Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0429070 | XP_003602892.1 | 100.000 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 8.54e-155 | 438 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0429070 | G7J4K7 | 100.000 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 4.08e-155 | 438 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0027540 | Msa0429070 | -0.808253 | 3.392046e-50 | -8.615850e-47 |
| Msa0075310 | Msa0429070 | -0.811391 | 7.166377e-51 | -8.615850e-47 |
| Msa0375080 | Msa0429070 | 0.815911 | 7.250928e-52 | -8.615850e-47 |
| Msa0378340 | Msa0429070 | -0.825976 | 3.494521e-54 | -8.615850e-47 |
| Msa0419620 | Msa0429070 | 0.804171 | 2.455470e-49 | -8.615850e-47 |
| Msa0423360 | Msa0429070 | 0.800744 | 1.248446e-48 | -8.615850e-47 |
| Msa0427520 | Msa0429070 | 0.819219 | 1.302939e-52 | -8.615850e-47 |
| Msa0427690 | Msa0429070 | -0.824792 | 6.661930e-54 | -8.615850e-47 |
| Msa0429050 | Msa0429070 | 0.957981 | 7.256731e-116 | -8.615850e-47 |
| Msa0429070 | Msa0473940 | 0.823473 | 1.359056e-53 | -8.615850e-47 |
| Msa0429070 | Msa0511720 | 0.815739 | 7.922689e-52 | -8.615850e-47 |
| Msa0429070 | Msa0518340 | 0.803749 | 3.004944e-49 | -8.615850e-47 |
| Msa0429070 | Msa0518520 | -0.825599 | 4.292879e-54 | -8.615850e-47 |
| Msa0429070 | Msa0524120 | -0.805911 | 1.061840e-49 | -8.615850e-47 |
| Msa0429070 | Msa0616910 | -0.811766 | 5.940355e-51 | -8.615850e-47 |
| Msa0429070 | Msa0642910 | -0.800723 | 1.260493e-48 | -8.615850e-47 |
| Msa0429070 | Msa0687000 | -0.802324 | 5.922789e-49 | -8.615850e-47 |
| Msa0429070 | Msa0694690 | 0.803313 | 3.699936e-49 | -8.615850e-47 |
| Msa0429070 | Msa0705550 | -0.809287 | 2.038968e-50 | -8.615850e-47 |
| Msa0429070 | Msa0725480 | 0.838344 | 3.041376e-57 | -8.615850e-47 |
| Msa0429070 | Msa0766100 | 0.849438 | 3.233140e-60 | -8.615850e-47 |
| Msa0429070 | Msa0877420 | -0.802707 | 4.936247e-49 | -8.615850e-47 |
| Msa0429070 | Msa0951710 | -0.800775 | 1.230216e-48 | -8.615850e-47 |
| Msa0429070 | Msa0979550 | -0.817317 | 3.511452e-52 | -8.615850e-47 |
| Msa0429070 | Msa1037060 | 0.822850 | 1.899904e-53 | -8.615850e-47 |
| Msa0429070 | Msa1164800 | 0.819881 | 9.199597e-53 | -8.615850e-47 |
| Msa0429070 | Msa1220450 | 0.814603 | 1.416483e-51 | -8.615850e-47 |
| Msa0429070 | Msa1220460 | 0.819110 | 1.379046e-52 | -8.615850e-47 |
| Msa0429070 | Msa1220480 | 0.818918 | 1.525459e-52 | -8.615850e-47 |
