AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042436.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042436.01.T01 MTR_8g018780 98.352 182 3 0 1 182 1 182 2.55e-129 360
MsG0880042436.01.T01 MTR_2g099550 75.706 177 43 0 6 182 12 188 1.59e-77 229
MsG0880042436.01.T01 MTR_1g081230 55.676 185 79 3 1 182 1 185 1.92e-68 206
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042436.01.T01 AT1G72750 70.968 186 49 2 2 182 3 188 1.46e-81 240
MsG0880042436.01.T01 AT1G17530 67.213 183 56 2 2 182 7 187 3.79e-78 231
MsG0880042436.01.T01 AT1G17530 67.598 179 54 2 2 178 7 183 1.30e-76 227
MsG0880042436.01.T01 AT3G04800 54.237 177 80 1 7 182 12 188 4.03e-53 168

Find 74 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACCGGGTTGGGATTAT+TGG 0.206357 8:-8678483 MsG0880042436.01.T01:CDS
TTGGGAGTTTGTAGAGGTTT+TGG 0.238988 8:+8678724 None:intergenic
CTGGACATTCGGGTCGGACT+TGG 0.241871 8:-8678506 MsG0880042436.01.T01:CDS
GTGGCGGCAGGGCTTGCGAC+TGG 0.268063 8:-8678393 MsG0880042436.01.T01:CDS
TTTGAATGCGTCTGGACATT+CGG 0.314203 8:-8678517 MsG0880042436.01.T01:CDS
GCTGTTGGTGGGGTTGTTGT+TGG 0.333786 8:-8678321 MsG0880042436.01.T01:CDS
GGAACCGGGTTGGGATTATT+GGG 0.335103 8:-8678482 MsG0880042436.01.T01:CDS
TTGAATGCGTCTGGACATTC+GGG 0.335254 8:-8678516 MsG0880042436.01.T01:CDS
CCAAACGTAAACGCCGTTCT+TGG 0.336189 8:-8678671 MsG0880042436.01.T01:CDS
TGGACATTCGGGTCGGACTT+GGG 0.342678 8:-8678505 MsG0880042436.01.T01:CDS
CCAAGAACGGCGTTTACGTT+TGG 0.359864 8:+8678671 None:intergenic
TGGCGGCAGGGCTTGCGACT+GGG 0.360637 8:-8678392 MsG0880042436.01.T01:CDS
CTTTGTATGGGTTGTAGAAT+CGG 0.367000 8:+8678763 None:intergenic
GTGAGATCGGCTGCAGTTGC+TGG 0.372108 8:-8678345 MsG0880042436.01.T01:CDS
CAGTTGCTGGTGCTGTTGGT+GGG 0.374620 8:-8678332 MsG0880042436.01.T01:CDS
GCAGTTGCTGGTGCTGTTGG+TGG 0.383273 8:-8678333 MsG0880042436.01.T01:CDS
CTCACCCTCACACTTCAAAT+AGG 0.389316 8:+8678258 None:intergenic
CAAACGTAAACGCCGTTCTT+GGG 0.395053 8:-8678670 MsG0880042436.01.T01:CDS
ATTATTGGGTTGTTGTATGC+TGG 0.396327 8:-8678468 MsG0880042436.01.T01:CDS
CAACCGACACTGCGAAGCTT+AGG 0.410403 8:-8678551 MsG0880042436.01.T01:CDS
GGACATTCGGGTCGGACTTG+GGG 0.423980 8:-8678504 MsG0880042436.01.T01:CDS
CGCAGTGTCGGTTGATTCAA+AGG 0.434906 8:+8678560 None:intergenic
