AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042795.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042795.01.T01 MTR_8g022330 63.248 117 15 2 6 95 56 171 6.70e-42 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042795.01.T01 AT1G17350 49.153 118 31 3 6 95 56 172 7.29e-30 106
MsG0880042795.01.T01 AT1G17350 49.153 118 31 3 6 95 56 172 7.29e-30 106
MsG0880042795.01.T01 AT1G17350 49.153 118 31 3 6 95 56 172 7.29e-30 106
MsG0880042795.01.T01 AT1G17350 49.153 118 31 3 6 95 56 172 7.29e-30 106
MsG0880042795.01.T01 AT1G17350 50.442 113 28 2 6 90 56 168 1.01e-29 106
MsG0880042795.01.T01 AT1G72420 47.863 117 32 3 6 94 56 171 6.62e-26 96.3
MsG0880042795.01.T01 AT1G72420 47.863 117 32 3 6 94 56 171 6.62e-26 96.3
MsG0880042795.01.T01 AT1G72420 47.863 117 32 3 6 94 45 160 6.91e-26 95.9
MsG0880042795.01.T01 AT1G72420 60.784 51 19 1 6 55 56 106 8.56e-14 63.2
MsG0880042795.01.T01 AT1G72420 60.784 51 19 1 6 55 56 106 8.56e-14 63.2
MsG0880042795.01.T01 AT1G72420 60.784 51 19 1 6 55 56 106 1.24e-13 63.5

