AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041192.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041192.01.T01 MTR_7g106330 97.561 164 4 0 1 164 244 407 2.79e-113 327
MsG0780041192.01.T01 MTR_7g106330 97.561 164 4 0 1 164 276 439 7.68e-113 327
MsG0780041192.01.T01 MTR_3g011800 45.161 155 78 3 1 153 74 223 4.03e-42 140
MsG0780041192.01.T01 MTR_3g011800 45.161 155 78 3 1 153 124 273 1.04e-41 141
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041192.01.T01 AT3G05675 63.473 167 55 4 1 163 277 441 6.45e-64 202
MsG0780041192.01.T01 AT3G05675 63.473 167 55 4 1 163 277 441 6.45e-64 202
MsG0780041192.01.T01 AT3G05675 63.473 167 55 4 1 163 277 441 6.45e-64 202
MsG0780041192.01.T01 AT4G10800 45.513 156 76 4 1 153 97 246 2.15e-39 134
MsG0780041192.01.T01 AT4G10800 45.513 156 76 4 1 153 120 269 3.14e-39 134

Find 36 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCTTGCATTGCCATC+AGG 0.346115 7:-87912376 MsG0780041192.01.T01:CDS
GGATGCTGCGTTGCTTAAAA+TGG 0.377981 7:-87912440 MsG0780041192.01.T01:CDS
TGACTGAGGCATTCGAAGTA+TGG 0.393117 7:-87912294 MsG0780041192.01.T01:CDS
TGCATTGCCATCAGGGGATC+AGG 0.394170 7:-87912368 MsG0780041192.01.T01:CDS
GGTTGGAAAACGAGAATATA+CGG 0.406032 7:-87912327 MsG0780041192.01.T01:CDS
GGCAGAATCAATCACTGGTT+TGG 0.436374 7:+87912477 None:intergenic
GAGAAGACTCTCATTGTTAG+AGG 0.440113 7:-87912248 MsG0780041192.01.T01:CDS
TGGCGAAATGTGGCAATCTC+TGG 0.442679 7:-87912416 MsG0780041192.01.T01:CDS
TTGCCATCAGGGGATCAGGC+AGG 0.460011 7:-87912364 MsG0780041192.01.T01:CDS
AACCTTTGATGTTACCTCAA+TGG 0.460071 7:+87912588 None:intergenic
TCGAATGCCTCAGTCAGGTC+TGG 0.468523 7:+87912301 None:intergenic
AAGAATGATAGAGACAAAAC+TGG 0.473112 7:+87912390 None:intergenic
ACCTCAAGACGTTTCGCAGT+TGG 0.501923 7:+87912532 None:intergenic
GGTTTGGTAACCCTCACAAA+TGG 0.504480 7:+87912493 None:intergenic
GAGTCTTCTCCTTGCAACCT+TGG 0.515051 7:+87912261 None:intergenic
ATACTTCGAATGCCTCAGTC+AGG 0.520495 7:+87912296 None:intergenic
TCATTCTTGCATTGCCATCA+GGG 0.532419 7:-87912375 MsG0780041192.01.T01:CDS
TGCCATCAGGGGATCAGGCA+GGG 0.532443 7:-87912363 MsG0780041192.01.T01:CDS
AATTGTGGCTTCCATTTGTG+AGG 0.547715 7:-87912504 MsG0780041192.01.T01:CDS
TGAGTACTGGTTTGATGCAT+CGG 0.555681 7:-87912683 MsG0780041192.01.T01:CDS
GATAAAGTTGCGAGCCATTG+AGG 0.559973 7:-87912602 MsG0780041192.01.T01:CDS
GTTATAGATCCAAGGTTGCA+AGG 0.561101 7:-87912270 MsG0780041192.01.T01:CDS
GTCATGGCAGAATCAATCAC+TGG 0.572683 7:+87912472 None:intergenic
AGCCATTGAGGTAACATCAA+AGG 0.577241 7:-87912590 MsG0780041192.01.T01:CDS
CATTCTTGCATTGCCATCAG+GGG 0.594583 7:-87912374 MsG0780041192.01.T01:CDS
AGCCCTGCCTGATCCCCTGA+TGG 0.596176 7:+87912361 None:intergenic
GCCAACTGCGAAACGTCTTG+AGG 0.600096 7:-87912533 MsG0780041192.01.T01:CDS
AGTATGGTGTTATAGATCCA+AGG 0.604280 7:-87912278 MsG0780041192.01.T01:CDS
GCTGCGTTGCTTAAAATGGA+TGG 0.619104 7:-87912436 MsG0780041192.01.T01:CDS
ACTGGTTTGATGCATCGGAA+AGG 0.623195 7:-87912678 MsG0780041192.01.T01:CDS
AGCATCCTCACAATTCGTCA+TGG 0.633088 7:+87912456 None:intergenic
ATTGTGGCTTCCATTTGTGA+GGG 0.644880 7:-87912503 MsG0780041192.01.T01:CDS
TTAAAATGGATGGCGAAATG+TGG 0.673164 7:-87912426 MsG0780041192.01.T01:CDS
AAAGTACTTGAACTTGAACA+AGG 0.686492 7:-87912649 MsG0780041192.01.T01:CDS
TTCTGCCATGACGAATTGTG+AGG 0.703220 7:-87912461 MsG0780041192.01.T01:CDS
ACGGTATCCAGACCTGACTG+AGG 0.735150 7:-87912308 MsG0780041192.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAATGATAGAGACAAAAC+TGG + Chr7:87912535-87912554 None:intergenic 30.0%
! AAAGTACTTGAACTTGAACA+AGG - Chr7:87912273-87912292 MsG0780041192.01.T01:CDS 30.0%
AACCTTTGATGTTACCTCAA+TGG + Chr7:87912337-87912356 None:intergenic 35.0%
