AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0210960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 93 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAAATGGAAGTGTTAAT+TGG 0.162170 2_1:+51445614 None:intergenic
AATGATGTCAATGAGTTATT+TGG 0.185955 2_1:-51447934 Msa0210960:CDS
ATTTGAAAATTTCATATAAA+AGG 0.216554 2_1:+51446508 None:intergenic
TTAAATACCTCGATTTAAAA+TGG 0.250523 2_1:+51445601 None:intergenic
CTTGTGAACATTAAGAATTC+AGG 0.255126 2_1:-51446565 Msa0210960:three_prime_UTR
AGAATTGGACATTCACAAAA+TGG 0.255854 2_1:-51446268 Msa0210960:three_prime_UTR
TTCTTGCAGGGGAGCGGCTT+TGG 0.257739 2_1:-51447134 Msa0210960:intron
AAGAAGAAGGTTTGAATTGA+TGG 0.308391 2_1:+51445718 None:intergenic
GAATACTTACAACAACAATT+TGG 0.318130 2_1:+51445752 None:intergenic
TATTAGGAACAGGCAGAATA+TGG 0.324406 2_1:+51446308 None:intergenic
CTCTTCTAAGAGGAATTATT+AGG 0.335182 2_1:+51446292 None:intergenic
TCCTCTTAGAAGAGTAGAAT+TGG 0.345365 2_1:-51446283 Msa0210960:three_prime_UTR
TCAAATGTACCGTCGAATTC+AGG 0.346423 2_1:-51448098 Msa0210960:intron
CCGTCTGCTGAATTTGTTGC+TGG 0.348843 2_1:-51446745 Msa0210960:three_prime_UTR
TTTGAGGACACTGCTGCTTC+AGG 0.350191 2_1:-51447892 Msa0210960:CDS
CAGCTCTGAAGAACATTATC+AGG 0.356591 2_1:-51449530 Msa0210960:CDS
CTTCCAACAATTGCAGGTTA+TGG 0.369594 2_1:+51447793 None:intergenic
ATGATGTCAATGAGTTATTT+GGG 0.370649 2_1:-51447933 Msa0210960:CDS
TCAAACCTTCTTCTTGTACT+TGG 0.370919 2_1:-51445710 Msa0210960:three_prime_UTR
CGACGGTACATTTGAATCTT+CGG 0.385027 2_1:+51448106 None:intergenic
TAAGAGGAATTATTAGGAAC+AGG 0.396924 2_1:+51446298 None:intergenic
TGCAGCAGATCCTAAGTTGC+TGG 0.399151 2_1:-51447369 Msa0210960:three_prime_UTR
TAATGATGCTAGCAACTCTA+TGG 0.403205 2_1:-51447830 Msa0210960:CDS
TCATACCTTCCAACAATTGC+AGG 0.410987 2_1:+51447787 None:intergenic
GTCTTCTTCACTGGTCCTAC+AGG 0.415281 2_1:-51445984 Msa0210960:three_prime_UTR
GAATTGGACATTCACAAAAT+GGG 0.421927 2_1:-51446267 Msa0210960:three_prime_UTR
CTTGGAACTTCCCTACTTTG+TGG 0.430770 2_1:-51445692 Msa0210960:three_prime_UTR
ATTGAAATCCAGGGAAATCT+TGG 0.431539 2_1:-51446796 Msa0210960:intron
AATGCAACATGTGGGAATAT+TGG 0.431829 2_1:-51445810 Msa0210960:three_prime_UTR
TATATATCAGACATACCCTT+AGG 0.432727 2_1:-51448164 Msa0210960:intron
CTGATAATGTTCTTCAGAGC+TGG 0.435831 2_1:+51449531 None:intergenic
GTATTCTCTATTATTACAGT+TGG 0.447728 2_1:-51445843 Msa0210960:three_prime_UTR
TCCAATTCTACTCTTCTAAG+AGG 0.448706 2_1:+51446282 None:intergenic
TTCTCTATTATTACAGTTGG+AGG 0.450730 2_1:-51445840 Msa0210960:three_prime_UTR
ATACTCGCGACTTGGTTTAG+TGG 0.453344 2_1:-51446721 Msa0210960:three_prime_UTR
TAGCAACTCTATGGAGTCAT+TGG 0.453627 2_1:-51447821 Msa0210960:CDS
TTTCCAAACACCTACCAGTC+CGG 0.454107 2_1:+51447099 None:intergenic
TCTCTATGTGATGAAGAATT+CGG 0.459964 2_1:-51446653 Msa0210960:three_prime_UTR
AGACAAATTGTCTTCTTCAC+TGG 0.467398 2_1:-51445993 Msa0210960:three_prime_UTR
TTGTACACTAGCGGGACAAC+AGG 0.468065 2_1:-51446067 Msa0210960:three_prime_UTR
ATGGCGGAGACGGCGAGGTA+CGG 0.471966 2_1:+51449556 None:intergenic
AATCGAGGTATTTAACTAAG+TGG 0.480482 2_1:-51445593 Msa0210960:three_prime_UTR
ACTTCTACCACAACAACATC+AGG 0.481401 2_1:+51447496 None:intergenic
TAGCCATAACCTGCAATTGT+TGG 0.488861 2_1:-51447796 Msa0210960:three_prime_UTR
AATCTTGGTGGAGCTCGAAT+AGG 0.490823 2_1:-51446781 Msa0210960:three_prime_UTR
AACACCTACCAGTCCGGGAT+TGG 0.492471 2_1:+51447105 None:intergenic
CTTTGGATTAGGTGAAGATC+CGG 0.494848 2_1:-51446098 Msa0210960:intron
TTCCAAACACCTACCAGTCC+GGG 0.497287 2_1:+51447100 None:intergenic
TTGCTGGAATACTCGCGACT+TGG 0.497836 2_1:-51446729 Msa0210960:three_prime_UTR
