AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036959.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036959.01.T01 MTR_7g024500 89.474 133 14 0 1 133 118 250 5.90e-84 251
MsG0780036959.01.T01 MTR_7g024520 85.714 133 19 0 1 133 118 250 3.91e-81 243
MsG0780036959.01.T01 MTR_4g088340 67.910 134 38 1 5 133 100 233 2.99e-59 187
MsG0780036959.01.T01 MTR_4g088345 60.784 153 55 1 1 148 119 271 9.62e-59 186
MsG0780036959.01.T01 MTR_7g014570 58.088 136 53 1 2 133 118 253 5.20e-51 166
MsG0780036959.01.T01 MTR_7g014510 53.571 140 61 1 2 137 123 262 1.35e-48 160
MsG0780036959.01.T01 MTR_1g076940 50.327 153 71 2 1 148 125 277 3.42e-47 157
MsG0780036959.01.T01 MTR_1g076930 48.201 139 67 2 1 134 100 238 7.13e-43 142
MsG0780036959.01.T01 MTR_5g074600 48.905 137 65 2 2 133 122 258 1.10e-40 140
MsG0780036959.01.T01 MTR_4g033015 44.311 167 86 3 2 161 125 291 2.42e-40 139
MsG0780036959.01.T01 MTR_4g079160 46.763 139 68 2 1 133 117 255 2.96e-40 139
MsG0780036959.01.T01 MTR_4g033150 50.365 137 61 3 4 133 127 263 3.30e-40 139
MsG0780036959.01.T01 MTR_5g074680 49.630 135 63 2 4 133 125 259 9.34e-40 137
MsG0780036959.01.T01 MTR_4g033200 43.713 167 87 3 2 161 125 291 1.49e-39 137
MsG0780036959.01.T01 MTR_5g074580 48.148 135 65 2 4 133 125 259 3.46e-39 136
MsG0780036959.01.T01 MTR_4g088200 51.825 137 59 3 4 133 125 261 6.53e-39 135
MsG0780036959.01.T01 MTR_4g033085 49.640 139 63 3 2 133 119 257 1.80e-38 134
MsG0780036959.01.T01 MTR_4g033170 49.640 139 63 3 2 133 119 257 2.00e-38 134
MsG0780036959.01.T01 MTR_5g074540 45.255 137 70 2 2 133 104 240 9.99e-37 129
MsG0780036959.01.T01 MTR_4g088190 54.015 137 56 3 4 133 128 264 2.99e-35 126
MsG0780036959.01.T01 MTR_0794s0020 46.341 123 59 3 4 119 127 249 9.06e-32 114
MsG0780036959.01.T01 MTR_5g074610 39.735 151 60 2 2 148 118 241 3.12e-30 112
MsG0780036959.01.T01 MTR_3g083620 35.762 151 92 2 3 148 126 276 2.11e-25 100
MsG0780036959.01.T01 MTR_7g011900 39.831 118 71 0 31 148 170 287 1.84e-23 94.7
MsG0780036959.01.T01 MTR_7g012020 37.963 108 67 0 29 136 167 274 4.78e-23 92.8
MsG0780036959.01.T01 MTR_3g092900 40.000 120 72 0 29 148 161 280 1.55e-22 92.4
MsG0780036959.01.T01 MTR_7g012090 32.117 137 89 1 2 134 142 278 4.11e-22 91.3
MsG0780036959.01.T01 MTR_7g012020 37.736 106 66 0 29 134 167 272 6.34e-22 90.5
MsG0780036959.01.T01 MTR_2g055940 36.667 120 76 0 29 148 161 280 7.81e-22 90.5
MsG0780036959.01.T01 MTR_1g096310 33.108 148 95 1 5 148 142 289 1.03e-21 90.1
