AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0141930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0141930 MtrunA17_Chr4g0064451 98.844 173 2 0 1 173 988 1160 2.89e-119 363
Msa0141930 MtrunA17_Chr4g0064641 95.954 173 7 0 1 173 928 1100 4.38e-116 353
Msa0141930 MtrunA17_Chr1g0212891 94.220 173 10 0 1 173 1042 1214 2.81e-115 353
Msa0141930 MtrunA17_Chr6g0453881 92.903 155 11 0 15 169 22 176 1.69e-107 304
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0141930 AT3G55220.1 88.439 173 20 0 1 173 1042 1214 7.43e-108 333
Msa0141930 AT3G55200.1 88.439 173 20 0 1 173 1042 1214 7.43e-108 333
Msa0141930 AT3G55200.3 88.439 173 20 0 1 173 1042 1214 7.43e-108 333
Msa0141930 AT3G55200.2 88.439 173 20 0 1 173 1042 1214 7.43e-108 333

Find 99 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATTTCTTCAACCTTAT+TGG 0.095011 1_4:-698433 None:intergenic
TCCAGCAGTTAAACTCCTTT+TGG 0.246980 1_4:-697606 None:intergenic
ACAATTTCTTCAACCTTATT+GGG 0.293512 1_4:-698432 None:intergenic
AGTTTAGTATATACCTTAAC+AGG 0.303468 1_4:-698681 None:intergenic
TGTGCTCAGGCTATATGATT+TGG 0.319803 1_4:+697754 Msa0141930:five_prime_UTR
ATATACCTTAACAGGAAAAT+AGG 0.320570 1_4:-698673 None:intergenic
CTGGATTCATTCATATATAT+AGG 0.323354 1_4:+697624 Msa0141930:five_prime_UTR
TGATCACCGTTGGCATCTCC+TGG 0.350090 1_4:+700295 Msa0141930:three_prime_UTR
GGTGGAGAGTGCGTTGTATA+TGG 0.364463 1_4:+698513 Msa0141930:CDS
CGTTGTATATGGAACAGTTA+TGG 0.369151 1_4:+698524 Msa0141930:CDS
TTTATAAGAGACCTGCAAAA+TGG 0.382364 1_4:+700193 Msa0141930:three_prime_UTR
TTTCAAGGAAGATTACTTGC+AGG 0.393367 1_4:+697722 Msa0141930:five_prime_UTR
ATGGCAGGTGCAGACAAGTT+TGG 0.399313 1_4:+698306 Msa0141930:CDS
GCTGAATGGAGCTCCCAATA+AGG 0.400400 1_4:+698419 Msa0141930:CDS
GTTATGTCTTCAAATCGTTC+TGG 0.400887 1_4:+700254 Msa0141930:three_prime_UTR
GTGCTCAGGCTATATGATTT+GGG 0.405948 1_4:+697755 Msa0141930:five_prime_UTR
CCCGTCTATACTTGCAGTAA+TGG 0.412306 1_4:-698203 None:intergenic
CTTGCTCTATGTCAGTTTCA+AGG 0.423177 1_4:+697707 Msa0141930:five_prime_UTR
GATGAATTGGACAGGACCCC+TGG 0.427518 1_4:+699985 Msa0141930:CDS
GTTGTATATGGAACAGTTAT+GGG 0.433396 1_4:+698525 Msa0141930:CDS
TGCAAATCCATCGGCAATGT+TGG 0.438678 1_4:-699949 None:intergenic
CTACTGGTGGGAAGATCAAA+TGG 0.439184 1_4:+698385 Msa0141930:CDS
AGTTTCTTTAGTATCTCTCC+AGG 0.442693 1_4:-700003 None:intergenic
AGTTGTTGTGGCGTGGAGGC+TGG 0.448048 1_4:+700758 Msa0141930:three_prime_UTR
AAGACATAACCATTCAAAAC+GGG 0.451321 1_4:-700241 None:intergenic
ATGTGCTATATATTAGTAAG+AGG 0.453422 1_4:+700356 Msa0141930:three_prime_UTR
GGAAGATTACTTGCAGGAAT+CGG 0.455222 1_4:+697728 Msa0141930:five_prime_UTR
CAGGAATCGGACCTGTGCTC+AGG 0.471216 1_4:+697741 Msa0141930:five_prime_UTR
GCAAATCCATCGGCAATGTT+GGG 0.472137 1_4:-699948 None:intergenic
ATATATAGGTTTCTAGATGA+TGG 0.475130 1_4:+697638 Msa0141930:five_prime_UTR
TTTGTGTAGGATGTGATTGA+TGG 0.479418 1_4:+699907 Msa0141930:intron
CAAAAGGCTTCTCTTGTACC+AGG 0.493006 1_4:+698489 Msa0141930:CDS
CATCAGCAAATATGTAAAGC+TGG 0.494101 1_4:-698233 None:intergenic
ATGAATAGAGACAATGGAGC+TGG 0.496932 1_4:-697819 None:intergenic
GAAATTGTACAATTCCATGT+AGG 0.498723 1_4:+698447 Msa0141930:CDS
CTGATGATTCCGTTCCAAGA+TGG 0.499398 1_4:+698250 Msa0141930:five_prime_UTR
TCCATTACTGCAAGTATAGA+CGG 0.500792 1_4:+698202 Msa0141930:five_prime_UTR
GAAGACATAACCATTCAAAA+CGG 0.503701 1_4:-700242 None:intergenic
TGTGATCACAAGCTTGCAAA+AGG 0.507416 1_4:+698473 Msa0141930:CDS
GTTTCTTTAGTATCTCTCCA+GGG 0.513050 1_4:-700002 None:intergenic
