AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022142.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022142.01.T01 MTR_4g084750 98.276 116 2 0 1 116 1 116 2.01e-79 232
MsG0480022142.01.T01 MTR_5g014890 72.308 65 15 1 52 116 113 174 1.74e-23 91.7
MsG0480022142.01.T01 MTR_4g100550 55.556 90 37 2 29 116 83 171 3.11e-22 88.6
MsG0480022142.01.T01 MTR_2g061030 67.188 64 21 0 53 116 188 251 4.24e-22 89.0
MsG0480022142.01.T01 MTR_5g013010 53.922 102 36 3 21 116 91 187 7.05e-22 85.9
MsG0480022142.01.T01 MTR_7g093430 75.862 58 13 1 59 116 155 211 1.64e-21 86.7
MsG0480022142.01.T01 MTR_8g006705 77.358 53 12 0 64 116 145 197 2.04e-21 86.7
MsG0480022142.01.T01 MTR_1g017090 54.000 100 41 3 20 116 84 181 2.09e-21 86.3
MsG0480022142.01.T01 MTR_4g097600 63.636 66 21 1 51 116 116 178 4.24e-21 85.5
MsG0480022142.01.T01 MTR_5g013010 72.414 58 16 0 59 116 130 187 6.67e-21 85.1
MsG0480022142.01.T01 MTR_1g054285 66.667 66 21 1 51 116 141 205 2.91e-20 83.6
MsG0480022142.01.T01 MTR_3g103590 67.797 59 19 0 58 116 138 196 1.85e-19 81.3
MsG0480022142.01.T01 MTR_2g038625 52.632 76 32 2 44 116 59 133 2.77e-16 71.6
MsG0480022142.01.T01 MTR_2g038580 52.632 76 32 2 44 116 59 133 2.77e-16 71.6
MsG0480022142.01.T01 MTR_4g126900 68.000 50 16 0 67 116 90 139 1.32e-15 70.1
MsG0480022142.01.T01 MTR_6g007647 60.000 55 22 0 62 116 30 84 1.62e-15 70.1
MsG0480022142.01.T01 MTR_6g007647 60.000 55 22 0 62 116 64 118 2.59e-15 70.1
MsG0480022142.01.T01 MTR_8g469430 57.407 54 23 0 63 116 87 140 4.48e-13 63.9
MsG0480022142.01.T01 MTR_5g038280 38.211 123 61 3 4 116 21 138 5.08e-13 63.9
MsG0480022142.01.T01 MTR_5g019650 57.692 52 22 0 65 116 53 104 1.53e-12 61.6
MsG0480022142.01.T01 MTR_5g019680 57.692 52 22 0 65 116 53 104 1.72e-12 61.6
MsG0480022142.01.T01 MTR_8g468210 37.500 112 65 2 5 116 22 128 1.32e-11 59.7
MsG0480022142.01.T01 MTR_3g086790 51.515 66 30 1 53 116 46 111 1.49e-11 59.7
MsG0480022142.01.T01 MTR_4g107650 53.846 52 24 0 65 116 95 146 2.10e-11 59.7
MsG0480022142.01.T01 MTR_3g080100 40.404 99 51 3 26 116 12 110 2.12e-11 58.9
MsG0480022142.01.T01 MTR_3g080100 48.485 66 32 1 53 116 6 71 5.41e-11 57.4
MsG0480022142.01.T01 MTR_5g039000 49.231 65 33 0 52 116 64 128 9.57e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022142.01.T01 AT2G01430 60.833 120 40 3 1 116 74 190 3.91e-42 138
MsG0480022142.01.T01 AT2G01430 60.833 120 40 3 1 116 74 190 7.34e-41 136
MsG0480022142.01.T01 AT1G70920 55.738 122 46 4 1 116 1 120 5.00e-25 94.4
MsG0480022142.01.T01 AT1G70920 58.889 90 31 2 33 116 1 90 3.80e-24 91.3
MsG0480022142.01.T01 AT2G22800 71.212 66 15 1 51 116 103 164 2.76e-23 91.3
MsG0480022142.01.T01 AT5G06710 48.305 118 50 4 1 116 133 241 1.06e-22 89.7
MsG0480022142.01.T01 AT5G06710 48.305 118 50 4 1 116 133 241 5.67e-22 88.6
