AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0213070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 92 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAAATGGAAGTGTTAAT+TGG 0.162170 2_1:+55624276 None:intergenic
AATGATGTCAATGAGTTATT+TGG 0.185955 2_1:-55626753 Msa0213070:CDS
ATTTGAAAATTTCATATAAA+AGG 0.216554 2_1:+55625170 None:intergenic
TTAAATACCTCGATTTAAAA+TGG 0.250523 2_1:+55624263 None:intergenic
AGAATTGGACATTCACAAAA+TGG 0.255854 2_1:-55624930 Msa0213070:three_prime_UTR
TTCTTGCAGGGGAGCGGCTT+TGG 0.257739 2_1:-55625953 Msa0213070:intron
TTTAGCTTGTGAAAGAATTC+AGG 0.267726 2_1:-55625227 Msa0213070:three_prime_UTR
TATTAGGAACAGACAGAATA+TGG 0.281465 2_1:+55624970 None:intergenic
GAATACTTACAACAACAATT+TGG 0.318130 2_1:+55624414 None:intergenic
CTCTTCTAAGAGGAATTATT+AGG 0.335182 2_1:+55624954 None:intergenic
TCCTCTTAGAAGAGTAGAAT+TGG 0.345365 2_1:-55624945 Msa0213070:three_prime_UTR
TCAAATGTACCGTCGAATTC+AGG 0.346423 2_1:-55626917 Msa0213070:intron
CCGTCTGCTGAATTTGTTGC+TGG 0.348843 2_1:-55625402 Msa0213070:three_prime_UTR
TTTGAGGACACTGCTGCTTC+AGG 0.350191 2_1:-55626711 Msa0213070:CDS
CAGCTCTGAAGAACATTATC+AGG 0.356591 2_1:-55628350 Msa0213070:CDS
CTTCCAACAATTGCAGGTTA+TGG 0.369594 2_1:+55626612 None:intergenic
ATGATGTCAATGAGTTATTT+GGG 0.370649 2_1:-55626752 Msa0213070:CDS
TCAAACCTTCTTCTTGTGCT+TGG 0.370945 2_1:-55624372 Msa0213070:three_prime_UTR
CGACGGTACATTTGAATCTT+CGG 0.385027 2_1:+55626925 None:intergenic
TGCAGCAGATCCTAAGTTGC+TGG 0.399151 2_1:-55626188 Msa0213070:three_prime_UTR
TAATGATGCTAGCAACTCTA+TGG 0.403205 2_1:-55626649 Msa0213070:CDS
TCATACCTTCCAACAATTGC+AGG 0.410987 2_1:+55626606 None:intergenic
GTCTTCTTCACTGGTCCTAC+AGG 0.415281 2_1:-55624646 Msa0213070:three_prime_UTR
TTGTACACTAGCGGGACAAT+AGG 0.416640 2_1:-55624729 Msa0213070:three_prime_UTR
ATACTCTTGACTTGGTTTAG+TGG 0.420631 2_1:-55625378 Msa0213070:three_prime_UTR
GAATTGGACATTCACAAAAT+GGG 0.421927 2_1:-55624929 Msa0213070:three_prime_UTR
ATTGAAATCCAGGGAAATCT+TGG 0.431539 2_1:-55625453 Msa0213070:intron
AATGCAACATGTGGGAATAT+TGG 0.431829 2_1:-55624472 Msa0213070:three_prime_UTR
CTTGGAACTTCCCTACTTTG+TGG 0.435724 2_1:-55624354 Msa0213070:three_prime_UTR
CTGATAATGTTCTTCAGAGC+TGG 0.435831 2_1:+55628351 Msa0213080:CDS
GTATTCTCTATTATTACAGT+TGG 0.447728 2_1:-55624505 Msa0213070:three_prime_UTR
TATATATCAGACATGCCCTT+AGG 0.448684 2_1:-55626983 Msa0213070:intron
TCCAATTCTACTCTTCTAAG+AGG 0.448706 2_1:+55624944 None:intergenic
TTCTCTATTATTACAGTTGG+AGG 0.450730 2_1:-55624502 Msa0213070:three_prime_UTR
TAGCAACTCTATGGAGTCAT+TGG 0.453627 2_1:-55626640 Msa0213070:CDS
TCTCTATGTGATGAAGAATT+CGG 0.459964 2_1:-55625310 Msa0213070:three_prime_UTR
AGACAAATTGTCTTCTTCAC+TGG 0.467398 2_1:-55624655 Msa0213070:three_prime_UTR
ATGGCGGAGACGGCGAGGTA+CGG 0.471966 2_1:+55628376 Msa0213080:intron
GCTTTGGCAATCACCAATCC+TGG 0.472204 2_1:-55625937 Msa0213070:three_prime_UTR
AATCGAGGTATTTAACTAAG+TGG 0.480482 2_1:-55624255 Msa0213070:three_prime_UTR
TAGCCATAACCTGCAATTGT+TGG 0.488861 2_1:-55626615 Msa0213070:three_prime_UTR
AAGTTCCAAGCACAAGAAGA+AGG 0.489149 2_1:+55624367 None:intergenic
AATCTTGGTGGAGCTCGAAT+AGG 0.490823 2_1:-55625438 Msa0213070:three_prime_UTR
AAGAAGAAGGTTTGAAAGGA+TGG 0.493952 2_1:+55624380 None:intergenic
CTTTGGATTAGGTGAAGATC+CGG 0.494848 2_1:-55624760 Msa0213070:intron
AGGTACCAAGATAATGTCAA+TGG 0.498319 2_1:+55625190 None:intergenic
ATATATCAGACATGCCCTTA+GGG 0.499828 2_1:-55626982 Msa0213070:intron
TTCCAAACACCTACCAGTCC+AGG 0.501998 2_1:+55625919 None:intergenic
ATGTGGTTCTTGCAGGGGAG+CGG 0.504014 2_1:-55625959 Msa0213070:intron
ACTCCTACCACAACAACATC+AGG 0.506236 2_1:+55626315 None:intergenic
