AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0592390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592390 MtrunA17_Chr4g0010361 88.036 443 52 1 108 549 1 443 0.0 812
Msa0592390 MtrunA17_Chr4g0010361 82.301 113 11 3 1 105 290 401 1.72e-51 182
Msa0592390 MtrunA17_Chr4g0010331 87.783 442 48 1 108 549 1 436 0.0 806
Msa0592390 MtrunA17_Chr4g0010331 80.357 112 14 2 1 105 284 394 1.82e-53 187
Msa0592390 MtrunA17_Chr7g0224131 86.652 442 56 1 108 549 1 439 0.0 799
Msa0592390 MtrunA17_Chr7g0224131 79.464 112 12 3 1 105 290 397 1.54e-50 179
Msa0592390 MtrunA17_Chr4g0010311 84.163 442 70 0 108 549 1 442 0.0 781
Msa0592390 MtrunA17_Chr4g0010311 79.464 112 15 2 1 105 290 400 1.07e-51 182
Msa0592390 MtrunA17_Chr4g0010381 75.964 441 105 1 108 548 1 440 0.0 698
Msa0592390 MtrunA17_Chr4g0010381 69.643 112 26 2 1 105 289 399 6.40e-41 153
Msa0592390 MtrunA17_Chr4g0010351 83.957 374 60 0 108 481 1 374 0.0 659
Msa0592390 MtrunA17_Chr4g0010351 68.235 85 19 2 2 79 291 374 6.74e-29 118
Msa0592390 MtrunA17_Chr3g0125001 69.005 442 129 2 108 549 1 434 0.0 623
Msa0592390 MtrunA17_Chr3g0125001 54.867 113 43 2 1 105 280 392 5.76e-31 125
Msa0592390 MtrunA17_Chr3g0087221 67.261 449 140 2 108 549 1 449 0.0 612
Msa0592390 MtrunA17_Chr3g0087221 56.034 116 41 3 1 106 293 408 5.85e-32 128
Msa0592390 MtrunA17_Chr8g0354371 59.382 453 162 6 108 549 1 442 0.0 530
Msa0592390 MtrunA17_Chr8g0354371 56.897 116 37 4 1 107 289 400 3.92e-30 122
Msa0592390 MtrunA17_Chr8g0359251 58.186 452 168 7 108 548 1 442 0.0 529
Msa0592390 MtrunA17_Chr8g0359251 55.738 122 44 4 2 115 291 410 1.88e-33 132
Msa0592390 MtrunA17_Chr4g0053111 56.893 457 168 10 108 546 1 446 7.28e-170 488
Msa0592390 MtrunA17_Chr4g0053111 56.034 116 40 4 9 115 303 416 6.28e-29 119
Msa0592390 MtrunA17_Chr4g0053081 52.527 455 185 8 108 544 1 442 3.02e-161 466
Msa0592390 MtrunA17_Chr4g0053081 47.541 122 56 3 1 115 294 414 1.78e-25 109
Msa0592390 MtrunA17_Chr4g0053091 49.565 460 180 9 108 544 1 431 7.25e-146 427
Msa0592390 MtrunA17_Chr4g0053091 50.820 122 50 4 2 115 284 403 2.01e-27 115
Msa0592390 MtrunA17_Chr8g0374911 50.972 463 192 14 108 548 22 471 1.72e-144 424
Msa0592390 MtrunA17_Chr8g0374911 46.087 115 49 5 11 114 326 438 8.66e-18 86.3
Msa0592390 MtrunA17_Chr8g0374641 50.993 453 184 11 108 539 1 436 5.32e-144 422
Msa0592390 MtrunA17_Chr8g0374641 48.276 116 47 5 11 114 298 412 6.02e-23 101
Msa0592390 MtrunA17_Chr4g0053061 50.540 463 194 14 108 548 29 478 7.30e-144 423
Msa0592390 MtrunA17_Chr4g0053061 46.087 115 49 5 11 114 333 445 8.71e-18 86.3
Msa0592390 MtrunA17_Chr4g0010121 49.892 461 191 13 108 546 40 482 1.07e-141 418
Msa0592390 MtrunA17_Chr4g0010121 46.957 115 48 5 11 114 339 451 2.08e-17 85.1
Msa0592390 MtrunA17_Chr3g0134041 40.961 437 233 12 125 548 24 448 5.09e-96 299
Msa0592390 MtrunA17_Chr3g0134041 34.247 146 86 4 6 144 300 442 8.18e-15 77.0
Msa0592390 MtrunA17_Chr6g0456591 67.308 208 67 1 105 312 12 218 3.61e-93 284
Msa0592390 MtrunA17_Chr3g0134071 40.541 444 232 11 123 546 22 453 6.53e-93 293
Msa0592390 MtrunA17_Chr3g0134071 34.014 147 84 5 2 139 302 444 4.60e-13 71.6
Msa0592390 MtrunA17_Chr7g0224121 79.042 167 35 0 136 302 31 197 5.34e-92 280
Msa0592390 MtrunA17_Chr3g0134121 39.593 442 240 10 123 548 21 451 8.36e-90 285
Msa0592390 MtrunA17_Chr3g0134121 36.923 130 73 3 11 133 307 434 7.45e-14 73.9
Msa0592390 MtrunA17_Chr3g0134091 39.588 437 236 10 123 543 21 445 9.94e-88 278
Msa0592390 MtrunA17_Chr3g0134091 35.252 139 79 5 11 141 306 441 1.60e-12 69.7
Msa0592390 MtrunA17_Chr8g0353891 63.850 213 70 3 108 314 1 212 2.83e-87 268
Msa0592390 MtrunA17_Chr3g0134111 38.914 442 242 12 123 548 22 451 1.23e-85 273
Msa0592390 MtrunA17_Chr3g0134111 33.088 136 82 3 11 139 307 440 9.86e-12 67.4
Msa0592390 MtrunA17_Chr8g0353901 59.447 217 84 2 331 543 11 227 9.45e-81 253
Msa0592390 MtrunA17_Chr8g0353901 53.913 115 43 3 2 107 78 191 9.74e-28 112
Msa0592390 MtrunA17_Chr3g0134061 36.034 469 260 12 109 548 3 460 3.10e-77 251
Msa0592390 MtrunA17_Chr3g0134061 34.091 132 78 3 9 133 314 443 5.66e-14 74.3
