AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015716.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015716.01.T01 MTR_2g048385 72.028 143 38 1 1 141 63 205 5.94e-60 186
MsG0380015716.01.T01 MTR_3g075220 93.333 60 4 0 108 167 308 367 7.16e-35 125
MsG0380015716.01.T01 MTR_7g018620 41.837 98 55 2 2 99 124 219 9.15e-12 62.4
MsG0380015716.01.T01 MTR_7g451160 48.485 66 31 2 2 67 65 127 1.36e-11 61.2
MsG0380015716.01.T01 MTR_6g052002 43.878 98 53 2 2 99 64 159 6.57e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015716.01.T01 AT3G48425 70.000 60 18 0 108 167 303 362 2.55e-24 97.4

Find 42 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTCGCTTGCATTTATT+TGG 0.227826 3:-74160868 MsG0380015715.01.T01:intron
TTTCCTATCGCGGTGGGATT+TGG 0.243457 3:+74160716 None:intergenic
AACCGGTCCAATTCAGTTTC+AGG 0.270598 3:+74160791 None:intergenic
TTGCAATAAAGAAATGAGTA+TGG 0.295957 3:-74160573 MsG0380015715.01.T01:intron
GTTGTCTCCTCAGGTTTCTA+TGG 0.325287 3:-74158836 MsG0380015715.01.T01:intron
GGCATGATATCCCGATATTC+CGG 0.375562 3:+74160836 None:intergenic
GGAGAGAGCTCCAAGGTAAC+AGG 0.385509 3:+74158800 None:intergenic
AGAGGAACCTGAAACTGAAT+TGG 0.393937 3:-74160798 MsG0380015715.01.T01:intron
CCACAATCATGACATGCATA+TGG 0.397957 3:+74160636 None:intergenic
GCATGTCATGATTGTGGAAA+TGG 0.403241 3:-74160630 MsG0380015715.01.T01:intron
CGGTGGGATTTGGATTTCCC+TGG 0.428767 3:+74160726 None:intergenic
CTTACTGTGTTTCTCTTGCA+TGG 0.436471 3:-74158677 MsG0380015715.01.T01:exon
ACATTAATAAGTACTTATAC+TGG 0.437619 3:+74158752 None:intergenic
AGGTTTGGAAGATGTAGAAA+TGG 0.438125 3:-74160687 MsG0380015715.01.T01:intron
GGTTTGGAAGATGTAGAAAT+GGG 0.438460 3:-74160686 MsG0380015715.01.T01:intron
TTTGAAGGTACCGCGGAAAA+CGG 0.439053 3:-74159876 MsG0380015715.01.T01:intron
TGATCATTGTCCTGTTACCT+TGG 0.459781 3:-74158810 MsG0380015715.01.T01:CDS
TGCAATAAAGAAATGAGTAT+GGG 0.478957 3:-74160572 MsG0380015715.01.T01:intron
CATGATTGTGGAAATGGAAC+AGG 0.481577 3:-74160624 MsG0380015715.01.T01:intron
GCATGTGAAATGCATGGAAA+AGG 0.488169 3:-74159802 MsG0380015715.01.T01:CDS
CACAATCATGACATGCATAT+GGG 0.493273 3:+74160637 None:intergenic
GGACTAGCTGCGTGCAGATA+CGG 0.497658 3:-74158656 MsG0380015715.01.T01:exon
ATGAACCTTGAGTCGTCAAC+CGG 0.505318 3:+74160774 None:intergenic
AATCCAAATCCCACCGCGAT+AGG 0.505580 3:-74160719 MsG0380015715.01.T01:intron
TTGGACCGGTTGACGACTCA+AGG 0.526396 3:-74160779 MsG0380015715.01.T01:intron
GCCTATGTCTGATGAAGAAG+AGG 0.532134 3:-74160816 MsG0380015715.01.T01:intron
CATGTGAAATGCATGGAAAA+GGG 0.549839 3:-74159801 MsG0380015715.01.T01:CDS
GATAGGAAAAGAGAAAGGTT+TGG 0.551662 3:-74160702 MsG0380015715.01.T01:intron
TCCTCTTCTTCATCAGACAT+AGG 0.554962 3:+74160815 None:intergenic
TTTCCCTGGTCACTATCTTG+TGG 0.577317 3:+74160740 None:intergenic
AACCTGAAACTGAATTGGAC+CGG 0.581932 3:-74160793 MsG0380015715.01.T01:intron
AATGGAACAGGAGAACAAAA+CGG 0.584567 3:-74160612 MsG0380015715.01.T01:intron
TAGTTCCAACTCTCAAAATG+AGG 0.586067 3:-74158777 MsG0380015715.01.T01:CDS
ATTGTTGCATGTGAAATGCA+TGG 0.619220 3:-74159808 MsG0380015715.01.T01:CDS
