AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1406780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1406780 MtrunA17_Chr7g0274631 97.647 170 3 1 1 169 1 170 3.14e-124 346
Msa1406780 MtrunA17_Chr5g0431211 72.263 137 31 4 26 158 36 169 8.43e-68 205
Msa1406780 MtrunA17_Chr4g0051251 68.571 140 37 4 25 160 44 180 2.27e-64 197
Msa1406780 MtrunA17_Chr1g0167411 73.438 128 28 3 27 151 65 189 3.21e-64 196
Msa1406780 MtrunA17_Chr2g0283861 71.318 129 31 3 25 150 33 158 7.14e-63 191
Msa1406780 MtrunA17_Chr1g0190231 73.288 146 33 3 27 169 40 182 1.66e-62 191
Msa1406780 MtrunA17_Chr2g0325831 65.306 147 45 3 16 159 12 155 3.45e-62 189
Msa1406780 MtrunA17_Chr2g0315791 68.217 129 35 3 25 150 31 156 4.06e-60 184
Msa1406780 MtrunA17_Chr1g0187671 70.213 141 36 3 19 156 45 182 7.23e-60 184
Msa1406780 MtrunA17_Chr4g0041141 66.667 129 37 3 25 150 27 152 1.41e-59 183
Msa1406780 MtrunA17_Chr7g0261381 72.263 137 32 3 23 156 60 193 3.61e-59 183
Msa1406780 MtrunA17_Chr1g0183761 73.684 133 29 3 27 156 45 174 1.18e-58 182
Msa1406780 MtrunA17_Chr3g0105921 68.276 145 39 4 27 167 42 183 1.15e-56 177
Msa1406780 MtrunA17_Chr8g0379061 68.462 130 33 4 25 150 83 208 5.48e-47 153
Msa1406780 MtrunA17_Chr1g0211641 71.739 46 12 1 20 65 64 108 6.14e-18 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1406780 AT5G58500.1 70.423 142 36 4 26 163 18 157 1.71e-67 203
Msa1406780 AT1G07090.1 65.493 142 43 3 26 164 30 168 1.55e-64 196
Msa1406780 AT5G28490.1 76.471 136 26 3 27 159 25 157 1.67e-62 191
Msa1406780 AT3G04510.1 70.714 140 35 3 27 163 33 169 8.90e-60 184
Msa1406780 AT2G42610.1 67.969 128 35 3 26 150 24 148 2.65e-59 182
Msa1406780 AT2G42610.2 67.969 128 35 3 26 150 24 148 2.65e-59 182
Msa1406780 AT2G31160.1 75.556 135 26 4 27 157 54 185 4.71e-58 181
Msa1406780 AT4G18610.1 65.116 129 38 3 26 150 36 161 2.26e-57 178
Msa1406780 AT3G23290.2 75.573 131 26 3 23 150 44 171 2.79e-57 178
Msa1406780 AT1G78815.1 63.566 129 41 3 25 150 38 163 3.08e-55 173
Msa1406780 AT1G16910.1 58.955 134 49 3 25 155 21 151 3.35e-52 164

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACTACCTTGATCAATT+TGG 0.137888 tig0023244:+20147 Msa1406780:CDS
AATCCAACTTGTCCTTTCTT+TGG 0.215866 tig0023244:+20186 Msa1406780:CDS
TGTTCCAGTCTCTCCTCTTT+TGG 0.217847 tig0023244:-20044 None:intergenic
ATCCAACTTGTCCTTTCTTT+GGG 0.324334 tig0023244:+20187 Msa1406780:CDS
TTCACTACCTTGATCAATTT+GGG 0.326576 tig0023244:+20148 Msa1406780:CDS
TTGTCCTCTCCGTCAGGCTT+GGG 0.343185 tig0023244:+20236 Msa1406780:CDS
CTTGTCCTCTCCGTCAGGCT+TGG 0.356689 tig0023244:+20235 Msa1406780:CDS