MsG0480022142.01.T01 AT4G37790 73.684 57 15 0 60 116 121 177 6.26e-22 87.8
MsG0480022142.01.T01 AT3G60390 66.667 66 21 1 52 116 148 213 8.30e-22 87.8
MsG0480022142.01.T01 AT4G16780 70.690 58 17 0 59 116 123 180 8.98e-22 87.4
MsG0480022142.01.T01 AT4G17460 62.500 64 24 0 53 116 123 186 1.18e-20 84.3
MsG0480022142.01.T01 AT5G47370 63.636 66 21 1 51 116 119 181 4.43e-20 82.8
MsG0480022142.01.T01 AT2G44910 71.698 53 15 0 64 116 162 214 2.39e-18 78.6
MsG0480022142.01.T01 AT3G01470 62.963 54 20 0 63 116 66 119 7.14e-15 68.6
MsG0480022142.01.T01 AT4G36740 50.820 61 30 0 56 116 46 106 1.88e-12 61.6
MsG0480022142.01.T01 AT4G36740 50.820 61 30 0 56 116 47 107 1.88e-12 61.6
MsG0480022142.01.T01 AT5G66700 48.529 68 33 1 49 116 57 122 5.74e-12 60.5
MsG0480022142.01.T01 AT2G18550 49.206 63 32 0 54 116 50 112 6.14e-12 60.1

Find 36 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAAGAAAGCAGCGTTAA+TGG 0.245343 4:-68818474 MsG0480022142.01.T01:CDS
GATGAGGGGAGAAAAGATAT+TGG 0.398648 4:+68819465 None:intergenic
TGCCATCCATTCTTCTTCTA+CGG 0.429731 4:+68818506 None:intergenic
TGAAGCTTTCTTCAAGGAGA+TGG 0.431949 4:+68818409 None:intergenic
ACAGAAGCAGAAAGAGTGTT+TGG 0.439755 4:-68818199 MsG0480022142.01.T01:intron
ATTGGTGAAGAAGCAAAGCC+TGG 0.460244 4:+68819483 None:intergenic
ACCTACAAGTTCCTCCAGCT+TGG 0.470781 4:-68819523 MsG0480022142.01.T01:CDS
AACCACACCTCCACTTGCCT+TGG 0.476841 4:+68818156 None:intergenic
GAAGCTTTCTTCAAGGAGAT+GGG 0.480176 4:+68818410 None:intergenic
ATTCTTCTTCTACGGTTACT+CGG 0.502354 4:+68818514 None:intergenic
TAATTACTGGGTTTAAAGTG+TGG 0.513978 4:+68818379 None:intergenic
TCCAAGCTGGAGGAACTTGT+AGG 0.518571 4:+68819522 None:intergenic
GAGTAACCGTAGAAGAAGAA+TGG 0.519061 4:-68818512 MsG0480022142.01.T01:CDS
GGTTTCAAAACCGTAGGGCC+AGG 0.531848 4:-68818137 MsG0480022142.01.T01:CDS
AGCAAAGCCTGGGATAGATA+TGG 0.534631 4:+68819494 None:intergenic
AAAACCGTAGGGCCAGGTAA+AGG 0.559384 4:-68818131 None:intergenic
GAATTGACCATATCTATCCC+AGG 0.565626 4:-68819501 MsG0480022142.01.T01:CDS
ATAGAGGATTAGCCTTTACC+TGG 0.573524 4:+68818119 None:intergenic
AGAATGGATGGCACTAGAAG+AGG 0.577292 4:-68818496 MsG0480022142.01.T01:CDS
TTAGCCTTTACCTGGCCCTA+CGG 0.577524 4:+68818127 None:intergenic
AGATATGGTCAATTCCAAGC+TGG 0.579606 4:+68819509 None:intergenic
ACACCTCCACTTGCCTTGGT+CGG 0.580254 4:+68818160 None:intergenic
GTGCTACTTACCAGATGATG+AGG 0.582615 4:+68819449 None:intergenic
TGCAGCTTAAGCTCCGACCA+AGG 0.586714 4:-68818173 MsG0480022142.01.T01:CDS
GACCAAGGCAAGTGGAGGTG+TGG 0.601638 4:-68818158 MsG0480022142.01.T01:CDS
AACCGTAGAAGAAGAATGGA+TGG 0.604290 4:-68818508 MsG0480022142.01.T01:CDS
TGCTACTTACCAGATGATGA+GGG 0.609343 4:+68819450 None:intergenic
TAAGCTCCGACCAAGGCAAG+TGG 0.615426 4:-68818166 MsG0480022142.01.T01:CDS