AGGTACCAAGATAATGTCAA+TGG 0.498319 2_1:+51446528 None:intergenic
ATGTGGTTCTTGCAGGGGAG+CGG 0.504014 2_1:-51447140 Msa0210960:intron
TGCAATGCCACCACAAAGTA+GGG 0.510435 2_1:+51445682 None:intergenic
AAGTTCCAAGTACAAGAAGA+AGG 0.515161 2_1:+51445705 None:intergenic
TGATGTTGATAATGAATTTG+AGG 0.521778 2_1:-51447908 Msa0210960:CDS
TTGCAATGCCACCACAAAGT+AGG 0.522397 2_1:+51445681 None:intergenic
GGCAATCACCAATCCCGGAC+TGG 0.525150 2_1:-51447113 Msa0210960:intron
TACCCTTAGGGATGGATGGA+TGG 0.529376 2_1:-51448151 Msa0210960:CDS
TGATAATGTTCTTCAGAGCT+GGG 0.529587 2_1:+51449532 None:intergenic
TGTGACCAATGCCTAGGTGC+AGG 0.530298 2_1:+51447317 None:intergenic
GCTTTGGCAATCACCAATCC+CGG 0.538129 2_1:-51447118 Msa0210960:three_prime_UTR
GGAACTTCCCTACTTTGTGG+TGG 0.538669 2_1:-51445689 Msa0210960:three_prime_UTR
TTCAAAATGAAACTTACCTA+AGG 0.542574 2_1:-51446426 Msa0210960:three_prime_UTR
CCAATGCCTAGGTGCAGGTA+CGG 0.548490 2_1:+51447322 None:intergenic
ATGTTCTTCAGAGCTGGGTA+TGG 0.549422 2_1:+51449537 None:intergenic
AAGCCATCCATCCATCCCTA+AGG 0.550591 2_1:+51448148 None:intergenic
GACATACCCTTAGGGATGGA+TGG 0.550867 2_1:-51448155 Msa0210960:CDS
AGAGCTGGGTATGGCGGAGA+CGG 0.551603 2_1:+51449546 None:intergenic
TCTGCATAGTTGAGTGCAAG+TGG 0.553335 2_1:+51446685 None:intergenic
ATATATCAGACATACCCTTA+GGG 0.560336 2_1:-51448163 Msa0210960:intron
CCAGCAACAAATTCAGCAGA+CGG 0.563226 2_1:+51446745 None:intergenic
TCTCTATTATTACAGTTGGA+GGG 0.566642 2_1:-51445839 Msa0210960:three_prime_UTR
GAAATCCAGGGAAATCTTGG+TGG 0.568219 2_1:-51446793 Msa0210960:intron
CTCAAGACCTGATGTTGTTG+TGG 0.574621 2_1:-51447503 Msa0210960:intron
ATCAGACATACCCTTAGGGA+TGG 0.575259 2_1:-51448159 Msa0210960:intron
TTCTTCAGAGCTGGGTATGG+CGG 0.576008 2_1:+51449540 None:intergenic
CCGTACCTGCACCTAGGCAT+TGG 0.578210 2_1:-51447322 Msa0210960:three_prime_UTR
TGTGGTGGCATTGCAAACCT+TGG 0.582228 2_1:-51445674 Msa0210960:three_prime_UTR
CATAACCTGCAATTGTTGGA+AGG 0.584273 2_1:-51447792 Msa0210960:intron
CTGAAGAACATTATCAGGAT+CGG 0.593763 2_1:-51449525 Msa0210960:intron
GGGCTTCTTAATGCAACATG+TGG 0.594840 2_1:-51445819 Msa0210960:three_prime_UTR
ATATTGAGTACTGAAGATCA+TGG 0.599036 2_1:-51446376 Msa0210960:three_prime_UTR
CTAGTGTACAAAATCAGTGC+CGG 0.599653 2_1:+51446079 None:intergenic
ACATTAAACAGACAAAAGAA+AGG 0.602949 2_1:+51446592 None:intergenic
AATTGGACATTCACAAAATG+GGG 0.610443 2_1:-51446266 Msa0210960:three_prime_UTR
CTCGCGACTTGGTTTAGTGG+AGG 0.621339 2_1:-51446718 Msa0210960:three_prime_UTR
GGCTTCTTAATGCAACATGT+GGG 0.624011 2_1:-51445818 Msa0210960:three_prime_UTR
AGCCATCCATCCATCCCTAA+GGG 0.629828 2_1:+51448149 None:intergenic
TGGGTATGGCGGAGACGGCG+AGG 0.654911 2_1:+51449551 None:intergenic
GCGGAGACGGCGAGGTACGG+CGG 0.662251 2_1:+51449559 None:intergenic
TGGCATTGCAAACCTTGGAG+TGG 0.673161 2_1:-51445669 Msa0210960:three_prime_UTR
GTAACACCGTACCTGCACCT+AGG 0.692657 2_1:-51447328 Msa0210960:three_prime_UTR
TGATGTCAATGAGTTATTTG+GGG 0.714769 2_1:-51447932 Msa0210960:CDS
CGGCGGTGAGAAGAAGATCG+CGG 0.716171 2_1:+51449576 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAAAATTTCATATAAA+AGG + chr2_1:51448718-51448737 None:intergenic 10.0%
!!! AAAATTGTTTTAGTGATTTT+AGG - chr2_1:51445954-51445973 Msa0210960:three_prime_UTR 15.0%
!!! AAATTGTTTTAGTGATTTTA+GGG - chr2_1:51445955-51445974 Msa0210960:three_prime_UTR 15.0%
!! TGTAAATAACAAATAAGTAA+TGG + chr2_1:51446456-51446475 None:intergenic 15.0%
!!! CATCTAAATTTTCAATAAAA+GGG + chr2_1:51446483-51446502 None:intergenic 15.0%
!! ATATATATACAGCAAATATA+TGG + chr2_1:51447046-51447065 None:intergenic 15.0%
