AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0417440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0417440 MtrunA17_Chr3g0114931 93.034 445 23 3 1 443 1 439 0.0 802
Msa0417440 MtrunA17_Chr3g0114921 54.788 449 185 9 1 439 1 441 8.26e-154 443
Msa0417440 MtrunA17_Chr3g0078481 64.470 349 119 4 96 443 36 380 8.51e-145 418
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0417440 AT2G41705.3 51.294 425 192 7 25 440 37 455 9.14e-122 362
Msa0417440 AT2G41705.2 51.294 425 192 7 25 440 37 455 9.14e-122 362
Msa0417440 AT2G41705.4 51.294 425 192 7 25 440 37 455 9.14e-122 362
Msa0417440 AT2G41705.1 51.294 425 192 7 25 440 37 455 9.14e-122 362

Find 114 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAACTGCAAAATCCTTAT+TGG 0.160811 3_2:-64626743 Msa0417440:three_prime_UTR
CCTTGATCTGCCATCAAATA+TGG 0.217091 3_2:-64628885 Msa0417440:intron
AGGACTTCAATTTGGTCTAT+TGG 0.243981 3_2:-64627249 Msa0417440:CDS
TGTCGATCATCATTGGTTTC+TGG 0.244597 3_2:-64627880 Msa0417440:CDS
TACCTATTTGTTTGGGTGTT+TGG 0.268786 3_2:-64627053 Msa0417440:three_prime_UTR
ATTAGGATCTTCGTGGGATT+TGG 0.269040 3_2:-64626936 Msa0417440:three_prime_UTR
CCCATCTTTGTTTCAACAAA+TGG 0.283826 3_2:-64629558 Msa0417440:CDS
TGGTGATATTAGCTTTGTTA+TGG 0.290543 3_2:-64627730 Msa0417440:CDS
GCCTGCATTGTTGGACCTCT+TGG 0.293298 3_2:-64627641 Msa0417440:CDS
GTTGGAAGAGATATTATATT+AGG 0.293744 3_2:-64626953 Msa0417440:three_prime_UTR
AGACTTAACGAGTGCGAAAT+TGG 0.297010 3_2:-64627590 Msa0417440:CDS
CTCAATCACATTCTTGAAAC+AGG 0.298737 3_2:+64627089 None:intergenic
TGTTATTTAATCTAATCTTT+AGG 0.299381 3_2:-64629978 Msa0417440:five_prime_UTR
TCATTTGGACCAAGTGGTTT+TGG 0.299453 3_2:-64628935 Msa0417440:CDS
AACAATAGTCCCACCCAATA+AGG 0.303787 3_2:+64626729 None:intergenic
GGATTATGTACCTATTTGTT+TGG 0.307962 3_2:-64627061 Msa0417440:three_prime_UTR
TGATAGAAAACAAGCATCTT+TGG 0.308824 3_2:-64627181 Msa0417440:CDS
GGACTTCAATTTGGTCTATT+GGG 0.318154 3_2:-64627248 Msa0417440:CDS
ACAATATAGTTATTATTAGA+TGG 0.318561 3_2:+64629600 None:intergenic
TTATATATCAAACCAGAAAA+TGG 0.319807 3_2:-64629663 Msa0417440:CDS
TGAACTGCAAAATCCTTATT+GGG 0.319961 3_2:-64626742 Msa0417440:three_prime_UTR
GTGATAACAGGACTTCAATT+TGG 0.320323 3_2:-64627257 Msa0417440:CDS
ACCAAACCATCCCATCAAAA+AGG 0.332116 3_2:+64628290 None:intergenic
TACTTATTGCAAAAGTCATT+TGG 0.339763 3_2:-64628950 Msa0417440:CDS
GGTGATATTAGCTTTGTTAT+GGG 0.342133 3_2:-64627729 Msa0417440:CDS
GATTATGTACCTATTTGTTT+GGG 0.342788 3_2:-64627060 Msa0417440:three_prime_UTR
GGCAATTCATGTTCCATTGC+AGG 0.342935 3_2:+64629624 None:intergenic
CACTGGATCTTGTCTTTGCT+CGG 0.345396 3_2:-64628138 Msa0417440:CDS
ATCACTATGTTTCTTTCTAT+AGG 0.370649 3_2:+64629925 None:intergenic
TGTTTCGTGTCTAACTCATT+TGG 0.377439 3_2:-64629352 Msa0417440:CDS
TTGTTGGACCTCTTGGTGTA+TGG 0.380313 3_2:-64627634 Msa0417440:CDS
GAATTTGTTGGGTCTGAGTT+AGG 0.382110 3_2:-64626978 Msa0417440:three_prime_UTR
TCCATTTGTTGAAACAAAGA+TGG 0.386494 3_2:+64629557 None:intergenic
TCTTTGCTCGGCTTTGTACT+AGG 0.391256 3_2:-64628126 Msa0417440:intron
GGTCTAACAACAGGCTACTT+AGG 0.394109 3_2:-64628219 Msa0417440:CDS
AATTGTGATACTGTGATAAC+AGG 0.402053 3_2:-64627269 Msa0417440:CDS
TGTTTCTGCTGCTTGTATCA+TGG 0.407601 3_2:-64627522 Msa0417440:CDS
CAAGAATGTGATTGAGCATA+GGG 0.419367 3_2:-64627082 Msa0417440:exon
GACCTCTTGGTGTATGGCTT+AGG 0.423317 3_2:-64627628 Msa0417440:CDS
AGCCAAACACCCAAACAAAT+AGG 0.425540 3_2:+64627051 None:intergenic
TGACAGCTTCCTTCGTCAAT+TGG 0.430603 3_2:-64627771 Msa0417440:CDS
AAAACTTACTGATTTCTTGA+GGG 0.431062 3_2:+64627487 None:intergenic
