AlfalfaGEDB | Msa0915520

M. sativa cultivar zhongmu-4 / Msa0915520

KEGG: K10403
GO: GO:0000070 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006890 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007059 GO:0007062 GO:0007080 GO:0008104 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016043 GO:0016192 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019882 GO:0019884 GO:0019886 GO:0022402 GO:0030705 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0042623 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046907 GO:0048002 GO:0048193 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0070013 GO:0071840 GO:0072686 GO:0097159 GO:0098687 GO:0098813 GO:0140014 GO:1901363 GO:1903047 GO:1990939
PFAM: PF00225.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0915520	XP_013452365.1	97.066	852	24	1	4	854	49	900	0.0	1692

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0915520	sp\|F4KEC6\|KN10C_ARATH	52.788	269	119	5	6	273	55	316	6.26e-80	275
Msa0915520	sp\|F4KEC6\|KN10C_ARATH	57.843	102	40	2	752	853	603	701	3.85e-28	124

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002580	Msa0915520	0.806749	7.071793e-50	-8.615850e-47
Msa0005020	Msa0915520	0.810272	1.251541e-50	-8.615850e-47
Msa0005890	Msa0915520	0.836744	7.818393e-57	-8.615850e-47
Msa0016040	Msa0915520	0.868049	8.714464e-66	-8.615850e-47
Msa0019930	Msa0915520	0.818068	2.377548e-52	-8.615850e-47
Msa0020540	Msa0915520	0.806526	7.880786e-50	-8.615850e-47
Msa0025580	Msa0915520	0.834109	3.623979e-56	-8.615850e-47
Msa0027280	Msa0915520	0.872215	3.772464e-67	-8.615850e-47
Msa0035140	Msa0915520	0.853282	2.649285e-61	-8.615850e-47
Msa0035340	Msa0915520	0.824057	9.920850e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0915520	MtrunA17_Chr6g0470541	97.066	852	24	1	4	854	49	900	0.0	1692
Msa0915520	MtrunA17_Chr7g0238581	39.812	319	182	6	6	315	79	396	1.14e-60	217
Msa0915520	MtrunA17_Chr7g0238581	40.496	121	65	3	739	854	511	629	5.99e-18	88.6
Msa0915520	MtrunA17_Chr1g0182151	28.716	296	180	9	6	272	101	394	5.16e-29	125
Msa0915520	MtrunA17_Chr2g0307481	30.000	260	172	6	6	257	119	376	5.63e-28	120
Msa0915520	MtrunA17_Chr3g0132921	30.634	284	162	9	6	257	48	328	2.52e-27	117
Msa0915520	MtrunA17_Chr4g0035661	31.769	277	176	10	6	272	130	403	2.04e-26	116
Msa0915520	MtrunA17_Chr5g0407791	28.986	276	175	5	2	256	914	1189	8.22e-26	114
Msa0915520	MtrunA17_Chr3g0145071	31.408	277	177	10	6	272	140	413	1.16e-25	114
Msa0915520	MtrunA17_Chr2g0331761	31.095	283	165	12	6	271	235	504	6.24e-25	111

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0915520	AT5G23910.2	52.788	269	119	5	6	273	59	320	5.64e-81	275
Msa0915520	AT5G23910.2	57.843	102	40	2	752	853	607	705	3.90e-29	124
Msa0915520	AT5G23910.1	52.788	269	119	5	6	273	55	316	6.36e-81	275
Msa0915520	AT5G23910.1	57.843	102	40	2	752	853	603	701	3.91e-29	124
Msa0915520	AT5G02370.1	47.037	270	134	5	6	272	74	337	6.40e-65	228
Msa0915520	AT1G18550.1	30.566	265	174	6	1	257	199	461	2.05e-27	119
Msa0915520	AT2G37420.1	29.964	277	168	8	6	257	95	370	5.35e-25	112
Msa0915520	AT2G37420.2	29.964	277	168	8	6	257	95	370	5.35e-25	112
Msa0915520	AT5G65930.3	28.938	273	173	5	6	257	923	1195	8.38e-25	112
Msa0915520	AT5G65930.2	28.938	273	173	5	6	257	917	1189	8.50e-25	112

