AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0370030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0370030 MtrunA17_Chr3g0114931 92.135 445 27 3 1 443 1 439 0.0 794
Msa0370030 MtrunA17_Chr3g0114921 54.566 449 186 9 1 439 1 441 1.15e-152 441
Msa0370030 MtrunA17_Chr3g0078481 64.470 349 119 4 96 443 36 380 4.92e-144 416
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0370030 AT2G41705.3 51.544 421 189 7 29 440 41 455 1.14e-119 357
Msa0370030 AT2G41705.2 51.544 421 189 7 29 440 41 455 1.14e-119 357
Msa0370030 AT2G41705.4 51.544 421 189 7 29 440 41 455 1.14e-119 357
Msa0370030 AT2G41705.1 51.544 421 189 7 29 440 41 455 1.14e-119 357

Find 109 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGATCTGCCATCAAATA+TGG 0.217091 3_1:-67434788 Msa0370030:intron
AGGACTTCAATTTGGTCTAT+TGG 0.243981 3_1:-67433189 Msa0370030:CDS
TGTCGATCATCATTGGTTTC+TGG 0.244597 3_1:-67433820 Msa0370030:CDS
ATTAGGATCTTCGTGGGATT+TGG 0.260829 3_1:-67432840 Msa0370030:three_prime_UTR
TACCTATTTGTTTGGGTGTT+TGG 0.268786 3_1:-67432993 Msa0370030:three_prime_UTR
TGGTGATATTAGCTTTGTTA+TGG 0.283155 3_1:-67433670 Msa0370030:CDS
CCCATCTTTGTTTCAACAAA+TGG 0.286309 3_1:-67435461 Msa0370030:CDS
GACCTCTTGGTGTATGGATT+AGG 0.291990 3_1:-67433568 Msa0370030:CDS
GCCTGCATTGTTGGACCTCT+TGG 0.293298 3_1:-67433581 Msa0370030:CDS
GTTGGAAGAGATATTATATT+AGG 0.293744 3_1:-67432893 Msa0370030:three_prime_UTR
AGACTTAACGAGTGCGAAAT+TGG 0.297010 3_1:-67433530 Msa0370030:CDS
TGTTATTTAATCTAATCTTT+AGG 0.299381 3_1:-67435845 Msa0370030:five_prime_UTR
TCATTTGGACCAAGTGGTTT+TGG 0.299453 3_1:-67434838 Msa0370030:CDS
GGATTATGTACCTATTTGTT+TGG 0.307962 3_1:-67433001 Msa0370030:three_prime_UTR
TGATAGAAAACAAGCATCTT+TGG 0.308824 3_1:-67433121 Msa0370030:CDS
AAAGAAACATTGTTCTTGTT+TGG 0.311813 3_1:-67432481 Msa0370030:three_prime_UTR
GGACTTCAATTTGGTCTATT+GGG 0.318154 3_1:-67433188 Msa0370030:CDS
CTCACTCACATTCTTGAAAC+AGG 0.318173 3_1:+67433029 None:intergenic
ACAATATAGTTATTATTAGA+TGG 0.318561 3_1:+67435503 None:intergenic
TTATATATCAAACCAGAAAA+TGG 0.319807 3_1:-67435566 Msa0370030:CDS
GTGATAACAGGACTTCAATT+TGG 0.320323 3_1:-67433197 Msa0370030:CDS
TGTTGATCTTTGCTCTTTCA+AGG 0.329769 3_1:-67435760 Msa0370030:five_prime_UTR
ACCAAACCATCCCATCAAAA+AGG 0.332116 3_1:+67434241 None:intergenic
TACTTATTGCAAAAGTCATT+TGG 0.339763 3_1:-67434853 Msa0370030:CDS
GGTGATATTAGCTTTGTTAT+GGG 0.342133 3_1:-67433669 Msa0370030:CDS
GATTATGTACCTATTTGTTT+GGG 0.342788 3_1:-67433000 Msa0370030:three_prime_UTR
GGCAATTCATGTTCCATTGC+AGG 0.342935 3_1:+67435527 None:intergenic
CACTGGATCTTGTCTTTGCT+CGG 0.345396 3_1:-67434089 Msa0370030:CDS
ATCACTATGTTTCTTTCTAT+AGG 0.370649 3_1:+67435792 None:intergenic
TGTTTCGTGTCTAACTCATT+TGG 0.377439 3_1:-67435255 Msa0370030:CDS
TGAACTGCAAGATTCTTATT+CGG 0.379323 3_1:-67432649 Msa0370030:three_prime_UTR
TTGTTGGACCTCTTGGTGTA+TGG 0.382011 3_1:-67433574 Msa0370030:CDS
GAATTTGTTGGGTCTGAGTT+AGG 0.382110 3_1:-67432918 Msa0370030:three_prime_UTR
TCCATTTGTTGAAACAAAGA+TGG 0.386494 3_1:+67435460 None:intergenic
GGTCTAACAACCGGCTACTT+AGG 0.389772 3_1:-67434170 Msa0370030:CDS
TCTTTGCTCGGCTTTGTACT+AGG 0.391256 3_1:-67434077 Msa0370030:intron
AATTGTGATACTGTGATAAC+AGG 0.402053 3_1:-67433209 Msa0370030:CDS
GTTGGGGTTCTGCAGTTAGT+AGG 0.420423 3_1:-67435715 Msa0370030:CDS
AGCCAAACACCCAAACAAAT+AGG 0.425540 3_1:+67432991 None:intergenic
