AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0462840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0462840 MtrunA17_Chr3g0112791 64.977 217 18 3 1 159 1 217 3.80e-84 246
Msa0462840 MtrunA17_Chr5g0436201 68.493 73 21 1 85 155 135 207 3.10e-31 111
Msa0462840 MtrunA17_Chr7g0240961 59.459 74 26 3 85 155 158 230 2.14e-17 76.3
Msa0462840 MtrunA17_Chr3g0112811 62.687 67 16 4 10 75 1 59 1.65e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0462840 AT5G05830.1 54.118 85 35 3 74 155 116 199 2.59e-18 78.2

Find 52 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTATTGTCTTCTCATT+TGG 0.163355 3_3:-62095531 Msa0462840:three_prime_UTR
CTGCTAGATCCTGAAGTTAT+AGG 0.214776 3_3:+62095828 None:intergenic
CCAGCTTCCAAACATAATTT+AGG 0.216231 3_3:-62095639 Msa0462840:three_prime_UTR
TTCTTATTGTCTTCTCATTT+GGG 0.244244 3_3:-62095530 Msa0462840:three_prime_UTR
GTCTGAATGTTGTGTGGATT+TGG 0.311840 3_3:-62098248 Msa0462840:CDS
AATCTTCATGTCATGGCTTT+GGG 0.337486 3_3:+62098426 None:intergenic
GAATCTTCATGTCATGGCTT+TGG 0.342745 3_3:+62098425 None:intergenic
GAATTTCCTCCTTGCCTGTA+TGG 0.353899 3_3:-62095904 Msa0462840:CDS
CTTCTCATTTGGGTTATGAA+TGG 0.367231 3_3:-62095520 Msa0462840:three_prime_UTR
GGCTTTGAGTTTCTGCAGGT+GGG 0.375964 3_3:+62095943 None:intergenic
TCCTAAAATCCTATAACTTC+AGG 0.376700 3_3:-62095837 Msa0462840:exon
GATACTTCCTAAATTATGTT+TGG 0.383113 3_3:+62095632 None:intergenic
TCATTGCCTTTGTCATCTCC+TGG 0.395813 3_3:-62095881 Msa0462840:CDS
TCCATTGCTAGAAATGTATC+TGG 0.396063 3_3:-62096041 Msa0462840:CDS
CTTTGAGTTTCTGCAGGTGG+GGG 0.420385 3_3:+62095945 None:intergenic
CCTAAATTATGTTTGGAAGC+TGG 0.430685 3_3:+62095639 None:intergenic
CATGGTACCTGTGTTTATAA+TGG 0.435036 3_3:+62095770 None:intergenic
GTAAGATGATTGAGGATTTG+TGG 0.435692 3_3:-62095805 Msa0462840:three_prime_UTR
AAGTTATAACTTATAATAAC+AGG 0.436688 3_3:+62095573 None:intergenic
GCTTTGAGTTTCTGCAGGTG+GGG 0.477438 3_3:+62095944 None:intergenic
AGTAGGCTTTGAGTTTCTGC+AGG 0.480746 3_3:+62095939 None:intergenic
AGGCTTTGAGTTTCTGCAGG+TGG 0.480977 3_3:+62095942 None:intergenic
ATGTTGTGTGGATTTGGATC+TGG 0.492466 3_3:-62098242 Msa0462840:CDS
CAAGGAGGAAATTCAAGAAC+CGG 0.500384 3_3:+62095913 None:intergenic
AACTCTACAGCGGAGAATGT+TGG 0.523212 3_3:-62098307 Msa0462840:CDS
ACCAGATACATTTCTAGCAA+TGG 0.523936 3_3:+62096040 None:intergenic
AATTCAAGAACCGGTGGAGT+AGG 0.535576 3_3:+62095922 None:intergenic
TGTTCAAGTAAGTAACAAGA+AGG 0.544984 3_3:-62095705 Msa0462840:three_prime_UTR
AGTTGAGAATCTTCATGTCA+TGG 0.547212 3_3:+62098419 None:intergenic
CTATAACTTCAGGATCTAGC+AGG 0.548028 3_3:-62095827 Msa0462840:three_prime_UTR