CATGGTGGAAGTAACCCTTT+TGG 0.395005 tig0023244:+20312 Msa1406780:CDS
GCACATGGAGATGGAGGGTT+TGG 0.411549 tig0023244:-20213 None:intergenic
CACTTCCATTGTAGCTGCTA+GGG 0.441671 tig0023244:-19984 None:intergenic
GTCGATGGTTTCTTAAGTAC+TGG 0.444811 tig0023244:-20074 None:intergenic
ATGTGCTTGTCCTCTCCGTC+AGG 0.459665 tig0023244:+20230 Msa1406780:CDS
TGATCAATTTGGGAAGACTA+AGG 0.465045 tig0023244:+20158 Msa1406780:CDS
GTGGTGGCAACAAGATCAAC+AGG 0.470799 tig0023244:+20013 Msa1406780:CDS
CTAACAGCGCCATCACCAAA+AGG 0.486109 tig0023244:-20327 None:intergenic
GAGTTAGCTACCATAAGAAA+AGG 0.502671 tig0023244:+20394 Msa1406780:CDS
TAGTCTTCCCAAATTGATCA+AGG 0.503689 tig0023244:-20155 None:intergenic
GTAGTTGTGATGTTGCGGTT+AGG 0.503903 tig0023244:-20423 None:intergenic
AGAGCCAAAAGAGGAGAGAC+TGG 0.512337 tig0023244:+20040 Msa1406780:CDS
GCTTATGATCAAGAGATTCA+TGG 0.517251 tig0023244:+20294 Msa1406780:CDS
ATTGAAGCAGAAAGTGGTAC+TGG 0.535622 tig0023244:-20096 None:intergenic
TCACTTCCATTGTAGCTGCT+AGG 0.535763 tig0023244:-19985 None:intergenic
TTATACAAGAAATATGGCAT+TGG 0.537262 tig0023244:+19942 None:intergenic
CTTTCTGCTTCAATGTGCAA+TGG 0.545802 tig0023244:+20105 Msa1406780:CDS
TGTCCTCTCCGTCAGGCTTG+GGG 0.552431 tig0023244:+20237 Msa1406780:CDS
GGTAGCCTTGATGCTCTCAT+CGG 0.556787 tig0023244:+20258 Msa1406780:CDS
AGAGGACAAGCACATGGAGA+TGG 0.557220 tig0023244:-20222 None:intergenic
CAAATTGATCAAGGTAGTGA+AGG 0.557585 tig0023244:-20146 None:intergenic
GGGTTTGGCATCCCAAAGAA+AGG 0.560750 tig0023244:-20198 None:intergenic
ACAAACCCTAGCAGCTACAA+TGG 0.562580 tig0023244:+19979 Msa1406780:CDS
TTGCACATTGAAGCAGAAAG+TGG 0.564133 tig0023244:-20102 None:intergenic
TAACAGCGCCATCACCAAAA+GGG 0.569402 tig0023244:-20326 None:intergenic
CATGACAAATAGAAATCAAG+AGG 0.576026 tig0023244:-20457 None:intergenic
GGACAAGCACATGGAGATGG+AGG 0.578694 tig0023244:-20219 None:intergenic
ATCCCAAAGAAAGGACAAGT+TGG 0.587746 tig0023244:-20189 None:intergenic
CAGAAAGTGGTACTGGTCGA+TGG 0.588396 tig0023244:-20089 None:intergenic
GAGAAGTAGTTGTGATGTTG+CGG 0.588969 tig0023244:-20428 None:intergenic
CAAGGCTACCCCAAGCCTGA+CGG 0.597531 tig0023244:-20245 None:intergenic
ACAGGTACGAGAGCCAAAAG+AGG 0.613687 tig0023244:+20031 Msa1406780:CDS
TGACGGAGAGGACAAGCACA+TGG 0.624357 tig0023244:-20228 None:intergenic
AATGGAAGTGATTCAAGTGG+TGG 0.625867 tig0023244:+19997 Msa1406780:CDS
GGCGACCGATGAGAGCATCA+AGG 0.637841 tig0023244:-20263 None:intergenic
GACAAGCACATGGAGATGGA+GGG 0.652537 tig0023244:-20218 None:intergenic
CTACCCCAAGCCTGACGGAG+AGG 0.655871 tig0023244:-20240 None:intergenic
TACAATGGAAGTGATTCAAG+TGG 0.673612 tig0023244:+19994 Msa1406780:CDS