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Find 153 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGCTCTTTGGAGAGTC+CGG	0.395682	6_2:-31936798	Msa0915520:intron
AACAGTACTGTTATTGCTCA+TGG	0.404603	6_2:-31936972	Msa0915520:CDS
AACTAGACAGATAGCGACCT+TGG	0.500907	6_2:-31935785	Msa0915520:CDS
AACTCAGAAATGGATTTAAC+AGG	0.413773	6_2:+31936496	None:intergenic
AACTTTGCTAGTTCCCCTCA+AGG	0.619759	6_2:+31936038	None:intergenic
AAGGGCTCTTTGGAGAGTCC+GGG	0.524720	6_2:-31936797	Msa0915520:CDS
AAGTTCAGCAAGACAGACAG+TGG	0.682432	6_2:-31935407	Msa0915520:CDS
AAGTTCAGCAATACATACAG+TGG	0.693549	6_2:-31935359	Msa0915520:CDS
AAGTTCAGTAAGACAGAACG+TGG	0.690570	6_2:-31935551	Msa0915520:CDS
AATAAATACCAAAAGTCCTT+GGG	0.481537	6_2:-31933511	Msa0915520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGAAAAACTCACTTAATTC+AGG	-	chr6_2:31932083-31932102	Msa0915520:three_prime_UTR	30.0%
GATTTAATAAATCGACAGCA+TGG	+	chr6_2:31932342-31932361	None:intergenic	30.0%
TAAATACTTCAACAAAAGCC+AGG	+	chr6_2:31932762-31932781	None:intergenic	30.0%
ATTGTTCTTTGTGGAATCTA+AGG	+	chr6_2:31933351-31933370	None:intergenic	30.0%
GCAACAAATTCTGCAATAAA+AGG	-	chr6_2:31933869-31933888	Msa0915520:CDS	30.0%
CAAATTCTGCAATAAAAGGA+AGG	-	chr6_2:31933873-31933892	Msa0915520:CDS	30.0%
TTTATACTGGTATCTGGTTA+TGG	-	chr6_2:31933945-31933964	Msa0915520:intron	30.0%
ATATGTATGTGTGTACTGTT+TGG	-	chr6_2:31934223-31934242	Msa0915520:intron	30.0%
TGTCTAAGTTTAGTCATGTT+AGG	+	chr6_2:31934431-31934450	None:intergenic	30.0%
AACATGACTAAACTTAGACA+TGG	-	chr6_2:31934431-31934450	Msa0915520:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	31931937	31937105	31931937	ID=Msa0915520;Name=Msa0915520
chr6_2	mRNA	31931937	31937105	31931937	ID=Msa0915520-mRNA-1;Parent=Msa0915520;Name=Msa0915520-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0\|0.94\|0.84\|1\|1\|1\|19\|262\|854
chr6_2	exon	31931937	31932243	31931937	ID=Msa0915520-mRNA-1:exon:5771;Parent=Msa0915520-mRNA-1
chr6_2	exon	31932360	31932398	31932360	ID=Msa0915520-mRNA-1:exon:5770;Parent=Msa0915520-mRNA-1
chr6_2	exon	31932532	31932600	31932532	ID=Msa0915520-mRNA-1:exon:5769;Parent=Msa0915520-mRNA-1
chr6_2	exon	31932686	31932707	31932686	ID=Msa0915520-mRNA-1:exon:5768;Parent=Msa0915520-mRNA-1
chr6_2	exon	31932965	31933011	31932965	ID=Msa0915520-mRNA-1:exon:5767;Parent=Msa0915520-mRNA-1
chr6_2	exon	31933094	31933201	31933094	ID=Msa0915520-mRNA-1:exon:5766;Parent=Msa0915520-mRNA-1
chr6_2	exon	31933286	31933393	31933286	ID=Msa0915520-mRNA-1:exon:5765;Parent=Msa0915520-mRNA-1
chr6_2	exon	31933479	31933744	31933479	ID=Msa0915520-mRNA-1:exon:5764;Parent=Msa0915520-mRNA-1
chr6_2	exon	31933828	31933897	31933828	ID=Msa0915520-mRNA-1:exon:5763;Parent=Msa0915520-mRNA-1
chr6_2	exon	31934042	31934098	31934042	ID=Msa0915520-mRNA-1:exon:5762;Parent=Msa0915520-mRNA-1
chr6_2	exon	31934307	31934363	31934307	ID=Msa0915520-mRNA-1:exon:5761;Parent=Msa0915520-mRNA-1
chr6_2	exon	31934454	31934498	31934454	ID=Msa0915520-mRNA-1:exon:5760;Parent=Msa0915520-mRNA-1
chr6_2	exon	31934634	31934690	31934634	ID=Msa0915520-mRNA-1:exon:5759;Parent=Msa0915520-mRNA-1
chr6_2	exon	31935001	31935046	31935001	ID=Msa0915520-mRNA-1:exon:5758;Parent=Msa0915520-mRNA-1
chr6_2	exon	31935148	31935914	31935148	ID=Msa0915520-mRNA-1:exon:5757;Parent=Msa0915520-mRNA-1
chr6_2	exon	31936020	31936225	31936020	ID=Msa0915520-mRNA-1:exon:5756;Parent=Msa0915520-mRNA-1
chr6_2	exon	31936335	31936521	31936335	ID=Msa0915520-mRNA-1:exon:5755;Parent=Msa0915520-mRNA-1
chr6_2	exon	31936616	31936816	31936616	ID=Msa0915520-mRNA-1:exon:5754;Parent=Msa0915520-mRNA-1
chr6_2	exon	31936938	31937105	31936938	ID=Msa0915520-mRNA-1:exon:5753;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31936938	31937105	31936938	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31936616	31936816	31936616	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31936335	31936521	31936335	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31936020	31936225	31936020	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31935148	31935914	31935148	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31935001	31935046	31935001	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31934634	31934690	31934634	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31934454	31934498	31934454	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31934307	31934363	31934307	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31934042	31934098	31934042	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31933828	31933897	31933828	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31933479	31933744	31933479	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31933286	31933393	31933286	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31933094	31933201	31933094	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31932965	31933011	31932965	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31932686	31932707	31932686	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31932532	31932600	31932532	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31932360	31932398	31932360	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	CDS	31932199	31932243	31932199	ID=Msa0915520-mRNA-1:cds;Parent=Msa0915520-mRNA-1
chr6_2	three_prime_UTR	31931937	31932198	31931937	ID=Msa0915520-mRNA-1:three_prime_utr;Parent=Msa0915520-mRNA-1

