Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025628.01.T01 | XP_003613025.1 | 91.156 | 147 | 13 | 0 | 1 | 147 | 1 | 147 | 3.34E-90 | 280 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025628.01.T01 | U3U992 | 46.939 | 147 | 66 | 4 | 10 | 147 | 9 | 152 | 4.55E-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025628.01.T01 | G7JWC2 | 91.156 | 147 | 13 | 0 | 1 | 147 | 1 | 147 | 1.60e-90 | 280 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048849.01 | MsG0580025628.01 | 0.804695 | 1.909323e-49 | 9.910194e-47 |
| MsG0080049002.01 | MsG0580025628.01 | 0.804964 | 1.677863e-49 | 8.769624e-47 |
| MsG0080049068.01 | MsG0580025628.01 | 0.802196 | 6.291515e-49 | 3.062855e-46 |
| MsG0180000599.01 | MsG0580025628.01 | 0.802149 | 6.432771e-49 | 3.127992e-46 |
| MsG0180001610.01 | MsG0580025628.01 | 0.810372 | 1.191151e-50 | 7.167422e-48 |
| MsG0180001704.01 | MsG0580025628.01 | 0.808409 | 3.142033e-50 | 1.795177e-47 |
| MsG0180002024.01 | MsG0580025628.01 | 0.819177 | 1.331515e-52 | 1.017232e-49 |
| MsG0180002399.01 | MsG0580025628.01 | 0.814944 | 1.190182e-51 | 8.095480e-49 |
| MsG0180002700.01 | MsG0580025628.01 | 0.828140 | 1.060515e-54 | 1.045043e-51 |
| MsG0180004246.01 | MsG0580025628.01 | 0.839687 | 1.365269e-57 | 1.899739e-54 |
| MsG0180004428.01 | MsG0580025628.01 | 0.807118 | 5.909074e-50 | 3.263859e-47 |
| MsG0180004448.01 | MsG0580025628.01 | 0.813416 | 2.588674e-51 | 1.689516e-48 |
| MsG0180006136.01 | MsG0580025628.01 | 0.805102 | 1.569890e-49 | 8.234113e-47 |
| MsG0280011255.01 | MsG0580025628.01 | 0.855205 | 7.375641e-62 | 1.689459e-58 |
| MsG0380012011.01 | MsG0580025628.01 | 0.816204 | 6.237498e-52 | 4.391786e-49 |
| MsG0380012567.01 | MsG0580025628.01 | 0.803336 | 3.659809e-49 | 1.834278e-46 |
| MsG0380014386.01 | MsG0580025628.01 | 0.803759 | 2.990068e-49 | 1.515068e-46 |
| MsG0480018673.01 | MsG0580025628.01 | 0.821588 | 3.728662e-53 | 3.046021e-50 |
| MsG0480020690.01 | MsG0580025628.01 | 0.811174 | 7.986999e-51 | 4.909657e-48 |
| MsG0480021248.01 | MsG0580025628.01 | 0.823033 | 1.722118e-53 | 1.466029e-50 |
| MsG0480021377.01 | MsG0580025628.01 | 0.803765 | 2.982586e-49 | 1.511466e-46 |
| MsG0480022994.01 | MsG0580025628.01 | 0.806753 | 7.059483e-50 | 3.862666e-47 |
| MsG0480023150.01 | MsG0580025628.01 | 0.805205 | 1.493634e-49 | 7.855038e-47 |
| MsG0580024340.01 | MsG0580025628.01 | 0.800936 | 1.140341e-48 | 5.377469e-46 |
