Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0426600 | XP_013461494.1 | 93.519 | 324 | 7 | 3 | 1 | 324 | 1 | 310 | 0.0 | 605 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0426600 | sp|Q9LY00|WRK70_ARATH | 44.144 | 222 | 106 | 5 | 13 | 234 | 7 | 210 | 1.45e-49 | 170 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0426600 | A0A072V0I2 | 93.519 | 324 | 7 | 3 | 1 | 324 | 1 | 310 | 0.0 | 605 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0426600 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0054310 | Msa0426600 | 0.812312 | 4.516777e-51 | -8.615850e-47 |
| Msa0092910 | Msa0426600 | 0.821163 | 4.671450e-53 | -8.615850e-47 |
| Msa0131420 | Msa0426600 | 0.817867 | 2.638505e-52 | -8.615850e-47 |
| Msa0148720 | Msa0426600 | 0.810046 | 1.400204e-50 | -8.615850e-47 |
| Msa0153450 | Msa0426600 | 0.820335 | 7.242545e-53 | -8.615850e-47 |
| Msa0198640 | Msa0426600 | 0.802440 | 5.603915e-49 | -8.615850e-47 |
| Msa0377800 | Msa0426600 | 0.971274 | 6.602308e-133 | -8.615850e-47 |
| Msa0414650 | Msa0426600 | 0.843577 | 1.286588e-58 | -8.615850e-47 |
| Msa0426600 | Msa0431440 | 0.829046 | 6.406568e-55 | -8.615850e-47 |
| Msa0426600 | Msa0459560 | 0.847603 | 1.041603e-59 | -8.615850e-47 |
| Msa0426600 | Msa0472910 | 0.961963 | 2.576421e-120 | -8.615850e-47 |
| Msa0426600 | Msa0476330 | 0.835789 | 1.367274e-56 | -8.615850e-47 |
| Msa0426600 | Msa0506300 | 0.845706 | 3.434586e-59 | -8.615850e-47 |
| Msa0426600 | Msa0517570 | 0.973497 | 1.574903e-136 | -8.615850e-47 |
| Msa0426600 | Msa0518280 | 0.804483 | 2.113535e-49 | -8.615850e-47 |
| Msa0426600 | Msa0520330 | 0.845469 | 3.982343e-59 | -8.615850e-47 |
| Msa0426600 | Msa0568440 | 0.812057 | 5.133283e-51 | -8.615850e-47 |
| Msa0426600 | Msa0574570 | 0.829498 | 4.978919e-55 | -8.615850e-47 |
| Msa0426600 | Msa0574580 | 0.836035 | 1.184517e-56 | -8.615850e-47 |
| Msa0426600 | Msa0650520 | 0.800896 | 1.162344e-48 | -8.615850e-47 |
| Msa0426600 | Msa0708290 | 0.825692 | 4.081031e-54 | -8.615850e-47 |
| Msa0426600 | Msa0708300 | 0.837621 | 4.664282e-57 | -8.615850e-47 |
| Msa0426600 | Msa0745070 | 0.820026 | 8.526652e-53 | -8.615850e-47 |
| Msa0426600 | Msa0777770 | 0.803183 | 3.935943e-49 | -8.615850e-47 |
| Msa0426600 | Msa0780350 | 0.817200 | 3.731325e-52 | -8.615850e-47 |
| Msa0426600 | Msa0834030 | 0.804577 | 2.020222e-49 | -8.615850e-47 |
| Msa0426600 | Msa0936530 | 0.837850 | 4.074014e-57 | -8.615850e-47 |
| Msa0426600 | Msa0936550 | 0.834505 | 2.882759e-56 | -8.615850e-47 |
| Msa0426600 | Msa0973520 | 0.816133 | 6.470422e-52 | -8.615850e-47 |
| Msa0426600 | Msa1026510 | 0.819793 | 9.637129e-53 | -8.615850e-47 |
| Msa0426600 | Msa1074580 | 0.822166 | 2.738928e-53 | -8.615850e-47 |
| Msa0426600 | Msa1154080 | 0.825239 | 5.222908e-54 | -8.615850e-47 |
| Msa0426600 | Msa1201200 | 0.827170 | 1.814206e-54 | -8.615850e-47 |
| Msa0426600 | Msa1236730 | 0.820013 | 8.582511e-53 | -8.615850e-47 |
| Msa0426600 | Msa1252960 | 0.808929 | 2.432655e-50 | -8.615850e-47 |
| Msa0426600 | Msa1269660 | 0.818405 | 1.994466e-52 | -8.615850e-47 |
| Msa0426600 | Msa1317130 | 0.838156 | 3.399024e-57 | -8.615850e-47 |
| Msa0426600 | Msa1353880 | 0.825735 | 3.985449e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0426600 | MtrunA17_Chr3g0128671 | 93.519 | 324 | 7 | 3 | 1 | 324 | 1 | 310 | 0.0 | 605 |
| Msa0426600 | MtrunA17_Chr5g0428351 | 40.741 | 297 | 97 | 12 | 5 | 289 | 82 | 311 | 1.07e-51 | 173 |
| Msa0426600 | MtrunA17_Chr7g0244971 | 39.000 | 200 | 93 | 5 | 17 | 210 | 15 | 191 | 4.34e-38 | 137 |
| Msa0426600 | MtrunA17_Chr7g0244981 | 37.915 | 211 | 100 | 7 | 7 | 210 | 4 | 190 | 1.74e-35 | 130 |
