AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0120300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0120300 MtrunA17_Chr5g0411971 67.347 49 16 0 6 54 250 298 3.11e-12 60.1
Msa0120300 MtrunA17_Chr5g0421591 65.306 49 17 0 6 54 225 273 1.26e-11 58.5
Msa0120300 MtrunA17_Chr5g0421521 65.306 49 17 0 6 54 225 273 1.50e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGGTGTATCTTGATGTTAT+TGG 0.292074 1_3:+44968534 Msa0120300:CDS
TGATGTTATTGGTTTATCTT+TGG 0.293902 1_3:+44968545 Msa0120300:CDS
GTAAGATAAAGGATCTAAAT+AGG 0.299573 1_3:+44967507 Msa0120300:CDS
AGTTGAAGAGAGTAAGATAA+AGG 0.391395 1_3:+44967496 Msa0120300:CDS
ATCTTGTTATTTAAAGTGTA+AGG 0.392769 1_3:+44968626 Msa0120300:three_prime_UTR
TCACTTGATCAAGCTCCATC+TGG 0.433643 1_3:-44967607 Msa0120290:intron
TTGGTTGACATGGCTCTGAC+TGG 0.443153 1_3:+44968564 Msa0120300:CDS
TGGTTTATCTTTGGTTGACA+TGG 0.450345 1_3:+44968554 Msa0120300:CDS
AATAACAAGATTAAAACTAA+AGG 0.474612 1_3:-44968614 Msa0120290:intron
AAATAGGTTGTTCGAGCTCA+AGG 0.489118 1_3:+44967523 Msa0120300:CDS
TTGAAATGAGTAGGAAAATG+TGG 0.496728 1_3:+44967579 Msa0120300:CDS
CGTGCAAAACATACTGGCAG+TGG 0.521936 1_3:-44967642 Msa0120290:intron
GGTCATTAATTGAAATGAGT+AGG 0.571372 1_3:+44967570 Msa0120300:CDS
AAGAATGCTATGAAGTGTGT+AGG 0.581200 1_3:+44967549 Msa0120300:CDS
GAAAATGTGGAAATTCCAGA+TGG 0.590028 1_3:+44967592 Msa0120300:CDS
GTGCAAAACATACTGGCAGT+GGG 0.648147 1_3:-44967641 Msa0120290:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATCCAAAATAATATTA+AGG - chr1_3:44968464-44968483 Msa0120290:intron 10.0%
!! AATTATCCAAAATAATATTA+AGG - chr1_3:44968464-44968483 Msa0120290:intron 10.0%
!! AATTCTTGAATTAAATAAGA+TGG + chr1_3:44968010-44968029 Msa0120300:intron 15.0%
!! AATAGTATGAATTGTATTAA+AGG + chr1_3:44968127-44968146 Msa0120300:intron 15.0%
!! ATAGTATGAATTGTATTAAA+GGG + chr1_3:44968128-44968147 Msa0120300:intron 15.0%
!! TAATAGTTGAAAGAAATATT+AGG + chr1_3:44968346-44968365 Msa0120300:intron 15.0%
!! AATAACAAGATTAAAACTAA+AGG - chr1_3:44968617-44968636 Msa0120290:intron 15.0%
!! AATTCTTGAATTAAATAAGA+TGG + chr1_3:44968010-44968029 Msa0120300:intron 15.0%
!! AATAGTATGAATTGTATTAA+AGG + chr1_3:44968127-44968146 Msa0120300:intron 15.0%
!! ATAGTATGAATTGTATTAAA+GGG + chr1_3:44968128-44968147 Msa0120300:intron 15.0%
