AlfalfaGEDB | MsG0880042310.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042310.01

KEGG: -
GO: -
PFAM: PF09409

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042310.01.T01	XP_013444168.1	95.238	126	6	0	30	155	182	307	3.21E-81	252

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042310.01.T01	A0A072TXC9	95.238	126	6	0	30	155	182	307	1.54e-81	252

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048462.01	MsG0880042310.01	0.809725	1.641679e-50	9.712956e-48
MsG0080048856.01	MsG0880042310.01	0.812541	4.024624e-51	2.566073e-48
MsG0180004099.01	MsG0880042310.01	0.825929	3.584650e-54	3.313568e-51
MsG0180004389.01	MsG0880042310.01	0.811170	8.002210e-51	4.918452e-48
MsG0180004754.01	MsG0880042310.01	0.807241	5.564880e-50	3.083749e-47
MsG0180005250.01	MsG0880042310.01	0.812590	3.927988e-51	2.507514e-48
MsG0180005445.01	MsG0880042310.01	0.807164	5.778911e-50	3.195864e-47
MsG0180005575.01	MsG0880042310.01	0.806312	8.742806e-50	4.730157e-47
MsG0780040669.01	MsG0880042310.01	0.811104	8.270981e-51	5.074953e-48
MsG0780040841.01	MsG0880042310.01	0.817567	3.083890e-52	2.253778e-49
MsG0780041535.01	MsG0880042310.01	0.802372	5.788508e-49	2.830880e-46
MsG0880042310.01	MsG0880042311.01	0.842084	3.209482e-58	4.812379e-55
MsG0880042310.01	MsG0880043480.01	0.819055	1.419983e-52	1.081191e-49
MsG0880042310.01	MsG0880046012.01	0.810111	1.355964e-50	8.104088e-48
MsG0880042310.01	MsG0880047472.01	0.804510	2.086441e-49	1.077730e-46
MsG0880042310.01	MsG0880047473.01	0.822292	2.561598e-53	2.135193e-50
MsG0280010698.01	MsG0880042310.01	0.805687	1.183570e-49	6.302216e-47
MsG0280010980.01	MsG0880042310.01	0.809034	2.309749e-50	1.341541e-47
MsG0280011003.01	MsG0880042310.01	0.814288	1.662758e-51	1.111188e-48
MsG0280011013.01	MsG0880042310.01	0.819888	9.166523e-53	7.143116e-50
MsG0280011014.01	MsG0880042310.01	0.815667	8.222389e-52	5.704658e-49
MsG0280011101.01	MsG0880042310.01	0.804525	2.071543e-49	1.070450e-46
MsG0380011648.01	MsG0880042310.01	0.803023	4.247945e-49	2.111986e-46
MsG0380012471.01	MsG0880042310.01	0.800934	1.141777e-48	5.383922e-46
MsG0380013475.01	MsG0880042310.01	0.840624	7.775289e-58	1.113909e-54
MsG0380013834.01	MsG0880042310.01	0.802178	6.345428e-49	3.087703e-46
MsG0380014537.01	MsG0880042310.01	0.810602	1.062366e-50	6.431915e-48
MsG0680034890.01	MsG0880042310.01	0.838497	2.776127e-57	3.725010e-54
MsG0480021465.01	MsG0880042310.01	0.807350	5.276340e-50	2.932133e-47
MsG0480022985.01	MsG0880042310.01	0.802001	6.899689e-49	3.342337e-46
MsG0480023600.01	MsG0880042310.01	0.804942	1.695698e-49	8.858072e-47
MsG0480023873.01	MsG0880042310.01	0.807383	5.192943e-50	2.888272e-47
MsG0580024506.01	MsG0880042310.01	0.802625	5.134294e-49	2.527011e-46
MsG0380015677.01	MsG0880042310.01	0.802719	4.910199e-49	2.422573e-46
MsG0480018163.01	MsG0880042310.01	0.800366	1.490451e-48	6.927549e-46
MsG0480018313.01	MsG0880042310.01	0.810154	1.327423e-50	7.942002e-48
MsG0580026309.01	MsG0880042310.01	0.805429	1.340489e-49	7.090324e-47
MsG0580027431.01	MsG0880042310.01	0.807409	5.127664e-50	2.853930e-47
MsG0580028436.01	MsG0880042310.01	0.827123	1.861570e-54	1.780634e-51
MsG0280007955.01	MsG0880042310.01	0.817153	3.822827e-52	2.762353e-49
MsG0280010489.01	MsG0880042310.01	0.802020	6.838228e-49	3.314204e-46
MsG0280010491.01	MsG0880042310.01	0.825083	5.687965e-54	5.132581e-51
MsG0780038424.01	MsG0880042310.01	0.824797	6.642071e-54	5.945090e-51
MsG0780039170.01	MsG0880042310.01	0.803523	3.347805e-49	1.686107e-46
MsG0780039243.01	MsG0880042310.01	0.816497	5.364932e-52	3.807398e-49
MsG0780039443.01	MsG0880042310.01	0.811469	6.894417e-51	4.271212e-48
MsG0780040044.01	MsG0880042310.01	0.803457	3.453746e-49	1.736457e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042310.01.T01	MTR_8g013030	95.238	126	6	0	30	155	182	307	3.89e-85	252
MsG0880042310.01.T01	MTR_8g085380	61.983	121	44	1	34	152	288	408	4.48e-51	168