!!! TAGTAATATCATTTTCAAAT+TGG - chr2_1:51447531-51447550 Msa0210960:three_prime_UTR 15.0%
!!! ATTATCTTTGTTATATTGAT+TGG - chr2_1:51448174-51448193 Msa0210960:intron 15.0%
!!! TTATCTTTGTTATATTGATT+GGG - chr2_1:51448175-51448194 Msa0210960:intron 15.0%
!! TTTCAGAAAATTATGTGTTT+CGG - chr2_1:51445905-51445924 Msa0210960:three_prime_UTR 20.0%
!!! AAATCACTAAAACAATTTTC+TGG + chr2_1:51445953-51445972 None:intergenic 20.0%
!!! TGTATCTTTGATTTTAATTG+AGG - chr2_1:51446200-51446219 Msa0210960:intron 20.0%
!!! GTATCTTTGATTTTAATTGA+GGG - chr2_1:51446201-51446220 Msa0210960:intron 20.0%
!!! AAATTTTTTCACTTCTTTTG+TGG + chr2_1:51446294-51446313 None:intergenic 20.0%
!!! TTAGTCTGAGATAAAAATAA+TGG - chr2_1:51446335-51446354 Msa0210960:three_prime_UTR 20.0%
!!! TAGTCTGAGATAAAAATAAT+GGG - chr2_1:51446336-51446355 Msa0210960:three_prime_UTR 20.0%
!!! GAAGTATCTTTTGATTTTTT+TGG - chr2_1:51446363-51446382 Msa0210960:three_prime_UTR 20.0%
!!! CCATCTAAATTTTCAATAAA+AGG + chr2_1:51446484-51446503 None:intergenic 20.0%
!!! CCTTTTATTGAAAATTTAGA+TGG - chr2_1:51446481-51446500 Msa0210960:three_prime_UTR 20.0%
!! ATAAAACCCTAAATAAAAGA+GGG + chr2_1:51446781-51446800 None:intergenic 20.0%
!!! TTGAAGTTTAAAATGCTTTT+CGG - chr2_1:51447370-51447389 Msa0210960:three_prime_UTR 20.0%
!!! AATATCATTTTCAAATTGGT+TGG - chr2_1:51447535-51447554 Msa0210960:three_prime_UTR 20.0%
!! AGAAATAGTTTCAAATTGTT+GGG - chr2_1:51447762-51447781 Msa0210960:intron 20.0%
!!! TGTTTCATAATTTCATTTTC+AGG - chr2_1:51447818-51447837 Msa0210960:CDS 20.0%
!!! ATAATTTCATTTTCAGGTTT+GGG - chr2_1:51447824-51447843 Msa0210960:CDS 20.0%
!! AGGTTTATCTTATATATGTA+TGG - chr2_1:51448060-51448079 Msa0210960:intron 20.0%
!! ATCAATCTGCTAAAAATTTA+AGG - chr2_1:51448300-51448319 Msa0210960:intron 20.0%
!!! ATATGAAATTTTCAAATAGC+TGG - chr2_1:51448721-51448740 Msa0210960:intron 20.0%
!!! ACATTTTCAGCTTTTAAAAA+TGG - chr2_1:51449036-51449055 Msa0210960:intron 20.0%
!!! TTTAAAATGGAAGTGTTAAT+TGG + chr2_1:51449612-51449631 None:intergenic 20.0%
!! TTAAATACCTCGATTTAAAA+TGG + chr2_1:51449625-51449644 None:intergenic 20.0%
! TTGCATGTGTAAATTGAATT+AGG - chr2_1:51445763-51445782 Msa0210960:three_prime_UTR 25.0%
! TGCATGTGTAAATTGAATTA+GGG - chr2_1:51445764-51445783 Msa0210960:three_prime_UTR 25.0%
! TGTAAATTGAATTAGGGTTA+GGG - chr2_1:51445770-51445789 Msa0210960:three_prime_UTR 25.0%
!!! GGGTTTTTTGATTTTAATTG+AGG - chr2_1:51445818-51445837 Msa0210960:three_prime_UTR 25.0%
!!! GGTTTGTTTTAGTGATTTTA+GGG - chr2_1:51446009-51446028 Msa0210960:three_prime_UTR 25.0%
! AACAGTGAAACACAAAATTA+AGG + chr2_1:51446070-51446089 None:intergenic 25.0%
!!! TTTGCATTGATTAGTTGATT+TGG - chr2_1:51446092-51446111 Msa0210960:intron 25.0%
! ATTTGAGAGAGAAGAAATAA+TGG - chr2_1:51446310-51446329 Msa0210960:three_prime_UTR 25.0%
! TTTGAGAGAGAAGAAATAAT+GGG - chr2_1:51446311-51446330 Msa0210960:three_prime_UTR 25.0%
! TTGAAAATTTAGATGGACAA+TGG - chr2_1:51446488-51446507 Msa0210960:three_prime_UTR 25.0%
! CATAAAACCCTAAATAAAAG+AGG + chr2_1:51446782-51446801 None:intergenic 25.0%
!!! CTCTTTTATTTAGGGTTTTA+TGG - chr2_1:51446780-51446799 Msa0210960:three_prime_UTR 25.0%
! CACTGAAATAAATTGTATCA+CGG - chr2_1:51446870-51446889 Msa0210960:intron 25.0%
!! TCAGCTTTTATATATAACGA+TGG + chr2_1:51447182-51447201 None:intergenic 25.0%
! TCATTAGTGAACACTATAAT+TGG - chr2_1:51447202-51447221 Msa0210960:intron 25.0%
! AATGATGTCAATGAGTTATT+TGG - chr2_1:51447289-51447308 Msa0210960:intron 25.0%
! ATGATGTCAATGAGTTATTT+GGG - chr2_1:51447290-51447309 Msa0210960:intron 25.0%
