AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041194.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041194.01.T01 MTR_7g106330 95.833 120 5 0 71 190 4 123 2.29e-82 251
MsG0780041194.01.T01 MTR_7g106330 69.167 120 5 1 71 190 4 91 2.93e-50 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041194.01.T01 AT3G05675 65.455 110 37 1 82 190 15 124 1.00e-45 156
MsG0780041194.01.T01 AT3G05675 65.455 110 37 1 82 190 15 124 1.00e-45 156
MsG0780041194.01.T01 AT3G05675 65.455 110 37 1 82 190 15 124 1.00e-45 156

Find 32 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGGATCTTATGGCTTATT+TGG 0.124996 7:+87914669 None:intergenic
CAAGAATCAAAGGGATCTTA+TGG 0.269639 7:+87914660 None:intergenic
CACACACGCAGCAACAATTT+CGG 0.315925 7:+87913161 None:intergenic
GAGAAGGATATCTGTGTAAT+TGG 0.326783 7:-87913497 MsG0780041194.01.T01:CDS
AATGAGTATAGGAGAATATA+TGG 0.375280 7:-87914731 MsG0780041194.01.T01:CDS
AAGATTGTTATTACAGAATC+GGG 0.389033 7:+87914569 None:intergenic
AAAGATTGTTATTACAGAAT+CGG 0.391186 7:+87914568 None:intergenic
TAAAATCATGTATGGCAAGT+TGG 0.405983 7:-87914780 None:intergenic
AAAATCATGTATGGCAAGTT+GGG 0.420200 7:-87914779 None:intergenic
CTGATCGGCTATCTGAGAAT+TGG 0.421865 7:-87913367 MsG0780041194.01.T01:CDS
CGCAAGAAGGTCGAGATGAT+TGG 0.428744 7:-87913436 MsG0780041194.01.T01:CDS
TGGCCAACTTGTCAGATACT+AGG 0.460340 7:-87913347 MsG0780041194.01.T01:CDS
CCTCATTCTTCTCTCTCTCA+AGG 0.466215 7:-87914633 MsG0780041194.01.T01:CDS
CTTCTGTACGTTGTGGTCGA+TGG 0.471656 7:-87913257 MsG0780041194.01.T01:CDS
ATTATATCTAAAATCATGTA+TGG 0.474260 7:-87914788 None:intergenic
ATGAGGATTTCAAGAATCAA+AGG 0.495741 7:+87914650 None:intergenic
ATGGTTCAGTTGATGATTTG+TGG 0.499974 7:-87913238 MsG0780041194.01.T01:CDS
TCAGTTGATGATTTGTGGCA+TGG 0.516798 7:-87913233 MsG0780041194.01.T01:CDS
TACAGAATCGGGATTGAGCA+TGG 0.526319 7:+87914580 None:intergenic
GACACATAGCAAATGAGTAT+AGG 0.539897 7:-87914742 MsG0780041194.01.T01:CDS
TGAGGATTTCAAGAATCAAA+GGG 0.549223 7:+87914651 None:intergenic
GAACCTAGTATCTGACAAGT+TGG 0.564864 7:+87913344 None:intergenic
CCACAACATCACTAGTTGAT+CGG 0.567045 7:+87913471 None:intergenic
AATTAAGGATAAGGACGAGA+AGG 0.585063 7:-87913513 MsG0780041194.01.T01:intron
CATGGTGTTAGAAATGCTCT+TGG 0.588296 7:-87913215 MsG0780041194.01.T01:CDS
CCTTGAGAGAGAGAAGAATG+AGG 0.593674 7:+87914633 None:intergenic
GTGAGGCTACGCACGCAAGA+AGG 0.597661 7:-87913449 MsG0780041194.01.T01:CDS
TATTCGACTTCTGTACGTTG+TGG 0.606306 7:-87913264 MsG0780041194.01.T01:CDS
GAAGTCGAATAAAGTTTACA+TGG 0.621468 7:+87913276 None:intergenic
CCGATCAACTAGTGATGTTG+TGG 0.627570 7:-87913471 MsG0780041194.01.T01:CDS
TCGGGATTGAGCATGGAACA+AGG 0.641260 7:+87914587 None:intergenic
