AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006519.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006519.01.T01 MTR_2g010000 98.571 70 1 0 1 70 10 79 3.92e-44 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTATATATTTGATGGTT+TGG 0.264995 2:-3033095 MsG0280006519.01.T01:CDS
GCTTATATATTTGATGGTTT+GGG 0.270345 2:-3033094 MsG0280006519.01.T01:CDS
CCCTTCTCATCCTGTAGGTT+TGG 0.326308 2:-3033128 MsG0280006519.01.T01:CDS
AAGAGAGCTTATATATTTGA+TGG 0.334219 2:-3033100 MsG0280006519.01.T01:CDS
GACAACTTCTGATTCCCTTT+TGG 0.370789 2:+3032960 None:intergenic
GGCATAGAGTAATGGAGAAT+GGG 0.391709 2:-3033201 None:intergenic
AGGCATAGAGTAATGGAGAA+TGG 0.405499 2:-3033202 None:intergenic
TTATAATAAGGAGTGGGATC+TGG 0.412024 2:-3033065 MsG0280006519.01.T01:CDS
GGTCGAGGGAATGAGTTTCT+TGG 0.441250 2:-3032998 MsG0280006519.01.T01:CDS
TCGGATGCGGTGGAAGGTCG+AGG 0.444508 2:-3033013 MsG0280006519.01.T01:CDS
CTCTTCTTCACCAAACCTAC+AGG 0.483363 2:+3033118 None:intergenic
TACCATGTGGAACTTCCAAA+AGG 0.493259 2:-3032975 MsG0280006519.01.T01:CDS
GCATAGAGTAATGGAGAATG+GGG 0.528940 2:-3033200 None:intergenic
AAGGAATCGGATGCGGTGGA+AGG 0.539802 2:-3033019 MsG0280006519.01.T01:CDS
GAAGTTGTCGCAATCTGCGC+AGG 0.544207 2:-3032946 MsG0280006519.01.T01:CDS
TTCTACCCTTCTCATCCTGT+AGG 0.545495 2:-3033133 MsG0280006519.01.T01:CDS
CTTATATATTTGATGGTTTG+GGG 0.551017 2:-3033093 MsG0280006519.01.T01:CDS
ACCATGTGGAACTTCCAAAA+GGG 0.569430 2:-3032974 MsG0280006519.01.T01:CDS
ACCAAACCTACAGGATGAGA+AGG 0.574351 2:+3033127 None:intergenic
AGAAGAAAAGGAATCGGATG+CGG 0.601504 2:-3033026 MsG0280006519.01.T01:CDS
TAGAATCTATGCTCATCCAC+AGG 0.612054 2:+3033151 None:intergenic
AGAAAAGGAATCGGATGCGG+TGG 0.616588 2:-3033023 MsG0280006519.01.T01:CDS
CGGATGCGGTGGAAGGTCGA+GGG 0.630729 2:-3033012 MsG0280006519.01.T01:CDS
ATGAGTTTCTTGGTACCATG+TGG 0.648410 2:-3032988 MsG0280006519.01.T01:CDS
CCAAACCTACAGGATGAGAA+GGG 0.654788 2:+3033128 None:intergenic
GATGGATGTTGAAAATCCTG+TGG 0.709912 2:-3033167 MsG0280006519.01.T01:CDS
CATAGAGTAATGGAGAATGG+GGG 0.717384 2:-3033199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTTATCAAAAAGAAGAAA+AGG - Chr2:3033092-3033111 MsG0280006519.01.T01:CDS 20.0%
!!! GAATTTTTATAATAAGGAGT+GGG - Chr2:3033059-3033078 MsG0280006519.01.T01:CDS 20.0%
!!! TTTGGGGAATTTTTATAATA+AGG - Chr2:3033053-3033072 MsG0280006519.01.T01:CDS 20.0%
! AAGAGAGCTTATATATTTGA+TGG - Chr2:3033030-3033049 MsG0280006519.01.T01:CDS 25.0%
! TCAAAAAGAAGAAAAGGAAT+CGG - Chr2:3033098-3033117 MsG0280006519.01.T01:CDS 25.0%
!! AGCTTATATATTTGATGGTT+TGG - Chr2:3033035-3033054 MsG0280006519.01.T01:CDS 25.0%
!! CTTATATATTTGATGGTTTG+GGG - Chr2:3033037-3033056 MsG0280006519.01.T01:CDS 25.0%