!!! TGATGTTGATAATGAATTTG+AGG - chr2_1:51447315-51447334 Msa0210960:three_prime_UTR 25.0%
! ATTACTACTTCTGATAAAAG+TGG + chr2_1:51447518-51447537 None:intergenic 25.0%
! CAGAAATAGTTTCAAATTGT+TGG - chr2_1:51447761-51447780 Msa0210960:intron 25.0%
! ATGAAACATCATTTGCATAA+CGG + chr2_1:51447806-51447825 None:intergenic 25.0%
!!! CATAATTTCATTTTCAGGTT+TGG - chr2_1:51447823-51447842 Msa0210960:CDS 25.0%
!!! ACAAAAGAGAAATTTTTTGC+AGG - chr2_1:51447926-51447945 Msa0210960:CDS 25.0%
! CCTATAATAACTATCAAGTT+AGG + chr2_1:51447963-51447982 None:intergenic 25.0%
!! CCTAACTTGATAGTTATTAT+AGG - chr2_1:51447960-51447979 Msa0210960:intron 25.0%
! TGAAATTGATGAAGTCAATA+AGG - chr2_1:51448040-51448059 Msa0210960:intron 25.0%
! ATCTTATATATGTATGGATG+TGG - chr2_1:51448066-51448085 Msa0210960:intron 25.0%
! AATAAAAGAGAAACAACTCA+AGG + chr2_1:51448157-51448176 None:intergenic 25.0%
!! TGTTATATTGATTGGGAATA+TGG - chr2_1:51448182-51448201 Msa0210960:intron 25.0%
! TCGTTTAGTCAAATTATGTA+TGG - chr2_1:51448403-51448422 Msa0210960:intron 25.0%
! ACATTAAACAGACAAAAGAA+AGG + chr2_1:51448634-51448653 None:intergenic 25.0%
!! ATTTTCCATTGACATTATCT+TGG - chr2_1:51448690-51448709 Msa0210960:intron 25.0%
!! ATCATATATACTTTTCACGA+AGG + chr2_1:51448767-51448786 None:intergenic 25.0%
! TTCAAAATGAAACTTACCTA+AGG - chr2_1:51448797-51448816 Msa0210960:intron 25.0%
! TACCTAAGGAAAAAAAAAGT+TGG - chr2_1:51448811-51448830 Msa0210960:intron 25.0%
!!! AAACTGAGAAAAGGTTTTAT+TGG + chr2_1:51448893-51448912 None:intergenic 25.0%
! AATATGGAAAAACTGAGAAA+AGG + chr2_1:51448902-51448921 None:intergenic 25.0%
!!! CATTTGTTTTTCATATCCTT+TGG - chr2_1:51449108-51449127 Msa0210960:intron 25.0%
!! TTTTTCATATCCTTTGGATT+AGG - chr2_1:51449114-51449133 Msa0210960:intron 25.0%
!! GAACAATTTTATATACCTGT+AGG + chr2_1:51449257-51449276 None:intergenic 25.0%
! GTATTCTCTATTATTACAGT+TGG - chr2_1:51449380-51449399 Msa0210960:intron 25.0%
! GAATACTTACAACAACAATT+TGG + chr2_1:51449474-51449493 None:intergenic 25.0%
!! TGTTGTAAGTATTCTCTTTT+AGG - chr2_1:51449480-51449499 Msa0210960:intron 25.0%
!!! GAAGTGTTAATTGGATTTTA+GGG + chr2_1:51449603-51449622 None:intergenic 25.0%
TTTACACATGCAAATTCTGA+AGG + chr2_1:51445756-51445775 None:intergenic 30.0%
GTGTAAATTGAATTAGGGTT+AGG - chr2_1:51445769-51445788 Msa0210960:three_prime_UTR 30.0%
!!! TAATTGAGGTTTTGATTCCT+TGG - chr2_1:51445832-51445851 Msa0210960:three_prime_UTR 30.0%
TAAACAACAACACAAAACCA+AGG + chr2_1:51445852-51445871 None:intergenic 30.0%
!!! GATTTTAGGGTTTCATTTCT+TGG - chr2_1:51445968-51445987 Msa0210960:three_prime_UTR 30.0%
!!! TTGGTTTTGGGTTTGATTTT+AGG - chr2_1:51445987-51446006 Msa0210960:three_prime_UTR 30.0%
!!! TGGTTTTGGGTTTGATTTTA+GGG - chr2_1:51445988-51446007 Msa0210960:three_prime_UTR 30.0%
!!! GGGTTTGTTTTAGTGATTTT+AGG - chr2_1:51446008-51446027 Msa0210960:three_prime_UTR 30.0%
!! ATTAGTTGATTTGGAATGAC+AGG - chr2_1:51446101-51446120 Msa0210960:intron 30.0%
!! TTAGTTGATTTGGAATGACA+GGG - chr2_1:51446102-51446121 Msa0210960:intron 30.0%
!!! TTTGATTTTAATTGAGGGTC+TGG - chr2_1:51446206-51446225 Msa0210960:intron 30.0%
ACTTTCACAGTGATTTAGTT+AGG - chr2_1:51446662-51446681 Msa0210960:three_prime_UTR 30.0%
AAGAATAGTGAGAGTAGAAT+TGG - chr2_1:51446814-51446833 Msa0210960:intron 30.0%
TGAAATAAATTGTATCACGG+AGG - chr2_1:51446873-51446892 Msa0210960:intron 30.0%
TATATATCAGACATACCCTT+AGG - chr2_1:51447059-51447078 Msa0210960:intron 30.0%
ATATATCAGACATACCCTTA+GGG - chr2_1:51447060-51447079 Msa0210960:intron 30.0%
AAAAACATACCTGAATTCGA+CGG + chr2_1:51447137-51447156 None:intergenic 30.0%
!!! CAGGTATGTTTTTAGTCTAT+AGG - chr2_1:51447144-51447163 Msa0210960:intron 30.0%
