AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1371320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371320 MtrunA17_Chr3g0138201 98.718 78 1 0 1 78 1 78 1.91e-53 163
Msa1371320 MtrunA17_Chr3g0137931 43.284 134 70 3 12 143 22 151 9.63e-32 110
Msa1371320 MtrunA17_Chr6g0477991 43.000 100 53 3 43 142 7 102 5.80e-21 81.6
Msa1371320 MtrunA17_Chr6g0476161 44.737 76 37 4 58 132 6 77 1.08e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371320 AT2G18660.1 28.676 136 83 5 7 140 4 127 7.43e-18 74.7
Msa1371320 AT4G30380.2 33.588 131 70 6 15 140 5 123 1.71e-15 68.6
Msa1371320 AT4G30380.1 32.824 131 69 6 15 140 5 121 3.77e-13 62.4

Find 58 sgRNAs with CRISPR-Local

Find 305 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATAAATTTCTACTTTC+AGG 0.148845 tig0019197:-65692 None:intergenic
TGCTCTGAAACTTATTTAAA+TGG 0.172664 tig0019197:+67796 Msa1371320:three_prime_UTR
TTAGTCAATGTAATTTAAAA+AGG 0.192566 tig0019197:-67906 None:intergenic
GAGCACAATCTTCTGTTTCT+AGG 0.213213 tig0019197:+67305 Msa1371320:CDS
GTTCAGCTGGAGAAGGTATA+TGG 0.236425 tig0019197:+67176 Msa1371320:CDS
TTCAGGTGAAATTATTGATT+TGG 0.251404 tig0019197:-65675 None:intergenic
AAGACAGCGACGGCGTGTTT+TGG 0.285235 tig0019197:+67115 Msa1371320:intron
GAAAGGCTTAAATGCACTTT+TGG 0.291517 tig0019197:+68040 Msa1371320:three_prime_UTR
AACTTAGATGGCGGCATTAT+AGG 0.308157 tig0019197:+67751 Msa1371320:three_prime_UTR
GTTCCTCGGACTTGCATTCC+TGG 0.318288 tig0019197:+67253 Msa1371320:CDS
GATGGCGGCATTATAGGAAT+TGG 0.338158 tig0019197:+67757 Msa1371320:three_prime_UTR
AGGGTTTGAAAATTTGAAAT+AGG 0.368924 tig0019197:+67723 Msa1371320:intron
AGAGGAGAAGGATGATGATT+AGG 0.369225 tig0019197:-66813 None:intergenic
TTAGATGAGTAGTATTTGTA+TGG 0.372297 tig0019197:+67841 Msa1371320:three_prime_UTR
GAAGGATGATGATTAGGTAT+TGG 0.373122 tig0019197:-66807 None:intergenic
AAATAGGCAATGAACTTAGA+TGG 0.389567 tig0019197:+67739 Msa1371320:three_prime_UTR
TAACAGTATTGATAGGCAAC+AGG 0.402579 tig0019197:+66634 Msa1371320:intron
AAGAAAACAGGCTGTCCTCT+TGG 0.405308 tig0019197:-67873 None:intergenic
CTGCGTTGACTTTGGCTAAA+CGG 0.414214 tig0019197:+66685 Msa1371320:five_prime_UTR
AGCAACATGTTTGGTTCAGC+TGG 0.419911 tig0019197:+67163 Msa1371320:CDS
ATCGAAACATACAGTAATAC+AGG 0.436841 tig0019197:-68009 None:intergenic
AGAAAACAGGCTGTCCTCTT+GGG 0.444172 tig0019197:-67872 None:intergenic
TTTCCGTCGAGCAACATGTT+TGG 0.450035 tig0019197:+67154 Msa1371320:CDS
TTGGAATTCGATGTTTATCA+AGG 0.457331 tig0019197:-67398 None:intergenic
TTTGAAGTGACCACATCATC+AGG 0.458585 tig0019197:+65866 Msa1371320:five_prime_UTR
AAATTATTGATTTGGATCGC+TGG 0.466977 tig0019197:-65667 None:intergenic
TTCTGTTTCTAGGCCTTCAT+CGG 0.477067 tig0019197:+67315 Msa1371320:CDS
TGTTTCGATGCTTATTTGAA+AGG 0.482212 tig0019197:+68023 Msa1371320:three_prime_UTR
CTCTATGTAACAGTATTGAT+AGG 0.516275 tig0019197:+66627 Msa1371320:intron
GATGATGATTAGGTATTGGA+AGG 0.531918 tig0019197:-66803 None:intergenic
ACAATGGTGCAGCGTGTGGG+AGG 0.533075 tig0019197:+67200 Msa1371320:CDS
GTATAAGTGCAGCTGTTCCT+CGG 0.534469 tig0019197:+67239 Msa1371320:CDS
TGAACAGTGTTGAGAGGAGA+AGG 0.536418 tig0019197:-66825 None:intergenic
TTCAGCTGGAGAAGGTATAT+GGG 0.539255 tig0019197:+67177 Msa1371320:CDS
ATGTTTGGTTCAGCTGGAGA+AGG 0.553087 tig0019197:+67169 Msa1371320:CDS
AACATTTGCAATGATCTGAA+AGG 0.560268 tig0019197:-67368 None:intergenic
AGTGGATGAACAGTGTTGAG+AGG 0.562506 tig0019197:-66831 None:intergenic
ATGGAAGTATCATCCGATGA+AGG 0.570347 tig0019197:-67328 None:intergenic
CAACGTCAGCTTGAGAGAAG+TGG 0.577205 tig0019197:-66849 None:intergenic
TTCATCGGATGATACTTCCA+TGG 0.589310 tig0019197:+67330 Msa1371320:CDS
CAGGCAATAGCATTAGCTGA+TGG 0.593510 tig0019197:-67990 None:intergenic
TGGGACAATGGTGCAGCGTG+TGG 0.603898 tig0019197:+67196 Msa1371320:CDS
TTAATCTGAATAGTTTGACC+AGG 0.609627 tig0019197:-67271 None:intergenic