MsG0780036959.01.T01 MTR_7g011990 40.187 107 58 2 31 134 167 270 1.14e-21 89.7
MsG0780036959.01.T01 MTR_8g068620 36.697 109 69 0 25 133 2 110 1.70e-21 84.3
MsG0780036959.01.T01 MTR_7g012070 31.387 137 90 1 2 134 142 278 2.56e-21 89.0
MsG0780036959.01.T01 MTR_1g036460 48.193 83 43 0 66 148 204 286 1.09e-20 87.4
MsG0780036959.01.T01 MTR_1g036490 36.800 125 79 0 24 148 162 286 1.40e-20 87.0
MsG0780036959.01.T01 MTR_5g098170 33.775 151 97 1 1 148 130 280 1.58e-20 86.7
MsG0780036959.01.T01 MTR_1g036510 31.293 147 90 1 2 148 165 300 4.44e-20 85.9
MsG0780036959.01.T01 MTR_7g012040 33.962 106 70 0 29 134 166 271 6.62e-20 85.1
MsG0780036959.01.T01 MTR_8g068600 36.697 109 69 0 25 133 145 253 1.65e-19 82.8
MsG0780036959.01.T01 MTR_0591s0010 36.697 109 69 0 25 133 171 279 1.76e-19 84.0
MsG0780036959.01.T01 MTR_1g036510 32.331 133 79 1 2 134 165 286 2.94e-19 82.4
MsG0780036959.01.T01 MTR_4g038440 43.434 99 55 1 37 134 173 271 5.10e-19 81.6
MsG0780036959.01.T01 MTR_8g068870 34.921 126 79 1 30 155 162 284 9.71e-19 81.6
MsG0780036959.01.T01 MTR_0079s0070 42.857 98 55 1 37 133 139 236 5.16e-18 79.7
MsG0780036959.01.T01 MTR_4g038440 42.857 98 55 1 37 133 173 270 6.34e-18 79.7
MsG0780036959.01.T01 MTR_7g118320 31.410 156 94 3 9 155 132 283 2.12e-17 78.2
MsG0780036959.01.T01 MTR_7g118300 32.824 131 85 1 25 155 169 296 4.16e-17 77.4
MsG0780036959.01.T01 MTR_8g068690 34.862 109 71 0 25 133 170 278 4.92e-17 76.3
MsG0780036959.01.T01 MTR_8g068750 31.298 131 87 1 25 155 171 298 7.52e-17 76.6
MsG0780036959.01.T01 MTR_8g068570 38.144 97 60 0 37 133 156 252 7.86e-17 76.3
MsG0780036959.01.T01 MTR_3g021430 32.857 140 84 3 6 138 129 265 9.15e-17 76.3
MsG0780036959.01.T01 MTR_8g068690 32.258 124 84 0 25 148 170 293 1.06e-16 76.3
MsG0780036959.01.T01 MTR_8g024160 38.144 97 60 0 37 133 134 230 1.20e-16 75.9
MsG0780036959.01.T01 MTR_6g078850 30.534 131 88 1 25 155 177 304 3.30e-16 75.1
MsG0780036959.01.T01 MTR_3g021440 28.481 158 106 2 2 155 138 292 5.80e-16 73.9
MsG0780036959.01.T01 MTR_0017s0100 39.796 98 58 1 37 133 173 270 9.00e-16 73.6
MsG0780036959.01.T01 MTR_7g012020 36.364 77 49 0 29 105 167 243 3.57e-13 65.5
MsG0780036959.01.T01 MTR_3g096050 41.096 73 42 1 66 137 191 263 1.36e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036959.01.T01 AT5G54160 40.000 120 72 0 29 148 159 278 2.22e-23 94.7
MsG0780036959.01.T01 AT4G35150 38.843 121 71 2 30 148 114 233 1.02e-21 89.7