!!! TTTTTTAGGCGTCGAAATTA+AGG - chr2_1:51447264-51447283 Msa0210960:intron 30.0%
TGATGTCAATGAGTTATTTG+GGG - chr2_1:51447291-51447310 Msa0210960:intron 30.0%
!!! TTCATTGCATCTTTTTTTGC+AGG - chr2_1:51447630-51447649 Msa0210960:intron 30.0%
AATGATGTAAACATGTCTGA+TGG - chr2_1:51447985-51448004 Msa0210960:intron 30.0%
TGTGTCTTGAATTTATGTTG+TGG - chr2_1:51448223-51448242 Msa0210960:intron 30.0%
ATGGAAAGCAGTTATCAAAA+AGG + chr2_1:51448250-51448269 None:intergenic 30.0%
!! TTGATAACTGCTTTCCATTT+TGG - chr2_1:51448252-51448271 Msa0210960:intron 30.0%
TTGAATACTTAGTCTATTGC+TGG - chr2_1:51448327-51448346 Msa0210960:intron 30.0%
TGAATACTTAGTCTATTGCT+GGG - chr2_1:51448328-51448347 Msa0210960:intron 30.0%
TCTCTATGTGATGAAGAATT+CGG - chr2_1:51448570-51448589 Msa0210960:intron 30.0%
CTTGTGAACATTAAGAATTC+AGG - chr2_1:51448658-51448677 Msa0210960:intron 30.0%
!!! CACCAACTTTTTTTTTCCTT+AGG + chr2_1:51448816-51448835 None:intergenic 30.0%
!! ATATTGAGTACTGAAGATCA+TGG - chr2_1:51448847-51448866 Msa0210960:intron 30.0%
TAAGAGGAATTATTAGGAAC+AGG + chr2_1:51448928-51448947 None:intergenic 30.0%
! CTCTTCTAAGAGGAATTATT+AGG + chr2_1:51448934-51448953 None:intergenic 30.0%
AGAATTGGACATTCACAAAA+TGG - chr2_1:51448955-51448974 Msa0210960:intron 30.0%
GAATTGGACATTCACAAAAT+GGG - chr2_1:51448956-51448975 Msa0210960:intron 30.0%
AATTGGACATTCACAAAATG+GGG - chr2_1:51448957-51448976 Msa0210960:intron 30.0%
! TTGATCTTTCGACATTTTTC+AGG + chr2_1:51449005-51449024 None:intergenic 30.0%
TTCTCTATTATTACAGTTGG+AGG - chr2_1:51449383-51449402 Msa0210960:intron 30.0%
TCTCTATTATTACAGTTGGA+GGG - chr2_1:51449384-51449403 Msa0210960:intron 30.0%
AAGAAGAAGGTTTGAATTGA+TGG + chr2_1:51449508-51449527 None:intergenic 30.0%
!!! GGAAGTGTTAATTGGATTTT+AGG + chr2_1:51449604-51449623 None:intergenic 30.0%
! AACACTTCCATTTTAAATCG+AGG - chr2_1:51449615-51449634 Msa0210960:five_prime_UTR 30.0%
! AATCGAGGTATTTAACTAAG+TGG - chr2_1:51449630-51449649 Msa0210960:five_prime_UTR 30.0%
! TGATAATGTTCTTCAGAGCT+GGG + chr2_1:51445694-51445713 None:intergenic 35.0%
CTGAAGAACATTATCAGGAT+CGG - chr2_1:51445698-51445717 Msa0210960:three_prime_UTR 35.0%
! TGCTTGCATGTTTTCAGAAA+AGG - chr2_1:51445797-51445816 Msa0210960:three_prime_UTR 35.0%
! GCTTGCATGTTTTCAGAAAA+GGG - chr2_1:51445798-51445817 Msa0210960:three_prime_UTR 35.0%
! AGGGTTTCATTTCTTGGTTT+TGG - chr2_1:51445974-51445993 Msa0210960:three_prime_UTR 35.0%
!! GGGTTTCATTTCTTGGTTTT+GGG - chr2_1:51445975-51445994 Msa0210960:three_prime_UTR 35.0%
!! TAGTTGATTTGGAATGACAG+GGG - chr2_1:51446103-51446122 Msa0210960:intron 35.0%
TTTGACGATGCTAATGTTTG+TGG - chr2_1:51446146-51446165 Msa0210960:intron 35.0%
! TTAGATGGACAATGGATTTG+AGG - chr2_1:51446496-51446515 Msa0210960:three_prime_UTR 35.0%
! ACAAGCAGCTTTTGATATGA+TGG - chr2_1:51446523-51446542 Msa0210960:three_prime_UTR 35.0%
CAGTCAACAGAGGAAAAAAA+GGG - chr2_1:51446550-51446569 Msa0210960:three_prime_UTR 35.0%
ATGCTTCTGATCCTTTGATT+TGG - chr2_1:51446741-51446760 Msa0210960:three_prime_UTR 35.0%
! TGCTTCTGATCCTTTGATTT+GGG - chr2_1:51446742-51446761 Msa0210960:three_prime_UTR 35.0%
AAATAAAAGAGGGCTCATCA+AGG + chr2_1:51446771-51446790 None:intergenic 35.0%
!! TGATGAGCCCTCTTTTATTT+AGG - chr2_1:51446771-51446790 Msa0210960:three_prime_UTR 35.0%
!! GATGAGCCCTCTTTTATTTA+GGG - chr2_1:51446772-51446791 Msa0210960:three_prime_UTR 35.0%
CGGAGGAAACAATCAATATT+AGG - chr2_1:51446890-51446909 Msa0210960:intron 35.0%
TAATGATGCTAGCAACTCTA+TGG - chr2_1:51447393-51447412 Msa0210960:intron 35.0%
