Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009270.01.T01 | RHN74300.1 | 92.553 | 94 | 4 | 1 | 1 | 94 | 1 | 91 | 9.93E-54 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009270.01.T01 | A0A396J878 | 92.553 | 94 | 4 | 1 | 1 | 94 | 1 | 91 | 4.74e-54 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000611.01 | MsG0280009270.01 | 0.810627 | 1.049126e-50 | 6.356089e-48 |
| MsG0180001311.01 | MsG0280009270.01 | 0.842677 | 2.235072e-58 | 3.414041e-55 |
| MsG0180002185.01 | MsG0280009270.01 | 0.810144 | 1.334033e-50 | 7.979405e-48 |
| MsG0180003379.01 | MsG0280009270.01 | 0.832428 | 9.507199e-56 | 1.062277e-52 |
| MsG0180003807.01 | MsG0280009270.01 | 0.837287 | 5.681442e-57 | 7.345537e-54 |
| MsG0180004160.01 | MsG0280009270.01 | 0.806784 | 6.951020e-50 | 3.806446e-47 |
| MsG0180004437.01 | MsG0280009270.01 | 0.828700 | 7.771599e-55 | 7.783512e-52 |
| MsG0180004486.01 | MsG0280009270.01 | 0.819279 | 1.262633e-52 | 9.672915e-50 |
| MsG0180004684.01 | MsG0280009270.01 | 0.801542 | 8.574566e-49 | 4.105583e-46 |
| MsG0180005268.01 | MsG0280009270.01 | 0.819382 | 1.196036e-52 | 9.188844e-50 |
| MsG0180006025.01 | MsG0280009270.01 | 0.802691 | 4.974756e-49 | 2.452660e-46 |
| MsG0180006050.01 | MsG0280009270.01 | 0.808208 | 3.467828e-50 | 1.970947e-47 |
| MsG0280006367.01 | MsG0280009270.01 | 0.815703 | 8.070703e-52 | 5.605002e-49 |
| MsG0280006679.01 | MsG0280009270.01 | 0.811297 | 7.514002e-51 | 4.633652e-48 |
| MsG0280006852.01 | MsG0280009270.01 | 0.808904 | 2.462331e-50 | 1.425160e-47 |
| MsG0280006935.01 | MsG0280009270.01 | 0.808059 | 3.729749e-50 | 2.111376e-47 |
| MsG0280007011.01 | MsG0280009270.01 | 0.811089 | 8.332608e-51 | 5.110806e-48 |
| MsG0280007109.01 | MsG0280009270.01 | 0.811563 | 6.576495e-51 | 4.084344e-48 |
| MsG0280007691.01 | MsG0280009270.01 | 0.815746 | 7.894711e-52 | 5.489546e-49 |
| MsG0280007775.01 | MsG0280009270.01 | 0.849546 | 3.016680e-60 | 5.738025e-57 |
| MsG0280009270.01 | MsG0280009554.01 | 0.807386 | 5.183970e-50 | 2.883559e-47 |
| MsG0280009270.01 | MsG0280010547.01 | 0.810176 | 1.313035e-50 | 7.860240e-48 |
| MsG0280009270.01 | MsG0280010816.01 | 0.806665 | 7.367542e-50 | 4.021936e-47 |
| MsG0280009270.01 | MsG0280010976.01 | 0.804026 | 2.631683e-49 | 1.342663e-46 |
| MsG0280009270.01 | MsG0280011201.01 | 0.801416 | 9.097158e-49 | 4.342013e-46 |
| MsG0280009270.01 | MsG0380012161.01 | 0.805566 | 1.255003e-49 | 6.661289e-47 |
| MsG0280009270.01 | MsG0380013471.01 | 0.807111 | 5.929982e-50 | 3.274766e-47 |
| MsG0280009270.01 | MsG0380014576.01 | 0.800743 | 1.249168e-48 | 5.861564e-46 |