TGGGAAGATCAAATGGGAGC+AGG 0.513392 1_4:+698392 Msa0141930:CDS
AAACTTGTCTGCACCTGCCA+TGG 0.513464 1_4:-698304 None:intergenic
CTGCAAAATGGCAAGTCAAA+AGG 0.516340 1_4:+700205 Msa0141930:three_prime_UTR
GCTTCTCTTGTACCAGGTGG+TGG 0.518034 1_4:+698495 Msa0141930:CDS
GGTTTAGAGTTGTTGTGGCG+TGG 0.521137 1_4:+700751 Msa0141930:three_prime_UTR
TTTGGAGATGCATATGAGAC+AGG 0.522575 1_4:+698605 Msa0141930:CDS
TATGACGCAGTAAGCCATCT+TGG 0.525927 1_4:-698264 None:intergenic
TTAACAAATGCTAGTTAGAG+AGG 0.528177 1_4:+700050 Msa0141930:exon
CTCCACAAAACTCAGGTTGA+GGG 0.530335 1_4:+697677 Msa0141930:five_prime_UTR
AAATTGTACAATTCCATGTA+GGG 0.530612 1_4:+698448 Msa0141930:CDS
CCCAAAAGGAGTTTAACTGC+TGG 0.535413 1_4:+697605 Msa0141930:five_prime_UTR
CCATTACTGCAAGTATAGAC+GGG 0.536221 1_4:+698203 Msa0141930:five_prime_UTR
TGGGAGCAGGGAAAGCTGAA+TGG 0.547867 1_4:+698405 Msa0141930:CDS
GGAACAGTTATGGGAAGTGT+TGG 0.550608 1_4:+698534 Msa0141930:CDS
TGTGCTATATATTAGTAAGA+GGG 0.550781 1_4:+700357 Msa0141930:three_prime_UTR
TTTCTTTAGTATCTCTCCAG+GGG 0.550975 1_4:-700001 None:intergenic
GATCACCGTTGGCATCTCCT+GGG 0.553304 1_4:+700296 Msa0141930:three_prime_UTR
GGGTGGGTTTAGAGTTGTTG+TGG 0.556125 1_4:+700746 Msa0141930:intron
TTGTGTAGGATGTGATTGAT+GGG 0.560234 1_4:+699908 Msa0141930:intron
TCTCCACAAAACTCAGGTTG+AGG 0.560791 1_4:+697676 Msa0141930:five_prime_UTR
TGAAACTGACATAGAGCAAG+AGG 0.561687 1_4:-697704 None:intergenic
AACGGTGATCACTTCAGCAC+AGG 0.562056 1_4:-700283 None:intergenic
GGGAAGATCAAATGGGAGCA+GGG 0.564796 1_4:+698393 Msa0141930:CDS
CGCAGTAAGCCATCTTGGAA+CGG 0.567865 1_4:-698259 None:intergenic
AGCTTATGTAGCAAATGCTG+TGG 0.568783 1_4:+700407 Msa0141930:three_prime_UTR
GCAATTCCCAACATTGCCGA+TGG 0.571083 1_4:+699942 Msa0141930:CDS
GAGATAGAAGAAGATCCTAC+TGG 0.571196 1_4:+698369 Msa0141930:CDS
AAATTGCTGATGAATTGGAC+AGG 0.573426 1_4:+699977 Msa0141930:CDS
TTGTGATCACATCCCCTACA+TGG 0.574600 1_4:-698461 None:intergenic
TACTGGTGGGAAGATCAAAT+GGG 0.578904 1_4:+698386 Msa0141930:CDS
TGAACTTCTCCACAAAACTC+AGG 0.580882 1_4:+697670 Msa0141930:five_prime_UTR
ACAACGCACTCTCCACCACC+TGG 0.599021 1_4:-698507 None:intergenic
AAATCATATAGCCTGAGCAC+AGG 0.604576 1_4:-697752 None:intergenic
CAGGACAATCCTCCGTTGTG+TGG 0.604924 1_4:+698624 Msa0141930:CDS
TGAATAGAGACAATGGAGCT+GGG 0.607678 1_4:-697818 None:intergenic
CATTTGATCTTCCCACCAGT+AGG 0.619370 1_4:-698384 None:intergenic
AGAGATACTAAAGAAACTTG+AGG 0.622153 1_4:+700008 Msa0141930:CDS
TACCGTGATCGAATTTATGT+AGG 0.623140 1_4:+697845 Msa0141930:five_prime_UTR
ATAGAAGAAGATCCTACTGG+TGG 0.623783 1_4:+698372 Msa0141930:CDS
ATAGGTTTCTAGATGATGGA+AGG 0.623931 1_4:+697642 Msa0141930:five_prime_UTR
TACATAAATTCGATCACGGT+AGG 0.629574 1_4:-697843 None:intergenic
AACAAAATCCAACCCACCAG+AGG 0.632656 1_4:-700794 None:intergenic
AATTTAACTACATAGCTACA+TGG 0.635371 1_4:-700455 None:intergenic
CACCTACATAAATTCGATCA+CGG 0.637531 1_4:-697847 None:intergenic
AATTGTACAATTCCATGTAG+GGG 0.643079 1_4:+698449 Msa0141930:CDS
TAGAAGAAGATCCTACTGGT+GGG 0.645752 1_4:+698373 Msa0141930:CDS
TGTGCTGAAGTGATCACCGT+TGG 0.650802 1_4:+700285 Msa0141930:three_prime_UTR
GTAGGCATGAATAGAGACAA+TGG 0.659123 1_4:-697825 None:intergenic
ATGTTGGGAATTGCTCACAT+AGG 0.661639 1_4:-699933 None:intergenic
TTAGAGTTGTTGTGGCGTGG+AGG 0.666785 1_4:+700754 Msa0141930:three_prime_UTR
ATCTCATCAGAAACGTCCTG+TGG 0.673716 1_4:-698351 None:intergenic
TGTGTAGGATGTGATTGATG+GGG 0.684029 1_4:+699909 Msa0141930:intron
CCATGGTGTCAAAATCTATG+TGG 0.684812 1_4:-698287 None:intergenic