Msa0592390 MtrunA17_Chr3g0134101 36.818 440 237 10 123 547 38 451 1.55e-74 243
Msa0592390 MtrunA17_Chr3g0134101 36.029 136 78 3 11 139 308 441 1.39e-14 76.3
Msa0592390 MtrunA17_Chr7g0224101 79.137 139 29 0 307 445 10 148 6.31e-73 229
Msa0592390 MtrunA17_Chr6g0463321 51.802 222 65 3 296 515 2 183 4.84e-66 212
Msa0592390 MtrunA17_Chr6g0463321 61.628 86 32 1 30 114 98 183 1.05e-27 109
Msa0592390 MtrunA17_Chr4g0005941 34.151 489 241 15 123 549 19 488 8.30e-63 214
Msa0592390 MtrunA17_Chr5g0436621 30.675 489 255 13 123 548 19 486 1.89e-58 202
Msa0592390 MtrunA17_Chr5g0436621 35.849 106 61 2 9 108 341 445 2.79e-11 65.9
Msa0592390 MtrunA17_Chr3g0113181 30.435 483 277 11 106 548 7 470 3.31e-57 198
Msa0592390 MtrunA17_Chr4g0053071 56.643 143 59 2 404 544 1 142 4.31e-49 166
Msa0592390 MtrunA17_Chr4g0053071 50.943 106 44 2 9 107 1 105 4.47e-27 106
Msa0592390 MtrunA17_Chr6g0463331 62.595 131 41 2 164 286 31 161 7.72e-47 161
Msa0592390 MtrunA17_Chr6g0467861 28.541 473 284 13 111 544 47 504 5.86e-45 166
Msa0592390 MtrunA17_Chr4g0053051 57.600 125 50 2 422 544 4 127 1.19e-41 146
Msa0592390 MtrunA17_Chr4g0053051 54.762 84 37 1 24 107 8 90 8.52e-22 91.3
Msa0592390 MtrunA17_Chr7g0224111 65.741 108 13 2 442 549 1 84 3.31e-37 132
Msa0592390 MtrunA17_Chr7g0224111 56.716 67 3 2 40 105 1 42 1.39e-12 63.5
Msa0592390 MtrunA17_Chr4g0001861 26.884 398 246 14 162 539 56 428 3.79e-30 122
Msa0592390 MtrunA17_Chr8g0371821 25.773 388 257 10 162 538 56 423 3.85e-29 119
Msa0592390 MtrunA17_Chr2g0334001 26.371 383 264 10 162 538 56 426 6.13e-29 119
Msa0592390 MtrunA17_Chr8g0386141 28.488 344 224 8 160 499 57 382 1.29e-26 112
Msa0592390 MtrunA17_Chr3g0087401 26.794 418 232 18 159 533 51 437 8.34e-24 104
Msa0592390 MtrunA17_Chr2g0311401 27.000 300 208 7 245 538 2 296 8.98e-23 99.0
Msa0592390 MtrunA17_Chr3g0132001 26.566 399 236 16 162 538 66 429 1.38e-22 100
Msa0592390 MtrunA17_Chr1g0165671 25.517 435 264 19 132 535 41 446 5.22e-20 93.2
Msa0592390 MtrunA17_Chr1g0191081 24.031 387 256 14 131 500 31 396 3.25e-19 90.5
Msa0592390 MtrunA17_Chr3g0134131 34.507 142 92 1 363 504 147 287 5.11e-19 88.2
Msa0592390 MtrunA17_Chr3g0134131 45.556 90 48 1 200 289 54 142 1.45e-15 78.2
Msa0592390 MtrunA17_Chr2g0323511 23.666 431 277 14 110 499 15 434 1.72e-18 88.6
Msa0592390 MtrunA17_Chr7g0224391 23.274 391 272 11 157 539 63 433 2.45e-18 87.8
Msa0592390 MtrunA17_Chr2g0323501 25.000 412 255 17 131 503 37 433 1.28e-17 85.9
Msa0592390 MtrunA17_Chr4g0054081 25.199 377 221 15 162 505 65 413 4.15e-17 84.0
Msa0592390 MtrunA17_Chr2g0323421 23.476 443 287 15 115 525 67 489 5.61e-17 84.0
Msa0592390 MtrunA17_Chr7g0252731 27.875 287 181 10 152 424 53 327 5.74e-17 83.6
Msa0592390 MtrunA17_Chr2g0323471 26.485 404 247 15 129 499 43 429 7.41e-17 83.6
Msa0592390 MtrunA17_Chr7g0273721 22.506 431 280 16 132 532 25 431 1.06e-16 82.8
Msa0592390 MtrunA17_Chr5g0433641 24.342 304 197 9 132 417 24 312 1.09e-16 82.8
Msa0592390 MtrunA17_Chr7g0219301 27.187 423 235 17 125 503 22 415 1.37e-16 83.6
Msa0592390 MtrunA17_Chr7g0219881 24.027 437 257 18 111 500 5 413 2.81e-16 81.6
Msa0592390 MtrunA17_Chr3g0123061 26.856 458 268 22 111 548 5 415 4.81e-16 80.5
Msa0592390 MtrunA17_Chr7g0219331 25.352 426 239 16 125 503 115 508 1.14e-15 80.1
Msa0592390 MtrunA17_Chr4g0027071 27.184 309 191 9 162 458 69 355 1.43e-15 79.3
Msa0592390 MtrunA17_Chr1g0165661 26.165 279 188 8 157 431 59 323 3.27e-15 78.2
Msa0592390 MtrunA17_Chr7g0219121 24.027 437 276 16 121 512 17 442 4.25e-15 77.8
Msa0592390 MtrunA17_Chr3g0113821 25.826 333 214 11 129 441 24 343 5.45e-15 77.4
Msa0592390 MtrunA17_Chr6g0454761 24.100 361 238 12 162 502 553 897 6.93e-15 78.2
Msa0592390 MtrunA17_Chr6g0454761 24.100 361 237 11 162 502 66 409 1.63e-13 73.6
Msa0592390 MtrunA17_Chr7g0273761 23.333 390 256 15 132 500 396 763 1.06e-14 77.4
Msa0592390 MtrunA17_Chr3g0124341 25.052 479 291 19 106 535 3 462 1.75e-14 75.9
Msa0592390 MtrunA17_Chr7g0253541 26.954 371 222 17 165 505 63 414 3.12e-14 75.1
Msa0592390 MtrunA17_Chr3g0083761 25.912 274 180 8 244 500 147 414 3.38e-14 75.1