| Msa0426600 | MtrunA17_Chr8g0350621 | 38.806 | 201 | 99 | 6 | 18 | 216 | 8 | 186 | 9.88e-35 | 127 |
| Msa0426600 | MtrunA17_Chr8g0334541 | 38.571 | 210 | 97 | 6 | 18 | 226 | 6 | 184 | 2.84e-31 | 118 |
| Msa0426600 | MtrunA17_Chr3g0126391 | 33.190 | 232 | 123 | 9 | 15 | 229 | 5 | 221 | 9.27e-25 | 102 |
| Msa0426600 | MtrunA17_Chr2g0315361 | 32.819 | 259 | 132 | 10 | 14 | 256 | 13 | 245 | 1.04e-24 | 101 |
| Msa0426600 | MtrunA17_Chr2g0315371 | 33.772 | 228 | 125 | 7 | 3 | 222 | 5 | 214 | 1.88e-24 | 100 |
| Msa0426600 | MtrunA17_Chr2g0315381 | 34.783 | 230 | 115 | 8 | 9 | 222 | 7 | 217 | 3.46e-23 | 97.4 |
| Msa0426600 | MtrunA17_Chr7g0275531 | 30.097 | 206 | 126 | 4 | 7 | 212 | 2 | 189 | 2.42e-21 | 92.8 |
| Msa0426600 | MtrunA17_Chr8g0347251 | 56.716 | 67 | 29 | 0 | 134 | 200 | 129 | 195 | 1.32e-20 | 90.9 |
| Msa0426600 | MtrunA17_Chr8g0387981 | 33.679 | 193 | 106 | 6 | 17 | 200 | 12 | 191 | 5.26e-20 | 89.4 |
| Msa0426600 | MtrunA17_Chr5g0431811 | 39.706 | 68 | 41 | 0 | 142 | 209 | 673 | 740 | 8.76e-13 | 69.3 |
| Msa0426600 | MtrunA17_Chr7g0224801 | 46.377 | 69 | 33 | 2 | 131 | 199 | 84 | 148 | 3.04e-12 | 64.3 |
| Msa0426600 | MtrunA17_Chr4g0071521 | 40.000 | 90 | 44 | 2 | 125 | 206 | 221 | 308 | 4.91e-12 | 65.9 |
| Msa0426600 | MtrunA17_Chr8g0387501 | 46.154 | 65 | 33 | 1 | 134 | 198 | 122 | 184 | 6.08e-12 | 65.1 |
| Msa0426600 | MtrunA17_Chr8g0348311 | 41.026 | 78 | 41 | 2 | 125 | 199 | 136 | 211 | 9.50e-12 | 65.1 |
| Msa0426600 | MtrunA17_Chr1g0147881 | 38.462 | 91 | 44 | 3 | 125 | 205 | 238 | 326 | 1.13e-11 | 64.7 |
| Msa0426600 | MtrunA17_Chr5g0418781 | 38.372 | 86 | 49 | 2 | 142 | 227 | 125 | 206 | 1.53e-11 | 63.9 |
| Msa0426600 | MtrunA17_Chr7g0238921 | 52.632 | 57 | 24 | 1 | 142 | 198 | 125 | 178 | 6.67e-11 | 62.4 |
| Msa0426600 | MtrunA17_Chr8g0386241 | 32.110 | 109 | 64 | 3 | 90 | 198 | 59 | 157 | 9.57e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0426600 | AT3G56400.1 | 44.144 | 222 | 106 | 5 | 13 | 234 | 7 | 210 | 1.48e-50 | 170 |
| Msa0426600 | AT2G40750.1 | 34.259 | 324 | 185 | 7 | 10 | 310 | 8 | 326 | 1.98e-46 | 160 |
| Msa0426600 | AT5G24110.1 | 34.715 | 193 | 89 | 7 | 13 | 200 | 12 | 172 | 6.41e-23 | 97.1 |
| Msa0426600 | AT2G46400.1 | 30.357 | 224 | 102 | 5 | 13 | 226 | 4 | 183 | 9.12e-22 | 93.6 |
| Msa0426600 | AT4G11070.1 | 31.280 | 211 | 121 | 7 | 13 | 208 | 7 | 208 | 1.15e-21 | 94.0 |
| Msa0426600 | AT1G66550.1 | 34.054 | 185 | 97 | 4 | 18 | 202 | 10 | 169 | 2.73e-21 | 91.7 |
| Msa0426600 | AT1G66550.2 | 34.595 | 185 | 91 | 6 | 18 | 202 | 10 | 164 | 6.29e-21 | 90.5 |
| Msa0426600 | AT1G66560.1 | 27.642 | 246 | 140 | 6 | 11 | 250 | 3 | 216 | 6.91e-20 | 87.8 |
| Msa0426600 | AT5G22570.1 | 28.704 | 216 | 114 | 4 | 18 | 217 | 11 | 202 | 7.50e-20 | 88.2 |
| Msa0426600 | AT1G66600.1 | 29.752 | 242 | 136 | 5 | 18 | 259 | 10 | 217 | 9.40e-20 | 87.0 |
| Msa0426600 | AT5G01900.1 | 32.973 | 185 | 100 | 4 | 18 | 201 | 9 | 170 | 3.13e-19 | 86.3 |
| Msa0426600 | AT4G11070.2 | 29.126 | 206 | 100 | 6 | 13 | 208 | 7 | 176 | 1.19e-18 | 84.7 |
| Msa0426600 | AT2G40740.1 | 44.186 | 86 | 42 | 1 | 142 | 221 | 174 | 259 | 2.37e-18 | 84.3 |
| Msa0426600 | AT2G40740.3 | 44.186 | 86 | 42 | 1 | 142 | 221 | 183 | 268 | 3.15e-18 | 84.0 |
| Msa0426600 | AT4G23810.1 | 50.704 | 71 | 34 | 1 | 142 | 211 | 159 | 229 | 5.31e-18 | 83.6 |
| Msa0426600 | AT5G45260.1 | 35.714 | 154 | 92 | 4 | 47 | 198 | 1117 | 1265 | 3.02e-16 | 80.1 |
| Msa0426600 | AT1G80590.1 | 32.432 | 185 | 77 | 5 | 18 | 202 | 10 | 146 | 1.15e-14 | 72.8 |
| Msa0426600 | AT4G23550.1 | 49.153 | 59 | 28 | 1 | 140 | 198 | 139 | 195 | 7.17e-12 | 65.9 |