TGACAGCTTCCTTCGTCAAT+TGG 0.430603 3_1:-67433711 Msa0370030:CDS
AAAACTTACTGATTTCTTGA+GGG 0.431062 3_1:+67433427 None:intergenic
CGTGAGTGTGGTTCAGCTGC+AGG 0.439210 3_1:-67435644 Msa0370030:CDS
CATGGAGCGAACGAGCAGTT+GGG 0.449685 3_1:-67435732 Msa0370030:CDS
GCCTAACAACTTTCAGCGGC+TGG 0.451556 3_1:-67434145 Msa0370030:CDS
TCCAGCCGCTGAAAGTTGTT+AGG 0.453775 3_1:+67434144 None:intergenic
CTGGTTTGATATATAAAGAC+AGG 0.458399 3_1:+67435573 None:intergenic
GTAGCATTGTCGATCATCAT+TGG 0.460693 3_1:-67433827 Msa0370030:CDS
TTGTGGTTAGCCTGCATTGT+TGG 0.460887 3_1:-67433590 Msa0370030:CDS
ATGCTCGAGCTCAGTGTCGC+TGG 0.460917 3_1:-67434113 Msa0370030:CDS
CGAAACAAATTCCAGTAGCT+TGG 0.472897 3_1:+67435271 None:intergenic
GTCTAACAACCGGCTACTTA+GGG 0.474314 3_1:-67434169 Msa0370030:CDS
CATGAAATTACCCAAGCTAC+TGG 0.475903 3_1:-67435282 Msa0370030:CDS
CTAGCCATTGGTCTAACAAC+CGG 0.476478 3_1:-67434179 Msa0370030:CDS
GAGAGGCGAACCATATTTGA+TGG 0.476823 3_1:+67434778 None:intergenic
CCATTTGTTGAAACAAAGAT+GGG 0.480162 3_1:+67435461 None:intergenic
AGCTCAGTGTCGCTGGACAC+TGG 0.485522 3_1:-67434106 Msa0370030:CDS
ATGGGGTGTGAGTAGTGCTT+TGG 0.485675 3_1:-67433651 Msa0370030:CDS
TGTACAATAGTGAATTTGTT+GGG 0.492567 3_1:-67432929 Msa0370030:three_prime_UTR
TTGGGTCTGAGTTAGGAAGT+TGG 0.493066 3_1:-67432911 Msa0370030:three_prime_UTR
GAGAGCATTTGTCTATGCTA+TGG 0.494452 3_1:-67433099 Msa0370030:CDS
CAAGAATGTGAGTGAGCATA+GGG 0.494592 3_1:-67433022 Msa0370030:exon
GGTAGTGATCATAGTGTTGT+TGG 0.497849 3_1:-67433690 Msa0370030:CDS
AAAAGAAAATGAAACGCAAA+TGG 0.498184 3_1:+67433073 None:intergenic
CCAACTGCTCGTTCGCTCCA+TGG 0.502807 3_1:+67435733 None:intergenic
GAAACAAATTCCAGTAGCTT+GGG 0.506298 3_1:+67435272 None:intergenic
CCATGGAGCGAACGAGCAGT+TGG 0.508369 3_1:-67435733 Msa0370030:CDS
CTTTGTTTCAACAAATGGAA+AGG 0.511711 3_1:-67435456 Msa0370030:CDS
CATTGGTTTCTGGACGGCGA+AGG 0.512919 3_1:-67433810 Msa0370030:CDS
TGCTCTTTCAAGGATCGCCA+TGG 0.515990 3_1:-67435750 Msa0370030:exon
GTGATATTAGCTTTGTTATG+GGG 0.520721 3_1:-67433668 Msa0370030:CDS
AACGAGTGCGAAATTGGAGG+AGG 0.526946 3_1:-67433524 Msa0370030:CDS
GGAGGAAGGAAAAGTGAGAG+TGG 0.527956 3_1:-67435605 Msa0370030:CDS
TTGTACAATAGTGAATTTGT+TGG 0.531782 3_1:-67432930 Msa0370030:three_prime_UTR
CCATATTTGATGGCAGATCA+AGG 0.532230 3_1:+67434788 None:intergenic
GTATCTGAACATCTAGCCAT+TGG 0.532584 3_1:-67434191 Msa0370030:CDS
GACACATATTAGGATCTTCG+TGG 0.534243 3_1:-67432847 Msa0370030:three_prime_UTR
ATTGGTTTCTGGACGGCGAA+GGG 0.538719 3_1:-67433809 Msa0370030:CDS
TGTTTCTGCTGCTTGTATCA+CGG 0.540208 3_1:-67433462 Msa0370030:CDS
GGACGGCGAAGGGATTCAAA+TGG 0.542568 3_1:-67433799 Msa0370030:CDS
TCAAGAATGTGAGTGAGCAT+AGG 0.546189 3_1:-67433023 Msa0370030:exon
ACCAAGAGGTCCAACAATGC+AGG 0.546644 3_1:+67433580 None:intergenic
CTGGTCACGCCAAAACCACT+TGG 0.550500 3_1:+67434829 None:intergenic
CAAAAGTCATTTGGACCAAG+TGG 0.552223 3_1:-67434844 Msa0370030:CDS
ACTTACTGATTTCTTGAGGG+TGG 0.554227 3_1:+67433430 None:intergenic
CTGCAAGATTCTTATTCGGT+GGG 0.555202 3_1:-67432645 Msa0370030:three_prime_UTR
TGTTAGGCTCCCTAAGTAGC+CGG 0.558390 3_1:+67434160 None:intergenic
GGCTAGATGTTCAGATACAT+AGG 0.562753 3_1:+67434196 None:intergenic
TATATCGAAACTAAGCTGGA+AGG 0.568673 3_1:-67433735 Msa0370030:CDS
CAAATATTTCGCATTTGACA+CGG 0.574633 3_1:+67432670 None:intergenic
GGAAGGAAAAGTGAGAGTGG+TGG 0.583294 3_1:-67435602 Msa0370030:CDS
ACTGCAAGATTCTTATTCGG+TGG 0.587886 3_1:-67432646 Msa0370030:three_prime_UTR