Msa0592390 MtrunA17_Chr2g0306321 23.116 398 250 18 162 531 78 447 6.81e-14 73.9
Msa0592390 MtrunA17_Chr7g0219241 25.217 460 269 16 122 529 19 455 1.25e-13 73.2
Msa0592390 MtrunA17_Chr3g0097841 27.222 180 113 6 342 506 27 203 1.31e-13 70.9
Msa0592390 MtrunA17_Chr3g0106761 26.966 267 167 12 176 431 28 277 1.98e-13 72.4
Msa0592390 MtrunA17_Chr6g0455341 23.841 453 283 15 122 537 25 452 2.64e-13 72.4
Msa0592390 MtrunA17_Chr7g0253501 21.662 397 250 10 150 506 43 418 8.87e-13 70.5
Msa0592390 MtrunA17_Chr6g0470591 24.912 285 180 11 162 431 71 336 1.34e-12 70.1
Msa0592390 MtrunA17_Chr5g0442691 23.133 415 253 15 162 531 89 482 3.55e-12 68.9
Msa0592390 MtrunA17_Chr7g0253531 21.696 401 245 11 150 506 43 418 4.58e-12 68.2
Msa0592390 MtrunA17_Chr4g0004701 29.670 182 113 4 333 500 15 195 4.98e-12 66.2
Msa0592390 MtrunA17_Chr8g0361771 25.000 460 289 20 111 544 3 432 8.87e-12 67.4
Msa0592390 MtrunA17_Chr7g0219291 23.060 464 285 15 121 535 365 805 8.88e-12 68.2
Msa0592390 MtrunA17_Chr7g0219181 23.441 465 298 13 121 539 17 469 1.23e-11 67.0
Msa0592390 MtrunA17_Chr3g0106721 26.882 279 183 10 162 431 68 334 1.54e-11 66.6
Msa0592390 MtrunA17_Chr2g0283361 22.525 404 259 14 121 502 15 386 1.66e-11 66.6
Msa0592390 MtrunA17_Chr5g0442701 23.370 368 232 11 162 500 66 412 1.81e-11 66.6
Msa0592390 MtrunA17_Chr3g0106661 26.523 279 186 10 162 431 68 336 7.68e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592390 AT5G67150.1 39.693 456 248 12 108 547 1 445 9.03e-99 306
Msa0592390 AT5G67150.1 42.000 100 48 3 11 103 305 401 1.21e-15 79.7
Msa0592390 AT3G50300.1 41.818 440 222 15 108 529 1 424 1.59e-94 296
Msa0592390 AT3G50300.1 39.450 109 54 4 11 109 298 404 7.40e-14 74.3
Msa0592390 AT3G50280.1 37.862 449 257 10 108 544 1 439 6.94e-90 283
Msa0592390 AT3G50280.1 39.216 102 52 3 11 103 298 398 6.98e-13 71.2
Msa0592390 AT5G67160.1 37.838 444 245 10 113 548 12 432 1.97e-87 277
Msa0592390 AT5G67160.1 42.718 103 42 4 11 103 293 388 1.35e-13 73.6
Msa0592390 AT3G50270.1 37.611 452 256 11 113 548 7 448 1.17e-85 273
Msa0592390 AT3G50270.1 43.689 103 49 3 9 103 302 403 2.73e-14 75.9
Msa0592390 AT5G42830.1 37.273 440 252 10 122 548 21 449 1.68e-85 272
Msa0592390 AT5G38130.1 38.409 440 243 12 104 529 17 442 2.86e-85 272
Msa0592390 AT5G07850.1 40.000 445 238 13 122 549 22 454 1.64e-84 270
Msa0592390 AT5G07860.1 37.908 459 245 12 111 547 8 448 1.71e-83 267
Msa0592390 AT5G07860.1 37.500 104 55 3 11 107 307 407 2.41e-11 66.6
Msa0592390 AT5G07870.1 37.238 478 238 16 111 549 8 462 5.54e-76 248
Msa0592390 AT2G39980.1 33.472 481 246 13 123 549 21 481 3.63e-69 231
Msa0592390 AT2G39980.1 38.095 105 56 3 11 108 337 439 8.07e-12 68.2
Msa0592390 AT5G01210.1 34.681 470 244 12 123 548 19 469 3.20e-68 228
Msa0592390 AT5G01210.1 37.500 104 58 3 11 108 326 428 7.45e-12 68.2
Msa0592390 AT3G50280.2 37.931 319 182 6 232 544 1 309 1.75e-62 208
Msa0592390 AT3G50280.2 39.216 102 52 3 11 103 168 268 2.93e-13 71.2
Msa0592390 AT3G50290.1 41.606 274 147 6 229 497 4 269 9.59e-54 183
Msa0592390 AT5G23940.1 30.022 453 291 13 111 544 3 448 1.57e-49 178
Msa0592390 AT5G23940.1 39.806 103 55 3 7 103 305 406 1.27e-11 67.4
Msa0592390 AT5G48930.1 25.814 430 299 10 118 538 9 427 1.37e-31 127
Msa0592390 AT3G48720.1 26.980 404 259 14 149 538 44 425 3.29e-24 105
Msa0592390 AT5G57840.1 26.667 435 275 18 118 529 9 422 2.22e-22 100
Msa0592390 AT5G41040.2 24.439 401 265 13 159 546 66 441 1.11e-21 98.2
Msa0592390 AT5G41040.1 25.062 403 260 15 159 546 82 457 1.24e-21 98.2
Msa0592390 AT3G03480.1 27.007 411 245 18 132 518 33 412 1.78e-20 94.7
Msa0592390 AT3G29670.1 23.146 445 298 14 110 529 8 433 1.07e-19 92.4
Msa0592390 AT3G29590.1 25.272 459 281 15 111 535 8 438 5.48e-19 90.1
Msa0592390 AT5G63560.1 26.211 351 217 14 162 500 61 381 1.09e-18 89.0
Msa0592390 AT2G40230.1 24.719 445 303 16 108 539 1 426 8.00e-18 86.7
Msa0592390 AT1G65450.1 26.797 306 196 13 160 452 70 360 1.03e-17 86.3
Msa0592390 AT3G26040.1 25.424 413 248 14 158 548 55 429 1.41e-17 85.9
Msa0592390 AT5G23970.1 26.374 455 274 22 111 544 3 417 6.00e-17 84.0
Msa0592390 AT3G29635.1 24.828 435 272 15 128 529 26 438 5.28e-16 80.9