GCAATAAAGAAATGAGTATG+GGG 0.621508 3:-74160571 MsG0380015715.01.T01:intron
ACCGCGATAGGAAAAGAGAA+AGG 0.627915 3:-74160707 MsG0380015715.01.T01:intron
CCATATGCATGTCATGATTG+TGG 0.638276 3:-74160636 MsG0380015715.01.T01:intron
TCAACCACAAGATAGTGACC+AGG 0.641180 3:-74160744 MsG0380015715.01.T01:intron
GGAAAAGGGATAGAGTTACA+AGG 0.646371 3:-74159787 MsG0380015715.01.T01:intron
GGAACTAGGAGAGAGCTCCA+AGG 0.654013 3:+74158793 None:intergenic
GTTTGGAAGATGTAGAAATG+GGG 0.658874 3:-74160685 MsG0380015715.01.T01:intron
CAACCACAAGATAGTGACCA+GGG 0.674930 3:-74160743 MsG0380015715.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAATTAATTTATTAT+CGG + Chr3:74159935-74159954 None:intergenic 0.0%
!!! TTTTAATTTATTTATTTTTT+TGG + Chr3:74160218-74160237 None:intergenic 0.0%
!!! TTAAGTTTTAAGAATAAATA+GGG - Chr3:74159242-74159261 MsG0380015715.01.T01:intron 10.0%
!! AATATTATTCTGTAATCAAA+AGG - Chr3:74159354-74159373 MsG0380015715.01.T01:intron 15.0%
!! ATATTATTCTGTAATCAAAA+GGG - Chr3:74159355-74159374 MsG0380015715.01.T01:intron 15.0%
!! ATTGAAAATGATAATAACAA+TGG + Chr3:74160566-74160585 None:intergenic 15.0%
!! GTAATTGAAAAATTTAATTG+AGG - Chr3:74159534-74159553 MsG0380015715.01.T01:intron 15.0%
!! TGATATAGAAGAATAAAATA+GGG + Chr3:74159386-74159405 None:intergenic 15.0%
!!! AAAATTTGATATTCGTTTTT+AGG - Chr3:74159739-74159758 MsG0380015715.01.T01:intron 15.0%
!!! AAATTTGATATTCGTTTTTA+GGG - Chr3:74159740-74159759 MsG0380015715.01.T01:intron 15.0%
!!! ATGATTTATGAAATTTTGAA+TGG - Chr3:74160132-74160151 MsG0380015715.01.T01:intron 15.0%
!!! GTTAAGTTTTAAGAATAAAT+AGG - Chr3:74159241-74159260 MsG0380015715.01.T01:intron 15.0%
!!! TAAGTTTTAAGAATAAATAG+GGG - Chr3:74159243-74159262 MsG0380015715.01.T01:intron 15.0%
!!! TATTTTTGAGAAAAAAATAC+AGG + Chr3:74158961-74158980 None:intergenic 15.0%
!!! TGTTTTTTTAATCTATTACT+GGG + Chr3:74159500-74159519 None:intergenic 15.0%
!!! TTGTTTTTTTAATCTATTAC+TGG + Chr3:74159501-74159520 None:intergenic 15.0%
!!! TTTTTTTTTTTGAAGTTCAT+GGG + Chr3:74160851-74160870 None:intergenic 15.0%
!!! TTTTTTTTTTTTGAAGTTCA+TGG + Chr3:74160852-74160871 None:intergenic 15.0%
!! ACATTAATAAGTACTTATAC+TGG + Chr3:74160713-74160732 None:intergenic 20.0%
!! ATAAATCATACATAATCCAT+AGG + Chr3:74160121-74160140 None:intergenic 20.0%
!! ATAATTAATTAAGTCAGTAG+TGG - Chr3:74159215-74159234 MsG0380015715.01.T01:intron 20.0%
!! CTGATATAGAAGAATAAAAT+AGG + Chr3:74159387-74159406 None:intergenic 20.0%
!! TAATTAATTAAGTCAGTAGT+GGG - Chr3:74159216-74159235 MsG0380015715.01.T01:intron 20.0%
!! TTTAAGTAATAAGATGTCTA+AGG + Chr3:74159967-74159986 None:intergenic 20.0%
!!! AATTTGATATTCGTTTTTAG+GGG - Chr3:74159741-74159760 MsG0380015715.01.T01:intron 20.0%
!!! GAAAAATTTAATTGAGGTTT+TGG - Chr3:74159540-74159559 MsG0380015715.01.T01:intron 20.0%
!!! GTTTAAAAGTTGAAGTTTTT+TGG - Chr3:74160168-74160187 MsG0380015715.01.T01:intron 20.0%
!!! TTTTAACTTAGATAAGCTAA+AGG + Chr3:74160415-74160434 None:intergenic 20.0%
!!! TTTTATTCTTCTATATCAGT+TGG - Chr3:74159388-74159407 MsG0380015715.01.T01:intron 20.0%
! ACATCATAAAACATATCCTA+TGG - Chr3:74160102-74160121 MsG0380015715.01.T01:intron 25.0%