| MsG0280009270.01 | MsG0380015220.01 | 0.807641 | 4.577945e-50 | 2.563254e-47 |
| MsG0280009270.01 | MsG0380015607.01 | 0.807315 | 5.368231e-50 | 2.980561e-47 |
| MsG0280009270.01 | MsG0380016740.01 | 0.807303 | 5.398011e-50 | 2.996213e-47 |
| MsG0280009270.01 | MsG0380017229.01 | 0.841288 | 5.202769e-58 | 7.608482e-55 |
| MsG0280009270.01 | MsG0380017401.01 | 0.801000 | 1.107006e-48 | 5.228938e-46 |
| MsG0280009270.01 | MsG0380017433.01 | 0.812259 | 4.638551e-51 | 2.935205e-48 |
| MsG0280009270.01 | MsG0380017914.01 | -0.802241 | 6.158201e-49 | 3.001535e-46 |
| MsG0280009270.01 | MsG0380017939.01 | 0.822677 | 2.083863e-53 | 1.756166e-50 |
| MsG0280009270.01 | MsG0480018292.01 | 0.837839 | 4.102215e-57 | 5.395353e-54 |
| MsG0280009270.01 | MsG0480019289.01 | 0.800710 | 1.268533e-48 | 5.947282e-46 |
| MsG0280009270.01 | MsG0480020540.01 | 0.809666 | 1.690247e-50 | 9.984822e-48 |
| MsG0280009270.01 | MsG0480020719.01 | 0.838990 | 2.070407e-57 | 2.820177e-54 |
| MsG0280009270.01 | MsG0480022026.01 | 0.805851 | 1.093250e-49 | 5.845933e-47 |
| MsG0280009270.01 | MsG0480022203.01 | 0.815339 | 9.725329e-52 | 6.687030e-49 |
| MsG0280009270.01 | MsG0480022248.01 | 0.801410 | 9.123050e-49 | 4.353744e-46 |
| MsG0280009270.01 | MsG0480023377.01 | 0.843818 | 1.109122e-58 | 1.756092e-55 |
| MsG0280009270.01 | MsG0580024581.01 | 0.803555 | 3.296893e-49 | 1.661849e-46 |
| MsG0280009270.01 | MsG0580025922.01 | 0.830764 | 2.444386e-55 | 2.600206e-52 |
| MsG0280009270.01 | MsG0580026664.01 | 0.813180 | 2.916390e-51 | 1.891089e-48 |
| MsG0280009270.01 | MsG0580028459.01 | 0.805380 | 1.372614e-49 | 7.251080e-47 |
| MsG0280009270.01 | MsG0580028526.01 | 0.806316 | 8.728024e-50 | 4.722578e-47 |
| MsG0280009270.01 | MsG0580029208.01 | 0.825145 | 5.498287e-54 | 4.970409e-51 |
| MsG0280009270.01 | MsG0580029550.01 | 0.808206 | 3.470611e-50 | 1.972425e-47 |
| MsG0280009270.01 | MsG0580029628.01 | 0.830530 | 2.789036e-55 | 2.946206e-52 |
| MsG0280009270.01 | MsG0580029801.01 | 0.806769 | 7.004394e-50 | 3.834079e-47 |
| MsG0280009270.01 | MsG0580029919.01 | 0.815058 | 1.122800e-51 | 7.660843e-49 |
| MsG0280009270.01 | MsG0680031063.01 | 0.800045 | 1.732720e-48 | 7.987811e-46 |
| MsG0280009270.01 | MsG0680031301.01 | 0.839321 | 1.699188e-57 | 2.338142e-54 |
| MsG0280009270.01 | MsG0680031654.01 | 0.818658 | 1.747615e-52 | 1.316036e-49 |
| MsG0280009270.01 | MsG0680033926.01 | 0.809356 | 1.970099e-50 | 1.154252e-47 |
| MsG0280009270.01 | MsG0680034235.01 | 0.825707 | 4.047029e-54 | 3.717321e-51 |
| MsG0280009270.01 | MsG0680034476.01 | 0.812843 | 3.457743e-51 | 2.221905e-48 |
| MsG0280009270.01 | MsG0780036117.01 | 0.806669 | 7.353870e-50 | 4.014856e-47 |