GGAACTTCGAGGTCTTTGTA+TGG 0.447847 8:+8678750 None:intergenic
GGTGGTTCAATCGCCGGTGC+CGG 0.452433 8:-8678609 MsG0880042436.01.T01:CDS
GCTGCAGTTGCTGGTGCTGT+TGG 0.455549 8:-8678336 MsG0880042436.01.T01:CDS
ACCACCGAGGTAACCGCAAC+CGG 0.465973 8:+8678626 None:intergenic
AGCTGCTGTGGCTGCGAAGA+GGG 0.467196 8:-8678298 MsG0880042436.01.T01:CDS
AAGAGATACTCCGGTGAGGT+TGG 0.473161 8:+8678705 None:intergenic
TTTGTATGGGTTGTAGAATC+GGG 0.474283 8:+8678764 None:intergenic
AGAGATACTCCGGTGAGGTT+GGG 0.474381 8:+8678706 None:intergenic
TACCTCGGTGGTTCAATCGC+CGG 0.491792 8:-8678615 MsG0880042436.01.T01:CDS
CTGCGAAGCTTAGGGTGAAT+CGG 0.491985 8:-8678542 MsG0880042436.01.T01:CDS
CGGTTTATCGAGCTGCGAGA+GGG 0.492635 8:-8678368 MsG0880042436.01.T01:CDS
GCCGGAGTCGGACTCGTTGA+AGG 0.494032 8:-8678591 MsG0880042436.01.T01:CDS
GGGTTGTAGAATCGGGTTTG+AGG 0.496826 8:+8678771 None:intergenic
TCAATCGCCGGTGCCGGAGT+CGG 0.501515 8:-8678603 MsG0880042436.01.T01:CDS
AGTTGCTGGTGCTGTTGGTG+GGG 0.504206 8:-8678331 MsG0880042436.01.T01:CDS
AACGAGTCCGACTCCGGCAC+CGG 0.506992 8:+8678596 None:intergenic
GAACTTCGAGGTCTTTGTAT+GGG 0.509711 8:+8678751 None:intergenic
AACCGACACTGCGAAGCTTA+GGG 0.515795 8:-8678550 MsG0880042436.01.T01:CDS
CACCCTAAGCTTCGCAGTGT+CGG 0.516235 8:+8678548 None:intergenic
TGAGGGATGTTGATGATGCT+TGG 0.516589 8:-8678422 MsG0880042436.01.T01:CDS
GGTTGTTGTTGGAGCTGCTG+TGG 0.524367 8:-8678310 MsG0880042436.01.T01:CDS
TAAGATTCTCGCCCCAAGAA+CGG 0.528444 8:+8678658 None:intergenic
AGCTGCGAGAGGGGTGAGAT+CGG 0.529491 8:-8678358 MsG0880042436.01.T01:CDS
GGCGGCAGGGCTTGCGACTG+GGG 0.534649 8:-8678391 MsG0880042436.01.T01:CDS
GCTTGGAATAGTGTGGCGGC+AGG 0.535474 8:-8678405 MsG0880042436.01.T01:CDS
TACACCGGTTGCGGTTACCT+CGG 0.537617 8:-8678630 MsG0880042436.01.T01:CDS
GGCAGGGCTTGCGACTGGGG+CGG 0.541437 8:-8678388 MsG0880042436.01.T01:CDS
GAGCTGCTGTGGCTGCGAAG+AGG 0.542881 8:-8678299 MsG0880042436.01.T01:CDS
GACATTCGGGTCGGACTTGG+GGG 0.553800 8:-8678503 MsG0880042436.01.T01:CDS
ACCTTCAACGAGTCCGACTC+CGG 0.555318 8:+8678590 None:intergenic
TGCTGTGGCTGCGAAGAGGG+TGG 0.556825 8:-8678295 MsG0880042436.01.T01:CDS
TTGTATGCTGGAATTGAGAG+TGG 0.564300 8:-8678456 MsG0880042436.01.T01:CDS
GTGAGGTTGGGAGTTTGTAG+AGG 0.573117 8:+8678718 None:intergenic