ACACATATTAGGATCTTCGT+GGG 0.592414 3_1:-67432846 Msa0370030:three_prime_UTR
GATACAAGCAGCAGAAACAT+TGG 0.593081 3_1:+67433466 None:intergenic
AACCTAATCCATACACCAAG+AGG 0.609347 3_1:+67433566 None:intergenic
CTTAACGAGTGCGAAATTGG+AGG 0.610168 3_1:-67433527 Msa0370030:CDS
ACGAGTGCGAAATTGGAGGA+GGG 0.618522 3_1:-67433523 Msa0370030:CDS
AAGATGGGAATGTGTTCTTG+TGG 0.620426 3_1:+67435476 None:intergenic
ATTTGTGTACAATCCTGCAA+TGG 0.626316 3_1:-67435540 Msa0370030:CDS
GGGAGCCTAACAACTTTCAG+CGG 0.627758 3_1:-67434149 Msa0370030:CDS
ATGGAGCGAACGAGCAGTTG+GGG 0.627866 3_1:-67435731 Msa0370030:CDS
GAAGCTGAGAATCGTGAGTG+TGG 0.628381 3_1:-67435656 Msa0370030:CDS
GATCATCATTGGTTTCTGGA+CGG 0.641979 3_1:-67433816 Msa0370030:CDS
GTAGCCGGTTGTTAGACCAA+TGG 0.643579 3_1:+67434175 None:intergenic
TCACTCACATTCTTGAAACA+GGG 0.660582 3_1:+67433030 None:intergenic
ATATAGTTATTATTAGATGG+CGG 0.673569 3_1:+67435506 None:intergenic
GTGTGTCAAAATGTAGATGT+TGG 0.674681 3_1:-67435686 Msa0370030:CDS
TAAATATGCAGGTCTTAACG+AGG 0.700815 3_1:-67434876 Msa0370030:intron
TGATCACTACCAATTGACGA+AGG 0.724697 3_1:+67433702 None:intergenic
GTGGTGGTGAAGCTCAGTTG+TGG 0.736786 3_1:-67433607 Msa0370030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTCGAGTTATTTTT+CGG - chr3_1:67432393-67432412 Msa0370030:three_prime_UTR 15.0%
!!! TGTTATTTAATCTAATCTTT+AGG - chr3_1:67432431-67432450 Msa0370030:three_prime_UTR 15.0%
!! ACAATATAGTTATTATTAGA+TGG + chr3_1:67432776-67432795 None:intergenic 15.0%
!! TATATCATTAACAACAAAAA+AGG + chr3_1:67432890-67432909 None:intergenic 15.0%
!!! TTTATTTCTTTGAATTCTAA+AGG - chr3_1:67433545-67433564 Msa0370030:CDS 15.0%
!! ATAATTCCTAAGATTAAAAA+TGG - chr3_1:67433785-67433804 Msa0370030:CDS 15.0%
!! TTTAATAAAATGCATTTGAT+AGG + chr3_1:67433941-67433960 None:intergenic 15.0%
!! TAATATTTCAAAATAGTCTA+TGG - chr3_1:67434930-67434949 Msa0370030:intron 15.0%
!!! TCTTCGTTTTTTTTTTTTTT+TGG - chr3_1:67435407-67435426 Msa0370030:intron 15.0%
!! TTATATATCAAACCAGAAAA+TGG - chr3_1:67432710-67432729 Msa0370030:three_prime_UTR 20.0%
!! ATATAGTTATTATTAGATGG+CGG + chr3_1:67432773-67432792 None:intergenic 20.0%
!!! ATGCTTTTACTTTGTTTTTT+AGG - chr3_1:67432958-67432977 Msa0370030:three_prime_UTR 20.0%
!! AAATAGAGAAAAAAAACTCA+AGG + chr3_1:67433093-67433112 None:intergenic 20.0%
!! AATTTCAACAACAAATTAGA+CGG - chr3_1:67433204-67433223 Msa0370030:CDS 20.0%
!! ATGTGTTAAATTATGTTTAG+TGG - chr3_1:67433241-67433260 Msa0370030:intron 20.0%
!! TGATTAATGAATATAACTAG+TGG - chr3_1:67433265-67433284 Msa0370030:intron 20.0%
!! TGCATATTTATTATGACAAT+CGG + chr3_1:67433390-67433409 None:intergenic 20.0%
!! TAAAAGGAGAAAAATAATCA+TGG - chr3_1:67433572-67433591 Msa0370030:CDS 20.0%
!!! ATGTTAGTAAATTTAGAGTA+TGG - chr3_1:67433608-67433627 Msa0370030:CDS 20.0%
!!! ATAGTTCCATTTTTAATCTT+AGG + chr3_1:67433794-67433813 None:intergenic 20.0%
!! GAAATAAATGAGTTCAAAAA+TGG - chr3_1:67434647-67434666 Msa0370030:intron 20.0%
!!! TTTTTTTTTGGACACATATT+AGG - chr3_1:67435419-67435438 Msa0370030:intron 20.0%
! ATCACTATGTTTCTTTCTAT+AGG + chr3_1:67432487-67432506 None:intergenic 25.0%
!!! TTACTTTGTTTTTTAGGTTC+TGG - chr3_1:67432964-67432983 Msa0370030:three_prime_UTR 25.0%
! TTTCTTGATGTTAAAGAGTA+TGG - chr3_1:67433122-67433141 Msa0370030:CDS 25.0%
! GGAAATATGTTAAATGAGTT+TGG - chr3_1:67433154-67433173 Msa0370030:CDS 25.0%
! TAATGAATATAACTAGTGGA+TGG - chr3_1:67433269-67433288 Msa0370030:intron 25.0%