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042310.01.T01	AT1G04850	60.331	121	46	1	34	152	204	324	3.50e-51	166
MsG0880042310.01.T01	AT1G04850	60.331	121	46	1	34	152	293	413	3.03e-50	166
MsG0880042310.01.T01	AT5G48690	57.143	119	48	2	39	154	150	268	6.09e-40	135
MsG0880042310.01.T01	AT5G48690	57.143	119	48	2	39	154	200	318	2.78e-39	135

Find 19 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATAGAGTTGGAAGTTTAGA+TGG	0.214147	8:-6955181	MsG0880042310.01.T01:CDS
AAATGTCTTACTTGGAATAA+TGG	0.248440	8:+6955397	None:intergenic
CTAAATCTTATACATTTCAA+TGG	0.390801	8:-6956078	MsG0880042310.01.T01:CDS
GCAATGACAAATCCTTACTT+TGG	0.409516	8:-6955043	MsG0880042310.01.T01:CDS
AGCACTTGAGAGAATGTTTG+AGG	0.424843	8:-6956012	MsG0880042310.01.T01:CDS
CTTGTATCTTCCTAGTGAAA+AGG	0.431080	8:-6955110	MsG0880042310.01.T01:CDS
GAAACACTTCTAGTGTATGT+TGG	0.482560	8:-6955475	MsG0880042310.01.T01:CDS
TTAGATAACACAATCTCAAA+AGG	0.498401	8:+6956097	None:intergenic
TTTCTTTAACAGGATAGAGT+TGG	0.506570	8:-6955193	MsG0880042310.01.T01:intron
GTACTTAAAAGTCCAAAGTA+AGG	0.507326	8:+6955031	None:intergenic
ATGGAACTACTAAGTTCAGC+TGG	0.537946	8:-6955082	MsG0880042310.01.T01:CDS
AGAGTTGGAAGTTTAGATGG+AGG	0.539241	8:-6955178	MsG0880042310.01.T01:CDS
GTAGAGTTTCTTGAGCTATG+TGG	0.542378	8:-6955154	MsG0880042310.01.T01:CDS
GTGAAAATGCAAGAATCAGA+AGG	0.554564	8:-6955504	MsG0880042310.01.T01:CDS
GTCTCTCAAGCGTGAACACA+AGG	0.558102	8:-6955990	MsG0880042310.01.T01:intron
ATACACTAGAAGTGTTTCAA+AGG	0.560419	8:+6955480	None:intergenic
TCCTAGTGAAAAGGTTGACA+TGG	0.567134	8:-6955101	MsG0880042310.01.T01:CDS
AAATGTTGTCAAGAATCCTA+AGG	0.615510	8:-6955452	MsG0880042310.01.T01:CDS
TGAAAATGCAAGAATCAGAA+GGG	0.643372	8:-6955503	MsG0880042310.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTAAAACCAATATCTTTTT+AGG	+	Chr8:6955936-6955955	None:intergenic	15.0%
!!	CAATATTCAAATTCTCTATA+GGG	-	Chr8:6955634-6955653	MsG0880042310.01.T01:intron	20.0%
!!	CATTTGAAAAAACAAATGTA+TGG	+	Chr8:6955604-6955623	None:intergenic	20.0%
!!	CTAAATCTTATACATTTCAA+TGG	-	Chr8:6955082-6955101	MsG0880042310.01.T01:CDS	20.0%
!!	TCAATATTCAAATTCTCTAT+AGG	-	Chr8:6955633-6955652	MsG0880042310.01.T01:intron	20.0%
!!!	TTTTCCAAAACAAAACATAT+TGG	-	Chr8:6955783-6955802	MsG0880042310.01.T01:intron	20.0%
!	AAAACAAAACATATTGGATG+TGG	-	Chr8:6955789-6955808	MsG0880042310.01.T01:intron	25.0%
!	AAATGTCTTACTTGGAATAA+TGG	+	Chr8:6955766-6955785	None:intergenic	25.0%
!	CTATACTTAAACATAACTCT+TGG	-	Chr8:6955263-6955282	MsG0880042310.01.T01:intron	25.0%
!	TTAGATAACACAATCTCAAA+AGG	+	Chr8:6955066-6955085	None:intergenic	25.0%
!!	GACATGTTAGTTGTTATTAT+CGG	-	Chr8:6955505-6955524	MsG0880042310.01.T01:CDS	25.0%
!!!	ACATCCAATATGTTTTGTTT+TGG	+	Chr8:6955790-6955809	None:intergenic	25.0%
	AAATGTTGTCAAGAATCCTA+AGG	-	Chr8:6955708-6955727	MsG0880042310.01.T01:intron	30.0%
	ATCCTAAGGAAGAAAAATAC+AGG	-	Chr8:6955722-6955741	MsG0880042310.01.T01:intron	30.0%
	CCACATAACATAGTAGTAAT+AGG	-	Chr8:6955454-6955473	MsG0880042310.01.T01:CDS	30.0%
	CCTATTACTACTATGTTATG+TGG	+	Chr8:6955457-6955476	None:intergenic	30.0%
	GCATGTACCTAAAAAGATAT+TGG	-	Chr8:6955926-6955945	MsG0880042310.01.T01:intron	30.0%
	GTACTTAAAAGTCCAAAGTA+AGG	+	Chr8:6956132-6956151	None:intergenic	30.0%
	TCCAATGTTTATGTTCATGA+CGG	+	Chr8:6955348-6955367	None:intergenic	30.0%
	TCTATCCTGTTAAAGAAATG+TGG	+	Chr8:6955965-6955984	None:intergenic	30.0%
	TGAAAATGCAAGAATCAGAA+GGG	-	Chr8:6955657-6955676	MsG0880042310.01.T01:intron	30.0%