| Msa0426600 | AT5G52830.1 | 41.772 | 79 | 40 | 2 | 125 | 199 | 145 | 221 | 7.28e-12 | 65.9 |
| Msa0426600 | AT5G13080.1 | 43.243 | 74 | 38 | 2 | 126 | 199 | 53 | 122 | 2.50e-11 | 61.2 |
| Msa0426600 | AT2G46130.1 | 42.029 | 69 | 35 | 2 | 140 | 206 | 29 | 94 | 2.56e-11 | 60.5 |
| Msa0426600 | AT4G01250.1 | 38.961 | 77 | 42 | 3 | 125 | 198 | 109 | 183 | 5.68e-11 | 62.8 |
| Msa0426600 | AT1G69310.4 | 30.216 | 139 | 78 | 5 | 96 | 229 | 97 | 221 | 8.43e-11 | 62.4 |
| Msa0426600 | AT1G69310.2 | 30.216 | 139 | 78 | 5 | 96 | 229 | 97 | 221 | 8.43e-11 | 62.4 |
| Msa0426600 | AT1G69310.1 | 30.216 | 139 | 78 | 5 | 96 | 229 | 97 | 221 | 8.43e-11 | 62.4 |
| Msa0426600 | AT1G30650.1 | 36.364 | 99 | 48 | 3 | 120 | 205 | 183 | 279 | 9.81e-11 | 62.8 |
Find 82 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATCTTGAAATTGATTT+TGG | 0.027971 | 3_2:+77025595 | Msa0426600:CDS |
| TATGTAATTTGGTACATTTC+TGG | 0.119421 | 3_2:-77025262 | None:intergenic |
| AACACTCATTTGCTATATTT+TGG | 0.147599 | 3_2:-77023488 | None:intergenic |
| TTGTTGGCAGTGACAGTTTC+AGG | 0.206708 | 3_2:-77023517 | None:intergenic |
| TGCAACCTTGGTCATGCTTC+CGG | 0.212153 | 3_2:-77025204 | None:intergenic |
| CATTCTCTGAAGATACTAAT+TGG | 0.227820 | 3_2:+77025335 | Msa0426600:CDS |
| TGATGTTACTGACAACTTAT+TGG | 0.245112 | 3_2:+77025501 | Msa0426600:CDS |
| GTATATGATGATCAAATTAA+TGG | 0.270264 | 3_2:+77025900 | Msa0426600:three_prime_UTR |
| GCAACCTTGGTCATGCTTCC+GGG | 0.281835 | 3_2:-77025203 | None:intergenic |
| TTCTATCAATTGTACCTGTT+TGG | 0.295646 | 3_2:-77025233 | None:intergenic |
| TTAAAATCAGACCACAAATT+AGG | 0.299410 | 3_2:-77025526 | None:intergenic |
| CAGAATCATGTGAATCATTC+AGG | 0.322565 | 3_2:+77023733 | Msa0426600:CDS |
| CATGTGAATCATTCAGGTTC+AGG | 0.330373 | 3_2:+77023739 | Msa0426600:CDS |
| CCTACATATAATATCATATA+TGG | 0.331659 | 3_2:-77025817 | None:intergenic |
| GTAAATGAAAGGAAAATATT+AGG | 0.347821 | 3_2:-77023349 | None:intergenic |
| AGTAGTACTGTCGTCGTTGT+TGG | 0.356608 | 3_2:-77025470 | None:intergenic |
| AAGAATCCTGTTTGTTCTGA+TGG | 0.357623 | 3_2:+77023607 | Msa0426600:CDS |
| GTCTCCTTCTGTTAAGGAAT+TGG | 0.359709 | 3_2:+77023630 | Msa0426600:CDS |
| GTTTGGTTGCTTTGCAACCT+TGG | 0.376612 | 3_2:-77025216 | None:intergenic |
| TAATTTGCAGGAGTTACTTC+AGG | 0.382346 | 3_2:+77025176 | Msa0426600:intron |
| GAAAGCCAATGTATGTAATT+TGG | 0.392701 | 3_2:-77025273 | None:intergenic |
| TGTTGGATTGGGTTAGGATT+TGG | 0.397596 | 3_2:-77023427 | None:intergenic |
| TCAGAGAATGAGACCATTTG+TGG | 0.397937 | 3_2:-77025322 | None:intergenic |
| TGATGGGTCTCCTTCTGTTA+AGG | 0.399624 | 3_2:+77023624 | Msa0426600:CDS |
| ACTCATAAAAGAGCTTCTTC+AGG | 0.399737 | 3_2:+77023549 | Msa0426600:CDS |
| TGTTGTTGTTGGATTGGGTT+AGG | 0.404346 | 3_2:-77023433 | None:intergenic |
| CAATTGGTGACACTCATTCC+TGG | 0.409828 | 3_2:+77024082 | Msa0426600:CDS |
| TAAAACTTACAAATGGTAAC+TGG | 0.414491 | 3_2:-77025790 | None:intergenic |
| ATTCTCTGAAGATACTAATT+GGG | 0.419258 | 3_2:+77025336 | Msa0426600:CDS |
| GTAGTACTGTCGTCGTTGTT+GGG | 0.426318 | 3_2:-77025469 | None:intergenic |
| CCTCTTCGATCCTTTGTAGT+TGG | 0.426598 | 3_2:-77023844 | None:intergenic |
| ACCATAGTCTCTAAAACAAT+TGG | 0.428053 | 3_2:+77024066 | Msa0426600:CDS |
| TTCAGGGGAACATGGTTCAT+TGG | 0.430865 | 3_2:+77023756 | Msa0426600:CDS |
| AGAATCCTGTTTGTTCTGAT+GGG | 0.439454 | 3_2:+77023608 | Msa0426600:CDS |
| ACCTTGGGAAATTAGAATTG+AGG | 0.441878 | 3_2:-77024128 | None:intergenic |
| TCCTTCTGTTAAGGAATTGG+TGG | 0.442137 | 3_2:+77023633 | Msa0426600:CDS |
| TAGTGGTGGAAGAAAAGAAA+AGG | 0.447168 | 3_2:-77023398 | None:intergenic |
| TTAGGATTTGGCAAATATAG+TGG | 0.458785 | 3_2:-77023415 | None:intergenic |