! AGTAGTTAATTATGCAGTTA+TGG - Chr3:74159171-74159190 MsG0380015715.01.T01:intron 25.0%
! TACCCATTATTATATGGATA+CGG - Chr3:74159869-74159888 MsG0380015715.01.T01:CDS 25.0%
! TGCAATAAAGAAATGAGTAT+GGG - Chr3:74158890-74158909 MsG0380015715.01.T01:intron 25.0%
! TTGCAATAAAGAAATGAGTA+TGG - Chr3:74158889-74158908 MsG0380015715.01.T01:intron 25.0%
! TTTGACTACCCATTATTATA+TGG - Chr3:74159863-74159882 MsG0380015715.01.T01:CDS 25.0%
!! AATTTTGAATGGATGATGTA+TGG - Chr3:74160143-74160162 MsG0380015715.01.T01:intron 25.0%
!! GTCATTATACTTCAAGTTAT+GGG - Chr3:74159039-74159058 MsG0380015715.01.T01:intron 25.0%
!! TGTCATTATACTTCAAGTTA+TGG - Chr3:74159038-74159057 MsG0380015715.01.T01:intron 25.0%
!!! ATTTATTTGGCGTTTTAAAC+CGG - Chr3:74158607-74158626 MsG0380015715.01.T01:exon 25.0%
!!! TTTTTGAGTGTTTTCTTTGA+AGG - Chr3:74159571-74159590 MsG0380015715.01.T01:intron 25.0%
AATAATATTGCAGAACTTCG+AGG + Chr3:74159343-74159362 None:intergenic 30.0%
ACAAACAGAGTATATGATCA+AGG - Chr3:74158986-74159005 MsG0380015715.01.T01:intron 30.0%
ATAATATTGCAGAACTTCGA+GGG + Chr3:74159342-74159361 None:intergenic 30.0%
ATCAATGAAGAATCTTAGGT+GGG + Chr3:74160019-74160038 None:intergenic 30.0%
GAACCATATACATAAAAGGA+GGG + Chr3:74160390-74160409 None:intergenic 30.0%
GAGTTGCAAATATAAGGAAT+GGG - Chr3:74159440-74159459 MsG0380015715.01.T01:intron 30.0%
GCAATAAAGAAATGAGTATG+GGG - Chr3:74158891-74158910 MsG0380015715.01.T01:intron 30.0%
GGTTATCAATGAAGAATCTT+AGG + Chr3:74160023-74160042 None:intergenic 30.0%
GTCCGTATCCATATAATAAT+GGG + Chr3:74159874-74159893 None:intergenic 30.0%
GTTGTATCTAACCTGTTTAA+TGG - Chr3:74160478-74160497 MsG0380015715.01.T01:intron 30.0%
TAACTTAGATAAGCTAAAGG+AGG + Chr3:74160412-74160431 None:intergenic 30.0%
TATCAATGAAGAATCTTAGG+TGG + Chr3:74160020-74160039 None:intergenic 30.0%
TATCCACGGGTATAATAAAT+GGG - Chr3:74159838-74159857 MsG0380015715.01.T01:CDS 30.0%
TGAGTTGCAAATATAAGGAA+TGG - Chr3:74159439-74159458 MsG0380015715.01.T01:intron 30.0%
TGTCCGTATCCATATAATAA+TGG + Chr3:74159875-74159894 None:intergenic 30.0%
TTATTATATGGATACGGACA+CGG - Chr3:74159875-74159894 MsG0380015715.01.T01:intron 30.0%
! CGGTAAAATTTGCTTCAATA+AGG + Chr3:74159996-74160015 None:intergenic 30.0%
! GTTTTATGATGTGAATGAAG+TGG + Chr3:74160094-74160113 None:intergenic 30.0%
AAAAAATTCATGGGTACCCT+GGG - Chr3:74160234-74160253 MsG0380015715.01.T01:intron 35.0%
AAGAATAAATAGGGGAGAGA+AGG - Chr3:74159251-74159270 MsG0380015715.01.T01:intron 35.0%
AATAAATGCAAGCGAGAACA+TGG + Chr3:74158594-74158613 None:intergenic 35.0%
AATGGAACAGGAGAACAAAA+CGG - Chr3:74158850-74158869 MsG0380015715.01.T01:intron 35.0%
AGAATAAATAGGGGAGAGAA+GGG - Chr3:74159252-74159271 MsG0380015715.01.T01:intron 35.0%
AGGTTTGGAAGATGTAGAAA+TGG - Chr3:74158775-74158794 MsG0380015715.01.T01:CDS 35.0%
ATAAAAGGAGGGATGTCAAT+GGG + Chr3:74160379-74160398 None:intergenic 35.0%
ATACCCATTTATTATACCCG+TGG + Chr3:74159844-74159863 None:intergenic 35.0%
ATTGTTGCATGTGAAATGCA+TGG - Chr3:74159654-74159673 MsG0380015715.01.T01:intron 35.0%
CACAATCATGACATGCATAT+GGG + Chr3:74158828-74158847 None:intergenic 35.0%