Gene Sequence

>Msa0915520

TTGTGGTGCAGTCGGTATTCAGTGGACTACTGCTATAACGAACATGATGATAATGAAATGATCTATTCAAGGGAAGTGAAGCCTCTTGTTTCGGCAGCTTTTGAAGGTTTTAACAGTACTGTTATTGCTCATGGAGCTAGAGGCAGTGGAAAAACTCACTTAATTCAGGGCTCTTTGGAGAGTCCGGGTTTGGCAGCGCTGGCTACGTCTGAGTTTCTCTCCATGGCTGAGAAAAATGGAAAATCTATAGCAGTTTCTTTCTACGAGGTTGATCATCAGGACCATGCTGTCGATTTATTAAATCCAGAACAACCACATATTTTGGTGCTTGAAGATCACGGAAGAATCCAGTTTAAAGGACTCAGTCAGGCTCCTGTTAAATCCATTTCTGAGTTCCAGAATTTATACATCGCTGCATGTTCTGCAAACAAGGCTGCACCCAAAAAAGGCATTGAGCGTGCTCATAGAAGCCACATGGGTTTGATTGTGCATGTCTTTTCCCAAAATGAAAGCATGAATGGTCTTGTGAGCAAAATGAATTTTGTGGATATGGCAGGTTACGAAGATGCTAGAAGGAAAAGTAGTGATGCCTCTGGTCATGTTGAGAATAACAAACTTAACAAGTCAATTTACGCCTTACTGAATGTTTGTCAGGCGTTGAGTACAAATGAAAGCCGTGTTCCCTTCCGGGAAAGTAAACTTACACGCATATTGCAGGACTCCTTGAGGGGAACTAGCAAAGTTTTGTTGGTTTCATGCTTGAATCCATCGTTTTGCCAAGACACTGTTTACATGGTAAGCTTAGCATCACGGTCATGTCAATCGATTCATCAAACCCCCTTAGACTCCATGAAGAAAACTGCAAGTTCAACTAGACAGATAGCGACCTTGGATTCCACAAAGAAAAGTGCAAGTTCTGCAAGAGAGATAGCAACCTTAGATTCCACAAAGAAAAATGCAAGTTCAGCAAGAAAAATAGTGACCTTAGATTCCACAAAGAACAATGCAAGCTCAGTAAGACAGAACGTAGCCACAGATTCGTCAAACAAAAAATCAAGCTCAGTAAGACAGACAGTAACCTTAGATTCCACAAAGAAAAGTGCAAGTTCAGTAAGACAGAACGTGGCCTCAGATTCTTCAAAGAAAATATCAAGTTCAGTAAGACAGACAGTAACCTTTGCTTCCACAAAGAAAAGTGCAAGTTCAGCAATACAGACAGTGACCTCAGATTCCACAAAGAAAACTGCAAGTTCAGCAAGACAGACAGTGGCCTCAGATTCAACAAAAAAAACTGCAAGTTCAGCAATACATACAGTGGCCTCAGATTCCACCAAGAAAAGTGCAAGTTCAGCTATACAGATAGTAGCTGATTCAACAAAGAAAAGCGGAAATTCAGCAATACTGACAGTGACTTATCGCAAAAAAAAGATACCCCAAAGTGTATCAGCGTCAACAACGAAATTACCAGGCTCCATATCTCATATAAAGGAAAATGGTGTTATTGCAACAAATTCTGCAATAAAAGGAAGGAAATTGTTTGATGAAGCCAGCCACTCTGCTGTTAAAGCTGAGAAGGACAATTCAATATCAAAAGATGGAAATGACGCCCAAGTAAACTCTAGAGTAAATAAGGAAATTCCCATTGCTGATATTACTAGAGCATCGGAGTCTTTATTGGACAATTCGTTATCAGAAGATGGAAATCACGATGAACTAAACTCTAGAGTTGAAAAGGATAACTCATCTTTGAATGCTAGCAGTGAAGTGGAATTTGATCCCACAGTGGAAAAGGTTATTTCGTTCGAGGATAAACACCCATATGTCATTAATTACTCCAAAGATTTGTCGATAGCAGATGAAGGTCATAACATGAACAAGGAGAACAACAACTTAATGGAAAATGAAGATTTTTCACCACCCATTAGTTCTCAATTGCGTGAACTTCAATCTCTCGTTTCGTCAACTCCATTGTGTATGCAGTTACCAGAGAACGGATGCATTTCACATGACGATGATCAGATTTCTGCTAAAATTGCAGAACCAACAACTCTTGATACCAAAAGACGAGATGTAATAAATACCAAAAGTCCTTGGGAAACATTCAACAAGCACGGTTCTGAAATGGAGAATTCAACCGAAATTACAGAACCAAGAACTCCCGATGCTGGAAGAGGAGATGTAATGAATACCAAAAGTCCTTGGGAAACATTCAACATGAATGGTTCTGGAATGAAGAATTCCACTGAAATTACAGAACCAAGAACTCCTGATACTGGAAGACGAGATGTAATGAATACCAAAAGTCCTTGGGAAACATTCAATTTGCATGGTTCTGGAATGAAGAATTCTCTTGTTAAGGACTACCTTAGAATTTTAAACACAGCTGAAAAGGATGAATTAAAGAAGTTAAAGGGAATTGGAGAGAAGAGAGCAACCTATATACTTGAGCTTCGTGAAGAATCTCCAGAACCTTTCAAGAGTCTTGATGATTTGAAGGACATTGGACTTTCTGAAAAACAGATTAAAGGGATGATGAAGAAGGAAGTTGGAGAGCTTTTCAATTAG

