AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010205.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010205.01.T01 MTR_1g092430 85.437 103 10 1 1 103 1 98 4.54e-45 141
MsG0280010205.01.T01 MTR_0271s0050 74.038 104 11 2 1 103 1 89 5.81e-39 125
MsG0280010205.01.T01 MTR_0648s0010 74.038 104 11 2 1 103 1 89 5.81e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010205.01.T01 AT3G15352 88.889 45 5 0 59 103 53 97 6.46e-27 95.5
MsG0280010205.01.T01 AT3G15352 88.889 45 5 0 59 103 31 75 6.84e-27 94.7
MsG0280010205.01.T01 AT3G15352 88.889 45 5 0 59 103 30 74 7.59e-27 94.4
MsG0280010205.01.T01 AT3G15352 88.889 45 5 0 59 103 37 81 1.12e-26 94.4
MsG0280010205.01.T01 AT1G53030 82.222 45 8 0 59 103 28 72 1.71e-24 88.6

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGCGTTGCTGCAGTTGC+TGG 0.322432 2:-66167144 None:intergenic
GCTGGCGTTGCTGCTGTTGA+TGG 0.363493 2:-66167171 None:intergenic
ACCAGCAACTGCAGCAACGC+CGG 0.403671 2:+66167143 MsG0280010205.01.T01:CDS
AACGCCAGCGACGGCAGAAA+CGG 0.425755 2:+66167185 MsG0280010205.01.T01:CDS
TCTCGCAGCCTCTTCGTGTC+AGG 0.437710 2:-66167273 None:intergenic
CAGTTGCTGGTGCTGTTGGT+TGG 0.439790 2:-66167131 None:intergenic
CGAATTTGTCTTCGTGCTGA+AGG 0.441593 2:+66167351 MsG0280010205.01.T01:CDS
GCCGCCGTTTCTGCCGTCGC+TGG 0.445253 2:-66167189 None:intergenic
GCTGCTGTTGATGGCGCTGC+CGG 0.473729 2:-66167162 None:intergenic
GCTGCAGTTGCTGGTGCTGT+TGG 0.489129 2:-66167135 None:intergenic
AACAGCAGCAACGCCAGCGA+CGG 0.494709 2:+66167176 MsG0280010205.01.T01:CDS
TTTGACACTTCACATGCTGC+TGG 0.518991 2:-66167216 None:intergenic
GCCAGCGACGGCAGAAACGG+CGG 0.552546 2:+66167188 MsG0280010205.01.T01:CDS
TGGTTCGAAGCAGAGGAAGA+AGG 0.559341 2:-66167111 None:intergenic
TGTTGGTTGGTTCGAAGCAG+AGG 0.577421 2:-66167118 None:intergenic
GGTAACGCGCAAGTGCAAAA+CGG 0.577765 2:+66167078 MsG0280010205.01.T01:CDS
ATGAGTGCATAGTGGAACAC+GGG 0.582328 2:+66167295 MsG0280010205.01.T01:CDS
TCGATGAGCTTCAATCCACT+TGG 0.585020 2:-66167331 None:intergenic
GCGCTTGTCCTGACACGAAG+AGG 0.611666 2:+66167265 MsG0280010205.01.T01:CDS
GCTGCGAGATGAGTGCATAG+TGG 0.621172 2:+66167287 MsG0280010205.01.T01:CDS
GGGAGGATGCTTGTGCCAAG+TGG 0.626172 2:+66167316 MsG0280010205.01.T01:CDS
GATGAGTGCATAGTGGAACA+CGG 0.674556 2:+66167294 MsG0280010205.01.T01:CDS
GTGCATAGTGGAACACGGGG+AGG 0.723863 2:+66167299 MsG0280010205.01.T01:CDS
TGAGTGCATAGTGGAACACG+GGG 0.769217 2:+66167296 MsG0280010205.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAACAGATTTTCTTCTTCTT+CGG - Chr2:66167246-66167265 None:intergenic 30.0%
CGAATTTGTCTTCGTGCTGA+AGG + Chr2:66167351-66167370 MsG0280010205.01.T01:CDS 45.0%
TCGATGAGCTTCAATCCACT+TGG - Chr2:66167334-66167353 None:intergenic 45.0%