CATGTGAAATGCATGGAAAA+GGG - Chr3:74159661-74159680 MsG0380015715.01.T01:intron 35.0%
CGGGTATCATATTTATCCAA+CGG - Chr3:74160315-74160334 MsG0380015715.01.T01:intron 35.0%
GATAGGAAAAGAGAAAGGTT+TGG - Chr3:74158760-74158779 MsG0380015715.01.T01:exon 35.0%
GGAACCATATACATAAAAGG+AGG + Chr3:74160391-74160410 None:intergenic 35.0%
GGAGGAACCATATACATAAA+AGG + Chr3:74160394-74160413 None:intergenic 35.0%
GGGTATCATATTTATCCAAC+GGG - Chr3:74160316-74160335 MsG0380015715.01.T01:intron 35.0%
GGTATCATATTTATCCAACG+GGG - Chr3:74160317-74160336 MsG0380015715.01.T01:intron 35.0%
GGTTTGGAAGATGTAGAAAT+GGG - Chr3:74158776-74158795 MsG0380015715.01.T01:CDS 35.0%
GTATCCACGGGTATAATAAA+TGG - Chr3:74159837-74159856 MsG0380015715.01.T01:CDS 35.0%
GTTTGGAAGATGTAGAAATG+GGG - Chr3:74158777-74158796 MsG0380015715.01.T01:CDS 35.0%
TAAAAAATTCATGGGTACCC+TGG - Chr3:74160233-74160252 MsG0380015715.01.T01:intron 35.0%
TAATGGCTACCCCAAAAATT+AGG - Chr3:74160495-74160514 MsG0380015715.01.T01:intron 35.0%
TAGTTCCAACTCTCAAAATG+AGG - Chr3:74160685-74160704 MsG0380015715.01.T01:intron 35.0%
TGATGTGAATGAAGTGGAAA+CGG + Chr3:74160088-74160107 None:intergenic 35.0%
TGTTCTCGCTTGCATTTATT+TGG - Chr3:74158594-74158613 MsG0380015715.01.T01:exon 35.0%
TTTGATTGTACTCATACCCA+CGG - Chr3:74159899-74159918 MsG0380015715.01.T01:intron 35.0%
! ACAGCAAAGCCTAATTTTTG+GGG + Chr3:74160507-74160526 None:intergenic 35.0%
! CATCCCTCCTTTTATGTATA+TGG - Chr3:74160384-74160403 MsG0380015715.01.T01:intron 35.0%
! GACAGCAAAGCCTAATTTTT+GGG + Chr3:74160508-74160527 None:intergenic 35.0%
! TGACAGCAAAGCCTAATTTT+TGG + Chr3:74160509-74160528 None:intergenic 35.0%
! TTAATTAAGTCAGTAGTGGG+AGG - Chr3:74159219-74159238 MsG0380015715.01.T01:intron 35.0%
! TTCTTTTCGTGTGATGATAC+TGG - Chr3:74159703-74159722 MsG0380015715.01.T01:intron 35.0%
!! TCATTTTGAGAGTTGGAACT+AGG + Chr3:74160686-74160705 None:intergenic 35.0%
!! TTGATATTCGTTTTTAGGGG+TGG - Chr3:74159744-74159763 MsG0380015715.01.T01:intron 35.0%
!! TTGTGGTAATTGGTGTTGTT+GGG - Chr3:74160456-74160475 MsG0380015715.01.T01:intron 35.0%
!! TTTGTGGTAATTGGTGTTGT+TGG - Chr3:74160455-74160474 MsG0380015715.01.T01:intron 35.0%
!!! GCTGTCATTTTCATATGCAT+GGG - Chr3:74160522-74160541 MsG0380015715.01.T01:intron 35.0%
!!! TGCTGTCATTTTCATATGCA+TGG - Chr3:74160521-74160540 MsG0380015715.01.T01:intron 35.0%
AAAGATTCACCTTGTCCTCA+AGG + Chr3:74159094-74159113 None:intergenic 40.0%
AACCTGAAACTGAATTGGAC+CGG - Chr3:74158669-74158688 MsG0380015715.01.T01:exon 40.0%
AATGAAGAATCTTAGGTGGG+CGG + Chr3:74160016-74160035 None:intergenic 40.0%
AGAGATAAGGGGAACTACTA+TGG - Chr3:74158916-74158935 MsG0380015715.01.T01:intron 40.0%
AGAGGAACCTGAAACTGAAT+TGG - Chr3:74158664-74158683 MsG0380015715.01.T01:exon 40.0%
AGGACAAGGTGAATCTTTAG+GGG - Chr3:74159096-74159115 MsG0380015715.01.T01:intron 40.0%
ATACAGGATGATTGATGTCG+TGG + Chr3:74158945-74158964 None:intergenic 40.0%
ATCACTTCCATAGAAACCTG+AGG + Chr3:74160636-74160655 None:intergenic 40.0%
ATCATATTTATCCAACGGGG+CGG - Chr3:74160320-74160339 MsG0380015715.01.T01:intron 40.0%
