007716.01.T01:CDS | 45.0% | |
| GGCATTAATGTTAGGATGGG+AGG | + | Chr2:19544277-19544296 | MsG0280007716.01.T01:intron | 45.0% | |
| TATGGAACTGGAATGGAAGC+AGG | + | Chr2:19544324-19544343 | MsG0280007716.01.T01:CDS | 45.0% | |
| TGCAATTGATACAAGGGGTG+TGG | + | Chr2:19543300-19543319 | MsG0280007716.01.T01:CDS | 45.0% | |
| TTGGATGCTTTCATGGGCAA+TGG | + | Chr2:19542805-19542824 | MsG0280007716.01.T01:CDS | 45.0% | |
| ! | AAGGGGTGTGGAAGATAGAA+TGG | + | Chr2:19543312-19543331 | MsG0280007716.01.T01:CDS | 45.0% |
| ! | ACTACCATTCCCATGGCTTT+TGG | - | Chr2:19543750-19543769 | None:intergenic | 45.0% |
| ! | AGGGGTGTGGAAGATAGAAT+GGG | + | Chr2:19543313-19543332 | MsG0280007716.01.T01:CDS | 45.0% |
| !! | AGTGCTGGTCATCAACATGT+AGG | + | Chr2:19543830-19543849 | MsG0280007716.01.T01:CDS | 45.0% |
| !! | TCAACTTGCTACCCTAAACC+AGG | + | Chr2:19544375-19544394 | MsG0280007716.01.T01:CDS | 45.0% |
| AAGTACCAACCACGGAACAC+CGG | - | Chr2:19544135-19544154 | None:intergenic | 50.0% | |
| CAAATTCAACCGGTGTTCCG+TGG | + | Chr2:19544123-19544142 | MsG0280007716.01.T01:intron | 50.0% | |
| CACAACCACTACCATTCCCA+TGG | - | Chr2:19543757-19543776 | None:intergenic | 50.0% | |
| GGGGTGTGGAAGATAGAATG+GGG | + | Chr2:19543314-19543333 | MsG0280007716.01.T01:CDS | 50.0% | |
| TGCACCAAAAGCCATGGGAA+TGG | + | Chr2:19543743-19543762 | MsG0280007716.01.T01:CDS | 50.0% | |
| ! | ATGGGCAATGGTGCTAGTGT+TGG | + | Chr2:19542817-19542836 | MsG0280007716.01.T01:CDS | 50.0% |
| ! | CACGGAACACCGGTTGAATT+TGG | - | Chr2:19544125-19544144 | None:intergenic | 50.0% |
| ! | TGCCTCTAGTTTCAGCTCCT+AGG | - | Chr2:19543186-19543205 | None:intergenic | 50.0% |
| !! | TTCCCATGGCTTTTGGTGCA+AGG | - | Chr2:19543743-19543762 | None:intergenic | 50.0% |
| GAGCCGACCAATTCGTCCAT+TGG | + | Chr2:19544067-19544086 | MsG0280007716.01.T01:intron | 55.0% | |
| GGCCTAGGAGCTGAAACTAG+AGG | + | Chr2:19543181-19543200 | MsG0280007716.01.T01:CDS | 55.0% | |
| TTCAACCGGTGTTCCGTGGT+TGG | + | Chr2:19544127-19544146 | MsG0280007716.01.T01:intron | 55.0% | |
| ! | GGTGTTCCGTGGTTGGTACT+TGG | + | Chr2:19544134-19544153 | MsG0280007716.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 19542785 | 19544527 | 19542785 | ID=MsG0280007716.01;Name=MsG0280007716.01 |
| Chr2 | mRNA | 19542785 | 19544527 | 19542785 | ID=MsG0280007716.01.T01;Parent=MsG0280007716.01;Name=MsG0280007716.01.T01;_AED=0.45;_eAED=0.45;_QI=0|0|0|1|1|1|3|0|411 |
| Chr2 | exon | 19542785 | 19543624 | 19542785 | ID=MsG0280007716.01.T01:exon:10181;Parent=MsG0280007716.01.T01 |
| Chr2 | exon | 19543722 | 19543880 | 19543722 | ID=MsG0280007716.01.T01:exon:10182;Parent=MsG0280007716.01.T01 |
| Chr2 | exon | 19544291 | 19544527 | 19544291 | ID=MsG0280007716.01.T01:exon:10183;Parent=MsG0280007716.01.T01 |
| Chr2 | CDS | 19542785 | 19543624 | 19542785 | ID=MsG0280007716.01.T01:cds;Parent=MsG0280007716.01.T01 |
| Chr2 | CDS | 19543722 | 19543880 | 19543722 | ID=MsG0280007716.01.T01:cds;Parent=MsG0280007716.01.T01 |
| Chr2 | CDS | 19544291 | 19544527 | 19544291 | ID=MsG0280007716.01.T01:cds;Parent=MsG0280007716.01.T01 |
| Gene Sequence |
| Protein sequence |
