AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0509790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509790 MtrunA17_Chr3g0114931 93.034 445 23 3 1 443 1 439 0.0 799
Msa0509790 MtrunA17_Chr3g0114921 54.788 449 185 9 1 439 1 441 3.32e-152 439
Msa0509790 MtrunA17_Chr3g0078481 64.183 349 120 4 96 443 36 380 3.00e-143 414
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0509790 AT2G41705.3 51.529 425 191 7 25 440 37 455 3.89e-120 358
Msa0509790 AT2G41705.2 51.529 425 191 7 25 440 37 455 3.89e-120 358
Msa0509790 AT2G41705.4 51.529 425 191 7 25 440 37 455 3.89e-120 358
Msa0509790 AT2G41705.1 51.529 425 191 7 25 440 37 455 3.89e-120 358

Find 112 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGACTTCAATTTGGTCTAT+TGG 0.243981 3_4:-58378640 Msa0509790:CDS
TGTCGATCATCATTGGTTTC+TGG 0.244597 3_4:-58379271 Msa0509790:CDS
ATTAGGATCTTCGTGGGATT+TGG 0.260829 3_4:-58378294 Msa0509790:three_prime_UTR
TACCTATTTGTTTGGGTGTT+TGG 0.268786 3_4:-58378444 Msa0509790:three_prime_UTR
TGGTGATATTAGCTTTGTTA+TGG 0.283155 3_4:-58379121 Msa0509790:CDS
CCCATCTTTGTTTCAACAAA+TGG 0.283826 3_4:-58380938 Msa0509790:CDS
GCCTGCATTGTTGGACCTCT+TGG 0.293298 3_4:-58379032 Msa0509790:CDS
GTTGGAAGAGATATTATATT+AGG 0.293744 3_4:-58378344 Msa0509790:three_prime_UTR
CCTTGATCTGCCATCAAATA+TGG 0.293851 3_4:-58380266 Msa0509790:intron
AGACTTAACGAGTGCGAAAT+TGG 0.297010 3_4:-58378981 Msa0509790:CDS
TGTTATTTAATCTAATCTTT+AGG 0.299381 3_4:-58381358 Msa0509790:five_prime_UTR
TCATTTGGACCAAGTGGTTT+TGG 0.299453 3_4:-58380316 Msa0509790:CDS
GGATTATGTACCTATTTGTT+TGG 0.307962 3_4:-58378452 Msa0509790:three_prime_UTR
TGATAGAAAACAAGCATCTT+TGG 0.308824 3_4:-58378572 Msa0509790:CDS
AAAGAAACATTGTTCTTGTT+TGG 0.311813 3_4:-58377908 Msa0509790:three_prime_UTR
GGACTTCAATTTGGTCTATT+GGG 0.318154 3_4:-58378639 Msa0509790:CDS
CTCACTCACATTCTTGAAAC+AGG 0.318173 3_4:+58378480 None:intergenic
ACAATATAGTTATTATTAGA+TGG 0.318561 3_4:+58380980 None:intergenic
TTATATATCAAACCAGAAAA+TGG 0.319807 3_4:-58381043 Msa0509790:CDS
GTGATAACAGGACTTCAATT+TGG 0.320323 3_4:-58378648 Msa0509790:CDS
CAGCTTAAGTTAGGTATAAC+AGG 0.325015 3_4:-58381424 Msa0509790:five_prime_UTR
ACCAAACCATCCCATCAAAA+AGG 0.332116 3_4:+58379719 None:intergenic
TACTTATTGCAAAAGTCATT+TGG 0.339763 3_4:-58380331 Msa0509790:CDS
GGTGATATTAGCTTTGTTAT+GGG 0.342133 3_4:-58379120 Msa0509790:CDS
GATTATGTACCTATTTGTTT+GGG 0.342788 3_4:-58378451 Msa0509790:three_prime_UTR
GGCAATTCATGTTCCATTGC+AGG 0.342935 3_4:+58381004 None:intergenic
CACTGGATCTTGTCTTTGCT+CGG 0.345396 3_4:-58379567 Msa0509790:CDS
ATTAACTTGCAGCTTAAGTT+AGG 0.354555 3_4:-58381433 Msa0509790:five_prime_UTR
ATCACTATGTTTCTTTCTAT+AGG 0.370649 3_4:+58381305 None:intergenic
TGAACTACAAGATTCTTATT+CGG 0.373116 3_4:-58378079 Msa0509790:three_prime_UTR
TGTTTCGTGTCTAACTCATT+TGG 0.377439 3_4:-58380732 Msa0509790:CDS
TTGTTGGACCTCTTGGTGTA+TGG 0.380313 3_4:-58379025 Msa0509790:CDS
GAATTTGTTGGGTCTGAGTT+AGG 0.382110 3_4:-58378369 Msa0509790:three_prime_UTR
GACCTCTTGGTGTATGGCTT+AGG 0.383143 3_4:-58379019 Msa0509790:CDS
TCCATTTGTTGAAACAAAGA+TGG 0.386494 3_4:+58380937 None:intergenic
GGTCTAACAACCGGCTACTT+AGG 0.389772 3_4:-58379648 Msa0509790:CDS
TCTTTGCTCGGCTTTGTACT+AGG 0.391256 3_4:-58379555 Msa0509790:intron
TTTCAATACATTCTTCCCTT+AGG 0.398094 3_4:-58378029 Msa0509790:three_prime_UTR
AATTGTGATACTGTGATAAC+AGG 0.402053 3_4:-58378660 Msa0509790:CDS
AGCCAAACACCCAAACAAAT+AGG 0.425540 3_4:+58378442 None:intergenic
CATGGAGCGAACGAGCAGTT+GGG 0.430438 3_4:-58381209 Msa0509790:CDS