TTGCTGGAATACTCTTGACT+TGG 0.507603 2_1:-55625386 Msa0213070:three_prime_UTR
TGCAATGCCACCACAAAGTA+GGG 0.510435 2_1:+55624344 None:intergenic
TGCCCTTAGGGATGGATGGA+TGG 0.515123 2_1:-55626970 Msa0213070:CDS
TGATGTTGATAATGAATTTG+AGG 0.521778 2_1:-55626727 Msa0213070:CDS
TTGCAATGCCACCACAAAGT+AGG 0.522397 2_1:+55624343 None:intergenic
TGATAATGTTCTTCAGAGCT+GGG 0.529587 2_1:+55628352 Msa0213080:CDS
TGTGACCAATGCCTAGGTGC+AGG 0.530298 2_1:+55626136 None:intergenic
AACACCTACCAGTCCAGGAT+TGG 0.539428 2_1:+55625924 None:intergenic
ATCAGACATGCCCTTAGGGA+TGG 0.541039 2_1:-55626978 Msa0213070:intron
TTCAAAATGAAACTTACCTA+AGG 0.542574 2_1:-55625088 Msa0213070:three_prime_UTR
GGAACTTCCCTACTTTGTGG+TGG 0.543624 2_1:-55624351 Msa0213070:three_prime_UTR
AAGCCATCCATCCATCCCTA+AGG 0.544993 2_1:+55626967 None:intergenic
GACATGCCCTTAGGGATGGA+TGG 0.546461 2_1:-55626974 Msa0213070:CDS
CCAATGCCTAGGTGCAGGTA+CGG 0.548490 2_1:+55626141 None:intergenic
ATGTTCTTCAGAGCTGGGTA+TGG 0.549422 2_1:+55628357 Msa0213080:intron
AGAGCTGGGTATGGCGGAGA+CGG 0.551603 2_1:+55628366 Msa0213080:intron
GGCAATCACCAATCCTGGAC+TGG 0.554841 2_1:-55625932 Msa0213070:intron
TCTGCATAGCTGAGTGCAAG+TGG 0.559278 2_1:+55625342 None:intergenic
CTCTTGACTTGGTTTAGTGG+AGG 0.561698 2_1:-55625375 Msa0213070:three_prime_UTR
CCAGCAACAAATTCAGCAGA+CGG 0.563226 2_1:+55625402 None:intergenic
TCTCTATTATTACAGTTGGA+GGG 0.566642 2_1:-55624501 Msa0213070:three_prime_UTR
GCACAAGAAGAAGGTTTGAA+AGG 0.568007 2_1:+55624376 None:intergenic
GAAATCCAGGGAAATCTTGG+TGG 0.568219 2_1:-55625450 Msa0213070:intron
CTCAAGACCTGATGTTGTTG+TGG 0.574621 2_1:-55626322 Msa0213070:intron
TTCTTCAGAGCTGGGTATGG+CGG 0.576008 2_1:+55628360 Msa0213080:intron
CCGTACCTGCACCTAGGCAT+TGG 0.578210 2_1:-55626141 Msa0213070:three_prime_UTR
TGTGGTGGCATTGCAAACCT+TGG 0.582228 2_1:-55624336 Msa0213070:three_prime_UTR
CATAACCTGCAATTGTTGGA+AGG 0.584273 2_1:-55626611 Msa0213070:intron
CTGAAGAACATTATCAGGAT+CGG 0.593763 2_1:-55628345 Msa0213070:intron
GGGCTTCTTAATGCAACATG+TGG 0.594840 2_1:-55624481 Msa0213070:three_prime_UTR
ATATTGAGTACTGAAGATCA+TGG 0.599036 2_1:-55625038 Msa0213070:three_prime_UTR
CTAGTGTACAAAATCAGTGC+CGG 0.599653 2_1:+55624741 None:intergenic
ACATTAAACAGACAAAAGAA+AGG 0.602949 2_1:+55625249 None:intergenic
AATTGGACATTCACAAAATG+GGG 0.610443 2_1:-55624928 Msa0213070:three_prime_UTR
AGCCATCCATCCATCCCTAA+GGG 0.619823 2_1:+55626968 None:intergenic
GGCTTCTTAATGCAACATGT+GGG 0.624011 2_1:-55624480 Msa0213070:three_prime_UTR
TGGGTATGGCGGAGACGGCG+AGG 0.654911 2_1:+55628371 Msa0213080:intron
GCGGAGACGGCGAGGTACGG+CGG 0.662251 2_1:+55628379 Msa0213080:intron
TGGCATTGCAAACCTTGGAG+TGG 0.662345 2_1:-55624331 Msa0213070:three_prime_UTR
GTAACACCGTACCTGCACCT+AGG 0.692657 2_1:-55626147 Msa0213070:three_prime_UTR
TGATGTCAATGAGTTATTTG+GGG 0.714769 2_1:-55626751 Msa0213070:CDS
CGGCGGTGAGAAGAAGATCG+CGG 0.716171 2_1:+55628396 Msa0213080:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGAAAATTTCATATAAA+AGG + chr2_1:55627538-55627557 None:intergenic 10.0%
!!! AAAATTGTTTTAGTGATTTT+AGG - chr2_1:55624616-55624635 Msa0213070:three_prime_UTR 15.0%
!!! AAATTGTTTTAGTGATTTTA+GGG - chr2_1:55624617-55624636 Msa0213070:three_prime_UTR 15.0%
!! TGTAAATAACAAATAAGTAA+TGG + chr2_1:55625118-55625137 None:intergenic 15.0%
!!! CATCTAAATTTTCAATAAAA+GGG + chr2_1:55625145-55625164 None:intergenic 15.0%
!! ATATATATACAGCAAATATA+TGG + chr2_1:55625709-55625728 None:intergenic 15.0%
!!! TAGTAATATCATTTTCAAAT+TGG - chr2_1:55626194-55626213 Msa0213070:three_prime_UTR 15.0%
!!! ATTATCTTTGTTATATTGAT+TGG - chr2_1:55626837-55626856 Msa0213070:intron 15.0%
!!! TTATCTTTGTTATATTGATT+GGG - chr2_1:55626838-55626857 Msa0213070:intron 15.0%
