AlfalfaGEDB | Msa0043470

M. sativa cultivar zhongmu-4 / Msa0043470

KEGG: K04733
GO: -
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0043470	sp\|Q9M9C5\|Y1680_ARATH	63.117	648	188	10	201	805	31	670	0.0	770

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001940	Msa0043470	0.812983	3.221730e-51	-8.615850e-47
Msa0005890	Msa0043470	0.814340	1.619557e-51	-8.615850e-47
Msa0017950	Msa0043470	0.804499	2.097668e-49	-8.615850e-47
Msa0028420	Msa0043470	0.807413	5.116037e-50	-8.615850e-47
Msa0043470	Msa0043620	0.869882	2.218563e-66	-8.615850e-47
Msa0043470	Msa0048510	0.818773	1.645675e-52	-8.615850e-47
Msa0043470	Msa0081980	0.803796	2.938438e-49	-8.615850e-47
Msa0043470	Msa0094780	0.818471	1.926898e-52	-8.615850e-47
Msa0043470	Msa0174450	0.817592	3.044203e-52	-8.615850e-47
Msa0043470	Msa0182190	0.851614	7.913536e-61	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0043470	MtrunA17_Chr1g0209521	90.549	656	19	5	189	805	18	669	0.0	1169
Msa0043470	MtrunA17_Chr5g0423121	58.358	670	197	15	174	801	1	630	0.0	728
Msa0043470	MtrunA17_Chr3g0128551	43.333	630	308	16	178	777	8	618	1.22e-150	455
Msa0043470	MtrunA17_Chr1g0179521	42.625	678	309	20	181	800	3	658	9.06e-150	454
Msa0043470	MtrunA17_Chr3g0108301	43.360	625	301	17	183	774	23	627	3.84e-148	449
Msa0043470	MtrunA17_Chr7g0245171	43.014	637	288	22	177	775	9	608	3.40e-138	422
Msa0043470	MtrunA17_Chr5g0432851	42.209	661	287	19	165	774	12	628	4.81e-138	423
Msa0043470	MtrunA17_Chr3g0126271	41.214	626	296	16	183	774	7	594	6.25e-137	419
Msa0043470	MtrunA17_Chr6g0478621	42.219	649	285	21	197	773	18	648	7.14e-132	408
Msa0043470	MtrunA17_Chr5g0428541	40.191	627	304	15	180	778	9	592	3.89e-130	401

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0043470	AT1G68400.1	63.117	648	188	10	201	805	31	670	0.0	770
Msa0043470	AT2G26730.1	43.946	669	282	16	181	793	6	637	7.15e-156	470
Msa0043470	AT2G36570.1	42.097	658	305	17	201	795	24	668	8.32e-149	452
Msa0043470	AT5G58300.3	43.269	624	299	16	183	774	30	630	4.29e-148	449
Msa0043470	AT5G58300.1	43.269	624	299	16	183	774	30	630	4.29e-148	449
Msa0043470	AT5G58300.2	43.269	624	299	16	183	774	30	630	4.29e-148	449
Msa0043470	AT1G48480.1	43.868	636	273	17	198	774	33	643	2.10e-144	440
Msa0043470	AT3G08680.1	40.700	629	331	17	174	774	1	615	8.94e-132	407
Msa0043470	AT3G08680.2	40.700	629	331	17	174	774	1	615	8.94e-132	407
Msa0043470	AT5G05160.1	39.938	641	309	20	183	779	14	622	3.43e-128	397

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Find 221 sgRNAs with CRISPR-Local