ATTACCACAAAGTTGCACTC+TGG + Chr3:74160446-74160465 None:intergenic 40.0%
CATAAAAGGAGGGATGTCAA+TGG + Chr3:74160380-74160399 None:intergenic 40.0%
CATGATTGTGGAAATGGAAC+AGG - Chr3:74158838-74158857 MsG0380015715.01.T01:intron 40.0%
CCACAATCATGACATGCATA+TGG + Chr3:74158829-74158848 None:intergenic 40.0%
CCATATGCATGTCATGATTG+TGG - Chr3:74158826-74158845 MsG0380015715.01.T01:CDS 40.0%
CCTGCTGAGTTGCAAATATA+AGG - Chr3:74159434-74159453 MsG0380015715.01.T01:intron 40.0%
CCTTATATTTGCAACTCAGC+AGG + Chr3:74159437-74159456 None:intergenic 40.0%
CTTACTGTGTTTCTCTTGCA+TGG - Chr3:74160785-74160804 MsG0380015715.01.T01:intron 40.0%
GAGATAAGGGGAACTACTAT+GGG - Chr3:74158917-74158936 MsG0380015715.01.T01:intron 40.0%
GAGGACAAGGTGAATCTTTA+GGG - Chr3:74159095-74159114 MsG0380015715.01.T01:intron 40.0%
GAGTGCAACTTTGTGGTAAT+TGG - Chr3:74160446-74160465 MsG0380015715.01.T01:intron 40.0%
GATATCATAAGTGTCCACCT+GGG + Chr3:74159066-74159085 None:intergenic 40.0%
GCATGTCATGATTGTGGAAA+TGG - Chr3:74158832-74158851 MsG0380015715.01.T01:intron 40.0%
GCATGTGAAATGCATGGAAA+AGG - Chr3:74159660-74159679 MsG0380015715.01.T01:intron 40.0%
GGAAAAGGGATAGAGTTACA+AGG - Chr3:74159675-74159694 MsG0380015715.01.T01:intron 40.0%
TCCTCTTCTTCATCAGACAT+AGG + Chr3:74158650-74158669 None:intergenic 40.0%
TGAGGACAAGGTGAATCTTT+AGG - Chr3:74159094-74159113 MsG0380015715.01.T01:intron 40.0%
TGATCATTGTCCTGTTACCT+TGG - Chr3:74160652-74160671 MsG0380015715.01.T01:intron 40.0%
TTATGATATCCTCCACCTTG+AGG - Chr3:74159076-74159095 MsG0380015715.01.T01:intron 40.0%
TTGATTGTACTCATACCCAC+GGG - Chr3:74159900-74159919 MsG0380015715.01.T01:intron 40.0%
! ACCTTTCTCTTTTCCTATCG+CGG + Chr3:74158759-74158778 None:intergenic 40.0%
!! GCGTTTTAAACCGGAATATC+GGG - Chr3:74158616-74158635 MsG0380015715.01.T01:exon 40.0%
!! GGCGTTTTAAACCGGAATAT+CGG - Chr3:74158615-74158634 MsG0380015715.01.T01:exon 40.0%
!! TGGATCCTCATTTTGAGAGT+TGG + Chr3:74160693-74160712 None:intergenic 40.0%
!!! TGTTTTCTTTGAAGGTACCG+CGG - Chr3:74159579-74159598 MsG0380015715.01.T01:intron 40.0%
!!! TTTTGGGGTAGCCATTAAAC+AGG + Chr3:74160492-74160511 None:intergenic 40.0%
AACCGGTCCAATTCAGTTTC+AGG + Chr3:74158674-74158693 None:intergenic 45.0%
AACCTGAGGAGACAACAACA+AGG + Chr3:74160622-74160641 None:intergenic 45.0%
ACCGCGATAGGAAAAGAGAA+AGG - Chr3:74158755-74158774 MsG0380015715.01.T01:exon 45.0%
AGTATGGGGTCAGAGATAAG+GGG - Chr3:74158905-74158924 MsG0380015715.01.T01:intron 45.0%
ATCATGCTATCCGTACCCAT+GGG - Chr3:74160351-74160370 MsG0380015715.01.T01:intron 45.0%
ATCTGAGGATTTGACTGGGA+AGG - Chr3:74159288-74159307 MsG0380015715.01.T01:intron 45.0%
ATGAACCTTGAGTCGTCAAC+CGG + Chr3:74158691-74158710 None:intergenic 45.0%
CAACCACAAGATAGTGACCA+GGG - Chr3:74158719-74158738 MsG0380015715.01.T01:exon 45.0%
GAATCCAGAGTGCAACTTTG+TGG - Chr3:74160439-74160458 MsG0380015715.01.T01:intron 45.0%
GAGTATGGGGTCAGAGATAA+GGG - Chr3:74158904-74158923 MsG0380015715.01.T01:intron 45.0%
GCCTATGTCTGATGAAGAAG+AGG - Chr3:74158646-74158665 MsG0380015715.01.T01:exon 45.0%
GGACAAGGTGAATCTTTAGG+GGG - Chr3:74159097-74159116 MsG0380015715.01.T01:intron 45.0%