TCGGGTTTGAGGATCTTGAT+CGG 0.578030 8:+8678782 None:intergenic
GAGTGGGATTGAGGCTGTGA+GGG 0.578683 8:-8678439 MsG0880042436.01.T01:CDS
GCGGTTTATCGAGCTGCGAG+AGG 0.585312 8:-8678369 MsG0880042436.01.T01:CDS
AAACGTAAACGCCGTTCTTG+GGG 0.585932 8:-8678669 MsG0880042436.01.T01:CDS
CTACAAACTCCCAACCTCAC+CGG 0.586121 8:-8678715 MsG0880042436.01.T01:CDS
CTTGGAATAGTGTGGCGGCA+GGG 0.601724 8:-8678404 MsG0880042436.01.T01:CDS
TGGAATTGAGAGTGGGATTG+AGG 0.602728 8:-8678448 MsG0880042436.01.T01:CDS
TGTATGCTGGAATTGAGAGT+GGG 0.606585 8:-8678455 MsG0880042436.01.T01:CDS
ACCGGTTGCGGTTACCTCGG+TGG 0.616170 8:-8678627 MsG0880042436.01.T01:CDS
TGTCGGTTGATTCAAAGGAT+CGG 0.630967 8:+8678565 None:intergenic
GGTTTATCGAGCTGCGAGAG+GGG 0.643337 8:-8678367 MsG0880042436.01.T01:CDS
ATGCGTCTGGACATTCGGGT+CGG 0.644981 8:-8678512 MsG0880042436.01.T01:CDS
TCTTCGTCGAAGAGATACTC+CGG 0.653572 8:+8678696 None:intergenic
AGAGTGGGATTGAGGCTGTG+AGG 0.684010 8:-8678440 MsG0880042436.01.T01:CDS
ACAACCCAATAATCCCAACC+CGG 0.701053 8:+8678478 None:intergenic
TGATGATGCTTGGAATAGTG+TGG 0.705715 8:-8678412 MsG0880042436.01.T01:CDS
GTCGAAGAGATACTCCGGTG+AGG 0.727648 8:+8678701 None:intergenic
TGATGCTTGGAATAGTGTGG+CGG 0.746290 8:-8678409 MsG0880042436.01.T01:CDS
CACCGGCGATTGAACCACCG+AGG 0.760521 8:+8678613 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAGAATCTTACTTTTTACAC+CGG - Chr8:8678425-8678444 MsG0880042436.01.T01:CDS 30.0%
! ATTATTGGGTTGTTGTATGC+TGG - Chr8:8678602-8678621 MsG0880042436.01.T01:CDS 35.0%
! CTTTGTATGGGTTGTAGAAT+CGG + Chr8:8678310-8678329 None:intergenic 35.0%
! TTTGTATGGGTTGTAGAATC+GGG + Chr8:8678309-8678328 None:intergenic 35.0%
!!! AGTTTGTAGAGGTTTTGGAT+TGG + Chr8:8678344-8678363 None:intergenic 35.0%
GAACTTCGAGGTCTTTGTAT+GGG + Chr8:8678322-8678341 None:intergenic 40.0%
TGTATGCTGGAATTGAGAGT+GGG - Chr8:8678615-8678634 MsG0880042436.01.T01:CDS 40.0%
TTGTATGCTGGAATTGAGAG+TGG - Chr8:8678614-8678633 MsG0880042436.01.T01:CDS 40.0%
TTTGAATGCGTCTGGACATT+CGG - Chr8:8678553-8678572 MsG0880042436.01.T01:CDS 40.0%
! AATCGGATTTTGAATGCGTC+TGG - Chr8:8678545-8678564 MsG0880042436.01.T01:CDS 40.0%
! TGATGATGCTTGGAATAGTG+TGG - Chr8:8678658-8678677 MsG0880042436.01.T01:CDS 40.0%
! TGTCGGTTGATTCAAAGGAT+CGG + Chr8:8678508-8678527 None:intergenic 40.0%