TGACAGCTTCCTTCGTCAAT+TGG 0.430603 3_4:-58379162 Msa0509790:CDS
AAAACTTACTGATTTCTTGA+GGG 0.431062 3_4:+58378878 None:intergenic
GTTGGGGTTCTGCAGTTAGC+AGG 0.434224 3_4:-58381192 Msa0509790:CDS
CGTGAGTGTGGTTCAGCTGC+AGG 0.439210 3_4:-58381121 Msa0509790:CDS
GCCTAACAACTTTCAGCGGC+TGG 0.451556 3_4:-58379623 Msa0509790:CDS
TCCAGCCGCTGAAAGTTGTT+AGG 0.453775 3_4:+58379622 None:intergenic
CTGGTTTGATATATAAAGAC+AGG 0.458399 3_4:+58381050 None:intergenic
GTAGCATTGTCGATCATCAT+TGG 0.460693 3_4:-58379278 Msa0509790:CDS
TTGTGGTTAGCCTGCATTGT+TGG 0.460887 3_4:-58379041 Msa0509790:CDS
ATGCTCGAGCTCAGTGTCGC+TGG 0.460917 3_4:-58379591 Msa0509790:CDS
CGAAACAAATTCCAGTAGCT+TGG 0.472897 3_4:+58380748 None:intergenic
GTCTAACAACCGGCTACTTA+GGG 0.474314 3_4:-58379647 Msa0509790:CDS
CTAGCCATTGGTCTAACAAC+CGG 0.476478 3_4:-58379657 Msa0509790:CDS
AGCTCAGTGTCGCTGGACAC+TGG 0.485522 3_4:-58379584 Msa0509790:CDS
ATGGGGTGTGAGTAGTGCTT+TGG 0.485675 3_4:-58379102 Msa0509790:CDS
CATGAAGTTACCCAAGCTAC+TGG 0.489022 3_4:-58380759 Msa0509790:CDS
TAATATCACCAACAACACTA+TGG 0.492265 3_4:+58379133 None:intergenic
TGTACAATAGTGAATTTGTT+GGG 0.492567 3_4:-58378380 Msa0509790:three_prime_UTR
TTGGGTCTGAGTTAGGAAGT+TGG 0.493066 3_4:-58378362 Msa0509790:three_prime_UTR
AAAATGATGTATCATACAAA+AGG 0.493771 3_4:+58381491 None:intergenic
GAGAGCATTTGTCTATGCTA+TGG 0.494452 3_4:-58378550 Msa0509790:CDS
CAAGAATGTGAGTGAGCATA+GGG 0.494592 3_4:-58378473 Msa0509790:exon
CCAACTGCTCGTTCGCTCCA+TGG 0.497205 3_4:+58381210 None:intergenic
CCATGGAGCGAACGAGCAGT+TGG 0.498043 3_4:-58381210 Msa0509790:CDS
AAAAGAAAATGAAACGCAAA+TGG 0.498184 3_4:+58378524 None:intergenic
GAGAGGCGGACCATATTTGA+TGG 0.502691 3_4:+58380256 None:intergenic
GAAACAAATTCCAGTAGCTT+GGG 0.506298 3_4:+58380749 None:intergenic
CTTTGTTTCAACAAATGGAA+AGG 0.511711 3_4:-58380933 Msa0509790:CDS
CATTGGTTTCTGGACGGCGA+AGG 0.512919 3_4:-58379261 Msa0509790:CDS
GGTAGTGACCATAGTGTTGT+TGG 0.519775 3_4:-58379141 Msa0509790:CDS
GTGATATTAGCTTTGTTATG+GGG 0.520721 3_4:-58379119 Msa0509790:CDS
AACGAGTGCGAAATTGGAGG+AGG 0.526946 3_4:-58378975 Msa0509790:CDS
GGAGGAAGGAAAAGTGAGAG+TGG 0.527956 3_4:-58381082 Msa0509790:CDS
CCATATTTGATGGCAGATCA+AGG 0.529014 3_4:+58380266 None:intergenic
CCATTTGTTGAAACAAAGAT+GGG 0.530182 3_4:+58380938 None:intergenic
TTGTACAATAGTGAATTTGT+TGG 0.531782 3_4:-58378381 Msa0509790:three_prime_UTR
GACACATATTAGGATCTTCG+TGG 0.534243 3_4:-58378301 Msa0509790:three_prime_UTR
ACTACAAGATTCTTATTCGG+TGG 0.536500 3_4:-58378076 Msa0509790:three_prime_UTR
ATTGGTTTCTGGACGGCGAA+GGG 0.538719 3_4:-58379260 Msa0509790:CDS
TGTTTCTGCTGCTTGTATCA+CGG 0.540208 3_4:-58378913 Msa0509790:CDS
GGACGGCGAAGGGATTCAAA+TGG 0.542568 3_4:-58379250 Msa0509790:CDS
TCAAGAATGTGAGTGAGCAT+AGG 0.546189 3_4:-58378474 Msa0509790:exon
ACCAAGAGGTCCAACAATGC+AGG 0.546644 3_4:+58379031 None:intergenic
CTGGTCACGCCAAAACCACT+TGG 0.550500 3_4:+58380307 None:intergenic
CAAAAGTCATTTGGACCAAG+TGG 0.552223 3_4:-58380322 Msa0509790:CDS
ACTTACTGATTTCTTGAGGG+TGG 0.554227 3_4:+58378881 None:intergenic
TGTTAGGCTCCCTAAGTAGC+CGG 0.558390 3_4:+58379638 None:intergenic
GTATCTGAACATCTAGCCAT+TGG 0.561646 3_4:-58379669 Msa0509790:CDS
CTACAAGATTCTTATTCGGT+GGG 0.561724 3_4:-58378075 Msa0509790:three_prime_UTR
TATATCGAAACTAAGCTGGA+AGG 0.568673 3_4:-58379186 Msa0509790:CDS
ACACATATTAGGATCTTCGT+GGG 0.576402 3_4:-58378300 Msa0509790:three_prime_UTR
GGAAGGAAAAGTGAGAGTGG+TGG 0.583294 3_4:-58381079 Msa0509790:CDS
GATACAAGCAGCAGAAACAT+TGG 0.593081 3_4:+58378917 None:intergenic