CTTGATCATAAGCAGCGCGG+AGG 0.674942 tig0023244:-20284 None:intergenic
TATGATCAAGAGATTCATGG+TGG 0.715975 tig0023244:+20297 Msa1406780:CDS
TCTCTTGATCATAAGCAGCG+CGG 0.775865 tig0023244:-20287 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTCACTACCTTGATCAATTT+GGG + tig0023244:20148-20167 Msa1406780:CDS 30.0%
TACAATGGAAGTGATTCAAG+TGG + tig0023244:19994-20013 Msa1406780:CDS 35.0%
CAAATTGATCAAGGTAGTGA+AGG - tig0023244:20149-20168 None:intergenic 35.0%
CTTCACTACCTTGATCAATT+TGG + tig0023244:20147-20166 Msa1406780:CDS 35.0%
TAGTCTTCCCAAATTGATCA+AGG - tig0023244:20158-20177 None:intergenic 35.0%
TGATCAATTTGGGAAGACTA+AGG + tig0023244:20158-20177 Msa1406780:CDS 35.0%
AATCCAACTTGTCCTTTCTT+TGG + tig0023244:20186-20205 Msa1406780:CDS 35.0%
ATCCAACTTGTCCTTTCTTT+GGG + tig0023244:20187-20206 Msa1406780:CDS 35.0%
GCTTATGATCAAGAGATTCA+TGG + tig0023244:20294-20313 Msa1406780:CDS 35.0%
TATGATCAAGAGATTCATGG+TGG + tig0023244:20297-20316 Msa1406780:CDS 35.0%
! TGTTCGTGATTTTCAGTCAA+AGG + tig0023244:20365-20384 Msa1406780:CDS 35.0%
GAGTTAGCTACCATAAGAAA+AGG + tig0023244:20394-20413 Msa1406780:CDS 35.0%
! TAGGTCTCTTCCTTTTCTTA+TGG - tig0023244:20407-20426 None:intergenic 35.0%
AATGGAAGTGATTCAAGTGG+TGG + tig0023244:19997-20016 Msa1406780:CDS 40.0%
GTCGATGGTTTCTTAAGTAC+TGG - tig0023244:20077-20096 None:intergenic 40.0%
ATTGAAGCAGAAAGTGGTAC+TGG - tig0023244:20099-20118 None:intergenic 40.0%
TTGCACATTGAAGCAGAAAG+TGG - tig0023244:20105-20124 None:intergenic 40.0%
CTTTCTGCTTCAATGTGCAA+TGG + tig0023244:20105-20124 Msa1406780:CDS 40.0%
ATCCCAAAGAAAGGACAAGT+TGG - tig0023244:20192-20211 None:intergenic 40.0%
! GATTTTCAGTCAAAGGCTAG+AGG + tig0023244:20372-20391 Msa1406780:CDS 40.0%
GAGAAGTAGTTGTGATGTTG+CGG - tig0023244:20431-20450 None:intergenic 40.0%
ACAAACCCTAGCAGCTACAA+TGG + tig0023244:19979-19998 Msa1406780:CDS 45.0%
CACTTCCATTGTAGCTGCTA+GGG - tig0023244:19987-20006 None:intergenic 45.0%
TCACTTCCATTGTAGCTGCT+AGG - tig0023244:19988-20007 None:intergenic 45.0%
! TGTTCCAGTCTCTCCTCTTT+TGG - tig0023244:20047-20066 None:intergenic 45.0%
TCTCTTGATCATAAGCAGCG+CGG - tig0023244:20290-20309 None:intergenic 45.0%
!! CATGGTGGAAGTAACCCTTT+TGG + tig0023244:20312-20331 Msa1406780:CDS 45.0%
! GGAAGTAACCCTTTTGGTGA+TGG + tig0023244:20318-20337 Msa1406780:CDS 45.0%
TAACAGCGCCATCACCAAAA+GGG - tig0023244:20329-20348 None:intergenic 45.0%
GTAGTTGTGATGTTGCGGTT+AGG - tig0023244:20426-20445 None:intergenic 45.0%
GTGGTGGCAACAAGATCAAC+AGG + tig0023244:20013-20032 Msa1406780:CDS 50.0%
ACAGGTACGAGAGCCAAAAG+AGG + tig0023244:20031-20050 Msa1406780:CDS 50.0%