CAACTAGTGATGTTGTGGTG+AGG 0.729111 7:-87913466 MsG0780041194.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATGAAATTATAAAAACA+AGG + Chr7:87913505-87913524 None:intergenic 10.0%
!!! GTTTTTATAATTTCATTTTT+AGG - Chr7:87913506-87913525 MsG0780041194.01.T01:CDS 10.0%
!! TTCACTTCAATAATAATAAA+TGG + Chr7:87913946-87913965 None:intergenic 15.0%
!!! TCAATAATAATAAATGGATT+TGG + Chr7:87913940-87913959 None:intergenic 15.0%
!! AAGATTATCCTCTTTAATTA+AGG - Chr7:87914405-87914424 MsG0780041194.01.T01:intron 20.0%
!! AATATGTTTACTCTCAATTT+AGG + Chr7:87914036-87914055 None:intergenic 20.0%
!! AATTCTTTCATATCTTCAAT+TGG - Chr7:87913906-87913925 MsG0780041194.01.T01:intron 20.0%
!! ATATGAAATTTGAAGAAAAG+TGG + Chr7:87913594-87913613 None:intergenic 20.0%
!! TATTACTAATTACAGATAAG+AGG + Chr7:87914386-87914405 None:intergenic 20.0%
!!! AAAGATTGTTATTACAGAAT+CGG + Chr7:87913368-87913387 None:intergenic 20.0%
!!! TATCATTACTCCTAATTTTA+TGG - Chr7:87914212-87914231 MsG0780041194.01.T01:intron 20.0%
! AATGAGTATAGGAGAATATA+TGG - Chr7:87913202-87913221 MsG0780041194.01.T01:CDS 25.0%
! AGATATGAAAGAATTGACAT+AGG + Chr7:87913901-87913920 None:intergenic 25.0%
! AGTTAGAAAGATAAGGAAAT+AGG + Chr7:87913477-87913496 None:intergenic 25.0%
! ATAAACACTTGTGAATTGTT+TGG + Chr7:87913839-87913858 None:intergenic 25.0%
! ATCCTCTTTAATTAAGGATA+AGG - Chr7:87914411-87914430 MsG0780041194.01.T01:intron 25.0%
! ATGATATTTGTACAACCATT+TGG + Chr7:87914198-87914217 None:intergenic 25.0%
! CAAATAAACCAATCCAAAAA+TGG - Chr7:87913874-87913893 MsG0780041194.01.T01:intron 25.0%
! GATATGAAAGAATTGACATA+GGG + Chr7:87913900-87913919 None:intergenic 25.0%
! TAAACACTTGTGAATTGTTT+GGG + Chr7:87913838-87913857 None:intergenic 25.0%
! TCATAAAAGTTGAGACAAAA+TGG + Chr7:87914065-87914084 None:intergenic 25.0%
!! AAGATTGTTATTACAGAATC+GGG + Chr7:87913367-87913386 None:intergenic 25.0%
!! ATTCATATTATAGCTCTGTT+TGG - Chr7:87913614-87913633 MsG0780041194.01.T01:intron 25.0%
!! TTAAAGAGTGTAAAAAACCT+CGG + Chr7:87914342-87914361 None:intergenic 25.0%
!!! ATTGATTTTGTCCAAAACAA+GGG + Chr7:87914099-87914118 None:intergenic 25.0%
!!! TATTGATTTTGTCCAAAACA+AGG + Chr7:87914100-87914119 None:intergenic 25.0%
AAAGTGGAAAAGCAAACTTA+CGG + Chr7:87913578-87913597 None:intergenic 30.0%
AATACCGACACCATAAAATT+AGG + Chr7:87914225-87914244 None:intergenic 30.0%
ATGAGGATTTCAAGAATCAA+AGG + Chr7:87913286-87913305 None:intergenic 30.0%
CGTATAAGCTATAAGCTATT+TGG - Chr7:87913741-87913760 MsG0780041194.01.T01:intron 30.0%
GAAGTCGAATAAAGTTTACA+TGG + Chr7:87914660-87914679 None:intergenic 30.0%
GGATTAGAGTTAGAAAGATA+AGG + Chr7:87913484-87913503 None:intergenic 30.0%
GTCCTTATCCTTAATTAAAG+AGG + Chr7:87914416-87914435 None:intergenic 30.0%
TAACTTCTTCAAGATCCATA+CGG - Chr7:87913416-87913435 MsG0780041194.01.T01:CDS 30.0%
TGAGGATTTCAAGAATCAAA+GGG + Chr7:87913285-87913304 None:intergenic 30.0%