! CGTTGTCTAATTTTGCGAAT+TGG + chr2_1:51447667-51447686 None:intergenic 35.0%
TTCAAAAACACCAGCAACTT+AGG + chr2_1:51447867-51447886 None:intergenic 35.0%
CAAGAAACCTCATTCCAAAA+TGG + chr2_1:51448269-51448288 None:intergenic 35.0%
!!! CATTTTGGAATGAGGTTTCT+TGG - chr2_1:51448267-51448286 Msa0210960:intron 35.0%
! TATGTATGGCATTGAAATCC+AGG - chr2_1:51448417-51448436 Msa0210960:intron 35.0%
! ATGTATGGCATTGAAATCCA+GGG - chr2_1:51448418-51448437 Msa0210960:intron 35.0%
ATTGAAATCCAGGGAAATCT+TGG - chr2_1:51448427-51448446 Msa0210960:intron 35.0%
AGGTACCAAGATAATGTCAA+TGG + chr2_1:51448698-51448717 None:intergenic 35.0%
TATTAGGAACAGGCAGAATA+TGG + chr2_1:51448918-51448937 None:intergenic 35.0%
TCCTCTTAGAAGAGTAGAAT+TGG - chr2_1:51448940-51448959 Msa0210960:intron 35.0%
TCCAATTCTACTCTTCTAAG+AGG + chr2_1:51448944-51448963 None:intergenic 35.0%
ATTGCCTTGGAAAACTGATA+CGG + chr2_1:51449078-51449097 None:intergenic 35.0%
ATGATGAGAAAGAATTGCCT+TGG + chr2_1:51449091-51449110 None:intergenic 35.0%
AGACAAATTGTCTTCTTCAC+TGG - chr2_1:51449230-51449249 Msa0210960:intron 35.0%
AATGCAACATGTGGGAATAT+TGG - chr2_1:51449413-51449432 Msa0210960:intron 35.0%
TCAAACCTTCTTCTTGTACT+TGG - chr2_1:51449513-51449532 Msa0210960:intron 35.0%
AAGTTCCAAGTACAAGAAGA+AGG + chr2_1:51449521-51449540 None:intergenic 35.0%
!! CATATTTTTGTTCCACTCCA+AGG + chr2_1:51449569-51449588 None:intergenic 35.0%
! CTGATAATGTTCTTCAGAGC+TGG + chr2_1:51445695-51445714 None:intergenic 40.0%
!! CAGCTCTGAAGAACATTATC+AGG - chr2_1:51445693-51445712 Msa0210960:three_prime_UTR 40.0%
!!! AGGGTCTGGTCTTTTGATTT+TGG - chr2_1:51446220-51446239 Msa0210960:intron 40.0%
!!! GGGTCTGGTCTTTTGATTTT+GGG - chr2_1:51446221-51446240 Msa0210960:three_prime_UTR 40.0%
! ATGGACAATGGATTTGAGGA+TGG - chr2_1:51446500-51446519 Msa0210960:three_prime_UTR 40.0%
GCAGTCAACAGAGGAAAAAA+AGG - chr2_1:51446549-51446568 Msa0210960:three_prime_UTR 40.0%
AATCACTGTGAAAGTGAGAG+TGG + chr2_1:51446657-51446676 None:intergenic 40.0%
ATCAAGGACTCCCAAATCAA+AGG + chr2_1:51446755-51446774 None:intergenic 40.0%
CGACGGTACATTTGAATCTT+CGG + chr2_1:51447120-51447139 None:intergenic 40.0%
TCAAATGTACCGTCGAATTC+AGG - chr2_1:51447125-51447144 Msa0210960:three_prime_UTR 40.0%
TAGCAACTCTATGGAGTCAT+TGG - chr2_1:51447402-51447421 Msa0210960:intron 40.0%
TAGCCATAACCTGCAATTGT+TGG - chr2_1:51447427-51447446 Msa0210960:intron 40.0%
CTTCCAACAATTGCAGGTTA+TGG + chr2_1:51447433-51447452 None:intergenic 40.0%
CATAACCTGCAATTGTTGGA+AGG - chr2_1:51447431-51447450 Msa0210960:intron 40.0%
TCATACCTTCCAACAATTGC+AGG + chr2_1:51447439-51447458 None:intergenic 40.0%
! GCAATTGTTGGAAGGTATGA+TGG - chr2_1:51447439-51447458 Msa0210960:intron 40.0%
ACTTCTACCACAACAACATC+AGG + chr2_1:51447730-51447749 None:intergenic 40.0%
ATTAGTACATCACACACGCA+AGG + chr2_1:51448016-51448035 None:intergenic 40.0%
TTGCGTGTGTGATGTACTAA+TGG - chr2_1:51448015-51448034 Msa0210960:intron 40.0%
TGCGTGTGTGATGTACTAAT+GGG - chr2_1:51448016-51448035 Msa0210960:intron 40.0%
ACTGGTAGGTGTTTGGAAAT+GGG - chr2_1:51448128-51448147 Msa0210960:CDS 40.0%
!!! CTGCTTTCCATTTTGGAATG+AGG - chr2_1:51448259-51448278 Msa0210960:intron 40.0%
! ATCTCCGTATCAGTTTTCCA+AGG - chr2_1:51449071-51449090 Msa0210960:intron 40.0%
! CTTTGGATTAGGTGAAGATC+CGG - chr2_1:51449125-51449144 Msa0210960:intron 40.0%
CTAGTGTACAAAATCAGTGC+CGG + chr2_1:51449147-51449166 None:intergenic 40.0%
!!! GCACTGATTTTGTACACTAG+CGG - chr2_1:51449147-51449166 Msa0210960:intron 40.0%
!!! CACTGATTTTGTACACTAGC+GGG - chr2_1:51449148-51449167 Msa0210960:intron 40.0%
GGCTTCTTAATGCAACATGT+GGG - chr2_1:51449405-51449424 Msa0210960:intron 40.0%