!! TAATAGTTGAAAGAAATATT+AGG + chr1_3:44968346-44968365 Msa0120300:intron 15.0%
!! AATAACAAGATTAAAACTAA+AGG - chr1_3:44968617-44968636 Msa0120290:intron 15.0%
!!! TTTGTGCCTTAATATTATTT+TGG + chr1_3:44968455-44968474 Msa0120300:intron 20.0%
!!! ATCTTGTTATTTAAAGTGTA+AGG + chr1_3:44968626-44968645 Msa0120300:three_prime_UTR 20.0%
!!! TTTGTGCCTTAATATTATTT+TGG + chr1_3:44968455-44968474 Msa0120300:intron 20.0%
!!! ATCTTGTTATTTAAAGTGTA+AGG + chr1_3:44968626-44968645 Msa0120300:three_prime_UTR 20.0%
! GTAAGATAAAGGATCTAAAT+AGG + chr1_3:44967507-44967526 Msa0120300:CDS 25.0%
!!! ATTTACTTTTCGTTGTTAAC+TGG - chr1_3:44967824-44967843 Msa0120290:intron 25.0%
!!! TATGGGACTTTTTTTTTAGA+GGG - chr1_3:44967963-44967982 Msa0120290:intron 25.0%
!!! ATATGGGACTTTTTTTTTAG+AGG - chr1_3:44967964-44967983 Msa0120290:intron 25.0%
! TCAGTCTTAAGTAATAAAGA+TGG - chr1_3:44968079-44968098 Msa0120290:intron 25.0%
!!! TTTTTTTTTGCAGTGTTTCA+AGG + chr1_3:44968504-44968523 Msa0120300:intron 25.0%
!!! TGATGTTATTGGTTTATCTT+TGG + chr1_3:44968545-44968564 Msa0120300:CDS 25.0%
! GTAAGATAAAGGATCTAAAT+AGG + chr1_3:44967507-44967526 Msa0120300:CDS 25.0%
!!! ATTTACTTTTCGTTGTTAAC+TGG - chr1_3:44967824-44967843 Msa0120290:intron 25.0%
!!! TATGGGACTTTTTTTTTAGA+GGG - chr1_3:44967963-44967982 Msa0120290:intron 25.0%
!!! ATATGGGACTTTTTTTTTAG+AGG - chr1_3:44967964-44967983 Msa0120290:intron 25.0%
! TCAGTCTTAAGTAATAAAGA+TGG - chr1_3:44968079-44968098 Msa0120290:intron 25.0%
!!! TTTTTTTTTGCAGTGTTTCA+AGG + chr1_3:44968504-44968523 Msa0120300:intron 25.0%
!!! TGATGTTATTGGTTTATCTT+TGG + chr1_3:44968545-44968564 Msa0120300:CDS 25.0%
AGTTGAAGAGAGTAAGATAA+AGG + chr1_3:44967496-44967515 Msa0120300:CDS 30.0%
GGTCATTAATTGAAATGAGT+AGG + chr1_3:44967570-44967589 Msa0120300:CDS 30.0%
TTGAAATGAGTAGGAAAATG+TGG + chr1_3:44967579-44967598 Msa0120300:CDS 30.0%
ATATTCCAACAAGAGAAGTT+TGG + chr1_3:44967665-44967684 Msa0120300:intron 30.0%
ATTTACTGTCATGTTTACGT+AGG + chr1_3:44967730-44967749 Msa0120300:intron 30.0%
CCATTTCTGACAGTTTATTA+CGG - chr1_3:44967788-44967807 Msa0120290:intron 30.0%
CGTAATAAACTGTCAGAAAT+GGG + chr1_3:44967786-44967805 Msa0120300:intron 30.0%
!!! ATGGGACTTTTTTTTTAGAG+GGG - chr1_3:44967962-44967981 Msa0120290:intron 30.0%
TATTGACATATGTGCTTCAA+GGG - chr1_3:44967989-44968008 Msa0120290:intron 30.0%