| Msa0429070 | Msa1221570 | 0.816039 | 6.792036e-52 | -8.615850e-47 |
| Msa0429070 | Msa1305740 | -0.814161 | 1.773485e-51 | -8.615850e-47 |
| Msa0429070 | Msa1359080 | 0.816146 | 6.428169e-52 | -8.615850e-47 |
| Msa0429070 | Msa1370910 | 0.830113 | 3.525535e-55 | -8.615850e-47 |
| Msa0429070 | Msa1371320 | 0.806458 | 8.146364e-50 | -8.615850e-47 |
| Msa0429070 | Msa1382720 | -0.825818 | 3.808659e-54 | -8.615850e-47 |
| Msa0429070 | Msa1391360 | 0.818930 | 1.515437e-52 | -8.615850e-47 |
| Msa0429070 | Msa1425010 | -0.806602 | 7.594855e-50 | -8.615850e-47 |
| Msa0257930 | Msa0429070 | 0.815961 | 7.070210e-52 | -8.615850e-47 |
| Msa0263620 | Msa0429070 | -0.800230 | 1.588775e-48 | -8.615850e-47 |
| Msa0293860 | Msa0429070 | 0.805392 | 1.364526e-49 | -8.615850e-47 |
| Msa0320540 | Msa0429070 | -0.801323 | 9.507045e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0429070 | MtrunA17_Chr3g0132801 | 100.000 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 7.85e-159 | 438 |
| Msa0429070 | MtrunA17_Chr8g0367261 | 37.981 | 208 | 119 | 3 | 18 | 220 | 13 | 215 | 7.58e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0429070 | AT5G65520.1 | 41.146 | 192 | 108 | 3 | 18 | 207 | 12 | 200 | 3.90e-40 | 137 |
Find 44 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATACGAAGCGGCGTCTTT+TGG | 0.244593 | 3_2:-80241775 | Msa0429050:intron |
| CACCAAGCTTTCGCGTTCTT+CGG | 0.307624 | 3_2:-80242009 | Msa0429050:intron |
| GGAAGCAAAGAAAGCTTATA+AGG | 0.314616 | 3_2:+80242071 | Msa0429070:CDS |
| GTGTTATGAAGGGAAGAAAA+TGG | 0.346267 | 3_2:+80242044 | Msa0429070:CDS |
| TTCGTTTGCGAGGTTGTTGA+TGG | 0.363091 | 3_2:-80241716 | Msa0429050:intron |
| TGCCGGTAGAAGCACTTAAC+AGG | 0.406267 | 3_2:+80242124 | Msa0429070:CDS |
| GAGGTTGTTGATGGCGGATC+TGG | 0.441588 | 3_2:-80241707 | Msa0429050:intron |
| GTCCGAAGAACGCGAAAGCT+TGG | 0.448178 | 3_2:+80242007 | Msa0429070:CDS |
| GCGAAAGCTTGGTGTGCGTT+GGG | 0.450050 | 3_2:+80242018 | Msa0429070:CDS |
| CGCGAAAGCTTGGTGTGCGT+TGG | 0.456259 | 3_2:+80242017 | Msa0429070:CDS |
| TTATAAGGAAGCTCATGAAT+TGG | 0.465517 | 3_2:+80242086 | Msa0429070:CDS |
| TTCACCTTGCTCAAGCGAAC+CGG | 0.466250 | 3_2:-80241881 | Msa0429050:intron |
| GCTATGATTCTCGATCTTCA+AGG | 0.478244 | 3_2:+80241805 | Msa0429070:CDS |
| GTAGAAGCACTTAACAGGTT+GGG | 0.489020 | 3_2:+80242129 | Msa0429070:CDS |
| ACGAGTTGACTCGGCGTTAG+CGG | 0.514403 | 3_2:+80241960 | Msa0429070:CDS |
| CGCCTAAGGCTTCGTTTGCG+AGG | 0.524088 | 3_2:-80241726 | Msa0429050:intron |