Find 1116 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGCTGTCGGGTTTCTC+CGG	0.490726	1_1:+74604808	Msa0043470:CDS
AAAAGTGACGTGACTGTTGA+CGG	0.470711	1_1:-74604595	None:intergenic
AAACCAGGAAGGTTTAGTTC+AGG	0.477328	1_1:-74604739	None:intergenic
AAACGCCGGTCCAAGTGCAT+GGG	0.581768	1_1:-74604372	None:intergenic
AAACTATTCATCAAAATCAA+AGG	0.566234	1_1:+74605116	Msa0043470:CDS
AAAGACATGAATTACCATGA+AGG	0.612051	1_1:-74605530	None:intergenic
AAAGAGTAATAAAGCTATTG+TGG	0.490569	1_1:+74599937	Msa0043470:intron
AAAGATCACCAGGATCAAGA+CGG	0.653333	1_1:-74600039	None:intergenic
AAATCGGATGTCTACTCTTT+TGG	0.288763	1_1:+74606885	Msa0043470:CDS
AAATGGCTACCGTGCTCCTG+AGG	0.614111	1_1:+74606836	Msa0043470:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTAGTATCTTCTCTACAAA+TGG	-	chr1_1:74599888-74599907	None:intergenic	30.0%
GAAGATACTACTACTTTGTA+TGG	+	chr1_1:74599896-74599915	Msa0043470:CDS	30.0%
TACTACTTTGTATGGTGTTT+CGG	+	chr1_1:74599904-74599923	Msa0043470:CDS	30.0%
ATTGTCTCTTGACTAAATGA+TGG	+	chr1_1:74600766-74600785	Msa0043470:intron	30.0%
ATGCAACTGCATAATTTATC+AGG	-	chr1_1:74600983-74601002	None:intergenic	30.0%
AACTCAATTATTTGGTATGG+CGG	-	chr1_1:74601080-74601099	None:intergenic	30.0%
TTCAAAGATAAATTGGGTCA+AGG	+	chr1_1:74601173-74601192	Msa0043470:intron	30.0%
ACTTCTCATGTTAACATTGT+TGG	+	chr1_1:74601317-74601336	Msa0043470:intron	30.0%
ATATATGACTACATGCCTAA+TGG	+	chr1_1:74601380-74601399	Msa0043470:intron	30.0%
AATTGAAGATTGAGCTTCAA+TGG	-	chr1_1:74601440-74601459	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	74599764	74607444	74599764	ID=Msa0043470;Name=Msa0043470
chr1_1	mRNA	74599764	74607444	74599764	ID=Msa0043470-mRNA-1;Parent=Msa0043470;Name=Msa0043470-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|0.42\|0.25\|1\|0.42\|0.25\|8\|164\|805
chr1_1	exon	74599764	74599941	74599764	ID=Msa0043470-mRNA-1:exon:25153;Parent=Msa0043470-mRNA-1
chr1_1	exon	74599951	74600184	74599951	ID=Msa0043470-mRNA-1:exon:25154;Parent=Msa0043470-mRNA-1
chr1_1	exon	74600506	74600541	74600506	ID=Msa0043470-mRNA-1:exon:25155;Parent=Msa0043470-mRNA-1
chr1_1	exon	74600997	74601019	74600997	ID=Msa0043470-mRNA-1:exon:25156;Parent=Msa0043470-mRNA-1
chr1_1	exon	74604166	74605018	74604166	ID=Msa0043470-mRNA-1:exon:25157;Parent=Msa0043470-mRNA-1
chr1_1	exon	74605061	74605243	74605061	ID=Msa0043470-mRNA-1:exon:25158;Parent=Msa0043470-mRNA-1
chr1_1	exon	74605304	74605537	74605304	ID=Msa0043470-mRNA-1:exon:25159;Parent=Msa0043470-mRNA-1
chr1_1	exon	74606604	74607444	74606604	ID=Msa0043470-mRNA-1:exon:25160;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74599764	74599941	74599764	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74599951	74600184	74599951	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74600506	74600541	74600506	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74600997	74601019	74600997	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74604166	74605018	74604166	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74605061	74605243	74605061	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74605304	74605537	74605304	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	CDS	74606604	74607280	74606604	ID=Msa0043470-mRNA-1:cds;Parent=Msa0043470-mRNA-1
chr1_1	three_prime_UTR	74607281	74607444	74607281	ID=Msa0043470-mRNA-1:three_prime_utr;Parent=Msa0043470-mRNA-1