TTGTTGAAACAAAGATGGGA+AGG 0.595444 3_4:+58380942 None:intergenic
CTTAACGAGTGCGAAATTGG+AGG 0.610168 3_4:-58378978 Msa0509790:CDS
AAGATGGGAAGGTGTTCTTG+TGG 0.616586 3_4:+58380953 None:intergenic
ATGGAGCGAACGAGCAGTTG+GGG 0.617541 3_4:-58381208 Msa0509790:CDS
ACGAGTGCGAAATTGGAGGA+GGG 0.618522 3_4:-58378974 Msa0509790:CDS
ATTTGTGTACAATCCTGCAA+TGG 0.626316 3_4:-58381017 Msa0509790:CDS
GGGAGCCTAACAACTTTCAG+CGG 0.627758 3_4:-58379627 Msa0509790:CDS
AACCTAAGCCATACACCAAG+AGG 0.629036 3_4:+58379017 None:intergenic
GATCATCATTGGTTTCTGGA+CGG 0.641979 3_4:-58379267 Msa0509790:CDS
GTAGCCGGTTGTTAGACCAA+TGG 0.643579 3_4:+58379653 None:intergenic
GGCTAGATGTTCAGATACGT+AGG 0.652546 3_4:+58379674 None:intergenic
TCACTCACATTCTTGAAACA+GGG 0.660582 3_4:+58378481 None:intergenic
ATATAGTTATTATTAGATGG+CGG 0.673569 3_4:+58380983 None:intergenic
GTGTGTCAAAATGTAGATGT+TGG 0.674681 3_4:-58381163 Msa0509790:CDS
GAAGCTGAGAATCGTGAGTG+TGG 0.689344 3_4:-58381133 Msa0509790:CDS
TAAATATGCAGGTCTTAACG+AGG 0.700815 3_4:-58380354 Msa0509790:intron
TGGTCACTACCAATTGACGA+AGG 0.735981 3_4:+58379153 None:intergenic
GTGGTGGTGAAGCTCAGTTG+TGG 0.736786 3_4:-58379058 Msa0509790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTCGAGTTATTTTT+CGG - chr3_4:58377983-58378002 Msa0509790:three_prime_UTR 15.0%
!!! TGTTATTTAATCTAATCTTT+AGG - chr3_4:58378021-58378040 Msa0509790:three_prime_UTR 15.0%
!! ACAATATAGTTATTATTAGA+TGG + chr3_4:58378402-58378421 None:intergenic 15.0%
!! TATATCATTAACAACAAAAA+AGG + chr3_4:58378516-58378535 None:intergenic 15.0%
!!! TTTATTTCTTTGAATTCTAA+AGG - chr3_4:58379170-58379189 Msa0509790:CDS 15.0%
!! ATAATTCCTAAGATTAAAAA+TGG - chr3_4:58379410-58379429 Msa0509790:intron 15.0%
!! TTTAATAAAATGCATTTGAT+AGG + chr3_4:58379566-58379585 None:intergenic 15.0%
!! TAATATTTCAAAATAGTCTA+TGG - chr3_4:58380582-58380601 Msa0509790:intron 15.0%
!!! TTTTTTTTTTGACACATATT+AGG - chr3_4:58381068-58381087 Msa0509790:CDS 15.0%
!! AAAATGATGTATCATACAAA+AGG + chr3_4:58377891-58377910 None:intergenic 20.0%
!!! TTGCTCTTTTTTATTTTAGA+AGG - chr3_4:58378115-58378134 Msa0509790:intron 20.0%
!!! AAGTTCTTATATATCTTTTG+AGG - chr3_4:58378142-58378161 Msa0509790:three_prime_UTR 20.0%
!! TTATATATCAAACCAGAAAA+TGG - chr3_4:58378336-58378355 Msa0509790:three_prime_UTR 20.0%
!! ATATAGTTATTATTAGATGG+CGG + chr3_4:58378399-58378418 None:intergenic 20.0%
!!! ATGCTTTTACTTTGATTTTT+AGG - chr3_4:58378584-58378603 Msa0509790:CDS 20.0%
!! AAATAGAGAAAAAAAACTCA+AGG + chr3_4:58378719-58378738 None:intergenic 20.0%
!! AATTTCAACAACAAATTAGA+CGG - chr3_4:58378829-58378848 Msa0509790:intron 20.0%
!! ATGTGTTAAATTATGTTTAG+TGG - chr3_4:58378866-58378885 Msa0509790:intron 20.0%
!! TGATTAATGAATATAACTAG+TGG - chr3_4:58378890-58378909 Msa0509790:CDS 20.0%
!! TGCATATTTATTATGACAAT+TGG + chr3_4:58379015-58379034 None:intergenic 20.0%
!! AATTGTCATAATAAATATGC+AGG - chr3_4:58379014-58379033 Msa0509790:CDS 20.0%
!!! ATGTTAGTAAATTTAGAGTA+TGG - chr3_4:58379233-58379252 Msa0509790:CDS 20.0%
!!! ATAGTTCCATTTTTAATCTT+AGG + chr3_4:58379419-58379438 None:intergenic 20.0%
!! GAAATAAATGAGTTCAAAAA+TGG - chr3_4:58380299-58380318 Msa0509790:CDS 20.0%
!! TGTATGATACATCATTTTGT+AGG - chr3_4:58377893-58377912 Msa0509790:three_prime_UTR 25.0%
! ATCACTATGTTTCTTTCTAT+AGG + chr3_4:58378077-58378096 None:intergenic 25.0%
!!! TTACTTTGATTTTTAGGTTC+TGG - chr3_4:58378590-58378609 Msa0509790:CDS 25.0%
! TTTCTTGATGTTAAAGAGTA+TGG - chr3_4:58378747-58378766 Msa0509790:intron 25.0%
! GGAAATATGTTAAATGAGTT+TGG - chr3_4:58378779-58378798 Msa0509790:intron 25.0%