AGGTGTGGTTTCAAAACCGT+AGG 0.616349 4:-68818143 MsG0480022142.01.T01:CDS
CAAGAAACTGCGTCTAACAA+AGG 0.630784 4:-68818439 MsG0480022142.01.T01:CDS
TTGGTGAAGAAGCAAAGCCT+GGG 0.631749 4:+68819484 None:intergenic
TATGGTCAATTCCAAGCTGG+AGG 0.639066 4:+68819512 None:intergenic
GTTAGACGCAGTTTCTTGCG+AGG 0.647544 4:+68818444 None:intergenic
GGTGTGGTTTCAAAACCGTA+GGG 0.647806 4:-68818142 MsG0480022142.01.T01:CDS
GCTCCGACCAAGGCAAGTGG+AGG 0.651935 4:-68818163 MsG0480022142.01.T01:CDS
GCTACTTACCAGATGATGAG+GGG 0.700104 4:+68819451 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAATAATAAATTTAA+GGG + Chr4:68818489-68818508 None:intergenic 0.0%
!! TATAAAAAATAATAAATTTA+AGG + Chr4:68818490-68818509 None:intergenic 0.0%
!! AAAAATTCATAATTCTTTAT+AGG + Chr4:68819093-68819112 None:intergenic 10.0%
!! AAAGTAAAAAATTGTATATT+AGG + Chr4:68818636-68818655 None:intergenic 10.0%
!! AACAAAAAGTTAATATTAAA+CGG - Chr4:68818509-68818528 MsG0480022142.01.T01:CDS 10.0%
!! AAGAAAATATGAATAAATTA+AGG + Chr4:68819356-68819375 None:intergenic 10.0%
!! AATAAAATTAAAACAATGAT+CGG - Chr4:68818425-68818444 MsG0480022142.01.T01:CDS 10.0%
!! AATTATATGCATATTAATTA+AGG + Chr4:68819438-68819457 None:intergenic 10.0%
!! TATATTATATATCTAACTTT+AGG + Chr4:68819057-68819076 None:intergenic 10.0%
!! TTAATTAATATGCATATAAT+TGG - Chr4:68819437-68819456 MsG0480022142.01.T01:intron 10.0%
!! TGAAAAAATGATATATTTGT+CGG - Chr4:68818953-68818972 MsG0480022142.01.T01:intron 15.0%
!! TTAAAAAATGAAACCAATAA+TGG + Chr4:68818381-68818400 None:intergenic 15.0%
!!! AAAGAATTATGAATTTTTGT+TGG - Chr4:68819095-68819114 MsG0480022142.01.T01:intron 15.0%
!!! AAGAATTATGAATTTTTGTT+GGG - Chr4:68819096-68819115 MsG0480022142.01.T01:intron 15.0%
!!! TTCATTTATTTTACCATTAT+TGG - Chr4:68818365-68818384 MsG0480022142.01.T01:intron 15.0%
!!! TTGATCTTTATTGTTTTTTT+AGG - Chr4:68818702-68818721 MsG0480022142.01.T01:intron 15.0%
!! ATAAATTAAGGTCATGTAAA+TGG + Chr4:68819344-68819363 None:intergenic 20.0%
!! GAAAAAATGATATATTTGTC+GGG - Chr4:68818954-68818973 MsG0480022142.01.T01:intron 20.0%
!! TAAATTAAGGTCATGTAAAT+GGG + Chr4:68819343-68819362 None:intergenic 20.0%
!! TACAATGACTGAAAAAAATA+AGG + Chr4:68818663-68818682 None:intergenic 20.0%
! AAAAAACTTTAGGTACTAGT+TGG + Chr4:68819017-68819036 None:intergenic 25.0%
! AATATTAAACGGCCTATAAA+GGG - Chr4:68818520-68818539 MsG0480022142.01.T01:CDS 25.0%
! AGAAGAAGAAAATAAGTATG+TGG + Chr4:68818549-68818568 None:intergenic 25.0%
! ATAAAAAAACACAACTGTAG+TGG + Chr4:68818466-68818485 None:intergenic 25.0%
! ATTTCACCTATATTTGCTTT+AGG + Chr4:68818605-68818624 None:intergenic 25.0%
! GAAGAAGAAAATAAGTATGT+GGG + Chr4:68818548-68818567 None:intergenic 25.0%
! TAACTTCCTAAAGCAAATAT+AGG - Chr4:68818596-68818615 MsG0480022142.01.T01:intron 25.0%
! TAATATTAAACGGCCTATAA+AGG - Chr4:68818519-68818538 MsG0480022142.01.T01:CDS 25.0%