!! TTTCAGAAAATTATGTGTTT+CGG - chr2_1:55624567-55624586 Msa0213070:three_prime_UTR 20.0%
!!! AAATCACTAAAACAATTTTC+TGG + chr2_1:55624615-55624634 None:intergenic 20.0%
!!! TGTATCTTTGATTTTAATTG+AGG - chr2_1:55624862-55624881 Msa0213070:intron 20.0%
!!! GTATCTTTGATTTTAATTGA+GGG - chr2_1:55624863-55624882 Msa0213070:intron 20.0%
!!! AAATTTTTTCACTTCTTTTG+TGG + chr2_1:55624956-55624975 None:intergenic 20.0%
!!! TTAGTCTGAGATAAAAATAA+TGG - chr2_1:55624997-55625016 Msa0213070:three_prime_UTR 20.0%
!!! TAGTCTGAGATAAAAATAAT+GGG - chr2_1:55624998-55625017 Msa0213070:three_prime_UTR 20.0%
!!! GAAGTATCTTTTGATTTTTT+TGG - chr2_1:55625025-55625044 Msa0213070:three_prime_UTR 20.0%
!!! CCATCTAAATTTTCAATAAA+AGG + chr2_1:55625146-55625165 None:intergenic 20.0%
!!! CCTTTTATTGAAAATTTAGA+TGG - chr2_1:55625143-55625162 Msa0213070:three_prime_UTR 20.0%
!! ATAAAACCCTAAATAAAAGA+GGG + chr2_1:55625444-55625463 None:intergenic 20.0%
!!! TTGAAGTTTAAAATGCTTTT+CGG - chr2_1:55626033-55626052 Msa0213070:intron 20.0%
!!! AATATCATTTTCAAATTGGT+TGG - chr2_1:55626198-55626217 Msa0213070:three_prime_UTR 20.0%
!!! AGAATTAGTTTCAAATTGTT+GGG - chr2_1:55626425-55626444 Msa0213070:intron 20.0%
!!! TGTTTCATAATTTCATTTTC+AGG - chr2_1:55626481-55626500 Msa0213070:intron 20.0%
!!! ATAATTTCATTTTCAGGTTT+GGG - chr2_1:55626487-55626506 Msa0213070:intron 20.0%
!! AGGTTTATCTTATATATGTA+TGG - chr2_1:55626723-55626742 Msa0213070:CDS 20.0%
!!! TATCTTTGTTATATTGATTG+GGG - chr2_1:55626839-55626858 Msa0213070:intron 20.0%
!!! ATTTGTTTGGTGTTTTATTT+TGG - chr2_1:55626962-55626981 Msa0213070:CDS 20.0%
!! ATCAATCTGCTAAAAATTTA+AGG - chr2_1:55627125-55627144 Msa0213070:intron 20.0%
!!! ACATTTTCAGCTTTTAAAAA+TGG - chr2_1:55627856-55627875 Msa0213070:intron 20.0%
!!! TTTAAAATGGAAGTGTTAAT+TGG + chr2_1:55628432-55628451 Msa0213080:intron 20.0%
!! TTAAATACCTCGATTTAAAA+TGG + chr2_1:55628445-55628464 Msa0213080:intron 20.0%
! TTGCATGTGTAAATTGAATT+AGG - chr2_1:55624425-55624444 Msa0213070:three_prime_UTR 25.0%
! TGCATGTGTAAATTGAATTA+GGG - chr2_1:55624426-55624445 Msa0213070:three_prime_UTR 25.0%
! TGTAAATTGAATTAGGGTTA+GGG - chr2_1:55624432-55624451 Msa0213070:three_prime_UTR 25.0%
!!! GGGTTTTTTGATTTTAATTG+AGG - chr2_1:55624480-55624499 Msa0213070:three_prime_UTR 25.0%
!!! GGTTTGTTTTAGTGATTTTA+GGG - chr2_1:55624671-55624690 Msa0213070:three_prime_UTR 25.0%
! AACAGTGAAACACAAAATTA+AGG + chr2_1:55624732-55624751 None:intergenic 25.0%
!!! TTTGCATTGATTAGTTGATT+TGG - chr2_1:55624754-55624773 Msa0213070:intron 25.0%
! ATTTGAGAGAGAAGAAATAA+TGG - chr2_1:55624972-55624991 Msa0213070:three_prime_UTR 25.0%
! TTTGAGAGAGAAGAAATAAT+GGG - chr2_1:55624973-55624992 Msa0213070:three_prime_UTR 25.0%
! TTGAAAATTTAGATGGACAA+TGG - chr2_1:55625150-55625169 Msa0213070:three_prime_UTR 25.0%
! TAGGAACTGAAAACTAAATT+TGG - chr2_1:55625343-55625362 Msa0213070:three_prime_UTR 25.0%
! CATAAAACCCTAAATAAAAG+AGG + chr2_1:55625445-55625464 None:intergenic 25.0%
!!! CTCTTTTATTTAGGGTTTTA+TGG - chr2_1:55625443-55625462 Msa0213070:three_prime_UTR 25.0%
! CACTGAAATAAATTGTATCA+CGG - chr2_1:55625533-55625552 Msa0213070:intron 25.0%
!! TCAGCTTTTATATATAACGA+TGG + chr2_1:55625845-55625864 None:intergenic 25.0%
! TCATTAGTGAACACTATAAT+TGG - chr2_1:55625865-55625884 Msa0213070:intron 25.0%
! AATGATGTCAATGAGTTATT+TGG - chr2_1:55625952-55625971 Msa0213070:intron 25.0%
! ATGATGTCAATGAGTTATTT+GGG - chr2_1:55625953-55625972 Msa0213070:intron 25.0%
!!! TGATGTTGATAATGAATTTG+AGG - chr2_1:55625978-55625997 Msa0213070:intron 25.0%
! ATTACTACTTCTGATAAAAG+TGG + chr2_1:55626181-55626200 None:intergenic 25.0%
!! CAGAATTAGTTTCAAATTGT+TGG - chr2_1:55626424-55626443 Msa0213070:intron 25.0%