Msa0592390 AT1G28680.1 24.814 403 250 16 122 502 18 389 7.37e-16 80.5
Msa0592390 AT5G39090.1 26.087 276 170 10 242 499 140 399 9.67e-16 80.1
Msa0592390 AT3G29680.1 24.682 393 254 12 159 529 59 431 2.69e-15 79.0
Msa0592390 AT5G61160.1 23.881 402 249 15 127 500 31 403 5.71e-15 77.8
Msa0592390 AT1G28680.2 25.904 332 199 13 122 432 18 323 5.86e-15 77.4
Msa0592390 AT3G30280.1 25.304 411 249 20 161 548 61 436 7.04e-15 77.4
Msa0592390 AT1G27620.1 24.679 389 257 16 162 538 58 422 8.01e-15 77.4
Msa0592390 AT5G16410.1 24.510 408 234 18 148 521 77 444 2.41e-14 75.9
Msa0592390 AT5G07080.1 25.000 324 205 13 124 429 28 331 7.46e-14 74.3
Msa0592390 AT2G25150.1 24.722 360 240 12 157 503 62 403 1.05e-13 73.9
Msa0592390 AT1G03390.1 25.121 414 250 22 149 530 59 444 2.33e-13 72.8
Msa0592390 AT4G15390.1 22.837 416 248 17 160 544 62 435 2.35e-12 69.7
Msa0592390 AT4G15390.2 22.837 416 248 17 160 544 62 435 2.35e-12 69.7
Msa0592390 AT3G47170.1 27.667 300 177 14 151 429 64 344 4.88e-12 68.6
Msa0592390 AT5G39050.1 23.988 321 191 11 256 535 160 468 7.60e-12 68.2
Msa0592390 AT1G78990.1 24.507 355 231 16 160 500 65 396 2.71e-11 66.2

Find 114 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4_2:-17745052 Msa0592390:CDS
TCTCCATGGTTTGATGTTTA+TGG 0.113342 4_2:-17748128 Msa0592390:CDS
CCATTACCAGAAAATTATTT+TGG 0.134239 4_2:-17745283 Msa0592390:CDS
CCATTACCAGAATATTATTT+TGG 0.150661 4_2:-17748364 Msa0592390:CDS
ACTTGCTTCAACCAATAAAA+AGG 0.152814 4_2:-17746085 Msa0592390:CDS
TTAGCAAGCCTAACAAAATT+TGG 0.155332 4_2:+17745109 None:intergenic
ATAGTTTGATCACTTGATTT+TGG 0.265670 4_2:+17746138 None:intergenic
TTGTCTTCCACATGAGATTC+TGG 0.280245 4_2:-17744921 Msa0592390:CDS
AAAACTTTGTTTATCAAATA+GGG 0.285623 4_2:-17745500 Msa0592390:CDS
AACACTAGAGCGTTGGTTTC+CGG 0.290488 4_2:-17745614 Msa0592390:CDS
GCATTTCCAAAATAATATTC+TGG 0.302739 4_2:+17748358 None:intergenic
GATGTTTATGGTAATGATTT+TGG 0.308658 4_2:-17745040 Msa0592390:CDS
GATGTTTATGGTAATGATTT+TGG 0.308658 4_2:-17748116 Msa0592390:CDS
TTCTTGTGGATCAAATTGTT+TGG 0.335214 4_2:+17745354 None:intergenic
AGTGTCTCAGCCGTTGGATT+TGG 0.357834 4_2:+17746031 None:intergenic
CCAAAATAATATTCTGGTAA+TGG 0.371844 4_2:+17748364 None:intergenic
AATGAAAGTTGGGGAGTTGT+TGG 0.379006 4_2:-17745233 Msa0592390:CDS
TGGATTTGGTTAGCTTCAAC+TGG 0.379348 4_2:+17746045 None:intergenic
AAATGCAATTATAAGTTGTA+TGG 0.380673 4_2:-17745260 Msa0592390:CDS
GTGTTTGCCGGAATAGAAAA+AGG 0.382441 4_2:-17744962 Msa0592390:CDS
ACCTCTCACGTTGCATTAGT+TGG 0.383610 4_2:+17744859 None:intergenic
AAACCATGGAGAACTGCTAA+TGG 0.390568 4_2:+17745063 None:intergenic
GTATGGTTAAAATGAAAGTT+GGG 0.393088 4_2:-17745243 Msa0592390:CDS
GAAACCAACGCTCTAGTGTT+GGG 0.395003 4_2:+17745617 None:intergenic
TGTATGGTTAAAATGAAAGT+TGG 0.395906 4_2:-17745244 Msa0592390:CDS
CAAGAAATCCAAGCTTTGTT+GGG 0.403869 4_2:-17748193 Msa0592390:CDS
TATGGCATTTCATCAATTCA+TGG 0.407954 4_2:-17745684 Msa0592390:CDS
GTATTTAATAGATTCATGAT+AGG 0.414554 4_2:-17748412 None:intergenic
CTAGTTGACGGTATGTTCAT+TGG 0.417115 4_2:-17745745 Msa0592390:CDS
GCCAGCAAGAGGTTGGAAAA+AGG 0.417931 4_2:+17745990 None:intergenic
CTTTGTTGGGCTTGCTAACA+TGG 0.419451 4_2:-17748180 Msa0592390:CDS
GGACTTGTTACAGTGTTTGC+CGG 0.422586 4_2:-17744974 Msa0592390:CDS
GAGGTACATAGGTGGGTTCT+AGG 0.427095 4_2:+17745854 None:intergenic
TTTAACCATGTTGTCGTTGA+CGG 0.436978 4_2:-17745715 Msa0592390:CDS
AATAGATTCATGATAGGAGT+TGG 0.443370 4_2:-17748406 None:intergenic
AATACGATTTCCTTTCACTT+TGG 0.446701 4_2:-17745575 Msa0592390:CDS
GACACAACATTCATGAATTG+AGG 0.451357 4_2:+17744881 None:intergenic
GTTGGGGAGTTGTTGGAGGA+AGG 0.453345 4_2:-17745226 Msa0592390:CDS
AATGAAAGTTGGGGAGCTGT+TGG 0.454825 4_2:-17748309 Msa0592390:intron
TTTAACTCATCTTTGGTGCT+CGG 0.456890 4_2:-17745386 Msa0592390:CDS
CGTAGTGGATATAAAGCAAA+TGG 0.458289 4_2:-17744995 Msa0592390:CDS
CTATTAAATACTTCTTCTTG+TGG 0.458740 4_2:+17745340 None:intergenic
GTATTTAATAGAGTCGTGAT+AGG 0.460162 4_2:-17745328 Msa0592390:CDS
ACCTGCATTGTTGCATATAA+CGG 0.463614 4_2:+17745924 None:intergenic
TCAAGAAATCCAAGCTTTGT+TGG 0.466546 4_2:-17748194 Msa0592390:CDS