AAGGCTTCTCTTGTACCAGG+TGG 0.698892 1_4:+698492 Msa0141930:CDS
GTTGTGTGGAAGAGACCACA+TGG 0.711547 1_4:+698638 Msa0141930:CDS
TGGTCTCTTCCACACAACGG+AGG 0.718300 1_4:-698633 None:intergenic
AGGCAGATCTATAAGCCATG+TGG 0.719537 1_4:-698653 None:intergenic
ATGTGGTCTCTTCCACACAA+CGG 0.726724 1_4:-698636 None:intergenic
TTATGTAGGTGACATCCAAG+AGG 0.727269 1_4:+697859 Msa0141930:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTAACTAATGTAAAT+TGG + chr1_4:698833-698852 Msa0141930:intron 10.0%
!!! TGTAAAATGTCTTTTTTTTA+GGG - chr1_4:697585-697604 None:intergenic 15.0%
!! GATTCTAATCTAATAAAAAT+AGG + chr1_4:698871-698890 Msa0141930:intron 15.0%
!!! TTTAATTTTTGAAAATTCTG+AGG + chr1_4:699876-699895 Msa0141930:intron 15.0%
!!! GTGTAAAATGTCTTTTTTTT+AGG - chr1_4:697586-697605 None:intergenic 20.0%
!!! TTGGAATTATACTACTATTT+TGG + chr1_4:698022-698041 Msa0141930:intron 20.0%
!!! TTTATTTTGTGAACAGAAAT+AGG - chr1_4:698142-698161 None:intergenic 20.0%
!! TTTCTTTCAGTTATTACAAA+TGG + chr1_4:698719-698738 Msa0141930:intron 20.0%
!!! TGTGTTAATGTTTTCATTAA+AGG - chr1_4:698781-698800 None:intergenic 20.0%
!!! ATTTTTGACATTTGAAACTT+TGG + chr1_4:698945-698964 Msa0141930:intron 20.0%
!!! TAGTGTTTTGTTTCAAATTA+TGG - chr1_4:699134-699153 None:intergenic 20.0%
!!! ATGGTTCATTAGATTTTATA+TGG + chr1_4:699548-699567 Msa0141930:intron 20.0%
!! ACATAATTAGACATTTAGTT+TGG - chr1_4:699768-699787 None:intergenic 20.0%
!! CATATTATTATTAACCTTGT+TGG + chr1_4:700481-700500 Msa0141930:intron 20.0%
! CTGGATTCATTCATATATAT+AGG + chr1_4:697624-697643 Msa0141930:five_prime_UTR 25.0%
!! ATATATAGGTTTCTAGATGA+TGG + chr1_4:697638-697657 Msa0141930:five_prime_UTR 25.0%
!! GTCTTTATTTGTTGAATATC+TGG + chr1_4:697901-697920 Msa0141930:intron 25.0%
! AATAGTAGTATAATTCCAAC+TGG - chr1_4:698021-698040 None:intergenic 25.0%
!!! TATTTTGGAAAACTAGACTA+AGG + chr1_4:698037-698056 Msa0141930:intron 25.0%
!!! TTGGAAATATCTTTTTTGCA+CGG + chr1_4:698325-698344 Msa0141930:CDS 25.0%
! ACAATTTCTTCAACCTTATT+GGG - chr1_4:698435-698454 None:intergenic 25.0%
! TACAATTTCTTCAACCTTAT+TGG - chr1_4:698436-698455 None:intergenic 25.0%
! AAATTGTACAATTCCATGTA+GGG + chr1_4:698448-698467 Msa0141930:CDS 25.0%
!!! TGTTGATTTCTTTTCTCATT+TGG + chr1_4:698587-698606 Msa0141930:CDS 25.0%
! ATATACCTTAACAGGAAAAT+AGG - chr1_4:698676-698695 None:intergenic 25.0%
! AGTTTAGTATATACCTTAAC+AGG - chr1_4:698684-698703 None:intergenic 25.0%
! AATGAACAAATGACACATTT+AGG - chr1_4:698804-698823 None:intergenic 25.0%
! TTGTACTAAATAACAGATCA+CGG - chr1_4:699064-699083 None:intergenic 25.0%
!!! TGAAGGCTTTTTATTTGAAT+AGG + chr1_4:699281-699300 Msa0141930:intron 25.0%
! AGATTTCAACAAACAAGTTT+AGG - chr1_4:699339-699358 None:intergenic 25.0%
!! TTTTATTAACCCTCTGTTAT+AGG + chr1_4:699374-699393 Msa0141930:intron 25.0%
!! GAAGATAATCCTTTTCATTT+TGG - chr1_4:699676-699695 None:intergenic 25.0%
! TTCATTCAAATGAGGAAAAA+GGG - chr1_4:699813-699832 None:intergenic 25.0%
! TTTCATTCAAATGAGGAAAA+AGG - chr1_4:699814-699833 None:intergenic 25.0%
!!! AGTTTTTTAATCTACGAACA+GGG + chr1_4:700146-700165 Msa0141930:intron 25.0%
! ATGTGCTATATATTAGTAAG+AGG + chr1_4:700356-700375 Msa0141930:three_prime_UTR 25.0%
! TGTGCTATATATTAGTAAGA+GGG + chr1_4:700357-700376 Msa0141930:three_prime_UTR 25.0%
! AATTTAACTACATAGCTACA+TGG - chr1_4:700458-700477 None:intergenic 25.0%
!!! TAGTTTGTATTTTTCTCTAC+TGG + chr1_4:700572-700591 Msa0141930:intron 25.0%
! AAAGACATTTTACACCCAAA+AGG + chr1_4:697591-697610 Msa0141930:five_prime_UTR 30.0%