ATGGCGATCTCTCGCTGCAG+AGG 0.609698 tig0019197:+67815 Msa1371320:three_prime_UTR
GGGACAATGGTGCAGCGTGT+GGG 0.613332 tig0019197:+67197 Msa1371320:CDS
TGCAATGATCTGAAAGGCAG+TGG 0.623356 tig0019197:-67362 None:intergenic
AACAGTATTGATAGGCAACA+GGG 0.624522 tig0019197:+66635 Msa1371320:intron
CACTTCTCTCAAGCTGACGT+TGG 0.627906 tig0019197:+66850 Msa1371320:CDS
GGCAGTGGTGGAAAGAACCA+TGG 0.636283 tig0019197:-67347 None:intergenic
GAACCAAACATGTTGCTCGA+CGG 0.639586 tig0019197:-67157 None:intergenic
GGAGAAGGTATATGGGACAA+TGG 0.640344 tig0019197:+67184 Msa1371320:CDS
GTATTGATAGGCAACAGGGT+TGG 0.658613 tig0019197:+66639 Msa1371320:intron
AGCGTGTGGGAGGCTGTATG+AGG 0.663788 tig0019197:+67210 Msa1371320:CDS
AATGATCTGAAAGGCAGTGG+TGG 0.664140 tig0019197:-67359 None:intergenic
GTGGGAGGCTGTATGAGGTG+AGG 0.677893 tig0019197:+67215 Msa1371320:CDS
TGACCAGGAATGCAAGTCCG+AGG 0.678065 tig0019197:-67256 None:intergenic
CATGGACGTACCTGATGATG+TGG 0.686912 tig0019197:-65876 None:intergenic
TAGGCAATGAACTTAGATGG+CGG 0.714699 tig0019197:+67742 Msa1371320:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTATAATTTAAAAAA+TGG + tig0019197:66135-66154 Msa1371320:intron 0.0%
!! AATATTTATAATTTAAAAAA+TGG + tig0019197:66135-66154 Msa1371320:intron 0.0%
!!! GCTTTTTTAGATTTTTTTAA+TGG + tig0019197:66348-66367 Msa1371320:intron 15.0%
!! AAAATATACTACTAGTATAT+TGG - tig0019197:66481-66500 None:intergenic 15.0%
!! ATAATAATATAACTTACTGT+TGG - tig0019197:67420-67439 None:intergenic 15.0%
!! TAGTCAATGTAATTTAAAAA+GGG - tig0019197:67908-67927 None:intergenic 15.0%
!! TTAGTCAATGTAATTTAAAA+AGG - tig0019197:67909-67928 None:intergenic 15.0%
!!! GCTTTTTTAGATTTTTTTAA+TGG + tig0019197:66348-66367 Msa1371320:intron 15.0%
!! AAAATATACTACTAGTATAT+TGG - tig0019197:66481-66500 None:intergenic 15.0%
!! ATAATAATATAACTTACTGT+TGG - tig0019197:67420-67439 None:intergenic 15.0%
!! TAGTCAATGTAATTTAAAAA+GGG - tig0019197:67908-67927 None:intergenic 15.0%
!! TTAGTCAATGTAATTTAAAA+AGG - tig0019197:67909-67928 None:intergenic 15.0%
!! TCTAATAAATTTCTACTTTC+AGG - tig0019197:65695-65714 None:intergenic 20.0%
!! AAACAAAACTTAATTTACTC+TGG - tig0019197:65948-65967 None:intergenic 20.0%
!! TGGAAATAATAACAAATGAA+GGG + tig0019197:66248-66267 Msa1371320:intron 20.0%
!! TAATTCCAAAAACAAATTCA+TGG - tig0019197:66316-66335 None:intergenic 20.0%
!!! AGTATATTTTTTTTATCGAG+AGG + tig0019197:66491-66510 Msa1371320:intron 20.0%
!!! GATTCTCTTTTTAAATCTAA+CGG - tig0019197:66980-66999 None:intergenic 20.0%
!! ATATGATATGATGAATCATT+AGG + tig0019197:67442-67461 Msa1371320:intron 20.0%
!! TTGAAAAATCAAATGTTGAA+TGG + tig0019197:67571-67590 Msa1371320:intron 20.0%
!!! ACAACAATATTTTTCTAGTT+AGG + tig0019197:67672-67691 Msa1371320:intron 20.0%
!! AGTCAATGTAATTTAAAAAG+GGG - tig0019197:67907-67926 None:intergenic 20.0%
!! TCTAATAAATTTCTACTTTC+AGG - tig0019197:65695-65714 None:intergenic 20.0%
!! AAACAAAACTTAATTTACTC+TGG - tig0019197:65948-65967 None:intergenic 20.0%
!! TGGAAATAATAACAAATGAA+GGG + tig0019197:66248-66267 Msa1371320:intron 20.0%
!! TAATTCCAAAAACAAATTCA+TGG - tig0019197:66316-66335 None:intergenic 20.0%
!!! AGTATATTTTTTTTATCGAG+AGG + tig0019197:66491-66510 Msa1371320:intron 20.0%
!!! GATTCTCTTTTTAAATCTAA+CGG - tig0019197:66980-66999 None:intergenic 20.0%
!! ATATGATATGATGAATCATT+AGG + tig0019197:67442-67461 Msa1371320:intron 20.0%
!! TTGAAAAATCAAATGTTGAA+TGG + tig0019197:67571-67590 Msa1371320:intron 20.0%
!!! ACAACAATATTTTTCTAGTT+AGG + tig0019197:67672-67691 Msa1371320:intron 20.0%
!! AGTCAATGTAATTTAAAAAG+GGG - tig0019197:67907-67926 None:intergenic 20.0%
!! TTCAGGTGAAATTATTGATT+TGG - tig0019197:65678-65697 None:intergenic 25.0%
! GTGGAAATAATAACAAATGA+AGG + tig0019197:66247-66266 Msa1371320:intron 25.0%
!!! TTGTTTTTGGAATTACACAA+AGG + tig0019197:66321-66340 Msa1371320:intron 25.0%