! TCCTCTCAAAAAAAAACTTT+AGG + Chr4:68819027-68819046 None:intergenic 25.0%
!! ATGAATTTTTGTTGGGAAAT+TGG - Chr4:68819103-68819122 MsG0480022142.01.T01:intron 25.0%
!! TCTTGGTAAAAGAAAGATTT+TGG + Chr4:68819320-68819339 None:intergenic 25.0%
!! TGAATTTTTGTTGGGAAATT+GGG - Chr4:68819104-68819123 MsG0480022142.01.T01:intron 25.0%
!! TTATTTTCTTCTTCTTCTTG+AGG - Chr4:68818554-68818573 MsG0480022142.01.T01:intron 25.0%
!!! ACCTAAAGTTTTTTTTTGAG+AGG - Chr4:68819023-68819042 MsG0480022142.01.T01:intron 25.0%
!!! ATTTTGGAGGTATAATTACT+GGG + Chr4:68819304-68819323 None:intergenic 25.0%
ATACTAGACAAAGATGCAAA+AGG + Chr4:68818259-68818278 None:intergenic 30.0%
CGTGCAAAATTAATTCATGT+AGG + Chr4:68818851-68818870 None:intergenic 30.0%
TAGAAATATAGCATCTCTCA+TGG + Chr4:68819407-68819426 None:intergenic 30.0%
! TAATTACTGGGTTTAAAGTG+TGG + Chr4:68819292-68819311 None:intergenic 30.0%
! TGGTAAAAGAAAGATTTTGG+AGG + Chr4:68819317-68819336 None:intergenic 30.0%
!! GATTTTGGAGGTATAATTAC+TGG + Chr4:68819305-68819324 None:intergenic 30.0%
!!! TTTTTCTGAAGCTTTCTTCA+AGG + Chr4:68819268-68819287 None:intergenic 30.0%
AAAGACACAAACATGAAGAG+AGG + Chr4:68818319-68818338 None:intergenic 35.0%
AAGACACAAACATGAAGAGA+GGG + Chr4:68818318-68818337 None:intergenic 35.0%
ATTCTTCTTCTACGGTTACT+CGG + Chr4:68819157-68819176 None:intergenic 35.0%
AACCGTAGAAGAAGAATGGA+TGG - Chr4:68819160-68819179 MsG0480022142.01.T01:intron 40.0%
AAGGTCATGTAAATGGGTCT+TGG + Chr4:68819337-68819356 None:intergenic 40.0%
AGATATGGTCAATTCCAAGC+TGG + Chr4:68818162-68818181 None:intergenic 40.0%
AGTATGTGGGAACCCTTTAT+AGG + Chr4:68818535-68818554 None:intergenic 40.0%
CAAGAAACTGCGTCTAACAA+AGG - Chr4:68819229-68819248 MsG0480022142.01.T01:intron 40.0%
GAAGAAGAAAGCAGCGTTAA+TGG - Chr4:68819194-68819213 MsG0480022142.01.T01:intron 40.0%
GAAGCTTTCTTCAAGGAGAT+GGG + Chr4:68819261-68819280 None:intergenic 40.0%
GAATTGACCATATCTATCCC+AGG - Chr4:68818167-68818186 MsG0480022142.01.T01:CDS 40.0%
GAGTAACCGTAGAAGAAGAA+TGG - Chr4:68819156-68819175 MsG0480022142.01.T01:intron 40.0%
GATGAGGGGAGAAAAGATAT+TGG + Chr4:68818206-68818225 None:intergenic 40.0%
TATAGCATCTCTCATGGACA+TGG + Chr4:68819401-68819420 None:intergenic 40.0%
TGAAGCTTTCTTCAAGGAGA+TGG + Chr4:68819262-68819281 None:intergenic 40.0%
TGCCATCCATTCTTCTTCTA+CGG + Chr4:68819165-68819184 None:intergenic 40.0%
! GGGTGTTACAGTGAAAAAGT+TGG - Chr4:68819124-68819143 MsG0480022142.01.T01:intron 40.0%
! TGCTACTTACCAGATGATGA+GGG + Chr4:68818221-68818240 None:intergenic 40.0%
!! ACAGAAGCAGAAAGAGTGTT+TGG - Chr4:68819469-68819488 MsG0480022142.01.T01:CDS 40.0%
AGAATGGATGGCACTAGAAG+AGG - Chr4:68819172-68819191 MsG0480022142.01.T01:intron 45.0%
AGCAAAGCCTGGGATAGATA+TGG + Chr4:68818177-68818196 None:intergenic 45.0%