TTAGGTCGAAAAATCAAATG+CGG + Chr7:87913399-87913418 None:intergenic 30.0%
! AACTTGCTTCTTGATTTCAA+TGG - Chr7:87913546-87913565 MsG0780041194.01.T01:intron 30.0%
! AATAATAAATGGATTTGGCC+AGG + Chr7:87913935-87913954 None:intergenic 30.0%
! ATTGCAAGTATTTTGCTGAT+CGG - Chr7:87914551-87914570 MsG0780041194.01.T01:intron 30.0%
! TACTCCTAATTTTATGGTGT+CGG - Chr7:87914218-87914237 MsG0780041194.01.T01:intron 30.0%
! TTGATTTTGTCCAAAACAAG+GGG + Chr7:87914098-87914117 None:intergenic 30.0%
AAAATCAATAGAGCGAGAGA+GGG - Chr7:87914110-87914129 MsG0780041194.01.T01:intron 35.0%
AAGGGATCTTATGGCTTATT+TGG + Chr7:87913267-87913286 None:intergenic 35.0%
AATTAAGGATAAGGACGAGA+AGG - Chr7:87914420-87914439 MsG0780041194.01.T01:intron 35.0%
CAAGAATCAAAGGGATCTTA+TGG + Chr7:87913276-87913295 None:intergenic 35.0%
CATATCTTCAATTGGATTCC+TGG - Chr7:87913914-87913933 MsG0780041194.01.T01:intron 35.0%
GACACATAGCAAATGAGTAT+AGG - Chr7:87913191-87913210 MsG0780041194.01.T01:CDS 35.0%
GAGAAGGATATCTGTGTAAT+TGG - Chr7:87914436-87914455 MsG0780041194.01.T01:intron 35.0%
GTATGGATCTTGAAGAAGTT+AGG + Chr7:87913417-87913436 None:intergenic 35.0%
TCTCAAAAGTTGTCACCAAA+TGG - Chr7:87914180-87914199 MsG0780041194.01.T01:intron 35.0%
!! AATTGACATAGGGCCATTTT+TGG + Chr7:87913890-87913909 None:intergenic 35.0%
!! ATGGTTCAGTTGATGATTTG+TGG - Chr7:87914695-87914714 MsG0780041194.01.T01:CDS 35.0%
AGAGGAGAGACTCTGATTAT+TGG + Chr7:87914368-87914387 None:intergenic 40.0%
CAAAATCAATAGAGCGAGAG+AGG - Chr7:87914109-87914128 MsG0780041194.01.T01:intron 40.0%
CCAACTTCTTCTACTAACTG+AGG + Chr7:87914270-87914289 None:intergenic 40.0%
CCACAACATCACTAGTTGAT+CGG + Chr7:87914465-87914484 None:intergenic 40.0%
CCTCAGTTAGTAGAAGAAGT+TGG - Chr7:87914267-87914286 MsG0780041194.01.T01:intron 40.0%
CTAACTTCTTCTACTAACCG+AGG - Chr7:87914322-87914341 MsG0780041194.01.T01:intron 40.0%
GAACCTAGTATCTGACAAGT+TGG + Chr7:87914592-87914611 None:intergenic 40.0%
GAGGAGAGACTCTGATTATT+GGG + Chr7:87914367-87914386 None:intergenic 40.0%
GGTGAAAACAAGAACATGAG+TGG + Chr7:87913456-87913475 None:intergenic 40.0%
GTGAGAAATGAAAGACCGTA+TGG + Chr7:87913434-87913453 None:intergenic 40.0%
TATTCGACTTCTGTACGTTG+TGG - Chr7:87914669-87914688 MsG0780041194.01.T01:CDS 40.0%
TGGAAAAGCAAACTTACGGT+AGG + Chr7:87913574-87913593 None:intergenic 40.0%
! CATGGTGTTAGAAATGCTCT+TGG - Chr7:87914718-87914737 MsG0780041194.01.T01:CDS 40.0%
!! TCAGTTGATGATTTGTGGCA+TGG - Chr7:87914700-87914719 MsG0780041194.01.T01:CDS 40.0%
!!! ACATAGGGCCATTTTTGGAT+TGG + Chr7:87913885-87913904 None:intergenic 40.0%
!!! ATGGCTTATTTGGCTTTTGC+TGG + Chr7:87913257-87913276 None:intergenic 40.0%
CAACTAGTGATGTTGTGGTG+AGG - Chr7:87914467-87914486 MsG0780041194.01.T01:intron 45.0%