Gene Sequence

>Msa0043470

ATGTTCACGGTCAACACGCCTTTGGTTTCATCTCGTTTGGAATATTACGTAACTGTTTTCGGATGCAACAGCAACAACTCACTTGATCTTCAACAATACTATTCTGTTTCTAATTCTACTTCCATTTGTAGAGAAGATACTACTACTTTGTATGGTGTTTCGGATTCAGATATAAAGACTATTGTGGTTGTAACTGATTCTGTTTCCAATTCTAATCATCTTAAAGGATGTTCAAATGTTGAGCTTCCAACGATTTCAAAGGTTGACCGTCTTGATCCTGGTGATCTTTTTAACGTGTTAAGCGGTGAAATTGCTATCAAATTACAAGTTTCTCAGAATTGTTCTAGCTGCCATGATCTTTATGGTGGTCAATGTCTACTTGATAACAATGGCCAGTTTTATTGTAAACAAGAGAAATCTAAAAAGACCCGTCTTAAAGTGGCAGCAGTTGCATCTTCTGCTGGAGTACTACAACAACGCTCTCACAACGTTATTCTTCTCACTTCTCTCTCTGCAACAATGGCTCCAACACTCTCACCCTTCGTTCTTCTTTTTCTGCTTTCCCATTTTGTCCTCATTGTTCAATCTTCCCTTAACCCAGATTTGGAACCTCTTCTAACCTTCAAAACCGGTTCAGACCAATCTAACAAGCTCTCAACATGGAAAACCAACACCGACCCATGCACTTGGACCGGCGTTTCATGTGTCAAAAACCGAGTCACTCGACTCATTCTCGAAAATCTCAACCTCCAAGGCACCATTGAACCGTTAACCTCACTAACTCAGCTCCGAGTTTTGAGTCTCAAAGGAAACCGCTTTTCCGGTTCACTTCCAAACCTCTCCAACTTCACTTCCCTCAAACTTCTCTTCTTATCTCACAACCATTTCTCCGGCGAGTTTCCGTCAACAGTCACGTCACTTTTCCGTCTCTACCGTCTTGATCTATCCTACAACAATTTCACCGGCGAGATTCCGGCGACGGTCAACCGTTTGACCCATCTTCTTACTCTCCGTCTCGACGAGAATAAATTCTCCGGCGTCATTCCTGAACTAAACCTTCCTGGTTTACAGGACTTCAACGTCTCCTGTAACCGCTTCTCCGGCAAGATACCGAAAACGCTGTCGGGTTTCTCCGGTTCTTCTTTCGGGCAAAACCCGTTTCTATGTGGAGCTCCGTTGGAGAAATGTGGAGATGAACCGAATAAACCTGGATCCGATGGTGCAATTGCTTCACCATTGGTACCAGCAACGGTTGTGTCATCATCTCCCAGCACAATGCCAACAACAACAGCAACACCAACAACAAAAAACCATGAAAAACGAGGTGATGTTCTTGTTCTTGGAATAGTGTGTTTACTTCTATATTGCTATTTTTGGAAAAACTATTCATCAAAATCAAAGGACAAAAAAGGGTTGAAACTATTTGAAAGTGAAAAGATAGTGTATTCATCAAGTCCATACCCTACACAAGGAGGGTTTGAGAGGGGTAGAATGGTATTTTTTGAAGGTGGATTTGGAACTGCTTATAAAGCTGTGCTTGATGATGGAAATGTTGTTGCTGTCAAGAGATTGAAAGATGCACAAATTGCTGGGAAGAGGGAATTTGAACAACATATGGAAATTCTTGGAAGGATTAGACACCCTAATGTCGTTAGTTTAAGGGCTTATTATTTTGCTAGAGATGAGAAGCTTTTGGTTTATGATTACATGCCTAATGCTACTTTGTTCTGGCTCCTTCATGGAAACAGGGGACCTGGAAGAACCCCACTGGATTGGACCACAAGGCTCAAGATAGCAGCTGGAGCTGCACATGGCATAGCATTCATTCACAATTCATGCAAGTCCCTTAAACTCACTCATGGTAACATCAAATCCACCAACATACTCCTTGACAAGCAAGGAGATGCACGCGTATCGGATTTTGGGCTTTCAGTATTCAACGGTTCAAGCCCATCAGGAGCTGGTAGCAGATCAAATGGCTACCGTGCTCCTGAGGTATTGGATGGTCGAAAACAATCCCAAAAATCGGATGTCTACTCTTTTGGTGTTCTGTTGCTAGAGATGCTTACAGGGAAGTGTCCCTCTGCTGTGGAGAGTGGTGGAAGTGGTTATAATGGTGGGGTGATTGATCTACCTAGGTGGGTGCAATCAGTAGTTAGAGAGGAATGGACTGCTGAGGTTTTTGATTTGGAGTTGATGAGGTATAAGGATATTGAAGAGGAAATGGTGGGGTTGCTTCAGATTGCTATGTCTTGTACTGCTGCTTCACCTGATCAACGGCCAAGAATGTCACATGTTGTTAAGATGATTGAGGAATTACGTGGCGTCGAGGTGTCACCCTGTCATGACACGGTGGATTCTGTCTCTGACTCACCTTCTTTGTCTGAAGATGCTTGTGCTGGTACTACTAGTCAGTAA

Protein sequence

>Msa0043470

MFTVNTPLVSSRLEYYVTVFGCNSNNSLDLQQYYSVSNSTSICREDTTTLYGVSDSDIKTIVVVTDSVSNSNHLKGCSNVELPTISKVDRLDPGDLFNVLSGEIAIKLQVSQNCSSCHDLYGGQCLLDNNGQFYCKQEKSKKTRLKVAAVASSAGVLQQRSHNVILLTSLSATMAPTLSPFVLLFLLSHFVLIVQSSLNPDLEPLLTFKTGSDQSNKLSTWKTNTDPCTWTGVSCVKNRVTRLILENLNLQGTIEPLTSLTQLRVLSLKGNRFSGSLPNLSNFTSLKLLFLSHNHFSGEFPSTVTSLFRLYRLDLSYNNFTGEIPATVNRLTHLLTLRLDENKFSGVIPELNLPGLQDFNVSCNRFSGKIPKTLSGFSGSSFGQNPFLCGAPLEKCGDEPNKPGSDGAIASPLVPATVVSSSPSTMPTTTATPTTKNHEKRGDVLVLGIVCLLLYCYFWKNYSSKSKDKKGLKLFESEKIVYSSSPYPTQGGFERGRMVFFEGGFGTAYKAVLDDGNVVAVKRLKDAQIAGKREFEQHMEILGRIRHPNVVSLRAYYFARDEKLLVYDYMPNATLFWLLHGNRGPGRTPLDWTTRLKIAAGAAHGIAFIHNSCKSLKLTHGNIKSTNILLDKQGDARVSDFGLSVFNGSSPSGAGSRSNGYRAPEVLDGRKQSQKSDVYSFGVLLLEMLTGKCPSAVESGGSGYNGGVIDLPRWVQSVVREEWTAEVFDLELMRYKDIEEEMVGLLQIAMSCTAASPDQRPRMSHVVKMIEELRGVEVSPCHDTVDSVSDSPSLSEDACAGTTSQ*