| MsG0280009270.01 | MsG0780036633.01 | 0.820960 | 5.203475e-53 | 4.177846e-50 |
| MsG0280009270.01 | MsG0780036845.01 | 0.833211 | 6.073700e-56 | 6.947656e-53 |
| MsG0280009270.01 | MsG0780039828.01 | 0.801688 | 7.999749e-49 | 3.844495e-46 |
| MsG0280009270.01 | MsG0780040125.01 | 0.814192 | 1.746403e-51 | 1.164010e-48 |
| MsG0280009270.01 | MsG0780040389.01 | 0.810932 | 9.013382e-51 | 5.505778e-48 |
| MsG0280009270.01 | MsG0780040424.01 | 0.829820 | 4.157160e-55 | 4.303251e-52 |
| MsG0280009270.01 | MsG0780040697.01 | 0.816854 | 4.462160e-52 | 3.198015e-49 |
| MsG0280009270.01 | MsG0780040742.01 | 0.810267 | 1.254883e-50 | 7.530120e-48 |
| MsG0280009270.01 | MsG0780041258.01 | 0.803670 | 3.120407e-49 | 1.577398e-46 |
| MsG0280009270.01 | MsG0780041390.01 | 0.832174 | 1.099091e-55 | 1.218824e-52 |
| MsG0280009270.01 | MsG0880043162.01 | 0.832278 | 1.035543e-55 | 1.151829e-52 |
| MsG0280009270.01 | MsG0880043341.01 | 0.804748 | 1.861682e-49 | 9.676575e-47 |
| MsG0280009270.01 | MsG0880044409.01 | 0.822950 | 1.800310e-53 | 1.528938e-50 |
| MsG0280009270.01 | MsG0880045165.01 | 0.801952 | 7.063624e-49 | 3.417442e-46 |
| MsG0280009270.01 | MsG0880045329.01 | 0.820246 | 7.590657e-53 | 5.974189e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009270.01.T01 | MTR_2g061470 | 89.899 | 99 | 5 | 2 | 1 | 97 | 1 | 96 | 1.72e-55 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009270.01.T01 | AT4G10650 | 45.833 | 72 | 34 | 2 | 31 | 97 | 28 | 99 | 2.03e-11 | 59.7 |
Find 19 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCTCGTTCCACTTTAC+AGG | 0.231025 | 2:+49141519 | MsG0280009270.01.T01:CDS |
| TAAATGGCAGCGACGCAATT+TGG | 0.264214 | 2:+49141259 | None:intergenic |
| GCGTTATACGTGAAGGATTA+TGG | 0.272367 | 2:+49141332 | MsG0280009270.01.T01:CDS |
| CATTGCAGAAAGAATCCCTT+TGG | 0.406410 | 2:+49141387 | MsG0280009270.01.T01:CDS |
| ATTATGGAACGATCCTTTCA+TGG | 0.411084 | 2:+49141348 | MsG0280009270.01.T01:CDS |
| CGAGTTGAAGCAACCATGAA+AGG | 0.501601 | 2:-49141361 | None:intergenic |
| TGAAAGAAAGAATCGAAAGA+AGG | 0.507621 | 2:+49141302 | MsG0280009270.01.T01:CDS |
| AAATGACATTGAGAGGAGAA+TGG | 0.521380 | 2:-49141445 | None:intergenic |
| GAAGTAACGAACCTGTAAAG+TGG | 0.530617 | 2:-49141530 | None:intergenic |
| CAAAGGGATTCTTTCTGCAA+TGG | 0.532384 | 2:-49141386 | None:intergenic |
| GAATCCTCGCATCAGTGACA+TGG | 0.567312 | 2:-49141423 | None:intergenic |
| TGGCAGCGACGCAATTTGGA+AGG | 0.591327 | 2:+49141263 | MsG0280009270.01.T01:CDS |
| GGACTATGAGATCAACCAAA+GGG | 0.592573 | 2:-49141402 | None:intergenic |
| GACGCAATTTGGAAGGCGAG+TGG | 0.606245 | 2:+49141270 | MsG0280009270.01.T01:CDS |