ATTGATAGAAACATACCTCT+TGG - chr1_4:697877-697896 None:intergenic 30.0%
! CTGACAATTATTGTTTGCTA+TGG + chr1_4:697942-697961 Msa0141930:intron 30.0%
GAAATTGTACAATTCCATGT+AGG + chr1_4:698447-698466 Msa0141930:CDS 30.0%
AATTGTACAATTCCATGTAG+GGG + chr1_4:698449-698468 Msa0141930:CDS 30.0%
GTTGTATATGGAACAGTTAT+GGG + chr1_4:698525-698544 Msa0141930:CDS 30.0%
GTATAGCTAAGATACAAGAA+TGG - chr1_4:698908-698927 None:intergenic 30.0%
! AAACACTATTTTCTGTGATC+TGG + chr1_4:699146-699165 Msa0141930:intron 30.0%
! AACACTATTTTCTGTGATCT+GGG + chr1_4:699147-699166 Msa0141930:intron 30.0%
CATCTATAATGTGAAAGTGA+AGG + chr1_4:699264-699283 Msa0141930:intron 30.0%
!! GGCTTTTTATTTGAATAGGA+TGG + chr1_4:699285-699304 Msa0141930:intron 30.0%
! CAACAAACAAGTTTAGGATT+TGG - chr1_4:699333-699352 None:intergenic 30.0%
!! TGCTTTTGAGTGTATAAAAG+CGG + chr1_4:699413-699432 Msa0141930:intron 30.0%
! TTTGCTTTCCTTTGTGATTT+TGG + chr1_4:699450-699469 Msa0141930:intron 30.0%
!!! TCCTTTGTGATTTTGGTTTT+AGG + chr1_4:699457-699476 Msa0141930:intron 30.0%
!!! CCTTTGTGATTTTGGTTTTA+GGG + chr1_4:699458-699477 Msa0141930:intron 30.0%
TCAAGACTTTCATTCAAATG+AGG - chr1_4:699821-699840 None:intergenic 30.0%
! ATTTTTCTCTGCAAATCCAT+CGG - chr1_4:699961-699980 None:intergenic 30.0%
GAGAAAAATTGCTGATGAAT+TGG + chr1_4:699972-699991 Msa0141930:CDS 30.0%
AGAGATACTAAAGAAACTTG+AGG + chr1_4:700008-700027 Msa0141930:CDS 30.0%
TTAACAAATGCTAGTTAGAG+AGG + chr1_4:700050-700069 Msa0141930:exon 30.0%
!!! ATGGTTCCCTATTTTTGTTT+TGG + chr1_4:700074-700093 Msa0141930:intron 30.0%
!!! TGGTTCCCTATTTTTGTTTT+GGG + chr1_4:700075-700094 Msa0141930:intron 30.0%
AGTAGCCCAAAACAAAAATA+GGG - chr1_4:700083-700102 None:intergenic 30.0%
!!! CAGTTTTTTAATCTACGAAC+AGG + chr1_4:700145-700164 Msa0141930:intron 30.0%
!!! GTTTTTTAATCTACGAACAG+GGG + chr1_4:700147-700166 Msa0141930:intron 30.0%
!!! TTTTTTAATCTACGAACAGG+GGG + chr1_4:700148-700167 Msa0141930:intron 30.0%
TTTATAAGAGACCTGCAAAA+TGG + chr1_4:700193-700212 Msa0141930:three_prime_UTR 30.0%
!!! TTACTTTTTCCCGTTTTGAA+TGG + chr1_4:700232-700251 Msa0141930:three_prime_UTR 30.0%
AAGACATAACCATTCAAAAC+GGG - chr1_4:700244-700263 None:intergenic 30.0%
GAAGACATAACCATTCAAAA+CGG - chr1_4:700245-700264 None:intergenic 30.0%
GAATGAAAGAATAAAAACCC+AGG - chr1_4:700316-700335 None:intergenic 30.0%
TAATAATCTGAATGCCAACA+AGG - chr1_4:700498-700517 None:intergenic 30.0%
!!! GTATTTTTCTCTACTGGTTT+TGG + chr1_4:700578-700597 Msa0141930:intron 30.0%
!! ATAGGTTTCTAGATGATGGA+AGG + chr1_4:697642-697661 Msa0141930:five_prime_UTR 35.0%
TTTCAAGGAAGATTACTTGC+AGG + chr1_4:697722-697741 Msa0141930:five_prime_UTR 35.0%
TACATAAATTCGATCACGGT+AGG - chr1_4:697846-697865 None:intergenic 35.0%
TACCGTGATCGAATTTATGT+AGG + chr1_4:697845-697864 Msa0141930:five_prime_UTR 35.0%
CACCTACATAAATTCGATCA+CGG - chr1_4:697850-697869 None:intergenic 35.0%
TAACAAATGCCAACAGCAAA+AGG - chr1_4:697974-697993 None:intergenic 35.0%
TTTCTTAAGAATGCTCCAGT+TGG + chr1_4:698003-698022 Msa0141930:intron 35.0%
ACTAGACTAAGGCTTCTATA+AGG + chr1_4:698048-698067 Msa0141930:intron 35.0%
TCCATTACTGCAAGTATAGA+CGG + chr1_4:698202-698221 Msa0141930:five_prime_UTR 35.0%
CATCAGCAAATATGTAAAGC+TGG - chr1_4:698236-698255 None:intergenic 35.0%
CGTTGTATATGGAACAGTTA+TGG + chr1_4:698524-698543 Msa0141930:CDS 35.0%
! ATCTGCCTATTTTCCTGTTA+AGG + chr1_4:698668-698687 Msa0141930:CDS 35.0%
ATAAAAAGCCTCACAGAACA+AGG - chr1_4:698752-698771 None:intergenic 35.0%
AGTTCAGCGTTGTTAAATAG+CGG + chr1_4:698997-699016 Msa0141930:intron 35.0%