! GATTGTCATAATAAATATGC+AGG - chr3_1:67433389-67433408 Msa0370030:intron 25.0%
! TACTTATTGCAAAAGTCATT+TGG - chr3_1:67433423-67433442 Msa0370030:intron 25.0%
!! GAATTCTAAAGGAGAATAAA+AGG - chr3_1:67433556-67433575 Msa0370030:CDS 25.0%
! AAATAATCATGGTAGCTATA+AGG - chr3_1:67433583-67433602 Msa0370030:CDS 25.0%
! TTATCATGATGACTAAATCT+TGG - chr3_1:67433631-67433650 Msa0370030:CDS 25.0%
! AAAGCAATTCAAGTCTTTAT+TGG - chr3_1:67433678-67433697 Msa0370030:CDS 25.0%
!!! ACTTCTATTTTTGCTTTCTT+AGG - chr3_1:67433892-67433911 Msa0370030:intron 25.0%
!!! TGGTTTGGTTTTGTTTTTAA+AGG - chr3_1:67434049-67434068 Msa0370030:intron 25.0%
! ATAAGTGTGATTTAAAGACT+AGG - chr3_1:67434224-67434243 Msa0370030:CDS 25.0%
! CATATTAAGAAGAGTTTGTA+AGG - chr3_1:67434399-67434418 Msa0370030:intron 25.0%
! ATAAATGAGTTCAAAAATGG+TGG - chr3_1:67434650-67434669 Msa0370030:intron 25.0%
!!! AAAACTTACTGATTTCTTGA+GGG + chr3_1:67434852-67434871 None:intergenic 25.0%
!!! AAAAACTTACTGATTTCTTG+AGG + chr3_1:67434853-67434872 None:intergenic 25.0%
!! GAATTTTTGACACATGATTT+TGG - chr3_1:67434990-67435009 Msa0370030:intron 25.0%
!! ATTGCTATAAACCTGATTTT+TGG - chr3_1:67435027-67435046 Msa0370030:intron 25.0%
! AAAAGAAAATGAAACGCAAA+TGG + chr3_1:67435206-67435225 None:intergenic 25.0%
!! ATTTGCGTTTCATTTTCTTT+TGG - chr3_1:67435205-67435224 Msa0370030:intron 25.0%
!! TTTGCGTTTCATTTTCTTTT+GGG - chr3_1:67435206-67435225 Msa0370030:intron 25.0%
! GATTATGTACCTATTTGTTT+GGG - chr3_1:67435276-67435295 Msa0370030:CDS 25.0%
! TTGTACAATAGTGAATTTGT+TGG - chr3_1:67435346-67435365 Msa0370030:intron 25.0%
!! TGTACAATAGTGAATTTGTT+GGG - chr3_1:67435347-67435366 Msa0370030:intron 25.0%
! GTTGGAAGAGATATTATATT+AGG - chr3_1:67435383-67435402 Msa0370030:intron 25.0%
!! TTTCAATACATTTTTCGCTT+AGG - chr3_1:67435677-67435696 Msa0370030:CDS 25.0%
! AAAGAAACATTGTTCTTGTT+TGG - chr3_1:67435795-67435814 Msa0370030:five_prime_UTR 25.0%
!!! TTTTCGAGTTATTTTTCGGA+AGG - chr3_1:67432397-67432416 Msa0370030:three_prime_UTR 30.0%
! CTGGTTTGATATATAAAGAC+AGG + chr3_1:67432706-67432725 None:intergenic 30.0%
! TACACAAATGTTCCATTTTC+TGG + chr3_1:67432725-67432744 None:intergenic 30.0%
! CCATTTGTTGAAACAAAGAT+GGG + chr3_1:67432818-67432837 None:intergenic 30.0%
! TCCATTTGTTGAAACAAAGA+TGG + chr3_1:67432819-67432838 None:intergenic 30.0%
! CTTTGTTTCAACAAATGGAA+AGG - chr3_1:67432820-67432839 Msa0370030:three_prime_UTR 30.0%
TGTGAAGACATAAAACAAGT+AGG - chr3_1:67432848-67432867 Msa0370030:three_prime_UTR 30.0%
! AAATTGTACTAGTTTCCTCA+CGG - chr3_1:67432928-67432947 Msa0370030:three_prime_UTR 30.0%
!! ACTTGAATTGCTTTTCAATC+AGG + chr3_1:67433672-67433691 None:intergenic 30.0%
TTGCTTTCTTAGGATCAATA+TGG - chr3_1:67433902-67433921 Msa0370030:intron 30.0%
! CTTTTACACATTTTCCAGAT+TGG - chr3_1:67434010-67434029 Msa0370030:intron 30.0%
! TCTAATCTCATTTTACAGCA+TGG + chr3_1:67434346-67434365 None:intergenic 30.0%
AAGAAGAGTTTGTAAGGAAA+TGG - chr3_1:67434405-67434424 Msa0370030:intron 30.0%
! GTAAGGAAATGGAATTTTGT+AGG - chr3_1:67434416-67434435 Msa0370030:intron 30.0%
! CTTTTATATCGAAACTAAGC+TGG - chr3_1:67434537-67434556 Msa0370030:intron 30.0%
!! TGGTGATATTAGCTTTGTTA+TGG - chr3_1:67434606-67434625 Msa0370030:intron 30.0%
!! GGTGATATTAGCTTTGTTAT+GGG - chr3_1:67434607-67434626 Msa0370030:intron 30.0%
!! GTGATATTAGCTTTGTTATG+GGG - chr3_1:67434608-67434627 Msa0370030:intron 30.0%