TGGTTGAAGCAAGTAGAAAT+CGG 0.475965 4_2:+17746094 None:intergenic
TGAAGAATCACTACGAGTCT+TGG 0.477110 4_2:-17745141 Msa0592390:CDS
TTAGCAAGCCCAACAAAGCT+TGG 0.481005 4_2:+17748185 None:intergenic
GGGGAGTTGTTGGAGGAAGG+TGG 0.485977 4_2:-17745223 Msa0592390:CDS
TATCCACTACGAACTGCTAC+AGG 0.486958 4_2:+17745007 None:intergenic
TATCCACTACGAACTGCTAC+AGG 0.486958 4_2:+17748083 None:intergenic
AATAGAGTCGTGATAGGAGT+TGG 0.491643 4_2:-17745322 Msa0592390:CDS
CATGTGGAAGACAAACTTGA+AGG 0.493205 4_2:+17744930 None:intergenic
AGGCACATCAAAACCATTGT+TGG 0.495948 4_2:-17745785 Msa0592390:CDS
GTTGGGGAGCTGTTGGAGGA+AGG 0.504563 4_2:-17748302 Msa0592390:CDS
ATTGGTTGTTGAACACCATC+CGG 0.506960 4_2:+17745595 None:intergenic
AAACTATTGATTTGACTCCA+TGG 0.507614 4_2:-17746122 Msa0592390:CDS
TGAAGAATCACTACGAGTCT+TGG 0.507673 4_2:-17748217 Msa0592390:CDS
GAAAGTTGGGGAGTTGTTGG+AGG 0.511101 4_2:-17745230 Msa0592390:CDS
GAAAGTTGGGGAGCTGTTGG+AGG 0.514885 4_2:-17748306 Msa0592390:intron
AACTATTGATTTGACTCCAT+GGG 0.516810 4_2:-17746121 Msa0592390:CDS
CAACAAAAGAATCACTCTGA+TGG 0.518700 4_2:-17745550 Msa0592390:CDS
CACATCAAAACCATTGTTGG+CGG 0.519877 4_2:-17745782 Msa0592390:CDS
TGCTGGCCGTCTCAAAATAA+CGG 0.522868 4_2:-17745974 Msa0592390:CDS
ATGAATTGATGAAATGCCAT+AGG 0.530467 4_2:+17745686 None:intergenic
ACATGAGATTCTGGAGGCAA+TGG 0.532230 4_2:-17744912 Msa0592390:CDS
AAAGTGAAAGGAAATCGTAT+TGG 0.533533 4_2:+17745577 None:intergenic
ATGGCATTTCATCAATTCAT+GGG 0.535218 4_2:-17745683 Msa0592390:CDS
ACAGAGAGTGTCTCAGCCGT+TGG 0.539317 4_2:+17746025 None:intergenic
GGAAACCAACGCTCTAGTGT+TGG 0.539690 4_2:+17745616 None:intergenic
TGAGACGGCCAGCAAGAGGT+TGG 0.540839 4_2:+17745983 None:intergenic
AAATAATATTCTGGTAATGG+TGG 0.544089 4_2:+17748367 None:intergenic
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4_2:-17745211 Msa0592390:CDS
GAGGAAGGTGGACTTTGTAA+AGG 0.546587 4_2:-17748287 Msa0592390:CDS
TCCGTTATATGCAACAATGC+AGG 0.551070 4_2:-17745925 Msa0592390:CDS
ATTGTTGGCGGTTCAAGTAA+CGG 0.553835 4_2:-17745770 Msa0592390:CDS
AACAAAAGAATCACTCTGAT+GGG 0.558688 4_2:-17745549 Msa0592390:CDS
AAACCATGGAGAACTGCTGA+TGG 0.560374 4_2:+17748139 None:intergenic
GGTAATGGTGGAACTAACCT+TGG 0.563399 4_2:+17745298 None:intergenic
ACACAACATTCATGAATTGA+GGG 0.567594 4_2:+17744882 None:intergenic
AAACCAACGCTCTAGTGTTG+GGG 0.573903 4_2:+17745618 None:intergenic
GTGATTCTTCAACTTCTCAT+CGG 0.575796 4_2:+17745153 None:intergenic
GTGATTCTTCAACTTCTCAT+CGG 0.575796 4_2:+17748229 None:intergenic
TTTGGTTAGCTTCAACTGGA+TGG 0.576856 4_2:+17746049 None:intergenic
AGTAGAAATCGGAGATCCCA+TGG 0.579335 4_2:+17746105 None:intergenic
TCTCTCCACTGATACAATCA+AGG 0.580251 4_2:-17746163 Msa0592390:CDS
GGGGAGCTGTTGGAGGAAGG+TGG 0.582104 4_2:-17748299 Msa0592390:CDS
TATCCATCAGCAGTTCTCCA+TGG 0.583070 4_2:-17748142 Msa0592390:CDS
TCGTTGACGGAAAGTCCCTA+TGG 0.585370 4_2:-17745702 Msa0592390:CDS
AATGGTGGAACTACTAACCT+TGG 0.585559 4_2:+17748382 None:intergenic
TGGTGCCTTGATTGTATCAG+TGG 0.587630 4_2:+17746158 None:intergenic
GTTACTTGAACCGCCAACAA+TGG 0.593750 4_2:+17745772 None:intergenic
TATCCATTAGCAGTTCTCCA+TGG 0.597915 4_2:-17745066 Msa0592390:CDS
TATGGTTAAAATGAAAGTTG+GGG 0.603920 4_2:-17745242 Msa0592390:CDS
TGAATTGATGAAATGCCATA+GGG 0.604499 4_2:+17745687 None:intergenic
AAGTATTCTCAGCTGCAGCA+TGG 0.612633 4_2:+17745890 None:intergenic
ACAATTAGAGGTACATAGGT+GGG 0.616014 4_2:+17745847 None:intergenic
CAAGTAACGGAACTAGTTGA+CGG 0.616630 4_2:-17745757 Msa0592390:CDS
ATCAGTGGAGAGAACTCGTA+CGG 0.619099 4_2:+17746173 None:intergenic
GACAATTAGAGGTACATAGG+TGG 0.624565 4_2:+17745846 None:intergenic
AACAAAGTTAGAAGCTACGA+AGG 0.627886 4_2:-17745805 Msa0592390:CDS
ACTTTCCGTCAACGACAACA+TGG 0.629179 4_2:+17745710 None:intergenic
CTAGAGCGTTGGTTTCCGGA+TGG 0.634274 4_2:-17745610 Msa0592390:CDS
AACCAACGCTCTAGTGTTGG+GGG 0.634809 4_2:+17745619 None:intergenic
ATCGACAATTAGAGGTACAT+AGG 0.637686 4_2:+17745843 None:intergenic
TCTTCCACATGAGATTCTGG+AGG 0.645425 4_2:-17744918 Msa0592390:CDS
TCATGATAGGAGTTGGACCA+AGG 0.646491 4_2:-17748399 Msa0592390:CDS
TTACCATAAACATCAAACCA+TGG 0.659822 4_2:+17745049 None:intergenic