GACAGACTACATCATTGAAA+AGG - chr1_4:699245-699264 None:intergenic 35.0%
! ACAAGTTTAGGATTTGGTAG+AGG - chr1_4:699327-699346 None:intergenic 35.0%
CAGTATCAACCTATAACAGA+GGG - chr1_4:699386-699405 None:intergenic 35.0%
TCAGTATCAACCTATAACAG+AGG - chr1_4:699387-699406 None:intergenic 35.0%
CCCTAAAACCAAAATCACAA+AGG - chr1_4:699461-699480 None:intergenic 35.0%
ATTCTTGCTCCTCCAAATAT+TGG + chr1_4:699507-699526 Msa0141930:intron 35.0%
CTAAATCCACCAATATTTGG+AGG - chr1_4:699519-699538 None:intergenic 35.0%
CCTCTAAATCCACCAATATT+TGG - chr1_4:699522-699541 None:intergenic 35.0%
!! CCAAATATTGGTGGATTTAG+AGG + chr1_4:699519-699538 Msa0141930:intron 35.0%
AAATGCAACACAAATTTGCG+AGG + chr1_4:699595-699614 Msa0141930:intron 35.0%
! CTTTTCATTTTGGATCATCG+AGG - chr1_4:699666-699685 None:intergenic 35.0%
CTCGATGATCCAAAATGAAA+AGG + chr1_4:699664-699683 Msa0141930:intron 35.0%
GAGATAAGCCTATCTTTGTT+TGG - chr1_4:699731-699750 None:intergenic 35.0%
! TTAGTTTGGTTGAGAGAAGA+GGG - chr1_4:699754-699773 None:intergenic 35.0%
!! TTTAGTTTGGTTGAGAGAAG+AGG - chr1_4:699755-699774 None:intergenic 35.0%
TCAAATGAGGAAAAAGGGAT+GGG - chr1_4:699808-699827 None:intergenic 35.0%
TTCAAATGAGGAAAAAGGGA+TGG - chr1_4:699809-699828 None:intergenic 35.0%
CACATCCTACACAAATATGA+AGG - chr1_4:699902-699921 None:intergenic 35.0%
TTTGTGTAGGATGTGATTGA+TGG + chr1_4:699907-699926 Msa0141930:intron 35.0%
TTGTGTAGGATGTGATTGAT+GGG + chr1_4:699908-699927 Msa0141930:intron 35.0%
AAATTGCTGATGAATTGGAC+AGG + chr1_4:699977-699996 Msa0141930:CDS 35.0%
TTTCTTTAGTATCTCTCCAG+GGG - chr1_4:700004-700023 None:intergenic 35.0%
GTTTCTTTAGTATCTCTCCA+GGG - chr1_4:700005-700024 None:intergenic 35.0%
AGTTTCTTTAGTATCTCTCC+AGG - chr1_4:700006-700025 None:intergenic 35.0%
AAATGCTAGTTAGAGAGGTA+TGG + chr1_4:700055-700074 Msa0141930:intron 35.0%
CAGTAGCCCAAAACAAAAAT+AGG - chr1_4:700084-700103 None:intergenic 35.0%
GTTATGTCTTCAAATCGTTC+TGG + chr1_4:700254-700273 Msa0141930:three_prime_UTR 35.0%
TATTCGAAAGTGAATGGTAC+AGG - chr1_4:700522-700541 None:intergenic 35.0%
!! ATTGTTGTTTGCAGAGTGAT+CGG + chr1_4:700601-700620 Msa0141930:intron 35.0%
!!! TGTTTTTGGTCTGGTGTTTT+CGG + chr1_4:700657-700676 Msa0141930:intron 35.0%
! CCAGCAGTTAAACTCCTTTT+GGG - chr1_4:697608-697627 None:intergenic 40.0%
! TCCAGCAGTTAAACTCCTTT+TGG - chr1_4:697609-697628 None:intergenic 40.0%
TGAACTTCTCCACAAAACTC+AGG + chr1_4:697670-697689 Msa0141930:five_prime_UTR 40.0%
TGAAACTGACATAGAGCAAG+AGG - chr1_4:697707-697726 None:intergenic 40.0%
CTTGCTCTATGTCAGTTTCA+AGG + chr1_4:697707-697726 Msa0141930:five_prime_UTR 40.0%
GGAAGATTACTTGCAGGAAT+CGG + chr1_4:697728-697747 Msa0141930:five_prime_UTR 40.0%
AAATCATATAGCCTGAGCAC+AGG - chr1_4:697755-697774 None:intergenic 40.0%
TGTGCTCAGGCTATATGATT+TGG + chr1_4:697754-697773 Msa0141930:five_prime_UTR 40.0%
GTGCTCAGGCTATATGATTT+GGG + chr1_4:697755-697774 Msa0141930:five_prime_UTR 40.0%
TGAATAGAGACAATGGAGCT+GGG - chr1_4:697821-697840 None:intergenic 40.0%
ATGAATAGAGACAATGGAGC+TGG - chr1_4:697822-697841 None:intergenic 40.0%
GTAGGCATGAATAGAGACAA+TGG - chr1_4:697828-697847 None:intergenic 40.0%
TTATGTAGGTGACATCCAAG+AGG + chr1_4:697859-697878 Msa0141930:five_prime_UTR 40.0%
! ACTTGCAGTAATGGAAAGAC+TGG - chr1_4:698197-698216 None:intergenic 40.0%
CCATTACTGCAAGTATAGAC+GGG + chr1_4:698203-698222 Msa0141930:five_prime_UTR 40.0%
! CCATGGTGTCAAAATCTATG+TGG - chr1_4:698290-698309 None:intergenic 40.0%
!! CCACATAGATTTTGACACCA+TGG + chr1_4:698287-698306 Msa0141930:exon 40.0%
