AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030182.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCAGTAAAGATAGATTA+AGG 0.317109 5:-107915372 MsG0580030182.01.T01:CDS
AGTCGAAACCCAGTAGTTAA+TGG 0.326019 5:+107915526 None:intergenic
ATTGCAATCATGCTTTCAAC+TGG 0.422930 5:-107915441 MsG0580030182.01.T01:CDS
GTCTCTGCTATACTTTATAT+CGG 0.428279 5:-107915483 MsG0580030182.01.T01:CDS
ATGCTTTCAACTGGAACCAT+TGG 0.461754 5:-107915432 MsG0580030182.01.T01:CDS
TGGAAGGCATTCTATGCATC+AGG 0.465968 5:-107915578 None:intergenic
TTTGTACTCCATTAACTACT+GGG 0.489517 5:-107915534 MsG0580030182.01.T01:CDS
TTCGACTTGCAGAGTTTGAG+TGG 0.532363 5:-107915510 MsG0580030182.01.T01:CDS
TATCGGCTATTCACTGCTCA+TGG 0.559083 5:-107915466 MsG0580030182.01.T01:CDS
ATATAAAGTATAGCAGAGAC+AGG 0.561532 5:+107915486 None:intergenic
GAACGAGACAAGGAAACCAA+TGG 0.655608 5:+107915416 None:intergenic
GCACAAAGTAGAACGAGACA+AGG 0.678415 5:+107915406 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCATCAGTAAAGATAGATTA+AGG - Chr5:107915535-107915554 MsG0580030182.01.T01:CDS 25.0%
ATATAAAGTATAGCAGAGAC+AGG + Chr5:107915424-107915443 None:intergenic 30.0%
GTCTCTGCTATACTTTATAT+CGG - Chr5:107915424-107915443 MsG0580030182.01.T01:CDS 30.0%
TTTGTACTCCATTAACTACT+GGG - Chr5:107915373-107915392 MsG0580030182.01.T01:CDS 30.0%
! TTTTGTACTCCATTAACTAC+TGG - Chr5:107915372-107915391 MsG0580030182.01.T01:CDS 30.0%
ATTGCAATCATGCTTTCAAC+TGG - Chr5:107915466-107915485 MsG0580030182.01.T01:CDS 35.0%
AGTCGAAACCCAGTAGTTAA+TGG + Chr5:107915384-107915403 None:intergenic 40.0%
ATGCTTTCAACTGGAACCAT+TGG - Chr5:107915475-107915494 MsG0580030182.01.T01:CDS 40.0%
GAACGAGACAAGGAAACCAA+TGG + Chr5:107915494-107915513 None:intergenic 45.0%
GCACAAAGTAGAACGAGACA+AGG + Chr5:107915504-107915523 None:intergenic 45.0%
! TATCGGCTATTCACTGCTCA+TGG - Chr5:107915441-107915460 MsG0580030182.01.T01:CDS 45.0%
! TTCGACTTGCAGAGTTTGAG+TGG - Chr5:107915397-107915416 MsG0580030182.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr5 gene 107915342 107915587 107915342 ID=MsG0580030182.01;Name=MsG0580030182.01
Chr5 mRNA 107915342 107915587 107915342 ID=MsG0580030182.01.T01;Parent=MsG0580030182.01;Name=MsG0580030182.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|81
Chr5 exon 107915342 107915587 107915342 ID=MsG0580030182.01.T01:exon:17597;Parent=MsG0580030182.01.T01
Chr5 CDS 107915342 107915587 107915342 ID=MsG0580030182.01.T01:cds;Parent=MsG0580030182.01.T01
Gene Sequence

>MsG0580030182.01.T01

ATGCATCAGGCTCTGTTTCTCTTTATGTCTTTTTGTACTCCATTAACTACTGGGTTTCGACTTGCAGAGTTTGAGTGGACCTGTCTCTGCTATACTTTATATCGGCTATTCACTGCTCATGGCAATTGCAATCATGCTTTCAACTGGAACCATTGGTTTCCTTGTCTCGTTCTACTTTGTGCATTACTTGTTCTCATCAGTAAAGATAGATTAAGGTTAATCTTTTTGCTATCTCTCATCAACTAA

Protein sequence

>MsG0580030182.01.T01

MHQALFLFMSFCTPLTTGFRLAEFEWTCLCYTLYRLFTAHGNCNHAFNWNHWFPCLVLLCALLVLISKDRLRLIFLLSLIN*