!!! TTTTTGAAATGGCTTCGATT+TGG - chr3_1:67434776-67434795 Msa0370030:intron 30.0%
!!! TTTTGAAATGGCTTCGATTT+GGG - chr3_1:67434777-67434796 Msa0370030:intron 30.0%
! TTTTTGACACATGATTTTGG+AGG - chr3_1:67434993-67435012 Msa0370030:intron 30.0%
AATTGTGATACTGTGATAAC+AGG - chr3_1:67435067-67435086 Msa0370030:intron 30.0%
TGATAGAAAACAAGCATCTT+TGG - chr3_1:67435155-67435174 Msa0370030:intron 30.0%
GGATTATGTACCTATTTGTT+TGG - chr3_1:67435275-67435294 Msa0370030:CDS 30.0%
CAAATATTTCGCATTTGACA+CGG + chr3_1:67435609-67435628 None:intergenic 30.0%
TGAACTGCAAGATTCTTATT+CGG - chr3_1:67435627-67435646 Msa0370030:CDS 30.0%
! CATTTTTCGCTTAGGAAAAA+AGG - chr3_1:67435685-67435704 Msa0370030:CDS 30.0%
!!! TTTCTCTAGCTTTTTGATGA+GGG - chr3_1:67435841-67435860 Msa0370030:five_prime_UTR 30.0%
TGTTGATCTTTGCTCTTTCA+AGG - chr3_1:67432516-67432535 Msa0370030:three_prime_UTR 35.0%
GTGTGTCAAAATGTAGATGT+TGG - chr3_1:67432590-67432609 Msa0370030:three_prime_UTR 35.0%
!! ATATAGCTTTTCAAGGAGGA+AGG - chr3_1:67432657-67432676 Msa0370030:three_prime_UTR 35.0%
ATTTGTGTACAATCCTGCAA+TGG - chr3_1:67432736-67432755 Msa0370030:three_prime_UTR 35.0%
! CCCATCTTTGTTTCAACAAA+TGG - chr3_1:67432815-67432834 Msa0370030:three_prime_UTR 35.0%
GAAACAAATTCCAGTAGCTT+GGG + chr3_1:67433007-67433026 None:intergenic 35.0%
TGTTTCGTGTCTAACTCATT+TGG - chr3_1:67433021-67433040 Msa0370030:exon 35.0%
!! CTAACTCATTTGGCTGTTTT+TGG - chr3_1:67433031-67433050 Msa0370030:exon 35.0%
! TAAAGAGTATGGTAGATGCT+AGG - chr3_1:67433133-67433152 Msa0370030:CDS 35.0%
CTACTCGACGCAATTAAATT+AGG - chr3_1:67433328-67433347 Msa0370030:intron 35.0%
TAAATATGCAGGTCTTAACG+AGG - chr3_1:67433400-67433419 Msa0370030:intron 35.0%
GTAAAAAACGGTATGATCAC+TGG + chr3_1:67433469-67433488 None:intergenic 35.0%
TGGCAGATCAAGGTAAAAAA+CGG + chr3_1:67433481-67433500 None:intergenic 35.0%
TAAGAATCAGTGAATGCAGA+AGG + chr3_1:67433523-67433542 None:intergenic 35.0%
GCTGCATTAACTAACATGTT+TGG - chr3_1:67433705-67433724 Msa0370030:CDS 35.0%
AAAATGGAACTATCTGTTCC+AGG - chr3_1:67433801-67433820 Msa0370030:CDS 35.0%
!! CAGATTGGTTCCTTTTTGAT+GGG - chr3_1:67434025-67434044 Msa0370030:intron 35.0%
TATATCGAAACTAAGCTGGA+AGG - chr3_1:67434541-67434560 Msa0370030:intron 35.0%
AATGAGTTCAAAAATGGTGG+TGG - chr3_1:67434653-67434672 Msa0370030:intron 35.0%
!!! ATAAACCTGATTTTTGGTGC+AGG - chr3_1:67435033-67435052 Msa0370030:intron 35.0%
GTGATAACAGGACTTCAATT+TGG - chr3_1:67435079-67435098 Msa0370030:intron 35.0%
AGGACTTCAATTTGGTCTAT+TGG - chr3_1:67435087-67435106 Msa0370030:intron 35.0%
GGACTTCAATTTGGTCTATT+GGG - chr3_1:67435088-67435107 Msa0370030:intron 35.0%
TCACTCACATTCTTGAAACA+GGG + chr3_1:67435249-67435268 None:intergenic 35.0%
TACCTATTTGTTTGGGTGTT+TGG - chr3_1:67435283-67435302 Msa0370030:CDS 35.0%
!! AAGTTTTATCATGTGTGCTC+TGG - chr3_1:67435323-67435342 Msa0370030:intron 35.0%
ACACATATTAGGATCTTCGT+GGG - chr3_1:67435430-67435449 Msa0370030:intron 35.0%
!! ATCAATTGTTTTGCATTGCG+CGG - chr3_1:67435583-67435602 Msa0370030:CDS 35.0%
GGAAAAAAGGTTATGAACGT+TGG - chr3_1:67435698-67435717 Msa0370030:CDS 35.0%
!!! CTTTCTCTAGCTTTTTGATG+AGG - chr3_1:67435840-67435859 Msa0370030:five_prime_UTR 35.0%
! GCAGGAGATATAGCTTTTCA+AGG - chr3_1:67432650-67432669 Msa0370030:three_prime_UTR 40.0%
!! GGAGATATAGCTTTTCAAGG+AGG - chr3_1:67432653-67432672 Msa0370030:three_prime_UTR 40.0%
AAGATGGGAATGTGTTCTTG+TGG + chr3_1:67432803-67432822 None:intergenic 40.0%