! ATGAAACATCATTTGCATAA+CGG + chr2_1:55626469-55626488 None:intergenic 25.0%
!!! CATAATTTCATTTTCAGGTT+TGG - chr2_1:55626486-55626505 Msa0213070:intron 25.0%
!!! ACAAAAGAGAAATTTTTTGC+AGG - chr2_1:55626589-55626608 Msa0213070:intron 25.0%
! CCTATAATAACTATCAAGTT+AGG + chr2_1:55626626-55626645 None:intergenic 25.0%
!! CCTAACTTGATAGTTATTAT+AGG - chr2_1:55626623-55626642 Msa0213070:exon 25.0%
! TGAAATTGATGAAGTCAATA+AGG - chr2_1:55626703-55626722 Msa0213070:CDS 25.0%
! ATCTTATATATGTATGGATG+TGG - chr2_1:55626729-55626748 Msa0213070:CDS 25.0%
! AATAAAAGAGAAACAACTCA+AGG + chr2_1:55626820-55626839 None:intergenic 25.0%
!!! TAATGCACAAGATATTTTTG+TGG - chr2_1:55626997-55627016 Msa0213070:intron 25.0%
!! TAGCAATTTTTCTCTGAATT+TGG - chr2_1:55627033-55627052 Msa0213070:intron 25.0%
! TCGTTTAGTCAAATTATGTA+TGG - chr2_1:55627228-55627247 Msa0213070:intron 25.0%
! ACATTAAACAGACAAAAGAA+AGG + chr2_1:55627459-55627478 None:intergenic 25.0%
!! ATTTTCCATTGACATTATCT+TGG - chr2_1:55627510-55627529 Msa0213070:intron 25.0%
!! ATCATATATACTTTTCACGA+AGG + chr2_1:55627587-55627606 None:intergenic 25.0%
! TTCAAAATGAAACTTACCTA+AGG - chr2_1:55627617-55627636 Msa0213070:intron 25.0%
! TACCTAAGGAAAAAAAAAGT+TGG - chr2_1:55627631-55627650 Msa0213070:intron 25.0%
!!! CATTTGTTTTTCATATCCTT+TGG - chr2_1:55627928-55627947 Msa0213070:intron 25.0%
!! TTTTTCATATCCTTTGGATT+AGG - chr2_1:55627934-55627953 Msa0213070:intron 25.0%
!! GAACAATTTTATATACCTGT+AGG + chr2_1:55628077-55628096 None:intergenic 25.0%
! GTATTCTCTATTATTACAGT+TGG - chr2_1:55628200-55628219 Msa0213070:intron 25.0%
! GAATACTTACAACAACAATT+TGG + chr2_1:55628294-55628313 Msa0213080:CDS 25.0%
!! TGTTGTAAGTATTCTCTTTT+AGG - chr2_1:55628300-55628319 Msa0213070:intron 25.0%
!!! GAAGTGTTAATTGGATTTTA+GGG + chr2_1:55628423-55628442 Msa0213080:intron 25.0%
TTTACACATGCAAATTCTGA+AGG + chr2_1:55624418-55624437 None:intergenic 30.0%
GTGTAAATTGAATTAGGGTT+AGG - chr2_1:55624431-55624450 Msa0213070:three_prime_UTR 30.0%
!!! TAATTGAGGTTTTGATTCCT+TGG - chr2_1:55624494-55624513 Msa0213070:three_prime_UTR 30.0%
TAAACAACAACACAAAACCA+AGG + chr2_1:55624514-55624533 None:intergenic 30.0%
!!! GATTTTAGGGTTTCATTTCT+TGG - chr2_1:55624630-55624649 Msa0213070:three_prime_UTR 30.0%
!!! TTGGTTTTGGGTTTGATTTT+AGG - chr2_1:55624649-55624668 Msa0213070:three_prime_UTR 30.0%
!!! TGGTTTTGGGTTTGATTTTA+GGG - chr2_1:55624650-55624669 Msa0213070:three_prime_UTR 30.0%
!!! GGGTTTGTTTTAGTGATTTT+AGG - chr2_1:55624670-55624689 Msa0213070:three_prime_UTR 30.0%
!! ATTAGTTGATTTGGAATGAC+AGG - chr2_1:55624763-55624782 Msa0213070:intron 30.0%
!! TTAGTTGATTTGGAATGACA+GGG - chr2_1:55624764-55624783 Msa0213070:intron 30.0%
!!! TTTGATTTTAATTGAGGGTC+TGG - chr2_1:55624868-55624887 Msa0213070:intron 30.0%
ACTTTCACAGTGATTTAGTT+AGG - chr2_1:55625324-55625343 Msa0213070:three_prime_UTR 30.0%
AAGAATAGTGAGAGTAGAAT+TGG - chr2_1:55625477-55625496 Msa0213070:intron 30.0%
TGAAATAAATTGTATCACGG+AGG - chr2_1:55625536-55625555 Msa0213070:intron 30.0%
AAAAACATACCTGAATTCGA+CGG + chr2_1:55625800-55625819 None:intergenic 30.0%
!!! CAGGTATGTTTTTAGTCTAT+AGG - chr2_1:55625807-55625826 Msa0213070:intron 30.0%
!!! TTTTTTAGGCGTCGAAATTA+AGG - chr2_1:55625927-55625946 Msa0213070:intron 30.0%
TGATGTCAATGAGTTATTTG+GGG - chr2_1:55625954-55625973 Msa0213070:intron 30.0%
!!! TTCATTGCATCTTTTTTTGC+AGG - chr2_1:55626293-55626312 Msa0213070:intron 30.0%
AATGATGTAAACATGTCTGA+TGG - chr2_1:55626648-55626667 Msa0213070:CDS 30.0%
! TGTTATATTGATTGGGGATA+TGG - chr2_1:55626845-55626864 Msa0213070:intron 30.0%
TGTGTCTTGAATTTATGTTG+TGG - chr2_1:55626886-55626905 Msa0213070:intron 30.0%
AGGAAAGCAGTTATCAAAAA+AGG + chr2_1:55626913-55626932 None:intergenic 30.0%