TTTGACACTTCACATGCTGC+TGG - Chr2:66167219-66167238 None:intergenic 45.0%
! ATGAGTGCATAGTGGAACAC+GGG + Chr2:66167295-66167314 MsG0280010205.01.T01:CDS 45.0%
! GATGAGTGCATAGTGGAACA+CGG + Chr2:66167294-66167313 MsG0280010205.01.T01:CDS 45.0%
GGTAACGCGCAAGTGCAAAA+CGG + Chr2:66167078-66167097 MsG0280010205.01.T01:CDS 50.0%
TGGTTCGAAGCAGAGGAAGA+AGG - Chr2:66167114-66167133 None:intergenic 50.0%
! TGAGTGCATAGTGGAACACG+GGG + Chr2:66167296-66167315 MsG0280010205.01.T01:CDS 50.0%
!! TGTTGGTTGGTTCGAAGCAG+AGG - Chr2:66167121-66167140 None:intergenic 50.0%
GCTGCGAGATGAGTGCATAG+TGG + Chr2:66167287-66167306 MsG0280010205.01.T01:CDS 55.0%
!! CAGTTGCTGGTGCTGTTGGT+TGG - Chr2:66167134-66167153 None:intergenic 55.0%
AACAGCAGCAACGCCAGCGA+CGG + Chr2:66167176-66167195 MsG0280010205.01.T01:CDS 60.0%
AACGCCAGCGACGGCAGAAA+CGG + Chr2:66167185-66167204 MsG0280010205.01.T01:CDS 60.0%
ACCAGCAACTGCAGCAACGC+CGG + Chr2:66167143-66167162 MsG0280010205.01.T01:CDS 60.0%
GCGCTTGTCCTGACACGAAG+AGG + Chr2:66167265-66167284 MsG0280010205.01.T01:CDS 60.0%
GCTGGCGTTGCTGCTGTTGA+TGG - Chr2:66167174-66167193 None:intergenic 60.0%
GGGAGGATGCTTGTGCCAAG+TGG + Chr2:66167316-66167335 MsG0280010205.01.T01:CDS 60.0%
TCTCGCAGCCTCTTCGTGTC+AGG - Chr2:66167276-66167295 None:intergenic 60.0%
! GTGCATAGTGGAACACGGGG+AGG + Chr2:66167299-66167318 MsG0280010205.01.T01:CDS 60.0%
!! GCTGCAGTTGCTGGTGCTGT+TGG - Chr2:66167138-66167157 None:intergenic 60.0%
!! GCTGCTGTTGATGGCGCTGC+CGG - Chr2:66167165-66167184 None:intergenic 65.0%
GCCAGCGACGGCAGAAACGG+CGG + Chr2:66167188-66167207 MsG0280010205.01.T01:CDS 70.0%
GCCGGCGTTGCTGCAGTTGC+TGG - Chr2:66167147-66167166 None:intergenic 70.0%
GCCGCCGTTTCTGCCGTCGC+TGG - Chr2:66167192-66167211 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 66167075 66167386 66167075 ID=MsG0280010205.01;Name=MsG0280010205.01
Chr2 mRNA 66167075 66167386 66167075 ID=MsG0280010205.01.T01;Parent=MsG0280010205.01;Name=MsG0280010205.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|103
Chr2 exon 66167075 66167386 66167075 ID=MsG0280010205.01.T01:exon:14250;Parent=MsG0280010205.01.T01
Chr2 CDS 66167075 66167386 66167075 ID=MsG0280010205.01.T01:cds;Parent=MsG0280010205.01.T01
Gene Sequence

>MsG0280010205.01.T01

ATGGGTAACGCGCAAGTGCAAAACGGTGTTGCTGTTCCTTCTTCCTCTGCTTCGAACCAACCAACAGCACCAGCAACTGCAGCAACGCCGGCAGCGCCATCAACAGCAGCAACGCCAGCGACGGCAGAAACGGCGGCAACGCCAGCAGCATGTGAAGTGTCAAATGCTCCGAAGAAGAAGAAAATCTGTTGCGCTTGTCCTGACACGAAGAGGCTGCGAGATGAGTGCATAGTGGAACACGGGGAGGATGCTTGTGCCAAGTGGATTGAAGCTCATCGAATTTGTCTTCGTGCTGAAGGATTCAATGTTTGA

Protein sequence

>MsG0280010205.01.T01

MGNAQVQNGVAVPSSSASNQPTAPATAATPAAPSTAATPATAETAATPAACEVSNAPKKKKICCACPDTKRLRDECIVEHGEDACAKWIEAHRICLRAEGFNV*