| TGCAATGAAACTAAAGGTTG+TGG | 0.465134 | 3_2:+77025934 | Msa0426600:three_prime_UTR |
| AGACCATTTGTGGAGTTTGT+AGG | 0.471099 | 3_2:-77025312 | None:intergenic |
| GTAGGCGTGTGCATATATAA+AGG | 0.478171 | 3_2:+77025835 | Msa0426600:three_prime_UTR |
| CTTCTGACCGTACTTTCTCC+AGG | 0.478180 | 3_2:-77024100 | None:intergenic |
| ACTTATTGGATCCTAATTTG+TGG | 0.485951 | 3_2:+77025515 | Msa0426600:CDS |
| CACCCTACAAACTCCACAAA+TGG | 0.486229 | 3_2:+77025309 | Msa0426600:CDS |
| ATGAAAGGAAAATATTAGGA+AGG | 0.498685 | 3_2:-77023345 | None:intergenic |
| ACAAAATGCAATGAAACTAA+AGG | 0.505426 | 3_2:+77025928 | Msa0426600:three_prime_UTR |
| ATGTGAATCATTCAGGTTCA+GGG | 0.513638 | 3_2:+77023740 | Msa0426600:CDS |
| CTCATAAAAGAGCTTCTTCA+GGG | 0.513650 | 3_2:+77023550 | Msa0426600:CDS |
| CATTCAGGTTCAGGGGAACA+TGG | 0.514743 | 3_2:+77023748 | Msa0426600:CDS |
| GCAGGAATACACAATATCCT+TGG | 0.514816 | 3_2:-77025563 | None:intergenic |
| TGGCAGATAAAACTTACAAA+TGG | 0.519708 | 3_2:-77025797 | None:intergenic |
| AGGTTGCAAAGCAACCAAAC+AGG | 0.523803 | 3_2:+77025219 | Msa0426600:CDS |
| GACCATTTGTGGAGTTTGTA+GGG | 0.524922 | 3_2:-77025311 | None:intergenic |
| AGTGGTGGAAGAAAAGAAAA+GGG | 0.528613 | 3_2:-77023397 | None:intergenic |
| AGGGGAACATGGTTCATTGG+TGG | 0.531988 | 3_2:+77023759 | Msa0426600:CDS |
| GGAGACCCATCAGAACAAAC+AGG | 0.533380 | 3_2:-77023613 | None:intergenic |
| GCAATGAAACTAAAGGTTGT+GGG | 0.536305 | 3_2:+77025935 | Msa0426600:three_prime_UTR |
| CACATGTTATTAGCTCAGAA+AGG | 0.537868 | 3_2:+77025425 | Msa0426600:CDS |
| GAAGAGGTTCCTACAAAAGA+AGG | 0.538026 | 3_2:+77023860 | Msa0426600:CDS |
| CCATATATGATATTATATGT+AGG | 0.552215 | 3_2:+77025817 | Msa0426600:three_prime_UTR |
| TGAAAGGAAAATATTAGGAA+GGG | 0.553632 | 3_2:-77023344 | None:intergenic |
| GCCACCAATTCCTTAACAGA+AGG | 0.556097 | 3_2:-77023634 | None:intergenic |
| TGAAGATTGTAGTTTAGATG+AGG | 0.565987 | 3_2:+77023705 | Msa0426600:CDS |
| ATGTACCAAATTACATACAT+TGG | 0.567198 | 3_2:+77025268 | Msa0426600:CDS |
| CTGGAGAAAGTACGGTCAGA+AGG | 0.567429 | 3_2:+77024101 | Msa0426600:CDS |
| TCCTCAATTCTAATTTCCCA+AGG | 0.568294 | 3_2:+77024127 | Msa0426600:CDS |
| GTCAGAACCTCCTTTGAGTG+AGG | 0.572819 | 3_2:-77025382 | None:intergenic |
| GAAAGTGGGATGTAAATGAA+AGG | 0.574023 | 3_2:-77023360 | None:intergenic |
| GGAGTTACTTCAGGTGCACC+CGG | 0.575709 | 3_2:+77025185 | Msa0426600:intron |
| ACTCATTCCTGGAGAAAGTA+CGG | 0.586004 | 3_2:+77024093 | Msa0426600:CDS |
| GAGATTGTCGCCAACTACAA+AGG | 0.592832 | 3_2:+77023834 | Msa0426600:CDS |
| GGATTTGGCAAATATAGTGG+TGG | 0.605725 | 3_2:-77023412 | None:intergenic |
| GAATTCTAATCCTCACTCAA+AGG | 0.633382 | 3_2:+77025372 | Msa0426600:CDS |
| CATTTGTGGAGTTTGTAGGG+TGG | 0.641904 | 3_2:-77025308 | None:intergenic |
| TTCTAATCCTCACTCAAAGG+AGG | 0.641909 | 3_2:+77025375 | Msa0426600:CDS |
| TTGTCAGTAACATCACTCCT+CGG | 0.646183 | 3_2:-77025493 | None:intergenic |
| TGTGAATCATTCAGGTTCAG+GGG | 0.655461 | 3_2:+77023741 | Msa0426600:CDS |
| TGCACCCGGAAGCATGACCA+AGG | 0.656558 | 3_2:+77025199 | Msa0426600:CDS |
| GTACATCAAATTGACAGACG+TGG | 0.690360 | 3_2:-77025716 | None:intergenic |
| GCAGGAAAACAGATGAGACA+AGG | 0.691983 | 3_2:+77024043 | Msa0426600:intron |
| CCAACTACAAAGGATCGAAG+AGG | 0.701163 | 3_2:+77023844 | Msa0426600:CDS |
| ACGACGACAGTACTACTCCG+AGG | 0.763721 | 3_2:+77025476 | Msa0426600:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATATTTATTAAAATTGATT+TGG | + | chr3_2:77024395-77024414 | Msa0426600:intron | 10.0% |
| !!! | AAATTTTACACTTAAATTTA+TGG | + | chr3_2:77024476-77024495 | Msa0426600:intron | 10.0% |