	TTCTTCCACATTTCTTTAAC+AGG	-	Chr8:6955957-6955976	MsG0880042310.01.T01:intron	30.0%
	TTTCTTTAACAGGATAGAGT+TGG	-	Chr8:6955967-6955986	MsG0880042310.01.T01:intron	30.0%
!	ACTTTTGTGGTTGTATAAAC+AGG	+	Chr8:6955126-6955145	None:intergenic	30.0%
!	GTTAGTTGTTATTATCGGTA+CGG	-	Chr8:6955510-6955529	MsG0880042310.01.T01:intron	30.0%
!	TGTTATGTGGAGAAATTTTC+AGG	+	Chr8:6955444-6955463	None:intergenic	30.0%
!	TTCCTGTATTTTTCTTCCTT+AGG	+	Chr8:6955727-6955746	None:intergenic	30.0%
!!	ATACACTAGAAGTGTTTCAA+AGG	+	Chr8:6955683-6955702	None:intergenic	30.0%
!!!	GTTTTGGAAAATGTCTTACT+TGG	+	Chr8:6955774-6955793	None:intergenic	30.0%
	ACATGTCTATCAGAAACCTA+CGG	+	Chr8:6955492-6955511	None:intergenic	35.0%
	ACCGTCATGAACATAAACAT+TGG	-	Chr8:6955344-6955363	MsG0880042310.01.T01:intron	35.0%
	CTTAAACATAACTCTTGGCA+TGG	-	Chr8:6955268-6955287	MsG0880042310.01.T01:intron	35.0%
	CTTGTATCTTCCTAGTGAAA+AGG	-	Chr8:6956050-6956069	MsG0880042310.01.T01:CDS	35.0%
	GAAACACTTCTAGTGTATGT+TGG	-	Chr8:6955685-6955704	MsG0880042310.01.T01:intron	35.0%
	GATAGAGTTGGAAGTTTAGA+TGG	-	Chr8:6955979-6955998	MsG0880042310.01.T01:intron	35.0%
	GCAATGACAAATCCTTACTT+TGG	-	Chr8:6956117-6956136	MsG0880042310.01.T01:CDS	35.0%
	GTGAAAATGCAAGAATCAGA+AGG	-	Chr8:6955656-6955675	MsG0880042310.01.T01:intron	35.0%
!	GTTTATGTTCATGACGGTTT+TGG	+	Chr8:6955342-6955361	None:intergenic	35.0%
	AGAGTTGGAAGTTTAGATGG+AGG	-	Chr8:6955982-6956001	MsG0880042310.01.T01:intron	40.0%
	AGCACTTGAGAGAATGTTTG+AGG	-	Chr8:6955148-6955167	MsG0880042310.01.T01:CDS	40.0%
	ATGGAACTACTAAGTTCAGC+TGG	-	Chr8:6956078-6956097	MsG0880042310.01.T01:CDS	40.0%
	GTAGAGTTTCTTGAGCTATG+TGG	-	Chr8:6956006-6956025	MsG0880042310.01.T01:CDS	40.0%
	TCCTAGTGAAAAGGTTGACA+TGG	-	Chr8:6956059-6956078	MsG0880042310.01.T01:CDS	40.0%
	TCTATCAGAAACCTACGGAA+AGG	+	Chr8:6955487-6955506	None:intergenic	40.0%
!	TCCATGTCAACCTTTTCACT+AGG	+	Chr8:6956063-6956082	None:intergenic	40.0%
!	TCTCAAGTGCTCAACTTTTG+TGG	+	Chr8:6955139-6955158	None:intergenic	40.0%
!!	AGGATGTGTTGACTACTCAT+CGG	+	Chr8:6955424-6955443	None:intergenic	40.0%
!!!	CTATGTGGTTTTGAGAGAAG+TGG	-	Chr8:6956021-6956040	MsG0880042310.01.T01:CDS	40.0%
	CATATTGGATGTGGAGTCGT+AGG	-	Chr8:6955798-6955817	MsG0880042310.01.T01:intron	45.0%
!	GTTCCTATGAAGCACCAACA+CGG	+	Chr8:6955402-6955421	None:intergenic	45.0%
	GTCTCTCAAGCGTGAACACA+AGG	-	Chr8:6955170-6955189	MsG0880042310.01.T01:CDS	50.0%
	TAGGATGCATGCCTTTCCGT+AGG	-	Chr8:6955473-6955492	MsG0880042310.01.T01:CDS	50.0%
!	ACACCGTGTTGGTGCTTCAT+AGG	-	Chr8:6955396-6955415	MsG0880042310.01.T01:intron	50.0%
	ACGACACGTCGACACCGTGT+TGG	-	Chr8:6955385-6955404	MsG0880042310.01.T01:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	6955024	6956158	6955024	ID=MsG0880042310.01;Name=MsG0880042310.01
Chr8	mRNA	6955024	6956158	6955024	ID=MsG0880042310.01.T01;Parent=MsG0880042310.01;Name=MsG0880042310.01.T01;_AED=0.49;_eAED=0.52;_QI=0\|0\|0\|1\|1\|1\|3\|0\|155
Chr8	exon	6955991	6956158	6955991	ID=MsG0880042310.01.T01:exon:1875;Parent=MsG0880042310.01.T01
Chr8	exon	6955408	6955527	6955408	ID=MsG0880042310.01.T01:exon:1874;Parent=MsG0880042310.01.T01
Chr8	exon	6955024	6955203	6955024	ID=MsG0880042310.01.T01:exon:1873;Parent=MsG0880042310.01.T01
Chr8	CDS	6955991	6956158	6955991	ID=MsG0880042310.01.T01:cds;Parent=MsG0880042310.01.T01
Chr8	CDS	6955408	6955527	6955408	ID=MsG0880042310.01.T01:cds;Parent=MsG0880042310.01.T01
Chr8	CDS	6955024	6955203	6955024	ID=MsG0880042310.01.T01:cds;Parent=MsG0880042310.01.T01

