AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705550


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705550 MtrunA17_Chr8g0383721 95.763 118 5 0 1 118 242 359 6.56e-64 197
Msa0705550 MtrunA17_Chr5g0411801 69.298 114 35 0 1 114 275 388 5.10e-50 163
Msa0705550 MtrunA17_Chr5g0411931 65.812 117 40 0 1 117 243 359 7.20e-50 162
Msa0705550 MtrunA17_Chr5g0411821 63.478 115 42 0 1 115 243 357 2.53e-49 161
Msa0705550 MtrunA17_Chr4g0064291 67.544 114 37 0 1 114 243 356 2.76e-49 160
Msa0705550 MtrunA17_Chr5g0411901 65.217 115 40 0 1 115 244 358 8.25e-49 159
Msa0705550 MtrunA17_Chr5g0411811 62.609 115 43 0 1 115 243 357 4.30e-48 157
Msa0705550 MtrunA17_Chr5g0411841 66.087 115 39 0 1 115 243 357 5.56e-48 157
Msa0705550 MtrunA17_Chr5g0411881 63.158 114 42 0 2 115 245 358 6.35e-46 151
Msa0705550 MtrunA17_Chr5g0411911 63.158 114 42 0 1 114 245 358 2.72e-45 150
Msa0705550 MtrunA17_Chr8g0367151 64.957 117 41 0 1 117 243 359 1.32e-42 143
Msa0705550 MtrunA17_Chr5g0411791 64.103 117 42 0 1 117 217 333 2.34e-41 139
Msa0705550 MtrunA17_Chr5g0411871 53.509 114 53 0 1 114 258 371 2.45e-41 140
Msa0705550 MtrunA17_Chr5g0411941 61.404 114 44 0 2 115 244 357 4.98e-41 139
Msa0705550 MtrunA17_Chr1g0157021 62.281 114 43 0 1 114 243 356 2.59e-40 137
Msa0705550 MtrunA17_Chr1g0208031 49.123 114 56 2 2 114 242 354 1.36e-29 108
Msa0705550 MtrunA17_Chr2g0279791 48.649 111 56 1 5 115 296 405 6.45e-28 105
Msa0705550 MtrunA17_Chr5g0411861 61.905 63 24 0 55 117 1 63 1.05e-26 94.0
Msa0705550 MtrunA17_Chr2g0279801 46.491 114 49 2 2 114 110 212 5.39e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705550 AT4G39330.1 81.034 116 22 0 1 116 243 358 1.57e-64 199
Msa0705550 AT2G21730.1 71.053 114 33 0 2 115 240 353 1.19e-55 177
Msa0705550 AT2G21890.1 70.175 114 34 0 2 115 239 352 4.04e-54 173
Msa0705550 AT4G37970.1 61.538 117 45 0 1 117 244 360 3.84e-47 155
Msa0705550 AT4G37980.1 73.451 113 30 0 2 114 240 352 2.84e-46 152
Msa0705550 AT4G37990.1 72.807 114 31 0 2 115 240 353 3.06e-45 150
Msa0705550 AT4G34230.1 51.818 110 53 0 5 114 244 353 7.63e-34 120
Msa0705550 AT4G34230.2 50.909 110 54 0 5 114 244 353 6.22e-33 118
Msa0705550 AT3G19450.1 53.153 111 50 2 5 114 245 354 3.57e-32 116
Msa0705550 AT1G72680.1 45.946 111 59 1 5 115 245 354 1.68e-28 106
Msa0705550 AT4G39330.2 76.271 59 14 0 1 59 243 301 3.20e-25 97.1
Msa0705550 AT4G37970.4 63.462 52 19 0 1 52 244 295 3.66e-15 69.7
Msa0705550 AT4G37980.2 69.091 55 17 0 2 56 240 294 1.34e-11 60.1