! TTGGGAGTTTGTAGAGGTTT+TGG + Chr8:8678349-8678368 None:intergenic 40.0%
!!! CTTACTTTTTACACCGGTTG+CGG - Chr8:8678431-8678450 MsG0880042436.01.T01:CDS 40.0%
AAACGTAAACGCCGTTCTTG+GGG - Chr8:8678401-8678420 MsG0880042436.01.T01:CDS 45.0%
ACAACCCAATAATCCCAACC+CGG + Chr8:8678595-8678614 None:intergenic 45.0%
CAAACGTAAACGCCGTTCTT+GGG - Chr8:8678400-8678419 MsG0880042436.01.T01:CDS 45.0%
GGAACTTCGAGGTCTTTGTA+TGG + Chr8:8678323-8678342 None:intergenic 45.0%
TAAGATTCTCGCCCCAAGAA+CGG + Chr8:8678415-8678434 None:intergenic 45.0%
TCGGGTTTGAGGATCTTGAT+CGG + Chr8:8678291-8678310 None:intergenic 45.0%
TCTTCGTCGAAGAGATACTC+CGG + Chr8:8678377-8678396 None:intergenic 45.0%
TGATGCTTGGAATAGTGTGG+CGG - Chr8:8678661-8678680 MsG0880042436.01.T01:CDS 45.0%
TGGAATTGAGAGTGGGATTG+AGG - Chr8:8678622-8678641 MsG0880042436.01.T01:CDS 45.0%
TTGAATGCGTCTGGACATTC+GGG - Chr8:8678554-8678573 MsG0880042436.01.T01:CDS 45.0%
!! TGAGGGATGTTGATGATGCT+TGG - Chr8:8678648-8678667 MsG0880042436.01.T01:CDS 45.0%
!!! GGTTTTGGATTGGAACTTCG+AGG + Chr8:8678334-8678353 None:intergenic 45.0%
AACCGACACTGCGAAGCTTA+GGG - Chr8:8678520-8678539 MsG0880042436.01.T01:CDS 50.0%
AAGAGATACTCCGGTGAGGT+TGG + Chr8:8678368-8678387 None:intergenic 50.0%
AGAGATACTCCGGTGAGGTT+GGG + Chr8:8678367-8678386 None:intergenic 50.0%
CCAAACGTAAACGCCGTTCT+TGG - Chr8:8678399-8678418 MsG0880042436.01.T01:CDS 50.0%
CCAAGAACGGCGTTTACGTT+TGG + Chr8:8678402-8678421 None:intergenic 50.0%
CTACAAACTCCCAACCTCAC+CGG - Chr8:8678355-8678374 MsG0880042436.01.T01:CDS 50.0%
CTGCGAAGCTTAGGGTGAAT+CGG - Chr8:8678528-8678547 MsG0880042436.01.T01:CDS 50.0%
GGGTTGTAGAATCGGGTTTG+AGG + Chr8:8678302-8678321 None:intergenic 50.0%
GTGAGGTTGGGAGTTTGTAG+AGG + Chr8:8678355-8678374 None:intergenic 50.0%
! GGAACCGGGTTGGGATTATT+GGG - Chr8:8678588-8678607 MsG0880042436.01.T01:CDS 50.0%
!! CGCAGTGTCGGTTGATTCAA+AGG + Chr8:8678513-8678532 None:intergenic 50.0%
ACCTTCAACGAGTCCGACTC+CGG + Chr8:8678483-8678502 None:intergenic 55.0%
AGAGTGGGATTGAGGCTGTG+AGG - Chr8:8678630-8678649 MsG0880042436.01.T01:CDS 55.0%
ATGCGTCTGGACATTCGGGT+CGG - Chr8:8678558-8678577 MsG0880042436.01.T01:CDS 55.0%
CAACCGACACTGCGAAGCTT+AGG - Chr8:8678519-8678538 MsG0880042436.01.T01:CDS 55.0%
CACCCTAAGCTTCGCAGTGT+CGG + Chr8:8678525-8678544 None:intergenic 55.0%