MsG0780036959.01.T01 AT1G51990 31.933 119 76 1 15 133 150 263 1.44e-17 78.6
MsG0780036959.01.T01 AT1G51990 31.933 119 76 1 15 133 150 263 1.64e-17 78.6
MsG0780036959.01.T01 AT1G77520 32.773 119 80 0 30 148 179 297 2.01e-16 75.5
MsG0780036959.01.T01 AT4G35160 38.017 121 72 2 30 148 171 290 1.10e-13 67.8
MsG0780036959.01.T01 AT3G53140 40.217 92 54 1 43 133 169 260 1.60e-12 64.3
MsG0780036959.01.T01 AT1G63140 33.898 118 73 1 17 134 191 303 1.66e-11 61.2
MsG0780036959.01.T01 AT5G37170 35.644 101 58 3 37 133 38 135 4.25e-11 59.7
MsG0780036959.01.T01 AT5G37170 35.644 101 58 3 37 133 138 235 4.72e-11 60.1
MsG0780036959.01.T01 AT1G33030 31.481 108 73 1 28 134 146 253 5.12e-11 59.7

Find 33 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACATATAGATTCGCAATT+AGG 0.203564 7:+17164992 None:intergenic
CTTCTTATCTATGTTACTTC+AGG 0.288092 7:+17164962 None:intergenic
CAATCTTTGAGACTACATTA+TGG 0.292380 7:-17165368 MsG0780036959.01.T01:CDS
GTTAATCCTTCTACCACATT+TGG 0.348422 7:+17165138 None:intergenic
AATCTTTGAGACTACATTAT+GGG 0.349412 7:-17165367 MsG0780036959.01.T01:CDS
AATATTATCAATTGAGCAAA+TGG 0.394667 7:-17165410 MsG0780036959.01.T01:CDS
TTAACTTCTTTGGTTGATGT+TGG 0.411610 7:-17165234 MsG0780036959.01.T01:CDS
ACTTCTTTGGTTGATGTTGG+AGG 0.420904 7:-17165231 MsG0780036959.01.T01:CDS
GACCTTCCAAATGTGGTAGA+AGG 0.429597 7:-17165144 MsG0780036959.01.T01:CDS
AAATGTGGTAGAAGGATTAA+CGG 0.461848 7:-17165136 MsG0780036959.01.T01:CDS
AATGTGGTAGAAGGATTAAC+GGG 0.469980 7:-17165135 MsG0780036959.01.T01:CDS
GTTGATGTTGGAGGTGGAAC+CGG 0.470193 7:-17165222 MsG0780036959.01.T01:CDS
TAATATTTATCAACCATGGT+AGG 0.470597 7:+17165426 None:intergenic
GTAAAATTGCATCAGCTTGA+GGG 0.481886 7:+17165067 None:intergenic
TTAATTCAAAGGGAAATGAA+TGG 0.484615 7:-17165032 MsG0780036959.01.T01:CDS
CAAGGTACGTATTAATTCAA+AGG 0.488981 7:-17165043 MsG0780036959.01.T01:CDS
AGTAAAATTGCATCAGCTTG+AGG 0.494453 7:+17165066 None:intergenic
CACGGTTAAAATTATTTGTG+AGG 0.517597 7:-17165196 MsG0780036959.01.T01:CDS
ATAGATTCGCAATTAGGTAA+AGG 0.519950 7:+17164998 None:intergenic
AATATTTATCAACCATGGTA+GGG 0.522593 7:+17165427 None:intergenic
AAGGTACGTATTAATTCAAA+GGG 0.523022 7:-17165042 MsG0780036959.01.T01:CDS
TCTTTGGTTGATGTTGGAGG+TGG 0.553343 7:-17165228 MsG0780036959.01.T01:CDS
ATCCTTCTACCACATTTGGA+AGG 0.561989 7:+17165142 None:intergenic
AATTGCATCAGCTTGAGGGA+TGG 0.574477 7:+17165071 None:intergenic
GAAGCTATTCAATGATGCTA+TGG 0.582775 7:-17165316 MsG0780036959.01.T01:CDS