GTTGGGGTTCTGCAGTTAGC+AGG 0.434224 3_2:-64629812 Msa0417440:CDS
GAAGTTGACTCGACTCAGTC+CGG 0.438547 3_2:+64626494 None:intergenic
CGTGAGTGTGGTTCAGCTGC+AGG 0.439210 3_2:-64629741 Msa0417440:CDS
CATGGAGCGAACGAGCAGTT+GGG 0.449685 3_2:-64629829 Msa0417440:CDS
TGGTTCAGCTGCAGGAGAAA+TGG 0.450536 3_2:-64629733 Msa0417440:CDS
GCCTAACAACTTTCAGCGGC+TGG 0.451556 3_2:-64628194 Msa0417440:CDS
TCCAGCCGCTGAAAGTTGTT+AGG 0.453775 3_2:+64628193 None:intergenic
TGTACAATAGTGAATTTGTT+GGG 0.454314 3_2:-64626989 Msa0417440:three_prime_UTR
CTGGTTTGATATATAAAGAC+AGG 0.458399 3_2:+64629670 None:intergenic
GTAGCATTGTCGATCATCAT+TGG 0.459988 3_2:-64627887 Msa0417440:CDS
TTGTGGTTAGCCTGCATTGT+TGG 0.460887 3_2:-64627650 Msa0417440:CDS
ATGCTCGAGCTCAGTGTCGC+TGG 0.460917 3_2:-64628162 Msa0417440:CDS
CTAGCCATTGGTCTAACAAC+AGG 0.471645 3_2:-64628228 Msa0417440:CDS
CGAAACAAATTCCAGTAGCT+TGG 0.472897 3_2:+64629368 None:intergenic
GTCTAACAACAGGCTACTTA+GGG 0.475229 3_2:-64628218 Msa0417440:CDS
GAGAGGCAAACCATATTTGA+TGG 0.476823 3_2:+64628875 None:intergenic
CTCTTGGTGTATGGCTTAGG+TGG 0.484425 3_2:-64627625 Msa0417440:CDS
AGCTCAGTGTCGCTGGACAC+TGG 0.485522 3_2:-64628155 Msa0417440:CDS
ATGGGGTGTGAGTAGTGCTT+TGG 0.485675 3_2:-64627711 Msa0417440:CDS
CATGAAGTTACCCAAGCTAC+TGG 0.489022 3_2:-64629379 Msa0417440:CDS
TTGTACAATAGTGAATTTGT+TGG 0.491259 3_2:-64626990 Msa0417440:three_prime_UTR
TTGGGTCTGAGTTAGGAAGT+TGG 0.493066 3_2:-64626971 Msa0417440:three_prime_UTR
GAGAGCATTTGTCTATGCTA+TGG 0.494452 3_2:-64627159 Msa0417440:CDS
AAAAGAAAATGAAACGCAAA+TGG 0.498184 3_2:+64627133 None:intergenic
TTGACATTTAAATTTGCTCC+CGG 0.500954 3_2:-64626513 Msa0417440:three_prime_UTR
CCAACTGCTCGTTCGCTCCA+TGG 0.502807 3_2:+64629830 None:intergenic
GAAACAAATTCCAGTAGCTT+GGG 0.506298 3_2:+64629369 None:intergenic
CCATGGAGCGAACGAGCAGT+TGG 0.508369 3_2:-64629830 Msa0417440:CDS
CTTTGTTTCAACAAATGGAA+AGG 0.511711 3_2:-64629553 Msa0417440:CDS
CATTGGTTTCTGGACGGCGA+AGG 0.512919 3_2:-64627870 Msa0417440:CDS
GTGATATTAGCTTTGTTATG+GGG 0.520721 3_2:-64627728 Msa0417440:CDS
CCATATTTGATGGCAGATCA+AGG 0.523172 3_2:+64628885 None:intergenic
AACGAGTGCGAAATTGGAGG+AGG 0.526946 3_2:-64627584 Msa0417440:CDS
GGAGGAAGGAAAAGTGAGAG+TGG 0.527956 3_2:-64629702 Msa0417440:CDS
CCATTTGTTGAAACAAAGAT+GGG 0.530182 3_2:+64629558 None:intergenic
AAGTTGACTCGACTCAGTCC+GGG 0.531437 3_2:+64626495 None:intergenic
GTATCTGAACATCTAGCCAT+TGG 0.532584 3_2:-64628240 Msa0417440:CDS
TAATATCACCAACAGCACTA+TGG 0.535425 3_2:+64627742 None:intergenic
TCAAGAATGTGATTGAGCAT+AGG 0.536915 3_2:-64627083 Msa0417440:exon
ATTGGTTTCTGGACGGCGAA+GGG 0.538719 3_2:-64627869 Msa0417440:CDS
GGACGGCGAAGGGATTCAAA+TGG 0.542568 3_2:-64627859 Msa0417440:CDS
ACCAAGAGGTCCAACAATGC+AGG 0.546644 3_2:+64627640 None:intergenic
CTGGTCACGCCAAAACCACT+TGG 0.550500 3_2:+64628926 None:intergenic
CAAAAGTCATTTGGACCAAG+TGG 0.552223 3_2:-64628941 Msa0417440:CDS
ACTTACTGATTTCTTGAGGG+TGG 0.554227 3_2:+64627490 None:intergenic
TATTATATTAGGATCTTCGT+GGG 0.554686 3_2:-64626942 Msa0417440:three_prime_UTR
GGCTAGATGTTCAGATACAT+AGG 0.562753 3_2:+64628245 None:intergenic
GATACAAGCAGCAGAAACAT+TGG 0.565230 3_2:+64627526 None:intergenic
TATATCGAAACTAAGCTGGA+AGG 0.568673 3_2:-64627795 Msa0417440:CDS
GTAGCCTGTTGTTAGACCAA+TGG 0.580593 3_2:+64628224 None:intergenic
GGAAGGAAAAGTGAGAGTGG+TGG 0.583294 3_2:-64629699 Msa0417440:CDS
CTGCAAAATCCTTATTGGGT+GGG 0.587593 3_2:-64626738 Msa0417440:three_prime_UTR
TTGTTGAAACAAAGATGGGA+AGG 0.595444 3_2:+64629562 None:intergenic
