AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380011927.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011927.01.T01 MTR_1g048960 96.875 96 3 0 1 96 1 96 4.16e-64 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011927.01.T01 AT1G72020 67.010 97 31 1 1 96 1 97 1.18e-43 137

Find 19 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCCTCTACTCACTAATT+TGG 0.203441 3:+8069369 None:intergenic
CTTGGACAAGATGAAGTATC+AGG 0.339393 3:-8069266 MsG0380011927.01.T01:CDS
AGTAGAGGATTTCATTCAAC+TGG 0.411924 3:-8069358 MsG0380011927.01.T01:CDS
CAGCAAAAGAAGACAACATC+TGG 0.443589 3:-8069169 MsG0380011927.01.T01:CDS
GTGTTCACAGCTTTCAGCAT+TGG 0.501579 3:-8069223 MsG0380011927.01.T01:CDS
TGCATATCACATGTACAACT+TGG 0.529028 3:-8069284 MsG0380011927.01.T01:CDS
AAGAGGATGGAAGGCGGACA+TGG 0.536915 3:-8069331 MsG0380011927.01.T01:CDS
TTCATTCAACTGGTGTGAAG+AGG 0.561102 3:-8069348 MsG0380011927.01.T01:CDS
ACTCACTAATTTGGAGATGG+AGG 0.570823 3:+8069378 None:intergenic
GCATATACTCTGGCTCGCTA+TGG 0.570951 3:+8069308 None:intergenic
TTCAACTGGTGTGAAGAGGA+TGG 0.571841 3:-8069344 MsG0380011927.01.T01:CDS
TCTACTCACTAATTTGGAGA+TGG 0.576341 3:+8069375 None:intergenic
TGGAAAATGACTGCATACAC+AGG 0.585917 3:+8069190 None:intergenic
ATCTCCAAATTAGTGAGTAG+AGG 0.593200 3:-8069373 MsG0380011927.01.T01:CDS
TTGGACAAGATGAAGTATCA+GGG 0.612137 3:-8069265 MsG0380011927.01.T01:CDS
ACTGGTGTGAAGAGGATGGA+AGG 0.612873 3:-8069340 MsG0380011927.01.T01:CDS
TGATATGCATGCATATACTC+TGG 0.624731 3:+8069298 None:intergenic
ATAACGATGGCATTGAACAA+CGG 0.641834 3:-8069439 None:intergenic
GGTGTGAAGAGGATGGAAGG+CGG 0.703869 3:-8069337 MsG0380011927.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATCCTCTACTCACTAATT+TGG + Chr3:8069232-8069251 None:intergenic 30.0%
AGTAGAGGATTTCATTCAAC+TGG - Chr3:8069240-8069259 MsG0380011927.01.T01:CDS 35.0%
ATCTCCAAATTAGTGAGTAG+AGG - Chr3:8069225-8069244 MsG0380011927.01.T01:CDS 35.0%
TCTACTCACTAATTTGGAGA+TGG + Chr3:8069226-8069245 None:intergenic 35.0%
TGATATGCATGCATATACTC+TGG + Chr3:8069303-8069322 None:intergenic 35.0%
TGCATATCACATGTACAACT+TGG - Chr3:8069314-8069333 MsG0380011927.01.T01:CDS 35.0%
! TTGGACAAGATGAAGTATCA+GGG - Chr3:8069333-8069352 MsG0380011927.01.T01:CDS 35.0%
ACTCACTAATTTGGAGATGG+AGG + Chr3:8069223-8069242 None:intergenic 40.0%
CAGCAAAAGAAGACAACATC+TGG - Chr3:8069429-8069448 MsG0380011927.01.T01:CDS 40.0%
TGGAAAATGACTGCATACAC+AGG + Chr3:8069411-8069430 None:intergenic 40.0%
TTCATTCAACTGGTGTGAAG+AGG - Chr3:8069250-8069269 MsG0380011927.01.T01:CDS 40.0%
! CAGATGTTGTCTTCTTTTGC+TGG + Chr3:8069431-8069450 None:intergenic 40.0%
! CTTGGACAAGATGAAGTATC+AGG - Chr3:8069332-8069351 MsG0380011927.01.T01:CDS 40.0%
GTGTTCACAGCTTTCAGCAT+TGG - Chr3:8069375-8069394 MsG0380011927.01.T01:CDS 45.0%
TTCAACTGGTGTGAAGAGGA+TGG - Chr3:8069254-8069273 MsG0380011927.01.T01:CDS 45.0%
ACTGGTGTGAAGAGGATGGA+AGG - Chr3:8069258-8069277 MsG0380011927.01.T01:CDS 50.0%
! GCATATACTCTGGCTCGCTA+TGG + Chr3:8069293-8069312 None:intergenic 50.0%
AAGAGGATGGAAGGCGGACA+TGG - Chr3:8069267-8069286 MsG0380011927.01.T01:CDS 55.0%
GGTGTGAAGAGGATGGAAGG+CGG - Chr3:8069261-8069280 MsG0380011927.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 8069165 8069455 8069165 ID=MsG0380011927.01;Name=MsG0380011927.01
Chr3 mRNA 8069165 8069455 8069165 ID=MsG0380011927.01.T01;Parent=MsG0380011927.01;Name=MsG0380011927.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|96
Chr3 exon 8069165 8069455 8069165 ID=MsG0380011927.01.T01:exon:3228;Parent=MsG0380011927.01.T01
Chr3 CDS 8069165 8069455 8069165 ID=MsG0380011927.01.T01:cds;Parent=MsG0380011927.01.T01
Gene Sequence

>MsG0380011927.01.T01

ATGGCATTGAACAACGGTCTCAGATCTGCTGCGAAGCTAATTGCTTCTTCAGAATCCTCCATCTCCAAATTAGTGAGTAGAGGATTTCATTCAACTGGTGTGAAGAGGATGGAAGGCGGACATGGCCATAGCGAGCCAGAGTATATGCATGCATATCACATGTACAACTTGGACAAGATGAAGTATCAGGGTTTGAAAATGTCTCTTGCTGTGTTCACAGCTTTCAGCATTGGTGTTGCAGTTCCTGTGTATGCAGTCATTTTCCAGCAAAAGAAGACAACATCTGGCTGA

Protein sequence

>MsG0380011927.01.T01

MALNNGLRSAAKLIASSESSISKLVSRGFHSTGVKRMEGGHGHSEPEYMHAYHMYNLDKMKYQGLKMSLAVFTAFSIGVAVPVYAVIFQQKKTTSG*