! TACTTATTGCAAAAGTCATT+TGG - chr3_4:58379048-58379067 Msa0509790:CDS 25.0%
!! GAATTCTAAAGGAGAATAAA+AGG - chr3_4:58379181-58379200 Msa0509790:CDS 25.0%
! TAAAAGGAGAAAAATAACCA+TGG - chr3_4:58379197-58379216 Msa0509790:CDS 25.0%
! AAATAACCATGGTAGTTATA+AGG - chr3_4:58379208-58379227 Msa0509790:CDS 25.0%
! TTATCATGATGACTAAATCT+TGG - chr3_4:58379256-58379275 Msa0509790:CDS 25.0%
! AAAGCAATTCAAGTCTTTAT+TGG - chr3_4:58379303-58379322 Msa0509790:intron 25.0%
!!! ACTTCTATTTTTGCTTTCTT+AGG - chr3_4:58379517-58379536 Msa0509790:intron 25.0%
!!! TGGTTTGGTTTTGTTTTTAA+AGG - chr3_4:58379674-58379693 Msa0509790:CDS 25.0%
! CATATTAAGAAGAGTTTGTA+AGG - chr3_4:58380051-58380070 Msa0509790:intron 25.0%
! ATAAATGAGTTCAAAAATGG+TGG - chr3_4:58380302-58380321 Msa0509790:CDS 25.0%
!!! AAAACTTACTGATTTCTTGA+GGG + chr3_4:58380504-58380523 None:intergenic 25.0%
!!! AAAAACTTACTGATTTCTTG+AGG + chr3_4:58380505-58380524 None:intergenic 25.0%
!! GAATTTTTGACACATGATTT+TGG - chr3_4:58380642-58380661 Msa0509790:intron 25.0%
!! ATTGCTATAAACCTGATTTT+TGG - chr3_4:58380679-58380698 Msa0509790:intron 25.0%
! AAAAGAAAATGAAACGCAAA+TGG + chr3_4:58380858-58380877 None:intergenic 25.0%
!! ATTTGCGTTTCATTTTCTTT+TGG - chr3_4:58380857-58380876 Msa0509790:intron 25.0%
!! TTTGCGTTTCATTTTCTTTT+GGG - chr3_4:58380858-58380877 Msa0509790:intron 25.0%
! GATTATGTACCTATTTGTTT+GGG - chr3_4:58380928-58380947 Msa0509790:CDS 25.0%
! TTGTACAATAGTGAATTTGT+TGG - chr3_4:58380998-58381017 Msa0509790:CDS 25.0%
!! TGTACAATAGTGAATTTGTT+GGG - chr3_4:58380999-58381018 Msa0509790:CDS 25.0%
! GTTGGAAGAGATATTATATT+AGG - chr3_4:58381035-58381054 Msa0509790:CDS 25.0%
! TGAACTACAAGATTCTTATT+CGG - chr3_4:58381300-58381319 Msa0509790:five_prime_UTR 25.0%
!!! TTCATAACCTTTTTTCCTAA+GGG + chr3_4:58381368-58381387 None:intergenic 25.0%
! AAAGAAACATTGTTCTTGTT+TGG - chr3_4:58381471-58381490 Msa0509790:five_prime_UTR 25.0%
!! ATTAACTTGCAGCTTAAGTT+AGG - chr3_4:58377946-58377965 Msa0509790:three_prime_UTR 30.0%
!!! TTTTCGAGTTATTTTTCGGA+AGG - chr3_4:58377987-58378006 Msa0509790:three_prime_UTR 30.0%
! CTGGTTTGATATATAAAGAC+AGG + chr3_4:58378332-58378351 None:intergenic 30.0%
! TACACAAATGTTCCATTTTC+TGG + chr3_4:58378351-58378370 None:intergenic 30.0%
! CCATTTGTTGAAACAAAGAT+GGG + chr3_4:58378444-58378463 None:intergenic 30.0%
! TCCATTTGTTGAAACAAAGA+TGG + chr3_4:58378445-58378464 None:intergenic 30.0%
! CTTTGTTTCAACAAATGGAA+AGG - chr3_4:58378446-58378465 Msa0509790:three_prime_UTR 30.0%
TGTGAAGACATAAAACAAGT+AGG - chr3_4:58378474-58378493 Msa0509790:exon 30.0%
! AAATTGTACTAGTTTCCTCA+CGG - chr3_4:58378554-58378573 Msa0509790:CDS 30.0%
ACATAACCTTATAACTACCA+TGG + chr3_4:58379217-58379236 None:intergenic 30.0%
!! ACTTGAATTGCTTTTCAATC+AGG + chr3_4:58379297-58379316 None:intergenic 30.0%
TTGCTTTCTTAGGATCAATA+TGG - chr3_4:58379527-58379546 Msa0509790:intron 30.0%
! CTTTTACACATTTTCCAGAT+TGG - chr3_4:58379635-58379654 Msa0509790:CDS 30.0%
ATAAGTGCGATTTAAAGACT+AGG - chr3_4:58379849-58379868 Msa0509790:intron 30.0%
! TCTAATCTCATTTTACAGCA+TGG + chr3_4:58379998-58380017 None:intergenic 30.0%
AAGAAGAGTTTGTAAGGAAA+TGG - chr3_4:58380057-58380076 Msa0509790:intron 30.0%
! GTAAGGAAATGGAATTTTGT+AGG - chr3_4:58380068-58380087 Msa0509790:intron 30.0%
! CTTTTATATCGAAACTAAGC+TGG - chr3_4:58380189-58380208 Msa0509790:intron 30.0%
TAATATCACCAACAACACTA+TGG + chr3_4:58380249-58380268 None:intergenic 30.0%
!! TGGTGATATTAGCTTTGTTA+TGG - chr3_4:58380258-58380277 Msa0509790:intron 30.0%
!! GGTGATATTAGCTTTGTTAT+GGG - chr3_4:58380259-58380278 Msa0509790:intron 30.0%
