Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041193.01.T01 | KEH24247.1 | 98.81 | 84 | 1 | 0 | 1 | 84 | 101 | 184 | 2.56E-48 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041193.01.T01 | Q8RX01 | 75.61 | 82 | 20 | 0 | 3 | 84 | 136 | 217 | 7.72E-32 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041193.01.T01 | A0A072U3E1 | 98.810 | 84 | 1 | 0 | 1 | 84 | 101 | 184 | 1.22e-48 | 167 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048383.01 | MsG0780041193.01 | 0.810582 | 1.073067e-50 | 6.493159e-48 |
| MsG0080048968.01 | MsG0780041193.01 | 0.804778 | 1.834574e-49 | 9.542907e-47 |
| MsG0180000144.01 | MsG0780041193.01 | 0.802894 | 4.518119e-49 | 2.239056e-46 |
| MsG0180000611.01 | MsG0780041193.01 | 0.816398 | 5.644451e-52 | 3.995224e-49 |
| MsG0180000680.01 | MsG0780041193.01 | 0.817088 | 3.952166e-52 | 2.850725e-49 |
| MsG0180000741.01 | MsG0780041193.01 | 0.808112 | 3.634525e-50 | 2.060371e-47 |
| MsG0180000836.01 | MsG0780041193.01 | 0.818756 | 1.660148e-52 | 1.253662e-49 |
| MsG0180001173.01 | MsG0780041193.01 | 0.825011 | 5.915131e-54 | 5.326818e-51 |
| MsG0180001311.01 | MsG0780041193.01 | 0.814412 | 1.560776e-51 | 1.046610e-48 |
| MsG0180001566.01 | MsG0780041193.01 | 0.813188 | 2.904883e-51 | 1.884026e-48 |
| MsG0180001662.01 | MsG0780041193.01 | 0.816913 | 4.326718e-52 | 3.105986e-49 |
| MsG0180002992.01 | MsG0780041193.01 | 0.803368 | 3.604976e-49 | 1.808234e-46 |
| MsG0180003303.01 | MsG0780041193.01 | 0.800064 | 1.717466e-48 | 7.921414e-46 |
| MsG0180004016.01 | MsG0780041193.01 | 0.815277 | 1.003747e-51 | 6.889925e-49 |
| MsG0180004128.01 | MsG0780041193.01 | 0.811479 | 6.860115e-51 | 4.251131e-48 |
| MsG0180004160.01 | MsG0780041193.01 | 0.814466 | 1.518680e-51 | 1.019840e-48 |
| MsG0180004437.01 | MsG0780041193.01 | 0.812909 | 3.343253e-51 | 2.152231e-48 |
| MsG0180004767.01 | MsG0780041193.01 | 0.809408 | 1.920429e-50 | 1.126692e-47 |
| MsG0180005233.01 | MsG0780041193.01 | 0.800028 | 1.746049e-48 | 8.045908e-46 |
| MsG0180005304.01 | MsG0780041193.01 | 0.801292 | 9.646817e-49 | 4.590095e-46 |
| MsG0180005443.01 | MsG0780041193.01 | 0.809382 | 1.945661e-50 | 1.140687e-47 |
| MsG0180005451.01 | MsG0780041193.01 | 0.816342 | 5.811548e-52 | 4.107357e-49 |
| MsG0280006367.01 | MsG0780041193.01 | 0.804257 | 2.355620e-49 | 1.208894e-46 |
| MsG0280006368.01 | MsG0780041193.01 | 0.813175 | 2.923487e-51 | 1.895414e-48 |
| MsG0780040418.01 | MsG0780041193.01 | 0.801556 | 8.515251e-49 | 4.078682e-46 |
| MsG0780040856.01 | MsG0780041193.01 | 0.808326 | 3.272069e-50 | 1.865518e-47 |
| MsG0780041127.01 | MsG0780041193.01 | 0.808147 | 3.572732e-50 | 2.027283e-47 |
| MsG0780041192.01 | MsG0780041193.01 | 0.843891 | 1.060266e-58 | 1.682853e-55 |
| MsG0780041193.01 | MsG0780041194.01 | 0.860066 | 2.676458e-63 | 7.224284e-60 |
| MsG0780041193.01 | MsG0780041258.01 | 0.826512 | 2.605486e-54 | 2.448560e-51 |
| MsG0780041193.01 | MsG0780041300.01 | 0.871796 | 5.199555e-67 | 2.124650e-63 |