!!! TGTTTTTGGAATTACACAAA+GGG + tig0019197:66322-66341 Msa1371320:intron 25.0%
!!! ACAAAGTACTCTTTTTTGAA+GGG - tig0019197:66423-66442 None:intergenic 25.0%
!!! AACAAAGTACTCTTTTTTGA+AGG - tig0019197:66424-66443 None:intergenic 25.0%
!!! GAGTTTCTAGAGTATTTTAT+GGG - tig0019197:66576-66595 None:intergenic 25.0%
! AACTAATTGAAGAAACTATG+AGG - tig0019197:66735-66754 None:intergenic 25.0%
!!! AACAAATTTGTTGTGTTTTG+TGG + tig0019197:67084-67103 Msa1371320:intron 25.0%
!!! TTATTGTTTATTTTCCGTGT+TGG + tig0019197:67493-67512 Msa1371320:intron 25.0%
! AAAAATCAAATGTTGAATGG+TGG + tig0019197:67574-67593 Msa1371320:intron 25.0%
!! AGGGTTTGAAAATTTGAAAT+AGG + tig0019197:67723-67742 Msa1371320:intron 25.0%
!!! TGCTCTGAAACTTATTTAAA+TGG + tig0019197:67796-67815 Msa1371320:three_prime_UTR 25.0%
! TTAGATGAGTAGTATTTGTA+TGG + tig0019197:67841-67860 Msa1371320:three_prime_UTR 25.0%
! GTCAATGTAATTTAAAAAGG+GGG - tig0019197:67906-67925 None:intergenic 25.0%
!! TTCAGGTGAAATTATTGATT+TGG - tig0019197:65678-65697 None:intergenic 25.0%
! GTGGAAATAATAACAAATGA+AGG + tig0019197:66247-66266 Msa1371320:intron 25.0%
!!! TTGTTTTTGGAATTACACAA+AGG + tig0019197:66321-66340 Msa1371320:intron 25.0%
!!! TGTTTTTGGAATTACACAAA+GGG + tig0019197:66322-66341 Msa1371320:intron 25.0%
!!! ACAAAGTACTCTTTTTTGAA+GGG - tig0019197:66423-66442 None:intergenic 25.0%
!!! AACAAAGTACTCTTTTTTGA+AGG - tig0019197:66424-66443 None:intergenic 25.0%
!!! GAGTTTCTAGAGTATTTTAT+GGG - tig0019197:66576-66595 None:intergenic 25.0%
! AACTAATTGAAGAAACTATG+AGG - tig0019197:66735-66754 None:intergenic 25.0%
!!! AACAAATTTGTTGTGTTTTG+TGG + tig0019197:67084-67103 Msa1371320:intron 25.0%
!!! TTATTGTTTATTTTCCGTGT+TGG + tig0019197:67493-67512 Msa1371320:intron 25.0%
! AAAAATCAAATGTTGAATGG+TGG + tig0019197:67574-67593 Msa1371320:intron 25.0%
!! AGGGTTTGAAAATTTGAAAT+AGG + tig0019197:67723-67742 Msa1371320:intron 25.0%
!!! TGCTCTGAAACTTATTTAAA+TGG + tig0019197:67796-67815 Msa1371320:three_prime_UTR 25.0%
! TTAGATGAGTAGTATTTGTA+TGG + tig0019197:67841-67860 Msa1371320:three_prime_UTR 25.0%
! GTCAATGTAATTTAAAAAGG+GGG - tig0019197:67906-67925 None:intergenic 25.0%
!! AAATTATTGATTTGGATCGC+TGG - tig0019197:65670-65689 None:intergenic 30.0%
!!! AAAGTACTCTTTTTTGAAGG+GGG - tig0019197:66421-66440 None:intergenic 30.0%
!!! CAAAGTACTCTTTTTTGAAG+GGG - tig0019197:66422-66441 None:intergenic 30.0%
!!! AACAGATTCTTTTTTCTGAG+AGG - tig0019197:66450-66469 None:intergenic 30.0%
!!! CGAGTTTCTAGAGTATTTTA+TGG - tig0019197:66577-66596 None:intergenic 30.0%
! AGAGACGGATCAAATTTTAT+GGG - tig0019197:66611-66630 None:intergenic 30.0%
! TAGAGACGGATCAAATTTTA+TGG - tig0019197:66612-66631 None:intergenic 30.0%
TCAATACTGTTACATAGAGA+CGG - tig0019197:66626-66645 None:intergenic 30.0%
!! CTCTATGTAACAGTATTGAT+AGG + tig0019197:66627-66646 Msa1371320:intron 30.0%
TTTCTCTCTAAACTCAACAT+CGG + tig0019197:67003-67022 Msa1371320:intron 30.0%
TTAATCTGAATAGTTTGACC+AGG - tig0019197:67274-67293 None:intergenic 30.0%
AACATTTGCAATGATCTGAA+AGG - tig0019197:67371-67390 None:intergenic 30.0%
TTGGAATTCGATGTTTATCA+AGG - tig0019197:67401-67420 None:intergenic 30.0%
AATGAAATTGTGAAAACGAG+AGG + tig0019197:67613-67632 Msa1371320:intron 30.0%
!! TCTAGTTAGGTACTGAAATA+TGG + tig0019197:67685-67704 Msa1371320:intron 30.0%
!!! TATGGTATTTTTTCATGTGC+AGG + tig0019197:67703-67722 Msa1371320:intron 30.0%
!!! ATGGTATTTTTTCATGTGCA+GGG + tig0019197:67704-67723 Msa1371320:intron 30.0%
AAATAGGCAATGAACTTAGA+TGG + tig0019197:67739-67758 Msa1371320:three_prime_UTR 30.0%
ATCGAAACATACAGTAATAC+AGG - tig0019197:68012-68031 None:intergenic 30.0%
TGTTTCGATGCTTATTTGAA+AGG + tig0019197:68023-68042 Msa1371320:three_prime_UTR 30.0%
!! AAATTATTGATTTGGATCGC+TGG - tig0019197:65670-65689 None:intergenic 30.0%