GGATATCATAAGTGTCCACC+TGG + Chr3:74159067-74159086 None:intergenic 45.0%
GGCATGATATCCCGATATTC+CGG + Chr3:74158629-74158648 None:intergenic 45.0%
GTTGTCTCCTCAGGTTTCTA+TGG - Chr3:74160626-74160645 MsG0380015715.01.T01:intron 45.0%
TATCATGCTATCCGTACCCA+TGG - Chr3:74160350-74160369 MsG0380015715.01.T01:intron 45.0%
TCAACCACAAGATAGTGACC+AGG - Chr3:74158718-74158737 MsG0380015715.01.T01:exon 45.0%
TCATATTTATCCAACGGGGC+GGG - Chr3:74160321-74160340 MsG0380015715.01.T01:intron 45.0%
TCCGTGGGTAAAATCCATCA+CGG - Chr3:74159791-74159810 MsG0380015715.01.T01:CDS 45.0%
TCTGAGGATTTGACTGGGAA+GGG - Chr3:74159289-74159308 MsG0380015715.01.T01:intron 45.0%
TGAGTATGGGGTCAGAGATA+AGG - Chr3:74158903-74158922 MsG0380015715.01.T01:intron 45.0%
TTTCCCTGGTCACTATCTTG+TGG + Chr3:74158725-74158744 None:intergenic 45.0%
! ACAAGGTGAATCTTTAGGGG+GGG - Chr3:74159099-74159118 MsG0380015715.01.T01:intron 45.0%
! CTATTCTCATCCGTTTTCCG+CGG + Chr3:74159599-74159618 None:intergenic 45.0%
! GACAAGGTGAATCTTTAGGG+GGG - Chr3:74159098-74159117 MsG0380015715.01.T01:intron 45.0%
! TACTTCAAGTTATGGGACCC+AGG - Chr3:74159046-74159065 MsG0380015715.01.T01:intron 45.0%
! TTCTCTTTTCCTATCGCGGT+GGG + Chr3:74158755-74158774 None:intergenic 45.0%
! TTTCTCTTTTCCTATCGCGG+TGG + Chr3:74158756-74158775 None:intergenic 45.0%
!! AAGGGGCCATTTTGGCATTA+GGG - Chr3:74159307-74159326 MsG0380015715.01.T01:intron 45.0%
!! TTTGAAGGTACCGCGGAAAA+CGG - Chr3:74159586-74159605 MsG0380015715.01.T01:intron 45.0%
!! AAAAAATTCTAAATAAAAAT+AGG + Chr3:74160044-74160063 None:intergenic 5.0%
!! AAATAAATTAAAAAATTCAT+GGG - Chr3:74160225-74160244 MsG0380015715.01.T01:intron 5.0%
!! AATTTAATTAATTTATTATC+GGG + Chr3:74159934-74159953 None:intergenic 5.0%
!! TAAATAAATTAAAAAATTCA+TGG - Chr3:74160224-74160243 MsG0380015715.01.T01:intron 5.0%
AATCCAAATCCCACCGCGAT+AGG - Chr3:74158743-74158762 MsG0380015715.01.T01:exon 50.0%
CATATTTATCCAACGGGGCG+GGG - Chr3:74160322-74160341 MsG0380015715.01.T01:intron 50.0%
CCCTCGTGGTATCCACTATA+CGG + Chr3:74160282-74160301 None:intergenic 50.0%
CCGTGGGTAAAATCCATCAC+GGG - Chr3:74159792-74159811 MsG0380015715.01.T01:CDS 50.0%
CCTCGTGGTATCCACTATAC+GGG + Chr3:74160281-74160300 None:intergenic 50.0%
CTGAGGATTTGACTGGGAAG+GGG - Chr3:74159290-74159309 MsG0380015715.01.T01:intron 50.0%
GATTCACCTTGTCCTCAAGG+TGG + Chr3:74159091-74159110 None:intergenic 50.0%
GCAAAAATACCCATGCCCAC+GGG - Chr3:74159766-74159785 MsG0380015715.01.T01:intron 50.0%
GCACCTCCCTAATGCCAAAA+TGG + Chr3:74159316-74159335 None:intergenic 50.0%
GGAGAGAAGGGAAGATTGAG+GGG - Chr3:74159264-74159283 MsG0380015715.01.T01:intron 50.0%
GGATGTCAATGGGTACCCAT+GGG + Chr3:74160369-74160388 None:intergenic 50.0%
GGCAAAAATACCCATGCCCA+CGG - Chr3:74159765-74159784 MsG0380015715.01.T01:intron 50.0%
GGGAGAGAAGGGAAGATTGA+GGG - Chr3:74159263-74159282 MsG0380015715.01.T01:intron 50.0%
GGGCATCTGAGGATTTGACT+GGG - Chr3:74159284-74159303 MsG0380015715.01.T01:intron 50.0%
GGTAAAATCCATCACGGGCA+CGG - Chr3:74159797-74159816 MsG0380015715.01.T01:CDS 50.0%
GGTAGCTAAACGGGTATCCA+CGG - Chr3:74159824-74159843 MsG0380015715.01.T01:CDS 50.0%