| MsG0780041193.01 | MsG0780041305.01 | 0.806162 | 9.403324e-50 | 5.068321e-47 |
| MsG0780041193.01 | MsG0780041390.01 | 0.852771 | 3.708222e-61 | 7.832885e-58 |
| MsG0780041193.01 | MsG0880042760.01 | 0.834591 | 2.744110e-56 | 3.271438e-53 |
| MsG0780041193.01 | MsG0880045460.01 | 0.810439 | 1.151979e-50 | 6.943959e-48 |
| MsG0780041193.01 | MsG0880046027.01 | 0.815184 | 1.052772e-51 | 7.207551e-49 |
| MsG0780041193.01 | MsG0880046074.01 | 0.806107 | 9.659332e-50 | 5.198802e-47 |
| MsG0780041193.01 | MsG0880046538.01 | 0.806051 | 9.922223e-50 | 5.332766e-47 |
| MsG0780041193.01 | MsG0880047428.01 | 0.814582 | 1.431719e-51 | 9.644242e-49 |
| MsG0380013579.01 | MsG0780041193.01 | 0.807320 | 5.354022e-50 | 2.973080e-47 |
| MsG0380014576.01 | MsG0780041193.01 | 0.820310 | 7.337645e-53 | 5.785311e-50 |
| MsG0680031654.01 | MsG0780041193.01 | 0.803977 | 2.694303e-49 | 1.372905e-46 |
| MsG0680031699.01 | MsG0780041193.01 | 0.818038 | 2.414292e-52 | 1.787311e-49 |
| MsG0680032075.01 | MsG0780041193.01 | 0.821239 | 4.487672e-53 | 3.630858e-50 |
| MsG0680033392.01 | MsG0780041193.01 | 0.821741 | 3.435489e-53 | 2.818804e-50 |
| MsG0680034476.01 | MsG0780041193.01 | 0.811657 | 6.272737e-51 | 3.905177e-48 |
| MsG0680035306.01 | MsG0780041193.01 | 0.833642 | 4.742678e-56 | 5.494993e-53 |
| MsG0480019148.01 | MsG0780041193.01 | 0.803701 | 3.074824e-49 | 1.555613e-46 |
| MsG0480020331.01 | MsG0780041193.01 | 0.804966 | 1.676177e-49 | 8.761333e-47 |
| MsG0480020540.01 | MsG0780041193.01 | 0.807344 | 5.292861e-50 | 2.940823e-47 |
| MsG0480021190.01 | MsG0780041193.01 | 0.813520 | 2.455147e-51 | 1.606936e-48 |
| MsG0480022203.01 | MsG0780041193.01 | 0.803977 | 2.694303e-49 | 1.372905e-46 |
| MsG0480023377.01 | MsG0780041193.01 | 0.807429 | 5.076090e-50 | 2.826692e-47 |
| MsG0480023659.01 | MsG0780041193.01 | 0.823091 | 1.668932e-53 | 1.423087e-50 |
| MsG0580025651.01 | MsG0780041193.01 | 0.821633 | 3.640239e-53 | 2.977668e-50 |
| MsG0380015220.01 | MsG0780041193.01 | 0.828343 | 9.477125e-55 | 9.393454e-52 |
| MsG0380015893.01 | MsG0780041193.01 | 0.806519 | 7.909695e-50 | 4.302065e-47 |
| MsG0380015980.01 | MsG0780041193.01 | 0.839964 | 1.156509e-57 | 1.623398e-54 |
| MsG0380016905.01 | MsG0780041193.01 | 0.826874 | 2.135422e-54 | 2.027749e-51 |
| MsG0380017476.01 | MsG0780041193.01 | 0.816326 | 5.857123e-52 | 4.137825e-49 |
| MsG0480018292.01 | MsG0780041193.01 | 0.850141 | 2.056595e-60 | 3.989379e-57 |
| MsG0580029283.01 | MsG0780041193.01 | 0.802464 | 5.540393e-49 | 2.715813e-46 |
| MsG0580029444.01 | MsG0780041193.01 | 0.803520 | 3.352735e-49 | 1.688422e-46 |
| MsG0580029801.01 | MsG0780041193.01 | 0.821756 | 3.408571e-53 | 2.797951e-50 |
| MsG0580029919.01 | MsG0780041193.01 | 0.808245 | 3.404127e-50 | 1.936681e-47 |
| MsG0580030204.01 | MsG0780041193.01 | 0.815907 | 7.267473e-52 | 5.075108e-49 |
| MsG0280006679.01 | MsG0780041193.01 | 0.815551 | 8.725921e-52 | 6.034717e-49 |