CATGAAATTACCCAAGCTAC+TGG - chr3_1:67432994-67433013 Msa0370030:three_prime_UTR 40.0%
CGAAACAAATTCCAGTAGCT+TGG + chr3_1:67433008-67433027 None:intergenic 40.0%
!!! TTGGCTGTTTTTGGCATTCT+TGG - chr3_1:67433040-67433059 Msa0370030:CDS 40.0%
!!! TGGCTGTTTTTGGCATTCTT+GGG - chr3_1:67433041-67433060 Msa0370030:CDS 40.0%
TGCGTCGAGTAGATGATTAA+TGG + chr3_1:67433320-67433339 None:intergenic 40.0%
CGACGCAATTAAATTAGGAG+AGG - chr3_1:67433333-67433352 Msa0370030:intron 40.0%
CAAAAGTCATTTGGACCAAG+TGG - chr3_1:67433432-67433451 Msa0370030:intron 40.0%
! TCATTTGGACCAAGTGGTTT+TGG - chr3_1:67433438-67433457 Msa0370030:CDS 40.0%
! CCATATTTGATGGCAGATCA+AGG + chr3_1:67433491-67433510 None:intergenic 40.0%
CCTTGATCTGCCATCAAATA+TGG - chr3_1:67433488-67433507 Msa0370030:CDS 40.0%
AATGCTTCAAGCTCAAAACC+TGG + chr3_1:67433822-67433841 None:intergenic 40.0%
CCATCAAAAAGGAACCAATC+TGG + chr3_1:67434027-67434046 None:intergenic 40.0%
!! CCAGATTGGTTCCTTTTTGA+TGG - chr3_1:67434024-67434043 Msa0370030:intron 40.0%
! TTGGTTCCTTTTTGATGGGA+TGG - chr3_1:67434029-67434048 Msa0370030:intron 40.0%
! TCCTTTTTGATGGGATGGTT+TGG - chr3_1:67434034-67434053 Msa0370030:intron 40.0%
ACCAAACCATCCCATCAAAA+AGG + chr3_1:67434038-67434057 None:intergenic 40.0%
GGCTAGATGTTCAGATACAT+AGG + chr3_1:67434083-67434102 None:intergenic 40.0%
GTATCTGAACATCTAGCCAT+TGG - chr3_1:67434085-67434104 Msa0370030:CDS 40.0%
GTAGCATTGTCGATCATCAT+TGG - chr3_1:67434449-67434468 Msa0370030:intron 40.0%
!! TGTCGATCATCATTGGTTTC+TGG - chr3_1:67434456-67434475 Msa0370030:intron 40.0%
!! GATCATCATTGGTTTCTGGA+CGG - chr3_1:67434460-67434479 Msa0370030:intron 40.0%
TGATCACTACCAATTGACGA+AGG + chr3_1:67434577-67434596 None:intergenic 40.0%
GGTAGTGATCATAGTGTTGT+TGG - chr3_1:67434586-67434605 Msa0370030:intron 40.0%
AACCTAATCCATACACCAAG+AGG + chr3_1:67434713-67434732 None:intergenic 40.0%
AGACTTAACGAGTGCGAAAT+TGG - chr3_1:67434746-67434765 Msa0370030:intron 40.0%
!! TTGGAGGAGGGTTTTTGAAA+TGG - chr3_1:67434765-67434784 Msa0370030:intron 40.0%
GATACAAGCAGCAGAAACAT+TGG + chr3_1:67434813-67434832 None:intergenic 40.0%
! TGTTTCTGCTGCTTGTATCA+CGG - chr3_1:67434814-67434833 Msa0370030:CDS 40.0%
!! ACTTACTGATTTCTTGAGGG+TGG + chr3_1:67434849-67434868 None:intergenic 40.0%
ATTCACCTGCACCAAAAATC+AGG + chr3_1:67435041-67435060 None:intergenic 40.0%
!! GAGAGCATTTGTCTATGCTA+TGG - chr3_1:67435177-67435196 Msa0370030:intron 40.0%
CTCACTCACATTCTTGAAAC+AGG + chr3_1:67435250-67435269 None:intergenic 40.0%
TCAAGAATGTGAGTGAGCAT+AGG - chr3_1:67435253-67435272 Msa0370030:CDS 40.0%
CAAGAATGTGAGTGAGCATA+GGG - chr3_1:67435254-67435273 Msa0370030:CDS 40.0%
AGCCAAACACCCAAACAAAT+AGG + chr3_1:67435288-67435307 None:intergenic 40.0%
!! GAATTTGTTGGGTCTGAGTT+AGG - chr3_1:67435358-67435377 Msa0370030:intron 40.0%
GACACATATTAGGATCTTCG+TGG - chr3_1:67435429-67435448 Msa0370030:intron 40.0%
ATTAGGATCTTCGTGGGATT+TGG - chr3_1:67435436-67435455 Msa0370030:CDS 40.0%
ACTGCAAGATTCTTATTCGG+TGG - chr3_1:67435630-67435649 Msa0370030:CDS 40.0%
CTGCAAGATTCTTATTCGGT+GGG - chr3_1:67435631-67435650 Msa0370030:CDS 40.0%
!!! TCTAGCTTTTTGATGAGGGT+GGG - chr3_1:67435845-67435864 Msa0370030:five_prime_UTR 40.0%
GGCAATTCATGTTCCATTGC+AGG + chr3_1:67432752-67432771 None:intergenic 45.0%
! TACTAGTTTCCTCACGGTTG+AGG - chr3_1:67432934-67432953 Msa0370030:three_prime_UTR 45.0%
!!! GGCTGTTTTTGGCATTCTTG+GGG - chr3_1:67433042-67433061 Msa0370030:CDS 45.0%