TTACCATAAACATCAAACCA+TGG 0.659822 4_2:+17748125 None:intergenic
AAGCCTGTAGCAGTTCGTAG+TGG 0.668630 4_2:-17745010 Msa0592390:CDS
AAGCCTGTAGCAGTTCGTAG+TGG 0.668630 4_2:-17748086 Msa0592390:CDS
TCGTGATAGGAGTTGGACCA+AGG 0.676348 4_2:-17745315 Msa0592390:CDS
TTGAAGCTAACCAAATCCAA+CGG 0.678953 4_2:-17746041 Msa0592390:CDS
ATTGCCTCCAGAATCTCATG+TGG 0.679505 4_2:+17744914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATAAACATAAACT+GGG + chr4_2:17745627-17745646 None:intergenic 10.0%
!! TAAAAAAAATAAACATAAAC+TGG + chr4_2:17745628-17745647 None:intergenic 10.0%
!!! CAATTTTTAATTTTTATTCT+TGG + chr4_2:17746389-17746408 None:intergenic 10.0%
!!! ATAGTTTTTTTTTTTTTGAA+AGG - chr4_2:17746840-17746859 Msa0592390:intron 10.0%
!! AAAAAAATAAACATAAACTG+GGG + chr4_2:17745626-17745645 None:intergenic 15.0%
!!! CACTTTTAAGATATTTTTAT+TGG + chr4_2:17745904-17745923 None:intergenic 15.0%
!! AAATTAGCTAAGATAATTTA+CGG - chr4_2:17745967-17745986 Msa0592390:CDS 15.0%
!! AAAATTATTAGTGGTAATTA+CGG - chr4_2:17745998-17746017 Msa0592390:CDS 15.0%
!! GGTTTGATTAAAAAAAAAAA+TGG + chr4_2:17746219-17746238 None:intergenic 15.0%
!!! TTTAATTTTTATTCTTGGTT+TGG + chr4_2:17746384-17746403 None:intergenic 15.0%
!!! TATCAATTGATATCTTTTTT+TGG + chr4_2:17746815-17746834 None:intergenic 15.0%
!!! TATTTCTATTCTATTATTTG+AGG + chr4_2:17747056-17747075 None:intergenic 15.0%
!!! AAAAACTTTGTTTATCAAAT+AGG - chr4_2:17747770-17747789 Msa0592390:intron 15.0%
!!! AAAACTTTGTTTATCAAATA+GGG - chr4_2:17747771-17747790 Msa0592390:intron 15.0%
!! CAATTATAAGTTGTATTGTA+TGG - chr4_2:17744935-17744954 Msa0592390:CDS 20.0%
!!! CGATTTTCTAAAATTATTAG+CGG + chr4_2:17745398-17745417 None:intergenic 20.0%
!!! TTTTTTTCTTTCCTTATCTA+TGG - chr4_2:17745579-17745598 Msa0592390:CDS 20.0%
!! CTTATTATGAACATCAAATT+AGG - chr4_2:17745661-17745680 Msa0592390:CDS 20.0%
!! AATTAGCTAAGATAATTTAC+GGG - chr4_2:17745968-17745987 Msa0592390:CDS 20.0%
!! CTTGTAATTATGAGTTAATT+TGG + chr4_2:17746240-17746259 None:intergenic 20.0%
!!! TTCAGCTTTATTTCAATAAT+TGG - chr4_2:17746930-17746949 Msa0592390:intron 20.0%
!!! TCAGCTTTATTTCAATAATT+GGG - chr4_2:17746931-17746950 Msa0592390:intron 20.0%
!! AATATTGCTCAAGAAAAAAA+TGG - chr4_2:17747072-17747091 Msa0592390:intron 20.0%
!! ATATTGCTCAAGAAAAAAAT+GGG - chr4_2:17747073-17747092 Msa0592390:intron 20.0%
!! TTAAAAATCAATGAAGAGAT+CGG - chr4_2:17747832-17747851 Msa0592390:intron 20.0%
!! AAATGCAATTATAAGTTGTA+TGG - chr4_2:17744930-17744949 Msa0592390:CDS 20.0%
!!! TAAGAAATCCAAATTTTGTT+AGG - chr4_2:17748154-17748173 Msa0592390:CDS 20.0%
!!! AAATAATATTCTGGTAATGG+TGG + chr4_2:17744907-17744926 None:intergenic 25.0%
! CCAAAATAATATTCTGGTAA+TGG + chr4_2:17744910-17744929 None:intergenic 25.0%
!! CCATTACCAGAATATTATTT+TGG - chr4_2:17744907-17744926 Msa0592390:CDS 25.0%
! GCATTTCCAAAATAATATTC+TGG + chr4_2:17744916-17744935 None:intergenic 25.0%
! TGTATGGTTAAAATGAAAGT+TGG - chr4_2:17744951-17744970 Msa0592390:CDS 25.0%
! GTATGGTTAAAATGAAAGTT+GGG - chr4_2:17744952-17744971 Msa0592390:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG - chr4_2:17744953-17744972 Msa0592390:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4_2:17745155-17745174 Msa0592390:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4_2:17745159-17745178 Msa0592390:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4_2:17745160-17745179 Msa0592390:CDS 25.0%
!! TATACTACCTTTTTCTATTC+CGG + chr4_2:17745243-17745262 None:intergenic 25.0%
! TTCAGTGTAATTATATGTGT+CGG - chr4_2:17745423-17745442 Msa0592390:CDS 25.0%
! TTATGAACATCAAATTAGGT+CGG - chr4_2:17745665-17745684 Msa0592390:CDS 25.0%
!! GACATCATATATTGATTAGT+TGG + chr4_2:17745806-17745825 None:intergenic 25.0%
! GCTTATAAGCTAACTTATAA+TGG - chr4_2:17745942-17745961 Msa0592390:CDS 25.0%
!! GGATTTGGTAAAATTATTAG+TGG - chr4_2:17745989-17746008 Msa0592390:CDS 25.0%
! TGTTTGGTAAAATTAGTAGT+TGG - chr4_2:17746021-17746040 Msa0592390:CDS 25.0%
! AAGCTATAAGAATGAAACTT+TGG - chr4_2:17746191-17746210 Msa0592390:intron 25.0%
! TAAAAATTGTTACCGAAGAA+AGG - chr4_2:17746400-17746419 Msa0592390:intron 25.0%