ATGTTCTTCAGAGCTGGGTA+TGG + chr2_1:51445689-51445708 None:intergenic 45.0%
GAACATTATCAGGATCGGTG+AGG - chr2_1:51445703-51445722 Msa0210960:three_prime_UTR 45.0%
TGTGAAAGTGAGAGTGGTGA+TGG + chr2_1:51446651-51446670 None:intergenic 45.0%
ATCAGACATACCCTTAGGGA+TGG - chr2_1:51447064-51447083 Msa0210960:intron 45.0%
CTCAAGACCTGATGTTGTTG+TGG - chr2_1:51447720-51447739 Msa0210960:intron 45.0%
! TTGGGTATCCGCTTCTTTTG+AGG - chr2_1:51447780-51447799 Msa0210960:intron 45.0%
CATAACGGCCTCAAAAGAAG+CGG + chr2_1:51447791-51447810 None:intergenic 45.0%
! CTCTTTTGTGACCAATGCCT+AGG + chr2_1:51447915-51447934 None:intergenic 45.0%
GCGTGTGTGATGTACTAATG+GGG - chr2_1:51448017-51448036 Msa0210960:intron 45.0%
TGTATGGATGTGGTTCTTGC+AGG - chr2_1:51448076-51448095 Msa0210960:intron 45.0%
GTATGGATGTGGTTCTTGCA+GGG - chr2_1:51448077-51448096 Msa0210960:intron 45.0%
TATGGATGTGGTTCTTGCAG+GGG - chr2_1:51448078-51448097 Msa0210960:intron 45.0%
TTTCCAAACACCTACCAGTC+CGG + chr2_1:51448127-51448146 None:intergenic 45.0%
GACTGGTAGGTGTTTGGAAA+TGG - chr2_1:51448127-51448146 Msa0210960:CDS 45.0%
GAAATCCAGGGAAATCTTGG+TGG - chr2_1:51448430-51448449 Msa0210960:intron 45.0%
AATCTTGGTGGAGCTCGAAT+AGG - chr2_1:51448442-51448461 Msa0210960:intron 45.0%
CCAGCAACAAATTCAGCAGA+CGG + chr2_1:51448481-51448500 None:intergenic 45.0%
!! ATACTCGCGACTTGGTTTAG+TGG - chr2_1:51448502-51448521 Msa0210960:intron 45.0%
TCTGCATAGTTGAGTGCAAG+TGG + chr2_1:51448541-51448560 None:intergenic 45.0%
CGGATCTTCACCTAATCCAA+AGG + chr2_1:51449127-51449146 None:intergenic 45.0%
GGGCTTCTTAATGCAACATG+TGG - chr2_1:51449404-51449423 Msa0210960:intron 45.0%
CTTGGAACTTCCCTACTTTG+TGG - chr2_1:51449531-51449550 Msa0210960:CDS 45.0%
TGCAATGCCACCACAAAGTA+GGG + chr2_1:51449544-51449563 None:intergenic 45.0%
TTGCAATGCCACCACAAAGT+AGG + chr2_1:51449545-51449564 None:intergenic 45.0%
!!! TAATTAGATATTATTTTTTT+AGG - chr2_1:51447250-51447269 Msa0210960:intron 5.0%
TTCTTCAGAGCTGGGTATGG+CGG + chr2_1:51445686-51445705 None:intergenic 50.0%
TGATGGCAAGCAGTCAACAG+AGG - chr2_1:51446540-51446559 Msa0210960:three_prime_UTR 50.0%
!! GAAAGTGAGAGTGGTGATGG+AGG + chr2_1:51446648-51446667 None:intergenic 50.0%
GACATACCCTTAGGGATGGA+TGG - chr2_1:51447068-51447087 Msa0210960:intron 50.0%
TACCCTTAGGGATGGATGGA+TGG - chr2_1:51447072-51447091 Msa0210960:intron 50.0%
AGCCATCCATCCATCCCTAA+GGG + chr2_1:51447077-51447096 None:intergenic 50.0%
AAGCCATCCATCCATCCCTA+AGG + chr2_1:51447078-51447097 None:intergenic 50.0%
! TTTGAGGACACTGCTGCTTC+AGG - chr2_1:51447331-51447350 Msa0210960:three_prime_UTR 50.0%
TGCAGCAGATCCTAAGTTGC+TGG - chr2_1:51447854-51447873 Msa0210960:CDS 50.0%
GCTTTGGCAATCACCAATCC+CGG - chr2_1:51448105-51448124 Msa0210960:CDS 50.0%
TTCCAAACACCTACCAGTCC+GGG + chr2_1:51448126-51448145 None:intergenic 50.0%
!! CCGTCTGCTGAATTTGTTGC+TGG - chr2_1:51448478-51448497 Msa0210960:intron 50.0%
TTGCTGGAATACTCGCGACT+TGG - chr2_1:51448494-51448513 Msa0210960:intron 50.0%
TTGTACACTAGCGGGACAAC+AGG - chr2_1:51449156-51449175 Msa0210960:intron 50.0%
GTCTTCTTCACTGGTCCTAC+AGG - chr2_1:51449239-51449258 Msa0210960:intron 50.0%
GGAACTTCCCTACTTTGTGG+TGG - chr2_1:51449534-51449553 Msa0210960:CDS 50.0%
TGTGGTGGCATTGCAAACCT+TGG - chr2_1:51449549-51449568 Msa0210960:CDS 50.0%
TGGCATTGCAAACCTTGGAG+TGG - chr2_1:51449554-51449573 Msa0210960:CDS 50.0%
GTAACACCGTACCTGCACCT+AGG - chr2_1:51447895-51447914 Msa0210960:CDS 55.0%
CCAATGCCTAGGTGCAGGTA+CGG + chr2_1:51447904-51447923 None:intergenic 55.0%
TGTGACCAATGCCTAGGTGC+AGG + chr2_1:51447909-51447928 None:intergenic 55.0%