Find 25 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAATATACCAGTTATCTT+TGG 0.216625 8:+14143256 None:intergenic
TTCAAATTGTTATGTTGTTC+AGG 0.236156 8:-14144436 None:intergenic
CAGATACCTGAATCTGAAAA+TGG 0.295053 8:-14144394 MsG0880042795.01.T01:CDS
TGCAGATATACACGGAGAAT+TGG 0.328795 8:-14143333 MsG0880042795.01.T01:intron
GCAATATACCAGTTATCTTT+GGG 0.348274 8:+14143257 None:intergenic
TTGTGGAATGCGCTCCAAAA+AGG 0.350095 8:-14144290 MsG0880042795.01.T01:intron
GTTACGTGCCCAAAGATAAC+TGG 0.367548 8:-14143265 MsG0880042795.01.T01:CDS
CATATCAATCATTACTAATC+AGG 0.379218 8:+14143199 None:intergenic
TGTAAGCAGTAAGATGGAAA+TGG 0.427922 8:+14143223 None:intergenic
TCTGAAAATGGTAAAACTAC+TGG 0.430983 8:-14144382 MsG0880042795.01.T01:CDS
CTCTGGGAATCTTTCACTTG+AGG 0.435126 8:-14144353 MsG0880042795.01.T01:CDS
GCAGATATACACGGAGAATT+GGG 0.459883 8:-14143332 MsG0880042795.01.T01:intron
TCAGGTTGTAAGCAGTAAGA+TGG 0.513936 8:+14143217 None:intergenic
GTAAGCAGTAAGATGGAAAT+GGG 0.514535 8:+14143224 None:intergenic
GAGTTGTCCTCCATCTGTCC+AGG 0.525035 8:+14143301 None:intergenic
CTTTCACTTGAGGTTACTCA+AGG 0.540859 8:-14144343 MsG0880042795.01.T01:CDS
GGTTAACTCACCTGGACAGA+TGG 0.543070 8:-14143311 MsG0880042795.01.T01:CDS
AAGTGGAATATTAGTAGAGG+TGG 0.553563 8:-14144316 MsG0880042795.01.T01:CDS
GCTAAGTGGAATATTAGTAG+AGG 0.567692 8:-14144319 MsG0880042795.01.T01:CDS
GACAGATGGAGGACAACTCA+TGG 0.575304 8:-14143297 MsG0880042795.01.T01:CDS
GAGAATTGGGTTAACTCACC+TGG 0.591802 8:-14143319 MsG0880042795.01.T01:CDS
AGGTTACTCAAGGTGCTAAG+TGG 0.593770 8:-14144333 MsG0880042795.01.T01:CDS
AGATAACTGGTATATTGCAA+AGG 0.608700 8:-14143252 MsG0880042795.01.T01:CDS
TAACTCACCTGGACAGATGG+AGG 0.644910 8:-14143308 MsG0880042795.01.T01:CDS
GATTATGGTGCAGATATACA+CGG 0.690928 8:-14143341 MsG0880042795.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTTGATATAGAAAT+AGG + Chr8:14143476-14143495 None:intergenic 10.0%
!! TTTAAATAATCAATTCAAAC+AGG - Chr8:14143635-14143654 MsG0880042795.01.T01:intron 15.0%
!! ATTGAATTGAATTGTATGAT+TGG - Chr8:14144033-14144052 MsG0880042795.01.T01:intron 20.0%
!!! CATTTGGCATTTTAAAAAAA+AGG + Chr8:14144214-14144233 None:intergenic 20.0%
! AAATTTCCTAGGATATCATT+TGG + Chr8:14144230-14144249 None:intergenic 25.0%
! AATTCAATTCAATATGCACT+AGG + Chr8:14144026-14144045 None:intergenic 25.0%
! AGCTAATAAGTCAATTCAAA+CGG - Chr8:14143973-14143992 MsG0880042795.01.T01:intron 25.0%
! CAAGATAACTTATAGCTTAT+AGG + Chr8:14143517-14143536 None:intergenic 25.0%
! TATGCATTGAATTGTATGAA+TGG - Chr8:14143698-14143717 MsG0880042795.01.T01:intron 25.0%
! TATTGTAACTAGTTGGATTA+TGG - Chr8:14144287-14144306 MsG0880042795.01.T01:intron 25.0%
! TTTAAAAAAAAGGACACTCA+CGG + Chr8:14144204-14144223 None:intergenic 25.0%
!! ATTAGCTTATCTAATGGATA+AGG + Chr8:14143960-14143979 None:intergenic 25.0%
!! TGACTTATTAGCTTATCTAA+TGG + Chr8:14143966-14143985 None:intergenic 25.0%
AAAATGCCAAATGATATCCT+AGG - Chr8:14144221-14144240 MsG0880042795.01.T01:intron 30.0%
AAGTTATCTTGTAGAGCTTA+TGG - Chr8:14143526-14143545 MsG0880042795.01.T01:intron 30.0%
ACGATAGCTATGAAACTTAA+AGG - Chr8:14144155-14144174 MsG0880042795.01.T01:intron 30.0%
AGATAACTGGTATATTGCAA+AGG - Chr8:14144391-14144410 MsG0880042795.01.T01:CDS 30.0%
GATATCCTAGGAAATTTAGA+GGG - Chr8:14144233-14144252 MsG0880042795.01.T01:intron 30.0%
GCAATATACCAGTTATCTTT+GGG + Chr8:14144389-14144408 None:intergenic 30.0%
TCTGAAAATGGTAAAACTAC+TGG - Chr8:14143261-14143280 MsG0880042795.01.T01:CDS 30.0%
TGATATCCTAGGAAATTTAG+AGG - Chr8:14144232-14144251 MsG0880042795.01.T01:intron 30.0%
TGCAATATACCAGTTATCTT+TGG + Chr8:14144390-14144409 None:intergenic 30.0%
TTGAGTGATTATAGTGTAAG+CGG - Chr8:14143770-14143789 MsG0880042795.01.T01:intron 30.0%
! AAAACTACTGGAATTTTCTC+TGG - Chr8:14143273-14143292 MsG0880042795.01.T01:CDS 30.0%
! AAACTACTGGAATTTTCTCT+GGG - Chr8:14143274-14143293 MsG0880042795.01.T01:CDS 30.0%
! AGTTAAAAAAGCCACCTTTT+TGG + Chr8:14143370-14143389 None:intergenic 30.0%
! GTTTTACCATTTTCAGATTC+AGG + Chr8:14143258-14143277 None:intergenic 30.0%
! TTGCAAGTATTGTAACTAGT+TGG - Chr8:14144280-14144299 MsG0880042795.01.T01:intron 30.0%