GAGAGGCGAACCATATTTGA+TGG + chr3_1:67433501-67433520 None:intergenic 45.0%
ATCAGTGAATGCAGAAGGAG+AGG + chr3_1:67433518-67433537 None:intergenic 45.0%
! CTAGCCATTGGTCTAACAAC+CGG - chr3_1:67434097-67434116 Msa0370030:CDS 45.0%
GTCTAACAACCGGCTACTTA+GGG - chr3_1:67434107-67434126 Msa0370030:CDS 45.0%
CACTGGATCTTGTCTTTGCT+CGG - chr3_1:67434187-67434206 Msa0370030:CDS 45.0%
! TCTTTGCTCGGCTTTGTACT+AGG - chr3_1:67434199-67434218 Msa0370030:CDS 45.0%
TGACAGCTTCCTTCGTCAAT+TGG - chr3_1:67434565-67434584 Msa0370030:intron 45.0%
!! TTGTGGTTAGCCTGCATTGT+TGG - chr3_1:67434686-67434705 Msa0370030:intron 45.0%
! TTGTTGGACCTCTTGGTGTA+TGG - chr3_1:67434702-67434721 Msa0370030:intron 45.0%
! GACCTCTTGGTGTATGGATT+AGG - chr3_1:67434708-67434727 Msa0370030:intron 45.0%
CTTAACGAGTGCGAAATTGG+AGG - chr3_1:67434749-67434768 Msa0370030:intron 45.0%
TTGGGTCTGAGTTAGGAAGT+TGG - chr3_1:67435365-67435384 Msa0370030:intron 45.0%
!!! CTCTAGCTTTTTGATGAGGG+TGG - chr3_1:67435844-67435863 Msa0370030:five_prime_UTR 45.0%
TGCTCTTTCAAGGATCGCCA+TGG - chr3_1:67432526-67432545 Msa0370030:three_prime_UTR 50.0%
GTTGGGGTTCTGCAGTTAGT+AGG - chr3_1:67432561-67432580 Msa0370030:three_prime_UTR 50.0%
GAAGCTGAGAATCGTGAGTG+TGG - chr3_1:67432620-67432639 Msa0370030:three_prime_UTR 50.0%
GGAGGAAGGAAAAGTGAGAG+TGG - chr3_1:67432671-67432690 Msa0370030:three_prime_UTR 50.0%
GGAAGGAAAAGTGAGAGTGG+TGG - chr3_1:67432674-67432693 Msa0370030:three_prime_UTR 50.0%
! AAAAGCATGCCTCAACCGTG+AGG + chr3_1:67432946-67432965 None:intergenic 50.0%
GTAGCCGGTTGTTAGACCAA+TGG + chr3_1:67434104-67434123 None:intergenic 50.0%
GGTCTAACAACCGGCTACTT+AGG - chr3_1:67434106-67434125 Msa0370030:CDS 50.0%
TGTTAGGCTCCCTAAGTAGC+CGG + chr3_1:67434119-67434138 None:intergenic 50.0%
GGGAGCCTAACAACTTTCAG+CGG - chr3_1:67434127-67434146 Msa0370030:CDS 50.0%
TCCAGCCGCTGAAAGTTGTT+AGG + chr3_1:67434135-67434154 None:intergenic 50.0%
! ATTGGTTTCTGGACGGCGAA+GGG - chr3_1:67434467-67434486 Msa0370030:intron 50.0%
ATGGGGTGTGAGTAGTGCTT+TGG - chr3_1:67434625-67434644 Msa0370030:intron 50.0%
ACCAAGAGGTCCAACAATGC+AGG + chr3_1:67434699-67434718 None:intergenic 50.0%
AACGAGTGCGAAATTGGAGG+AGG - chr3_1:67434752-67434771 Msa0370030:intron 50.0%
ACGAGTGCGAAATTGGAGGA+GGG - chr3_1:67434753-67434772 Msa0370030:intron 50.0%
CATGGAGCGAACGAGCAGTT+GGG - chr3_1:67432544-67432563 Msa0370030:three_prime_UTR 55.0%
ATGGAGCGAACGAGCAGTTG+GGG - chr3_1:67432545-67432564 Msa0370030:three_prime_UTR 55.0%
CTGGTCACGCCAAAACCACT+TGG + chr3_1:67433450-67433469 None:intergenic 55.0%
GCCTAACAACTTTCAGCGGC+TGG - chr3_1:67434131-67434150 Msa0370030:CDS 55.0%
!! CATTGGTTTCTGGACGGCGA+AGG - chr3_1:67434466-67434485 Msa0370030:intron 55.0%
GGACGGCGAAGGGATTCAAA+TGG - chr3_1:67434477-67434496 Msa0370030:intron 55.0%
! GTGGTGGTGAAGCTCAGTTG+TGG - chr3_1:67434669-67434688 Msa0370030:intron 55.0%
GCCTGCATTGTTGGACCTCT+TGG - chr3_1:67434695-67434714 Msa0370030:intron 55.0%
CCAACTGCTCGTTCGCTCCA+TGG + chr3_1:67432546-67432565 None:intergenic 60.0%
CCATGGAGCGAACGAGCAGT+TGG - chr3_1:67432543-67432562 Msa0370030:three_prime_UTR 60.0%
CGTGAGTGTGGTTCAGCTGC+AGG - chr3_1:67432632-67432651 Msa0370030:three_prime_UTR 60.0%
ATGCTCGAGCTCAGTGTCGC+TGG - chr3_1:67434163-67434182 Msa0370030:CDS 60.0%
! AGCTCAGTGTCGCTGGACAC+TGG - chr3_1:67434170-67434189 Msa0370030:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 67432393 67435905 67432393 ID=Msa0370030;Name=Msa0370030