| !! | AAAAAAAAAAACTAATTAGT+GGG | - | chr3_2:77024999-77025018 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAACTAATTAG+TGG | - | chr3_2:77025000-77025019 | None:intergenic | 10.0% |
| !! | TTAAGTGTAAAATTTACTTT+GGG | - | chr3_2:77024471-77024490 | None:intergenic | 15.0% |
| !! | TTTAAGTGTAAAATTTACTT+TGG | - | chr3_2:77024472-77024491 | None:intergenic | 15.0% |
| !! | ATATAATCTCATTTCAAAAT+CGG | - | chr3_2:77024955-77024974 | None:intergenic | 15.0% |
| !!! | AAAATCTTGAAATTGATTTT+GGG | + | chr3_2:77025596-77025615 | Msa0426600:CDS | 15.0% |
| !! | GTAAATGAAAGGAAAATATT+AGG | - | chr3_2:77023352-77023371 | None:intergenic | 20.0% |
| !! | TAGAAAACAAAAAAATCAAC+AGG | - | chr3_2:77023957-77023976 | None:intergenic | 20.0% |
| !! | TTTATTCTTAATTATTCTGC+AGG | + | chr3_2:77024025-77024044 | Msa0426600:intron | 20.0% |
| !! | TTAGATGTGTATAATTGATT+TGG | + | chr3_2:77024360-77024379 | Msa0426600:intron | 20.0% |
| !! | TAAGTGTAAAATTTACTTTG+GGG | - | chr3_2:77024470-77024489 | None:intergenic | 20.0% |
| !! | TTACACTTAAATTTATGGTT+AGG | + | chr3_2:77024481-77024500 | Msa0426600:intron | 20.0% |
| !! | AACTATAAAGTCTATACAAA+AGG | - | chr3_2:77024870-77024889 | None:intergenic | 20.0% |
| !! | TTATAGTTTCAACAAAATCA+TGG | + | chr3_2:77024882-77024901 | Msa0426600:intron | 20.0% |
| !! | GAATTGATTAACTAATTAGT+GGG | - | chr3_2:77025037-77025056 | None:intergenic | 20.0% |
| !! | TGAATTGATTAACTAATTAG+TGG | - | chr3_2:77025038-77025057 | None:intergenic | 20.0% |
| !!! | CAAAATCTTGAAATTGATTT+TGG | + | chr3_2:77025595-77025614 | Msa0426600:CDS | 20.0% |
| !!! | AAATCTTGAAATTGATTTTG+GGG | + | chr3_2:77025597-77025616 | Msa0426600:CDS | 20.0% |
| !! | CCTACATATAATATCATATA+TGG | - | chr3_2:77025820-77025839 | None:intergenic | 20.0% |
| !! | CCATATATGATATTATATGT+AGG | + | chr3_2:77025817-77025836 | Msa0426600:three_prime_UTR | 20.0% |
| !! | GTATATGATGATCAAATTAA+TGG | + | chr3_2:77025900-77025919 | Msa0426600:three_prime_UTR | 20.0% |
| ! | TGAAAGGAAAATATTAGGAA+GGG | - | chr3_2:77023347-77023366 | None:intergenic | 25.0% |
| ! | ATGAAAGGAAAATATTAGGA+AGG | - | chr3_2:77023348-77023367 | None:intergenic | 25.0% |
| !!! | AACACTCATTTGCTATATTT+TGG | - | chr3_2:77023491-77023510 | None:intergenic | 25.0% |
| !! | AAAATAGTACCTTCTTTTGT+AGG | - | chr3_2:77023872-77023891 | None:intergenic | 25.0% |
| !!! | AAGAAGGTACTATTTTTGTA+CGG | + | chr3_2:77023876-77023895 | Msa0426600:intron | 25.0% |
| ! | AGGAAAATAATGATTACCTT+GGG | - | chr3_2:77024146-77024165 | None:intergenic | 25.0% |
| ! | ACTTGCTCATATTGTATAAA+TGG | - | chr3_2:77024228-77024247 | None:intergenic | 25.0% |
| !!! | AGATTATAGAAGCATTTTAG+AGG | + | chr3_2:77024332-77024351 | Msa0426600:intron | 25.0% |
| ! | TCAACTCTTACAAAATCAAT+TGG | + | chr3_2:77024572-77024591 | Msa0426600:intron | 25.0% |
| ! | TGAGATTATATACTATCAAG+TGG | + | chr3_2:77024964-77024983 | Msa0426600:intron | 25.0% |
| ! | TACTAGTATACAAATTAACC+CGG | + | chr3_2:77025074-77025093 | Msa0426600:intron | 25.0% |
| ! | TATGTAATTTGGTACATTTC+TGG | - | chr3_2:77025265-77025284 | None:intergenic | 25.0% |
| ! | ATGTACCAAATTACATACAT+TGG | + | chr3_2:77025268-77025287 | Msa0426600:CDS | 25.0% |
| !! | ATTCTCTGAAGATACTAATT+GGG | + | chr3_2:77025336-77025355 | Msa0426600:CDS | 25.0% |
| ! | TTAAAATCAGACCACAAATT+AGG | - | chr3_2:77025529-77025548 | None:intergenic | 25.0% |
| !!! | TAATTTGTGGTCTGATTTTA+AGG | + | chr3_2:77025528-77025547 | Msa0426600:CDS | 25.0% |
| ! | TAAAACTTACAAATGGTAAC+TGG | - | chr3_2:77025793-77025812 | None:intergenic | 25.0% |
| !!! | TTTAGTTTCATTGCATTTTG+TGG | - | chr3_2:77025929-77025948 | None:intergenic | 25.0% |
| ! | ACAAAATGCAATGAAACTAA+AGG | + | chr3_2:77025928-77025947 | Msa0426600:three_prime_UTR | 25.0% |