GACAAAGGCAATGACCATAC+AGG 0.555573 3_3:+62095890 None:intergenic
GTCTGTGTCTGAATGTTGTG+TGG 0.557366 3_3:-62098254 Msa0462840:CDS
TCTAGCAGGTAAGATGATTG+AGG 0.586586 3_3:-62095813 Msa0462840:three_prime_UTR
TGCATTGTGTTGAGGAAATG+AGG 0.592329 3_3:-62095465 Msa0462840:three_prime_UTR
ATTGAGACTGCATTGTGTTG+AGG 0.593238 3_3:-62095473 Msa0462840:three_prime_UTR
ACTGTGGTTAATGTTGATGA+TGG 0.601911 3_3:-62098217 Msa0462840:intron
TACAGAACTTGTGAAATCTG+TGG 0.602282 3_3:-62096065 Msa0462840:intron
AATAAGAAGCAGTTGAGACA+AGG 0.603597 3_3:+62095545 None:intergenic
AAACTCAAAGCCTACTCCAC+CGG 0.607970 3_3:-62095932 Msa0462840:CDS
GTTCAAGTAAGTAACAAGAA+GGG 0.613575 3_3:-62095704 Msa0462840:three_prime_UTR
ACTTGTCTTGTCCTGCGTGA+AGG 0.618259 3_3:+62098377 None:intergenic
ACATTGAAAATGCATCTCCT+CGG 0.624698 3_3:-62098330 Msa0462840:CDS
GGATTTGGATCTGGAGACTG+TGG 0.648946 3_3:-62098233 Msa0462840:CDS
GGAGGAAATTCAAGAACCGG+TGG 0.655498 3_3:+62095916 None:intergenic
TCTGTGACCATTATAAACAC+AGG 0.659282 3_3:-62095777 Msa0462840:three_prime_UTR
AGGCAATGACCATACAGGCA+AGG 0.660733 3_3:+62095895 None:intergenic
ATCGAAAATGTCCTTCACGC+AGG 0.666258 3_3:-62098388 Msa0462840:CDS
TTCTCCGCTGTAGAGTTCCG+AGG 0.672525 3_3:+62098313 None:intergenic
CAATGACCATACAGGCAAGG+AGG 0.693990 3_3:+62095898 None:intergenic
AAGTTGAAATGACAGAACAG+TGG 0.697944 3_3:-62096010 Msa0462840:CDS
ATCTCCTCGGAACTCTACAG+CGG 0.720267 3_3:-62098317 Msa0462840:CDS
AGAGACGCGTGAATTGAACA+TGG 0.743408 3_3:+62095752 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAAAAAAAAAATGACT+AGG - chr3_3:62096330-62096349 Msa0462840:intron 10.0%
!!! ATTAACATTGTTTCTTAATT+AGG - chr3_3:62095880-62095899 Msa0462840:CDS 15.0%
!! TCAAAAATCAAGTAAATTAT+TGG - chr3_3:62096443-62096462 Msa0462840:intron 15.0%
!! CAAAAATCAAGTAAATTATT+GGG - chr3_3:62096444-62096463 Msa0462840:intron 15.0%
!!! CACTTTTTGTTTTTAAATTA+CGG - chr3_3:62096971-62096990 Msa0462840:intron 15.0%
!!! TTTTAGAAAAACATGTTATA+TGG + chr3_3:62096998-62097017 None:intergenic 15.0%
!!! TTAATTCTTTTATTCCATTT+TGG - chr3_3:62097310-62097329 Msa0462840:intron 15.0%
!!! TAATTCTTTTATTCCATTTT+GGG - chr3_3:62097311-62097330 Msa0462840:intron 15.0%
!!! AGTTAACTGTTTTATTTTTT+TGG - chr3_3:62097771-62097790 Msa0462840:intron 15.0%
!! AAGTTATAACTTATAATAAC+AGG + chr3_3:62098317-62098336 None:intergenic 15.0%
!!! CTTTTTCATAACAAAATACT+AGG - chr3_3:62096188-62096207 Msa0462840:intron 20.0%
!!! TGATTTTTGAATAATAGAAC+AGG + chr3_3:62096433-62096452 None:intergenic 20.0%
!!! AAATTAAGTTGTTTATCCAA+AGG - chr3_3:62096728-62096747 Msa0462840:intron 20.0%