ATATTTCGCATTTGACGCAG+TGG 0.596262 3_2:+64626766 None:intergenic
ATATTATATTAGGATCTTCG+TGG 0.596389 3_2:-64626943 Msa0417440:three_prime_UTR
ACTGCAAAATCCTTATTGGG+TGG 0.610141 3_2:-64626739 Msa0417440:three_prime_UTR
CTTAACGAGTGCGAAATTGG+AGG 0.610168 3_2:-64627587 Msa0417440:CDS
AAGATGGGAAGGTGTTCTTG+TGG 0.616586 3_2:+64629573 None:intergenic
ACGAGTGCGAAATTGGAGGA+GGG 0.618522 3_2:-64627583 Msa0417440:CDS
ATTTGTGTACAATCCTGCAA+TGG 0.626316 3_2:-64629637 Msa0417440:CDS
GGGAGCCTAACAACTTTCAG+CGG 0.627758 3_2:-64628198 Msa0417440:CDS
ATGGAGCGAACGAGCAGTTG+GGG 0.627866 3_2:-64629828 Msa0417440:CDS
CACCTAAGCCATACACCAAG+AGG 0.634181 3_2:+64627626 None:intergenic
GGTAGTGACCATAGTGCTGT+TGG 0.641933 3_2:-64627750 Msa0417440:CDS
GATCATCATTGGTTTCTGGA+CGG 0.641979 3_2:-64627876 Msa0417440:CDS
TCAATCACATTCTTGAAACA+GGG 0.655971 3_2:+64627090 None:intergenic
ATATAGTTATTATTAGATGG+CGG 0.673569 3_2:+64629603 None:intergenic
GTGTGTCAAAATGTAGATGT+TGG 0.674681 3_2:-64629783 Msa0417440:CDS
GAAGCTGAGAATCGTGAGTG+TGG 0.687844 3_2:-64629753 Msa0417440:CDS
TAAATATGCAGGTCTTAACG+AGG 0.700815 3_2:-64628973 Msa0417440:intron
TGGTCACTACCAATTGACGA+AGG 0.735981 3_2:+64627762 None:intergenic
GTGGTGGTGAAGCTCAGTTG+TGG 0.736786 3_2:-64627667 Msa0417440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTCCTAATATTAAAAA+TGG - chr3_2:64627942-64627961 Msa0417440:intron 10.0%
!!! TGTTATTTAATCTAATCTTT+AGG - chr3_2:64626511-64626530 Msa0417440:three_prime_UTR 15.0%
!! ACAATATAGTTATTATTAGA+TGG + chr3_2:64626892-64626911 None:intergenic 15.0%
!! TATATCATTAACAACAAAAA+AGG + chr3_2:64627006-64627025 None:intergenic 15.0%
!!! TTTATTTCTTTGAATTCTAA+AGG - chr3_2:64627661-64627680 Msa0417440:CDS 15.0%
!!! ATATTAGTAAATTTAGAGTA+TGG - chr3_2:64627765-64627784 Msa0417440:CDS 15.0%
!!! ATAGTTCCATTTTTAATATT+AGG + chr3_2:64627951-64627970 None:intergenic 15.0%
!!! AAAATTAAAAAGCATTTGAT+GGG + chr3_2:64628098-64628117 None:intergenic 15.0%
!!! AAAAATTAAAAAGCATTTGA+TGG + chr3_2:64628099-64628118 None:intergenic 15.0%
!!! TTGCTCTTTTTTATTTTAGA+AGG - chr3_2:64626605-64626624 Msa0417440:intron 20.0%
!!! AAGTTCTTATATATCTTTTG+AGG - chr3_2:64626632-64626651 Msa0417440:three_prime_UTR 20.0%
!! TTATATATCAAACCAGAAAA+TGG - chr3_2:64626826-64626845 Msa0417440:three_prime_UTR 20.0%
!! ATATAGTTATTATTAGATGG+CGG + chr3_2:64626889-64626908 None:intergenic 20.0%
!!! ATGCTTTTACTTTGTTTTTT+AGG - chr3_2:64627074-64627093 Msa0417440:three_prime_UTR 20.0%
!! AGAAATGTGTAAAAAATAGA+AGG + chr3_2:64627223-64627242 None:intergenic 20.0%
!! AATTTCAACAACAAATTAGA+CGG - chr3_2:64627320-64627339 Msa0417440:intron 20.0%
!! ATGTGTTAAATTATGTTTAG+TGG - chr3_2:64627357-64627376 Msa0417440:intron 20.0%
!! TGATTAATGAATATAACTAG+TGG - chr3_2:64627381-64627400 Msa0417440:intron 20.0%
!! TGCATATTTATTATGACAAT+TGG + chr3_2:64627506-64627525 None:intergenic 20.0%
!! AATTGTCATAATAAATATGC+AGG - chr3_2:64627505-64627524 Msa0417440:CDS 20.0%
!! GAAATAAATGAGTTCAAAAA+TGG - chr3_2:64628800-64628819 Msa0417440:intron 20.0%
!!! TGATGTGATATTTTTTCAAA+TGG + chr3_2:64629585-64629604 None:intergenic 20.0%
! ATCACTATGTTTCTTTCTAT+AGG + chr3_2:64626567-64626586 None:intergenic 25.0%
!!! TTACTTTGTTTTTTAGGTTC+TGG - chr3_2:64627080-64627099 Msa0417440:exon 25.0%
! TTTCTTGATGTTAAAGAGTA+TGG - chr3_2:64627238-64627257 Msa0417440:CDS 25.0%
! GGAAATATGTTAAATGAGTT+TGG - chr3_2:64627270-64627289 Msa0417440:CDS 25.0%
! TAATGAATATAACTAGTGGA+TGG - chr3_2:64627385-64627404 Msa0417440:intron 25.0%
! TACTTATTGCAAAAGTCATT+TGG - chr3_2:64627539-64627558 Msa0417440:CDS 25.0%