| MsG0280006821.01 | MsG0780041193.01 | 0.813239 | 2.830044e-51 | 1.838022e-48 |
| MsG0280006852.01 | MsG0780041193.01 | 0.805391 | 1.365221e-49 | 7.214010e-47 |
| MsG0280006865.01 | MsG0780041193.01 | 0.812633 | 3.843937e-51 | 2.456666e-48 |
| MsG0280007441.01 | MsG0780041193.01 | 0.830137 | 3.478681e-55 | 3.633354e-52 |
| MsG0280007710.01 | MsG0780041193.01 | 0.825542 | 4.428418e-54 | 4.048280e-51 |
| MsG0280008004.01 | MsG0780041193.01 | 0.843499 | 1.349594e-58 | 2.115590e-55 |
| MsG0280009575.01 | MsG0780041193.01 | 0.800875 | 1.173879e-48 | 5.527066e-46 |
| MsG0280010109.01 | MsG0780041193.01 | 0.813319 | 2.718744e-51 | 1.769694e-48 |
| MsG0280010411.01 | MsG0780041193.01 | 0.817379 | 3.399315e-52 | 2.471657e-49 |
| MsG0780036240.01 | MsG0780041193.01 | 0.804909 | 1.722535e-49 | 8.990979e-47 |
| MsG0780038443.01 | MsG0780041193.01 | 0.800602 | 1.334725e-48 | 6.240716e-46 |
| MsG0780039717.01 | MsG0780041193.01 | 0.803680 | 3.105889e-49 | 1.570473e-46 |
| MsG0780039828.01 | MsG0780041193.01 | 0.800775 | 1.230092e-48 | 5.776731e-46 |
| MsG0780039943.01 | MsG0780041193.01 | 0.807177 | 5.740087e-50 | 3.175548e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041193.01.T01 | MTR_7g106330 | 98.810 | 84 | 1 | 0 | 1 | 84 | 101 | 184 | 3.10e-52 | 167 |
| MsG0780041193.01.T01 | MTR_7g106330 | 98.810 | 84 | 1 | 0 | 1 | 84 | 133 | 216 | 4.52e-52 | 168 |
| MsG0780041193.01.T01 | MTR_3g011800 | 50.000 | 60 | 27 | 1 | 28 | 84 | 6 | 65 | 1.59e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041193.01.T01 | AT3G05675 | 75.610 | 82 | 20 | 0 | 3 | 84 | 136 | 217 | 7.87e-33 | 117 |
| MsG0780041193.01.T01 | AT3G05675 | 75.610 | 82 | 20 | 0 | 3 | 84 | 136 | 217 | 7.87e-33 | 117 |
| MsG0780041193.01.T01 | AT3G05675 | 75.610 | 82 | 20 | 0 | 3 | 84 | 136 | 217 | 7.87e-33 | 117 |
Find 22 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAAAATTGTACCGAGAA+TGG | 0.375149 | 7:-87913091 | MsG0780041193.01.T01:CDS |
| ATATGTTTCTGATAGCTAAC+TGG | 0.387088 | 7:+87913025 | None:intergenic |
| CTGATAGCTAACTGGTTAAC+CGG | 0.398560 | 7:+87913033 | None:intergenic |
| TTGAAAATTGTACCGAGAAT+GGG | 0.421104 | 7:-87913090 | MsG0780041193.01.T01:CDS |
| GATTTCAGATCATTCATTGC+CGG | 0.460656 | 7:+87912964 | None:intergenic |
| TATCATCAGCGATCAACAAA+GGG | 0.477424 | 7:+87912893 | None:intergenic |
| TGCTACATCGTCGCCTCCAC+CGG | 0.488415 | 7:-87912983 | MsG0780041193.01.T01:CDS |
| AGATCATTCATTGCCGGTGG+AGG | 0.499953 | 7:+87912970 | None:intergenic |
| TATCCTAGCTAGACTTCAAC+CGG | 0.505035 | 7:-87913052 | MsG0780041193.01.T01:CDS |
| TCAGCTTGTAATCCCATTCT+CGG | 0.521850 | 7:+87913078 | None:intergenic |
| TATCAGAAACATATTTCTCT+CGG | 0.549893 | 7:-87913016 | MsG0780041193.01.T01:CDS |
| TAACCGGTTGAAGTCTAGCT+AGG | 0.567773 | 7:+87913049 | None:intergenic |
| TTCAGATCATTCATTGCCGG+TGG | 0.572901 | 7:+87912967 | None:intergenic |
| GGTTGAAGTCTAGCTAGGAT+AGG | 0.590203 | 7:+87913054 | None:intergenic |