!! GCTTATATATTTGATGGTTT+GGG - Chr2:3033036-3033055 MsG0280006519.01.T01:CDS 25.0%
!! GGAATTTTTATAATAAGGAG+TGG - Chr2:3033058-3033077 MsG0280006519.01.T01:CDS 25.0%
TTATAATAAGGAGTGGGATC+TGG - Chr2:3033065-3033084 MsG0280006519.01.T01:CDS 35.0%
ACCATGTGGAACTTCCAAAA+GGG - Chr2:3033156-3033175 MsG0280006519.01.T01:CDS 40.0%
AGAAGAAAAGGAATCGGATG+CGG - Chr2:3033104-3033123 MsG0280006519.01.T01:CDS 40.0%
ATGAGTTTCTTGGTACCATG+TGG - Chr2:3033142-3033161 MsG0280006519.01.T01:CDS 40.0%
GATGGATGTTGAAAATCCTG+TGG - Chr2:3032963-3032982 MsG0280006519.01.T01:CDS 40.0%
TACCATGTGGAACTTCCAAA+AGG - Chr2:3033155-3033174 MsG0280006519.01.T01:CDS 40.0%
TAGAATCTATGCTCATCCAC+AGG + Chr2:3032982-3033001 None:intergenic 40.0%
! GACAACTTCTGATTCCCTTT+TGG + Chr2:3033173-3033192 None:intergenic 40.0%
ACCAAACCTACAGGATGAGA+AGG + Chr2:3033006-3033025 None:intergenic 45.0%
CCAAACCTACAGGATGAGAA+GGG + Chr2:3033005-3033024 None:intergenic 45.0%
CTCTTCTTCACCAAACCTAC+AGG + Chr2:3033015-3033034 None:intergenic 45.0%
TTCTACCCTTCTCATCCTGT+AGG - Chr2:3032997-3033016 MsG0280006519.01.T01:CDS 45.0%
!! TCCCTTTTGGAAGTTCCACA+TGG + Chr2:3033160-3033179 None:intergenic 45.0%
AGAAAAGGAATCGGATGCGG+TGG - Chr2:3033107-3033126 MsG0280006519.01.T01:CDS 50.0%
CCCTTCTCATCCTGTAGGTT+TGG - Chr2:3033002-3033021 MsG0280006519.01.T01:CDS 50.0%
GGTCGAGGGAATGAGTTTCT+TGG - Chr2:3033132-3033151 MsG0280006519.01.T01:CDS 50.0%
AAGGAATCGGATGCGGTGGA+AGG - Chr2:3033111-3033130 MsG0280006519.01.T01:CDS 55.0%
GAAGTTGTCGCAATCTGCGC+AGG - Chr2:3033184-3033203 MsG0280006519.01.T01:CDS 55.0%
GAATGGGGGAGCTGAGGAGA+TGG - Chr2:3032945-3032964 MsG0280006519.01.T01:CDS 60.0%
CGGATGCGGTGGAAGGTCGA+GGG - Chr2:3033118-3033137 MsG0280006519.01.T01:CDS 65.0%
TCGGATGCGGTGGAAGGTCG+AGG - Chr2:3033117-3033136 MsG0280006519.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 3032940 3033212 3032940 ID=MsG0280006519.01;Name=MsG0280006519.01
Chr2 mRNA 3032940 3033212 3032940 ID=MsG0280006519.01.T01;Parent=MsG0280006519.01;Name=MsG0280006519.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|90
Chr2 exon 3032940 3033212 3032940 ID=MsG0280006519.01.T01:exon:579;Parent=MsG0280006519.01.T01
Chr2 CDS 3032940 3033212 3032940 ID=MsG0280006519.01.T01:cds;Parent=MsG0280006519.01.T01
Gene Sequence

>MsG0280006519.01.T01

ATGGAGAATGGGGGAGCTGAGGAGATGGATGTTGAAAATCCTGTGGATGAGCATAGATTCTACCCTTCTCATCCTGTAGGTTTGGTGAAGAAGAGAGCTTATATATTTGATGGTTTGGGGAATTTTTATAATAAGGAGTGGGATCTGGCTGATCTTTATCAAAAAGAAGAAAAGGAATCGGATGCGGTGGAAGGTCGAGGGAATGAGTTTCTTGGTACCATGTGGAACTTCCAAAAGGGAATCAGAAGTTGTCGCAATCTGCGCAGGACTTGA

Protein sequence

>MsG0280006519.01.T01

MENGGAEEMDVENPVDEHRFYPSHPVGLVKKRAYIFDGLGNFYNKEWDLADLYQKEEKESDAVEGRGNEFLGTMWNFQKGIRSCRNLRRT*