GGTTGGAAAACGAGAATATA+CGG - Chr7:87912595-87912614 MsG0780041192.01.T01:CDS 35.0%
TTAAAATGGATGGCGAAATG+TGG - Chr7:87912496-87912515 MsG0780041192.01.T01:CDS 35.0%
! TGAGAGATTTTGTTGAGTAC+TGG - Chr7:87912226-87912245 MsG0780041192.01.T01:CDS 35.0%
!! AGTATGGTGTTATAGATCCA+AGG - Chr7:87912644-87912663 MsG0780041192.01.T01:CDS 35.0%
!! GGTAACATCAAAGGTTTTAG+AGG - Chr7:87912341-87912360 MsG0780041192.01.T01:CDS 35.0%
!!! GTCTTGAGGTTTTGAAATTG+TGG - Chr7:87912403-87912422 MsG0780041192.01.T01:CDS 35.0%
!!! GTTTTAGAGGCAATTGCTTA+TGG - Chr7:87912354-87912373 MsG0780041192.01.T01:CDS 35.0%
AATTGTGGCTTCCATTTGTG+AGG - Chr7:87912418-87912437 MsG0780041192.01.T01:CDS 40.0%
AGCCATTGAGGTAACATCAA+AGG - Chr7:87912332-87912351 MsG0780041192.01.T01:CDS 40.0%
ATCATTCTTGCATTGCCATC+AGG - Chr7:87912546-87912565 MsG0780041192.01.T01:CDS 40.0%
ATTGTGGCTTCCATTTGTGA+GGG - Chr7:87912419-87912438 MsG0780041192.01.T01:CDS 40.0%
GAGAAGACTCTCATTGTTAG+AGG - Chr7:87912674-87912693 MsG0780041192.01.T01:CDS 40.0%
GTTATAGATCCAAGGTTGCA+AGG - Chr7:87912652-87912671 MsG0780041192.01.T01:CDS 40.0%
TCATTCTTGCATTGCCATCA+GGG - Chr7:87912547-87912566 MsG0780041192.01.T01:CDS 40.0%
!! AGGGCTTTTAACTGAATGGT+TGG - Chr7:87912578-87912597 MsG0780041192.01.T01:CDS 40.0%
!! TGAGTACTGGTTTGATGCAT+CGG - Chr7:87912239-87912258 MsG0780041192.01.T01:CDS 40.0%
AGCATCCTCACAATTCGTCA+TGG + Chr7:87912469-87912488 None:intergenic 45.0%
ATACTTCGAATGCCTCAGTC+AGG + Chr7:87912629-87912648 None:intergenic 45.0%
CATTCTTGCATTGCCATCAG+GGG - Chr7:87912548-87912567 MsG0780041192.01.T01:CDS 45.0%
GATAAAGTTGCGAGCCATTG+AGG - Chr7:87912320-87912339 MsG0780041192.01.T01:CDS 45.0%
GCTGCGTTGCTTAAAATGGA+TGG - Chr7:87912486-87912505 MsG0780041192.01.T01:CDS 45.0%
GGATGCTGCGTTGCTTAAAA+TGG - Chr7:87912482-87912501 MsG0780041192.01.T01:CDS 45.0%
GTCATGGCAGAATCAATCAC+TGG + Chr7:87912453-87912472 None:intergenic 45.0%
TTCTGCCATGACGAATTGTG+AGG - Chr7:87912461-87912480 MsG0780041192.01.T01:CDS 45.0%
! GGCAGAATCAATCACTGGTT+TGG + Chr7:87912448-87912467 None:intergenic 45.0%
! GGTTTGGTAACCCTCACAAA+TGG + Chr7:87912432-87912451 None:intergenic 45.0%
! TGACTGAGGCATTCGAAGTA+TGG - Chr7:87912628-87912647 MsG0780041192.01.T01:CDS 45.0%
!! ACTGGTTTGATGCATCGGAA+AGG - Chr7:87912244-87912263 MsG0780041192.01.T01:CDS 45.0%
!! AGGCAGGGCTTTTAACTGAA+TGG - Chr7:87912574-87912593 MsG0780041192.01.T01:CDS 45.0%
ACCTCAAGACGTTTCGCAGT+TGG + Chr7:87912393-87912412 None:intergenic 50.0%
GAGTCTTCTCCTTGCAACCT+TGG + Chr7:87912664-87912683 None:intergenic 50.0%
TGGCGAAATGTGGCAATCTC+TGG - Chr7:87912506-87912525 MsG0780041192.01.T01:CDS 50.0%
ACGGTATCCAGACCTGACTG+AGG - Chr7:87912614-87912633 MsG0780041192.01.T01:CDS 55.0%
GCCAACTGCGAAACGTCTTG+AGG - Chr7:87912389-87912408 MsG0780041192.01.T01:CDS 55.0%
TCGAATGCCTCAGTCAGGTC+TGG + Chr7:87912624-87912643 None:intergenic 55.0%
TGCATTGCCATCAGGGGATC+AGG - Chr7:87912554-87912573 MsG0780041192.01.T01:CDS 55.0%
TGCCATCAGGGGATCAGGCA+GGG - Chr7:87912559-87912578 MsG0780041192.01.T01:CDS 60.0%
TTGCCATCAGGGGATCAGGC+AGG - Chr7:87912558-87912577 MsG0780041192.01.T01:CDS 60.0%
AGCCCTGCCTGATCCCCTGA+TGG + Chr7:87912564-87912583 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87912225 87912719 87912225 ID=MsG0780041192.01;Name=MsG0780041192.01
Chr7 mRNA 87912225 87912719 87912225 ID=MsG0780041192.01.T01;Parent=MsG0780041192.01;Name=MsG0780041192.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|164
Chr7 exon 87912225 87912719 87912225 ID=MsG0780041192.01.T01:exon:3295;Parent=MsG0780041192.01.T01
Chr7 CDS 87912225 87912719 87912225 ID=MsG0780041192.01.T01:cds;Parent=MsG0780041192.01.T01
Gene Sequence

