AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029717.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029717.01.T02 MTR_5g091310 95.238 63 3 0 1 63 24 86 6.11e-40 126
MsG0580029717.01.T02 MTR_4g094630 50.769 65 26 2 4 63 11 74 9.63e-16 64.7
MsG0580029717.01.T02 MTR_4g094635 51.562 64 26 2 4 63 28 90 2.38e-15 63.9
MsG0580029717.01.T01 MTR_5g091310 95.349 86 4 0 1 86 1 86 4.71e-56 167
MsG0580029717.01.T01 MTR_4g094635 50.000 74 30 3 17 86 20 90 9.61e-17 68.6
MsG0580029717.01.T01 MTR_4g094630 48.000 75 31 3 17 86 3 74 1.01e-15 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029717.01.T02 AT5G46295 58.140 43 17 1 21 63 30 71 8.88e-13 57.0
MsG0580029717.01.T02 AT1G06475 56.818 44 18 1 20 62 49 92 6.62e-12 55.5
MsG0580029717.01.T01 AT5G46295 58.140 43 17 1 44 86 30 71 1.27e-12 57.8
MsG0580029717.01.T01 AT1G06475 56.818 44 18 1 43 85 49 92 3.26e-12 57.4

Find 12 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGTGAAGAAAGAAATT+TGG 0.267366 5:-101115914 MsG0580029717.01.T01:CDS
CATGGAGATTCTCTGCGTTT+AGG 0.350104 5:-101115890 MsG0580029717.01.T01:CDS
TCGTGGATACTGTTGTGTTT+AGG 0.353121 5:-101115836 MsG0580029717.01.T01:CDS
TAGTTTGAGAAAGCGTTTGA+TGG 0.413544 5:-101115964 MsG0580029717.01.T01:CDS
GAAAGAAGAGTAGCATGTTA+TGG 0.462290 5:-101115941 MsG0580029717.01.T01:CDS
TCATCTTCAAATAACATTCG+TGG 0.467647 5:-101115853 MsG0580029717.01.T01:CDS
TGCTTCTTCTATCTTTGTTG+TGG 0.470772 5:-101115771 MsG0580029717.01.T01:CDS
AGTTTGAGAAAGCGTTTGAT+GGG 0.536928 5:-101115963 MsG0580029717.01.T01:CDS
GGAGATTCTCTGCGTTTAGG+TGG 0.556332 5:-101115887 MsG0580029717.01.T01:CDS
TGAAGAAAGAAATTTGGCCA+TGG 0.584343 5:-101115908 MsG0580029717.01.T01:CDS
AAGATAGAAGAAGCAAACAG+TGG 0.638854 5:+101115779 None:intergenic
CTAAACGCAGAGAATCTCCA+TGG 0.659319 5:+101115891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTGTAATATCTTTTGTT+TGG + Chr5:101115769-101115788 None:intergenic 20.0%
! AGAAAGTGAAGAAAGAAATT+TGG - Chr5:101115840-101115859 MsG0580029717.01.T01:CDS 25.0%
TCATCTTCAAATAACATTCG+TGG - Chr5:101115901-101115920 MsG0580029717.01.T01:CDS 30.0%
AAGATAGAAGAAGCAAACAG+TGG + Chr5:101115978-101115997 None:intergenic 35.0%
GAAAGAAGAGTAGCATGTTA+TGG - Chr5:101115813-101115832 MsG0580029717.01.T01:CDS 35.0%
TGAAGAAAGAAATTTGGCCA+TGG - Chr5:101115846-101115865 MsG0580029717.01.T01:CDS 35.0%
TGCTTCTTCTATCTTTGTTG+TGG - Chr5:101115983-101116002 MsG0580029717.01.T01:CDS 35.0%
!! AGTTTGAGAAAGCGTTTGAT+GGG - Chr5:101115791-101115810 MsG0580029717.01.T01:CDS 35.0%
!! TAGTTTGAGAAAGCGTTTGA+TGG - Chr5:101115790-101115809 MsG0580029717.01.T01:CDS 35.0%
TCGTGGATACTGTTGTGTTT+AGG - Chr5:101115918-101115937 MsG0580029717.01.T01:CDS 40.0%
CATGGAGATTCTCTGCGTTT+AGG - Chr5:101115864-101115883 MsG0580029717.01.T01:CDS 45.0%
CTAAACGCAGAGAATCTCCA+TGG + Chr5:101115866-101115885 None:intergenic 45.0%
GGAGATTCTCTGCGTTTAGG+TGG - Chr5:101115867-101115886 MsG0580029717.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 101115758 101116018 101115758 ID=MsG0580029717.01;Name=MsG0580029717.01
Chr5 mRNA 101115758 101116018 101115758 ID=MsG0580029717.01.T01;Parent=MsG0580029717.01;Name=MsG0580029717.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|86
Chr5 exon 101115758 101116018 101115758 ID=MsG0580029717.01.T01:exon:36746;Parent=MsG0580029717.01.T01
Chr5 CDS 101115758 101116018 101115758 ID=MsG0580029717.01.T01:cds;Parent=MsG0580029717.01.T01
Chr5 mRNA 101115758 101115949 101115758 ID=MsG0580029717.01.T02;Parent=MsG0580029717.01;Name=MsG0580029717.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|63
Chr5 exon 101115758 101115949 101115758 ID=MsG0580029717.01.T02:exon:36747;Parent=MsG0580029717.01.T02
Chr5 CDS 101115758 101115949 101115758 ID=MsG0580029717.01.T02:cds;Parent=MsG0580029717.01.T02
Chr5 mRNA 101115891 101115911 101115891 ID=MsG0580029717.01.T03;Parent=MsG0580029717.01;Name=MsG0580029717.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|6
Chr5 exon 101115891 101115911 101115891 ID=MsG0580029717.01.T03:exon:36748;Parent=MsG0580029717.01.T03
Chr5 CDS 101115891 101115911 101115891 ID=MsG0580029717.01.T03:cds;Parent=MsG0580029717.01.T03
Gene Sequence