!! GTGATATTAGCTTTGTTATG+GGG - chr3_4:58380260-58380279 Msa0509790:intron 30.0%
!!! TTTTTGAAATGGCTTCGATT+TGG - chr3_4:58380428-58380447 Msa0509790:intron 30.0%
!!! TTTTGAAATGGCTTCGATTT+GGG - chr3_4:58380429-58380448 Msa0509790:intron 30.0%
! TTTTTGACACATGATTTTGG+AGG - chr3_4:58380645-58380664 Msa0509790:intron 30.0%
AATTGTGATACTGTGATAAC+AGG - chr3_4:58380719-58380738 Msa0509790:CDS 30.0%
TGATAGAAAACAAGCATCTT+TGG - chr3_4:58380807-58380826 Msa0509790:intron 30.0%
GGATTATGTACCTATTTGTT+TGG - chr3_4:58380927-58380946 Msa0509790:CDS 30.0%
TTTCAATACATTCTTCCCTT+AGG - chr3_4:58381350-58381369 Msa0509790:five_prime_UTR 30.0%
!!! GTTCATAACCTTTTTTCCTA+AGG + chr3_4:58381369-58381388 None:intergenic 30.0%
!!! TTTCTCTAGCTTTTTGATGA+GGG - chr3_4:58381516-58381535 Msa0509790:five_prime_UTR 30.0%
CAGCTTAAGTTAGGTATAAC+AGG - chr3_4:58377955-58377974 Msa0509790:three_prime_UTR 35.0%
!! ATATCTTTTGAGGATTGCCA+TGG - chr3_4:58378152-58378171 Msa0509790:three_prime_UTR 35.0%
GTGTGTCAAAATGTAGATGT+TGG - chr3_4:58378216-58378235 Msa0509790:three_prime_UTR 35.0%
!! ATATAGCTTTTCAAGGAGGA+AGG - chr3_4:58378283-58378302 Msa0509790:three_prime_UTR 35.0%
ATTTGTGTACAATCCTGCAA+TGG - chr3_4:58378362-58378381 Msa0509790:three_prime_UTR 35.0%
TTGTTGAAACAAAGATGGGA+AGG + chr3_4:58378440-58378459 None:intergenic 35.0%
! CCCATCTTTGTTTCAACAAA+TGG - chr3_4:58378441-58378460 Msa0509790:three_prime_UTR 35.0%
GAAACAAATTCCAGTAGCTT+GGG + chr3_4:58378633-58378652 None:intergenic 35.0%
TGTTTCGTGTCTAACTCATT+TGG - chr3_4:58378647-58378666 Msa0509790:CDS 35.0%
!! CTAACTCATTTGGCTGTTTT+TGG - chr3_4:58378657-58378676 Msa0509790:CDS 35.0%
! TAAAGAGTATGGTAGATGCT+AGG - chr3_4:58378758-58378777 Msa0509790:intron 35.0%
CTACTCGACGCAATTAAATT+AGG - chr3_4:58378953-58378972 Msa0509790:CDS 35.0%
TAAATATGCAGGTCTTAACG+AGG - chr3_4:58379025-58379044 Msa0509790:CDS 35.0%
GTAAAAAACGGTATGATCAC+TGG + chr3_4:58379094-58379113 None:intergenic 35.0%
TGGCAGATCAAGGTAAAAAA+CGG + chr3_4:58379106-58379125 None:intergenic 35.0%
TAAGAATCAGTGAATGCAGA+AGG + chr3_4:58379148-58379167 None:intergenic 35.0%
GCTGCATTAACTAACATGTT+TGG - chr3_4:58379330-58379349 Msa0509790:intron 35.0%
AAAATGGAACTATCTGTTCC+AGG - chr3_4:58379426-58379445 Msa0509790:intron 35.0%
!! CAGATTGGTTCCTTTTTGAT+GGG - chr3_4:58379650-58379669 Msa0509790:CDS 35.0%
TATATCGAAACTAAGCTGGA+AGG - chr3_4:58380193-58380212 Msa0509790:intron 35.0%
AATGAGTTCAAAAATGGTGG+TGG - chr3_4:58380305-58380324 Msa0509790:CDS 35.0%
!!! ATAAACCTGATTTTTGGTGC+AGG - chr3_4:58380685-58380704 Msa0509790:intron 35.0%
GTGATAACAGGACTTCAATT+TGG - chr3_4:58380731-58380750 Msa0509790:CDS 35.0%
AGGACTTCAATTTGGTCTAT+TGG - chr3_4:58380739-58380758 Msa0509790:CDS 35.0%
GGACTTCAATTTGGTCTATT+GGG - chr3_4:58380740-58380759 Msa0509790:CDS 35.0%
TCACTCACATTCTTGAAACA+GGG + chr3_4:58380901-58380920 None:intergenic 35.0%
TACCTATTTGTTTGGGTGTT+TGG - chr3_4:58380935-58380954 Msa0509790:CDS 35.0%
!! AAGTTTTATCATGTGTGCTC+TGG - chr3_4:58380975-58380994 Msa0509790:CDS 35.0%
ACACATATTAGGATCTTCGT+GGG - chr3_4:58381079-58381098 Msa0509790:CDS 35.0%
!! ATCAATTGTTTTGCATTGCG+CGG - chr3_4:58381234-58381253 Msa0509790:five_prime_UTR 35.0%
ACTACAAGATTCTTATTCGG+TGG - chr3_4:58381303-58381322 Msa0509790:five_prime_UTR 35.0%
CTACAAGATTCTTATTCGGT+GGG - chr3_4:58381304-58381323 Msa0509790:five_prime_UTR 35.0%
CATTCTTCCCTTAGGAAAAA+AGG - chr3_4:58381358-58381377 Msa0509790:five_prime_UTR 35.0%
GGAAAAAAGGTTATGAACGT+TGG - chr3_4:58381371-58381390 Msa0509790:five_prime_UTR 35.0%