!!! AAAGTACTCTTTTTTGAAGG+GGG - tig0019197:66421-66440 None:intergenic 30.0%
!!! CAAAGTACTCTTTTTTGAAG+GGG - tig0019197:66422-66441 None:intergenic 30.0%
!!! AACAGATTCTTTTTTCTGAG+AGG - tig0019197:66450-66469 None:intergenic 30.0%
!!! CGAGTTTCTAGAGTATTTTA+TGG - tig0019197:66577-66596 None:intergenic 30.0%
! AGAGACGGATCAAATTTTAT+GGG - tig0019197:66611-66630 None:intergenic 30.0%
! TAGAGACGGATCAAATTTTA+TGG - tig0019197:66612-66631 None:intergenic 30.0%
TCAATACTGTTACATAGAGA+CGG - tig0019197:66626-66645 None:intergenic 30.0%
!! CTCTATGTAACAGTATTGAT+AGG + tig0019197:66627-66646 Msa1371320:intron 30.0%
TTTCTCTCTAAACTCAACAT+CGG + tig0019197:67003-67022 Msa1371320:intron 30.0%
TTAATCTGAATAGTTTGACC+AGG - tig0019197:67274-67293 None:intergenic 30.0%
AACATTTGCAATGATCTGAA+AGG - tig0019197:67371-67390 None:intergenic 30.0%
TTGGAATTCGATGTTTATCA+AGG - tig0019197:67401-67420 None:intergenic 30.0%
AATGAAATTGTGAAAACGAG+AGG + tig0019197:67613-67632 Msa1371320:intron 30.0%
!! TCTAGTTAGGTACTGAAATA+TGG + tig0019197:67685-67704 Msa1371320:intron 30.0%
!!! TATGGTATTTTTTCATGTGC+AGG + tig0019197:67703-67722 Msa1371320:intron 30.0%
!!! ATGGTATTTTTTCATGTGCA+GGG + tig0019197:67704-67723 Msa1371320:intron 30.0%
AAATAGGCAATGAACTTAGA+TGG + tig0019197:67739-67758 Msa1371320:three_prime_UTR 30.0%
ATCGAAACATACAGTAATAC+AGG - tig0019197:68012-68031 None:intergenic 30.0%
TGTTTCGATGCTTATTTGAA+AGG + tig0019197:68023-68042 Msa1371320:three_prime_UTR 30.0%
!! TACTCTGGAGTTTAATGAGA+TGG - tig0019197:65933-65952 None:intergenic 35.0%
!! GGCATCCATGAATTTGTTTT+TGG + tig0019197:66308-66327 Msa1371320:intron 35.0%
!! TAACAGTATTGATAGGCAAC+AGG + tig0019197:66634-66653 Msa1371320:intron 35.0%
!! AACAGTATTGATAGGCAACA+GGG + tig0019197:66635-66654 Msa1371320:intron 35.0%
!!! AGATTTTTCTGCGTTGACTT+TGG + tig0019197:66677-66696 Msa1371320:five_prime_UTR 35.0%
!! GATGATGATTAGGTATTGGA+AGG - tig0019197:66806-66825 None:intergenic 35.0%
GAAGGATGATGATTAGGTAT+TGG - tig0019197:66810-66829 None:intergenic 35.0%
TATTACTCCACATCACAAGT+TGG + tig0019197:67541-67560 Msa1371320:intron 35.0%
! GAAAGGCTTAAATGCACTTT+TGG + tig0019197:68040-68059 Msa1371320:three_prime_UTR 35.0%
!! TACTCTGGAGTTTAATGAGA+TGG - tig0019197:65933-65952 None:intergenic 35.0%
!! GGCATCCATGAATTTGTTTT+TGG + tig0019197:66308-66327 Msa1371320:intron 35.0%
!! TAACAGTATTGATAGGCAAC+AGG + tig0019197:66634-66653 Msa1371320:intron 35.0%
!! AACAGTATTGATAGGCAACA+GGG + tig0019197:66635-66654 Msa1371320:intron 35.0%
!!! AGATTTTTCTGCGTTGACTT+TGG + tig0019197:66677-66696 Msa1371320:five_prime_UTR 35.0%
!! GATGATGATTAGGTATTGGA+AGG - tig0019197:66806-66825 None:intergenic 35.0%
GAAGGATGATGATTAGGTAT+TGG - tig0019197:66810-66829 None:intergenic 35.0%
TATTACTCCACATCACAAGT+TGG + tig0019197:67541-67560 Msa1371320:intron 35.0%
! TTTGAAGTGACCACATCATC+AGG + tig0019197:65866-65885 Msa1371320:five_prime_UTR 40.0%
AAGAAGAATCATGAAGCGCA+TGG - tig0019197:65897-65916 None:intergenic 40.0%
AACCAGTAACTACCAACCAA+GGG + tig0019197:66163-66182 Msa1371320:intron 40.0%
AAATTATGGGTCTCTGATGC+CGG - tig0019197:66195-66214 None:intergenic 40.0%
ACAGGCAAGGTCCAAATTAT+GGG - tig0019197:66208-66227 None:intergenic 40.0%
GGCAGTGGGATCAAAATTAT+GGG - tig0019197:66275-66294 None:intergenic 40.0%
TGGCAGTGGGATCAAAATTA+TGG - tig0019197:66276-66295 None:intergenic 40.0%
ACAAGTGCTCATTCGTATGA+GGG - tig0019197:66394-66413 None:intergenic 40.0%
AACAAGTGCTCATTCGTATG+AGG - tig0019197:66395-66414 None:intergenic 40.0%
AGTCAACGCAGAAAAATCTG+TGG - tig0019197:66676-66695 None:intergenic 40.0%
AGAGGAGAAGGATGATGATT+AGG - tig0019197:66816-66835 None:intergenic 40.0%
TAAAAAGGGAGGGCTGTAAT+GGG - tig0019197:66884-66903 None:intergenic 40.0%
! ACAACTGATAATGCGCCAAA+CGG - tig0019197:66952-66971 None:intergenic 40.0%
! TTCAGCTGGAGAAGGTATAT+GGG + tig0019197:67177-67196 Msa1371320:CDS 40.0%
