AlfalfaGEDB | MsG0580029927.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029927.01

KEGG: K12559
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029927.01.T01	XP_024639804.1	53.153	111	39	4	1	100	40	148	1.71E-17	87.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029927.01.T01	G7K4J5	53.153	111	39	4	1	100	40	148	1.00e-17	87.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000555.01	MsG0580029927.01	0.808020	3.802295e-50	2.150253e-47
MsG0180000741.01	MsG0580029927.01	0.827671	1.375608e-54	1.336976e-51
MsG0180000821.01	MsG0580029927.01	0.807141	5.842265e-50	3.228967e-47
MsG0180001173.01	MsG0580029927.01	0.814535	1.466651e-51	9.867082e-49
MsG0180001566.01	MsG0580029927.01	0.814376	1.590008e-51	1.065103e-48
MsG0180001662.01	MsG0580029927.01	0.815386	9.492403e-52	6.535286e-49
MsG0180003031.01	MsG0580029927.01	0.804446	2.151844e-49	1.109707e-46
MsG0180003444.01	MsG0580029927.01	0.800756	1.241081e-48	5.825491e-46
MsG0180003807.01	MsG0580029927.01	0.831524	1.589747e-55	1.729646e-52
MsG0180004008.01	MsG0580029927.01	0.832538	8.930531e-56	1.001164e-52
MsG0180004437.01	MsG0580029927.01	0.805020	1.633236e-49	8.548192e-47
MsG0180004486.01	MsG0580029927.01	0.808062	3.724450e-50	2.108540e-47
MsG0180005233.01	MsG0580029927.01	0.800374	1.485094e-48	6.904032e-46
MsG0180005443.01	MsG0580029927.01	0.820682	6.028039e-53	4.801781e-50
MsG0180005445.01	MsG0580029927.01	0.840527	8.242557e-58	1.177365e-54
MsG0180005451.01	MsG0580029927.01	0.826715	2.329347e-54	2.201779e-51
MsG0280006367.01	MsG0580029927.01	0.820799	5.667473e-53	4.529482e-50
MsG0280010816.01	MsG0580029927.01	0.814650	1.383088e-51	9.333656e-49
MsG0280011293.01	MsG0580029927.01	0.823088	1.672049e-53	1.425614e-50
MsG0280011334.01	MsG0580029927.01	0.831077	2.048057e-55	2.198844e-52
MsG0280011444.01	MsG0580029927.01	0.800386	1.477003e-48	6.868290e-46
MsG0380012034.01	MsG0580029927.01	0.805300	1.426506e-49	7.520258e-47
MsG0380012067.01	MsG0580029927.01	0.809246	2.079943e-50	1.215015e-47
MsG0380013363.01	MsG0580029927.01	0.808793	2.601418e-50	1.501223e-47
MsG0480018640.01	MsG0580029927.01	0.808233	3.425284e-50	1.948000e-47
MsG0480019970.01	MsG0580029927.01	0.821985	3.017613e-53	2.493196e-50
MsG0480020242.01	MsG0580029927.01	0.804624	1.975137e-49	1.023265e-46
MsG0480020331.01	MsG0580029927.01	0.803924	2.763207e-49	1.406130e-46
MsG0480020540.01	MsG0580029927.01	0.810250	1.265317e-50	7.589664e-48
MsG0480021900.01	MsG0580029927.01	0.814058	1.868893e-51	1.241160e-48
MsG0480022094.01	MsG0580029927.01	0.804886	1.742153e-49	9.087951e-47
MsG0480022115.01	MsG0580029927.01	0.829112	6.174526e-55	6.259246e-52