TTGATAATATTTATCAACCA+TGG 0.592376 7:+17165422 None:intergenic
CTAATTGCGAATCTATATGT+CGG 0.595839 7:-17164991 MsG0780036959.01.T01:CDS
AGTGTTCGACCTTCCAAATG+TGG 0.610487 7:-17165151 MsG0780036959.01.T01:CDS
ATTGAGCAAATGGACCTCTA+CGG 0.614723 7:-17165400 MsG0780036959.01.T01:CDS
TGGAGGTGGAACCGGAAACA+CGG 0.653524 7:-17165214 MsG0780036959.01.T01:CDS
GATGGTAACAAACCCTACCA+TGG 0.661901 7:-17165439 MsG0780036959.01.T01:CDS
AAAGTGATTCTAATATGATG+AGG 0.664254 7:-17165293 MsG0780036959.01.T01:CDS
GATTGTGAGATCGTCCGTAG+AGG 0.681672 7:+17165386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTATCAATTGAGCAAA+TGG - Chr7:17165003-17165022 MsG0780036959.01.T01:CDS 20.0%
!!! AATAACTATCTAAGTTTTGT+CGG - Chr7:17165302-17165321 MsG0780036959.01.T01:CDS 20.0%
!!! TTGATAATATTTATCAACCA+TGG + Chr7:17164994-17165013 None:intergenic 20.0%
! AAAGTGATTCTAATATGATG+AGG - Chr7:17165120-17165139 MsG0780036959.01.T01:CDS 25.0%
! AAGGTACGTATTAATTCAAA+GGG - Chr7:17165371-17165390 MsG0780036959.01.T01:CDS 25.0%
! AATATTTATCAACCATGGTA+GGG + Chr7:17164989-17165008 None:intergenic 25.0%
! TAATATTTATCAACCATGGT+AGG + Chr7:17164990-17165009 None:intergenic 25.0%
! TTAATTCAAAGGGAAATGAA+TGG - Chr7:17165381-17165400 MsG0780036959.01.T01:CDS 25.0%
!!! AACACTATACATTTTAACGT+AGG + Chr7:17165248-17165267 None:intergenic 25.0%
!!! AATAATTTTAACCGTGTTTC+CGG + Chr7:17165213-17165232 None:intergenic 25.0%
!!! AATCTTTGAGACTACATTAT+GGG - Chr7:17165046-17165065 MsG0780036959.01.T01:CDS 25.0%
AAATGTGGTAGAAGGATTAA+CGG - Chr7:17165277-17165296 MsG0780036959.01.T01:CDS 30.0%
ATAGATTCGCAATTAGGTAA+AGG + Chr7:17165418-17165437 None:intergenic 30.0%
CAAGGTACGTATTAATTCAA+AGG - Chr7:17165370-17165389 MsG0780036959.01.T01:CDS 30.0%
CACGGTTAAAATTATTTGTG+AGG - Chr7:17165217-17165236 MsG0780036959.01.T01:CDS 30.0%
CTAATTGCGAATCTATATGT+CGG - Chr7:17165422-17165441 MsG0780036959.01.T01:CDS 30.0%
! GATTGCAAATCAGTTTTTGA+GGG - Chr7:17165155-17165174 MsG0780036959.01.T01:CDS 30.0%
! TGATTGCAAATCAGTTTTTG+AGG - Chr7:17165154-17165173 MsG0780036959.01.T01:CDS 30.0%
! TTAACTTCTTTGGTTGATGT+TGG - Chr7:17165179-17165198 MsG0780036959.01.T01:CDS 30.0%
!! CAATCTTTGAGACTACATTA+TGG - Chr7:17165045-17165064 MsG0780036959.01.T01:CDS 30.0%
AATGTGGTAGAAGGATTAAC+GGG - Chr7:17165278-17165297 MsG0780036959.01.T01:CDS 35.0%
AGTAAAATTGCATCAGCTTG+AGG + Chr7:17165350-17165369 None:intergenic 35.0%
CGACATATAGATTCGCAATT+AGG + Chr7:17165424-17165443 None:intergenic 35.0%