| TTAATCTTCCTCAGCATGCA+CGG | 0.553368 | 3_2:+80241565 | Msa0429070:CDS |
| GCGTTGTCTCCTCTCGCCGC+CGG | 0.558332 | 3_2:+80241862 | Msa0429070:CDS |
| GCGTTGGGAGAGTGTTATGA+AGG | 0.558916 | 3_2:+80242033 | Msa0429070:CDS |
| GGTAGAAGCACTTAACAGGT+TGG | 0.564746 | 3_2:+80242128 | Msa0429070:CDS |
| TGAACGCGGACGAGTTGACT+CGG | 0.566518 | 3_2:+80241951 | Msa0429070:CDS |
| CAAGCGATGTACCGCGACAT+CGG | 0.569247 | 3_2:+80241511 | Msa0429070:CDS |
| TGAAGGGAAGAAAATGGATG+AGG | 0.575977 | 3_2:+80242050 | Msa0429070:CDS |
| GGCGCCACAGTATTCTGTGC+CGG | 0.576837 | 3_2:+80242107 | Msa0429070:CDS |
| GCCGCCGGTTCGCTTGAGCA+AGG | 0.596105 | 3_2:+80241877 | Msa0429070:CDS |
| TTTGGAATCGGAGTTTAGCG+AGG | 0.596238 | 3_2:-80241603 | Msa0429050:intron |
| GTTTGCGAGGTTGTTGATGG+CGG | 0.604157 | 3_2:-80241713 | Msa0429050:intron |
| AACCTGTTAAGTGCTTCTAC+CGG | 0.606026 | 3_2:-80242126 | Msa0429050:intron |
| TCAAGCGAACCGGCGGCGAG+AGG | 0.609088 | 3_2:-80241871 | Msa0429050:intron |
| ACCTTGCTCAAGCGAACCGG+CGG | 0.627328 | 3_2:-80241878 | Msa0429050:intron |
| AGTCATCATGATCCTCGCAA+TGG | 0.640456 | 3_2:+80241540 | Msa0429070:CDS |
| GCAAAGCGTCACTTCGTACG+TGG | 0.647924 | 3_2:+80241643 | Msa0429070:CDS |
| TCGCTTGAGCAAGGTGAACG+TGG | 0.649304 | 3_2:+80241886 | Msa0429070:CDS |
| CGTTGGGAGAGTGTTATGAA+GGG | 0.659064 | 3_2:+80242034 | Msa0429070:CDS |
| GTGGAATTCCGTGCATGCTG+AGG | 0.659142 | 3_2:-80241573 | Msa0429050:intron |
| AACCTCGCAAACGAAGCCTT+AGG | 0.661102 | 3_2:+80241724 | Msa0429070:CDS |
| GTTAGCGGATTTGACTGAGT+CGG | 0.668658 | 3_2:+80241975 | Msa0429070:CDS |
| AGCTTTGAGAAGATACGAAG+CGG | 0.671113 | 3_2:-80241785 | Msa0429050:intron |
| AAACTCGCAACGAGTGAACG+CGG | 0.681953 | 3_2:+80241937 | Msa0429070:CDS |
| GGAAGATTAAAGCCATTGCG+AGG | 0.704504 | 3_2:-80241552 | Msa0429050:intron |
| TGACTTGTAAACCGATGTCG+CGG | 0.717200 | 3_2:-80241522 | Msa0429050:intron |
| GGCGTCGAGTGATTCAAGAG+CGG | 0.721407 | 3_2:-80241839 | Msa0429050:intron |
| TCTACCGGCACAGAATACTG+TGG | 0.745841 | 3_2:-80242111 | Msa0429050:intron |
| GGCGGCGAGAGGAGACAACG+CGG | 0.761382 | 3_2:-80241860 | Msa0429050:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTATAAGGAAGCTCATGAAT+TGG | + | chr3_2:80242086-80242105 | Msa0429070:CDS | 30.0% | |
| ! | AGTTTAGCGAGGAATTTTTG+TGG | - | chr3_2:80241595-80241614 | Msa0429050:intron | 35.0% |
| !!! | TTGCTTTTCGGTTTTGGAAT+CGG | - | chr3_2:80241618-80241637 | Msa0429050:intron | 35.0% |