!! TTGATAACTGCTTTCCTTTT+TGG - chr2_1:55626916-55626935 Msa0213070:intron 30.0%
AAAATTGCTATATCTAGCCA+AGG + chr2_1:55627023-55627042 None:intergenic 30.0%
ATTGAGGAGAATAAAAGTTG+AGG - chr2_1:55627089-55627108 Msa0213070:intron 30.0%
TTGAATACTTAGTCTATTGC+TGG - chr2_1:55627152-55627171 Msa0213070:intron 30.0%
TGAATACTTAGTCTATTGCT+GGG - chr2_1:55627153-55627172 Msa0213070:intron 30.0%
TCTCTATGTGATGAAGAATT+CGG - chr2_1:55627395-55627414 Msa0213070:intron 30.0%
!! TTTAGCTTGTGAAAGAATTC+AGG - chr2_1:55627478-55627497 Msa0213070:intron 30.0%
!!! CACCAACTTTTTTTTTCCTT+AGG + chr2_1:55627636-55627655 None:intergenic 30.0%
!! ATATTGAGTACTGAAGATCA+TGG - chr2_1:55627667-55627686 Msa0213070:intron 30.0%
!! AAACTGAGAAGAGGTTTTAT+TGG + chr2_1:55627713-55627732 None:intergenic 30.0%
AATATGGAAAAACTGAGAAG+AGG + chr2_1:55627722-55627741 None:intergenic 30.0%
TATTAGGAACAGACAGAATA+TGG + chr2_1:55627738-55627757 None:intergenic 30.0%
! CTCTTCTAAGAGGAATTATT+AGG + chr2_1:55627754-55627773 None:intergenic 30.0%
AGAATTGGACATTCACAAAA+TGG - chr2_1:55627775-55627794 Msa0213070:intron 30.0%
GAATTGGACATTCACAAAAT+GGG - chr2_1:55627776-55627795 Msa0213070:intron 30.0%
AATTGGACATTCACAAAATG+GGG - chr2_1:55627777-55627796 Msa0213070:intron 30.0%
! TTGATCTTTCGACATTTTTC+AGG + chr2_1:55627825-55627844 None:intergenic 30.0%
TTCTCTATTATTACAGTTGG+AGG - chr2_1:55628203-55628222 Msa0213070:intron 30.0%
TCTCTATTATTACAGTTGGA+GGG - chr2_1:55628204-55628223 Msa0213070:intron 30.0%
!!! GGAAGTGTTAATTGGATTTT+AGG + chr2_1:55628424-55628443 Msa0213080:intron 30.0%
! AACACTTCCATTTTAAATCG+AGG - chr2_1:55628435-55628454 Msa0213070:five_prime_UTR 30.0%
! AATCGAGGTATTTAACTAAG+TGG - chr2_1:55628450-55628469 Msa0213070:five_prime_UTR 30.0%
! TGATAATGTTCTTCAGAGCT+GGG + chr2_1:55624356-55624375 None:intergenic 35.0%
CTGAAGAACATTATCAGGAT+CGG - chr2_1:55624360-55624379 Msa0213070:three_prime_UTR 35.0%
! TGCTTGCATGTTTTCAGAAA+AGG - chr2_1:55624459-55624478 Msa0213070:three_prime_UTR 35.0%
! GCTTGCATGTTTTCAGAAAA+GGG - chr2_1:55624460-55624479 Msa0213070:three_prime_UTR 35.0%
! AGGGTTTCATTTCTTGGTTT+TGG - chr2_1:55624636-55624655 Msa0213070:three_prime_UTR 35.0%
!! GGGTTTCATTTCTTGGTTTT+GGG - chr2_1:55624637-55624656 Msa0213070:three_prime_UTR 35.0%
!! TAGTTGATTTGGAATGACAG+GGG - chr2_1:55624765-55624784 Msa0213070:intron 35.0%
TTTGACGATGCTAATGTTTG+TGG - chr2_1:55624808-55624827 Msa0213070:intron 35.0%
! TTAGATGGACAATGGATTTG+AGG - chr2_1:55625158-55625177 Msa0213070:three_prime_UTR 35.0%
! ACAAGCAGCTTTTGATATGA+TGG - chr2_1:55625185-55625204 Msa0213070:three_prime_UTR 35.0%
CAGTCAACAGAGGAAAAAAA+GGG - chr2_1:55625212-55625231 Msa0213070:three_prime_UTR 35.0%
ATGCTTCTGATCCTTTGATT+TGG - chr2_1:55625404-55625423 Msa0213070:three_prime_UTR 35.0%
! TGCTTCTGATCCTTTGATTT+GGG - chr2_1:55625405-55625424 Msa0213070:three_prime_UTR 35.0%
AAATAAAAGAGGGCTCATCA+AGG + chr2_1:55625434-55625453 None:intergenic 35.0%
!! TGATGAGCCCTCTTTTATTT+AGG - chr2_1:55625434-55625453 Msa0213070:three_prime_UTR 35.0%
!! GATGAGCCCTCTTTTATTTA+GGG - chr2_1:55625435-55625454 Msa0213070:three_prime_UTR 35.0%
CGGAGGAAACAATCAATATT+AGG - chr2_1:55625553-55625572 Msa0213070:intron 35.0%
TATATATCAGACATGCCCTT+AGG - chr2_1:55625722-55625741 Msa0213070:intron 35.0%
ATATATCAGACATGCCCTTA+GGG - chr2_1:55625723-55625742 Msa0213070:intron 35.0%
TAATGATGCTAGCAACTCTA+TGG - chr2_1:55626056-55626075 Msa0213070:intron 35.0%
! CGTTGTCTAATTTTGCGAAT+TGG + chr2_1:55626330-55626349 None:intergenic 35.0%
TTCAAAAACACCAGCAACTT+AGG + chr2_1:55626530-55626549 None:intergenic 35.0%
CAAGAAACCTCATTCCAAAA+AGG + chr2_1:55626933-55626952 None:intergenic 35.0%