!! TCTGTGTTTTTGTAGTTTGA+TGG - Chr8:14144109-14144128 MsG0880042795.01.T01:intron 30.0%
!! TTCTCATTTTTGTTCAACGA+CGG - Chr8:14144063-14144082 MsG0880042795.01.T01:intron 30.0%
AAGTGGAATATTAGTAGAGG+TGG - Chr8:14143327-14143346 MsG0880042795.01.T01:CDS 35.0%
AAGTTCGACAACAACATAGA+TGG - Chr8:14143667-14143686 MsG0880042795.01.T01:intron 35.0%
AGGGTTGAAGTTTCATTTCA+TGG - Chr8:14144252-14144271 MsG0880042795.01.T01:intron 35.0%
CAAAAACACAGAATTGCGTA+AGG + Chr8:14144101-14144120 None:intergenic 35.0%
CAGATACCTGAATCTGAAAA+TGG - Chr8:14143249-14143268 MsG0880042795.01.T01:CDS 35.0%
CTCAAAGACTTTAGTGTTGT+TGG - Chr8:14143388-14143407 MsG0880042795.01.T01:intron 35.0%
GATTATGGTGCAGATATACA+CGG - Chr8:14144302-14144321 MsG0880042795.01.T01:CDS 35.0%
GCTAAGTGGAATATTAGTAG+AGG - Chr8:14143324-14143343 MsG0880042795.01.T01:CDS 35.0%
GCTATGAAACTTAAAGGAGA+TGG - Chr8:14144161-14144180 MsG0880042795.01.T01:intron 35.0%
GTAAGCAGTAAGATGGAAAT+GGG + Chr8:14144422-14144441 None:intergenic 35.0%
TGTAAGCAGTAAGATGGAAA+TGG + Chr8:14144423-14144442 None:intergenic 35.0%
TTCAACCCTCTAAATTTCCT+AGG + Chr8:14144241-14144260 None:intergenic 35.0%
TTGTTGTCGAACTTGAACAA+AGG + Chr8:14143661-14143680 None:intergenic 35.0%
! CTCAATGATTTTGCAGTGTT+TGG - Chr8:14143736-14143755 MsG0880042795.01.T01:intron 35.0%
! CTCACAAGTTTTTATGCTAG+TGG - Chr8:14143607-14143626 MsG0880042795.01.T01:intron 35.0%
!! ATTAGTAGAGGTGGATTTTG+TGG - Chr8:14143336-14143355 MsG0880042795.01.T01:intron 35.0%
AAGGAGATGTGAGACTCTAT+GGG + Chr8:14143941-14143960 None:intergenic 40.0%
CTTTCACTTGAGGTTACTCA+AGG - Chr8:14143300-14143319 MsG0880042795.01.T01:CDS 40.0%
GCAGATATACACGGAGAATT+GGG - Chr8:14144311-14144330 MsG0880042795.01.T01:CDS 40.0%
TAAGGAGATGTGAGACTCTA+TGG + Chr8:14143942-14143961 None:intergenic 40.0%
TGCAGATATACACGGAGAAT+TGG - Chr8:14144310-14144329 MsG0880042795.01.T01:CDS 40.0%
! GTTTGATGGCTTCATTGACT+TGG - Chr8:14144123-14144142 MsG0880042795.01.T01:intron 40.0%
! TCAGGTTGTAAGCAGTAAGA+TGG + Chr8:14144429-14144448 None:intergenic 40.0%
AGGAGATGTGAGACTCTATG+GGG + Chr8:14143940-14143959 None:intergenic 45.0%
GAGAATTGGGTTAACTCACC+TGG - Chr8:14144324-14144343 MsG0880042795.01.T01:CDS 45.0%
GTTACGTGCCCAAAGATAAC+TGG - Chr8:14144378-14144397 MsG0880042795.01.T01:CDS 45.0%
TTGTGGAATGCGCTCCAAAA+AGG - Chr8:14143353-14143372 MsG0880042795.01.T01:intron 45.0%
! AGGTTACTCAAGGTGCTAAG+TGG - Chr8:14143310-14143329 MsG0880042795.01.T01:CDS 45.0%
! CTCTGGGAATCTTTCACTTG+AGG - Chr8:14143290-14143309 MsG0880042795.01.T01:CDS 45.0%
GACAGATGGAGGACAACTCA+TGG - Chr8:14144346-14144365 MsG0880042795.01.T01:CDS 50.0%
GGAGATGTGAGACTCTATGG+GGG + Chr8:14143939-14143958 None:intergenic 50.0%
GGTTAACTCACCTGGACAGA+TGG - Chr8:14144332-14144351 MsG0880042795.01.T01:CDS 50.0%
TAACTCACCTGGACAGATGG+AGG - Chr8:14144335-14144354 MsG0880042795.01.T01:CDS 50.0%
TGGAATGCGCTCCAAAAAGG+TGG - Chr8:14143356-14143375 MsG0880042795.01.T01:intron 50.0%
GAGTTGTCCTCCATCTGTCC+AGG + Chr8:14144345-14144364 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 14143213 14144452 14143213 ID=MsG0880042795.01;Name=MsG0880042795.01
Chr8 mRNA 14143213 14144452 14143213 ID=MsG0880042795.01.T01;Parent=MsG0880042795.01;Name=MsG0880042795.01.T01;_AED=0.52;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|99
Chr8 exon 14144291 14144452 14144291 ID=MsG0880042795.01.T01:exon:3828;Parent=MsG0880042795.01.T01
Chr8 exon 14143213 14143350 14143213 ID=MsG0880042795.01.T01:exon:3827;Parent=MsG0880042795.01.T01
Chr8 CDS 14144291 14144452 14144291 ID=MsG0880042795.01.T01:cds;Parent=MsG0880042795.01.T01
Chr8 CDS 14143213 14143350 14143213 ID=MsG0880042795.01.T01:cds;Parent=MsG0880042795.01.T01
Gene Sequence

>MsG0880042795.01.T01

TTGTTATGTTGTTCAGGTTTGTCATCTGCATCATTGCAGATACCTGAATCTGAAAATGGTAAAACTACTGGAATTTTCTCTGGGAATCTTTCACTTGAGGTTACTCAAGGTGCTAAGTGGAATATTAGTAGAGGTGGATTTTGTGGAATGCGCTCCAAAAAGATATACACGGAGAATTGGGTTAACTCACCTGGACAGATGGAGGACAACTCATGGCAATCATTTGTTACGTGCCCAAAGATAACTGGTATATTGCAAAGGCAAGTCCCATTTCCATCTTACTGCTTACAACCTGATTAG

Protein sequence

>MsG0880042795.01.T01

LLCCSGLSSASLQIPESENGKTTGIFSGNLSLEVTQGAKWNISRGGFCGMRSKKIYTENWVNSPGQMEDNSWQSFVTCPKITGILQRQVPFPSYCLQPD*