| !! | CTTTGATTGCTTTTCGGTTT+TGG | - | chr3_2:80241624-80241643 | Msa0429050:intron | 35.0% |
| !! | GCTTTGCTTTGATTGCTTTT+CGG | - | chr3_2:80241630-80241649 | Msa0429050:intron | 35.0% |
| GTGTTATGAAGGGAAGAAAA+TGG | + | chr3_2:80242044-80242063 | Msa0429070:CDS | 35.0% | |
| GGAAGCAAAGAAAGCTTATA+AGG | + | chr3_2:80242071-80242090 | Msa0429070:CDS | 35.0% | |
| TTAATCTTCCTCAGCATGCA+CGG | + | chr3_2:80241565-80241584 | Msa0429070:CDS | 40.0% | |
| !!! | ATCGAGTTCGATCGCTTTTT+CGG | - | chr3_2:80241755-80241774 | Msa0429050:intron | 40.0% |
| !! | AGCTTTGAGAAGATACGAAG+CGG | - | chr3_2:80241788-80241807 | Msa0429050:intron | 40.0% |
| GCTATGATTCTCGATCTTCA+AGG | + | chr3_2:80241805-80241824 | Msa0429070:CDS | 40.0% | |
| TGAAGGGAAGAAAATGGATG+AGG | + | chr3_2:80242050-80242069 | Msa0429070:CDS | 40.0% | |
| AACCTGTTAAGTGCTTCTAC+CGG | - | chr3_2:80242129-80242148 | Msa0429050:intron | 40.0% | |
| !! | GTAGAAGCACTTAACAGGTT+GGG | + | chr3_2:80242129-80242148 | Msa0429070:CDS | 40.0% |
| ! | TGACTTGTAAACCGATGTCG+CGG | - | chr3_2:80241525-80241544 | Msa0429050:intron | 45.0% |
| AGTCATCATGATCCTCGCAA+TGG | + | chr3_2:80241540-80241559 | Msa0429070:CDS | 45.0% | |
| GGAAGATTAAAGCCATTGCG+AGG | - | chr3_2:80241555-80241574 | Msa0429050:intron | 45.0% | |
| ! | TTTGGAATCGGAGTTTAGCG+AGG | - | chr3_2:80241606-80241625 | Msa0429050:intron | 45.0% |
| !! | GGCGGATCTGGATTTGTTTT+TGG | - | chr3_2:80241698-80241717 | Msa0429050:intron | 45.0% |
| TTCGTTTGCGAGGTTGTTGA+TGG | - | chr3_2:80241719-80241738 | Msa0429050:intron | 45.0% | |
| !! | GTTAGCGGATTTGACTGAGT+CGG | + | chr3_2:80241975-80241994 | Msa0429070:CDS | 45.0% |
| !! | CGTTGGGAGAGTGTTATGAA+GGG | + | chr3_2:80242034-80242053 | Msa0429070:CDS | 45.0% |
| !! | GGTAGAAGCACTTAACAGGT+TGG | + | chr3_2:80242128-80242147 | Msa0429070:CDS | 45.0% |
| ! | GTTTGCGAGGTTGTTGATGG+CGG | - | chr3_2:80241716-80241735 | Msa0429050:intron | 50.0% |
| AACCTCGCAAACGAAGCCTT+AGG | + | chr3_2:80241724-80241743 | Msa0429070:CDS | 50.0% | |
| ! | AGATACGAAGCGGCGTCTTT+TGG | - | chr3_2:80241778-80241797 | Msa0429050:intron | 50.0% |
| TTCACCTTGCTCAAGCGAAC+CGG | - | chr3_2:80241884-80241903 | Msa0429050:intron | 50.0% | |
| AAACTCGCAACGAGTGAACG+CGG | + | chr3_2:80241937-80241956 | Msa0429070:CDS | 50.0% | |
| CACCAAGCTTTCGCGTTCTT+CGG | - | chr3_2:80242012-80242031 | Msa0429050:intron | 50.0% | |
| !! | GCGTTGGGAGAGTGTTATGA+AGG | + | chr3_2:80242033-80242052 | Msa0429070:CDS | 50.0% |