chr3_1 mRNA 67432393 67435905 67432393 ID=Msa0370030-mRNA-1;Parent=Msa0370030;Name=Msa0370030-mRNA-1;_AED=0.31;_eAED=0.31;_QI=152|1|1|1|1|1|6|641|443
chr3_1 exon 67432393 67433243 67432393 ID=Msa0370030-mRNA-1:exon:13937;Parent=Msa0370030-mRNA-1
chr3_1 exon 67433436 67433860 67433436 ID=Msa0370030-mRNA-1:exon:13936;Parent=Msa0370030-mRNA-1
chr3_1 exon 67434078 67434270 67434078 ID=Msa0370030-mRNA-1:exon:13935;Parent=Msa0370030-mRNA-1
chr3_1 exon 67434789 67434887 67434789 ID=Msa0370030-mRNA-1:exon:13934;Parent=Msa0370030-mRNA-1
chr3_1 exon 67435235 67435318 67435235 ID=Msa0370030-mRNA-1:exon:13933;Parent=Msa0370030-mRNA-1
chr3_1 exon 67435433 67435905 67435433 ID=Msa0370030-mRNA-1:exon:13932;Parent=Msa0370030-mRNA-1
chr3_1 five_prime_UTR 67435754 67435905 67435754 ID=Msa0370030-mRNA-1:five_prime_utr;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67435433 67435753 67435433 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67435235 67435318 67435235 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67434789 67434887 67434789 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67434078 67434270 67434078 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67433436 67433860 67433436 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 CDS 67433034 67433243 67433034 ID=Msa0370030-mRNA-1:cds;Parent=Msa0370030-mRNA-1
chr3_1 three_prime_UTR 67432393 67433033 67432393 ID=Msa0370030-mRNA-1:three_prime_utr;Parent=Msa0370030-mRNA-1
Gene Sequence

>Msa0370030

ATGGAGCGAACGAGCAGTTGGGGTTCTGCAGTTAGTAGGCTTTCAGTGTGTCAAAATGTAGATGTTGGTGACGAAGAAGCTGAGAATCGTGAGTGTGGTTCAGCTGCAGGAGATATAGCTTTTCAAGGAGGAAGGAAAAGTGAGAGTGGTGGCTTTGACCTGTCTTTATATATCAAACCAGAAAATGGAACATTTGTGTACAATCCTGCAATGGAACATGAATTGCCGCCATCTAATAATAACTATATTGTAAATCCACAAGAACACATTCCCATCTTTGTTTCAACAAATGGAAAGGTTAATTGTGAAGACATAAAACAAGTTCTGGAACTTGACATGAAATTACCCAAGCTACTGGAATTTGTTTCGTGTCTAACTCATTTGGCTGTTTTTGGCATTCTTGGGGTCTTAACGAGGTACTTATTGCAAAAGTCATTTGGACCAAGTGGTTTTGGCGTGACCAGTGATCATACCGTTTTTTACCTTGATCTGCCATCAAATATGATTGGTTCCTTTTTGATGGGATGGTTTGGTTTTGTTTTTAAAGGAGATATATCCTATGTATCTGAACATCTAGCCATTGGTCTAACAACCGGCTACTTAGGGAGCCTAACAACTTTCAGCGGCTGGAATCAGAAAATGCTCGAGCTCAGTGTCGCTGGACACTGGATCTTGTCTTTGCTCGGCTTTGTACTAGGCATGTTTCTTGTAGCATTGTCGATCATCATTGGTTTCTGGACGGCGAAGGGATTCAAATGGTTACTCAAAAGACTAAACATGAGACATGAAAGTGACTCTTTTATATCGAAACTAAGCTGGAAGGTTGACAGCTTCCTTCGTCAATTGGTAGTGATCATAGTGTTGTTGGTGATATTAGCTTTGTTATGGGGTGTGAGTAGTGCTTTGGAAATAAATGAGTTCAAAAATGGTGGTGGTGAAGCTCAGTTGTGGTTAGCCTGCATTGTTGGACCTCTTGGTGTATGGATTAGGTTTTTCTTAGCTACTAGACTTAACGAGTGCGAAATTGGAGGAGGGTTTTTGAAATGGCTTCGATTTGGGACTCTTATTGCCAATGTTTCTGCTGCTTGTATCACGGCTGCACTTGCCACCCTCAAGAAATCAGTGAATACTACAAATTGTGATACTGTGATAACAGGACTTCAATTTGGTCTATTGGGATGTCTGAGTACTGTTTCAACATTTGTTGCTGAATTGTATGAAATGATAGAAAACAAGCATCTTTGGAGAGCATTTGTCTATGCTATGGTTACCATTTGCGTTTCATTTTCTTTTGGGATCTTATTATACTATATCCCTGTTTCAAGAATGTGA
Protein sequence

>Msa0370030

MERTSSWGSAVSRLSVCQNVDVGDEEAENRECGSAAGDIAFQGGRKSESGGFDLSLYIKPENGTFVYNPAMEHELPPSNNNYIVNPQEHIPIFVSTNGKVNCEDIKQVLELDMKLPKLLEFVSCLTHLAVFGILGVLTRYLLQKSFGPSGFGVTSDHTVFYLDLPSNMIGSFLMGWFGFVFKGDISYVSEHLAIGLTTGYLGSLTTFSGWNQKMLELSVAGHWILSLLGFVLGMFLVALSIIIGFWTAKGFKWLLKRLNMRHESDSFISKLSWKVDSFLRQLVVIIVLLVILALLWGVSSALEINEFKNGGGEAQLWLACIVGPLGVWIRFFLATRLNECEIGGGFLKWLRFGTLIANVSAACITAALATLKKSVNTTNCDTVITGLQFGLLGCLSTVSTFVAELYEMIENKHLWRAFVYAMVTICVSFSFGILLYYIPVSRM*