!!! GTTAAACTACTATAATCTTT+TGG - chr3_3:62097365-62097384 Msa0462840:intron 20.0%
!!! CATTATCTTATTTAATAGAC+TGG - chr3_3:62097389-62097408 Msa0462840:intron 20.0%
!!! TGTGGTTGAATTTTTTATAT+TGG - chr3_3:62097493-62097512 Msa0462840:intron 20.0%
!!! TATTTATAGAAGTATAAGGA+TGG + chr3_3:62097704-62097723 None:intergenic 20.0%
!!! GAAGTATTTATAGAAGTATA+AGG + chr3_3:62097708-62097727 None:intergenic 20.0%
!! CTTCTATAAATACTTCTAAT+TGG - chr3_3:62097712-62097731 Msa0462840:intron 20.0%
!!! TGCAAAAATATTTTTGTTGT+TGG - chr3_3:62098213-62098232 Msa0462840:intron 20.0%
! TTTCTTAATTAGGTGTGTAA+TGG - chr3_3:62095890-62095909 Msa0462840:CDS 25.0%
! TTCTTAATTAGGTGTGTAAT+GGG - chr3_3:62095891-62095910 Msa0462840:CDS 25.0%
!!! CAAAGCATATCATTTTTATC+TGG + chr3_3:62096244-62096263 None:intergenic 25.0%
!! TTGTGTAGTAAATAGTTTGT+AGG - chr3_3:62096261-62096280 Msa0462840:intron 25.0%
! TATCATAATTGTGCTTGAAT+TGG + chr3_3:62096288-62096307 None:intergenic 25.0%
!! TTTTTGAATAATAGAACAGG+AGG + chr3_3:62096430-62096449 None:intergenic 25.0%
!! GTTTTCAGCTTATCTTTATA+AGG - chr3_3:62096609-62096628 Msa0462840:intron 25.0%
! TTATCTTTATAAGGTCTCAA+GGG - chr3_3:62096618-62096637 Msa0462840:intron 25.0%
!! TAAACAAATGTTTCTACCTT+TGG + chr3_3:62096747-62096766 None:intergenic 25.0%
!!! TTTGCTTTTATGTGATGTTA+CGG - chr3_3:62096892-62096911 Msa0462840:intron 25.0%
! CTGCGATATAAAAACATTTA+GGG + chr3_3:62097166-62097185 None:intergenic 25.0%
! GAAAGTCTGAAAATTGATAA+TGG - chr3_3:62097273-62097292 Msa0462840:intron 25.0%
! CCAAATTTACATGAATCAAA+AGG + chr3_3:62097454-62097473 None:intergenic 25.0%
!!! CCTTTTGATTCATGTAAATT+TGG - chr3_3:62097451-62097470 Msa0462840:intron 25.0%
! TGTAAATTTGGAAAACATCT+TGG - chr3_3:62097463-62097482 Msa0462840:intron 25.0%
!!! GTTGAATTTTTTATATTGGC+TGG - chr3_3:62097497-62097516 Msa0462840:intron 25.0%
! GATACTTCCTAAATTATGTT+TGG + chr3_3:62098258-62098277 None:intergenic 25.0%
! TTCTTATTGTCTTCTCATTT+GGG - chr3_3:62098357-62098376 Msa0462840:CDS 25.0%
ATGTTCGTGTAAAGATGATT+TGG - chr3_3:62095786-62095805 Msa0462840:three_prime_UTR 30.0%
AATGGGTATAGACGAATATA+AGG - chr3_3:62096487-62096506 Msa0462840:intron 30.0%
CTTATCTTTATAAGGTCTCA+AGG - chr3_3:62096617-62096636 Msa0462840:intron 30.0%
!! ACAACTTAATTTGCAGCTTA+TGG + chr3_3:62096720-62096739 None:intergenic 30.0%
AACAATATGAGAGCTTACAA+AGG + chr3_3:62096848-62096867 None:intergenic 30.0%
CATATTGTTTCCCTATTAGA+AGG - chr3_3:62096859-62096878 Msa0462840:intron 30.0%
GATTCTACTTCCTTCTAATA+GGG + chr3_3:62096872-62096891 None:intergenic 30.0%
AGATTCTACTTCCTTCTAAT+AGG + chr3_3:62096873-62096892 None:intergenic 30.0%