Find 34 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTCTTTCCATTAGTTGC+AGG 0.301516 4_4:+78307804 Msa0705550:CDS
GTAGACCAAGCAGTGGCATC+AGG 0.373980 4_4:-78307725 None:intergenic
GATGGTTTACCTGCAACTAA+TGG 0.378875 4_4:-78307813 None:intergenic
ACAAAGCTGATGTGAAATAT+CGG 0.393376 4_4:+78308191 Msa0705550:CDS
ACTGAAGTTGAATGGGAAAC+TGG 0.399835 4_4:+78307746 Msa0705550:CDS
ACTGCAGGACGGAAGCTTAT+CGG 0.411894 4_4:+78308043 Msa0705550:intron
CATTCCCTGATGCCACTGCT+TGG 0.448606 4_4:+78307720 Msa0705550:CDS
GAGAGCTCAAGGGGCTTGCT+AGG 0.495481 4_4:-78307783 None:intergenic
CTTGGTCTACTGAAGTTGAA+TGG 0.496517 4_4:+78307738 Msa0705550:CDS
TTGGTCTACTGAAGTTGAAT+GGG 0.524981 4_4:+78307739 Msa0705550:CDS
AGCTTTGTGAAGCCTTTCCA+TGG 0.525881 4_4:-78308176 None:intergenic
GCATGAAATCAACACGGCCA+TGG 0.534341 4_4:+78308159 Msa0705550:CDS
ATGGAAAGACAGAGAGCTCA+AGG 0.539760 4_4:-78307794 None:intergenic
AAATCAACACGGCCATGGAA+AGG 0.552965 4_4:+78308164 Msa0705550:CDS
CTTATCGGAGGGAGTAACAT+TGG 0.554132 4_4:+78308058 Msa0705550:CDS
AATGGGAAACTGGTCACTGT+AGG 0.556394 4_4:+78307756 Msa0705550:CDS
TGGAGGGATGAAAGAGACTC+AGG 0.563071 4_4:+78308078 Msa0705550:CDS
CTACAAACTTGAGAAGGAGT+TGG 0.582204 4_4:-78308230 None:intergenic
GCAGGCTGCTATGGGAACCA+TGG 0.590820 4_4:+78307668 None:intergenic
TGGCCTCTACAAACTTGAGA+AGG 0.595694 4_4:-78308236 None:intergenic
TGGAAAGACAGAGAGCTCAA+GGG 0.604195 4_4:-78307793 None:intergenic
TCGGAGGGAGTAACATTGGA+GGG 0.606287 4_4:+78308062 Msa0705550:CDS
TAGACCAAGCAGTGGCATCA+GGG 0.607212 4_4:-78307724 None:intergenic
ATCGGAGGGAGTAACATTGG+AGG 0.607417 4_4:+78308061 Msa0705550:CDS
ACTCCTTCTCAAGTTTGTAG+AGG 0.609994 4_4:+78308233 Msa0705550:CDS
GGAAAGACAGAGAGCTCAAG+GGG 0.626737 4_4:-78307792 None:intergenic
ATGGGAAACTGGTCACTGTA+GGG 0.633427 4_4:+78307757 Msa0705550:CDS
TGTGTCTATGATGTAGTCCA+TGG 0.640415 4_4:-78307685 None:intergenic
CAGGAGATGCTCGATTTCTG+CGG 0.641521 4_4:+78308097 Msa0705550:CDS
GCAGGACGGAAGCTTATCGG+AGG 0.666483 4_4:+78308046 Msa0705550:intron
CAGGACGGAAGCTTATCGGA+GGG 0.671442 4_4:+78308047 Msa0705550:intron
ATATCGGTTTGTGATTGATG+TGG 0.686371 4_4:+78308207 Msa0705550:CDS
AACTTCAGTAGACCAAGCAG+TGG 0.689529 4_4:-78307732 None:intergenic
TAAGATGCATGAAATCAACA+CGG 0.753982 4_4:+78308153 Msa0705550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTCAAATTCACATATTA+TGG - chr4_4:78307865-78307884 None:intergenic 20.0%
!! TGAAAATTTGGATAATTCAT+TGG - chr4_4:78308007-78308026 None:intergenic 20.0%
! AATTGACAGAGAATTATTGA+TGG - chr4_4:78307834-78307853 None:intergenic 25.0%
! ACATTAGACATGTGAAAATT+TGG - chr4_4:78308019-78308038 None:intergenic 25.0%
! TAAATGTTTCAGGTCAGATT+TGG - chr4_4:78307919-78307938 None:intergenic 30.0%
TGAGCATGTATAAATGTTTC+AGG - chr4_4:78307929-78307948 None:intergenic 30.0%
TAAGATGCATGAAATCAACA+CGG + chr4_4:78308153-78308172 Msa0705550:CDS 30.0%
ACAAAGCTGATGTGAAATAT+CGG + chr4_4:78308191-78308210 Msa0705550:CDS 30.0%
TTGGTCTACTGAAGTTGAAT+GGG + chr4_4:78307739-78307758 Msa0705550:CDS 35.0%
!! ATATCGGTTTGTGATTGATG+TGG + chr4_4:78308207-78308226 Msa0705550:CDS 35.0%
TGTGTCTATGATGTAGTCCA+TGG - chr4_4:78307688-78307707 None:intergenic 40.0%
CTTGGTCTACTGAAGTTGAA+TGG + chr4_4:78307738-78307757 Msa0705550:CDS 40.0%