GTAAAATCCATCACGGGCAC+GGG - Chr3:74159798-74159817 MsG0380015715.01.T01:CDS 50.0%
GTAGCTAAACGGGTATCCAC+GGG - Chr3:74159825-74159844 MsG0380015715.01.T01:CDS 50.0%
TAAAATCCATCACGGGCACG+GGG - Chr3:74159799-74159818 MsG0380015715.01.T01:CDS 50.0%
TATCCTCCACCTTGAGGACA+AGG - Chr3:74159082-74159101 MsG0380015715.01.T01:intron 50.0%
TATCGGGTATGAGTACCCGT+GGG + Chr3:74159918-74159937 None:intergenic 50.0%
TCAATGGGTACCCATGGGTA+CGG + Chr3:74160364-74160383 None:intergenic 50.0%
TTATCGGGTATGAGTACCCG+TGG + Chr3:74159919-74159938 None:intergenic 50.0%
TTTCCTATCGCGGTGGGATT+TGG + Chr3:74158749-74158768 None:intergenic 50.0%
! ATTTTACCCACGGATACCCG+TGG + Chr3:74159785-74159804 None:intergenic 50.0%
! TAGTGGATACCACGAGGGTA+CGG - Chr3:74160284-74160303 MsG0380015715.01.T01:intron 50.0%
! TTCAAGTTATGGGACCCAGG+TGG - Chr3:74159049-74159068 MsG0380015715.01.T01:intron 50.0%
! TTTTACCCACGGATACCCGT+GGG + Chr3:74159784-74159803 None:intergenic 50.0%
!! CCGTATAGTGGATACCACGA+GGG - Chr3:74160279-74160298 MsG0380015715.01.T01:intron 50.0%
!! GAAGGGGCCATTTTGGCATT+AGG - Chr3:74159306-74159325 MsG0380015715.01.T01:intron 50.0%
!! GACCTTGTTGTTGTCTCCTC+AGG - Chr3:74160617-74160636 MsG0380015715.01.T01:intron 50.0%
!!! CCCGTGATGGATTTTACCCA+CGG + Chr3:74159795-74159814 None:intergenic 50.0%
ATCCACTATACGGGTACCCG+CGG + Chr3:74160272-74160291 None:intergenic 55.0%
CGGTGGGATTTGGATTTCCC+TGG + Chr3:74158739-74158758 None:intergenic 55.0%
CTGGGATACCTCAATACCCG+CGG - Chr3:74160252-74160271 MsG0380015715.01.T01:intron 55.0%
GGAACTAGGAGAGAGCTCCA+AGG + Chr3:74160672-74160691 None:intergenic 55.0%
GGAGAGAGCTCCAAGGTAAC+AGG + Chr3:74160665-74160684 None:intergenic 55.0%
GGGATGTCAATGGGTACCCA+TGG + Chr3:74160370-74160389 None:intergenic 55.0%
GGGGAGAGAAGGGAAGATTG+AGG - Chr3:74159262-74159281 MsG0380015715.01.T01:intron 55.0%
TCACCTTGTCCTCAAGGTGG+AGG + Chr3:74159088-74159107 None:intergenic 55.0%
TGGGATACCTCAATACCCGC+GGG - Chr3:74160253-74160272 MsG0380015715.01.T01:intron 55.0%
TTGGACCGGTTGACGACTCA+AGG - Chr3:74158683-74158702 MsG0380015715.01.T01:exon 55.0%
! AGTGGATACCACGAGGGTAC+GGG - Chr3:74160285-74160304 MsG0380015715.01.T01:intron 55.0%
! GGGGCATCTGAGGATTTGAC+TGG - Chr3:74159283-74159302 MsG0380015715.01.T01:intron 55.0%
! TGACTGGGAAGGGGCCATTT+TGG - Chr3:74159299-74159318 MsG0380015715.01.T01:intron 55.0%
!! CCCGTATAGTGGATACCACG+AGG - Chr3:74160278-74160297 MsG0380015715.01.T01:intron 55.0%
!! GCGGGTATTGAGGTATCCCA+GGG + Chr3:74160253-74160272 None:intergenic 55.0%
!! GGAAGATTGAGGGGCATCTG+AGG - Chr3:74159273-74159292 MsG0380015715.01.T01:intron 55.0%
!! GGACTAGCTGCGTGCAGATA+CGG - Chr3:74160806-74160825 MsG0380015715.01.T01:intron 55.0%
!! GGGCCATTTTGGCATTAGGG+AGG - Chr3:74159310-74159329 MsG0380015715.01.T01:intron 55.0%
TCCACTATACGGGTACCCGC+GGG + Chr3:74160271-74160290 None:intergenic 60.0%
! GTGGATACCACGAGGGTACG+GGG - Chr3:74160286-74160305 MsG0380015715.01.T01:intron 60.0%
!! CGCGGGTATTGAGGTATCCC+AGG + Chr3:74160254-74160273 None:intergenic 60.0%
AATCCATCACGGGCACGGGG+CGG - Chr3:74159802-74159821 MsG0380015715.01.T01:CDS 65.0%