!!! CTTTCTCTAGCTTTTTGATG+AGG - chr3_4:58381515-58381534 Msa0509790:five_prime_UTR 35.0%
! GCAGGAGATATAGCTTTTCA+AGG - chr3_4:58378276-58378295 Msa0509790:three_prime_UTR 40.0%
!! GGAGATATAGCTTTTCAAGG+AGG - chr3_4:58378279-58378298 Msa0509790:three_prime_UTR 40.0%
CGAAACAAATTCCAGTAGCT+TGG + chr3_4:58378634-58378653 None:intergenic 40.0%
!!! TTGGCTGTTTTTGGCATTCT+TGG - chr3_4:58378666-58378685 Msa0509790:CDS 40.0%
!!! TGGCTGTTTTTGGCATTCTT+GGG - chr3_4:58378667-58378686 Msa0509790:CDS 40.0%
TGCGTCGAGTAGATGATTAA+TGG + chr3_4:58378945-58378964 None:intergenic 40.0%
CGACGCAATTAAATTAGGAG+AGG - chr3_4:58378958-58378977 Msa0509790:CDS 40.0%
CAAAAGTCATTTGGACCAAG+TGG - chr3_4:58379057-58379076 Msa0509790:CDS 40.0%
! TCATTTGGACCAAGTGGTTT+TGG - chr3_4:58379063-58379082 Msa0509790:CDS 40.0%
! CCATATTTGATGGCAGATCA+AGG + chr3_4:58379116-58379135 None:intergenic 40.0%
CCTTGATCTGCCATCAAATA+TGG - chr3_4:58379113-58379132 Msa0509790:CDS 40.0%
AATGCTTCAAGCTCAAAACC+TGG + chr3_4:58379447-58379466 None:intergenic 40.0%
CCATCAAAAAGGAACCAATC+TGG + chr3_4:58379652-58379671 None:intergenic 40.0%
!! CCAGATTGGTTCCTTTTTGA+TGG - chr3_4:58379649-58379668 Msa0509790:CDS 40.0%
! TTGGTTCCTTTTTGATGGGA+TGG - chr3_4:58379654-58379673 Msa0509790:CDS 40.0%
! TCCTTTTTGATGGGATGGTT+TGG - chr3_4:58379659-58379678 Msa0509790:CDS 40.0%
ACCAAACCATCCCATCAAAA+AGG + chr3_4:58379663-58379682 None:intergenic 40.0%
GTATCTGAACATCTAGCCAT+TGG - chr3_4:58379710-58379729 Msa0509790:CDS 40.0%
GTAGCATTGTCGATCATCAT+TGG - chr3_4:58380101-58380120 Msa0509790:intron 40.0%
!! TGTCGATCATCATTGGTTTC+TGG - chr3_4:58380108-58380127 Msa0509790:intron 40.0%
!! GATCATCATTGGTTTCTGGA+CGG - chr3_4:58380112-58380131 Msa0509790:intron 40.0%
AGACTTAACGAGTGCGAAAT+TGG - chr3_4:58380398-58380417 Msa0509790:intron 40.0%
!! TTGGAGGAGGGTTTTTGAAA+TGG - chr3_4:58380417-58380436 Msa0509790:intron 40.0%
GATACAAGCAGCAGAAACAT+TGG + chr3_4:58380465-58380484 None:intergenic 40.0%
! TGTTTCTGCTGCTTGTATCA+CGG - chr3_4:58380466-58380485 Msa0509790:intron 40.0%
!! ACTTACTGATTTCTTGAGGG+TGG + chr3_4:58380501-58380520 None:intergenic 40.0%
ATTCACCTGCACCAAAAATC+AGG + chr3_4:58380693-58380712 None:intergenic 40.0%
!! GAGAGCATTTGTCTATGCTA+TGG - chr3_4:58380829-58380848 Msa0509790:intron 40.0%
CTCACTCACATTCTTGAAAC+AGG + chr3_4:58380902-58380921 None:intergenic 40.0%
TCAAGAATGTGAGTGAGCAT+AGG - chr3_4:58380905-58380924 Msa0509790:intron 40.0%
CAAGAATGTGAGTGAGCATA+GGG - chr3_4:58380906-58380925 Msa0509790:intron 40.0%
AGCCAAACACCCAAACAAAT+AGG + chr3_4:58380940-58380959 None:intergenic 40.0%
!! GAATTTGTTGGGTCTGAGTT+AGG - chr3_4:58381010-58381029 Msa0509790:CDS 40.0%
GACACATATTAGGATCTTCG+TGG - chr3_4:58381078-58381097 Msa0509790:CDS 40.0%
ATTAGGATCTTCGTGGGATT+TGG - chr3_4:58381085-58381104 Msa0509790:CDS 40.0%
!!! TCTAGCTTTTTGATGAGGGT+GGG - chr3_4:58381520-58381539 Msa0509790:five_prime_UTR 40.0%
GGCAATTCATGTTCCATTGC+AGG + chr3_4:58378378-58378397 None:intergenic 45.0%
!! AAGATGGGAAGGTGTTCTTG+TGG + chr3_4:58378429-58378448 None:intergenic 45.0%
! TACTAGTTTCCTCACGGTTG+AGG - chr3_4:58378560-58378579 Msa0509790:CDS 45.0%
CATGAAGTTACCCAAGCTAC+TGG - chr3_4:58378620-58378639 Msa0509790:CDS 45.0%
!!! GGCTGTTTTTGGCATTCTTG+GGG - chr3_4:58378668-58378687 Msa0509790:CDS 45.0%
ATCAGTGAATGCAGAAGGAG+AGG + chr3_4:58379143-58379162 None:intergenic 45.0%
GGCTAGATGTTCAGATACGT+AGG + chr3_4:58379708-58379727 None:intergenic 45.0%
! CTAGCCATTGGTCTAACAAC+CGG - chr3_4:58379722-58379741 Msa0509790:CDS 45.0%