| !! | TATGTTTTTGCTAGGAAAGT+GGG | - | chr3_2:77023377-77023396 | None:intergenic | 30.0% |
| TTAGGATTTGGCAAATATAG+TGG | - | chr3_2:77023418-77023437 | None:intergenic | 30.0% | |
| !!! | TTTGCTATATTTTGGTGTAG+TGG | - | chr3_2:77023483-77023502 | None:intergenic | 30.0% |
| ! | TATGAGTAGCTTCTTTTTGT+TGG | - | chr3_2:77023536-77023555 | None:intergenic | 30.0% |
| TGAAGATTGTAGTTTAGATG+AGG | + | chr3_2:77023705-77023724 | Msa0426600:CDS | 30.0% | |
| ! | CATGTTTTCTTTTTCCTTGT+TGG | + | chr3_2:77023910-77023929 | Msa0426600:intron | 30.0% |
| ! | GTTTTCATTTCATTCCAACA+AGG | - | chr3_2:77023927-77023946 | None:intergenic | 30.0% |
| TTGGAATGAAATGAAAACGT+GGG | + | chr3_2:77023929-77023948 | Msa0426600:intron | 30.0% | |
| !! | ACCATAGTCTCTAAAACAAT+TGG | + | chr3_2:77024066-77024085 | Msa0426600:CDS | 30.0% |
| !!! | ACCAATTGTTTTAGAGACTA+TGG | - | chr3_2:77024070-77024089 | None:intergenic | 30.0% |
| GAGGAAAATAATGATTACCT+TGG | - | chr3_2:77024147-77024166 | None:intergenic | 30.0% | |
| AAATCAGTAGAAAAAAGCAG+AGG | - | chr3_2:77024166-77024185 | None:intergenic | 30.0% | |
| !! | TATGGTTAGGTTCACTTTTA+CGG | + | chr3_2:77024494-77024513 | Msa0426600:intron | 30.0% |
| ATGTGCATGTTTCAATATCA+TGG | + | chr3_2:77024621-77024640 | Msa0426600:intron | 30.0% | |
| !! | CACAGTTGGCAAATTTTAAT+TGG | - | chr3_2:77024802-77024821 | None:intergenic | 30.0% |
| !!! | AGAATTTTTCAAGACACAGT+TGG | - | chr3_2:77024816-77024835 | None:intergenic | 30.0% |
| ACTAGTATACAAATTAACCC+GGG | + | chr3_2:77025075-77025094 | Msa0426600:intron | 30.0% | |
| CCTGCAAATTATCATTGAAA+AGG | - | chr3_2:77025167-77025186 | None:intergenic | 30.0% | |
| !! | CCTTTTCAATGATAATTTGC+AGG | + | chr3_2:77025164-77025183 | Msa0426600:intron | 30.0% |
| TTCTATCAATTGTACCTGTT+TGG | - | chr3_2:77025236-77025255 | None:intergenic | 30.0% | |
| GAAAGCCAATGTATGTAATT+TGG | - | chr3_2:77025276-77025295 | None:intergenic | 30.0% | |
| ! | CATTCTCTGAAGATACTAAT+TGG | + | chr3_2:77025335-77025354 | Msa0426600:CDS | 30.0% |
| TGATGTTACTGACAACTTAT+TGG | + | chr3_2:77025501-77025520 | Msa0426600:CDS | 30.0% | |
| ACTTATTGGATCCTAATTTG+TGG | + | chr3_2:77025515-77025534 | Msa0426600:CDS | 30.0% | |
| TGGCAGATAAAACTTACAAA+TGG | - | chr3_2:77025800-77025819 | None:intergenic | 30.0% | |
| GAAAGTGGGATGTAAATGAA+AGG | - | chr3_2:77023363-77023382 | None:intergenic | 35.0% | |
| !! | GTATGTTTTTGCTAGGAAAG+TGG | - | chr3_2:77023378-77023397 | None:intergenic | 35.0% |
| !! | GAAAAGGGTATGTTTTTGCT+AGG | - | chr3_2:77023385-77023404 | None:intergenic | 35.0% |
| ! | AGTGGTGGAAGAAAAGAAAA+GGG | - | chr3_2:77023400-77023419 | None:intergenic | 35.0% |
| ! | TAGTGGTGGAAGAAAAGAAA+AGG | - | chr3_2:77023401-77023420 | None:intergenic | 35.0% |
| ! | GTTGTTGTTGTTGTTGGATT+GGG | - | chr3_2:77023441-77023460 | None:intergenic | 35.0% |
| ! | TGTTGTTGTTGTTGTTGGAT+TGG | - | chr3_2:77023442-77023461 | None:intergenic | 35.0% |
| ! | TGTGTTGTTGTTGTTGTTGT+TGG | - | chr3_2:77023447-77023466 | None:intergenic | 35.0% |
| !!! | ATATTTTGGTGTAGTGGATC+AGG | - | chr3_2:77023477-77023496 | None:intergenic | 35.0% |
| ACTCATAAAAGAGCTTCTTC+AGG | + | chr3_2:77023549-77023568 | Msa0426600:CDS | 35.0% | |
| CTCATAAAAGAGCTTCTTCA+GGG | + | chr3_2:77023550-77023569 | Msa0426600:CDS | 35.0% | |
| AAGAATCCTGTTTGTTCTGA+TGG | + | chr3_2:77023607-77023626 | Msa0426600:CDS | 35.0% | |
| AGAATCCTGTTTGTTCTGAT+GGG | + | chr3_2:77023608-77023627 | Msa0426600:CDS | 35.0% | |
| CAGAATCATGTGAATCATTC+AGG | + | chr3_2:77023733-77023752 | Msa0426600:CDS | 35.0% | |
| ! | ATGTGAATCATTCAGGTTCA+GGG | + | chr3_2:77023740-77023759 | Msa0426600:CDS | 35.0% |