!! GAATTCTAAAGGAGAATAAA+AGG - chr3_2:64627672-64627691 Msa0417440:CDS 25.0%
! TAAAAGGAGAAAAATAACCA+TGG - chr3_2:64627688-64627707 Msa0417440:CDS 25.0%
! AAATAACCATGGTAGTTATA+AGG - chr3_2:64627699-64627718 Msa0417440:CDS 25.0%
!! TTATGATGGAAATGTCTATT+AGG - chr3_2:64627722-64627741 Msa0417440:CDS 25.0%
! TTATCATGATGACTAAATCT+TGG - chr3_2:64627788-64627807 Msa0417440:CDS 25.0%
! AAAGCAATTCAAGTCTTTAT+TGG - chr3_2:64627835-64627854 Msa0417440:CDS 25.0%
!!! ACTTCTATTTTTGCTTTCTT+AGG - chr3_2:64628049-64628068 Msa0417440:intron 25.0%
!! CTTTTATACATTTTCCAGAT+TGG - chr3_2:64628174-64628193 Msa0417440:CDS 25.0%
!!! TGGTTTGGTTTTGTTTTTAA+AGG - chr3_2:64628213-64628232 Msa0417440:CDS 25.0%
! CATATTAAGAAGAGTTTGTA+AGG - chr3_2:64628552-64628571 Msa0417440:intron 25.0%
! ATAAATGAGTTCAAAAATGG+TGG - chr3_2:64628803-64628822 Msa0417440:intron 25.0%
!!! AAAACTTACTGATTTCTTGA+GGG + chr3_2:64629005-64629024 None:intergenic 25.0%
!!! AAAAACTTACTGATTTCTTG+AGG + chr3_2:64629006-64629025 None:intergenic 25.0%
!! GAATTTTTGACACATGATTT+TGG - chr3_2:64629143-64629162 Msa0417440:intron 25.0%
!! ATTGCTATAAACCTGATTTT+TGG - chr3_2:64629180-64629199 Msa0417440:intron 25.0%
! AAAAGAAAATGAAACGCAAA+TGG + chr3_2:64629359-64629378 None:intergenic 25.0%
!! ATTTGCGTTTCATTTTCTTT+TGG - chr3_2:64629358-64629377 Msa0417440:CDS 25.0%
!! TTTGCGTTTCATTTTCTTTT+GGG - chr3_2:64629359-64629378 Msa0417440:CDS 25.0%
! GATTATGTACCTATTTGTTT+GGG - chr3_2:64629429-64629448 Msa0417440:intron 25.0%
! TTGTACAATAGTGAATTTGT+TGG - chr3_2:64629499-64629518 Msa0417440:intron 25.0%
!! TGTACAATAGTGAATTTGTT+GGG - chr3_2:64629500-64629519 Msa0417440:intron 25.0%
! GTTGGAAGAGATATTATATT+AGG - chr3_2:64629536-64629555 Msa0417440:CDS 25.0%
! ATATTATATTAGGATCTTCG+TGG - chr3_2:64629546-64629565 Msa0417440:CDS 25.0%
! TATTATATTAGGATCTTCGT+GGG - chr3_2:64629547-64629566 Msa0417440:CDS 25.0%
! AAAGAAACATTGTTCTTGTT+TGG - chr3_2:64629882-64629901 Msa0417440:five_prime_UTR 25.0%
!!! TTTTCGAGTTATTTTTCGGA+AGG - chr3_2:64626477-64626496 Msa0417440:three_prime_UTR 30.0%
! CTGGTTTGATATATAAAGAC+AGG + chr3_2:64626822-64626841 None:intergenic 30.0%
! TACACAAATGTTCCATTTTC+TGG + chr3_2:64626841-64626860 None:intergenic 30.0%
! CCATTTGTTGAAACAAAGAT+GGG + chr3_2:64626934-64626953 None:intergenic 30.0%
! TCCATTTGTTGAAACAAAGA+TGG + chr3_2:64626935-64626954 None:intergenic 30.0%
! CTTTGTTTCAACAAATGGAA+AGG - chr3_2:64626936-64626955 Msa0417440:three_prime_UTR 30.0%
TGTGAAGACATAAAACAAGT+AGG - chr3_2:64626964-64626983 Msa0417440:three_prime_UTR 30.0%
! AAATTGTACTAGTTTCCTCA+CGG - chr3_2:64627044-64627063 Msa0417440:three_prime_UTR 30.0%
TCATAACCTTATAACTACCA+TGG + chr3_2:64627708-64627727 None:intergenic 30.0%
TGGTAGTTATAAGGTTATGA+TGG - chr3_2:64627708-64627727 Msa0417440:CDS 30.0%
!! ACTTGAATTGCTTTTCAATC+AGG + chr3_2:64627829-64627848 None:intergenic 30.0%
TTGCTTTCTTAGGTTCAATA+TGG - chr3_2:64628059-64628078 Msa0417440:intron 30.0%
ATAAGTGCGATTTAAAGACT+AGG - chr3_2:64628388-64628407 Msa0417440:intron 30.0%
! TCTAATCTCATTTTACAGCA+TGG + chr3_2:64628499-64628518 None:intergenic 30.0%
AAGAAGAGTTTGTAAGGAAA+TGG - chr3_2:64628558-64628577 Msa0417440:intron 30.0%
! GTAAGGAAATGGAATTTTGT+AGG - chr3_2:64628569-64628588 Msa0417440:intron 30.0%
! CTTTTATATCGAAACTAAGC+TGG - chr3_2:64628690-64628709 Msa0417440:intron 30.0%
!! TGGTGATATTAGCTTTGTTA+TGG - chr3_2:64628759-64628778 Msa0417440:intron 30.0%
!! GGTGATATTAGCTTTGTTAT+GGG - chr3_2:64628760-64628779 Msa0417440:intron 30.0%