! GAGCACAATCTTCTGTTTCT+AGG + tig0019197:67305-67324 Msa1371320:CDS 40.0%
!! TTCTGTTTCTAGGCCTTCAT+CGG + tig0019197:67315-67334 Msa1371320:CDS 40.0%
!! ATGGAAGTATCATCCGATGA+AGG - tig0019197:67331-67350 None:intergenic 40.0%
TTCATCGGATGATACTTCCA+TGG + tig0019197:67330-67349 Msa1371320:CDS 40.0%
!!! TTTCCGTGTTGGTTGTGTTT+TGG + tig0019197:67504-67523 Msa1371320:intron 40.0%
TCACCAAAACACAACCAACA+CGG - tig0019197:67510-67529 None:intergenic 40.0%
CAACTAACCAACTTGTGATG+TGG - tig0019197:67551-67570 None:intergenic 40.0%
TAGGCAATGAACTTAGATGG+CGG + tig0019197:67742-67761 Msa1371320:three_prime_UTR 40.0%
! AACTTAGATGGCGGCATTAT+AGG + tig0019197:67751-67770 Msa1371320:three_prime_UTR 40.0%
! GTATGGATTTTGTTCCCAAG+AGG + tig0019197:67858-67877 Msa1371320:three_prime_UTR 40.0%
GGGGGTAATTTGAAGAAAAC+AGG - tig0019197:67888-67907 None:intergenic 40.0%
! TTTGAAGTGACCACATCATC+AGG + tig0019197:65866-65885 Msa1371320:five_prime_UTR 40.0%
AAGAAGAATCATGAAGCGCA+TGG - tig0019197:65897-65916 None:intergenic 40.0%
AACCAGTAACTACCAACCAA+GGG + tig0019197:66163-66182 Msa1371320:intron 40.0%
AAATTATGGGTCTCTGATGC+CGG - tig0019197:66195-66214 None:intergenic 40.0%
ACAGGCAAGGTCCAAATTAT+GGG - tig0019197:66208-66227 None:intergenic 40.0%
GGCAGTGGGATCAAAATTAT+GGG - tig0019197:66275-66294 None:intergenic 40.0%
TGGCAGTGGGATCAAAATTA+TGG - tig0019197:66276-66295 None:intergenic 40.0%
ACAAGTGCTCATTCGTATGA+GGG - tig0019197:66394-66413 None:intergenic 40.0%
AACAAGTGCTCATTCGTATG+AGG - tig0019197:66395-66414 None:intergenic 40.0%
AGTCAACGCAGAAAAATCTG+TGG - tig0019197:66676-66695 None:intergenic 40.0%
AGAGGAGAAGGATGATGATT+AGG - tig0019197:66816-66835 None:intergenic 40.0%
TAAAAAGGGAGGGCTGTAAT+GGG - tig0019197:66884-66903 None:intergenic 40.0%
! ACAACTGATAATGCGCCAAA+CGG - tig0019197:66952-66971 None:intergenic 40.0%
! TTCAGCTGGAGAAGGTATAT+GGG + tig0019197:67177-67196 Msa1371320:CDS 40.0%
! GAGCACAATCTTCTGTTTCT+AGG + tig0019197:67305-67324 Msa1371320:CDS 40.0%
!! TTCTGTTTCTAGGCCTTCAT+CGG + tig0019197:67315-67334 Msa1371320:CDS 40.0%
!! ATGGAAGTATCATCCGATGA+AGG - tig0019197:67331-67350 None:intergenic 40.0%
TTCATCGGATGATACTTCCA+TGG + tig0019197:67330-67349 Msa1371320:CDS 40.0%
!!! TTTCCGTGTTGGTTGTGTTT+TGG + tig0019197:67504-67523 Msa1371320:intron 40.0%
TCACCAAAACACAACCAACA+CGG - tig0019197:67510-67529 None:intergenic 40.0%
CAACTAACCAACTTGTGATG+TGG - tig0019197:67551-67570 None:intergenic 40.0%
TAGGCAATGAACTTAGATGG+CGG + tig0019197:67742-67761 Msa1371320:three_prime_UTR 40.0%
! AACTTAGATGGCGGCATTAT+AGG + tig0019197:67751-67770 Msa1371320:three_prime_UTR 40.0%
! GTATGGATTTTGTTCCCAAG+AGG + tig0019197:67858-67877 Msa1371320:three_prime_UTR 40.0%
GGGGGTAATTTGAAGAAAAC+AGG - tig0019197:67888-67907 None:intergenic 40.0%
!! CTTGGTTGGTAGTTACTGGT+TGG - tig0019197:66164-66183 None:intergenic 45.0%
CAACCAGTAACTACCAACCA+AGG + tig0019197:66162-66181 Msa1371320:intron 45.0%
GGCATCAGAGACCCATAATT+TGG + tig0019197:66194-66213 Msa1371320:intron 45.0%
GACAGGCAAGGTCCAAATTA+TGG - tig0019197:66209-66228 None:intergenic 45.0%
CTTACGAGTTACGAACAGAC+AGG - tig0019197:66226-66245 None:intergenic 45.0%
CTGTTCGTAACTCGTAAGTG+TGG + tig0019197:66228-66247 Msa1371320:intron 45.0%
AAATTCATGGATGCCCTCGT+CGG - tig0019197:66303-66322 None:intergenic 45.0%
! GTATTGATAGGCAACAGGGT+TGG + tig0019197:66639-66658 Msa1371320:intron 45.0%
!! CTGCGTTGACTTTGGCTAAA+CGG + tig0019197:66685-66704 Msa1371320:five_prime_UTR 45.0%
! TGAACAGTGTTGAGAGGAGA+AGG - tig0019197:66828-66847 None:intergenic 45.0%
! AGTGGATGAACAGTGTTGAG+AGG - tig0019197:66834-66853 None:intergenic 45.0%
GTAAAAAGGGAGGGCTGTAA+TGG - tig0019197:66885-66904 None:intergenic 45.0%
GGCACTTACGTAAAAAGGGA+GGG - tig0019197:66894-66913 None:intergenic 45.0%