! GAAACAGTTTAGTCTTCTAA+GGG - chr3_3:62096914-62096933 Msa0462840:intron 30.0%
! AAAAACATTTAGGGTGTGTT+TGG + chr3_3:62097157-62097176 None:intergenic 30.0%
CCTGCGATATAAAAACATTT+AGG + chr3_3:62097167-62097186 None:intergenic 30.0%
!! CCTAAATGTTTTTATATCGC+AGG - chr3_3:62097164-62097183 Msa0462840:intron 30.0%
AGTTGAATCAAATCACAACT+AGG + chr3_3:62097249-62097268 None:intergenic 30.0%
!!! CTTTTATTCCATTTTGGGTT+TGG - chr3_3:62097316-62097335 Msa0462840:intron 30.0%
! CAAAAGGTTACTCTTTTCTA+TGG + chr3_3:62097438-62097457 None:intergenic 30.0%
! GCAATATTTTACCATCAAAC+AGG + chr3_3:62097542-62097561 None:intergenic 30.0%
ATGCTTGAATTCACATACTA+TGG + chr3_3:62097593-62097612 None:intergenic 30.0%
!!! ATTTGTCACTTTTCTACCTT+TGG + chr3_3:62097663-62097682 None:intergenic 30.0%
! AAAAGTGACAAATGTTGGTT+AGG - chr3_3:62097670-62097689 Msa0462840:intron 30.0%
TCCTAAAATCCTATAACTTC+AGG - chr3_3:62098050-62098069 Msa0462840:intron 30.0%
! TCCTGAAGTTATAGGATTTT+AGG + chr3_3:62098054-62098073 None:intergenic 30.0%
TGTTCAAGTAAGTAACAAGA+AGG - chr3_3:62098182-62098201 Msa0462840:intron 30.0%
GTTCAAGTAAGTAACAAGAA+GGG - chr3_3:62098183-62098202 Msa0462840:intron 30.0%
! TCATATTTTTCACAACATGC+AGG - chr3_3:62098278-62098297 Msa0462840:CDS 30.0%
CTTCTTATTGTCTTCTCATT+TGG - chr3_3:62098356-62098375 Msa0462840:CDS 30.0%
AATCTTCATGTCATGGCTTT+GGG + chr3_3:62095464-62095483 None:intergenic 35.0%
AGTTGAGAATCTTCATGTCA+TGG + chr3_3:62095471-62095490 None:intergenic 35.0%
! TGCATTTTCAATGTTGTCGA+CGG + chr3_3:62095551-62095570 None:intergenic 35.0%
ACATTGAAAATGCATCTCCT+CGG - chr3_3:62095557-62095576 Msa0462840:three_prime_UTR 35.0%
! ACTGTGGTTAATGTTGATGA+TGG - chr3_3:62095670-62095689 Msa0462840:three_prime_UTR 35.0%
TAGGATTTGTCATCTCAGTA+TGG - chr3_3:62095708-62095727 Msa0462840:three_prime_UTR 35.0%
ATGATGACCAACATGAATCT+GGG - chr3_3:62095746-62095765 Msa0462840:three_prime_UTR 35.0%
ATAAACAATGTGCTGAAGCT+TGG - chr3_3:62095818-62095837 Msa0462840:three_prime_UTR 35.0%
!! TGTAGCATTGACACTTATGA+TGG - chr3_3:62095974-62095993 Msa0462840:CDS 35.0%
ACTCACTATTAGTGCTTCAT+AGG - chr3_3:62096090-62096109 Msa0462840:intron 35.0%
ACATGAGTGACTCAAATAGT+AGG + chr3_3:62096125-62096144 None:intergenic 35.0%
TGACATGTGCTGAATATTCT+CGG - chr3_3:62096144-62096163 Msa0462840:intron 35.0%
AATATTCTCGGAGGTTTAGA+TGG - chr3_3:62096156-62096175 Msa0462840:intron 35.0%
! TACCCATTTTACCCTTTTCT+GGG + chr3_3:62096475-62096494 None:intergenic 35.0%
! ATACCCATTTTACCCTTTTC+TGG + chr3_3:62096476-62096495 None:intergenic 35.0%
ATGATTGTCTGAGAGAACTT+AGG - chr3_3:62096558-62096577 Msa0462840:intron 35.0%