GAAGCTATTCAATGATGCTA+TGG - Chr7:17165097-17165116 MsG0780036959.01.T01:CDS 35.0%
GTAAAATTGCATCAGCTTGA+GGG + Chr7:17165349-17165368 None:intergenic 35.0%
GTTAATCCTTCTACCACATT+TGG + Chr7:17165278-17165297 None:intergenic 35.0%
! AGCTGATGCAATTTTACTCA+AGG - Chr7:17165352-17165371 MsG0780036959.01.T01:CDS 35.0%
!! AACTATCTAAGTTTTGTCGG+CGG - Chr7:17165305-17165324 MsG0780036959.01.T01:CDS 35.0%
!! TTTTGAGGGCTTAACTTCTT+TGG - Chr7:17165169-17165188 MsG0780036959.01.T01:CDS 35.0%
ATCCTTCTACCACATTTGGA+AGG + Chr7:17165274-17165293 None:intergenic 40.0%
ATTGAGCAAATGGACCTCTA+CGG - Chr7:17165013-17165032 MsG0780036959.01.T01:CDS 40.0%
! ACTTCTTTGGTTGATGTTGG+AGG - Chr7:17165182-17165201 MsG0780036959.01.T01:CDS 40.0%
AATTGCATCAGCTTGAGGGA+TGG + Chr7:17165345-17165364 None:intergenic 45.0%
AGTGTTCGACCTTCCAAATG+TGG - Chr7:17165262-17165281 MsG0780036959.01.T01:CDS 45.0%
GACCTTCCAAATGTGGTAGA+AGG - Chr7:17165269-17165288 MsG0780036959.01.T01:CDS 45.0%
! TCTTTGGTTGATGTTGGAGG+TGG - Chr7:17165185-17165204 MsG0780036959.01.T01:CDS 45.0%
GATTGTGAGATCGTCCGTAG+AGG + Chr7:17165030-17165049 None:intergenic 50.0%
GTTGATGTTGGAGGTGGAAC+CGG - Chr7:17165191-17165210 MsG0780036959.01.T01:CDS 50.0%
TGGAGGTGGAACCGGAAACA+CGG - Chr7:17165199-17165218 MsG0780036959.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17164975 17165460 17164975 ID=MsG0780036959.01;Name=MsG0780036959.01
Chr7 mRNA 17164975 17165460 17164975 ID=MsG0780036959.01.T01;Parent=MsG0780036959.01;Name=MsG0780036959.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|161
Chr7 exon 17164975 17165460 17164975 ID=MsG0780036959.01.T01:exon:3182;Parent=MsG0780036959.01.T01
Chr7 CDS 17164975 17165460 17164975 ID=MsG0780036959.01.T01:cds;Parent=MsG0780036959.01.T01
Gene Sequence

>MsG0780036959.01.T01

ATGGTAACAAACCCTACCATGGTTGATAAATATTATCAATTGAGCAAATGGACCTCTACGGACGATCTCACAATCTTTGAGACTACATTATGGGATTTCATGCAACAAGATTCGACTTATGCGAAGCTATTCAATGATGCTATGGAAAGTGATTCTAATATGATGAGGCTTGCAATGAGTGATTGCAAATCAGTTTTTGAGGGCTTAACTTCTTTGGTTGATGTTGGAGGTGGAACCGGAAACACGGTTAAAATTATTTGTGAGGCGTTTCCTACGTTAAAATGTATAGTGTTCGACCTTCCAAATGTGGTAGAAGGATTAACGGGAAATAACTATCTAAGTTTTGTCGGCGGAGATATGTTTGAATCCATCCCTCAAGCTGATGCAATTTTACTCAAGGTACGTATTAATTCAAAGGGAAATGAATGGTTTATTAATTTCCTTTACCTAATTGCGAATCTATATGTCGGAAAAAACCTGAAGTAA

Protein sequence

>MsG0780036959.01.T01

MVTNPTMVDKYYQLSKWTSTDDLTIFETTLWDFMQQDSTYAKLFNDAMESDSNMMRLAMSDCKSVFEGLTSLVDVGGGTGNTVKIICEAFPTLKCIVFDLPNVVEGLTGNNYLSFVGGDMFESIPQADAILLKVRINSKGNEWFINFLYLIANLYVGKNLK*