| !!! | ACTATTTTTGTACGGACACA+AGG | + | chr3_2:77023884-77023903 | Msa0426600:intron | 35.0% |
| GTTGGAATGAAATGAAAACG+TGG | + | chr3_2:77023928-77023947 | Msa0426600:intron | 35.0% | |
| TCCTCAATTCTAATTTCCCA+AGG | + | chr3_2:77024127-77024146 | Msa0426600:CDS | 35.0% | |
| ACCTTGGGAAATTAGAATTG+AGG | - | chr3_2:77024131-77024150 | None:intergenic | 35.0% | |
| ATAGCAGATGTTTGGTTATG+CGG | - | chr3_2:77024603-77024622 | None:intergenic | 35.0% | |
| GGCAAATAATGCATTGTGAT+TGG | - | chr3_2:77024729-77024748 | None:intergenic | 35.0% | |
| GCATTATTTGCCACAATTCA+AGG | + | chr3_2:77024737-77024756 | Msa0426600:intron | 35.0% | |
| TTATTTGCCACAATTCAAGG+AGG | + | chr3_2:77024740-77024759 | Msa0426600:intron | 35.0% | |
| ! | TAATTTGCAGGAGTTACTTC+AGG | + | chr3_2:77025176-77025195 | Msa0426600:intron | 35.0% |
| GAATTCTAATCCTCACTCAA+AGG | + | chr3_2:77025372-77025391 | Msa0426600:CDS | 35.0% | |
| CACATGTTATTAGCTCAGAA+AGG | + | chr3_2:77025425-77025444 | Msa0426600:CDS | 35.0% | |
| ! | TAAGGATTTTGAACTCTCCA+AGG | + | chr3_2:77025546-77025565 | Msa0426600:CDS | 35.0% |
| !!! | AAGATTTTGAGATTCAGTGC+AGG | - | chr3_2:77025584-77025603 | None:intergenic | 35.0% |
| ! | AGTTTCATTGCATTTTGTGG+AGG | - | chr3_2:77025926-77025945 | None:intergenic | 35.0% |
| GGATTTGGCAAATATAGTGG+TGG | - | chr3_2:77023415-77023434 | None:intergenic | 40.0% | |
| ! | TGTTGGATTGGGTTAGGATT+TGG | - | chr3_2:77023430-77023449 | None:intergenic | 40.0% |
| ! | TGTTGTTGTTGGATTGGGTT+AGG | - | chr3_2:77023436-77023455 | None:intergenic | 40.0% |
| GTCTCCTTCTGTTAAGGAAT+TGG | + | chr3_2:77023630-77023649 | Msa0426600:CDS | 40.0% | |
| TCCTTCTGTTAAGGAATTGG+TGG | + | chr3_2:77023633-77023652 | Msa0426600:CDS | 40.0% | |
| ! | CATGTGAATCATTCAGGTTC+AGG | + | chr3_2:77023739-77023758 | Msa0426600:CDS | 40.0% |
| ! | TGTGAATCATTCAGGTTCAG+GGG | + | chr3_2:77023741-77023760 | Msa0426600:CDS | 40.0% |
| GAAGAGGTTCCTACAAAAGA+AGG | + | chr3_2:77023860-77023879 | Msa0426600:CDS | 40.0% | |
| ACTCATTCCTGGAGAAAGTA+CGG | + | chr3_2:77024093-77024112 | Msa0426600:CDS | 40.0% | |
| TAGCAGATGTTTGGTTATGC+GGG | - | chr3_2:77024602-77024621 | None:intergenic | 40.0% | |
| ACATGCACATAGCAGATGTT+TGG | - | chr3_2:77024611-77024630 | None:intergenic | 40.0% | |
| TGTGGCTGAAAAATAGGAAG+TGG | + | chr3_2:77024763-77024782 | Msa0426600:intron | 40.0% | |
| GACCATTTGTGGAGTTTGTA+GGG | - | chr3_2:77025314-77025333 | None:intergenic | 40.0% | |
| AGACCATTTGTGGAGTTTGT+AGG | - | chr3_2:77025315-77025334 | None:intergenic | 40.0% | |
| TCAGAGAATGAGACCATTTG+TGG | - | chr3_2:77025325-77025344 | None:intergenic | 40.0% | |
| TTCTAATCCTCACTCAAAGG+AGG | + | chr3_2:77025375-77025394 | Msa0426600:CDS | 40.0% | |
| TTGTCAGTAACATCACTCCT+CGG | - | chr3_2:77025496-77025515 | None:intergenic | 40.0% | |
| GCAGGAATACACAATATCCT+TGG | - | chr3_2:77025566-77025585 | None:intergenic | 40.0% | |
| GTACATCAAATTGACAGACG+TGG | - | chr3_2:77025719-77025738 | None:intergenic | 40.0% | |
| GTAGGCGTGTGCATATATAA+AGG | + | chr3_2:77025835-77025854 | Msa0426600:three_prime_UTR | 40.0% | |
| TTGTTGGCAGTGACAGTTTC+AGG | - | chr3_2:77023520-77023539 | None:intergenic | 45.0% | |
| ! | TGATGGGTCTCCTTCTGTTA+AGG | + | chr3_2:77023624-77023643 | Msa0426600:CDS | 45.0% |
| GCCACCAATTCCTTAACAGA+AGG | - | chr3_2:77023637-77023656 | None:intergenic | 45.0% | |
| TTCAGGGGAACATGGTTCAT+TGG | + | chr3_2:77023756-77023775 | Msa0426600:CDS | 45.0% | |
| GAGATTGTCGCCAACTACAA+AGG | + | chr3_2:77023834-77023853 | Msa0426600:CDS | 45.0% | |
| CCTCTTCGATCCTTTGTAGT+TGG | - | chr3_2:77023847-77023866 | None:intergenic | 45.0% | |
