AlfalfaGEDB | Msa0420650

M. sativa cultivar zhongmu-4 / Msa0420650

KEGG: K20776
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420650	XP_013461152.1	94.553	257	14	0	1	257	1	257	0.0	516

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420650	A0A072V0F2	94.553	257	14	0	1	257	1	257	0.0	516

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0022750	Msa0420650	0.815192	1.048343e-51	-8.615850e-47
Msa0035120	Msa0420650	-0.801716	7.897356e-49	-8.615850e-47
Msa0042550	Msa0420650	-0.801132	1.039921e-48	-8.615850e-47
Msa0055070	Msa0420650	-0.827671	1.375415e-54	-8.615850e-47
Msa0056450	Msa0420650	-0.823579	1.283780e-53	-8.615850e-47
Msa0080830	Msa0420650	-0.800251	1.573586e-48	-8.615850e-47
Msa0101020	Msa0420650	-0.827825	1.263311e-54	-8.615850e-47
Msa0103250	Msa0420650	-0.805938	1.048379e-49	-8.615850e-47
Msa0104800	Msa0420650	-0.800893	1.163925e-48	-8.615850e-47
Msa0106620	Msa0420650	-0.807478	4.956434e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420650	MtrunA17_Chr3g0120251	94.553	257	14	0	1	257	1	257	0.0	516

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420650	AT4G32960.1	66.383	235	76	2	11	245	16	247	1.47e-118	340
Msa0420650	AT4G32970.1	57.917	240	90	5	1	240	8	236	1.24e-90	281

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Find 73 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAAACGAGCGAAATGGA+AGG	0.579481	3_2:+69250495	Msa0420650:exon
AATTGCGTCTAAACGAGTGA+AGG	0.582630	3_2:-69250655	None:intergenic
ACAATAGAATTAACACATGA+CGG	0.580826	3_2:-69253080	None:intergenic
ACATTATCTTCAGTGAGCAT+TGG	0.548047	3_2:-69253180	None:intergenic
ACCTAAATCACTTGTGAAGA+AGG	0.463492	3_2:+69253143	Msa0420650:CDS
ACCTTCTTCACAAGTGATTT+AGG	0.250650	3_2:-69253144	None:intergenic
ACGAGTGAAGGGTCTTCCAT+TGG	0.502333	3_2:-69250643	None:intergenic
ACTTGTGAAGAAGGTTCCTC+AGG	0.543838	3_2:+69253152	Msa0420650:CDS
AGAAAACGAGCGAAATGGAA+GGG	0.583932	3_2:+69250496	Msa0420650:exon
AGAGATCTATGATTCACTGG+TGG	0.652124	3_2:+69252984	Msa0420650:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGTATGAGAAAGATGAGA+GGG	-	chr3_2:69250438-69250457	None:intergenic	30.0%
TTATGTATGAGAAAGATGAG+AGG	-	chr3_2:69250439-69250458	None:intergenic	30.0%
AAACATGCCATAATCTTTGA+GGG	-	chr3_2:69250897-69250916	None:intergenic	30.0%
GTCTTATAAGTTCATTTGAC+TGG	-	chr3_2:69251356-69251375	None:intergenic	30.0%
CAAATGAAAGAACATGAACT+AGG	-	chr3_2:69251591-69251610	None:intergenic	30.0%
AGAAAACGATTTAAGGAAAG+AGG	-	chr3_2:69251749-69251768	None:intergenic	30.0%
AAGCAGTTATCCATTTGATA+TGG	+	chr3_2:69251802-69251821	Msa0420650:intron	30.0%
ATCCCATTTACCATATCAAA+TGG	-	chr3_2:69251815-69251834	None:intergenic	30.0%
AGAAATTCATGAAAGATCCA+AGG	-	chr3_2:69251933-69251952	None:intergenic	30.0%
TTCCTAAAAGATTTGTACGT+AGG	+	chr3_2:69251997-69252016	Msa0420650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	69250321	69253399	69250321	ID=Msa0420650;Name=Msa0420650
chr3_2	mRNA	69250321	69253399	69250321	ID=Msa0420650-mRNA-1;Parent=Msa0420650;Name=Msa0420650-mRNA-1;_AED=0.06;_eAED=0.06;_QI=189\|1\|1\|1\|1\|1\|3\|181\|257
chr3_2	exon	69250321	69250770	69250321	ID=Msa0420650-mRNA-1:exon:16426;Parent=Msa0420650-mRNA-1
chr3_2	exon	69252546	69252704	69252546	ID=Msa0420650-mRNA-1:exon:16427;Parent=Msa0420650-mRNA-1
chr3_2	exon	69252865	69253399	69252865	ID=Msa0420650-mRNA-1:exon:16428;Parent=Msa0420650-mRNA-1
chr3_2	five_prime_UTR	69250321	69250509	69250321	ID=Msa0420650-mRNA-1:five_prime_utr;Parent=Msa0420650-mRNA-1
chr3_2	CDS	69250510	69250770	69250510	ID=Msa0420650-mRNA-1:cds;Parent=Msa0420650-mRNA-1
chr3_2	CDS	69252546	69252704	69252546	ID=Msa0420650-mRNA-1:cds;Parent=Msa0420650-mRNA-1
chr3_2	CDS	69252865	69253218	69252865	ID=Msa0420650-mRNA-1:cds;Parent=Msa0420650-mRNA-1
chr3_2	three_prime_UTR	69253219	69253399	69253219	ID=Msa0420650-mRNA-1:three_prime_utr;Parent=Msa0420650-mRNA-1