ACTGAAGTTGAATGGGAAAC+TGG + chr4_4:78307746-78307765 Msa0705550:CDS 40.0%
TCTGTCTTTCCATTAGTTGC+AGG + chr4_4:78307804-78307823 Msa0705550:CDS 40.0%
GATGGTTTACCTGCAACTAA+TGG - chr4_4:78307816-78307835 None:intergenic 40.0%
GGAAGTAACAGACTCATTCT+TGG - chr4_4:78307898-78307917 None:intergenic 40.0%
ATGTCTAATGTCTTGACTGC+AGG + chr4_4:78308028-78308047 Msa0705550:intron 40.0%
CTACAAACTTGAGAAGGAGT+TGG - chr4_4:78308233-78308252 None:intergenic 40.0%
AACTTCAGTAGACCAAGCAG+TGG - chr4_4:78307735-78307754 None:intergenic 45.0%
AATGGGAAACTGGTCACTGT+AGG + chr4_4:78307756-78307775 Msa0705550:CDS 45.0%
ATGGGAAACTGGTCACTGTA+GGG + chr4_4:78307757-78307776 Msa0705550:CDS 45.0%
TGGAAAGACAGAGAGCTCAA+GGG - chr4_4:78307796-78307815 None:intergenic 45.0%
ATGGAAAGACAGAGAGCTCA+AGG - chr4_4:78307797-78307816 None:intergenic 45.0%
CTAATGTCTTGACTGCAGGA+CGG + chr4_4:78308032-78308051 Msa0705550:intron 45.0%
CTTATCGGAGGGAGTAACAT+TGG + chr4_4:78308058-78308077 Msa0705550:CDS 45.0%
AAATCAACACGGCCATGGAA+AGG + chr4_4:78308164-78308183 Msa0705550:CDS 45.0%
! AGCTTTGTGAAGCCTTTCCA+TGG - chr4_4:78308179-78308198 None:intergenic 45.0%
TAGACCAAGCAGTGGCATCA+GGG - chr4_4:78307727-78307746 None:intergenic 50.0%
GGAAAGACAGAGAGCTCAAG+GGG - chr4_4:78307795-78307814 None:intergenic 50.0%
ACTGCAGGACGGAAGCTTAT+CGG + chr4_4:78308043-78308062 Msa0705550:intron 50.0%
ATCGGAGGGAGTAACATTGG+AGG + chr4_4:78308061-78308080 Msa0705550:CDS 50.0%
TCGGAGGGAGTAACATTGGA+GGG + chr4_4:78308062-78308081 Msa0705550:CDS 50.0%
TGGAGGGATGAAAGAGACTC+AGG + chr4_4:78308078-78308097 Msa0705550:CDS 50.0%
CAGGAGATGCTCGATTTCTG+CGG + chr4_4:78308097-78308116 Msa0705550:CDS 50.0%
GCATGAAATCAACACGGCCA+TGG + chr4_4:78308159-78308178 Msa0705550:CDS 50.0%
CATTCCCTGATGCCACTGCT+TGG + chr4_4:78307720-78307739 Msa0705550:CDS 55.0%
GTAGACCAAGCAGTGGCATC+AGG - chr4_4:78307728-78307747 None:intergenic 55.0%
CAGGACGGAAGCTTATCGGA+GGG + chr4_4:78308047-78308066 Msa0705550:intron 55.0%
! GAGAGCTCAAGGGGCTTGCT+AGG - chr4_4:78307786-78307805 None:intergenic 60.0%
GCAGGACGGAAGCTTATCGG+AGG + chr4_4:78308046-78308065 Msa0705550:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 78307672 78308252 78307672 ID=Msa0705550;Name=Msa0705550
chr4_4 mRNA 78307672 78308252 78307672 ID=Msa0705550-mRNA-1;Parent=Msa0705550;Name=Msa0705550-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|118
chr4_4 exon 78307672 78307825 78307672 ID=Msa0705550-mRNA-1:exon:24967;Parent=Msa0705550-mRNA-1
chr4_4 exon 78308050 78308252 78308050 ID=Msa0705550-mRNA-1:exon:24968;Parent=Msa0705550-mRNA-1
chr4_4 CDS 78307672 78307825 78307672 ID=Msa0705550-mRNA-1:cds;Parent=Msa0705550-mRNA-1
chr4_4 CDS 78308050 78308252 78308050 ID=Msa0705550-mRNA-1:cds;Parent=Msa0705550-mRNA-1
Gene Sequence

>Msa0705550

GCTGCTATGGGAACCATGGACTACATCATAGACACAATATCTGCTGCTCATTCCCTGATGCCACTGCTTGGTCTACTGAAGTTGAATGGGAAACTGGTCACTGTAGGGTTGCCTAGCAAGCCCCTTGAGCTCTCTGTCTTTCCATTAGTTGCAGGACGGAAGCTTATCGGAGGGAGTAACATTGGAGGGATGAAAGAGACTCAGGAGATGCTCGATTTCTGCGGCAAGCACAACATAACTGCAGATATTGAGTTGATTAAGATGCATGAAATCAACACGGCCATGGAAAGGCTTCACAAAGCTGATGTGAAATATCGGTTTGTGATTGATGTGGCCAACTCCTTCTCAAGTTTGTAG

Protein sequence

>Msa0705550

AAMGTMDYIIDTISAAHSLMPLLGLLKLNGKLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHEINTAMERLHKADVKYRFVIDVANSFSSL*