CGGTTTATCGAGCTGCGAGA+GGG - Chr8:8678702-8678721 MsG0880042436.01.T01:CDS 55.0%
CTTGGAATAGTGTGGCGGCA+GGG - Chr8:8678666-8678685 MsG0880042436.01.T01:CDS 55.0%
GAGTGGGATTGAGGCTGTGA+GGG - Chr8:8678631-8678650 MsG0880042436.01.T01:CDS 55.0%
GGTTTATCGAGCTGCGAGAG+GGG - Chr8:8678703-8678722 MsG0880042436.01.T01:CDS 55.0%
GTCGAAGAGATACTCCGGTG+AGG + Chr8:8678372-8678391 None:intergenic 55.0%
TACCTCGGTGGTTCAATCGC+CGG - Chr8:8678455-8678474 MsG0880042436.01.T01:CDS 55.0%
TGGACATTCGGGTCGGACTT+GGG - Chr8:8678565-8678584 MsG0880042436.01.T01:CDS 55.0%
! GCTGTTGGTGGGGTTGTTGT+TGG - Chr8:8678749-8678768 MsG0880042436.01.T01:CDS 55.0%
! GGGAACCGGGTTGGGATTAT+TGG - Chr8:8678587-8678606 MsG0880042436.01.T01:CDS 55.0%
! GGTTGTTGTTGGAGCTGCTG+TGG - Chr8:8678760-8678779 MsG0880042436.01.T01:CDS 55.0%
! TACACCGGTTGCGGTTACCT+CGG - Chr8:8678440-8678459 MsG0880042436.01.T01:CDS 55.0%
!! AGTTGCTGGTGCTGTTGGTG+GGG - Chr8:8678739-8678758 MsG0880042436.01.T01:CDS 55.0%
!! CAGTTGCTGGTGCTGTTGGT+GGG - Chr8:8678738-8678757 MsG0880042436.01.T01:CDS 55.0%
ACCACCGAGGTAACCGCAAC+CGG + Chr8:8678447-8678466 None:intergenic 60.0%
AGCTGCGAGAGGGGTGAGAT+CGG - Chr8:8678712-8678731 MsG0880042436.01.T01:CDS 60.0%
AGCTGCTGTGGCTGCGAAGA+GGG - Chr8:8678772-8678791 MsG0880042436.01.T01:CDS 60.0%
CTGGACATTCGGGTCGGACT+TGG - Chr8:8678564-8678583 MsG0880042436.01.T01:CDS 60.0%
GACATTCGGGTCGGACTTGG+GGG - Chr8:8678567-8678586 MsG0880042436.01.T01:CDS 60.0%
GCGGTTTATCGAGCTGCGAG+AGG - Chr8:8678701-8678720 MsG0880042436.01.T01:CDS 60.0%
GCTTGGAATAGTGTGGCGGC+AGG - Chr8:8678665-8678684 MsG0880042436.01.T01:CDS 60.0%
GGACATTCGGGTCGGACTTG+GGG - Chr8:8678566-8678585 MsG0880042436.01.T01:CDS 60.0%
GTGAGATCGGCTGCAGTTGC+TGG - Chr8:8678725-8678744 MsG0880042436.01.T01:CDS 60.0%
!! GCAGTTGCTGGTGCTGTTGG+TGG - Chr8:8678737-8678756 MsG0880042436.01.T01:CDS 60.0%
!! GCTGCAGTTGCTGGTGCTGT+TGG - Chr8:8678734-8678753 MsG0880042436.01.T01:CDS 60.0%
AACGAGTCCGACTCCGGCAC+CGG + Chr8:8678477-8678496 None:intergenic 65.0%
ACCGGTTGCGGTTACCTCGG+TGG - Chr8:8678443-8678462 MsG0880042436.01.T01:CDS 65.0%
CACCGGCGATTGAACCACCG+AGG + Chr8:8678460-8678479 None:intergenic 65.0%
GAGCTGCTGTGGCTGCGAAG+AGG - Chr8:8678771-8678790 MsG0880042436.01.T01:CDS 65.0%