>MsG0580029717.01.T02

ATGTTATGGTTGAAGAAAGTGAAGAAAGAAATTTGGCCATGGAGATTCTCTGCGTTTAGGTGGAAACGACTTGATCATCTTCAAATAACATTCGTGGATACTGTTGTGTTTAGGATACTCTCTGTTGCTGAAGCTGTTGTTCTTGTCTCCACTGTTTGCTTCTTCTATCTTTGTTGTGGCTGCAATTTCTGA

>MsG0580029717.01.T03

ATGGAGATTCTCTGCGTTTAG

>MsG0580029717.01.T01

ATGGAAGACCAAACAAAAGATATTACAAAGAGTAGTTTGAGAAAGCGTTTGATGGGAAAGAAGAGTAGCATGTTATGGTTGAAGAAAGTGAAGAAAGAAATTTGGCCATGGAGATTCTCTGCGTTTAGGTGGAAACGACTTGATCATCTTCAAATAACATTCGTGGATACTGTTGTGTTTAGGATACTCTCTGTTGCTGAAGCTGTTGTTCTTGTCTCCACTGTTTGCTTCTTCTATCTTTGTTGTGGCTGCAATTTCTGA

Protein sequence

>MsG0580029717.01.T02

MLWLKKVKKEIWPWRFSAFRWKRLDHLQITFVDTVVFRILSVAEAVVLVSTVCFFYLCCGCNF*

>MsG0580029717.01.T03

MEILCV*

>MsG0580029717.01.T01

MEDQTKDITKSSLRKRLMGKKSSMLWLKKVKKEIWPWRFSAFRWKRLDHLQITFVDTVVFRILSVAEAVVLVSTVCFFYLCCGCNF*