MsG0480022318.01	MsG0580029927.01	0.817873	2.631510e-52	1.939380e-49
MsG0480023172.01	MsG0580029927.01	0.801547	8.550822e-49	4.094785e-46
MsG0480023377.01	MsG0580029927.01	0.810092	1.368936e-50	8.176962e-48
MsG0480023659.01	MsG0580029927.01	0.807905	4.022261e-50	2.267599e-47
MsG0480023670.01	MsG0580029927.01	0.802438	5.611331e-49	2.748714e-46
MsG0480023873.01	MsG0580029927.01	0.825021	5.882536e-54	5.298876e-51
MsG0580024157.01	MsG0580029927.01	0.830067	3.617203e-55	3.770239e-52
MsG0580024418.01	MsG0580029927.01	0.809771	1.604715e-50	9.505972e-48
MsG0580024421.01	MsG0580029927.01	0.802951	4.397557e-49	2.182411e-46
MsG0580024781.01	MsG0580029927.01	0.808929	2.432176e-50	1.408629e-47
MsG0580025008.01	MsG0580029927.01	0.813694	2.248604e-51	1.478690e-48
MsG0580025025.01	MsG0580029927.01	0.831196	1.914645e-55	2.062764e-52
MsG0380014949.01	MsG0580029927.01	0.804627	1.972767e-49	1.022107e-46
MsG0380015702.01	MsG0580029927.01	0.825155	5.470062e-54	4.946183e-51
MsG0380015980.01	MsG0580029927.01	0.811912	5.522699e-51	3.461575e-48
MsG0380016236.01	MsG0580029927.01	0.803077	4.140613e-49	2.061451e-46
MsG0380016905.01	MsG0580029927.01	0.820434	6.873016e-53	5.437803e-50
MsG0380017232.01	MsG0580029927.01	0.807909	4.013660e-50	2.263045e-47
MsG0380017375.01	MsG0580029927.01	0.815671	8.204134e-52	5.692737e-49
MsG0580029327.01	MsG0580029927.01	0.802570	5.269646e-49	2.589931e-46
MsG0580029444.01	MsG0580029927.01	0.814080	1.848560e-51	1.228366e-48
MsG0580029478.01	MsG0580029927.01	0.805447	1.328827e-49	7.031883e-47
MsG0580029704.01	MsG0580029927.01	0.807176	5.742739e-50	3.176935e-47
MsG0580029926.01	MsG0580029927.01	0.824904	6.269972e-54	5.629083e-51
MsG0580029927.01	MsG0680030364.01	0.800930	1.143603e-48	5.392067e-46
MsG0580029927.01	MsG0680033392.01	0.806315	8.730354e-50	4.723741e-47
MsG0580029927.01	MsG0680034757.01	0.823725	1.186410e-53	1.030044e-50
MsG0580029927.01	MsG0680035040.01	0.801301	9.602748e-49	4.570246e-46
MsG0580029927.01	MsG0680035764.01	0.804110	2.528450e-49	1.292733e-46
MsG0580029927.01	MsG0780036018.01	0.803681	3.103678e-49	1.569413e-46
MsG0580029927.01	MsG0780036430.01	0.800850	1.187722e-48	5.588637e-46
MsG0580029927.01	MsG0780036773.01	0.861730	8.356557e-64	2.387303e-60
MsG0580029927.01	MsG0780036789.01	0.835395	1.720459e-56	2.101263e-53
MsG0580029927.01	MsG0780038051.01	0.822897	1.852173e-53	1.570627e-50
MsG0580029927.01	MsG0780038443.01	0.808141	3.583144e-50	2.032861e-47
MsG0580029927.01	MsG0780039655.01	0.815368	9.582975e-52	6.594344e-49
MsG0580029927.01	MsG0780039717.01	0.816349	5.788442e-52	4.091834e-49