TTGTTTAGATAACCAGAGCT+AGG - chr3_3:62096760-62096779 Msa0462840:intron 35.0%
TGTTTAGATAACCAGAGCTA+GGG - chr3_3:62096761-62096780 Msa0462840:intron 35.0%
TATCAAACATGATGTGCACT+GGG + chr3_3:62096809-62096828 None:intergenic 35.0%
ATATCAAACATGATGTGCAC+TGG + chr3_3:62096810-62096829 None:intergenic 35.0%
! GGAAACAGTTTAGTCTTCTA+AGG - chr3_3:62096913-62096932 Msa0462840:intron 35.0%
! TGTGTTTGGATTGATGGTAT+AGG - chr3_3:62096937-62096956 Msa0462840:intron 35.0%
! TTTGGATTGATGGTATAGGA+GGG - chr3_3:62096941-62096960 Msa0462840:intron 35.0%
TTTATATCGCAGGTACTTAG+AGG - chr3_3:62097174-62097193 Msa0462840:intron 35.0%
! TCCAACTTTTATGTGACAGT+TGG + chr3_3:62097415-62097434 None:intergenic 35.0%
AAACATCTTGGAAACTCTTG+TGG - chr3_3:62097475-62097494 Msa0462840:intron 35.0%
ATTGGCTGGATTAATTCTCA+TGG - chr3_3:62097511-62097530 Msa0462840:intron 35.0%
AAGTAGTTGTTCAAGTCCAA+AGG - chr3_3:62097644-62097663 Msa0462840:intron 35.0%
GGTAGAAAAGTGACAAATGT+TGG - chr3_3:62097665-62097684 Msa0462840:intron 35.0%
! ATAGAAGTATAAGGATGGCT+TGG + chr3_3:62097699-62097718 None:intergenic 35.0%
TACAGAACTTGTGAAATCTG+TGG - chr3_3:62097822-62097841 Msa0462840:intron 35.0%
TCCATTGCTAGAAATGTATC+TGG - chr3_3:62097846-62097865 Msa0462840:intron 35.0%
!! ACCAGATACATTTCTAGCAA+TGG + chr3_3:62097850-62097869 None:intergenic 35.0%
AAGTTGAAATGACAGAACAG+TGG - chr3_3:62097877-62097896 Msa0462840:intron 35.0%
GTAAGATGATTGAGGATTTG+TGG - chr3_3:62098082-62098101 Msa0462840:intron 35.0%
TCTGTGACCATTATAAACAC+AGG - chr3_3:62098110-62098129 Msa0462840:intron 35.0%
CATGGTACCTGTGTTTATAA+TGG + chr3_3:62098120-62098139 None:intergenic 35.0%
CCTAAATTATGTTTGGAAGC+TGG + chr3_3:62098251-62098270 None:intergenic 35.0%
CCAGCTTCCAAACATAATTT+AGG - chr3_3:62098248-62098267 Msa0462840:CDS 35.0%
AATAAGAAGCAGTTGAGACA+AGG + chr3_3:62098345-62098364 None:intergenic 35.0%
! CTTCTCATTTGGGTTATGAA+TGG - chr3_3:62098367-62098386 Msa0462840:CDS 35.0%
GAATCTTCATGTCATGGCTT+TGG + chr3_3:62095465-62095484 None:intergenic 40.0%
! GCATTTTCAATGTTGTCGAC+GGG + chr3_3:62095550-62095569 None:intergenic 40.0%
GTCTGAATGTTGTGTGGATT+TGG - chr3_3:62095639-62095658 Msa0462840:three_prime_UTR 40.0%
ATGTTGTGTGGATTTGGATC+TGG - chr3_3:62095645-62095664 Msa0462840:three_prime_UTR 40.0%
ATGGTGTGAAGAGAGATTGT+AGG - chr3_3:62095689-62095708 Msa0462840:three_prime_UTR 40.0%
GATGATGACCAACATGAATC+TGG - chr3_3:62095745-62095764 Msa0462840:three_prime_UTR 40.0%
CACGAACATCCTAGTTCAAT+GGG + chr3_3:62095775-62095794 None:intergenic 40.0%
ACACGAACATCCTAGTTCAA+TGG + chr3_3:62095776-62095795 None:intergenic 40.0%