ATATTGACATATGTGCTTCA+AGG - chr1_3:44967990-44968009 Msa0120290:intron 30.0%
GTCTTAAGTAATAAAGATGG+AGG - chr1_3:44968076-44968095 Msa0120290:intron 30.0%
! GGCTAAAAATTATTTTCCGT+AGG + chr1_3:44968149-44968168 Msa0120300:intron 30.0%
! AAAGCTCTATGATGATTTTC+AGG + chr1_3:44968224-44968243 Msa0120300:intron 30.0%
AGTTGAAGAGAGTAAGATAA+AGG + chr1_3:44967496-44967515 Msa0120300:CDS 30.0%
GGTCATTAATTGAAATGAGT+AGG + chr1_3:44967570-44967589 Msa0120300:CDS 30.0%
TTGAAATGAGTAGGAAAATG+TGG + chr1_3:44967579-44967598 Msa0120300:CDS 30.0%
ATATTCCAACAAGAGAAGTT+TGG + chr1_3:44967665-44967684 Msa0120300:intron 30.0%
ATTTACTGTCATGTTTACGT+AGG + chr1_3:44967730-44967749 Msa0120300:intron 30.0%
CCATTTCTGACAGTTTATTA+CGG - chr1_3:44967788-44967807 Msa0120290:intron 30.0%
CGTAATAAACTGTCAGAAAT+GGG + chr1_3:44967786-44967805 Msa0120300:intron 30.0%
!!! ATGGGACTTTTTTTTTAGAG+GGG - chr1_3:44967962-44967981 Msa0120290:intron 30.0%
TATTGACATATGTGCTTCAA+GGG - chr1_3:44967989-44968008 Msa0120290:intron 30.0%
ATATTGACATATGTGCTTCA+AGG - chr1_3:44967990-44968009 Msa0120290:intron 30.0%
GTCTTAAGTAATAAAGATGG+AGG - chr1_3:44968076-44968095 Msa0120290:intron 30.0%
! GGCTAAAAATTATTTTCCGT+AGG + chr1_3:44968149-44968168 Msa0120300:intron 30.0%
! AAAGCTCTATGATGATTTTC+AGG + chr1_3:44968224-44968243 Msa0120300:intron 30.0%
AAGAATGCTATGAAGTGTGT+AGG + chr1_3:44967549-44967568 Msa0120300:CDS 35.0%
GAAAATGTGGAAATTCCAGA+TGG + chr1_3:44967592-44967611 Msa0120300:CDS 35.0%
GAATATCGTGCAAAACATAC+TGG - chr1_3:44967651-44967670 Msa0120290:intron 35.0%
! GTTTTCCAAACTTCTCTTGT+TGG - chr1_3:44967673-44967692 Msa0120290:intron 35.0%
! ATCTGATGATGCATAGCTTA+TGG + chr1_3:44967762-44967781 Msa0120300:intron 35.0%
CCGTAATAAACTGTCAGAAA+TGG + chr1_3:44967785-44967804 Msa0120300:intron 35.0%
!!! TGGGACTTTTTTTTTAGAGG+GGG - chr1_3:44967961-44967980 Msa0120290:intron 35.0%
ATGTGCTTCAAGGGAAATAT+GGG - chr1_3:44967980-44967999 Msa0120290:intron 35.0%
TATGTGCTTCAAGGGAAATA+TGG - chr1_3:44967981-44968000 Msa0120290:intron 35.0%
TGGTTTATCTTTGGTTGACA+TGG + chr1_3:44968554-44968573 Msa0120300:CDS 35.0%
AAGAATGCTATGAAGTGTGT+AGG + chr1_3:44967549-44967568 Msa0120300:CDS 35.0%
GAAAATGTGGAAATTCCAGA+TGG + chr1_3:44967592-44967611 Msa0120300:CDS 35.0%
GAATATCGTGCAAAACATAC+TGG - chr1_3:44967651-44967670 Msa0120290:intron 35.0%