MsG0580029927.01	MsG0780039777.01	0.801753	7.758646e-49	3.734762e-46
MsG0580029927.01	MsG0780039858.01	0.807555	4.773694e-50	2.666921e-47
MsG0580029927.01	MsG0780039864.01	0.800484	1.410411e-48	6.574874e-46
MsG0580029927.01	MsG0780040044.01	0.810460	1.140090e-50	6.876149e-48
MsG0580029927.01	MsG0780040176.01	0.800756	1.241454e-48	5.827170e-46
MsG0580029927.01	MsG0780040664.01	0.816950	4.245100e-52	3.050756e-49
MsG0580029927.01	MsG0780041033.01	0.804546	2.051215e-49	1.060520e-46
MsG0580029927.01	MsG0780041194.01	0.812076	5.085529e-51	3.201988e-48
MsG0580029927.01	MsG0780041305.01	0.801732	7.838523e-49	3.771167e-46
MsG0580029927.01	MsG0780041390.01	0.802814	4.693620e-49	2.321428e-46
MsG0580029927.01	MsG0880042939.01	0.813917	2.007398e-51	1.328042e-48
MsG0580029927.01	MsG0880043480.01	0.822361	2.468434e-53	2.061676e-50
MsG0580029927.01	MsG0880043522.01	0.803345	3.644280e-49	1.826884e-46
MsG0580029927.01	MsG0880044732.01	0.803457	3.453770e-49	1.736467e-46
MsG0580029927.01	MsG0880046027.01	0.828175	1.040206e-54	1.026079e-51
MsG0280007710.01	MsG0580029927.01	0.814061	1.865860e-51	1.239264e-48
MsG0280007764.01	MsG0580029927.01	0.849423	3.264294e-60	6.184821e-57
MsG0280007984.01	MsG0580029927.01	0.810805	9.600683e-51	5.844179e-48
MsG0280008030.01	MsG0580029927.01	0.808800	2.591934e-50	1.496039e-47
MsG0280009748.01	MsG0580029927.01	0.813075	3.074586e-51	1.988029e-48
MsG0280009996.01	MsG0580029927.01	0.806946	6.425655e-50	3.533478e-47
MsG0280010109.01	MsG0580029927.01	0.826169	3.143218e-54	2.925299e-51
MsG0280010447.01	MsG0580029927.01	0.803482	3.413947e-49	1.717553e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029927.01.T01	MTR_5g095050	53.153	111	39	4	1	100	40	148	2.54e-21	87.4
MsG0580029927.01.T01	MTR_5g094950	46.591	88	35	2	6	82	36	122	2.06e-15	70.5
MsG0580029927.01.T01	MTR_5g094960	48.000	75	32	1	11	85	34	101	3.10e-13	64.3
MsG0580029927.01.T01	MTR_5g095390	40.404	99	43	3	2	89	26	119	6.27e-13	63.2
MsG0580029927.01.T01	MTR_5g094850	38.947	95	45	2	2	85	25	117	1.09e-12	62.8
MsG0580029927.01.T01	MTR_5g094850	38.947	95	45	2	2	85	25	117	1.33e-12	62.4
MsG0580029927.01.T01	MTR_5g095370	42.353	85	47	2	1	85	22	104	4.43e-12	60.1
MsG0580029927.01.T01	MTR_5g094990	41.667	84	40	2	2	85	28	102	2.10e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 8 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGATCAAATTTGTCAGAAA+TGG	0.314011	5:-104045361	MsG0580029927.01.T01:CDS
TGATAAGGTTGAAGCTGTTT+TGG	0.344511	5:-104045486	MsG0580029927.01.T01:CDS
TTGATGAGTTTCTTGCAATT+TGG	0.348899	5:+104045337	None:intergenic
AAAAGATCTTTGGAAGCTAA+TGG	0.352670	5:-104045285	MsG0580029927.01.T01:CDS