!! GTGATATTAGCTTTGTTATG+GGG - chr3_2:64628761-64628780 Msa0417440:intron 30.0%
!!! TTTTTGAAATGGCTTCGATT+TGG - chr3_2:64628929-64628948 Msa0417440:CDS 30.0%
!!! TTTTGAAATGGCTTCGATTT+GGG - chr3_2:64628930-64628949 Msa0417440:CDS 30.0%
! TTTTTGACACATGATTTTGG+AGG - chr3_2:64629146-64629165 Msa0417440:intron 30.0%
AATTGTGATACTGTGATAAC+AGG - chr3_2:64629220-64629239 Msa0417440:intron 30.0%
TGATAGAAAACAAGCATCTT+TGG - chr3_2:64629308-64629327 Msa0417440:intron 30.0%
TCAATCACATTCTTGAAACA+GGG + chr3_2:64629402-64629421 None:intergenic 30.0%
GGATTATGTACCTATTTGTT+TGG - chr3_2:64629428-64629447 Msa0417440:intron 30.0%
TTGAACTGCAAAATCCTTAT+TGG - chr3_2:64629746-64629765 Msa0417440:CDS 30.0%
TGAACTGCAAAATCCTTATT+GGG - chr3_2:64629747-64629766 Msa0417440:CDS 30.0%
!!! TTTCTCTAGCTTTTTGATGA+GGG - chr3_2:64629917-64629936 Msa0417440:five_prime_UTR 30.0%
! TTGACATTTAAATTTGCTCC+CGG - chr3_2:64629976-64629995 Msa0417440:five_prime_UTR 30.0%
GTGTGTCAAAATGTAGATGT+TGG - chr3_2:64626706-64626725 Msa0417440:three_prime_UTR 35.0%
ATTTGTGTACAATCCTGCAA+TGG - chr3_2:64626852-64626871 Msa0417440:three_prime_UTR 35.0%
TTGTTGAAACAAAGATGGGA+AGG + chr3_2:64626930-64626949 None:intergenic 35.0%
! CCCATCTTTGTTTCAACAAA+TGG - chr3_2:64626931-64626950 Msa0417440:three_prime_UTR 35.0%
GAAACAAATTCCAGTAGCTT+GGG + chr3_2:64627123-64627142 None:intergenic 35.0%
TGTTTCGTGTCTAACTCATT+TGG - chr3_2:64627137-64627156 Msa0417440:CDS 35.0%
!! CTAACTCATTTGGCTGTTTT+TGG - chr3_2:64627147-64627166 Msa0417440:CDS 35.0%
! TAAAGAGTATGGTAGATGCT+AGG - chr3_2:64627249-64627268 Msa0417440:CDS 35.0%
CTACTCGACGCAATTAAATT+AGG - chr3_2:64627444-64627463 Msa0417440:intron 35.0%
TAAATATGCAGGTCTTAACG+AGG - chr3_2:64627516-64627535 Msa0417440:CDS 35.0%
GTAAAAAACGGTATGATCAC+TGG + chr3_2:64627585-64627604 None:intergenic 35.0%
TGGCAGATCAAGGTAAAAAA+CGG + chr3_2:64627597-64627616 None:intergenic 35.0%
TAAGAATCAGTGAATGCAGA+AGG + chr3_2:64627639-64627658 None:intergenic 35.0%
TGATGGAAATGTCTATTAGG+TGG - chr3_2:64627725-64627744 Msa0417440:CDS 35.0%
AAAATGGAACTATCTGTTCC+AGG - chr3_2:64627958-64627977 Msa0417440:intron 35.0%
!! CAGATTGGTTCCTTTTTGAT+GGG - chr3_2:64628189-64628208 Msa0417440:CDS 35.0%
TATATCGAAACTAAGCTGGA+AGG - chr3_2:64628694-64628713 Msa0417440:intron 35.0%
TAATATCACCAACAGCACTA+TGG + chr3_2:64628750-64628769 None:intergenic 35.0%
AATGAGTTCAAAAATGGTGG+TGG - chr3_2:64628806-64628825 Msa0417440:intron 35.0%
!!! ATAAACCTGATTTTTGGTGC+AGG - chr3_2:64629186-64629205 Msa0417440:intron 35.0%
GTGATAACAGGACTTCAATT+TGG - chr3_2:64629232-64629251 Msa0417440:intron 35.0%
AGGACTTCAATTTGGTCTAT+TGG - chr3_2:64629240-64629259 Msa0417440:intron 35.0%
GGACTTCAATTTGGTCTATT+GGG - chr3_2:64629241-64629260 Msa0417440:intron 35.0%
CTCAATCACATTCTTGAAAC+AGG + chr3_2:64629403-64629422 None:intergenic 35.0%
TCAAGAATGTGATTGAGCAT+AGG - chr3_2:64629406-64629425 Msa0417440:intron 35.0%
CAAGAATGTGATTGAGCATA+GGG - chr3_2:64629407-64629426 Msa0417440:intron 35.0%
TACCTATTTGTTTGGGTGTT+TGG - chr3_2:64629436-64629455 Msa0417440:intron 35.0%
TACATTCTCGCTTCGAAAAA+AGG - chr3_2:64629803-64629822 Msa0417440:CDS 35.0%
!!! CTTTCTCTAGCTTTTTGATG+AGG - chr3_2:64629916-64629935 Msa0417440:five_prime_UTR 35.0%
!! ATATCTTTTGAGGATCGCCA+TGG - chr3_2:64626642-64626661 Msa0417440:three_prime_UTR 40.0%
!! AAATGGCTTTTCAAGGAGGA+AGG - chr3_2:64626773-64626792 Msa0417440:three_prime_UTR 40.0%
CGAAACAAATTCCAGTAGCT+TGG + chr3_2:64627124-64627143 None:intergenic 40.0%
!!! TTGGCTGTTTTTGGCATTCT+TGG - chr3_2:64627156-64627175 Msa0417440:CDS 40.0%