| CCAACTACAAAGGATCGAAG+AGG | + | chr3_2:77023844-77023863 | Msa0426600:CDS | 45.0% | |
| GCAGGAAAACAGATGAGACA+AGG | + | chr3_2:77024043-77024062 | Msa0426600:intron | 45.0% | |
| ! | CAATTGGTGACACTCATTCC+TGG | + | chr3_2:77024082-77024101 | Msa0426600:CDS | 45.0% |
| ! | AGGAGGTGTGGCTGAAAAAT+AGG | + | chr3_2:77024757-77024776 | Msa0426600:intron | 45.0% |
| AAACACTATACCAGCTAGCC+CGG | - | chr3_2:77025096-77025115 | None:intergenic | 45.0% | |
| ! | GTTTGGTTGCTTTGCAACCT+TGG | - | chr3_2:77025219-77025238 | None:intergenic | 45.0% |
| AGGTTGCAAAGCAACCAAAC+AGG | + | chr3_2:77025219-77025238 | Msa0426600:CDS | 45.0% | |
| CATTTGTGGAGTTTGTAGGG+TGG | - | chr3_2:77025311-77025330 | None:intergenic | 45.0% | |
| CACCCTACAAACTCCACAAA+TGG | + | chr3_2:77025309-77025328 | Msa0426600:CDS | 45.0% | |
| GTAGTACTGTCGTCGTTGTT+GGG | - | chr3_2:77025472-77025491 | None:intergenic | 45.0% | |
| AGTAGTACTGTCGTCGTTGT+TGG | - | chr3_2:77025473-77025492 | None:intergenic | 45.0% | |
| !!! | TAGTAAAAAATATTTTTTAA+CGG | + | chr3_2:77024289-77024308 | Msa0426600:intron | 5.0% |
| GGAGACCCATCAGAACAAAC+AGG | - | chr3_2:77023616-77023635 | None:intergenic | 50.0% | |
| ! | CATTCAGGTTCAGGGGAACA+TGG | + | chr3_2:77023748-77023767 | Msa0426600:CDS | 50.0% |
| AGGGGAACATGGTTCATTGG+TGG | + | chr3_2:77023759-77023778 | Msa0426600:CDS | 50.0% | |
| CTTCTGACCGTACTTTCTCC+AGG | - | chr3_2:77024103-77024122 | None:intergenic | 50.0% | |
| ! | CTGGAGAAAGTACGGTCAGA+AGG | + | chr3_2:77024101-77024120 | Msa0426600:CDS | 50.0% |
| TGCCACAATTCAAGGAGGTG+TGG | + | chr3_2:77024745-77024764 | Msa0426600:intron | 50.0% | |
| AGCCACACCTCCTTGAATTG+TGG | - | chr3_2:77024750-77024769 | None:intergenic | 50.0% | |
| !! | ACAAATTAACCCGGGCTAGC+TGG | + | chr3_2:77025083-77025102 | Msa0426600:intron | 50.0% |
| AACACTATACCAGCTAGCCC+GGG | - | chr3_2:77025095-77025114 | None:intergenic | 50.0% | |
| TGCAACCTTGGTCATGCTTC+CGG | - | chr3_2:77025207-77025226 | None:intergenic | 50.0% | |
| GTCAGAACCTCCTTTGAGTG+AGG | - | chr3_2:77025385-77025404 | None:intergenic | 50.0% | |
| GGAGTTACTTCAGGTGCACC+CGG | + | chr3_2:77025185-77025204 | Msa0426600:intron | 55.0% | |
| GCAACCTTGGTCATGCTTCC+GGG | - | chr3_2:77025206-77025225 | None:intergenic | 55.0% | |
| ACGACGACAGTACTACTCCG+AGG | + | chr3_2:77025476-77025495 | Msa0426600:CDS | 55.0% | |
| ! | TGCACCCGGAAGCATGACCA+AGG | + | chr3_2:77025199-77025218 | Msa0426600:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 77023317 | 77025956 | 77023317 | ID=Msa0426600;Name=Msa0426600 |
| chr3_2 | mRNA | 77023317 | 77025956 | 77023317 | ID=Msa0426600-mRNA-1;Parent=Msa0426600;Name=Msa0426600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=185|1|1|1|1|1|3|278|324 |
| chr3_2 | exon | 77023317 | 77023881 | 77023317 | ID=Msa0426600-mRNA-1:exon:20019;Parent=Msa0426600-mRNA-1 |
| chr3_2 | exon | 77024047 | 77024148 | 77024047 | ID=Msa0426600-mRNA-1:exon:20020;Parent=Msa0426600-mRNA-1 |
| chr3_2 | exon | 77025186 | 77025956 | 77025186 | ID=Msa0426600-mRNA-1:exon:20021;Parent=Msa0426600-mRNA-1 |
| chr3_2 | five_prime_UTR | 77023317 | 77023501 | 77023317 | ID=Msa0426600-mRNA-1:five_prime_utr;Parent=Msa0426600-mRNA-1 |
| chr3_2 | CDS | 77023502 | 77023881 | 77023502 | ID=Msa0426600-mRNA-1:cds;Parent=Msa0426600-mRNA-1 |
| chr3_2 | CDS | 77024047 | 77024148 | 77024047 | ID=Msa0426600-mRNA-1:cds;Parent=Msa0426600-mRNA-1 |
| chr3_2 | CDS | 77025186 | 77025678 | 77025186 | ID=Msa0426600-mRNA-1:cds;Parent=Msa0426600-mRNA-1 |
| chr3_2 | three_prime_UTR | 77025679 | 77025956 | 77025679 | ID=Msa0426600-mRNA-1:three_prime_utr;Parent=Msa0426600-mRNA-1 |
| Gene Sequence |
| Protein sequence |