!!! CTTTTTGGAATGAGGTTTCT+TGG - chr2_1:55626931-55626950 Msa0213070:CDS 35.0%
!!! TGGTGTTTTATTTTGGAGTG+AGG - chr2_1:55626969-55626988 Msa0213070:CDS 35.0%
!! ACAAGATATTTTTGTGGCCT+TGG - chr2_1:55627003-55627022 Msa0213070:intron 35.0%
! GAATTTGGTTCATTTAACCG+AGG - chr2_1:55627048-55627067 Msa0213070:intron 35.0%
AGTCGAAACACAATGTATTG+AGG - chr2_1:55627073-55627092 Msa0213070:intron 35.0%
! TATGTATGGCATTGAAATCC+AGG - chr2_1:55627242-55627261 Msa0213070:intron 35.0%
! ATGTATGGCATTGAAATCCA+GGG - chr2_1:55627243-55627262 Msa0213070:intron 35.0%
ATTGAAATCCAGGGAAATCT+TGG - chr2_1:55627252-55627271 Msa0213070:intron 35.0%
!! ATACTCTTGACTTGGTTTAG+TGG - chr2_1:55627327-55627346 Msa0213070:intron 35.0%
AGGTACCAAGATAATGTCAA+TGG + chr2_1:55627518-55627537 None:intergenic 35.0%
TCCTCTTAGAAGAGTAGAAT+TGG - chr2_1:55627760-55627779 Msa0213070:intron 35.0%
TCCAATTCTACTCTTCTAAG+AGG + chr2_1:55627764-55627783 None:intergenic 35.0%
ATTGCCTTGGAAAACTGATA+CGG + chr2_1:55627898-55627917 None:intergenic 35.0%
ATGATGAGAAAGAATTGCCT+TGG + chr2_1:55627911-55627930 None:intergenic 35.0%
AGACAAATTGTCTTCTTCAC+TGG - chr2_1:55628050-55628069 Msa0213070:intron 35.0%
AATGCAACATGTGGGAATAT+TGG - chr2_1:55628233-55628252 Msa0213070:intron 35.0%
AAGAAGAAGGTTTGAAAGGA+TGG + chr2_1:55628328-55628347 Msa0213080:CDS 35.0%
! CTGATAATGTTCTTCAGAGC+TGG + chr2_1:55624357-55624376 None:intergenic 40.0%
!! CAGCTCTGAAGAACATTATC+AGG - chr2_1:55624355-55624374 Msa0213070:three_prime_UTR 40.0%
!!! AGGGTCTGGTCTTTTGATTT+TGG - chr2_1:55624882-55624901 Msa0213070:intron 40.0%
!!! GGGTCTGGTCTTTTGATTTT+GGG - chr2_1:55624883-55624902 Msa0213070:three_prime_UTR 40.0%
! ATGGACAATGGATTTGAGGA+TGG - chr2_1:55625162-55625181 Msa0213070:three_prime_UTR 40.0%
GCAGTCAACAGAGGAAAAAA+AGG - chr2_1:55625211-55625230 Msa0213070:three_prime_UTR 40.0%
AATCACTGTGAAAGTGAGAG+TGG + chr2_1:55625319-55625338 None:intergenic 40.0%
ATCAAGGACTCCCAAATCAA+AGG + chr2_1:55625418-55625437 None:intergenic 40.0%
CGACGGTACATTTGAATCTT+CGG + chr2_1:55625783-55625802 None:intergenic 40.0%
TCAAATGTACCGTCGAATTC+AGG - chr2_1:55625788-55625807 Msa0213070:intron 40.0%
TAGCAACTCTATGGAGTCAT+TGG - chr2_1:55626065-55626084 Msa0213070:intron 40.0%
TAGCCATAACCTGCAATTGT+TGG - chr2_1:55626090-55626109 Msa0213070:intron 40.0%
CTTCCAACAATTGCAGGTTA+TGG + chr2_1:55626096-55626115 None:intergenic 40.0%
CATAACCTGCAATTGTTGGA+AGG - chr2_1:55626094-55626113 Msa0213070:intron 40.0%
TCATACCTTCCAACAATTGC+AGG + chr2_1:55626102-55626121 None:intergenic 40.0%
! GCAATTGTTGGAAGGTATGA+TGG - chr2_1:55626102-55626121 Msa0213070:intron 40.0%
ATTAGTACATCACACACGCA+AGG + chr2_1:55626679-55626698 None:intergenic 40.0%
TTGCGTGTGTGATGTACTAA+TGG - chr2_1:55626678-55626697 Msa0213070:CDS 40.0%
TGCGTGTGTGATGTACTAAT+GGG - chr2_1:55626679-55626698 Msa0213070:CDS 40.0%
ACTGGTAGGTGTTTGGAAAT+GGG - chr2_1:55626791-55626810 Msa0213070:intron 40.0%
!!! CTGCTTTCCTTTTTGGAATG+AGG - chr2_1:55626923-55626942 Msa0213070:CDS 40.0%
! CTTGGTGCACAAGATTTGTT+TGG - chr2_1:55626949-55626968 Msa0213070:CDS 40.0%
TTGCTGGAATACTCTTGACT+TGG - chr2_1:55627319-55627338 Msa0213070:intron 40.0%
! ATCTCCGTATCAGTTTTCCA+AGG - chr2_1:55627891-55627910 Msa0213070:intron 40.0%
! CTTTGGATTAGGTGAAGATC+CGG - chr2_1:55627945-55627964 Msa0213070:intron 40.0%
CTAGTGTACAAAATCAGTGC+CGG + chr2_1:55627967-55627986 None:intergenic 40.0%
!!! GCACTGATTTTGTACACTAG+CGG - chr2_1:55627967-55627986 Msa0213070:intron 40.0%
!!! CACTGATTTTGTACACTAGC+GGG - chr2_1:55627968-55627987 Msa0213070:intron 40.0%
GGCTTCTTAATGCAACATGT+GGG - chr2_1:55628225-55628244 Msa0213070:intron 40.0%
GCACAAGAAGAAGGTTTGAA+AGG + chr2_1:55628332-55628351 Msa0213080:CDS 40.0%