!!! TGGCTGTTTTTGGCATTCTT+GGG - chr3_2:64627157-64627176 Msa0417440:CDS 40.0%
TGCGTCGAGTAGATGATTAA+TGG + chr3_2:64627436-64627455 None:intergenic 40.0%
CGACGCAATTAAATTAGGAG+AGG - chr3_2:64627449-64627468 Msa0417440:intron 40.0%
CAAAAGTCATTTGGACCAAG+TGG - chr3_2:64627548-64627567 Msa0417440:CDS 40.0%
! TCATTTGGACCAAGTGGTTT+TGG - chr3_2:64627554-64627573 Msa0417440:CDS 40.0%
! CCATATTTGATGGCAGATCA+AGG + chr3_2:64627607-64627626 None:intergenic 40.0%
CCTTGATCTGCCATCAAATA+TGG - chr3_2:64627604-64627623 Msa0417440:CDS 40.0%
GAGAGGCAAACCATATTTGA+TGG + chr3_2:64627617-64627636 None:intergenic 40.0%
GCTGCATTAGCTAACATGTT+TGG - chr3_2:64627862-64627881 Msa0417440:CDS 40.0%
AATGCTTCAAGCTCAAAACC+TGG + chr3_2:64627979-64627998 None:intergenic 40.0%
CCATCAAAAAGGAACCAATC+TGG + chr3_2:64628191-64628210 None:intergenic 40.0%
!! CCAGATTGGTTCCTTTTTGA+TGG - chr3_2:64628188-64628207 Msa0417440:CDS 40.0%
! TTGGTTCCTTTTTGATGGGA+TGG - chr3_2:64628193-64628212 Msa0417440:CDS 40.0%
! TCCTTTTTGATGGGATGGTT+TGG - chr3_2:64628198-64628217 Msa0417440:CDS 40.0%
ACCAAACCATCCCATCAAAA+AGG + chr3_2:64628202-64628221 None:intergenic 40.0%
GGCTAGATGTTCAGATACAT+AGG + chr3_2:64628247-64628266 None:intergenic 40.0%
GTATCTGAACATCTAGCCAT+TGG - chr3_2:64628249-64628268 Msa0417440:CDS 40.0%
GTCTAACAACAGGCTACTTA+GGG - chr3_2:64628271-64628290 Msa0417440:CDS 40.0%
GTAGCATTGTCGATCATCAT+TGG - chr3_2:64628602-64628621 Msa0417440:intron 40.0%
!! TGTCGATCATCATTGGTTTC+TGG - chr3_2:64628609-64628628 Msa0417440:intron 40.0%
!! GATCATCATTGGTTTCTGGA+CGG - chr3_2:64628613-64628632 Msa0417440:intron 40.0%
AGACTTAACGAGTGCGAAAT+TGG - chr3_2:64628899-64628918 Msa0417440:CDS 40.0%
!! TTGGAGGAGGGTTTTTGAAA+TGG - chr3_2:64628918-64628937 Msa0417440:CDS 40.0%
GATACAAGCAGCAGAAACAT+TGG + chr3_2:64628966-64628985 None:intergenic 40.0%
! TGTTTCTGCTGCTTGTATCA+TGG - chr3_2:64628967-64628986 Msa0417440:intron 40.0%
!! ACTTACTGATTTCTTGAGGG+TGG + chr3_2:64629002-64629021 None:intergenic 40.0%
ATTCACCTGCACCAAAAATC+AGG + chr3_2:64629194-64629213 None:intergenic 40.0%
!! GAGAGCATTTGTCTATGCTA+TGG - chr3_2:64629330-64629349 Msa0417440:intron 40.0%
AGCCAAACACCCAAACAAAT+AGG + chr3_2:64629441-64629460 None:intergenic 40.0%
!! GAATTTGTTGGGTCTGAGTT+AGG - chr3_2:64629511-64629530 Msa0417440:intron 40.0%
ATTAGGATCTTCGTGGGATT+TGG - chr3_2:64629553-64629572 Msa0417440:CDS 40.0%
ATATTTCGCATTTGACGCAG+TGG + chr3_2:64629726-64629745 None:intergenic 40.0%
ACTGCAAAATCCTTATTGGG+TGG - chr3_2:64629750-64629769 Msa0417440:CDS 40.0%
CTGCAAAATCCTTATTGGGT+GGG - chr3_2:64629751-64629770 Msa0417440:CDS 40.0%
AACAATAGTCCCACCCAATA+AGG + chr3_2:64629763-64629782 None:intergenic 40.0%
!!! TCTAGCTTTTTGATGAGGGT+GGG - chr3_2:64629921-64629940 Msa0417440:five_prime_UTR 40.0%
! GCAGGAGAAATGGCTTTTCA+AGG - chr3_2:64626766-64626785 Msa0417440:three_prime_UTR 45.0%
!! GGAGAAATGGCTTTTCAAGG+AGG - chr3_2:64626769-64626788 Msa0417440:three_prime_UTR 45.0%
GGCAATTCATGTTCCATTGC+AGG + chr3_2:64626868-64626887 None:intergenic 45.0%
!! AAGATGGGAAGGTGTTCTTG+TGG + chr3_2:64626919-64626938 None:intergenic 45.0%
! TACTAGTTTCCTCACGGTTG+AGG - chr3_2:64627050-64627069 Msa0417440:three_prime_UTR 45.0%
CATGAAGTTACCCAAGCTAC+TGG - chr3_2:64627110-64627129 Msa0417440:CDS 45.0%
!!! GGCTGTTTTTGGCATTCTTG+GGG - chr3_2:64627158-64627177 Msa0417440:CDS 45.0%
ATCAGTGAATGCAGAAGGAG+AGG + chr3_2:64627634-64627653 None:intergenic 45.0%
! CTAGCCATTGGTCTAACAAC+AGG - chr3_2:64628261-64628280 Msa0417440:CDS 45.0%