Gene Sequence

>MsG0880042310.01.T01

TTGGATTCTCATTCTGATATTCAATCTTGTTTTCAGTTTCCTTTTGAGATTGTGTTATCTAAATCTTATACATTTCAATGGAACTTGCAGACTTCAAAGCCTGTTTATACAACCACAAAAGTTGAGCACTTGAGAGAATGTTTGAGGTCTCTCAAGCGTGAACACAAGGGTGAAAATGCAAGAATCAGAAGGGCCTTTGAAACACTTCTAGTGTATGTTGGAAATGTTGTCAAGAATCCTAAGGAAGAAAAATACAGGAAGATTAGACTGAGCAATCCATTATTCCAAGATAGAGTTGGAAGTTTAGATGGAGGTGTAGAGTTTCTTGAGCTATGTGGTTTTGAGAGAAGTGGTGATTTCTTGTATCTTCCTAGTGAAAAGGTTGACATGGAACTACTAAGTTCAGCTGGTTTTGTCTTAAATTCTGCAATGACAAATCCTTACTTTGGACTTTTAAGTACTTGTTAA

Protein sequence

>MsG0880042310.01.T01

LDSHSDIQSCFQFPFEIVLSKSYTFQWNLQTSKPVYTTTKVEHLRECLRSLKREHKGENARIRRAFETLLVYVGNVVKNPKEEKYRKIRLSNPLFQDRVGSLDGGVEFLELCGFERSGDFLYLPSEKVDMELLSSAGFVLNSAMTNPYFGLLSTC*