| TCTACCGGCACAGAATACTG+TGG | - | chr3_2:80242114-80242133 | Msa0429050:intron | 50.0% | |
| !! | TGCCGGTAGAAGCACTTAAC+AGG | + | chr3_2:80242124-80242143 | Msa0429070:CDS | 50.0% |
| CAAGCGATGTACCGCGACAT+CGG | + | chr3_2:80241511-80241530 | Msa0429070:CDS | 55.0% | |
| GTGGAATTCCGTGCATGCTG+AGG | - | chr3_2:80241576-80241595 | Msa0429050:intron | 55.0% | |
| ! | GCAAAGCGTCACTTCGTACG+TGG | + | chr3_2:80241643-80241662 | Msa0429070:CDS | 55.0% |
| !! | GAGGTTGTTGATGGCGGATC+TGG | - | chr3_2:80241710-80241729 | Msa0429050:intron | 55.0% |
| !!! | CGCTTTTTCGGCTTCGCCTA+AGG | - | chr3_2:80241743-80241762 | Msa0429050:intron | 55.0% |
| ! | GGCGTCGAGTGATTCAAGAG+CGG | - | chr3_2:80241842-80241861 | Msa0429050:intron | 55.0% |
| TCGCTTGAGCAAGGTGAACG+TGG | + | chr3_2:80241886-80241905 | Msa0429070:CDS | 55.0% | |
| ! | TGAACGCGGACGAGTTGACT+CGG | + | chr3_2:80241951-80241970 | Msa0429070:CDS | 55.0% |
| !! | ACGAGTTGACTCGGCGTTAG+CGG | + | chr3_2:80241960-80241979 | Msa0429070:CDS | 55.0% |
| GTCCGAAGAACGCGAAAGCT+TGG | + | chr3_2:80242007-80242026 | Msa0429070:CDS | 55.0% | |
| !! | GCGAAAGCTTGGTGTGCGTT+GGG | + | chr3_2:80242018-80242037 | Msa0429070:CDS | 55.0% |
| CGCCTAAGGCTTCGTTTGCG+AGG | - | chr3_2:80241729-80241748 | Msa0429050:intron | 60.0% | |
| ACCTTGCTCAAGCGAACCGG+CGG | - | chr3_2:80241881-80241900 | Msa0429050:intron | 60.0% | |
| !! | CGCGAAAGCTTGGTGTGCGT+TGG | + | chr3_2:80242017-80242036 | Msa0429070:CDS | 60.0% |
| GGCGCCACAGTATTCTGTGC+CGG | + | chr3_2:80242107-80242126 | Msa0429070:CDS | 60.0% | |
| GGCGGCGAGAGGAGACAACG+CGG | - | chr3_2:80241863-80241882 | Msa0429050:intron | 70.0% | |
| GCGTTGTCTCCTCTCGCCGC+CGG | + | chr3_2:80241862-80241881 | Msa0429070:CDS | 70.0% | |
| TCAAGCGAACCGGCGGCGAG+AGG | - | chr3_2:80241874-80241893 | Msa0429050:intron | 70.0% | |
| !! | GCCGCCGGTTCGCTTGAGCA+AGG | + | chr3_2:80241877-80241896 | Msa0429070:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 80241493 | 80242158 | 80241493 | ID=Msa0429070;Name=Msa0429070 |
| chr3_2 | mRNA | 80241493 | 80242158 | 80241493 | ID=Msa0429070-mRNA-1;Parent=Msa0429070;Name=Msa0429070-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|221 |
| chr3_2 | exon | 80241493 | 80242158 | 80241493 | ID=Msa0429070-mRNA-1:exon:21888;Parent=Msa0429070-mRNA-1 |
| chr3_2 | CDS | 80241493 | 80242158 | 80241493 | ID=Msa0429070-mRNA-1:cds;Parent=Msa0429070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