GTAGCCTGTTGTTAGACCAA+TGG + chr3_2:64628268-64628287 None:intergenic 45.0%
GGTCTAACAACAGGCTACTT+AGG - chr3_2:64628270-64628289 Msa0417440:CDS 45.0%
CACTGGATCTTGTCTTTGCT+CGG - chr3_2:64628351-64628370 Msa0417440:intron 45.0%
! TCTTTGCTCGGCTTTGTACT+AGG - chr3_2:64628363-64628382 Msa0417440:intron 45.0%
TGACAGCTTCCTTCGTCAAT+TGG - chr3_2:64628718-64628737 Msa0417440:intron 45.0%
TGGTCACTACCAATTGACGA+AGG + chr3_2:64628730-64628749 None:intergenic 45.0%
!! TTGTGGTTAGCCTGCATTGT+TGG - chr3_2:64628839-64628858 Msa0417440:intron 45.0%
! TTGTTGGACCTCTTGGTGTA+TGG - chr3_2:64628855-64628874 Msa0417440:intron 45.0%
CTTAACGAGTGCGAAATTGG+AGG - chr3_2:64628902-64628921 Msa0417440:CDS 45.0%
TTGGGTCTGAGTTAGGAAGT+TGG - chr3_2:64629518-64629537 Msa0417440:intron 45.0%
!!! CTCTAGCTTTTTGATGAGGG+TGG - chr3_2:64629920-64629939 Msa0417440:five_prime_UTR 45.0%
GAAGCTGAGAATCGTGAGTG+TGG - chr3_2:64626736-64626755 Msa0417440:three_prime_UTR 50.0%
TGGTTCAGCTGCAGGAGAAA+TGG - chr3_2:64626756-64626775 Msa0417440:three_prime_UTR 50.0%
GGAGGAAGGAAAAGTGAGAG+TGG - chr3_2:64626787-64626806 Msa0417440:three_prime_UTR 50.0%
GGAAGGAAAAGTGAGAGTGG+TGG - chr3_2:64626790-64626809 Msa0417440:three_prime_UTR 50.0%
! AAAAGCATGCCTCAACCGTG+AGG + chr3_2:64627062-64627081 None:intergenic 50.0%
GGGAGCCTAACAACTTTCAG+CGG - chr3_2:64628291-64628310 Msa0417440:CDS 50.0%
TCCAGCCGCTGAAAGTTGTT+AGG + chr3_2:64628299-64628318 None:intergenic 50.0%
! ATTGGTTTCTGGACGGCGAA+GGG - chr3_2:64628620-64628639 Msa0417440:intron 50.0%
GGTAGTGACCATAGTGCTGT+TGG - chr3_2:64628739-64628758 Msa0417440:intron 50.0%
ATGGGGTGTGAGTAGTGCTT+TGG - chr3_2:64628778-64628797 Msa0417440:intron 50.0%
ACCAAGAGGTCCAACAATGC+AGG + chr3_2:64628852-64628871 None:intergenic 50.0%
! GACCTCTTGGTGTATGGCTT+AGG - chr3_2:64628861-64628880 Msa0417440:intron 50.0%
CACCTAAGCCATACACCAAG+AGG + chr3_2:64628866-64628885 None:intergenic 50.0%
! CTCTTGGTGTATGGCTTAGG+TGG - chr3_2:64628864-64628883 Msa0417440:intron 50.0%
AACGAGTGCGAAATTGGAGG+AGG - chr3_2:64628905-64628924 Msa0417440:CDS 50.0%
ACGAGTGCGAAATTGGAGGA+GGG - chr3_2:64628906-64628925 Msa0417440:CDS 50.0%
!! AAGTTGACTCGACTCAGTCC+GGG + chr3_2:64629997-64630016 None:intergenic 50.0%
!! GAAGTTGACTCGACTCAGTC+CGG + chr3_2:64629998-64630017 None:intergenic 50.0%
CATGGAGCGAACGAGCAGTT+GGG - chr3_2:64626660-64626679 Msa0417440:three_prime_UTR 55.0%
ATGGAGCGAACGAGCAGTTG+GGG - chr3_2:64626661-64626680 Msa0417440:three_prime_UTR 55.0%
GTTGGGGTTCTGCAGTTAGC+AGG - chr3_2:64626677-64626696 Msa0417440:three_prime_UTR 55.0%
CTGGTCACGCCAAAACCACT+TGG + chr3_2:64627566-64627585 None:intergenic 55.0%
GCCTAACAACTTTCAGCGGC+TGG - chr3_2:64628295-64628314 Msa0417440:CDS 55.0%
!! CATTGGTTTCTGGACGGCGA+AGG - chr3_2:64628619-64628638 Msa0417440:intron 55.0%
GGACGGCGAAGGGATTCAAA+TGG - chr3_2:64628630-64628649 Msa0417440:intron 55.0%
! GTGGTGGTGAAGCTCAGTTG+TGG - chr3_2:64628822-64628841 Msa0417440:intron 55.0%
GCCTGCATTGTTGGACCTCT+TGG - chr3_2:64628848-64628867 Msa0417440:intron 55.0%
CCAACTGCTCGTTCGCTCCA+TGG + chr3_2:64626662-64626681 None:intergenic 60.0%
CCATGGAGCGAACGAGCAGT+TGG - chr3_2:64626659-64626678 Msa0417440:three_prime_UTR 60.0%
CGTGAGTGTGGTTCAGCTGC+AGG - chr3_2:64626748-64626767 Msa0417440:three_prime_UTR 60.0%
ATGCTCGAGCTCAGTGTCGC+TGG - chr3_2:64628327-64628346 Msa0417440:intron 60.0%
! AGCTCAGTGTCGCTGGACAC+TGG - chr3_2:64628334-64628353 Msa0417440:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 64626474 64630037 64626474 ID=Msa0417440;Name=Msa0417440
