AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0858770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCCAACAAAATTATTAAT+AGG 0.224730 5_4:-56550367 None:intergenic
ACAAGGTTGCAACGATTTAT+AGG 0.244797 5_4:-56550029 None:intergenic
CGATGCAGTCTAGCAGAAAT+TGG 0.245631 5_4:+56550572 Msa0858770:three_prime_UTR
CATCTTAATCTCATTAGTTT+TGG 0.281168 5_4:+56550421 Msa0858770:three_prime_UTR
CCTTAGAATCTCGTTATATT+CGG 0.330694 5_4:+56550473 Msa0858770:three_prime_UTR
TAGATCTCTGTCTCATCTTT+CGG 0.343914 5_4:-56550270 None:intergenic
CAAGGTTGCAACGATTTATA+GGG 0.368412 5_4:-56550028 None:intergenic
CCGAATATAACGAGATTCTA+AGG 0.379000 5_4:-56550473 None:intergenic
TTTGTCGTAAACCTAGTTGA+TGG 0.405515 5_4:-56550071 None:intergenic
GAGATCTAAGTAGTGAGCTT+TGG 0.432068 5_4:+56550285 Msa0858770:exon
AGTGGAAGAAGAAGCGTATG+AGG 0.483173 5_4:+56550230 Msa0858770:intron
TCTTTCAGCGGCCATCAACT+AGG 0.508309 5_4:+56550060 Msa0858770:CDS
GATGCAGTCTAGCAGAAATT+GGG 0.521054 5_4:+56550573 Msa0858770:three_prime_UTR
ACCTTGGCTCTCATCTTGAA+CGG 0.524585 5_4:-56550102 None:intergenic
AGCGTATGAGGAGATTGAAG+AGG 0.544618 5_4:+56550242 Msa0858770:CDS
ACCGTTCAAGATGAGAGCCA+AGG 0.659345 5_4:+56550101 Msa0858770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTGATTTTATCTTAATTT+TGG + chr5_4:56550174-56550193 Msa0858770:intron 15.0%
!!! AATCCTATTAATAATTTTGT+TGG + chr5_4:56550364-56550383 Msa0858770:three_prime_UTR 15.0%
!! TATCCAACAAAATTATTAAT+AGG - chr5_4:56550370-56550389 None:intergenic 15.0%
!!! AACTACTTTGATAATTTTTT+TGG + chr5_4:56550513-56550532 Msa0858770:three_prime_UTR 15.0%
!!! ACTACTTTGATAATTTTTTT+GGG + chr5_4:56550514-56550533 Msa0858770:three_prime_UTR 15.0%
!!! TTTCATGTTTTGATACATTA+TGG + chr5_4:56550392-56550411 Msa0858770:three_prime_UTR 20.0%
!!! TTCATGTTTTGATACATTAT+GGG + chr5_4:56550393-56550412 Msa0858770:three_prime_UTR 20.0%
!!! CATCTTAATCTCATTAGTTT+TGG + chr5_4:56550421-56550440 Msa0858770:three_prime_UTR 25.0%
!! TTGTCTTTTTCATCTTTCAG+CGG + chr5_4:56550048-56550067 Msa0858770:CDS 30.0%
!! CTATTGTTTGCTTTTATCAG+TGG + chr5_4:56550212-56550231 Msa0858770:intron 30.0%
CCTTAGAATCTCGTTATATT+CGG + chr5_4:56550473-56550492 Msa0858770:three_prime_UTR 30.0%
CAAGGTTGCAACGATTTATA+GGG - chr5_4:56550031-56550050 None:intergenic 35.0%
ACAAGGTTGCAACGATTTAT+AGG - chr5_4:56550032-56550051 None:intergenic 35.0%
GCTGAAAGATGAAAAAGACA+AGG - chr5_4:56550049-56550068 None:intergenic 35.0%
TTTGTCGTAAACCTAGTTGA+TGG - chr5_4:56550074-56550093 None:intergenic 35.0%
AGCAGTGAAATAGTTTACCT+TGG - chr5_4:56550121-56550140 None:intergenic 35.0%
TAGATCTCTGTCTCATCTTT+CGG - chr5_4:56550273-56550292 None:intergenic 35.0%
CCGAATATAACGAGATTCTA+AGG - chr5_4:56550476-56550495 None:intergenic 35.0%
GAGATCTAAGTAGTGAGCTT+TGG + chr5_4:56550285-56550304 Msa0858770:exon 40.0%
GATGCAGTCTAGCAGAAATT+GGG + chr5_4:56550573-56550592 Msa0858770:three_prime_UTR 40.0%
! ACCTTGGCTCTCATCTTGAA+CGG - chr5_4:56550105-56550124 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr5_4:56550230-56550249 Msa0858770:intron 45.0%
AGCGTATGAGGAGATTGAAG+AGG + chr5_4:56550242-56550261 Msa0858770:CDS 45.0%
CGATGCAGTCTAGCAGAAAT+TGG + chr5_4:56550572-56550591 Msa0858770:three_prime_UTR 45.0%
TCTTTCAGCGGCCATCAACT+AGG + chr5_4:56550060-56550079 Msa0858770:CDS 50.0%
ACCGTTCAAGATGAGAGCCA+AGG + chr5_4:56550101-56550120 Msa0858770:CDS 50.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 56550020 56550602 56550020 ID=Msa0858770;Name=Msa0858770
chr5_4 mRNA 56550020 56550602 56550020 ID=Msa0858770-mRNA-1;Parent=Msa0858770;Name=Msa0858770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|309|54
chr5_4 exon 56550020 56550122 56550020 ID=Msa0858770-mRNA-1:exon:17262;Parent=Msa0858770-mRNA-1
chr5_4 exon 56550232 56550602 56550232 ID=Msa0858770-mRNA-1:exon:17263;Parent=Msa0858770-mRNA-1
chr5_4 CDS 56550020 56550122 56550020 ID=Msa0858770-mRNA-1:cds;Parent=Msa0858770-mRNA-1
chr5_4 CDS 56550232 56550293 56550232 ID=Msa0858770-mRNA-1:cds;Parent=Msa0858770-mRNA-1
chr5_4 three_prime_UTR 56550294 56550602 56550294 ID=Msa0858770-mRNA-1:three_prime_utr;Parent=Msa0858770-mRNA-1
Gene Sequence

>Msa0858770

CTTATCTTCCCTATAAATCGTTGCAACCTTGTCTTTTTCATCTTTCAGCGGCCATCAACTAGGTTTACGACAAAGCAAAACACCGTTCAAGATGAGAGCCAAGTGGAAGAAGAAGCGTATGAGGAGATTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA

Protein sequence

>Msa0858770

LIFPINRCNLVFFIFQRPSTRFTTKQNTVQDESQVEEEAYEEIEEEAPKDETEI*