| ACGCAATTTGGAAGGCGAGT+GGG | 0.617392 | 2:+49141271 | MsG0280009270.01.T01:CDS |
| AAAAGCAGCGTTATACGTGA+AGG | 0.625766 | 2:+49141325 | MsG0280009270.01.T01:CDS |
| TGTGAGTAAATGACATTGAG+AGG | 0.630009 | 2:-49141452 | None:intergenic |
| TAGTCCATGTCACTGATGCG+AGG | 0.661016 | 2:+49141419 | MsG0280009270.01.T01:CDS |
| TGGACTATGAGATCAACCAA+AGG | 0.680919 | 2:-49141403 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAAGAAAGAATCGAAAGA+AGG | + | Chr2:49141302-49141321 | MsG0280009270.01.T01:CDS | 30.0% | |
| AAATGACATTGAGAGGAGAA+TGG | - | Chr2:49141448-49141467 | None:intergenic | 35.0% | |
| ATTATGGAACGATCCTTTCA+TGG | + | Chr2:49141348-49141367 | MsG0280009270.01.T01:CDS | 35.0% | |
| TGTGAGTAAATGACATTGAG+AGG | - | Chr2:49141455-49141474 | None:intergenic | 35.0% | |
| AAAAGCAGCGTTATACGTGA+AGG | + | Chr2:49141325-49141344 | MsG0280009270.01.T01:CDS | 40.0% | |
| CAAAGGGATTCTTTCTGCAA+TGG | - | Chr2:49141389-49141408 | None:intergenic | 40.0% | |
| CATTGCAGAAAGAATCCCTT+TGG | + | Chr2:49141387-49141406 | MsG0280009270.01.T01:CDS | 40.0% | |
| GAAGTAACGAACCTGTAAAG+TGG | - | Chr2:49141533-49141552 | None:intergenic | 40.0% | |
| GCGTTATACGTGAAGGATTA+TGG | + | Chr2:49141332-49141351 | MsG0280009270.01.T01:CDS | 40.0% | |
| GGACTATGAGATCAACCAAA+GGG | - | Chr2:49141405-49141424 | None:intergenic | 40.0% | |
| TGGACTATGAGATCAACCAA+AGG | - | Chr2:49141406-49141425 | None:intergenic | 40.0% | |
| CACTTCTCGTTCCACTTTAC+AGG | + | Chr2:49141519-49141538 | MsG0280009270.01.T01:CDS | 45.0% | |
| ! | CGAGTTGAAGCAACCATGAA+AGG | - | Chr2:49141364-49141383 | None:intergenic | 45.0% |
| ACGCAATTTGGAAGGCGAGT+GGG | + | Chr2:49141271-49141290 | MsG0280009270.01.T01:CDS | 50.0% | |
| GAATCCTCGCATCAGTGACA+TGG | - | Chr2:49141426-49141445 | None:intergenic | 50.0% | |
| TAGTCCATGTCACTGATGCG+AGG | + | Chr2:49141419-49141438 | MsG0280009270.01.T01:CDS | 50.0% | |
| GACGCAATTTGGAAGGCGAG+TGG | + | Chr2:49141270-49141289 | MsG0280009270.01.T01:CDS | 55.0% | |
| TGGCAGCGACGCAATTTGGA+AGG | + | Chr2:49141263-49141282 | MsG0280009270.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 49141262 | 49141615 | 49141262 | ID=MsG0280009270.01;Name=MsG0280009270.01 |
| Chr2 | mRNA | 49141262 | 49141615 | 49141262 | ID=MsG0280009270.01.T01;Parent=MsG0280009270.01;Name=MsG0280009270.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|117 |
| Chr2 | exon | 49141262 | 49141615 | 49141262 | ID=MsG0280009270.01.T01:exon:20608;Parent=MsG0280009270.01.T01 |
| Chr2 | CDS | 49141262 | 49141615 | 49141262 | ID=MsG0280009270.01.T01:cds;Parent=MsG0280009270.01.T01 |
| Gene Sequence |
| Protein sequence |