| MsG0580024759.01 | MsG0580025628.01 | 0.811063 | 8.442565e-51 | 5.174686e-48 |
| MsG0580025059.01 | MsG0580025628.01 | 0.832746 | 7.928909e-56 | 8.944150e-53 |
| MsG0580025366.01 | MsG0580025628.01 | 0.832358 | 9.896885e-56 | 1.103551e-52 |
| MsG0580025626.01 | MsG0580025628.01 | 0.808432 | 3.106778e-50 | 1.776157e-47 |
| MsG0580025627.01 | MsG0580025628.01 | 0.826506 | 2.613141e-54 | 2.455387e-51 |
| MsG0580025628.01 | MsG0580025629.01 | 0.835258 | 1.862827e-56 | 2.265906e-53 |
| MsG0580025628.01 | MsG0580027394.01 | 0.843961 | 1.014973e-58 | 1.614383e-55 |
| MsG0580025628.01 | MsG0580028419.01 | 0.830779 | 2.422842e-55 | 2.578418e-52 |
| MsG0580025628.01 | MsG0580029734.01 | 0.808029 | 3.785659e-50 | 2.141329e-47 |
| MsG0580025628.01 | MsG0680030514.01 | 0.814078 | 1.850559e-51 | 1.229622e-48 |
| MsG0580025628.01 | MsG0680030680.01 | 0.801919 | 7.173827e-49 | 3.467918e-46 |
| MsG0580025628.01 | MsG0680030858.01 | 0.834636 | 2.673393e-56 | 3.191329e-53 |
| MsG0580025628.01 | MsG0680031146.01 | 0.812810 | 3.515408e-51 | 2.256958e-48 |
| MsG0580025628.01 | MsG0680032830.01 | 0.801162 | 1.025356e-48 | 4.862919e-46 |
| MsG0580025628.01 | MsG0680033377.01 | 0.815689 | 8.129552e-52 | 5.643779e-49 |
| MsG0580025628.01 | MsG0680034766.01 | 0.822939 | 1.811459e-53 | 1.537938e-50 |
| MsG0580025628.01 | MsG0680035859.01 | 0.804441 | 2.157229e-49 | 1.112330e-46 |
| MsG0580025628.01 | MsG0780036517.01 | 0.812982 | 3.223335e-51 | 2.079002e-48 |
| MsG0580025628.01 | MsG0780036792.01 | 0.817326 | 3.494462e-52 | 2.537043e-49 |
| MsG0580025628.01 | MsG0780037363.01 | 0.824509 | 7.767789e-54 | 6.894523e-51 |
| MsG0580025628.01 | MsG0780041262.01 | 0.824051 | 9.949845e-54 | 8.716163e-51 |
| MsG0580025628.01 | MsG0880041852.01 | 0.804030 | 2.626506e-49 | 1.340137e-46 |
| MsG0580025628.01 | MsG0880042086.01 | 0.830688 | 2.551225e-55 | 2.707801e-52 |
| MsG0580025628.01 | MsG0880044036.01 | 0.815858 | 7.452788e-52 | 5.197573e-49 |
| MsG0580025628.01 | MsG0880044487.01 | 0.810961 | 8.882140e-51 | 5.429912e-48 |
| MsG0580025628.01 | MsG0880044985.01 | 0.812005 | 5.269985e-51 | 3.311759e-48 |
| MsG0580025628.01 | MsG0880045722.01 | 0.817236 | 3.661618e-52 | 2.651831e-49 |
| MsG0580025628.01 | MsG0880046765.01 | 0.809822 | 1.564740e-50 | 9.281171e-48 |
| MsG0580025628.01 | MsG0880047394.01 | 0.801823 | 7.507225e-49 | 3.620314e-46 |
| MsG0580025628.01 | MsG0880047481.01 | -0.816349 | 5.788356e-52 | 4.091778e-49 |
