Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048764.01 | MsG0580025936.01 | 0.841429 | 4.778525e-58 | 7.019843e-55 |
| MsG0180000152.01 | MsG0580025936.01 | 0.812988 | 3.212579e-51 | 2.072464e-48 |
| MsG0180000470.01 | MsG0580025936.01 | 0.808420 | 3.124578e-50 | 1.785739e-47 |
| MsG0180000550.01 | MsG0580025936.01 | 0.808580 | 2.888604e-50 | 1.657711e-47 |
| MsG0180000828.01 | MsG0580025936.01 | 0.806718 | 7.177985e-50 | 3.924047e-47 |
| MsG0180001141.01 | MsG0580025936.01 | 0.824918 | 6.220199e-54 | 5.586744e-51 |
| MsG0180002482.01 | MsG0580025936.01 | 0.820799 | 5.668366e-53 | 4.530179e-50 |
| MsG0180003854.01 | MsG0580025936.01 | 0.816748 | 4.713296e-52 | 3.368146e-49 |
| MsG0180004886.01 | MsG0580025936.01 | -0.800857 | 1.183737e-48 | 5.570916e-46 |
| MsG0180005371.01 | MsG0580025936.01 | 0.802303 | 5.980973e-49 | 2.919889e-46 |
| MsG0180006220.01 | MsG0580025936.01 | 0.810846 | 9.409797e-51 | 5.734354e-48 |
| MsG0280006411.01 | MsG0580025936.01 | 0.824451 | 8.014286e-54 | 7.100943e-51 |
| MsG0280010551.01 | MsG0580025936.01 | 0.814329 | 1.628677e-51 | 1.089621e-48 |
| MsG0280011156.01 | MsG0580025936.01 | 0.806302 | 8.785732e-50 | 4.752180e-47 |
| MsG0380011661.01 | MsG0580025936.01 | 0.836299 | 1.015174e-56 | 1.274218e-53 |
| MsG0380011895.01 | MsG0580025936.01 | 0.808285 | 3.338886e-50 | 1.901484e-47 |
| MsG0380012566.01 | MsG0580025936.01 | 0.800882 | 1.170132e-48 | 5.510381e-46 |
| MsG0380014770.01 | MsG0580025936.01 | 0.806308 | 8.761046e-50 | 4.739544e-47 |
| MsG0480018603.01 | MsG0580025936.01 | 0.811428 | 7.037142e-51 | 4.354977e-48 |
| MsG0480018883.01 | MsG0580025936.01 | 0.809663 | 1.692740e-50 | 9.998609e-48 |
| MsG0480019163.01 | MsG0580025936.01 | -0.808535 | 2.953179e-50 | 1.692826e-47 |
| MsG0480020136.01 | MsG0580025936.01 | 0.806327 | 8.679977e-50 | 4.698005e-47 |
| MsG0480020521.01 | MsG0580025936.01 | 0.807984 | 3.870232e-50 | 2.186553e-47 |
| MsG0480020839.01 | MsG0580025936.01 | 0.811200 | 7.885587e-51 | 4.850683e-48 |
| MsG0480020874.01 | MsG0580025936.01 | 0.807666 | 4.520595e-50 | 2.532847e-47 |
| MsG0480021274.01 | MsG0580025936.01 | 0.806614 | 7.553422e-50 | 4.118107e-47 |
| MsG0480021448.01 | MsG0580025936.01 | 0.813351 | 2.674568e-51 | 1.742522e-48 |
| MsG0480022052.01 | MsG0580025936.01 | -0.827925 | 1.195183e-54 | 1.170275e-51 |
| MsG0480022222.01 | MsG0580025936.01 | 0.834141 | 3.557289e-56 | 4.183608e-53 |
| MsG0480022768.01 | MsG0580025936.01 | 0.816975 | 4.190791e-52 | 3.013702e-49 |
| MsG0480023174.01 | MsG0580025936.01 | 0.801596 | 8.357477e-49 | 4.006947e-46 |