AGAGCCAAAAGAGGAGAGAC+TGG + tig0023244:20040-20059 Msa1406780:CDS 50.0%
!! CAGAAAGTGGTACTGGTCGA+TGG - tig0023244:20092-20111 None:intergenic 50.0%
GGGTTTGGCATCCCAAAGAA+AGG - tig0023244:20201-20220 None:intergenic 50.0%
GACAAGCACATGGAGATGGA+GGG - tig0023244:20221-20240 None:intergenic 50.0%
AGAGGACAAGCACATGGAGA+TGG - tig0023244:20225-20244 None:intergenic 50.0%
! GGTAGCCTTGATGCTCTCAT+CGG + tig0023244:20258-20277 Msa1406780:CDS 50.0%
CTAACAGCGCCATCACCAAA+AGG - tig0023244:20330-20349 None:intergenic 50.0%
!!! CTTTTGGTGATGGCGCTGTT+AGG + tig0023244:20328-20347 Msa1406780:CDS 50.0%
GCACATGGAGATGGAGGGTT+TGG - tig0023244:20216-20235 None:intergenic 55.0%
GGACAAGCACATGGAGATGG+AGG - tig0023244:20222-20241 None:intergenic 55.0%
TGACGGAGAGGACAAGCACA+TGG - tig0023244:20231-20250 None:intergenic 55.0%
ATGTGCTTGTCCTCTCCGTC+AGG + tig0023244:20230-20249 Msa1406780:CDS 55.0%
TTGTCCTCTCCGTCAGGCTT+GGG + tig0023244:20236-20255 Msa1406780:CDS 55.0%
CTTGATCATAAGCAGCGCGG+AGG - tig0023244:20287-20306 None:intergenic 55.0%
CTTGTCCTCTCCGTCAGGCT+TGG + tig0023244:20235-20254 Msa1406780:CDS 60.0%
TGTCCTCTCCGTCAGGCTTG+GGG + tig0023244:20237-20256 Msa1406780:CDS 60.0%
CAAGGCTACCCCAAGCCTGA+CGG - tig0023244:20248-20267 None:intergenic 60.0%
!! GGCGACCGATGAGAGCATCA+AGG - tig0023244:20266-20285 None:intergenic 60.0%
CTACCCCAAGCCTGACGGAG+AGG - tig0023244:20243-20262 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0023244 gene 19955 20464 19955 ID=Msa1406780;Name=Msa1406780
tig0023244 mRNA 19955 20464 19955 ID=Msa1406780-mRNA-1;Parent=Msa1406780;Name=Msa1406780-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|169
tig0023244 exon 19955 20464 19955 ID=Msa1406780-mRNA-1:exon:24751;Parent=Msa1406780-mRNA-1
tig0023244 CDS 19955 20464 19955 ID=Msa1406780-mRNA-1:cds;Parent=Msa1406780-mRNA-1
Gene Sequence

>Msa1406780

ATGGCATTGGTTTCAGCTGAATCAACAAACCCTAGCAGCTACAATGGAAGTGATTCAAGTGGTGGCAACAAGATCAACAGGTACGAGAGCCAAAAGAGGAGAGACTGGAACACATTCTGCCAGTACTTAAGAAACCATCGACCAGTACCACTTTCTGCTTCAATGTGCAATGGAACTCATGTTCTTGAATTCCTTCACTACCTTGATCAATTTGGGAAGACTAAGGTTCACAATCCAACTTGTCCTTTCTTTGGGATGCCAAACCCTCCATCTCCATGTGCTTGTCCTCTCCGTCAGGCTTGGGGTAGCCTTGATGCTCTCATCGGTCGCCTCCGCGCTGCTTATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGATGGCGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACCGCAACATCACAACTACTTCTCAATCATCCTCTTGA

Protein sequence

>Msa1406780

MALVSAESTNPSSYNGSDSSGGNKINRYESQKRRDWNTFCQYLRNHRPVPLSASMCNGTHVLEFLHYLDQFGKTKVHNPTCPFFGMPNPPSPCACPLRQAWGSLDALIGRLRAAYDQEIHGGSNPFGDGAVRFYLRDVRDFQSKARGVSYHKKRKRPNRNITTTSQSSS*