| MsG0580025628.01 | MsG0880047657.01 | 0.800379 | 1.481814e-48 | 6.889597e-46 |
| MsG0380015372.01 | MsG0580025628.01 | 0.829032 | 6.456951e-55 | 6.529645e-52 |
| MsG0380015784.01 | MsG0580025628.01 | 0.823713 | 1.194269e-53 | 1.036497e-50 |
| MsG0380015896.01 | MsG0580025628.01 | 0.805927 | 1.053616e-49 | 5.644762e-47 |
| MsG0380016540.01 | MsG0580025628.01 | 0.803939 | 2.744064e-49 | 1.396914e-46 |
| MsG0380017931.01 | MsG0580025628.01 | 0.807943 | 3.948806e-50 | 2.228507e-47 |
| MsG0280008929.01 | MsG0580025628.01 | 0.806065 | 9.858814e-50 | 5.300513e-47 |
| MsG0280009256.01 | MsG0580025628.01 | 0.821563 | 3.778364e-53 | 3.084733e-50 |
| MsG0280009507.01 | MsG0580025628.01 | 0.821440 | 4.033000e-53 | 3.281200e-50 |
| MsG0280009755.01 | MsG0580025628.01 | 0.816148 | 6.421265e-52 | 4.514078e-49 |
| MsG0280009756.01 | MsG0580025628.01 | 0.838710 | 2.446286e-57 | 3.304165e-54 |
| MsG0280010353.01 | MsG0580025628.01 | 0.834276 | 3.290617e-56 | 3.886345e-53 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025628.01.T01 | MTR_5g031830 | 91.156 | 147 | 13 | 0 | 1 | 147 | 1 | 147 | 4.05e-94 | 280 |
| MsG0580025628.01.T01 | MTR_5g031860 | 89.116 | 147 | 16 | 0 | 1 | 147 | 1 | 147 | 9.33e-93 | 276 |
| MsG0580025628.01.T01 | MTR_5g031840 | 82.014 | 139 | 24 | 1 | 10 | 147 | 3 | 141 | 1.42e-78 | 240 |
| MsG0580025628.01.T01 | MTR_3g479490 | 38.776 | 147 | 71 | 6 | 12 | 147 | 6 | 144 | 4.25e-18 | 80.1 |
| MsG0580025628.01.T01 | MTR_3g479620 | 36.301 | 146 | 82 | 6 | 9 | 147 | 3 | 144 | 6.46e-18 | 76.3 |
| MsG0580025628.01.T01 | MTR_5g464760 | 34.615 | 156 | 88 | 5 | 1 | 147 | 1 | 151 | 2.19e-17 | 77.8 |
| MsG0580025628.01.T01 | MTR_3g479520 | 34.932 | 146 | 86 | 4 | 8 | 147 | 11 | 153 | 1.87e-16 | 75.5 |
| MsG0580025628.01.T01 | MTR_4g094220 | 33.803 | 142 | 77 | 6 | 7 | 137 | 2 | 137 | 1.43e-15 | 72.8 |
| MsG0580025628.01.T01 | MTR_3g079480 | 32.903 | 155 | 71 | 5 | 11 | 147 | 20 | 159 | 8.04e-15 | 70.5 |
| MsG0580025628.01.T01 | MTR_0036s0220 | 33.775 | 151 | 82 | 7 | 8 | 147 | 8 | 151 | 1.05e-14 | 70.5 |
| MsG0580025628.01.T01 | MTR_3g479470 | 34.868 | 152 | 72 | 7 | 11 | 147 | 12 | 151 | 1.92e-13 | 66.6 |
| MsG0580025628.01.T01 | MTR_2g008210 | 34.965 | 143 | 81 | 6 | 11 | 147 | 10 | 146 | 3.42e-13 | 65.9 |
| MsG0580025628.01.T01 | MTR_5g464810 | 33.758 | 157 | 74 | 7 | 8 | 147 | 8 | 151 | 3.87e-13 | 65.9 |