CACACACGCAGCAACAATTT+CGG + Chr7:87914775-87914794 None:intergenic 45.0%
CCGATCAACTAGTGATGTTG+TGG - Chr7:87914462-87914481 MsG0780041194.01.T01:intron 45.0%
CCTCATTCTTCTCTCTCTCA+AGG - Chr7:87913300-87913319 MsG0780041194.01.T01:CDS 45.0%
CCTTGAGAGAGAGAAGAATG+AGG + Chr7:87913303-87913322 None:intergenic 45.0%
CTGATCGGCTATCTGAGAAT+TGG - Chr7:87914566-87914585 MsG0780041194.01.T01:intron 45.0%
TACAGAATCGGGATTGAGCA+TGG + Chr7:87913356-87913375 None:intergenic 45.0%
TGGCCAACTTGTCAGATACT+AGG - Chr7:87914586-87914605 MsG0780041194.01.T01:CDS 45.0%
TGTCCAAAACAAGGGGATGA+AGG + Chr7:87914091-87914110 None:intergenic 45.0%
!! CAACCTTCATCCCCTTGTTT+TGG - Chr7:87914085-87914104 MsG0780041194.01.T01:intron 45.0%
AATAGAGCGAGAGAGGGAGA+GGG - Chr7:87914116-87914135 MsG0780041194.01.T01:intron 50.0%
CGCAAGAAGGTCGAGATGAT+TGG - Chr7:87914497-87914516 MsG0780041194.01.T01:intron 50.0%
CTTCTGTACGTTGTGGTCGA+TGG - Chr7:87914676-87914695 MsG0780041194.01.T01:CDS 50.0%
TCGGGATTGAGCATGGAACA+AGG + Chr7:87913349-87913368 None:intergenic 50.0%
CAATAGAGCGAGAGAGGGAG+AGG - Chr7:87914115-87914134 MsG0780041194.01.T01:intron 55.0%
GTGAGGCTACGCACGCAAGA+AGG - Chr7:87914484-87914503 MsG0780041194.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87913160 87914795 87913160 ID=MsG0780041194.01;Name=MsG0780041194.01
Chr7 mRNA 87913160 87914795 87913160 ID=MsG0780041194.01.T01;Parent=MsG0780041194.01;Name=MsG0780041194.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0.5|0.66|1|0.5|0.33|3|0|193
Chr7 exon 87913160 87913528 87913160 ID=MsG0780041194.01.T01:exon:3298;Parent=MsG0780041194.01.T01
Chr7 exon 87914583 87914795 87914583 ID=MsG0780041194.01.T01:exon:3297;Parent=MsG0780041194.01.T01
Chr7 CDS 87914583 87914795 87914583 ID=MsG0780041194.01.T01:cds;Parent=MsG0780041194.01.T01
Chr7 CDS 87913160 87913528 87913160 ID=MsG0780041194.01.T01:cds;Parent=MsG0780041194.01.T01
Gene Sequence

>MsG0780041194.01.T01

ATGTATGGCAAGTTGGGAAGTAACAAGAATAGACACATAGCAAATGAGTATAGGAGAATATATGGTACTACTACTATGAGTAGACAAACGCACGCCAGCAAAAGCCAAATAAGCCATAAGATCCCTTTGATTCTTGAAATCCTCATTCTTCTCTCTCTCAAGGTTTTTGCTTCTTCGTTTCATTCACCTTGTTCCATGCTCAATCCCGATTCTGATAAGGACGAGAAGGATATCTGTGTAATTGGTGACCGATCAACTAGTGATGTTGTGGTGAGGCTACGCACGCAAGAAGGTCGAGATGATTGGTTGTACTGTCACTCAGCGATACTTGTCGAAAATTGCAAGTATTTTGCTGATCGGCTATCTGAGAATTGGCCAACTTGTCAGATACTAGGTTCGCGCAACTGTGTTGATGTTAATTGTCAAGAATCAGATTTCGATTACCATGTAAACTTTATTCGACTTCTGTACGTTGTGGTCGATGGTTCAGTTGATGATTTGTGGCATGGTGTTAGAAATGCTCTTGGCATTCTTAAAGTAGCTGTTGAGCTTGAATGTCCGAAATTGTTGCTGCGTGTGTGA

Protein sequence

>MsG0780041194.01.T01

MYGKLGSNKNRHIANEYRRIYGTTTMSRQTHASKSQISHKIPLILEILILLSLKVFASSFHSPCSMLNPDSDKDEKDICVIGDRSTSDVVVRLRTQEGRDDWLYCHSAILVENCKYFADRLSENWPTCQILGSRNCVDVNCQESDFDYHVNFIRLLYVVVDGSVDDLWHGVRNALGILKVAVELECPKLLLRV*