| MsG0480023814.01 | MsG0580025936.01 | 0.824544 | 7.622782e-54 | 6.772830e-51 |
| MsG0580024165.01 | MsG0580025936.01 | 0.813454 | 2.539333e-51 | 1.659107e-48 |
| MsG0580024166.01 | MsG0580025936.01 | 0.800817 | 1.206065e-48 | 5.669923e-46 |
| MsG0580025936.01 | MsG0580026718.01 | 0.811431 | 7.027039e-51 | 4.349034e-48 |
| MsG0580025936.01 | MsG0580026777.01 | 0.967749 | 1.044218e-127 | 8.131591e-122 |
| MsG0580025936.01 | MsG0580027344.01 | 0.824182 | 9.273397e-54 | 8.154101e-51 |
| MsG0580025936.01 | MsG0680031379.01 | 0.842083 | 3.211333e-58 | 4.814974e-55 |
| MsG0580025936.01 | MsG0680031646.01 | 0.830551 | 2.755676e-55 | 2.912754e-52 |
| MsG0580025936.01 | MsG0680031967.01 | 0.807039 | 6.141948e-50 | 3.385612e-47 |
| MsG0580025936.01 | MsG0680032430.01 | 0.816031 | 6.819813e-52 | 4.778968e-49 |
| MsG0580025936.01 | MsG0680033391.01 | 0.823207 | 1.567949e-53 | 1.341274e-50 |
| MsG0580025936.01 | MsG0680033792.01 | 0.829395 | 5.272379e-55 | 5.389702e-52 |
| MsG0580025936.01 | MsG0680035346.01 | 0.801444 | 8.977721e-49 | 4.288131e-46 |
| MsG0580025936.01 | MsG0680035704.01 | 0.812120 | 4.974297e-51 | 3.135647e-48 |
| MsG0580025936.01 | MsG0780035948.01 | 0.805020 | 1.632814e-49 | 8.546143e-47 |
| MsG0580025936.01 | MsG0780037082.01 | 0.803143 | 4.012021e-49 | 2.000886e-46 |
| MsG0580025936.01 | MsG0780040596.01 | 0.800831 | 1.198529e-48 | 5.636542e-46 |
| MsG0580025936.01 | MsG0780041456.01 | 0.806364 | 8.526617e-50 | 4.619181e-47 |
| MsG0580025936.01 | MsG0880042055.01 | 0.802091 | 6.611804e-49 | 3.210319e-46 |
| MsG0580025936.01 | MsG0880042294.01 | 0.813984 | 1.940467e-51 | 1.286042e-48 |
| MsG0580025936.01 | MsG0880044574.01 | 0.815223 | 1.031779e-51 | 7.071402e-49 |
| MsG0580025936.01 | MsG0880045362.01 | 0.807932 | 3.968797e-50 | 2.239141e-47 |
| MsG0580025936.01 | MsG0880045653.01 | 0.815828 | 7.569616e-52 | 5.274731e-49 |
| MsG0580025936.01 | MsG0880046285.01 | 0.812405 | 4.310989e-51 | 2.738736e-48 |
| MsG0580025936.01 | MsG0880047116.01 | 0.803363 | 3.613723e-49 | 1.812360e-46 |
| MsG0580025936.01 | MsG0880047191.01 | 0.803653 | 3.145466e-49 | 1.589402e-46 |
| MsG0580025936.01 | MsG0880047425.01 | 0.801717 | 7.891060e-49 | 3.795103e-46 |
| MsG0580025936.01 | MsG0880047608.01 | 0.801330 | 9.476188e-49 | 4.513165e-46 |
| MsG0580025936.01 | MsG0880047689.01 | -0.811042 | 8.533926e-51 | 5.227809e-48 |
| MsG0380015164.01 | MsG0580025936.01 | 0.801140 | 1.036305e-48 | 4.912147e-46 |
| MsG0380015466.01 | MsG0580025936.01 | 0.811774 | 5.917161e-51 | 3.695375e-48 |
| MsG0380016004.01 | MsG0580025936.01 | 0.815020 | 1.145009e-51 | 7.804562e-49 |