Gene Sequence

>Msa0420650

ATGGAAGGGGTTGAAGGAGAAAGAAGCAGCTTCAAACTCCAACCTTCACGAACCAATAATGAAGACATACTCTTCTGCATCGACATTGATCCACAATCCATGGCGGAGATGAAAGGTGCTACCAGTGGTAGACCCAATGGAAGACCCTTCACTCGTTTAGACGCAATTAAACAAGCCATAATTCTCTTTGTTAATGCTAAACTCACCATCAATCCTCAACATCGTTTCGCTTTCGCTACTCTCTCTGATTCCGTTTCTTGGCTGAAGAAAGAGTTTAGCAGTGAAGCTGAGTCAACAATTGCAGCAATGCGAGGACTTTCGGCTACTACATCTAGCAGTCAACCAGACCTTACTACTTTGTTCCGACTTGCTGCTCACGAAGCTAAGAAATCCCGTATGCAGGGTCGCATTTTAAGAGTGATTTTATTCTACTGCAGATCAACAGTGCGCCCACGACATCAGTGGCCTGTGAACCAGAAGCTCTTCACTTTTGATGTCATGTATCTTCATGACAAGCCTGGACCTGACAATTGCCCCCAAGAGATCTATGATTCACTGGTGGAAGCTCTTGAATATGTTACTGAATATGAGGGTTATATCTTGGAGAGTGGACAAGGGTTAGCAAGGGTTCTTTTCCGTCATGTGTTAATTCTATTGTGTCATCCTCAGCAGCGCTGCGCACAAGATTATATTGACATACCTAAATCACTTGTGAAGAAGGTTCCTCAGGCAGAGCCAATGCTCACTGAAGATAATGTTCCATCCAGCCAGTGA

Protein sequence

>Msa0420650

MEGVEGERSSFKLQPSRTNNEDILFCIDIDPQSMAEMKGATSGRPNGRPFTRLDAIKQAIILFVNAKLTINPQHRFAFATLSDSVSWLKKEFSSEAESTIAAMRGLSATTSSSQPDLTTLFRLAAHEAKKSRMQGRILRVILFYCRSTVRPRHQWPVNQKLFTFDVMYLHDKPGPDNCPQEIYDSLVEALEYVTEYEGYILESGQGLARVLFRHVLILLCHPQQRCAQDYIDIPKSLVKKVPQAEPMLTEDNVPSSQ*