!! ATAGATTTTGACACCATGGC+AGG + chr1_4:698291-698310 Msa0141930:exon 40.0%
GAGATAGAAGAAGATCCTAC+TGG + chr1_4:698369-698388 Msa0141930:CDS 40.0%
ATAGAAGAAGATCCTACTGG+TGG + chr1_4:698372-698391 Msa0141930:CDS 40.0%
TAGAAGAAGATCCTACTGGT+GGG + chr1_4:698373-698392 Msa0141930:CDS 40.0%
TACTGGTGGGAAGATCAAAT+GGG + chr1_4:698386-698405 Msa0141930:CDS 40.0%
TGTGATCACAAGCTTGCAAA+AGG + chr1_4:698473-698492 Msa0141930:CDS 40.0%
TTTGGAGATGCATATGAGAC+AGG + chr1_4:698605-698624 Msa0141930:CDS 40.0%
TTGGTAGAGGTGAGACTATT+AGG - chr1_4:699314-699333 None:intergenic 40.0%
GGAGCAAGAATTGAATCAGA+AGG - chr1_4:699498-699517 None:intergenic 40.0%
! ATTTTGGATCATCGAGGAGA+GGG - chr1_4:699660-699679 None:intergenic 40.0%
! TCTTTGTTTGGTTCACAGGA+GGG - chr1_4:699719-699738 None:intergenic 40.0%
! ATCTTTGTTTGGTTCACAGG+AGG - chr1_4:699720-699739 None:intergenic 40.0%
! CCTATCTTTGTTTGGTTCAC+AGG - chr1_4:699723-699742 None:intergenic 40.0%
CCTGTGAACCAAACAAAGAT+AGG + chr1_4:699720-699739 Msa0141930:intron 40.0%
TAGTTTGGTTGAGAGAAGAG+GGG - chr1_4:699753-699772 None:intergenic 40.0%
!! AAAGGGATGGGTTAATTGGA+GGG - chr1_4:699796-699815 None:intergenic 40.0%
!! AAAAGGGATGGGTTAATTGG+AGG - chr1_4:699797-699816 None:intergenic 40.0%
!! GGAAAAAGGGATGGGTTAAT+TGG - chr1_4:699800-699819 None:intergenic 40.0%
TGAGGCCTTCATATTTGTGT+AGG + chr1_4:699894-699913 Msa0141930:intron 40.0%
TGTGTAGGATGTGATTGATG+GGG + chr1_4:699909-699928 Msa0141930:intron 40.0%
ATGTTGGGAATTGCTCACAT+AGG - chr1_4:699936-699955 None:intergenic 40.0%
!!! CTTTTGACTTGCCATTTTGC+AGG - chr1_4:700207-700226 None:intergenic 40.0%
CTGCAAAATGGCAAGTCAAA+AGG + chr1_4:700205-700224 Msa0141930:three_prime_UTR 40.0%
AGCTTATGTAGCAAATGCTG+TGG + chr1_4:700407-700426 Msa0141930:three_prime_UTR 40.0%
CCACTCTATTCGAAAGTGAA+TGG - chr1_4:700528-700547 None:intergenic 40.0%
CCATTCACTTTCGAATAGAG+TGG + chr1_4:700525-700544 Msa0141930:intron 40.0%
TACCTTATGAAACACTCTCC+CGG - chr1_4:700554-700573 None:intergenic 40.0%
CCCAAAAGGAGTTTAACTGC+TGG + chr1_4:697605-697624 Msa0141930:five_prime_UTR 45.0%
! TCTCCACAAAACTCAGGTTG+AGG + chr1_4:697676-697695 Msa0141930:five_prime_UTR 45.0%
!! CTCCACAAAACTCAGGTTGA+GGG + chr1_4:697677-697696 Msa0141930:five_prime_UTR 45.0%
CCCGTCTATACTTGCAGTAA+TGG - chr1_4:698206-698225 None:intergenic 45.0%
CTGATGATTCCGTTCCAAGA+TGG + chr1_4:698250-698269 Msa0141930:five_prime_UTR 45.0%
TATGACGCAGTAAGCCATCT+TGG - chr1_4:698267-698286 None:intergenic 45.0%
ATCTCATCAGAAACGTCCTG+TGG - chr1_4:698354-698373 None:intergenic 45.0%
CATTTGATCTTCCCACCAGT+AGG - chr1_4:698387-698406 None:intergenic 45.0%
CTACTGGTGGGAAGATCAAA+TGG + chr1_4:698385-698404 Msa0141930:CDS 45.0%
TTGTGATCACATCCCCTACA+TGG - chr1_4:698464-698483 None:intergenic 45.0%
CAAAAGGCTTCTCTTGTACC+AGG + chr1_4:698489-698508 Msa0141930:CDS 45.0%
! GGAACAGTTATGGGAAGTGT+TGG + chr1_4:698534-698553 Msa0141930:CDS 45.0%
ATGTGGTCTCTTCCACACAA+CGG - chr1_4:698639-698658 None:intergenic 45.0%
AGGCAGATCTATAAGCCATG+TGG - chr1_4:698656-698675 None:intergenic 45.0%
TGAACTGACAGCAGTGCAAA+AGG - chr1_4:698983-699002 None:intergenic 45.0%
GTGCTACAGTTCAGCAACAA+CGG - chr1_4:699102-699121 None:intergenic 45.0%
GTTGTTGCTGAACTGTAGCA+CGG + chr1_4:699101-699120 Msa0141930:intron 45.0%
CTTGCTCCTCCAAATATTGG+TGG + chr1_4:699510-699529 Msa0141930:intron 45.0%
GTGGATTTAGAGGAGAGCTA+TGG + chr1_4:699529-699548 Msa0141930:intron 45.0%
! TTTTGGATCATCGAGGAGAG+GGG - chr1_4:699659-699678 None:intergenic 45.0%