GTCTAACAACCGGCTACTTA+GGG - chr3_4:58379732-58379751 Msa0509790:intron 45.0%
CACTGGATCTTGTCTTTGCT+CGG - chr3_4:58379812-58379831 Msa0509790:intron 45.0%
! TCTTTGCTCGGCTTTGTACT+AGG - chr3_4:58379824-58379843 Msa0509790:intron 45.0%
TGACAGCTTCCTTCGTCAAT+TGG - chr3_4:58380217-58380236 Msa0509790:intron 45.0%
TGGTCACTACCAATTGACGA+AGG + chr3_4:58380229-58380248 None:intergenic 45.0%
GGTAGTGACCATAGTGTTGT+TGG - chr3_4:58380238-58380257 Msa0509790:intron 45.0%
!! TTGTGGTTAGCCTGCATTGT+TGG - chr3_4:58380338-58380357 Msa0509790:CDS 45.0%
! TTGTTGGACCTCTTGGTGTA+TGG - chr3_4:58380354-58380373 Msa0509790:intron 45.0%
AACCTAAGCCATACACCAAG+AGG + chr3_4:58380365-58380384 None:intergenic 45.0%
CTTAACGAGTGCGAAATTGG+AGG - chr3_4:58380401-58380420 Msa0509790:intron 45.0%
TTGGGTCTGAGTTAGGAAGT+TGG - chr3_4:58381017-58381036 Msa0509790:CDS 45.0%
!!! CTCTAGCTTTTTGATGAGGG+TGG - chr3_4:58381519-58381538 Msa0509790:five_prime_UTR 45.0%
GAAGCTGAGAATCGTGAGTG+TGG - chr3_4:58378246-58378265 Msa0509790:three_prime_UTR 50.0%
GGAGGAAGGAAAAGTGAGAG+TGG - chr3_4:58378297-58378316 Msa0509790:three_prime_UTR 50.0%
GGAAGGAAAAGTGAGAGTGG+TGG - chr3_4:58378300-58378319 Msa0509790:three_prime_UTR 50.0%
! AAAAGCATGCCTCAACCGTG+AGG + chr3_4:58378572-58378591 None:intergenic 50.0%
GAGAGGCGGACCATATTTGA+TGG + chr3_4:58379126-58379145 None:intergenic 50.0%
AGTGAATGCAGAAGGAGAGG+CGG + chr3_4:58379140-58379159 None:intergenic 50.0%
GTAGCCGGTTGTTAGACCAA+TGG + chr3_4:58379729-58379748 None:intergenic 50.0%
GGTCTAACAACCGGCTACTT+AGG - chr3_4:58379731-58379750 Msa0509790:intron 50.0%
TGTTAGGCTCCCTAAGTAGC+CGG + chr3_4:58379744-58379763 None:intergenic 50.0%
GGGAGCCTAACAACTTTCAG+CGG - chr3_4:58379752-58379771 Msa0509790:intron 50.0%
TCCAGCCGCTGAAAGTTGTT+AGG + chr3_4:58379760-58379779 None:intergenic 50.0%
! ATTGGTTTCTGGACGGCGAA+GGG - chr3_4:58380119-58380138 Msa0509790:intron 50.0%
ATGGGGTGTGAGTAGTGCTT+TGG - chr3_4:58380277-58380296 Msa0509790:CDS 50.0%
ACCAAGAGGTCCAACAATGC+AGG + chr3_4:58380351-58380370 None:intergenic 50.0%
! GACCTCTTGGTGTATGGCTT+AGG - chr3_4:58380360-58380379 Msa0509790:intron 50.0%
AACGAGTGCGAAATTGGAGG+AGG - chr3_4:58380404-58380423 Msa0509790:intron 50.0%
ACGAGTGCGAAATTGGAGGA+GGG - chr3_4:58380405-58380424 Msa0509790:intron 50.0%
CATGGAGCGAACGAGCAGTT+GGG - chr3_4:58378170-58378189 Msa0509790:three_prime_UTR 55.0%
ATGGAGCGAACGAGCAGTTG+GGG - chr3_4:58378171-58378190 Msa0509790:three_prime_UTR 55.0%
GTTGGGGTTCTGCAGTTAGC+AGG - chr3_4:58378187-58378206 Msa0509790:three_prime_UTR 55.0%
CTGGTCACGCCAAAACCACT+TGG + chr3_4:58379075-58379094 None:intergenic 55.0%
GCCTAACAACTTTCAGCGGC+TGG - chr3_4:58379756-58379775 Msa0509790:intron 55.0%
!! CATTGGTTTCTGGACGGCGA+AGG - chr3_4:58380118-58380137 Msa0509790:intron 55.0%
GGACGGCGAAGGGATTCAAA+TGG - chr3_4:58380129-58380148 Msa0509790:intron 55.0%
! GTGGTGGTGAAGCTCAGTTG+TGG - chr3_4:58380321-58380340 Msa0509790:CDS 55.0%
GCCTGCATTGTTGGACCTCT+TGG - chr3_4:58380347-58380366 Msa0509790:intron 55.0%
CCAACTGCTCGTTCGCTCCA+TGG + chr3_4:58378172-58378191 None:intergenic 60.0%
CCATGGAGCGAACGAGCAGT+TGG - chr3_4:58378169-58378188 Msa0509790:three_prime_UTR 60.0%
CGTGAGTGTGGTTCAGCTGC+AGG - chr3_4:58378258-58378277 Msa0509790:three_prime_UTR 60.0%
ATGCTCGAGCTCAGTGTCGC+TGG - chr3_4:58379788-58379807 Msa0509790:intron 60.0%
! AGCTCAGTGTCGCTGGACAC+TGG - chr3_4:58379795-58379814 Msa0509790:intron 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 58377821 58381580 58377821 ID=Msa0509790;Name=Msa0509790