chr3_2 mRNA 64626474 64630037 64626474 ID=Msa0417440-mRNA-1;Parent=Msa0417440;Name=Msa0417440-mRNA-1;_AED=0.31;_eAED=0.31;_QI=187|1|1|1|0.83|0.71|7|593|443
chr3_2 exon 64626474 64626585 64626474 ID=Msa0417440-mRNA-1:exon:14380;Parent=Msa0417440-mRNA-1
chr3_2 exon 64626613 64627303 64626613 ID=Msa0417440-mRNA-1:exon:14379;Parent=Msa0417440-mRNA-1
chr3_2 exon 64627496 64627920 64627496 ID=Msa0417440-mRNA-1:exon:14378;Parent=Msa0417440-mRNA-1
chr3_2 exon 64628127 64628319 64628127 ID=Msa0417440-mRNA-1:exon:14377;Parent=Msa0417440-mRNA-1
chr3_2 exon 64628886 64628984 64628886 ID=Msa0417440-mRNA-1:exon:14376;Parent=Msa0417440-mRNA-1
chr3_2 exon 64629332 64629415 64629332 ID=Msa0417440-mRNA-1:exon:14375;Parent=Msa0417440-mRNA-1
chr3_2 exon 64629530 64630037 64629530 ID=Msa0417440-mRNA-1:exon:14374;Parent=Msa0417440-mRNA-1
chr3_2 five_prime_UTR 64629851 64630037 64629851 ID=Msa0417440-mRNA-1:five_prime_utr;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64629530 64629850 64629530 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64629332 64629415 64629332 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64628886 64628984 64628886 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64628127 64628319 64628127 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64627496 64627920 64627496 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 CDS 64627094 64627303 64627094 ID=Msa0417440-mRNA-1:cds;Parent=Msa0417440-mRNA-1
chr3_2 three_prime_UTR 64626613 64627093 64626613 ID=Msa0417440-mRNA-1:three_prime_utr;Parent=Msa0417440-mRNA-1
chr3_2 three_prime_UTR 64626474 64626585 64626474 ID=Msa0417440-mRNA-1:three_prime_utr;Parent=Msa0417440-mRNA-1
Gene Sequence

>Msa0417440

ATGGAGCGAACGAGCAGTTGGGGTTCTGCAGTTAGCAGGCTTTCAGTGTGTCAAAATGTAGATGTTGGTGATGATGAAGCTGAGAATCGTGAGTGTGGTTCAGCTGCAGGAGAAATGGCTTTTCAAGGAGGAAGGAAAAGTGAGAGTGGTGGCTTTGACCTGTCTTTATATATCAAACCAGAAAATGGAACATTTGTGTACAATCCTGCAATGGAACATGAATTGCCGCCATCTAATAATAACTATATTGTAAATCCACAAGAACACCTTCCCATCTTTGTTTCAACAAATGGAAAGGTTAATTGTGAAGACATAAAACAAGTTCTGGAACTTGACATGAAGTTACCCAAGCTACTGGAATTTGTTTCGTGTCTAACTCATTTGGCTGTTTTTGGCATTCTTGGGGTCTTAACGAGGTACTTATTGCAAAAGTCATTTGGACCAAGTGGTTTTGGCGTGACCAGTGATCATACCGTTTTTTACCTTGATCTGCCATCAAATATGATTGGTTCCTTTTTGATGGGATGGTTTGGTTTTGTTTTTAAAGGAGATATATCCTATGTATCTGAACATCTAGCCATTGGTCTAACAACAGGCTACTTAGGGAGCCTAACAACTTTCAGCGGCTGGAATCAGAAAATGCTCGAGCTCAGTGTCGCTGGACACTGGATCTTGTCTTTGCTCGGCTTTGTACTAGGCATGTTGCTTGTAGCATTGTCGATCATCATTGGTTTCTGGACGGCGAAGGGATTCAAATGGTTACTCAAAAGACTAAACATGAGACATGAAAGTGACTCTTTTATATCGAAACTAAGCTGGAAGGTTGACAGCTTCCTTCGTCAATTGGTAGTGACCATAGTGCTGTTGGTGATATTAGCTTTGTTATGGGGTGTGAGTAGTGCTTTGGAAATAAATGAGTTCAAAAATGGTGGTGGTGAAGCTCAGTTGTGGTTAGCCTGCATTGTTGGACCTCTTGGTGTATGGCTTAGGTGGTTCTTAGCTACTAGACTTAACGAGTGCGAAATTGGAGGAGGGTTTTTGAAATGGCTTCGATTTGGGACTCTTATTGCCAATGTTTCTGCTGCTTGTATCATGGCTGCACTTGCCACCCTCAAGAAATCAGTGAATACTACAAATTGTGATACTGTGATAACAGGACTTCAATTTGGTCTATTGGGATGTCTGAGTACTGTTTCAACATTTGTTGCTGAATTGTATGAAATGATAGAAAACAAGCATCTTTGGAGAGCATTTGTCTATGCTATGGTTACCATTTGCGTTTCATTTTCTTTTGGGATCTTATTATACTATATCCCTGTTTCAAGAATGTGA
Protein sequence

>Msa0417440

MERTSSWGSAVSRLSVCQNVDVGDDEAENRECGSAAGEMAFQGGRKSESGGFDLSLYIKPENGTFVYNPAMEHELPPSNNNYIVNPQEHLPIFVSTNGKVNCEDIKQVLELDMKLPKLLEFVSCLTHLAVFGILGVLTRYLLQKSFGPSGFGVTSDHTVFYLDLPSNMIGSFLMGWFGFVFKGDISYVSEHLAIGLTTGYLGSLTTFSGWNQKMLELSVAGHWILSLLGFVLGMLLVALSIIIGFWTAKGFKWLLKRLNMRHESDSFISKLSWKVDSFLRQLVVTIVLLVILALLWGVSSALEINEFKNGGGEAQLWLACIVGPLGVWLRWFLATRLNECEIGGGFLKWLRFGTLIANVSAACIMAALATLKKSVNTTNCDTVITGLQFGLLGCLSTVSTFVAELYEMIENKHLWRAFVYAMVTICVSFSFGILLYYIPVSRM*