TCAAACCTTCTTCTTGTGCT+TGG - chr2_1:55628333-55628352 Msa0213070:intron 40.0%
AAGTTCCAAGCACAAGAAGA+AGG + chr2_1:55628341-55628360 Msa0213080:CDS 40.0%
!! CATATTTTTGCTCCACTCCA+AGG + chr2_1:55628389-55628408 Msa0213080:intron 40.0%
ATGTTCTTCAGAGCTGGGTA+TGG + chr2_1:55624351-55624370 None:intergenic 45.0%
GAACATTATCAGGATCGGTG+AGG - chr2_1:55624365-55624384 Msa0213070:three_prime_UTR 45.0%
TGTGAAAGTGAGAGTGGTGA+TGG + chr2_1:55625313-55625332 None:intergenic 45.0%
CTCAAGACCTGATGTTGTTG+TGG - chr2_1:55626383-55626402 Msa0213070:three_prime_UTR 45.0%
AGACCTGATGTTGTTGTGGT+AGG - chr2_1:55626387-55626406 Msa0213070:three_prime_UTR 45.0%
ACTCCTACCACAACAACATC+AGG + chr2_1:55626393-55626412 None:intergenic 45.0%
! TTGGGTATCCGCTTCTTTTG+AGG - chr2_1:55626443-55626462 Msa0213070:intron 45.0%
CATAACGGCCTCAAAAGAAG+CGG + chr2_1:55626454-55626473 None:intergenic 45.0%
! CTCTTTTGTGACCAATGCCT+AGG + chr2_1:55626578-55626597 None:intergenic 45.0%
GCGTGTGTGATGTACTAATG+GGG - chr2_1:55626680-55626699 Msa0213070:CDS 45.0%
TGTATGGATGTGGTTCTTGC+AGG - chr2_1:55626739-55626758 Msa0213070:CDS 45.0%
GTATGGATGTGGTTCTTGCA+GGG - chr2_1:55626740-55626759 Msa0213070:CDS 45.0%
TATGGATGTGGTTCTTGCAG+GGG - chr2_1:55626741-55626760 Msa0213070:CDS 45.0%
GACTGGTAGGTGTTTGGAAA+TGG - chr2_1:55626790-55626809 Msa0213070:intron 45.0%
CATTGTGTTTCGACTGTCCT+CGG + chr2_1:55627068-55627087 None:intergenic 45.0%
GAAATCCAGGGAAATCTTGG+TGG - chr2_1:55627255-55627274 Msa0213070:intron 45.0%
AATCTTGGTGGAGCTCGAAT+AGG - chr2_1:55627267-55627286 Msa0213070:intron 45.0%
CCAGCAACAAATTCAGCAGA+CGG + chr2_1:55627306-55627325 None:intergenic 45.0%
!! CTCTTGACTTGGTTTAGTGG+AGG - chr2_1:55627330-55627349 Msa0213070:intron 45.0%
CGGATCTTCACCTAATCCAA+AGG + chr2_1:55627947-55627966 None:intergenic 45.0%
TTGTACACTAGCGGGACAAT+AGG - chr2_1:55627976-55627995 Msa0213070:intron 45.0%
GGGCTTCTTAATGCAACATG+TGG - chr2_1:55628224-55628243 Msa0213070:intron 45.0%
CTTGGAACTTCCCTACTTTG+TGG - chr2_1:55628351-55628370 Msa0213070:CDS 45.0%
TGCAATGCCACCACAAAGTA+GGG + chr2_1:55628364-55628383 Msa0213080:intron 45.0%
TTGCAATGCCACCACAAAGT+AGG + chr2_1:55628365-55628384 Msa0213080:intron 45.0%
!!! TAATTAGATATTATTTTTTT+AGG - chr2_1:55625913-55625932 Msa0213070:intron 5.0%
TTCTTCAGAGCTGGGTATGG+CGG + chr2_1:55624348-55624367 None:intergenic 50.0%
TGATGGCAAGCAGTCAACAG+AGG - chr2_1:55625202-55625221 Msa0213070:three_prime_UTR 50.0%
!! GAAAGTGAGAGTGGTGATGG+AGG + chr2_1:55625310-55625329 None:intergenic 50.0%
ATCAGACATGCCCTTAGGGA+TGG - chr2_1:55625727-55625746 Msa0213070:intron 50.0%
AGCCATCCATCCATCCCTAA+GGG + chr2_1:55625740-55625759 None:intergenic 50.0%
AAGCCATCCATCCATCCCTA+AGG + chr2_1:55625741-55625760 None:intergenic 50.0%
! TTTGAGGACACTGCTGCTTC+AGG - chr2_1:55625994-55626013 Msa0213070:intron 50.0%
TGCAGCAGATCCTAAGTTGC+TGG - chr2_1:55626517-55626536 Msa0213070:intron 50.0%
GCTTTGGCAATCACCAATCC+TGG - chr2_1:55626768-55626787 Msa0213070:CDS 50.0%
!! ATCACCAATCCTGGACTGGT+AGG - chr2_1:55626777-55626796 Msa0213070:intron 50.0%
AACACCTACCAGTCCAGGAT+TGG + chr2_1:55626784-55626803 None:intergenic 50.0%
!! ATCCTGGACTGGTAGGTGTT+TGG - chr2_1:55626784-55626803 Msa0213070:intron 50.0%
TTCCAAACACCTACCAGTCC+AGG + chr2_1:55626789-55626808 None:intergenic 50.0%
!! CCGTCTGCTGAATTTGTTGC+TGG - chr2_1:55627303-55627322 Msa0213070:intron 50.0%
TCTGCATAGCTGAGTGCAAG+TGG + chr2_1:55627366-55627385 None:intergenic 50.0%
GTCTTCTTCACTGGTCCTAC+AGG - chr2_1:55628059-55628078 Msa0213070:intron 50.0%
GGAACTTCCCTACTTTGTGG+TGG - chr2_1:55628354-55628373 Msa0213070:CDS 50.0%
TGTGGTGGCATTGCAAACCT+TGG - chr2_1:55628369-55628388 Msa0213070:CDS 50.0%