AGAGGATGTGGCTATTGTTG+AGG - tig0019197:66928-66947 None:intergenic 45.0%
TTTCCGTCGAGCAACATGTT+TGG + tig0019197:67154-67173 Msa1371320:CDS 45.0%
GAACCAAACATGTTGCTCGA+CGG - tig0019197:67160-67179 None:intergenic 45.0%
! AGCAACATGTTTGGTTCAGC+TGG + tig0019197:67163-67182 Msa1371320:CDS 45.0%
! ATGTTTGGTTCAGCTGGAGA+AGG + tig0019197:67169-67188 Msa1371320:CDS 45.0%
! GTTCAGCTGGAGAAGGTATA+TGG + tig0019197:67176-67195 Msa1371320:CDS 45.0%
! GGAGAAGGTATATGGGACAA+TGG + tig0019197:67184-67203 Msa1371320:CDS 45.0%
GTATAAGTGCAGCTGTTCCT+CGG + tig0019197:67239-67258 Msa1371320:CDS 45.0%
AATGATCTGAAAGGCAGTGG+TGG - tig0019197:67362-67381 None:intergenic 45.0%
TGCAATGATCTGAAAGGCAG+TGG - tig0019197:67365-67384 None:intergenic 45.0%
! GATGGCGGCATTATAGGAAT+TGG + tig0019197:67757-67776 Msa1371320:three_prime_UTR 45.0%
AGAAAACAGGCTGTCCTCTT+GGG - tig0019197:67875-67894 None:intergenic 45.0%
AAGAAAACAGGCTGTCCTCT+TGG - tig0019197:67876-67895 None:intergenic 45.0%
CAGGCAATAGCATTAGCTGA+TGG - tig0019197:67993-68012 None:intergenic 45.0%
!! CTTGGTTGGTAGTTACTGGT+TGG - tig0019197:66164-66183 None:intergenic 45.0%
CAACCAGTAACTACCAACCA+AGG + tig0019197:66162-66181 Msa1371320:intron 45.0%
GGCATCAGAGACCCATAATT+TGG + tig0019197:66194-66213 Msa1371320:intron 45.0%
GACAGGCAAGGTCCAAATTA+TGG - tig0019197:66209-66228 None:intergenic 45.0%
CTTACGAGTTACGAACAGAC+AGG - tig0019197:66226-66245 None:intergenic 45.0%
CTGTTCGTAACTCGTAAGTG+TGG + tig0019197:66228-66247 Msa1371320:intron 45.0%
AAATTCATGGATGCCCTCGT+CGG - tig0019197:66303-66322 None:intergenic 45.0%
! GTATTGATAGGCAACAGGGT+TGG + tig0019197:66639-66658 Msa1371320:intron 45.0%
!! CTGCGTTGACTTTGGCTAAA+CGG + tig0019197:66685-66704 Msa1371320:five_prime_UTR 45.0%
! TGAACAGTGTTGAGAGGAGA+AGG - tig0019197:66828-66847 None:intergenic 45.0%
! AGTGGATGAACAGTGTTGAG+AGG - tig0019197:66834-66853 None:intergenic 45.0%
GTAAAAAGGGAGGGCTGTAA+TGG - tig0019197:66885-66904 None:intergenic 45.0%
GGCACTTACGTAAAAAGGGA+GGG - tig0019197:66894-66913 None:intergenic 45.0%
AGAGGATGTGGCTATTGTTG+AGG - tig0019197:66928-66947 None:intergenic 45.0%
TTTCCGTCGAGCAACATGTT+TGG + tig0019197:67154-67173 Msa1371320:CDS 45.0%
GAACCAAACATGTTGCTCGA+CGG - tig0019197:67160-67179 None:intergenic 45.0%
! AGCAACATGTTTGGTTCAGC+TGG + tig0019197:67163-67182 Msa1371320:CDS 45.0%
! ATGTTTGGTTCAGCTGGAGA+AGG + tig0019197:67169-67188 Msa1371320:CDS 45.0%
! GTTCAGCTGGAGAAGGTATA+TGG + tig0019197:67176-67195 Msa1371320:CDS 45.0%
! GGAGAAGGTATATGGGACAA+TGG + tig0019197:67184-67203 Msa1371320:CDS 45.0%
GTATAAGTGCAGCTGTTCCT+CGG + tig0019197:67239-67258 Msa1371320:CDS 45.0%
AATGATCTGAAAGGCAGTGG+TGG - tig0019197:67362-67381 None:intergenic 45.0%
TGCAATGATCTGAAAGGCAG+TGG - tig0019197:67365-67384 None:intergenic 45.0%
! GATGGCGGCATTATAGGAAT+TGG + tig0019197:67757-67776 Msa1371320:three_prime_UTR 45.0%
AGAAAACAGGCTGTCCTCTT+GGG - tig0019197:67875-67894 None:intergenic 45.0%
AAGAAAACAGGCTGTCCTCT+TGG - tig0019197:67876-67895 None:intergenic 45.0%
CAGGCAATAGCATTAGCTGA+TGG - tig0019197:67993-68012 None:intergenic 45.0%
! CATGGACGTACCTGATGATG+TGG - tig0019197:65879-65898 None:intergenic 50.0%
CAGTAACTACCAACCAAGGG+CGG + tig0019197:66166-66185 Msa1371320:intron 50.0%
GAGTTACGAACAGACAGGCA+AGG - tig0019197:66221-66240 None:intergenic 50.0%
CAACGTCAGCTTGAGAGAAG+TGG - tig0019197:66852-66871 None:intergenic 50.0%
CACTTCTCTCAAGCTGACGT+TGG + tig0019197:66850-66869 Msa1371320:CDS 50.0%
GGGCACTTACGTAAAAAGGG+AGG - tig0019197:66895-66914 None:intergenic 50.0%
!! GGGGGGCACTTACGTAAAAA+GGG - tig0019197:66898-66917 None:intergenic 50.0%
!! AGGGGGGCACTTACGTAAAA+AGG - tig0019197:66899-66918 None:intergenic 50.0%
TATTGTTGAGGCAGAGGAGG+GGG - tig0019197:66916-66935 None:intergenic 50.0%
CTATTGTTGAGGCAGAGGAG+GGG - tig0019197:66917-66936 None:intergenic 50.0%