AGGTGTGGTTTCAAAACCGT+AGG - Chr4:68819525-68819544 MsG0480022142.01.T01:CDS 45.0%
ATTGGTGAAGAAGCAAAGCC+TGG + Chr4:68818188-68818207 None:intergenic 45.0%
GCTACTTACCAGATGATGAG+GGG + Chr4:68818220-68818239 None:intergenic 45.0%
GGTGTGGTTTCAAAACCGTA+GGG - Chr4:68819526-68819545 MsG0480022142.01.T01:CDS 45.0%
TATGGTCAATTCCAAGCTGG+AGG + Chr4:68818159-68818178 None:intergenic 45.0%
TTGGTGAAGAAGCAAAGCCT+GGG + Chr4:68818187-68818206 None:intergenic 45.0%
! TCTTTTCTCCCCTCATCATC+TGG - Chr4:68818209-68818228 MsG0480022142.01.T01:intron 45.0%
!! GTGCTACTTACCAGATGATG+AGG + Chr4:68818222-68818241 None:intergenic 45.0%
ACCTACAAGTTCCTCCAGCT+TGG - Chr4:68818145-68818164 MsG0480022142.01.T01:CDS 50.0%
GTTAGACGCAGTTTCTTGCG+AGG + Chr4:68819227-68819246 None:intergenic 50.0%
TCCAAGCTGGAGGAACTTGT+AGG + Chr4:68818149-68818168 None:intergenic 50.0%
AACCACACCTCCACTTGCCT+TGG + Chr4:68819515-68819534 None:intergenic 55.0%
ACACCTCCACTTGCCTTGGT+CGG + Chr4:68819511-68819530 None:intergenic 55.0%
GGTTTCAAAACCGTAGGGCC+AGG - Chr4:68819531-68819550 MsG0480022142.01.T01:CDS 55.0%
TAAGCTCCGACCAAGGCAAG+TGG - Chr4:68819502-68819521 MsG0480022142.01.T01:CDS 55.0%
TGCAGCTTAAGCTCCGACCA+AGG - Chr4:68819495-68819514 MsG0480022142.01.T01:CDS 55.0%
GACCAAGGCAAGTGGAGGTG+TGG - Chr4:68819510-68819529 MsG0480022142.01.T01:CDS 60.0%
GCTCCGACCAAGGCAAGTGG+AGG - Chr4:68819505-68819524 MsG0480022142.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 68818134 68819556 68818134 ID=MsG0480022142.01;Name=MsG0480022142.01
Chr4 mRNA 68818134 68819556 68818134 ID=MsG0480022142.01.T01;Parent=MsG0480022142.01;Name=MsG0480022142.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|3|0|116
Chr4 exon 68819460 68819556 68819460 ID=MsG0480022142.01.T01:exon:18943;Parent=MsG0480022142.01.T01
Chr4 exon 68818386 68818555 68818386 ID=MsG0480022142.01.T01:exon:18942;Parent=MsG0480022142.01.T01
Chr4 exon 68818134 68818217 68818134 ID=MsG0480022142.01.T01:exon:18941;Parent=MsG0480022142.01.T01
Chr4 CDS 68819460 68819556 68819460 ID=MsG0480022142.01.T01:cds;Parent=MsG0480022142.01.T01
Chr4 CDS 68818386 68818555 68818386 ID=MsG0480022142.01.T01:cds;Parent=MsG0480022142.01.T01
Chr4 CDS 68818134 68818217 68818134 ID=MsG0480022142.01.T01:cds;Parent=MsG0480022142.01.T01
Gene Sequence

>MsG0480022142.01.T01

ATGGCAGTTTTACCTACAAGTTCCTCCAGCTTGGAATTGACCATATCTATCCCAGGCTTTGCTTCTTCACCAATATCTTTTCTCCCCTCATCATCTGTGAAAAAGTTGGATGTGAACCGAGTAACCGTAGAAGAAGAATGGATGGCACTAGAAGAGGAAGAAGAAAGCAGCGTTAATGGAGACACTCCTCGCAAGAAACTGCGTCTAACAAAGGAACAGTCCCATCTCCTTGAAGAAAGCTTCAGAAAAAACCACACTTTAAACCCAAAGCAGAAAGAGTGTTTGGCAATGCAGCTTAAGCTCCGACCAAGGCAAGTGGAGGTGTGGTTTCAAAACCGTAGGGCCAGGTAA

Protein sequence

>MsG0480022142.01.T01

MAVLPTSSSSLELTISIPGFASSPISFLPSSSVKKLDVNRVTVEEEWMALEEEEESSVNGDTPRKKLRLTKEQSHLLEESFRKNHTLNPKQKECLAMQLKLRPRQVEVWFQNRRAR*