| ATATCATCAGCGATCAACAA+AGG | 0.597255 | 7:+87912892 | None:intergenic |
| AATACTTGGAAGCAATGACA+TGG | 0.605753 | 7:-87913137 | None:intergenic |
| CGACGATGTAGCAAAACGGA+TGG | 0.632476 | 7:+87912993 | None:intergenic |
| GAGGCGACGATGTAGCAAAA+CGG | 0.635778 | 7:+87912989 | None:intergenic |
| ATACTTGGAAGCAATGACAT+GGG | 0.673251 | 7:-87913136 | None:intergenic |
| GACATGGGAAGAGAGTGAAG+AGG | 0.683633 | 7:-87913121 | MsG0780041193.01.T01:CDS |
| ATCATCAGCGATCAACAAAG+GGG | 0.702082 | 7:+87912894 | None:intergenic |
| ACTTGAGTACATGCTAACTG+AGG | 0.740737 | 7:-87912926 | MsG0780041193.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATCAGAAACATATTTCTCT+CGG | - | Chr7:87912995-87913014 | MsG0780041193.01.T01:CDS | 25.0% |
| TTGAAAATTGTACCGAGAAT+GGG | - | Chr7:87912921-87912940 | MsG0780041193.01.T01:CDS | 30.0% | |
| TTTGAAAATTGTACCGAGAA+TGG | - | Chr7:87912920-87912939 | MsG0780041193.01.T01:CDS | 30.0% | |
| ! | ATATGTTTCTGATAGCTAAC+TGG | + | Chr7:87912989-87913008 | None:intergenic | 30.0% |
| ATATCATCAGCGATCAACAA+AGG | + | Chr7:87913122-87913141 | None:intergenic | 35.0% | |
| GATTTCAGATCATTCATTGC+CGG | + | Chr7:87913050-87913069 | None:intergenic | 35.0% | |
| TATCATCAGCGATCAACAAA+GGG | + | Chr7:87913121-87913140 | None:intergenic | 35.0% | |
| ACTTGAGTACATGCTAACTG+AGG | - | Chr7:87913085-87913104 | MsG0780041193.01.T01:CDS | 40.0% | |
| ATCATCAGCGATCAACAAAG+GGG | + | Chr7:87913120-87913139 | None:intergenic | 40.0% | |
| CTGATAGCTAACTGGTTAAC+CGG | + | Chr7:87912981-87913000 | None:intergenic | 40.0% | |
| TATCCTAGCTAGACTTCAAC+CGG | - | Chr7:87912959-87912978 | MsG0780041193.01.T01:CDS | 40.0% | |
| TCAGCTTGTAATCCCATTCT+CGG | + | Chr7:87912936-87912955 | None:intergenic | 40.0% | |
| GGTTGAAGTCTAGCTAGGAT+AGG | + | Chr7:87912960-87912979 | None:intergenic | 45.0% | |
| TAACCGGTTGAAGTCTAGCT+AGG | + | Chr7:87912965-87912984 | None:intergenic | 45.0% | |
| TTCAGATCATTCATTGCCGG+TGG | + | Chr7:87913047-87913066 | None:intergenic | 45.0% | |
| AGATCATTCATTGCCGGTGG+AGG | + | Chr7:87913044-87913063 | None:intergenic | 50.0% | |
| CGACGATGTAGCAAAACGGA+TGG | + | Chr7:87913021-87913040 | None:intergenic | 50.0% | |
| GACATGGGAAGAGAGTGAAG+AGG | - | Chr7:87912890-87912909 | MsG0780041193.01.T01:CDS | 50.0% | |
| GAGGCGACGATGTAGCAAAA+CGG | + | Chr7:87913025-87913044 | None:intergenic | 50.0% | |
| TGCTACATCGTCGCCTCCAC+CGG | - | Chr7:87913028-87913047 | MsG0780041193.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 87912888 | 87913145 | 87912888 | ID=MsG0780041193.01;Name=MsG0780041193.01 |
| Chr7 | mRNA | 87912888 | 87913145 | 87912888 | ID=MsG0780041193.01.T01;Parent=MsG0780041193.01;Name=MsG0780041193.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|85 |
| Chr7 | exon | 87912888 | 87913145 | 87912888 | ID=MsG0780041193.01.T01:exon:3296;Parent=MsG0780041193.01.T01 |
| Chr7 | CDS | 87912888 | 87913145 | 87912888 | ID=MsG0780041193.01.T01:cds;Parent=MsG0780041193.01.T01 |
| Gene Sequence |
| Protein sequence |