chr3_4 mRNA 58377821 58381580 58377821 ID=Msa0509790-mRNA-1;Parent=Msa0509790;Name=Msa0509790-mRNA-1;_AED=0.30;_eAED=0.30;_QI=350|1|1|1|0.83|0.71|7|642|443
chr3_4 exon 58377821 58378113 58377821 ID=Msa0509790-mRNA-1:exon:13525;Parent=Msa0509790-mRNA-1
chr3_4 exon 58378136 58378694 58378136 ID=Msa0509790-mRNA-1:exon:13524;Parent=Msa0509790-mRNA-1
chr3_4 exon 58378887 58379311 58378887 ID=Msa0509790-mRNA-1:exon:13523;Parent=Msa0509790-mRNA-1
chr3_4 exon 58379556 58379748 58379556 ID=Msa0509790-mRNA-1:exon:13522;Parent=Msa0509790-mRNA-1
chr3_4 exon 58380267 58380365 58380267 ID=Msa0509790-mRNA-1:exon:13521;Parent=Msa0509790-mRNA-1
chr3_4 exon 58380712 58380795 58380712 ID=Msa0509790-mRNA-1:exon:13520;Parent=Msa0509790-mRNA-1
chr3_4 exon 58380910 58381580 58380910 ID=Msa0509790-mRNA-1:exon:13519;Parent=Msa0509790-mRNA-1
chr3_4 five_prime_UTR 58381231 58381580 58381231 ID=Msa0509790-mRNA-1:five_prime_utr;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58380910 58381230 58380910 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58380712 58380795 58380712 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58380267 58380365 58380267 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58379556 58379748 58379556 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58378887 58379311 58378887 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 CDS 58378485 58378694 58378485 ID=Msa0509790-mRNA-1:cds;Parent=Msa0509790-mRNA-1
chr3_4 three_prime_UTR 58378136 58378484 58378136 ID=Msa0509790-mRNA-1:three_prime_utr;Parent=Msa0509790-mRNA-1
chr3_4 three_prime_UTR 58377821 58378113 58377821 ID=Msa0509790-mRNA-1:three_prime_utr;Parent=Msa0509790-mRNA-1
Gene Sequence

>Msa0509790

ATGGAGCGAACGAGCAGTTGGGGTTCTGCAGTTAGCAGGCTTTCAGTGTGTCAAAATGTAGATGTTGGTGATGACGAAGCTGAGAATCGTGAGTGTGGTTCAGCTGCAGGAGATATAGCTTTTCAAGGAGGAAGGAAAAGTGAGAGTGGTGGCTTTGACCTGTCTTTATATATCAAACCAGAAAATGGAACATTTGTGTACAATCCTGCAATGGAACATGAATTGCCGCCATCTAATAATAACTATATTGTAAATCCACAAGAACACCTTCCCATCTTTGTTTCAACAAATGGAAAGGTTAATTGTGAAGACATAAAACAAGTTCTGGAACTTGACATGAAGTTACCCAAGCTACTGGAATTTGTTTCGTGTCTAACTCATTTGGCTGTTTTTGGCATTCTTGGGGTCTTAACGAGGTACTTATTGCAAAAGTCATTTGGACCAAGTGGTTTTGGCGTGACCAGTGATCATACCGTTTTTTACCTTGATCTGCCATCAAATATGATTGGTTCCTTTTTGATGGGATGGTTTGGTTTTGTTTTTAAAGGAGATATATCCTACGTATCTGAACATCTAGCCATTGGTCTAACAACCGGCTACTTAGGGAGCCTAACAACTTTCAGCGGCTGGAATCAGAAAATGCTCGAGCTCAGTGTCGCTGGACACTGGATCTTGTCTTTGCTCGGCTTTGTACTAGGCATGTTTCTTGTAGCATTGTCGATCATCATTGGTTTCTGGACGGCGAAGGGATTCAAATGGTTACTCAAAAGACTAAACATGAGACATGAAAGTGACTCTTTTATATCGAAACTAAGCTGGAAGGTTGACAGCTTCCTTCGTCAATTGGTAGTGACCATAGTGTTGTTGGTGATATTAGCTTTGTTATGGGGTGTGAGTAGTGCTTTGGAAATAAATGAGTTCAAAAATGGTGGTGGTGAAGCTCAGTTGTGGTTAGCCTGCATTGTTGGACCTCTTGGTGTATGGCTTAGGTTTTTCTTAGCTACTAGACTTAACGAGTGCGAAATTGGAGGAGGGTTTTTGAAATGGCTTCGATTTGGGACTCTTATTGCCAATGTTTCTGCTGCTTGTATCACGGCTGCACTTGCCACCCTCAAGAAATCAGTGAATACTACAAATTGTGATACTGTGATAACAGGACTTCAATTTGGTCTATTGGGATGTCTGAGTACTGTTTCAACATTTGTTGCTGAATTGTATGAAATGATAGAAAACAAGCATCTTTGGAGAGCATTTGTCTATGCTATGGTTACCATTTGCGTTTCATTTTCTTTTGGGATCTTATTATACTATATCCCTGTTTCAAGAATGTGA
Protein sequence

>Msa0509790

MERTSSWGSAVSRLSVCQNVDVGDDEAENRECGSAAGDIAFQGGRKSESGGFDLSLYIKPENGTFVYNPAMEHELPPSNNNYIVNPQEHLPIFVSTNGKVNCEDIKQVLELDMKLPKLLEFVSCLTHLAVFGILGVLTRYLLQKSFGPSGFGVTSDHTVFYLDLPSNMIGSFLMGWFGFVFKGDISYVSEHLAIGLTTGYLGSLTTFSGWNQKMLELSVAGHWILSLLGFVLGMFLVALSIIIGFWTAKGFKWLLKRLNMRHESDSFISKLSWKVDSFLRQLVVTIVLLVILALLWGVSSALEINEFKNGGGEAQLWLACIVGPLGVWLRFFLATRLNECEIGGGFLKWLRFGTLIANVSAACITAALATLKKSVNTTNCDTVITGLQFGLLGCLSTVSTFVAELYEMIENKHLWRAFVYAMVTICVSFSFGILLYYIPVSRM*