! AAAAATTGTTACCGAAGAAA+GGG - chr4_2:17746401-17746420 Msa0592390:intron 25.0%
!! TATCATTTTATTGTCTCAGT+TGG - chr4_2:17746764-17746783 Msa0592390:intron 25.0%
!!! CAATTGATATCTTTTTTTGG+TGG + chr4_2:17746812-17746831 None:intergenic 25.0%
! TCTAGAAATTGTTATACACA+TGG - chr4_2:17746881-17746900 Msa0592390:intron 25.0%
!!! ATATTTATTTTGTTGCGAAG+TGG + chr4_2:17746989-17747008 None:intergenic 25.0%
!!! TATTTGAGGTTGATTTTTTG+AGG + chr4_2:17747042-17747061 None:intergenic 25.0%
!! ATAGTTTGATCACTTGATTT+TGG + chr4_2:17747136-17747155 None:intergenic 25.0%
!! ATAAAGAAGACCCTTTTTAT+TGG + chr4_2:17747200-17747219 None:intergenic 25.0%
! AAAAATGAATCGACAATTAG+AGG + chr4_2:17747439-17747458 None:intergenic 25.0%
!!! TTGTAGAGAAGATATTTTGA+TGG + chr4_2:17747860-17747879 None:intergenic 25.0%
! CTATTAAATACTTCTTCTTG+TGG + chr4_2:17747934-17747953 None:intergenic 25.0%
!!! AAATAATTTTCTGGTAATGG+TGG + chr4_2:17747988-17748007 None:intergenic 25.0%
!!! CCAAAATAATTTTCTGGTAA+TGG + chr4_2:17747991-17748010 None:intergenic 25.0%
!! CCATTACCAGAAAATTATTT+TGG - chr4_2:17747988-17748007 Msa0592390:intron 25.0%
!! GCATTTCCAAAATAATTTTC+TGG + chr4_2:17747997-17748016 None:intergenic 25.0%
! TGTATGGTTAAAATGAAAGT+TGG - chr4_2:17744951-17744970 Msa0592390:CDS 25.0%
! GTATGGTTAAAATGAAAGTT+GGG - chr4_2:17744952-17744971 Msa0592390:CDS 25.0%
! TATGGTTAAAATGAAAGTTG+GGG - chr4_2:17744953-17744972 Msa0592390:CDS 25.0%
!!! GATGTTTATGGTAATGATTT+TGG - chr4_2:17745155-17745174 Msa0592390:CDS 25.0%
!!! TTTATGGTAATGATTTTGGA+TGG - chr4_2:17745159-17745178 Msa0592390:CDS 25.0%
!!! TTATGGTAATGATTTTGGAT+GGG - chr4_2:17745160-17745179 Msa0592390:CDS 25.0%
!! TATACTACCTTTTTCTATTC+CGG + chr4_2:17745243-17745262 None:intergenic 25.0%
TTACCATAAACATCAAACCA+TGG + chr4_2:17745149-17745168 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4_2:17745161-17745180 Msa0592390:CDS 30.0%
!! CGTAGTGGATATAAAGTAAA+TGG - chr4_2:17745200-17745219 Msa0592390:CDS 30.0%
ACACAACATTCATGAATTGA+GGG + chr4_2:17745316-17745335 None:intergenic 30.0%
CTAAGATAATTTACGGGATT+TGG - chr4_2:17745974-17745993 Msa0592390:CDS 30.0%
!!! TGGATTTTTAGTAAGATGAC+AGG - chr4_2:17746041-17746060 Msa0592390:CDS 30.0%
!!! GGATTTTTAGTAAGATGACA+GGG - chr4_2:17746042-17746061 Msa0592390:CDS 30.0%
AATTTCTTCTTCCCTTTCTT+CGG + chr4_2:17746415-17746434 None:intergenic 30.0%
!!! TTGATATCTTTTTTTGGTGG+TGG + chr4_2:17746809-17746828 None:intergenic 30.0%
AATTGGGTGATTTCAATCAT+TGG - chr4_2:17746947-17746966 Msa0592390:intron 30.0%
ATTGGGTGATTTCAATCATT+GGG - chr4_2:17746948-17746967 Msa0592390:intron 30.0%
!! AAACTATTGATTTGACTCCA+TGG - chr4_2:17747149-17747168 Msa0592390:intron 30.0%
!! AACTATTGATTTGACTCCAT+GGG - chr4_2:17747150-17747169 Msa0592390:intron 30.0%
ACTTGCTTCAACCAATAAAA+AGG - chr4_2:17747186-17747205 Msa0592390:intron 30.0%
CTTGCTTCAACCAATAAAAA+GGG - chr4_2:17747187-17747206 Msa0592390:intron 30.0%
TGAATTGATGAAATGCCATA+GGG + chr4_2:17747587-17747606 None:intergenic 30.0%
ATGAATTGATGAAATGCCAT+AGG + chr4_2:17747588-17747607 None:intergenic 30.0%
TATGGCATTTCATCAATTCA+TGG - chr4_2:17747587-17747606 Msa0592390:intron 30.0%
ATGGCATTTCATCAATTCAT+GGG - chr4_2:17747588-17747607 Msa0592390:intron 30.0%
AAAGTGAAAGGAAATCGTAT+TGG + chr4_2:17747697-17747716 None:intergenic 30.0%
AATACGATTTCCTTTCACTT+TGG - chr4_2:17747696-17747715 Msa0592390:intron 30.0%
AACAAAAGAATCACTCTGAT+GGG - chr4_2:17747722-17747741 Msa0592390:intron 30.0%
TTCTTGTGGATCAAATTGTT+TGG + chr4_2:17747920-17747939 None:intergenic 30.0%
GTATTTAATAGAGTCGTGAT+AGG - chr4_2:17747943-17747962 Msa0592390:intron 30.0%
TTAGCAAGCCTAACAAAATT+TGG + chr4_2:17748165-17748184 None:intergenic 30.0%
TTACCATAAACATCAAACCA+TGG + chr4_2:17745149-17745168 None:intergenic 30.0%
!! TATGGTAATGATTTTGGATG+GGG - chr4_2:17745161-17745180 Msa0592390:CDS 30.0%
ACACAACATTCATGAATTGA+GGG + chr4_2:17745316-17745335 None:intergenic 30.0%
GTGATTCTTCAACTTCTCAT+CGG + chr4_2:17745045-17745064 None:intergenic 35.0%
! TCAAGAAATCCAAGCTTTGT+TGG - chr4_2:17745077-17745096 Msa0592390:CDS 35.0%
