AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466090 MtrunA17_Chr4g0002781 80.952 126 24 0 2 127 204 329 1.67e-67 207
Msa1466090 MtrunA17_Chr4g0002801 76.154 130 28 1 1 127 181 310 3.57e-64 199
Msa1466090 MtrunA17_Chr2g0333621 51.587 126 59 1 2 127 168 291 2.26e-34 121
Msa1466090 MtrunA17_Chr2g0333641 50.000 126 58 3 2 127 167 287 1.36e-30 112
Msa1466090 MtrunA17_Chr7g0225901 41.727 139 72 5 2 137 118 250 6.69e-27 100
Msa1466090 MtrunA17_Chr8g0336681 44.697 132 67 2 1 127 185 315 9.11e-27 102
Msa1466090 MtrunA17_Chr5g0400621 44.186 129 69 1 2 130 187 312 1.07e-25 99.4
Msa1466090 MtrunA17_Chr4g0054581 45.385 130 64 3 2 127 228 354 2.74e-24 96.3
Msa1466090 MtrunA17_Chr2g0333611 43.511 131 59 4 2 127 75 195 4.69e-24 92.8
Msa1466090 MtrunA17_Chr7g0233441 41.406 128 58 3 2 127 64 176 8.28e-24 91.7
Msa1466090 MtrunA17_Chr5g0400641 40.945 127 70 2 2 127 28 150 1.46e-22 87.4
Msa1466090 MtrunA17_Chr5g0400661 40.441 136 71 2 2 127 179 314 2.03e-22 90.5
Msa1466090 MtrunA17_Chr4g0002831 41.860 129 56 3 2 127 72 184 2.51e-22 87.8
Msa1466090 MtrunA17_Chr4g0002851 39.683 126 55 2 2 127 72 176 5.31e-21 84.3
Msa1466090 MtrunA17_Chr4g0054561 44.094 127 64 4 2 127 185 305 9.13e-21 85.9
Msa1466090 MtrunA17_Chr4g0033391 41.406 128 65 3 2 127 177 296 9.72e-21 85.9
Msa1466090 MtrunA17_Chr6g0468491 38.281 128 72 2 2 127 167 289 1.64e-20 85.1
Msa1466090 MtrunA17_Chr2g0307131 39.683 126 73 2 2 127 209 331 1.01e-19 83.6
Msa1466090 MtrunA17_Chr4g0033291 39.844 128 67 3 2 127 185 304 4.56e-19 81.3
Msa1466090 MtrunA17_Chr2g0307141 38.889 126 74 2 2 127 213 335 7.63e-19 81.3
Msa1466090 MtrunA17_Chr4g0054551 42.520 127 66 4 2 127 185 305 8.15e-19 80.9
Msa1466090 MtrunA17_Chr2g0280161 41.538 130 70 3 2 129 207 332 2.67e-18 79.7
Msa1466090 MtrunA17_Chr4g0033281 39.683 126 69 2 2 127 183 301 6.25e-17 75.5
Msa1466090 MtrunA17_Chr4g0049331 36.641 131 74 3 2 129 165 289 2.13e-16 73.9
Msa1466090 MtrunA17_Chr5g0400671 35.714 126 75 1 2 127 184 303 3.31e-16 73.6
Msa1466090 MtrunA17_Chr7g0249811 34.127 126 71 1 2 127 195 308 2.45e-14 68.6
Msa1466090 MtrunA17_Chr4g0054621 35.156 128 69 3 2 127 202 317 2.53e-14 68.6
Msa1466090 MtrunA17_Chr4g0054571 37.879 132 74 3 2 127 180 309 5.08e-14 67.4
Msa1466090 MtrunA17_Chr6g0468461 32.540 126 68 2 2 127 203 311 1.15e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466090 AT4G37850.2 41.600 125 71 2 2 126 182 304 9.18e-21 86.3
Msa1466090 AT4G37850.1 41.600 125 71 2 2 126 182 304 9.18e-21 86.3
Msa1466090 AT2G22760.2 38.194 144 72 5 1 136 148 282 6.74e-19 80.9
Msa1466090 AT2G22760.1 37.778 135 68 4 1 127 148 274 8.77e-18 77.8
Msa1466090 AT2G22770.1 37.589 141 72 4 1 127 161 299 1.75e-16 74.7
Msa1466090 AT2G22750.3 42.188 128 69 3 2 126 184 309 1.20e-14 69.7
Msa1466090 AT2G22750.4 42.188 128 69 3 2 126 184 309 1.22e-14 69.7
Msa1466090 AT2G22750.2 42.188 128 69 3 2 126 156 281 1.37e-14 69.3
Msa1466090 AT2G22750.5 42.969 128 68 3 2 126 180 305 1.45e-14 69.3
Msa1466090 AT2G22750.1 42.188 128 69 3 2 126 156 281 1.49e-14 69.3