ATGTGGTTCTTGCAGGGGAG+CGG - chr2_1:51448083-51448102 Msa0210960:intron 55.0%
!! ATCACCAATCCCGGACTGGT+AGG - chr2_1:51448114-51448133 Msa0210960:CDS 55.0%
AACACCTACCAGTCCGGGAT+TGG + chr2_1:51448121-51448140 None:intergenic 55.0%
!! ATCCCGGACTGGTAGGTGTT+TGG - chr2_1:51448121-51448140 Msa0210960:CDS 55.0%
GAGCTCCACCAAGATTTCCC+TGG + chr2_1:51448438-51448457 None:intergenic 55.0%
!! CTCGCGACTTGGTTTAGTGG+AGG - chr2_1:51448505-51448524 Msa0210960:intron 55.0%
CGGCGGTGAGAAGAAGATCG+CGG + chr2_1:51445650-51445669 None:intergenic 60.0%
! AGAGCTGGGTATGGCGGAGA+CGG + chr2_1:51445680-51445699 None:intergenic 60.0%
CCGTACCTGCACCTAGGCAT+TGG - chr2_1:51447901-51447920 Msa0210960:CDS 60.0%
TTCTTGCAGGGGAGCGGCTT+TGG - chr2_1:51448089-51448108 Msa0210960:intron 60.0%
GGCAATCACCAATCCCGGAC+TGG - chr2_1:51448110-51448129 Msa0210960:CDS 60.0%
! ATGGCGGAGACGGCGAGGTA+CGG + chr2_1:51445670-51445689 None:intergenic 65.0%
! TGGGTATGGCGGAGACGGCG+AGG + chr2_1:51445675-51445694 None:intergenic 70.0%
GCGGAGACGGCGAGGTACGG+CGG + chr2_1:51445667-51445686 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 51445587 51449658 51445587 ID=Msa0210960;Name=Msa0210960
chr2_1 mRNA 51445587 51449658 51445587 ID=Msa0210960-mRNA-1;Parent=Msa0210960;Name=Msa0210960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=45|0.85|1|1|0.57|0.62|8|1364|107
chr2_1 exon 51445587 51446109 51445587 ID=Msa0210960-mRNA-1:exon:14357;Parent=Msa0210960-mRNA-1
chr2_1 exon 51446221 51446806 51446221 ID=Msa0210960-mRNA-1:exon:14356;Parent=Msa0210960-mRNA-1
chr2_1 exon 51447114 51447147 51447114 ID=Msa0210960-mRNA-1:exon:14355;Parent=Msa0210960-mRNA-1
chr2_1 exon 51447298 51447405 51447298 ID=Msa0210960-mRNA-1:exon:14354;Parent=Msa0210960-mRNA-1
chr2_1 exon 51447504 51447593 51447504 ID=Msa0210960-mRNA-1:exon:14353;Parent=Msa0210960-mRNA-1
chr2_1 exon 51447793 51447973 51447793 ID=Msa0210960-mRNA-1:exon:14352;Parent=Msa0210960-mRNA-1
chr2_1 exon 51448099 51448176 51448099 ID=Msa0210960-mRNA-1:exon:14351;Parent=Msa0210960-mRNA-1
chr2_1 exon 51449526 51449658 51449526 ID=Msa0210960-mRNA-1:exon:14350;Parent=Msa0210960-mRNA-1
chr2_1 five_prime_UTR 51449614 51449658 51449614 ID=Msa0210960-mRNA-1:five_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 CDS 51449526 51449613 51449526 ID=Msa0210960-mRNA-1:cds;Parent=Msa0210960-mRNA-1
chr2_1 CDS 51448099 51448176 51448099 ID=Msa0210960-mRNA-1:cds;Parent=Msa0210960-mRNA-1
chr2_1 CDS 51447816 51447973 51447816 ID=Msa0210960-mRNA-1:cds;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51447793 51447815 51447793 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51447504 51447593 51447504 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51447298 51447405 51447298 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51447114 51447147 51447114 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51446221 51446806 51446221 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
chr2_1 three_prime_UTR 51445587 51446109 51445587 ID=Msa0210960-mRNA-1:three_prime_utr;Parent=Msa0210960-mRNA-1
Gene Sequence

>Msa0210960

ATGATACTCTCTCAGCCGCGATCTTCTTCTCACCGCCGTACCTCGCCGTCTCCGCCATACCCAGCTCTGAAGAACATTATCAGGATCGACATACCCTTAGGGATGGATGGATGGCTTATTTGCTGTATATATAATGCCGAAGATTCAAATGTACCGTCGAATTCAGGCGTCGAAATTAAGGTGAATGATGTCAATGAGTTATTTGGGGCTGATGTTGATAATGAATTTGAGGACACTGCTGCTTCAGGTAAATCTAACAATGAATTGAAGTTTAAAATGCTTTTCGGTAATGATGCTAGCAACTCTATGGAGTCATTGGCATAG
Protein sequence

>Msa0210960

MILSQPRSSSHRRTSPSPPYPALKNIIRIDIPLGMDGWLICCIYNAEDSNVPSNSGVEIKVNDVNELFGADVDNEFEDTAASGKSNNELKFKMLFGNDASNSMESLA*