| MsG0580025628.01.T01 | MTR_5g064240 | 33.758 | 157 | 74 | 7 | 8 | 147 | 8 | 151 | 3.87e-13 | 65.9 |
| MsG0580025628.01.T01 | MTR_7g013220 | 32.667 | 150 | 83 | 5 | 11 | 147 | 4 | 148 | 7.85e-13 | 65.1 |
| MsG0580025628.01.T01 | MTR_6g014220 | 43.284 | 67 | 36 | 1 | 11 | 77 | 9 | 73 | 5.82e-12 | 62.4 |
| MsG0580025628.01.T01 | MTR_6g013440 | 40.845 | 71 | 40 | 1 | 7 | 77 | 6 | 74 | 7.36e-12 | 62.0 |
| MsG0580025628.01.T01 | MTR_6g014020 | 40.000 | 70 | 40 | 1 | 8 | 77 | 6 | 73 | 1.16e-11 | 61.6 |
| MsG0580025628.01.T01 | MTR_6g014270 | 54.348 | 46 | 21 | 0 | 9 | 54 | 4 | 49 | 1.32e-11 | 61.2 |
| MsG0580025628.01.T01 | MTR_0475s0020 | 54.545 | 44 | 20 | 0 | 11 | 54 | 9 | 52 | 1.52e-11 | 60.1 |
| MsG0580025628.01.T01 | MTR_3g008010 | 54.348 | 46 | 21 | 0 | 9 | 54 | 7 | 52 | 2.18e-11 | 60.8 |
| MsG0580025628.01.T01 | MTR_8g059425 | 55.319 | 47 | 21 | 0 | 8 | 54 | 6 | 52 | 2.29e-11 | 60.8 |
| MsG0580025628.01.T01 | MTR_0288s0020 | 54.545 | 44 | 20 | 0 | 11 | 54 | 9 | 52 | 2.55e-11 | 60.5 |
| MsG0580025628.01.T01 | MTR_6g014040 | 31.333 | 150 | 86 | 6 | 11 | 147 | 9 | 154 | 2.73e-11 | 60.5 |
| MsG0580025628.01.T01 | MTR_6g014290 | 54.545 | 44 | 20 | 0 | 11 | 54 | 9 | 52 | 3.60e-11 | 60.1 |
| MsG0580025628.01.T01 | MTR_6g014170 | 54.545 | 44 | 20 | 0 | 11 | 54 | 9 | 52 | 6.44e-11 | 59.3 |
| MsG0580025628.01.T01 | MTR_6g014187 | 39.437 | 71 | 41 | 1 | 7 | 77 | 5 | 73 | 7.87e-11 | 59.3 |
| MsG0580025628.01.T01 | MTR_7g013200 | 32.639 | 144 | 80 | 5 | 11 | 147 | 4 | 137 | 9.23e-11 | 58.9 |
| MsG0580025628.01.T01 | MTR_6g014186 | 38.806 | 67 | 39 | 1 | 11 | 77 | 6 | 70 | 9.98e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025628.01.T01 | AT1G22380 | 35.570 | 149 | 81 | 6 | 8 | 147 | 9 | 151 | 9.32e-20 | 84.7 |
| MsG0580025628.01.T01 | AT1G22370 | 36.986 | 146 | 77 | 6 | 11 | 147 | 12 | 151 | 4.82e-17 | 77.0 |
| MsG0580025628.01.T01 | AT1G22340 | 34.014 | 147 | 80 | 6 | 11 | 147 | 12 | 151 | 1.53e-15 | 72.8 |
| MsG0580025628.01.T01 | AT3G02100 | 33.333 | 153 | 85 | 5 | 6 | 147 | 7 | 153 | 6.54e-15 | 70.9 |
| MsG0580025628.01.T01 | AT1G22360 | 31.333 | 150 | 78 | 4 | 12 | 147 | 10 | 148 | 2.28e-14 | 69.3 |
| MsG0580025628.01.T01 | AT1G22360 | 31.333 | 150 | 78 | 4 | 12 | 147 | 10 | 148 | 2.59e-14 | 69.3 |
| MsG0580025628.01.T01 | AT1G22400 | 27.815 | 151 | 84 | 4 | 11 | 147 | 12 | 151 | 1.32e-13 | 67.4 |
| MsG0580025628.01.T01 | AT1G78270 | 44.776 | 67 | 35 | 2 | 11 | 77 | 12 | 76 | 5.62e-12 | 62.4 |