Find 20 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGACTCTTGCTTCAATATC+TGG 0.292442 tig0045893:-780 Msa1466090:intergenic
ATCCATTGTGAGAAACAAAA+TGG 0.294137 tig0045893:+825 Msa1466090:CDS
GGTAGCAGTGTCTTGCCATT+TGG 0.333645 tig0045893:+900 Msa1466090:CDS
GTAGCAGTGTCTTGCCATTT+GGG 0.353594 tig0045893:+901 Msa1466090:CDS
CAATATCTGGAAGGATACTA+TGG 0.374591 tig0045893:-767 Msa1466090:intergenic
AGAAACAAGAAGAAAATGTT+GGG 0.406463 tig0045893:+652 Msa1466090:CDS
GAAATAGTTGATTTCCCAAA+TGG 0.414232 tig0045893:-915 Msa1466090:intergenic
TTCCATCACTATAATTGCTC+GGG 0.454181 tig0045893:+935 Msa1466090:CDS
GAGAAACAAGAAGAAAATGT+TGG 0.486811 tig0045893:+651 Msa1466090:CDS
TTTCCATCACTATAATTGCT+CGG 0.489893 tig0045893:+934 Msa1466090:CDS
GATATTGAAGCAAGAGTCAT+GGG 0.509819 tig0045893:+783 Msa1466090:CDS
AATCTTCAACTGTTTGTCAC+TGG 0.515596 tig0045893:+879 Msa1466090:CDS
TATTCATGTTCTTGATGAGT+TGG 0.536565 tig0045893:+977 Msa1466090:CDS
TACCCGAGCAATTATAGTGA+TGG 0.541857 tig0045893:-937 Msa1466090:intergenic
AGATATTGAAGCAAGAGTCA+TGG 0.568127 tig0045893:+782 Msa1466090:CDS
AAAGCTTCAATACTTCAAGC+AGG 0.588462 tig0045893:+585 Msa1466090:CDS
AACAAATCACAAAATTCATG+TGG 0.589942 tig0045893:+693 Msa1466090:CDS
CTCTTGCTTCAATATCTGGA+AGG 0.599921 tig0045893:-776 Msa1466090:intergenic
TGAAGCAAGAGTCATGGGAA+AGG 0.625639 tig0045893:+788 Msa1466090:CDS
GAAACAAGAAGAAAATGTTG+GGG 0.696078 tig0045893:+653 Msa1466090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAACAAGAAGAAAATGTT+GGG + tig0045893:652-671 Msa1466090:CDS 25.0%
!!! TGAATTTTGTGATTTGTTGA+GGG - tig0045893:691-710 Msa1466090:intergenic 25.0%
!!! ATGAATTTTGTGATTTGTTG+AGG - tig0045893:692-711 Msa1466090:intergenic 25.0%
! AACAAATCACAAAATTCATG+TGG + tig0045893:693-712 Msa1466090:CDS 25.0%
!! ACAGTTGAAGATTTTCAATA+TGG - tig0045893:872-891 Msa1466090:intergenic 25.0%
GAGAAACAAGAAGAAAATGT+TGG + tig0045893:651-670 Msa1466090:CDS 30.0%
GAAACAAGAAGAAAATGTTG+GGG + tig0045893:653-672 Msa1466090:CDS 30.0%
ATCCATTGTGAGAAACAAAA+TGG + tig0045893:825-844 Msa1466090:CDS 30.0%
!! TTCCATTTTGTTTCTCACAA+TGG - tig0045893:830-849 Msa1466090:intergenic 30.0%
! GAAATAGTTGATTTCCCAAA+TGG - tig0045893:918-937 Msa1466090:intergenic 30.0%
TTTCCATCACTATAATTGCT+CGG + tig0045893:934-953 Msa1466090:CDS 30.0%
TATTCATGTTCTTGATGAGT+TGG + tig0045893:977-996 Msa1466090:CDS 30.0%
AAAGCTTCAATACTTCAAGC+AGG + tig0045893:585-604 Msa1466090:CDS 35.0%
CAATATCTGGAAGGATACTA+TGG - tig0045893:770-789 Msa1466090:intergenic 35.0%
ATGACTCTTGCTTCAATATC+TGG - tig0045893:783-802 Msa1466090:intergenic 35.0%
AGATATTGAAGCAAGAGTCA+TGG + tig0045893:782-801 Msa1466090:CDS 35.0%
GATATTGAAGCAAGAGTCAT+GGG + tig0045893:783-802 Msa1466090:CDS 35.0%
AATCTTCAACTGTTTGTCAC+TGG + tig0045893:879-898 Msa1466090:CDS 35.0%
TTCCATCACTATAATTGCTC+GGG + tig0045893:935-954 Msa1466090:CDS 35.0%
CTCTTGCTTCAATATCTGGA+AGG - tig0045893:779-798 Msa1466090:intergenic 40.0%
TACCCGAGCAATTATAGTGA+TGG - tig0045893:940-959 Msa1466090:intergenic 40.0%
TGAAGCAAGAGTCATGGGAA+AGG + tig0045893:788-807 Msa1466090:CDS 45.0%
! GTAGCAGTGTCTTGCCATTT+GGG + tig0045893:901-920 Msa1466090:CDS 45.0%
GGTAGCAGTGTCTTGCCATT+TGG + tig0045893:900-919 Msa1466090:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0045893 gene 579 1013 579 ID=Msa1466090;Name=Msa1466090
tig0045893 mRNA 579 1013 579 ID=Msa1466090-mRNA-1;Parent=Msa1466090;Name=Msa1466090-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|144
tig0045893 exon 579 1013 579 ID=Msa1466090-mRNA-1:exon:24982;Parent=Msa1466090-mRNA-1
tig0045893 CDS 579 1013 579 ID=Msa1466090-mRNA-1:cds;Parent=Msa1466090-mRNA-1
Gene Sequence