| MsG0380016064.01 | MsG0580025936.01 | 0.805883 | 1.076576e-49 | 5.761263e-47 |
| MsG0380016581.01 | MsG0580025936.01 | 0.800354 | 1.499304e-48 | 6.966413e-46 |
| MsG0380016910.01 | MsG0580025936.01 | 0.817296 | 3.550022e-52 | 2.575183e-49 |
| MsG0380017266.01 | MsG0580025936.01 | -0.800714 | 1.266186e-48 | 5.936875e-46 |
| MsG0380017680.01 | MsG0580025936.01 | 0.810476 | 1.131301e-50 | 6.826116e-48 |
| MsG0380017746.01 | MsG0580025936.01 | 0.805046 | 1.612984e-49 | 8.447920e-47 |
| MsG0380017998.01 | MsG0580025936.01 | 0.803492 | 3.397784e-49 | 1.709852e-46 |
| MsG0480018163.01 | MsG0580025936.01 | 0.822477 | 2.319968e-53 | 1.944006e-50 |
| MsG0480018331.01 | MsG0580025936.01 | 0.802266 | 6.086093e-49 | 2.968271e-46 |
| MsG0280006456.01 | MsG0580025936.01 | 0.814815 | 1.271215e-51 | 8.616856e-49 |
| MsG0280007222.01 | MsG0580025936.01 | 0.800258 | 1.567890e-48 | 7.267301e-46 |
| MsG0280009140.01 | MsG0580025936.01 | 0.800490 | 1.406318e-48 | 6.556880e-46 |
| MsG0280010443.01 | MsG0580025936.01 | 0.826305 | 2.917763e-54 | 2.725973e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAGCGCATGGAAGCTTT+TGG | 0.283497 | 5:+27489197 | MsG0580025936.01.T01:CDS |
| AATTATGGGTAGATGTTGTT+GGG | 0.294283 | 5:+27488941 | None:intergenic |
| CGAGGTAATTGTTATGGTAC+TGG | 0.324310 | 5:+27488972 | MsG0580025936.01.T01:CDS |
| GATCAGCTTCAGCAGCTCTT+TGG | 0.342233 | 5:-27489124 | None:intergenic |
| CAATTATGGGTAGATGTTGT+TGG | 0.353756 | 5:+27488940 | None:intergenic |
| TTGTCTCGAGGTAATTGTTA+TGG | 0.375766 | 5:+27488966 | MsG0580025936.01.T01:CDS |
| GGCTGCTGCAGCACGTGCTG+AGG | 0.435827 | 5:+27489088 | MsG0580025936.01.T01:CDS |
| GAGGTAATTGTTATGGTACT+GGG | 0.483970 | 5:+27488973 | MsG0580025936.01.T01:CDS |
| GGTGATAGAAATCTTTCGTC+AGG | 0.501028 | 5:+27489064 | MsG0580025936.01.T01:CDS |
| GAATCAATTCTCTACTGCTA+TGG | 0.520159 | 5:+27489166 | MsG0580025936.01.T01:CDS |
| TGAGTCAGATGACTTACCCC+TGG | 0.525124 | 5:-27489017 | None:intergenic |
| ATTATGGGTAGATGTTGTTG+GGG | 0.545796 | 5:+27488942 | None:intergenic |
| TGTTGTTGGGGTTTGTCTCG+AGG | 0.554513 | 5:+27488954 | MsG0580025936.01.T01:CDS |
| TTATCTTTCAACATTAGACC+AGG | 0.567117 | 5:+27488999 | MsG0580025936.01.T01:CDS |
| ATGTCGTCCTCCTGAAAATG+AGG | 0.579452 | 5:+27489043 | MsG0580025936.01.T01:CDS |
| GGAAGAACTCAGGAAGCGCA+TGG | 0.581997 | 5:+27489187 | MsG0580025936.01.T01:CDS |
| ATCTTTCAACATTAGACCAG+GGG | 0.586886 | 5:+27489001 | MsG0580025936.01.T01:CDS |
| CTACTGCTATGGAAGAACTC+AGG | 0.602511 | 5:+27489177 | MsG0580025936.01.T01:CDS |
| TATCTTTCAACATTAGACCA+GGG | 0.629793 | 5:+27489000 | MsG0580025936.01.T01:CDS |