Find 22 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATAAGTTGCATTATTT+TGG | 0.129986 | 5:+21470020 | MsG0580025628.01.T01:CDS |
| ACTGATTTGGCTGCTGAGTT+TGG | 0.266869 | 5:+21470066 | MsG0580025628.01.T01:CDS |
| GAGGAGAAGCATCCTGGATT+TGG | 0.312050 | 5:+21469922 | MsG0580025628.01.T01:CDS |
| CTGATTTGGCTGCTGAGTTT+GGG | 0.334780 | 5:+21470067 | MsG0580025628.01.T01:CDS |
| TGGTGATCTTGCTACTGATT+TGG | 0.352180 | 5:+21470053 | MsG0580025628.01.T01:CDS |
| CACAGTGAGGAGAAGCATCC+TGG | 0.364154 | 5:+21469916 | MsG0580025628.01.T01:CDS |
| ATGGTCTTGAATTGTAGCTT+AGG | 0.425350 | 5:-21469883 | None:intergenic |
| AGCTCAGCTAGCTTTAGCAT+TGG | 0.453685 | 5:-21469757 | None:intergenic |
| AGCATCCTGGATTTGGAGAA+AGG | 0.475413 | 5:+21469929 | MsG0580025628.01.T01:CDS |
| GGATTTACATGGCCTTGTGC+TGG | 0.486767 | 5:-21469736 | None:intergenic |
| TGGTCTTGAATTGTAGCTTA+GGG | 0.493157 | 5:-21469882 | None:intergenic |
| GCTTTAGCATTGGATTTACA+TGG | 0.505828 | 5:-21469747 | None:intergenic |
| GCTATCCTTTCTCCAAATCC+AGG | 0.521331 | 5:-21469934 | None:intergenic |
| ACATGGCCTTGTGCTGGACA+TGG | 0.534043 | 5:-21469730 | None:intergenic |
| TGAAACTAATGAGACGGTTG+TGG | 0.551286 | 5:-21469828 | None:intergenic |
| CATGGGAAGATTAGCAAATG+TGG | 0.557319 | 5:-21469712 | None:intergenic |
| CTCACTGTGAAAATCAGAGA+TGG | 0.579959 | 5:-21469902 | None:intergenic |
| CATGGCCTTGTGCTGGACAT+GGG | 0.589394 | 5:-21469729 | None:intergenic |
| ACAATAATATCTTTCAACAA+AGG | 0.592002 | 5:-21469991 | None:intergenic |
| TATCATTGAAACTAATGAGA+CGG | 0.592738 | 5:-21469834 | None:intergenic |
| ATCTTCCCATGTCCAGCACA+AGG | 0.604045 | 5:+21469724 | MsG0580025628.01.T01:CDS |
| TGTGGATGTACTCAGTGTTG+AGG | 0.692218 | 5:-21469810 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAATATCTTTCAACAA+AGG | - | Chr5:21469994-21470013 | None:intergenic | 20.0% |
| !!! | ATTATTTTGGATGGAATATT+TGG | + | Chr5:21470033-21470052 | MsG0580025628.01.T01:CDS | 20.0% |
| !!! | GAAAATAAGTTGCATTATTT+TGG | + | Chr5:21470020-21470039 | MsG0580025628.01.T01:CDS | 20.0% |
| ! | TATCATTGAAACTAATGAGA+CGG | - | Chr5:21469837-21469856 | None:intergenic | 25.0% |
| !! | ATTTTATCACTGAGTTTGTA+TGG | + | Chr5:21469964-21469983 | MsG0580025628.01.T01:CDS | 25.0% |
| !!! | ATAAGTTGCATTATTTTGGA+TGG | + | Chr5:21470024-21470043 | MsG0580025628.01.T01:CDS | 25.0% |
| ! | ATGGTCTTGAATTGTAGCTT+AGG | - | Chr5:21469886-21469905 | None:intergenic | 35.0% |