>MsG0780041192.01.T01

ATGAGAGATTTTGTTGAGTACTGGTTTGATGCATCGGAAAGGATTGTCAAAGTACTTGAACTTGAACAAGGAAGTTCTACAATTGAAGTTGTCGAGATAAAGTTGCGAGCCATTGAGGTAACATCAAAGGTTTTAGAGGCAATTGCTTATGGTACTGTTATACTGCCAACTGCGAAACGTCTTGAGGTTTTGAAATTGTGGCTTCCATTTGTGAGGGTTACCAAACCAGTGATTGATTCTGCCATGACGAATTGTGAGGATGCTGCGTTGCTTAAAATGGATGGCGAAATGTGGCAATCTCTGGAATCCAGTTTTGTCTCTATCATTCTTGCATTGCCATCAGGGGATCAGGCAGGGCTTTTAACTGAATGGTTGGAAAACGAGAATATACGGTATCCAGACCTGACTGAGGCATTCGAAGTATGGTGTTATAGATCCAAGGTTGCAAGGAGAAGACTCTCATTGTTAGAGGATGAGCATGTCATGACACAGTAA

Protein sequence

>MsG0780041192.01.T01

MRDFVEYWFDASERIVKVLELEQGSSTIEVVEIKLRAIEVTSKVLEAIAYGTVILPTAKRLEVLKLWLPFVRVTKPVIDSAMTNCEDAALLKMDGEMWQSLESSFVSIILALPSGDQAGLLTEWLENENIRYPDLTEAFEVWCYRSKVARRRLSLLEDEHVMTQ*