GCCGGAGTCGGACTCGTTGA+AGG - Chr8:8678479-8678498 MsG0880042436.01.T01:CDS 65.0%
GGACTTGGGGGAACCGGGTT+GGG - Chr8:8678579-8678598 MsG0880042436.01.T01:CDS 65.0%
GGTGGTTCAATCGCCGGTGC+CGG - Chr8:8678461-8678480 MsG0880042436.01.T01:CDS 65.0%
TGCTGTGGCTGCGAAGAGGG+TGG - Chr8:8678775-8678794 MsG0880042436.01.T01:CDS 65.0%
! TCAATCGCCGGTGCCGGAGT+CGG - Chr8:8678467-8678486 MsG0880042436.01.T01:CDS 65.0%
CGGACTTGGGGGAACCGGGT+TGG - Chr8:8678578-8678597 MsG0880042436.01.T01:CDS 70.0%
CGGGTCGGACTTGGGGGAAC+CGG - Chr8:8678573-8678592 MsG0880042436.01.T01:CDS 70.0%
GGGTCGGACTTGGGGGAACC+GGG - Chr8:8678574-8678593 MsG0880042436.01.T01:CDS 70.0%
TGGCGGCAGGGCTTGCGACT+GGG - Chr8:8678678-8678697 MsG0880042436.01.T01:CDS 70.0%
GGCAGGGCTTGCGACTGGGG+CGG - Chr8:8678682-8678701 MsG0880042436.01.T01:CDS 75.0%
GGCGGCAGGGCTTGCGACTG+GGG - Chr8:8678679-8678698 MsG0880042436.01.T01:CDS 75.0%
GTGGCGGCAGGGCTTGCGAC+TGG - Chr8:8678677-8678696 MsG0880042436.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr8 gene 8678272 8678820 8678272 ID=MsG0880042436.01;Name=MsG0880042436.01
Chr8 mRNA 8678272 8678820 8678272 ID=MsG0880042436.01.T01;Parent=MsG0880042436.01;Name=MsG0880042436.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|182
Chr8 exon 8678272 8678820 8678272 ID=MsG0880042436.01.T01:exon:4052;Parent=MsG0880042436.01.T01
Chr8 CDS 8678272 8678820 8678272 ID=MsG0880042436.01.T01:cds;Parent=MsG0880042436.01.T01
Gene Sequence

>MsG0880042436.01.T01

ATGGCGAATAACGAATCCGATCAAGATCCTCAAACCCGATTCTACAACCCATACAAAGACCTCGAAGTTCCAATCCAAAACCTCTACAAACTCCCAACCTCACCGGAGTATCTCTTCGACGAAGAAGCCAAACGTAAACGCCGTTCTTGGGGCGAGAATCTTACTTTTTACACCGGTTGCGGTTACCTCGGTGGTTCAATCGCCGGTGCCGGAGTCGGACTCGTTGAAGGTATCCGATCCTTTGAATCAACCGACACTGCGAAGCTTAGGGTGAATCGGATTTTGAATGCGTCTGGACATTCGGGTCGGACTTGGGGGAACCGGGTTGGGATTATTGGGTTGTTGTATGCTGGAATTGAGAGTGGGATTGAGGCTGTGAGGGATGTTGATGATGCTTGGAATAGTGTGGCGGCAGGGCTTGCGACTGGGGCGGTTTATCGAGCTGCGAGAGGGGTGAGATCGGCTGCAGTTGCTGGTGCTGTTGGTGGGGTTGTTGTTGGAGCTGCTGTGGCTGCGAAGAGGGTGGCGAAACGATACGTGCCTATTTGA

Protein sequence

>MsG0880042436.01.T01

MANNESDQDPQTRFYNPYKDLEVPIQNLYKLPTSPEYLFDEEAKRKRRSWGENLTFYTGCGYLGGSIAGAGVGLVEGIRSFESTDTAKLRVNRILNASGHSGRTWGNRVGIIGLLYAGIESGIEAVRDVDDAWNSVAAGLATGAVYRAARGVRSAAVAGAVGGVVVGAAVAAKRVAKRYVPI*