>Msa1466090

ACAGACAAAGCTTCAATACTTCAAGCAGGAATTGATTATGTGAAACAGCTTAAAGAACGTGTTGAGAAGCTAGAGAAACAAGAAGAAAATGTTGGGGTCACATCAATGACCCTCAACAAATCACAAAATTCATGTGGAATTAATGAAGACACAAATTCTAGCGAAACAAGTTGTGATGATGATTGTAACCATAGTATCCTTCCAGATATTGAAGCAAGAGTCATGGGAAAGGAAGTGCTTATTGAAATCCATTGTGAGAAACAAAATGGAATTGAGCTCAAATTACTTAACCATATTGAAAATCTTCAACTGTTTGTCACTGGTAGCAGTGTCTTGCCATTTGGGAAATCAACTATTTCCATCACTATAATTGCTCGGGTAAGCATCAGTAAAGTGTATATTCATGTTCTTGATGAGTTGGAAGAAAAAAATTAA

Protein sequence

>Msa1466090

TDKASILQAGIDYVKQLKERVEKLEKQEENVGVTSMTLNKSQNSCGINEDTNSSETSCDDDCNHSILPDIEARVMGKEVLIEIHCEKQNGIELKLLNHIENLQLFVTGSSVLPFGKSTISITIIARVSISKVYIHVLDELEEKN*