ATGTGCTGAAGCTTGGTTTA+AGG - chr3_3:62095825-62095844 Msa0462840:three_prime_UTR 40.0%
! TGAAGCTTGGTTTAAGGTCA+AGG - chr3_3:62095831-62095850 Msa0462840:three_prime_UTR 40.0%
! GAAGCTTGGTTTAAGGTCAA+GGG - chr3_3:62095832-62095851 Msa0462840:three_prime_UTR 40.0%
! ACTAGGTTGAGACATGCTAT+TGG - chr3_3:62096205-62096224 Msa0462840:intron 40.0%
AAGTGAAACTTGCAAGATGC+AGG + chr3_3:62096400-62096419 None:intergenic 40.0%
TGTCCCAGAAAAGGGTAAAA+TGG - chr3_3:62096469-62096488 Msa0462840:intron 40.0%
GTCCCAGAAAAGGGTAAAAT+GGG - chr3_3:62096470-62096489 Msa0462840:intron 40.0%
TAGGGACGAAAATTCAGTAG+AGG - chr3_3:62096779-62096798 Msa0462840:intron 40.0%
! TAGTCTTCTAAGGGTGTGTT+TGG - chr3_3:62096923-62096942 Msa0462840:intron 40.0%
! TAAGGGTGTGTTTGGATTGA+TGG - chr3_3:62096931-62096950 Msa0462840:intron 40.0%
! GTTTGGATTGATGGTATAGG+AGG - chr3_3:62096940-62096959 Msa0462840:intron 40.0%
! TTGGATTGATGGTATAGGAG+GGG - chr3_3:62096942-62096961 Msa0462840:intron 40.0%
! ACATTTAGGGTGTGTTTGGA+TGG + chr3_3:62097153-62097172 None:intergenic 40.0%
ATGGACTTCATCTGGAGAAT+GGG - chr3_3:62097197-62097216 Msa0462840:intron 40.0%
GCCAACTGTCACATAAAAGT+TGG - chr3_3:62097411-62097430 Msa0462840:intron 40.0%
! TCATGGCTTTACCTGTTTGA+TGG - chr3_3:62097528-62097547 Msa0462840:intron 40.0%
CAAGGAGGAAATTCAAGAAC+CGG + chr3_3:62097977-62097996 None:intergenic 40.0%
AAAAAGCCAGGAGATGACAA+AGG + chr3_3:62098015-62098034 None:intergenic 40.0%
CAAATAGAAGCGAAAAAGCC+AGG + chr3_3:62098027-62098046 None:intergenic 40.0%
CTGCTAGATCCTGAAGTTAT+AGG + chr3_3:62098062-62098081 None:intergenic 40.0%
CTATAACTTCAGGATCTAGC+AGG - chr3_3:62098060-62098079 Msa0462840:intron 40.0%
TCTAGCAGGTAAGATGATTG+AGG - chr3_3:62098074-62098093 Msa0462840:intron 40.0%
ATTGAGACTGCATTGTGTTG+AGG - chr3_3:62098414-62098433 Msa0462840:CDS 40.0%
TGCATTGTGTTGAGGAAATG+AGG - chr3_3:62098422-62098441 Msa0462840:CDS 40.0%
! TGCGTGAAGGACATTTTCGA+TGG + chr3_3:62095500-62095519 None:intergenic 45.0%
ATCGAAAATGTCCTTCACGC+AGG - chr3_3:62095499-62095518 Msa0462840:three_prime_UTR 45.0%
AACTCTACAGCGGAGAATGT+TGG - chr3_3:62095580-62095599 Msa0462840:three_prime_UTR 45.0%
GTCTGTGTCTGAATGTTGTG+TGG - chr3_3:62095633-62095652 Msa0462840:three_prime_UTR 45.0%
CACATGTGTCAGTGTTGTGT+CGG + chr3_3:62096009-62096028 None:intergenic 45.0%
CATGTGCTGAATATTCTCGG+AGG - chr3_3:62096147-62096166 Msa0462840:intron 45.0%
AGGTGCATCAGCATTTGCAT+AGG - chr3_3:62096350-62096369 Msa0462840:intron 45.0%
TATTGGGTGTGTCCCAGAAA+AGG - chr3_3:62096460-62096479 Msa0462840:intron 45.0%
ATTGGGTGTGTCCCAGAAAA+GGG - chr3_3:62096461-62096480 Msa0462840:intron 45.0%