GCTATTGTTGAGGCAGAGGA+GGG - tig0019197:66918-66937 None:intergenic 50.0%
TGTGGCTATTGTTGAGGCAG+AGG - tig0019197:66922-66941 None:intergenic 50.0%
! CATGGACGTACCTGATGATG+TGG - tig0019197:65879-65898 None:intergenic 50.0%
CAGTAACTACCAACCAAGGG+CGG + tig0019197:66166-66185 Msa1371320:intron 50.0%
GAGTTACGAACAGACAGGCA+AGG - tig0019197:66221-66240 None:intergenic 50.0%
CAACGTCAGCTTGAGAGAAG+TGG - tig0019197:66852-66871 None:intergenic 50.0%
CACTTCTCTCAAGCTGACGT+TGG + tig0019197:66850-66869 Msa1371320:CDS 50.0%
GGGCACTTACGTAAAAAGGG+AGG - tig0019197:66895-66914 None:intergenic 50.0%
!! GGGGGGCACTTACGTAAAAA+GGG - tig0019197:66898-66917 None:intergenic 50.0%
!! AGGGGGGCACTTACGTAAAA+AGG - tig0019197:66899-66918 None:intergenic 50.0%
TATTGTTGAGGCAGAGGAGG+GGG - tig0019197:66916-66935 None:intergenic 50.0%
CTATTGTTGAGGCAGAGGAG+GGG - tig0019197:66917-66936 None:intergenic 50.0%
GCTATTGTTGAGGCAGAGGA+GGG - tig0019197:66918-66937 None:intergenic 50.0%
TGTGGCTATTGTTGAGGCAG+AGG - tig0019197:66922-66941 None:intergenic 50.0%
!! CGCCCTTGGTTGGTAGTTAC+TGG - tig0019197:66168-66187 None:intergenic 55.0%
TAACTACCAACCAAGGGCGG+TGG + tig0019197:66169-66188 Msa1371320:intron 55.0%
ATTGTTGAGGCAGAGGAGGG+GGG - tig0019197:66915-66934 None:intergenic 55.0%
GGCTATTGTTGAGGCAGAGG+AGG - tig0019197:66919-66938 None:intergenic 55.0%
TAGCCACATCCTCTGCCGTT+TGG + tig0019197:66934-66953 Msa1371320:intron 55.0%
GATAATGCGCCAAACGGCAG+AGG - tig0019197:66946-66965 None:intergenic 55.0%
GGAACGTGACAAGACAGCGA+CGG + tig0019197:67105-67124 Msa1371320:intron 55.0%
! AAGACAGCGACGGCGTGTTT+TGG + tig0019197:67115-67134 Msa1371320:intron 55.0%
! AGACAGCGACGGCGTGTTTT+GGG + tig0019197:67116-67135 Msa1371320:intron 55.0%
GTTCCTCGGACTTGCATTCC+TGG + tig0019197:67253-67272 Msa1371320:CDS 55.0%
TGACCAGGAATGCAAGTCCG+AGG - tig0019197:67259-67278 None:intergenic 55.0%
! GGCAGTGGTGGAAAGAACCA+TGG - tig0019197:67350-67369 None:intergenic 55.0%
!! CGCCCTTGGTTGGTAGTTAC+TGG - tig0019197:66168-66187 None:intergenic 55.0%
TAACTACCAACCAAGGGCGG+TGG + tig0019197:66169-66188 Msa1371320:intron 55.0%
ATTGTTGAGGCAGAGGAGGG+GGG - tig0019197:66915-66934 None:intergenic 55.0%
GGCTATTGTTGAGGCAGAGG+AGG - tig0019197:66919-66938 None:intergenic 55.0%
TAGCCACATCCTCTGCCGTT+TGG + tig0019197:66934-66953 Msa1371320:intron 55.0%
GATAATGCGCCAAACGGCAG+AGG - tig0019197:66946-66965 None:intergenic 55.0%
GGAACGTGACAAGACAGCGA+CGG + tig0019197:67105-67124 Msa1371320:intron 55.0%
! AAGACAGCGACGGCGTGTTT+TGG + tig0019197:67115-67134 Msa1371320:intron 55.0%
! AGACAGCGACGGCGTGTTTT+GGG + tig0019197:67116-67135 Msa1371320:intron 55.0%
GTTCCTCGGACTTGCATTCC+TGG + tig0019197:67253-67272 Msa1371320:CDS 55.0%
TGACCAGGAATGCAAGTCCG+AGG - tig0019197:67259-67278 None:intergenic 55.0%
! GGCAGTGGTGGAAAGAACCA+TGG - tig0019197:67350-67369 None:intergenic 55.0%
!! CCTCGTCGGTTTGTGGCAGT+GGG - tig0019197:66289-66308 None:intergenic 60.0%
CCACTGCCACAAACCGACGA+GGG + tig0019197:66287-66306 Msa1371320:intron 60.0%
!! TGGATGCCCTCGTCGGTTTG+TGG - tig0019197:66296-66315 None:intergenic 60.0%
! GACAGCGACGGCGTGTTTTG+GGG + tig0019197:67117-67136 Msa1371320:intron 60.0%
!! ACAGCGACGGCGTGTTTTGG+GGG + tig0019197:67118-67137 Msa1371320:intron 60.0%
TGGGACAATGGTGCAGCGTG+TGG + tig0019197:67196-67215 Msa1371320:CDS 60.0%
GGGACAATGGTGCAGCGTGT+GGG + tig0019197:67197-67216 Msa1371320:CDS 60.0%
ACAATGGTGCAGCGTGTGGG+AGG + tig0019197:67200-67219 Msa1371320:CDS 60.0%
AGCGTGTGGGAGGCTGTATG+AGG + tig0019197:67210-67229 Msa1371320:CDS 60.0%
GTGGGAGGCTGTATGAGGTG+AGG + tig0019197:67215-67234 Msa1371320:CDS 60.0%
ATGGCGATCTCTCGCTGCAG+AGG + tig0019197:67815-67834 Msa1371320:three_prime_UTR 60.0%
!! CCTCGTCGGTTTGTGGCAGT+GGG - tig0019197:66289-66308 None:intergenic 60.0%
CCACTGCCACAAACCGACGA+GGG + tig0019197:66287-66306 Msa1371320:intron 60.0%