Protein sequence

>Msa0915520

LWCSRYSVDYCYNEHDDNEMIYSREVKPLVSAAFEGFNSTVIAHGARGSGKTHLIQGSLESPGLAALATSEFLSMAEKNGKSIAVSFYEVDHQDHAVDLLNPEQPHILVLEDHGRIQFKGLSQAPVKSISEFQNLYIAACSANKAAPKKGIERAHRSHMGLIVHVFSQNESMNGLVSKMNFVDMAGYEDARRKSSDASGHVENNKLNKSIYALLNVCQALSTNESRVPFRESKLTRILQDSLRGTSKVLLVSCLNPSFCQDTVYMVSLASRSCQSIHQTPLDSMKKTASSTRQIATLDSTKKSASSAREIATLDSTKKNASSARKIVTLDSTKNNASSVRQNVATDSSNKKSSSVRQTVTLDSTKKSASSVRQNVASDSSKKISSSVRQTVTFASTKKSASSAIQTVTSDSTKKTASSARQTVASDSTKKTASSAIHTVASDSTKKSASSAIQIVADSTKKSGNSAILTVTYRKKKIPQSVSASTTKLPGSISHIKENGVIATNSAIKGRKLFDEASHSAVKAEKDNSISKDGNDAQVNSRVNKEIPIADITRASESLLDNSLSEDGNHDELNSRVEKDNSSLNASSEVEFDPTVEKVISFEDKHPYVINYSKDLSIADEGHNMNKENNNLMENEDFSPPISSQLRELQSLVSSTPLCMQLPENGCISHDDDQISAKIAEPTTLDTKRRDVINTKSPWETFNKHGSEMENSTEITEPRTPDAGRGDVMNTKSPWETFNMNGSGMKNSTEITEPRTPDTGRRDVMNTKSPWETFNLHGSGMKNSLVKDYLRILNTAEKDELKKLKGIGEKRATYILELREESPEPFKSLDDLKDIGLSEKQIKGMMKKEVGELFN*