! TGAATTTTCGTCCCTAGCTC+TGG + chr3_3:62096775-62096794 None:intergenic 45.0%
! ATTGATGGTATAGGAGGGGA+AGG - chr3_3:62096946-62096965 Msa0462840:intron 45.0%
TTTGGATGGAGGGTTTAGGA+GGG + chr3_3:62097139-62097158 None:intergenic 45.0%
TGTGTTTGGATGGAGGGTTT+AGG + chr3_3:62097143-62097162 None:intergenic 45.0%
TTAGGGTGTGTTTGGATGGA+GGG + chr3_3:62097149-62097168 None:intergenic 45.0%
! TTTAGGGTGTGTTTGGATGG+AGG + chr3_3:62097150-62097169 None:intergenic 45.0%
TATCGCAGGTACTTAGAGGA+TGG - chr3_3:62097178-62097197 Msa0462840:intron 45.0%
! CTTAGAGGATGGACTTCATC+TGG - chr3_3:62097189-62097208 Msa0462840:intron 45.0%
! GATGGACTTCATCTGGAGAA+TGG - chr3_3:62097196-62097215 Msa0462840:intron 45.0%
GGTCAACTCCAAACCCAAAA+TGG + chr3_3:62097327-62097346 None:intergenic 45.0%
AACAGAGCAGAGTGCACAAT+TGG + chr3_3:62097348-62097367 None:intergenic 45.0%
!! AGTAGGCTTTGAGTTTCTGC+AGG + chr3_3:62097951-62097970 None:intergenic 45.0%
AAACTCAAAGCCTACTCCAC+CGG - chr3_3:62097955-62097974 Msa0462840:intron 45.0%
AATTCAAGAACCGGTGGAGT+AGG + chr3_3:62097968-62097987 None:intergenic 45.0%
GAATTTCCTCCTTGCCTGTA+TGG - chr3_3:62097983-62098002 Msa0462840:intron 45.0%
GACAAAGGCAATGACCATAC+AGG + chr3_3:62098000-62098019 None:intergenic 45.0%
TCATTGCCTTTGTCATCTCC+TGG - chr3_3:62098006-62098025 Msa0462840:intron 45.0%
AGAGACGCGTGAATTGAACA+TGG + chr3_3:62098138-62098157 None:intergenic 45.0%
!!! TTTTTAATTATTTAGAAAAA+GGG + chr3_3:62096319-62096338 None:intergenic 5.0%
!!! TTTTTTAATTATTTAGAAAA+AGG + chr3_3:62096320-62096339 None:intergenic 5.0%
ACTTGTCTTGTCCTGCGTGA+AGG + chr3_3:62095513-62095532 None:intergenic 50.0%
ATCTCCTCGGAACTCTACAG+CGG - chr3_3:62095570-62095589 Msa0462840:three_prime_UTR 50.0%
GGATTTGGATCTGGAGACTG+TGG - chr3_3:62095654-62095673 Msa0462840:three_prime_UTR 50.0%
TGGGAGTCCCAGATTCATGT+TGG + chr3_3:62095756-62095775 None:intergenic 50.0%
! TCTGGGACTCCCATTGAACT+AGG - chr3_3:62095763-62095782 Msa0462840:three_prime_UTR 50.0%
TTGGATGGAGGGTTTAGGAG+GGG + chr3_3:62097138-62097157 None:intergenic 50.0%
GTTTGGATGGAGGGTTTAGG+AGG + chr3_3:62097140-62097159 None:intergenic 50.0%
! CTTTGAGTTTCTGCAGGTGG+GGG + chr3_3:62097945-62097964 None:intergenic 50.0%
! GCTTTGAGTTTCTGCAGGTG+GGG + chr3_3:62097946-62097965 None:intergenic 50.0%
!! GGCTTTGAGTTTCTGCAGGT+GGG + chr3_3:62097947-62097966 None:intergenic 50.0%
!! AGGCTTTGAGTTTCTGCAGG+TGG + chr3_3:62097948-62097967 None:intergenic 50.0%
GGAGGAAATTCAAGAACCGG+TGG + chr3_3:62097974-62097993 None:intergenic 50.0%
CAATGACCATACAGGCAAGG+AGG + chr3_3:62097992-62098011 None:intergenic 50.0%