| AGGTAATTGTTATGGTACTG+GGG | 0.660624 | 5:+27488974 | MsG0580025936.01.T01:CDS |
| GATAGAAATCTTTCGTCAGG+AGG | 0.670758 | 5:+27489067 | MsG0580025936.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATCTTTCAACATTAGACCA+GGG | + | Chr5:27489000-27489019 | MsG0580025936.01.T01:CDS | 30.0% | |
| TTATCTTTCAACATTAGACC+AGG | + | Chr5:27488999-27489018 | MsG0580025936.01.T01:CDS | 30.0% | |
| ! | ATTTCTATCACCTCATTTTC+AGG | - | Chr5:27489056-27489075 | None:intergenic | 30.0% |
| AGGTAATTGTTATGGTACTG+GGG | + | Chr5:27488974-27488993 | MsG0580025936.01.T01:CDS | 35.0% | |
| ATCTTTCAACATTAGACCAG+GGG | + | Chr5:27489001-27489020 | MsG0580025936.01.T01:CDS | 35.0% | |
| GAATCAATTCTCTACTGCTA+TGG | + | Chr5:27489166-27489185 | MsG0580025936.01.T01:CDS | 35.0% | |
| GAGGTAATTGTTATGGTACT+GGG | + | Chr5:27488973-27488992 | MsG0580025936.01.T01:CDS | 35.0% | |
| TTGTCTCGAGGTAATTGTTA+TGG | + | Chr5:27488966-27488985 | MsG0580025936.01.T01:CDS | 35.0% | |
| CGAGGTAATTGTTATGGTAC+TGG | + | Chr5:27488972-27488991 | MsG0580025936.01.T01:CDS | 40.0% | |
| GATAGAAATCTTTCGTCAGG+AGG | + | Chr5:27489067-27489086 | MsG0580025936.01.T01:CDS | 40.0% | |
| GGTGATAGAAATCTTTCGTC+AGG | + | Chr5:27489064-27489083 | MsG0580025936.01.T01:CDS | 40.0% | |
| !! | TCTATCACCTCATTTTCAGG+AGG | - | Chr5:27489053-27489072 | None:intergenic | 40.0% |
| ATGTCGTCCTCCTGAAAATG+AGG | + | Chr5:27489043-27489062 | MsG0580025936.01.T01:CDS | 45.0% | |
| CTACTGCTATGGAAGAACTC+AGG | + | Chr5:27489177-27489196 | MsG0580025936.01.T01:CDS | 45.0% | |
| GATCAGCTTCAGCAGCTCTT+TGG | - | Chr5:27489127-27489146 | None:intergenic | 50.0% | |
| TGAGTCAGATGACTTACCCC+TGG | - | Chr5:27489020-27489039 | None:intergenic | 50.0% | |
| TGTTGTTGGGGTTTGTCTCG+AGG | + | Chr5:27488954-27488973 | MsG0580025936.01.T01:CDS | 50.0% | |
| !! | AGGAAGCGCATGGAAGCTTT+TGG | + | Chr5:27489197-27489216 | MsG0580025936.01.T01:CDS | 50.0% |
| ! | GGAAGAACTCAGGAAGCGCA+TGG | + | Chr5:27489187-27489206 | MsG0580025936.01.T01:CDS | 55.0% |
| GGCTGCTGCAGCACGTGCTG+AGG | + | Chr5:27489088-27489107 | MsG0580025936.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 27488945 | 27489259 | 27488945 | ID=MsG0580025936.01;Name=MsG0580025936.01 |
| Chr5 | mRNA | 27488945 | 27489259 | 27488945 | ID=MsG0580025936.01.T01;Parent=MsG0580025936.01;Name=MsG0580025936.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|104 |
| Chr5 | exon | 27488945 | 27489259 | 27488945 | ID=MsG0580025936.01.T01:exon:29213;Parent=MsG0580025936.01.T01 |
| Chr5 | CDS | 27488945 | 27489259 | 27488945 | ID=MsG0580025936.01.T01:cds;Parent=MsG0580025936.01.T01 |
| Gene Sequence |
| Protein sequence |