TTCAAAATGAGAAAATTTCA+AGG	0.437344	5:-104045412	MsG0580029927.01.T01:CDS
AATTTCAAGGTTACATGTAG+AGG	0.548212	5:-104045399	MsG0580029927.01.T01:CDS
CACGTTGAACAAGATCATCA+TGG	0.563594	5:+104045540	None:intergenic
TCGTGCTGAAATCTATGATA+AGG	0.576606	5:-104045501	MsG0580029927.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAAAATGAGAAAATTTCA+AGG	-	Chr5:104045404-104045423	MsG0580029927.01.T01:CDS	20.0%
	AATTTCAAGGTTACATGTAG+AGG	-	Chr5:104045417-104045436	MsG0580029927.01.T01:CDS	30.0%
	GAGATCAAATTTGTCAGAAA+TGG	-	Chr5:104045455-104045474	MsG0580029927.01.T01:CDS	30.0%
	TTGATGAGTTTCTTGCAATT+TGG	+	Chr5:104045482-104045501	None:intergenic	30.0%
!	AAAAGATCTTTGGAAGCTAA+TGG	-	Chr5:104045531-104045550	MsG0580029927.01.T01:CDS	30.0%
!!	TCTTTTGAAAGATTCCAAGA+AGG	-	Chr5:104045504-104045523	MsG0580029927.01.T01:CDS	30.0%
	AGAAGGCTCAAAAAGATCTT+TGG	-	Chr5:104045521-104045540	MsG0580029927.01.T01:CDS	35.0%
	TCGTGCTGAAATCTATGATA+AGG	-	Chr5:104045315-104045334	MsG0580029927.01.T01:CDS	35.0%
!	TGATAAGGTTGAAGCTGTTT+TGG	-	Chr5:104045330-104045349	MsG0580029927.01.T01:CDS	35.0%
!!	AAGATCTTTTTGAGCCTTCT+TGG	+	Chr5:104045521-104045540	None:intergenic	35.0%
!!!	CATTTTGAAGCTCATGTTGA+AGG	+	Chr5:104045393-104045412	None:intergenic	35.0%
	CACGTTGAACAAGATCATCA+TGG	+	Chr5:104045279-104045298	None:intergenic	40.0%
!	CTTGTTCAACGTGTTTTGCT+TGG	-	Chr5:104045286-104045305	MsG0580029927.01.T01:CDS	40.0%
!!!	TGAAGCTGTTTTGGTGACAA+GGG	-	Chr5:104045339-104045358	MsG0580029927.01.T01:CDS	40.0%
!!!	TGTTTTGGTGACAAGGGATT+TGG	-	Chr5:104045345-104045364	MsG0580029927.01.T01:CDS	40.0%
!!!	TTGAAGCTGTTTTGGTGACA+AGG	-	Chr5:104045338-104045357	MsG0580029927.01.T01:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	104045268	104045570	104045268	ID=MsG0580029927.01;Name=MsG0580029927.01
Chr5	mRNA	104045268	104045570	104045268	ID=MsG0580029927.01.T01;Parent=MsG0580029927.01;Name=MsG0580029927.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|100
Chr5	exon	104045268	104045570	104045268	ID=MsG0580029927.01.T01:exon:35337;Parent=MsG0580029927.01.T01
Chr5	CDS	104045268	104045570	104045268	ID=MsG0580029927.01.T01:cds;Parent=MsG0580029927.01.T01

Gene Sequence	>MsG0580029927.01.T01 ATGACTATCCATGATGATCTTGTTCAACGTGTTTTGCTTGGTGCTTGTCGTGCTGAAATCTATGATAAGGTTGAAGCTGTTTTGGTGACAAGGGATTTGGATCTCAAACAACAGATTCAACTCCTTCAACATGAGCTTCAAAATGAGAAAATTTCAAGGTTACATGTAGAGGAAGAGCTTAACAAAAGAGATCAAATTTGTCAGAAATGGACCAAATTGCAAGAAACTCATCAAGCTCTTTTGAAAGATTCCAAGAAGGCTCAAAAAGATCTTTGGAAGCTAATGGAAACATTGATCTTCTAA
Protein sequence	>MsG0580029927.01.T01 MTIHDDLVQRVLLGACRAEIYDKVEAVLVTRDLDLKQQIQLLQHELQNEKISRLHVEEELNKRDQICQKWTKLQETHQALLKDSKKAQKDLWKLMETLIF*