AGGCAATGACCATACAGGCA+AGG + chr3_3:62097995-62098014 None:intergenic 50.0%
TTCTCCGCTGTAGAGTTCCG+AGG + chr3_3:62095577-62095596 None:intergenic 55.0%
TGGATGGAGGGTTTAGGAGG+GGG + chr3_3:62097137-62097156 None:intergenic 55.0%
TGGAGGGTTTAGGAGGGGGA+GGG + chr3_3:62097133-62097152 None:intergenic 60.0%
ATGGAGGGTTTAGGAGGGGG+AGG + chr3_3:62097134-62097153 None:intergenic 60.0%
TGAGTTTCTGCAGGTGGGGG+AGG + chr3_3:62097942-62097961 None:intergenic 60.0%
GGTTTAGGAGGGGGAGGGAG+GGG + chr3_3:62097128-62097147 None:intergenic 65.0%
GGGTTTAGGAGGGGGAGGGA+GGG + chr3_3:62097129-62097148 None:intergenic 65.0%
AGGGTTTAGGAGGGGGAGGG+AGG + chr3_3:62097130-62097149 None:intergenic 65.0%
GGTGGGGGAGGTCTAGTTGG+TGG + chr3_3:62097930-62097949 None:intergenic 65.0%
GCAGGTGGGGGAGGTCTAGT+TGG + chr3_3:62097933-62097952 None:intergenic 65.0%
TAGGAGGGGGAGGGAGGGGA+GGG + chr3_3:62097124-62097143 None:intergenic 70.0%
TTAGGAGGGGGAGGGAGGGG+AGG + chr3_3:62097125-62097144 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 62095448 62098461 62095448 ID=Msa0462840;Name=Msa0462840
chr3_3 mRNA 62095448 62098461 62095448 ID=Msa0462840-mRNA-1;Parent=Msa0462840;Name=Msa0462840-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0.33|1|0|0.33|3|406|159
chr3_3 exon 62095448 62096082 62095448 ID=Msa0462840-mRNA-1:exon:13230;Parent=Msa0462840-mRNA-1
chr3_3 exon 62098203 62098209 62098203 ID=Msa0462840-mRNA-1:exon:13229;Parent=Msa0462840-mRNA-1
chr3_3 exon 62098218 62098461 62098218 ID=Msa0462840-mRNA-1:exon:13228;Parent=Msa0462840-mRNA-1
chr3_3 CDS 62098218 62098461 62098218 ID=Msa0462840-mRNA-1:cds;Parent=Msa0462840-mRNA-1
chr3_3 CDS 62098203 62098209 62098203 ID=Msa0462840-mRNA-1:cds;Parent=Msa0462840-mRNA-1
chr3_3 CDS 62095854 62096082 62095854 ID=Msa0462840-mRNA-1:cds;Parent=Msa0462840-mRNA-1
chr3_3 three_prime_UTR 62095448 62095853 62095448 ID=Msa0462840-mRNA-1:three_prime_utr;Parent=Msa0462840-mRNA-1
Gene Sequence

>Msa0462840

ATGATCTCTATCTCCCAAAGCCATGACATGAAGATTCTCAACTTTAGTACCATCGAAAATGTCCTTCACGCAGGACAAGACAAGTCTCACGTTGTTGTTCCCGTCGACAACATTGAAAATGCATCTCCTCGGAACTCTACAGCGGAGAATGTTGGAGTGTGTGAGAAAGAGAGAGTGTCTTCTGTGTCTGTGTCTGAATGTTGTGTGGATTTGGATCTGGAGACTGTGGTTAATGTTGATGATGAGAGATTAACTTGTGAAATCTGTGGATCCATTGCTAGAAATGTATCTGGTATAGTTGAAGTTGAAATGACAGAACAGTGGAGTGAAGCTAATGATGCATCTGTTCTACCACCAACTAGACCTCCCCCACCTGCAGAAACTCAAAGCCTACTCCACCGGTTCTTGAATTTCCTCCTTGCCTGTATGGTCATTGCCTTTGTCATCTCCTGGCTTTTTCGCTTCTATTTGCATTCCTAA
Protein sequence

>Msa0462840

MISISQSHDMKILNFSTIENVLHAGQDKSHVVVPVDNIENASPRNSTAENVGVCEKERVSSVSVSECCVDLDLETVVNVDDERLTCEICGSIARNVSGIVEVEMTEQWSEANDASVLPPTRPPPPAETQSLLHRFLNFLLACMVIAFVISWLFRFYLHS*