! CATTTTGGATCATCGAGGAG+AGG - chr1_4:699661-699680 None:intergenic 45.0%
!! AAGGGATGGGTTAATTGGAG+GGG - chr1_4:699795-699814 None:intergenic 45.0%
GCAAATCCATCGGCAATGTT+GGG - chr1_4:699951-699970 None:intergenic 45.0%
TGCAAATCCATCGGCAATGT+TGG - chr1_4:699952-699971 None:intergenic 45.0%
AAAAACCCAGGAGATGCCAA+CGG - chr1_4:700304-700323 None:intergenic 45.0%
CTTTCGAATAGAGTGGTGTC+CGG + chr1_4:700532-700551 Msa0141930:intron 45.0%
TTTCGAATAGAGTGGTGTCC+GGG + chr1_4:700533-700552 Msa0141930:intron 45.0%
! GCATGCTGTTGGTGTGTTTT+TGG + chr1_4:700643-700662 Msa0141930:intron 45.0%
!!! CTGTTGGTGTGTTTTTGGTC+TGG + chr1_4:700648-700667 Msa0141930:intron 45.0%
! TTTTCGGTTCTGTGGTTGTG+TGG + chr1_4:700673-700692 Msa0141930:intron 45.0%
! CACCCTCAACCTGAGTTTTG+TGG - chr1_4:697682-697701 None:intergenic 50.0%
!! TGGCTTCTGCCTTTTGCTGT+TGG + chr1_4:697962-697981 Msa0141930:intron 50.0%
CGCAGTAAGCCATCTTGGAA+CGG - chr1_4:698262-698281 None:intergenic 50.0%
AAACTTGTCTGCACCTGCCA+TGG - chr1_4:698307-698326 None:intergenic 50.0%
ATGGCAGGTGCAGACAAGTT+TGG + chr1_4:698306-698325 Msa0141930:CDS 50.0%
!!! CTTTTTTGCACGGTTGCCAC+AGG + chr1_4:698335-698354 Msa0141930:CDS 50.0%
TGGGAAGATCAAATGGGAGC+AGG + chr1_4:698392-698411 Msa0141930:CDS 50.0%
GGGAAGATCAAATGGGAGCA+GGG + chr1_4:698393-698412 Msa0141930:CDS 50.0%
GCTGAATGGAGCTCCCAATA+AGG + chr1_4:698419-698438 Msa0141930:CDS 50.0%
AAGGCTTCTCTTGTACCAGG+TGG + chr1_4:698492-698511 Msa0141930:CDS 50.0%
! GGTGGAGAGTGCGTTGTATA+TGG + chr1_4:698513-698532 Msa0141930:CDS 50.0%
GTTGTGTGGAAGAGACCACA+TGG + chr1_4:698638-698657 Msa0141930:CDS 50.0%
! GTTGCTTGCCTTGTTCTGTG+AGG + chr1_4:698741-698760 Msa0141930:intron 50.0%
AGGAGAGGGGCTAGAGTTAA+AGG - chr1_4:699646-699665 None:intergenic 50.0%
CAGGAGGGCGAATTTCATCA+AGG - chr1_4:699704-699723 None:intergenic 50.0%
!! GATGGGTTAATTGGAGGGGA+GGG - chr1_4:699791-699810 None:intergenic 50.0%
GCAATTCCCAACATTGCCGA+TGG + chr1_4:699942-699961 Msa0141930:CDS 50.0%
AACGGTGATCACTTCAGCAC+AGG - chr1_4:700286-700305 None:intergenic 50.0%
TGTGCTGAAGTGATCACCGT+TGG + chr1_4:700285-700304 Msa0141930:three_prime_UTR 50.0%
!! GTCCGGGAGAGTGTTTCATA+AGG + chr1_4:700549-700568 Msa0141930:intron 50.0%
!! TTTGCAGAGTGATCGGCTTG+AGG + chr1_4:700608-700627 Msa0141930:intron 50.0%
!! AGAGTGATCGGCTTGAGGTA+GGG + chr1_4:700613-700632 Msa0141930:intron 50.0%
! GTCTGGTGTTTTCGGTTCTG+TGG + chr1_4:700665-700684 Msa0141930:intron 50.0%
TGTGTGGAGTCTAGCGTGTT+TGG + chr1_4:700689-700708 Msa0141930:intron 50.0%
CCCACATACTAACTCACCGA+CGG - chr1_4:700729-700748 None:intergenic 50.0%
CCGTCGGTGAGTTAGTATGT+GGG + chr1_4:700726-700745 Msa0141930:intron 50.0%
TCGGTGAGTTAGTATGTGGG+TGG + chr1_4:700729-700748 Msa0141930:intron 50.0%
CGGTGAGTTAGTATGTGGGT+GGG + chr1_4:700730-700749 Msa0141930:intron 50.0%
! GGGTGGGTTTAGAGTTGTTG+TGG + chr1_4:700746-700765 Msa0141930:intron 50.0%
! GGTTTAGAGTTGTTGTGGCG+TGG + chr1_4:700751-700770 Msa0141930:three_prime_UTR 50.0%
TTAGAGTTGTTGTGGCGTGG+AGG + chr1_4:700754-700773 Msa0141930:three_prime_UTR 50.0%
!!! TGGAGGCTGGTTCTGTTTTC+AGG + chr1_4:700771-700790 Msa0141930:three_prime_UTR 50.0%
!! TGGTTCTGTTTTCAGGCCTC+TGG + chr1_4:700778-700797 Msa0141930:three_prime_UTR 50.0%
!! TTCTGTTTTCAGGCCTCTGG+TGG + chr1_4:700781-700800 Msa0141930:three_prime_UTR 50.0%
!! TCTGTTTTCAGGCCTCTGGT+GGG + chr1_4:700782-700801 Msa0141930:three_prime_UTR 50.0%
TGGGAGCAGGGAAAGCTGAA+TGG + chr1_4:698405-698424 Msa0141930:CDS 55.0%
GCTTCTCTTGTACCAGGTGG+TGG + chr1_4:698495-698514 Msa0141930:CDS 55.0%