! GTTTTCCAAACTTCTCTTGT+TGG - chr1_3:44967673-44967692 Msa0120290:intron 35.0%
! ATCTGATGATGCATAGCTTA+TGG + chr1_3:44967762-44967781 Msa0120300:intron 35.0%
CCGTAATAAACTGTCAGAAA+TGG + chr1_3:44967785-44967804 Msa0120300:intron 35.0%
!!! TGGGACTTTTTTTTTAGAGG+GGG - chr1_3:44967961-44967980 Msa0120290:intron 35.0%
ATGTGCTTCAAGGGAAATAT+GGG - chr1_3:44967980-44967999 Msa0120290:intron 35.0%
TATGTGCTTCAAGGGAAATA+TGG - chr1_3:44967981-44968000 Msa0120290:intron 35.0%
TGGTTTATCTTTGGTTGACA+TGG + chr1_3:44968554-44968573 Msa0120300:CDS 35.0%
AAATAGGTTGTTCGAGCTCA+AGG + chr1_3:44967523-44967542 Msa0120300:CDS 40.0%
TAAGATGGTTTATGCAGTGC+AGG + chr1_3:44968025-44968044 Msa0120300:intron 40.0%
ATCTTCACACTGCTTACCTA+CGG - chr1_3:44968168-44968187 Msa0120290:intron 40.0%
TTTATGGCCCTAAGTGTCAT+GGG - chr1_3:44968397-44968416 Msa0120290:intron 40.0%
GTTTCCACTGTGATCGTTTA+TGG - chr1_3:44968413-44968432 Msa0120290:intron 40.0%
! TGCAGTGTTTCAAGGTTTTC+TGG + chr1_3:44968512-44968531 Msa0120300:intron 40.0%
! GCAGTGTTTCAAGGTTTTCT+GGG + chr1_3:44968513-44968532 Msa0120300:intron 40.0%
! CAGTGTTTCAAGGTTTTCTG+GGG + chr1_3:44968514-44968533 Msa0120300:intron 40.0%
! AGTGTTTCAAGGTTTTCTGG+GGG + chr1_3:44968515-44968534 Msa0120300:intron 40.0%
GGGGTGTATCTTGATGTTAT+TGG + chr1_3:44968534-44968553 Msa0120300:CDS 40.0%
AAATAGGTTGTTCGAGCTCA+AGG + chr1_3:44967523-44967542 Msa0120300:CDS 40.0%
TAAGATGGTTTATGCAGTGC+AGG + chr1_3:44968025-44968044 Msa0120300:intron 40.0%
ATCTTCACACTGCTTACCTA+CGG - chr1_3:44968168-44968187 Msa0120290:intron 40.0%
TTTATGGCCCTAAGTGTCAT+GGG - chr1_3:44968397-44968416 Msa0120290:intron 40.0%
GTTTCCACTGTGATCGTTTA+TGG - chr1_3:44968413-44968432 Msa0120290:intron 40.0%
! TGCAGTGTTTCAAGGTTTTC+TGG + chr1_3:44968512-44968531 Msa0120300:intron 40.0%
! GCAGTGTTTCAAGGTTTTCT+GGG + chr1_3:44968513-44968532 Msa0120300:intron 40.0%
! CAGTGTTTCAAGGTTTTCTG+GGG + chr1_3:44968514-44968533 Msa0120300:intron 40.0%
! AGTGTTTCAAGGTTTTCTGG+GGG + chr1_3:44968515-44968534 Msa0120300:intron 40.0%
GGGGTGTATCTTGATGTTAT+TGG + chr1_3:44968534-44968553 Msa0120300:CDS 40.0%
TCACTTGATCAAGCTCCATC+TGG - chr1_3:44967610-44967629 Msa0120290:intron 45.0%
GTGCAAAACATACTGGCAGT+GGG - chr1_3:44967644-44967663 Msa0120290:intron 45.0%
!! GATGATTTTCAGGACGCTGA+AGG + chr1_3:44968234-44968253 Msa0120300:intron 45.0%