TGGCATTGCAAACCTTGGAG+TGG - chr2_1:55628374-55628393 Msa0213070:CDS 50.0%
GACATGCCCTTAGGGATGGA+TGG - chr2_1:55625731-55625750 Msa0213070:intron 55.0%
TGCCCTTAGGGATGGATGGA+TGG - chr2_1:55625735-55625754 Msa0213070:intron 55.0%
GTAACACCGTACCTGCACCT+AGG - chr2_1:55626558-55626577 Msa0213070:intron 55.0%
CCAATGCCTAGGTGCAGGTA+CGG + chr2_1:55626567-55626586 None:intergenic 55.0%
TGTGACCAATGCCTAGGTGC+AGG + chr2_1:55626572-55626591 None:intergenic 55.0%
ATGTGGTTCTTGCAGGGGAG+CGG - chr2_1:55626746-55626765 Msa0213070:CDS 55.0%
GGCAATCACCAATCCTGGAC+TGG - chr2_1:55626773-55626792 Msa0213070:CDS 55.0%
GAGCTCCACCAAGATTTCCC+TGG + chr2_1:55627263-55627282 None:intergenic 55.0%
CGGCGGTGAGAAGAAGATCG+CGG + chr2_1:55624312-55624331 None:intergenic 60.0%
! AGAGCTGGGTATGGCGGAGA+CGG + chr2_1:55624342-55624361 None:intergenic 60.0%
CCGTACCTGCACCTAGGCAT+TGG - chr2_1:55626564-55626583 Msa0213070:intron 60.0%
TTCTTGCAGGGGAGCGGCTT+TGG - chr2_1:55626752-55626771 Msa0213070:CDS 60.0%
! ATGGCGGAGACGGCGAGGTA+CGG + chr2_1:55624332-55624351 None:intergenic 65.0%
! TGGGTATGGCGGAGACGGCG+AGG + chr2_1:55624337-55624356 None:intergenic 70.0%
GCGGAGACGGCGAGGTACGG+CGG + chr2_1:55624329-55624348 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 55624249 55628478 55624249 ID=Msa0213070;Name=Msa0213070
chr2_1 mRNA 55624249 55628478 55624249 ID=Msa0213070-mRNA-1;Parent=Msa0213070;Name=Msa0213070-mRNA-1;_AED=0.17;_eAED=0.17;_QI=45|0.85|1|1|0.85|0.75|8|1359|107
chr2_1 exon 55624249 55624771 55624249 ID=Msa0213070-mRNA-1:exon:15462;Parent=Msa0213070-mRNA-1
chr2_1 exon 55624883 55625463 55624883 ID=Msa0213070-mRNA-1:exon:15461;Parent=Msa0213070-mRNA-1
chr2_1 exon 55625933 55625966 55625933 ID=Msa0213070-mRNA-1:exon:15460;Parent=Msa0213070-mRNA-1
chr2_1 exon 55626117 55626224 55626117 ID=Msa0213070-mRNA-1:exon:15459;Parent=Msa0213070-mRNA-1
chr2_1 exon 55626323 55626412 55626323 ID=Msa0213070-mRNA-1:exon:15458;Parent=Msa0213070-mRNA-1
chr2_1 exon 55626612 55626792 55626612 ID=Msa0213070-mRNA-1:exon:15457;Parent=Msa0213070-mRNA-1
chr2_1 exon 55626918 55626995 55626918 ID=Msa0213070-mRNA-1:exon:15456;Parent=Msa0213070-mRNA-1
chr2_1 exon 55628346 55628478 55628346 ID=Msa0213070-mRNA-1:exon:15455;Parent=Msa0213070-mRNA-1
chr2_1 five_prime_UTR 55628434 55628478 55628434 ID=Msa0213070-mRNA-1:five_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 CDS 55628346 55628433 55628346 ID=Msa0213070-mRNA-1:cds;Parent=Msa0213070-mRNA-1
chr2_1 CDS 55626918 55626995 55626918 ID=Msa0213070-mRNA-1:cds;Parent=Msa0213070-mRNA-1
chr2_1 CDS 55626635 55626792 55626635 ID=Msa0213070-mRNA-1:cds;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55626612 55626634 55626612 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55626323 55626412 55626323 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55626117 55626224 55626117 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55625933 55625966 55625933 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55624883 55625463 55624883 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
chr2_1 three_prime_UTR 55624249 55624771 55624249 ID=Msa0213070-mRNA-1:three_prime_utr;Parent=Msa0213070-mRNA-1
Gene Sequence

>Msa0213070

ATGATACTCTCTCAGCCGCGATCTTCTTCTCACCGCCGTACCTCGCCGTCTCCGCCATACCCAGCTCTGAAGAACATTATCAGGATCGACATGCCCTTAGGGATGGATGGATGGCTTATTTGCTGTATATATAATGCCGAAGATTCAAATGTACCGTCGAATTCAGGCGTCGAAATTAAGGTGAATGATGTCAATGAGTTATTTGGGGCTGATGTTGATAATGAATTTGAGGACACTGCTGCTTCAGGTAAATCTAACAATGAATTGAAGTTTAAAATGCTTTTCGGTAATGATGCTAGCAACTCTATGGAGTCATTGGCATAG
Protein sequence

>Msa0213070

MILSQPRSSSHRRTSPSPPYPALKNIIRIDMPLGMDGWLICCIYNAEDSNVPSNSGVEIKVNDVNELFGADVDNEFEDTAASGKSNNELKFKMLFGNDASNSMESLA*