!! TGGATGCCCTCGTCGGTTTG+TGG - tig0019197:66296-66315 None:intergenic 60.0%
! GACAGCGACGGCGTGTTTTG+GGG + tig0019197:67117-67136 Msa1371320:intron 60.0%
!! ACAGCGACGGCGTGTTTTGG+GGG + tig0019197:67118-67137 Msa1371320:intron 60.0%
TGGGACAATGGTGCAGCGTG+TGG + tig0019197:67196-67215 Msa1371320:CDS 60.0%
GGGACAATGGTGCAGCGTGT+GGG + tig0019197:67197-67216 Msa1371320:CDS 60.0%
ACAATGGTGCAGCGTGTGGG+AGG + tig0019197:67200-67219 Msa1371320:CDS 60.0%
AGCGTGTGGGAGGCTGTATG+AGG + tig0019197:67210-67229 Msa1371320:CDS 60.0%
GTGGGAGGCTGTATGAGGTG+AGG + tig0019197:67215-67234 Msa1371320:CDS 60.0%
ATGGCGATCTCTCGCTGCAG+AGG + tig0019197:67815-67834 Msa1371320:three_prime_UTR 60.0%
TACCAACCAAGGGCGGTGGC+CGG + tig0019197:66173-66192 Msa1371320:intron 65.0%
CCCACTGCCACAAACCGACG+AGG + tig0019197:66286-66305 Msa1371320:intron 65.0%
!! CCCTCGTCGGTTTGTGGCAG+TGG - tig0019197:66290-66309 None:intergenic 65.0%
GCGCCAAACGGCAGAGGATG+TGG - tig0019197:66940-66959 None:intergenic 65.0%
TACCAACCAAGGGCGGTGGC+CGG + tig0019197:66173-66192 Msa1371320:intron 65.0%
CCCACTGCCACAAACCGACG+AGG + tig0019197:66286-66305 Msa1371320:intron 65.0%
!! CCCTCGTCGGTTTGTGGCAG+TGG - tig0019197:66290-66309 None:intergenic 65.0%
GCGCCAAACGGCAGAGGATG+TGG - tig0019197:66940-66959 None:intergenic 65.0%
TGCCGGCCACCGCCCTTGGT+TGG - tig0019197:66178-66197 None:intergenic 75.0%
CTGATGCCGGCCACCGCCCT+TGG - tig0019197:66182-66201 None:intergenic 75.0%
TGCCGGCCACCGCCCTTGGT+TGG - tig0019197:66178-66197 None:intergenic 75.0%
CTGATGCCGGCCACCGCCCT+TGG - tig0019197:66182-66201 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0019197 gene 65666 68062 65666 ID=Msa1371320;Name=Msa1371320
tig0019197 mRNA 65666 68062 65666 ID=Msa1371320-mRNA-1;Parent=Msa1371320;Name=Msa1371320-mRNA-1;_AED=0.17;_eAED=0.17;_QI=182|1|1|1|0|0|6|331|143
tig0019197 exon 65666 65737 65666 ID=Msa1371320-mRNA-1:exon:2226;Parent=Msa1371320-mRNA-1
tig0019197 exon 65850 65887 65850 ID=Msa1371320-mRNA-1:exon:2227;Parent=Msa1371320-mRNA-1
tig0019197 exon 66640 66706 66640 ID=Msa1371320-mRNA-1:exon:2228;Parent=Msa1371320-mRNA-1
tig0019197 exon 66776 66904 66776 ID=Msa1371320-mRNA-1:exon:2229;Parent=Msa1371320-mRNA-1
tig0019197 exon 67122 67422 67122 ID=Msa1371320-mRNA-1:exon:2230;Parent=Msa1371320-mRNA-1
tig0019197 exon 67725 68062 67725 ID=Msa1371320-mRNA-1:exon:2231;Parent=Msa1371320-mRNA-1
tig0019197 five_prime_UTR 65666 65737 65666 ID=Msa1371320-mRNA-1:five_prime_utr;Parent=Msa1371320-mRNA-1
tig0019197 five_prime_UTR 65850 65887 65850 ID=Msa1371320-mRNA-1:five_prime_utr;Parent=Msa1371320-mRNA-1
tig0019197 five_prime_UTR 66640 66706 66640 ID=Msa1371320-mRNA-1:five_prime_utr;Parent=Msa1371320-mRNA-1
tig0019197 five_prime_UTR 66776 66780 66776 ID=Msa1371320-mRNA-1:five_prime_utr;Parent=Msa1371320-mRNA-1
tig0019197 CDS 66781 66904 66781 ID=Msa1371320-mRNA-1:cds;Parent=Msa1371320-mRNA-1
tig0019197 CDS 67122 67422 67122 ID=Msa1371320-mRNA-1:cds;Parent=Msa1371320-mRNA-1
tig0019197 CDS 67725 67731 67725 ID=Msa1371320-mRNA-1:cds;Parent=Msa1371320-mRNA-1
tig0019197 three_prime_UTR 67732 68062 67732 ID=Msa1371320-mRNA-1:three_prime_utr;Parent=Msa1371320-mRNA-1
Gene Sequence

>Msa1371320

ATGTTCAAGTCTCAATATCTTTCCTTCCAATACCTAATCATCATCCTTCTCCTCTCAACACTGTTCATCCACTTCTCTCAAGCTGACGTTGGCACTGCTTCCCATTACAGCCCTCCCTTTTTACCGACGGCGTGTTTTGGGGGAGATGCGTCACAATTTCCGTCGAGCAACATGTTTGGTTCAGCTGGAGAAGGTATATGGGACAATGGTGCAGCGTGTGGGAGGCTGTATGAGGTGAGGTGTATAAGTGCAGCTGTTCCTCGGACTTGCATTCCTGGTCAAACTATTCAGATTAAGATTGTTGATAGAGCACAATCTTCTGTTTCTAGGCCTTCATCGGATGATACTTCCATGGTTCTTTCCACCACTGCCTTTCAGATCATTGCAAATGTTTCTTCATCCTTGATAAACATCGAATTCCAACAGGTTTGA
Protein sequence

>Msa1371320

MFKSQYLSFQYLIIILLLSTLFIHFSQADVGTASHYSPPFLPTACFGGDASQFPSSNMFGSAGEGIWDNGAACGRLYEVRCISAAVPRTCIPGQTIQIKIVDRAQSSVSRPSSDDTSMVLSTTAFQIIANVSSSLINIEFQQV*