| ! | GCTTTAGCATTGGATTTACA+TGG | - | Chr5:21469750-21469769 | None:intergenic | 35.0% |
| ! | TGGTCTTGAATTGTAGCTTA+GGG | - | Chr5:21469885-21469904 | None:intergenic | 35.0% |
| ! | TGTGGAGAAGATTTTTCCTT+AGG | - | Chr5:21469697-21469716 | None:intergenic | 35.0% |
| CATGGGAAGATTAGCAAATG+TGG | - | Chr5:21469715-21469734 | None:intergenic | 40.0% | |
| CTCACTGTGAAAATCAGAGA+TGG | - | Chr5:21469905-21469924 | None:intergenic | 40.0% | |
| TGAAACTAATGAGACGGTTG+TGG | - | Chr5:21469831-21469850 | None:intergenic | 40.0% | |
| ! | CATCTCTGATTTTCACAGTG+AGG | + | Chr5:21469903-21469922 | MsG0580025628.01.T01:CDS | 40.0% |
| ! | TGGTGATCTTGCTACTGATT+TGG | + | Chr5:21470053-21470072 | MsG0580025628.01.T01:CDS | 40.0% |
| AGCATCCTGGATTTGGAGAA+AGG | + | Chr5:21469929-21469948 | MsG0580025628.01.T01:CDS | 45.0% | |
| GCTATCCTTTCTCCAAATCC+AGG | - | Chr5:21469937-21469956 | None:intergenic | 45.0% | |
| TGTGGATGTACTCAGTGTTG+AGG | - | Chr5:21469813-21469832 | None:intergenic | 45.0% | |
| !! | ACTGATTTGGCTGCTGAGTT+TGG | + | Chr5:21470066-21470085 | MsG0580025628.01.T01:CDS | 45.0% |
| !! | AGCTCAGCTAGCTTTAGCAT+TGG | - | Chr5:21469760-21469779 | None:intergenic | 45.0% |
| !! | CTGATTTGGCTGCTGAGTTT+GGG | + | Chr5:21470067-21470086 | MsG0580025628.01.T01:CDS | 45.0% |
| ATCTTCCCATGTCCAGCACA+AGG | + | Chr5:21469724-21469743 | MsG0580025628.01.T01:CDS | 50.0% | |
| !! | GAGGAGAAGCATCCTGGATT+TGG | + | Chr5:21469922-21469941 | MsG0580025628.01.T01:CDS | 50.0% |
| !! | GGATTTACATGGCCTTGTGC+TGG | - | Chr5:21469739-21469758 | None:intergenic | 50.0% |
| !! | ACATGGCCTTGTGCTGGACA+TGG | - | Chr5:21469733-21469752 | None:intergenic | 55.0% |
| !! | CACAGTGAGGAGAAGCATCC+TGG | + | Chr5:21469916-21469935 | MsG0580025628.01.T01:CDS | 55.0% |
| !! | CATGGCCTTGTGCTGGACAT+GGG | - | Chr5:21469732-21469751 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 21469682 | 21470125 | 21469682 | ID=MsG0580025628.01;Name=MsG0580025628.01 |
| Chr5 | mRNA | 21469682 | 21470125 | 21469682 | ID=MsG0580025628.01.T01;Parent=MsG0580025628.01;Name=MsG0580025628.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|147 |
| Chr5 | exon | 21469682 | 21470125 | 21469682 | ID=MsG0580025628.01.T01:exon:23667;Parent=MsG0580025628.01.T01 |
| Chr5 | CDS | 21469682 | 21470125 | 21469682 | ID=MsG0580025628.01.T01:cds;Parent=MsG0580025628.01.T01 |
| Gene Sequence |
| Protein sequence |