CAGGACAATCCTCCGTTGTG+TGG + chr1_4:698624-698643 Msa0141930:CDS 55.0%
TGGTCTCTTCCACACAACGG+AGG - chr1_4:698636-698655 None:intergenic 55.0%
!! GGATGGGTTAATTGGAGGGG+AGG - chr1_4:699792-699811 None:intergenic 55.0%
GATGAATTGGACAGGACCCC+TGG + chr1_4:699985-700004 Msa0141930:CDS 55.0%
TGATCACCGTTGGCATCTCC+TGG + chr1_4:700295-700314 Msa0141930:three_prime_UTR 55.0%
GATCACCGTTGGCATCTCCT+GGG + chr1_4:700296-700315 Msa0141930:three_prime_UTR 55.0%
!! CAGAGTGATCGGCTTGAGGT+AGG + chr1_4:700612-700631 Msa0141930:intron 55.0%
! GGCTTTTGTGTTGTCGCCGT+CGG + chr1_4:700710-700729 Msa0141930:intron 55.0%
GCCGTCGGTGAGTTAGTATG+TGG + chr1_4:700725-700744 Msa0141930:intron 55.0%
!! TTTTCAGGCCTCTGGTGGGT+TGG + chr1_4:700786-700805 Msa0141930:three_prime_UTR 55.0%
CAGGAATCGGACCTGTGCTC+AGG + chr1_4:697741-697760 Msa0141930:five_prime_UTR 60.0%
ACAACGCACTCTCCACCACC+TGG - chr1_4:698510-698529 None:intergenic 60.0%
!! ATCGGCTTGAGGTAGGGTGC+AGG + chr1_4:700619-700638 Msa0141930:intron 60.0%
! TCGGCTTGAGGTAGGGTGCA+GGG + chr1_4:700620-700639 Msa0141930:intron 60.0%
AGTTGTTGTGGCGTGGAGGC+TGG + chr1_4:700758-700777 Msa0141930:three_prime_UTR 60.0%
CGGCTTGAGGTAGGGTGCAG+GGG + chr1_4:700621-700640 Msa0141930:intron 65.0%
! AGGGTGCAGGGGCATGCTGT+TGG + chr1_4:700632-700651 Msa0141930:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 697545 700814 697545 ID=Msa0141930;Name=Msa0141930
chr1_4 mRNA 697545 700814 697545 ID=Msa0141930-mRNA-1;Parent=Msa0141930;Name=Msa0141930-mRNA-1;_AED=0.07;_eAED=0.07;_QI=444|1|1|1|0.5|0.4|5|386|173
chr1_4 exon 697545 697880 697545 ID=Msa0141930-mRNA-1:exon:168;Parent=Msa0141930-mRNA-1
chr1_4 exon 698198 698689 698198 ID=Msa0141930-mRNA-1:exon:169;Parent=Msa0141930-mRNA-1
chr1_4 exon 699916 700071 699916 ID=Msa0141930-mRNA-1:exon:170;Parent=Msa0141930-mRNA-1
chr1_4 exon 700167 700466 700167 ID=Msa0141930-mRNA-1:exon:171;Parent=Msa0141930-mRNA-1
chr1_4 exon 700747 700814 700747 ID=Msa0141930-mRNA-1:exon:172;Parent=Msa0141930-mRNA-1
chr1_4 five_prime_UTR 697545 697880 697545 ID=Msa0141930-mRNA-1:five_prime_utr;Parent=Msa0141930-mRNA-1
chr1_4 five_prime_UTR 698198 698305 698198 ID=Msa0141930-mRNA-1:five_prime_utr;Parent=Msa0141930-mRNA-1
chr1_4 CDS 698306 698689 698306 ID=Msa0141930-mRNA-1:cds;Parent=Msa0141930-mRNA-1
chr1_4 CDS 699916 700053 699916 ID=Msa0141930-mRNA-1:cds;Parent=Msa0141930-mRNA-1
chr1_4 three_prime_UTR 700054 700071 700054 ID=Msa0141930-mRNA-1:three_prime_utr;Parent=Msa0141930-mRNA-1
chr1_4 three_prime_UTR 700167 700466 700167 ID=Msa0141930-mRNA-1:three_prime_utr;Parent=Msa0141930-mRNA-1
chr1_4 three_prime_UTR 700747 700814 700747 ID=Msa0141930-mRNA-1:three_prime_utr;Parent=Msa0141930-mRNA-1
Gene Sequence

>Msa0141930

ATGGCAGGTGCAGACAAGTTTGGAAATATCTTTTTTGCACGGTTGCCACAGGACGTTTCTGATGAGATAGAAGAAGATCCTACTGGTGGGAAGATCAAATGGGAGCAGGGAAAGCTGAATGGAGCTCCCAATAAGGTTGAAGAAATTGTACAATTCCATGTAGGGGATGTGATCACAAGCTTGCAAAAGGCTTCTCTTGTACCAGGTGGTGGAGAGTGCGTTGTATATGGAACAGTTATGGGAAGTGTTGGCGCATTACACGCGTTTACTTCACGAGATGATGTTGATTTCTTTTCTCATTTGGAGATGCATATGAGACAGGACAATCCTCCGTTGTGTGGAAGAGACCACATGGCTTATAGATCTGCCTATTTTCCTGTTAAGGATGTGATTGATGGGGACCTATGTGAGCAATTCCCAACATTGCCGATGGATTTGCAGAGAAAAATTGCTGATGAATTGGACAGGACCCCTGGAGAGATACTAAAGAAACTTGAGGAATTACGAAATAAGATTATTTAA
Protein sequence

>Msa0141930

MAGADKFGNIFFARLPQDVSDEIEEDPTGGKIKWEQGKLNGAPNKVEEIVQFHVGDVITSLQKASLVPGGGECVVYGTVMGSVGALHAFTSRDDVDFFSHLEMHMRQDNPPLCGRDHMAYRSAYFPVKDVIDGDLCEQFPTLPMDLQRKIADELDRTPGEILKKLEELRNKII*