TACTTCAGCCCATGACACTT+AGG + chr1_3:44968386-44968405 Msa0120300:intron 45.0%
ACTTCAGCCCATGACACTTA+GGG + chr1_3:44968387-44968406 Msa0120300:intron 45.0%
GTTTATGGCCCTAAGTGTCA+TGG - chr1_3:44968398-44968417 Msa0120290:intron 45.0%
TCACTTGATCAAGCTCCATC+TGG - chr1_3:44967610-44967629 Msa0120290:intron 45.0%
GTGCAAAACATACTGGCAGT+GGG - chr1_3:44967644-44967663 Msa0120290:intron 45.0%
!! GATGATTTTCAGGACGCTGA+AGG + chr1_3:44968234-44968253 Msa0120300:intron 45.0%
TACTTCAGCCCATGACACTT+AGG + chr1_3:44968386-44968405 Msa0120300:intron 45.0%
ACTTCAGCCCATGACACTTA+GGG + chr1_3:44968387-44968406 Msa0120300:intron 45.0%
GTTTATGGCCCTAAGTGTCA+TGG - chr1_3:44968398-44968417 Msa0120290:intron 45.0%
CGTGCAAAACATACTGGCAG+TGG - chr1_3:44967645-44967664 Msa0120290:intron 50.0%
AGGGCCATAAACGATCACAG+TGG + chr1_3:44968406-44968425 Msa0120300:intron 50.0%
!! TTGGTTGACATGGCTCTGAC+TGG + chr1_3:44968564-44968583 Msa0120300:CDS 50.0%
CGTGCAAAACATACTGGCAG+TGG - chr1_3:44967645-44967664 Msa0120290:intron 50.0%
AGGGCCATAAACGATCACAG+TGG + chr1_3:44968406-44968425 Msa0120300:intron 50.0%
!! TTGGTTGACATGGCTCTGAC+TGG + chr1_3:44968564-44968583 Msa0120300:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 44967488 44968652 44967488 ID=Msa0120300;Name=Msa0120300
chr1_3 mRNA 44967488 44968652 44967488 ID=Msa0120300-mRNA-1;Parent=Msa0120300;Name=Msa0120300-mRNA-1;_AED=0.72;_eAED=0.72;_QI=0|0|0.5|0.5|1|1|2|56|80
chr1_3 exon 44967488 44967650 44967488 ID=Msa0120300-mRNA-1:exon:13462;Parent=Msa0120300-mRNA-1
chr1_3 exon 44968517 44968652 44968517 ID=Msa0120300-mRNA-1:exon:13463;Parent=Msa0120300-mRNA-1
chr1_3 CDS 44967488 44967650 44967488 ID=Msa0120300-mRNA-1:cds;Parent=Msa0120300-mRNA-1
chr1_3 CDS 44968517 44968596 44968517 ID=Msa0120300-mRNA-1:cds;Parent=Msa0120300-mRNA-1
chr1_3 three_prime_UTR 44968597 44968652 44968597 ID=Msa0120300-mRNA-1:three_prime_utr;Parent=Msa0120300-mRNA-1
Gene Sequence

>Msa0120300

ATGGAGTTAGTTGAAGAGAGTAAGATAAAGGATCTAAATAGGTTGTTCGAGCTCAAGGAAAAAGAATGCTATGAAGTGTGTAGGTCATTAATTGAAATGAGTAGGAAAATGTGGAAATTCCAGATGGAGCTTGATCAAGTGACATTTAAATCACCCACTGCCATGTTTCAAGGTTTTCTGGGGGTGTATCTTGATGTTATTGGTTTATCTTTGGTTGACATGGCTCTGACTGGTTGTTTGTAG

Protein sequence

>Msa0120300

MELVEESKIKDLNRLFELKEKECYEVCRSLIEMSRKMWKFQMELDQVTFKSPTAMFQGFLGVYLDVIGLSLVDMALTGCL*