ACCCGCGGGTACCCGTATAG+TGG - Chr3:74160267-74160286 MsG0380015715.01.T01:intron 65.0%
CACGGGGCGGGTAGCTAAAC+GGG - Chr3:74159815-74159834 MsG0380015715.01.T01:CDS 65.0%
CCACGGATACCCGTGGGCAT+GGG + Chr3:74159778-74159797 None:intergenic 65.0%
CCATGCCCACGGGTATCCGT+GGG - Chr3:74159776-74159795 MsG0380015715.01.T01:intron 65.0%
GCACGGGGCGGGTAGCTAAA+CGG - Chr3:74159814-74159833 MsG0380015715.01.T01:CDS 65.0%
TTATCCAACGGGGCGGGGAC+TGG - Chr3:74160327-74160346 MsG0380015715.01.T01:intron 65.0%
! ACGGGTACCCGCGGGTATTG+AGG + Chr3:74160263-74160282 None:intergenic 65.0%
! GATACCACGAGGGTACGGGG+CGG - Chr3:74160289-74160308 MsG0380015715.01.T01:intron 65.0%
ATCCATCACGGGCACGGGGC+GGG - Chr3:74159803-74159822 MsG0380015715.01.T01:CDS 70.0%
CCCACGGATACCCGTGGGCA+TGG + Chr3:74159779-74159798 None:intergenic 70.0%
CCCATGCCCACGGGTATCCG+TGG - Chr3:74159775-74159794 MsG0380015715.01.T01:intron 70.0%
GATACCAGTCCCCGCCCCGT+TGG + Chr3:74160334-74160353 None:intergenic 70.0%
! ACGAGGGTACGGGGCGGACA+CGG - Chr3:74160295-74160314 MsG0380015715.01.T01:intron 70.0%
GTGTCCGCCCCGTACCCTCG+TGG + Chr3:74160296-74160315 None:intergenic 75.0%
TACCCGCCCCGTGCCCGTGA+TGG + Chr3:74159808-74159827 None:intergenic 75.0%
! CGAGGGTACGGGGCGGACAC+GGG - Chr3:74160296-74160315 MsG0380015715.01.T01:intron 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 74158593 74160891 74158593 ID=MsG0380015716.01;Name=MsG0380015716.01
Chr3 mRNA 74158593 74160891 74158593 ID=MsG0380015716.01.T01;Parent=MsG0380015716.01;Name=MsG0380015716.01.T01;_AED=0.49;_eAED=0.68;_QI=0|0.5|0.66|1|1|1|3|173|167
Chr3 exon 74158593 74158845 74158593 ID=MsG0380015716.01.T01:exon:897;Parent=MsG0380015716.01.T01
Chr3 exon 74159788 74159891 74159788 ID=MsG0380015716.01.T01:exon:896;Parent=MsG0380015716.01.T01
Chr3 exon 74160572 74160891 74160572 ID=MsG0380015716.01.T01:exon:895;Parent=MsG0380015716.01.T01
Chr3 CDS 74160572 74160891 74160572 ID=MsG0380015716.01.T01:cds;Parent=MsG0380015716.01.T01
Chr3 CDS 74159788 74159891 74159788 ID=MsG0380015716.01.T01:cds;Parent=MsG0380015716.01.T01
Chr3 CDS 74158766 74158845 74158766 ID=MsG0380015716.01.T01:cds;Parent=MsG0380015716.01.T01
Chr3 three_prime_UTR 74158593 74158765 74158593 ID=MsG0380015716.01.T01:three_prime_utr;Parent=MsG0380015716.01.T01
Gene Sequence

>MsG0380015716.01.T01

ATGTTCTCGCTTGCATTTATTTGGCGTTTTAAACCGGAATATCGGGATATCATGCCTATGTCTGATGAAGAAGAGGAACCTGAAACTGAATTGGACCGGTTGACGACTCAAGGTTCATTCAATATTCAACCACAAGATAGTGACCAGGGAAATCCAAATCCCACCGCGATAGGAAAAGAGAAAGGTTTGGAAGATGTAGAAATGGGGAAAATAGAGAGATATGTAGAAAATGCCCATATGCATGTCATGATTGTGGAAATGGAACAGGAGAACAAAACGGATTTGTGCAAACTTATTTGCAATAAAGAAATGAGTATGGGGTACCGCGGAAAACGGATGAGAATAGACTATTTCATAGTTTCAGAAAAACTTAAAGAAAAGATTGTTGCATGTGAAATGCATGGAAAAGGGATAGAGTTACAAGGTTTCTATGGAAGTGATCATTGTCCTGTTACCTTGGAGCTCTCTCCTAGTTCCAACTCTCAAAATGAGGATCCAGTATAA
Protein sequence

>MsG0380015716.01.T01

MFSLAFIWRFKPEYRDIMPMSDEEEEPETELDRLTTQGSFNIQPQDSDQGNPNPTAIGKEKGLEDVEMGKIERYVENAHMHVMIVEMEQENKTDLCKLICNKEMSMGYRGKRMRIDYFIVSEKLKEKIVACEMHGKGIELQGFYGSDHCPVTLELSPSSNSQNEDPV*