!! CAAGAAATCCAAGCTTTGTT+GGG - chr4_2:17745078-17745097 Msa0592390:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4_2:17745143-17745162 Msa0592390:CDS 35.0%
GACACAACATTCATGAATTG+AGG + chr4_2:17745317-17745336 None:intergenic 35.0%
CATAAATGTGCTTCATAGGT+TGG + chr4_2:17745339-17745358 None:intergenic 35.0%
CAACCTATGAAGCACATTTA+TGG - chr4_2:17745337-17745356 Msa0592390:CDS 35.0%
TGTCCATAAATGTGCTTCAT+AGG + chr4_2:17745343-17745362 None:intergenic 35.0%
AGTGTAATTATATGTGTCGG+TGG - chr4_2:17745426-17745445 Msa0592390:CDS 35.0%
CTTTCCTTATCTATGGACAA+TGG - chr4_2:17745586-17745605 Msa0592390:CDS 35.0%
CTTACCATTGTCCATAGATA+AGG + chr4_2:17745593-17745612 None:intergenic 35.0%
TGTTTGGAAACACTACAGTA+TGG - chr4_2:17745703-17745722 Msa0592390:CDS 35.0%
! ACAGTATGGTGATGAACATA+CGG - chr4_2:17745717-17745736 Msa0592390:CDS 35.0%
GCTTATAAGCTATTCGTCAT+AGG + chr4_2:17745932-17745951 None:intergenic 35.0%
TTAGTGGTAATTACGGTGTT+TGG - chr4_2:17746005-17746024 Msa0592390:CDS 35.0%
! AAGATTTGTTCGCTACTTTG+AGG - chr4_2:17746257-17746276 Msa0592390:intron 35.0%
AAGCTCTTGATCATAGCATA+AGG - chr4_2:17746294-17746313 Msa0592390:intron 35.0%
!! AGGAGTATGATCTTTTCAGT+TGG - chr4_2:17746477-17746496 Msa0592390:intron 35.0%
TGGTTGAAGCAAGTAGAAAT+CGG + chr4_2:17747180-17747199 None:intergenic 35.0%
TTGAAGCTAACCAAATCCAA+CGG - chr4_2:17747230-17747249 Msa0592390:intron 35.0%
!!! AGTGTTCCGTTATTTTGAGA+CGG + chr4_2:17747306-17747325 None:intergenic 35.0%
ACCTGCATTGTTGCATATAA+CGG + chr4_2:17747350-17747369 None:intergenic 35.0%
! ACAATTAGAGGTACATAGGT+GGG + chr4_2:17747427-17747446 None:intergenic 35.0%
! ATCGACAATTAGAGGTACAT+AGG + chr4_2:17747431-17747450 None:intergenic 35.0%
AACAAAGTTAGAAGCTACGA+AGG - chr4_2:17747466-17747485 Msa0592390:intron 35.0%
TTTAACCATGTTGTCGTTGA+CGG - chr4_2:17747556-17747575 Msa0592390:intron 35.0%
! TTTTGTTGCTCCAAAGTGAA+AGG + chr4_2:17747709-17747728 None:intergenic 35.0%
CAACAAAAGAATCACTCTGA+TGG - chr4_2:17747721-17747740 Msa0592390:intron 35.0%
!! AAGCGCTTTTAACTCATCTT+TGG - chr4_2:17747878-17747897 Msa0592390:intron 35.0%
!! TTTAACTCATCTTTGGTGCT+CGG - chr4_2:17747885-17747904 Msa0592390:intron 35.0%
GTGATTCTTCAACTTCTCAT+CGG + chr4_2:17745045-17745064 None:intergenic 35.0%
!!! TTTTGTTAGGCTTGCTAACA+TGG - chr4_2:17748167-17748186 Msa0592390:CDS 35.0%
TCTCCATGGTTTGATGTTTA+TGG - chr4_2:17745143-17745162 Msa0592390:CDS 35.0%
!! CGTAGTGGATATAAAGCAAA+TGG - chr4_2:17748276-17748295 Msa0592390:CDS 35.0%
GACACAACATTCATGAATTG+AGG + chr4_2:17745317-17745336 None:intergenic 35.0%
! AATGGTGGAACTACTAACCT+TGG + chr4_2:17744892-17744911 None:intergenic 40.0%
TGAAGAATCACTACGAGTCT+TGG - chr4_2:17745054-17745073 Msa0592390:CDS 40.0%
GGACTTATTACAGTGTTTGC+CGG - chr4_2:17745221-17745240 Msa0592390:CDS 40.0%
GTGTTTGCCGGAATAGAAAA+AGG - chr4_2:17745233-17745252 Msa0592390:CDS 40.0%
CATGTGGAAGACAAACTTGA+AGG + chr4_2:17745268-17745287 None:intergenic 40.0%
TTGTCTTCCACATGAGATTC+TGG - chr4_2:17745274-17745293 Msa0592390:CDS 40.0%
TGAAGCACATTTATGGACAC+TGG - chr4_2:17745344-17745363 Msa0592390:CDS 40.0%
TAGTTGGACACTATGAAGCA+TGG + chr4_2:17745495-17745514 None:intergenic 40.0%
TGAACATCAAATTAGGTCGG+CGG - chr4_2:17745668-17745687 Msa0592390:CDS 40.0%
GAAAACTGCTGGGTAAATAG+TGG - chr4_2:17746508-17746527 Msa0592390:intron 40.0%
TACGTGGAGATAAGGAAATC+AGG - chr4_2:17746603-17746622 Msa0592390:intron 40.0%
AATCAGGTGGAGATAACGAT+AGG - chr4_2:17746619-17746638 Msa0592390:intron 40.0%
TTGGTATAGACAATGTACCC+CGG - chr4_2:17746783-17746802 Msa0592390:intron 40.0%
!!! TATCTTTTTTTGGTGGTGGC+CGG + chr4_2:17746805-17746824 None:intergenic 40.0%
TCAATCATTGGGTGAATGTG+TGG - chr4_2:17746959-17746978 Msa0592390:intron 40.0%
TCTCTCCACTGATACAATCA+AGG - chr4_2:17747108-17747127 Msa0592390:intron 40.0%
!! TTTGGTTAGCTTCAACTGGA+TGG + chr4_2:17747225-17747244 None:intergenic 40.0%
!! TGGATTTGGTTAGCTTCAAC+TGG + chr4_2:17747229-17747248 None:intergenic 40.0%
TTGGAAAAAGGTAAGAGCAG+AGG + chr4_2:17747272-17747291 None:intergenic 40.0%
TCCGTTATATGCAACAATGC+AGG - chr4_2:17747346-17747365 Msa0592390:intron 40.0%
! GACAATTAGAGGTACATAGG+TGG + chr4_2:17747428-17747447 None:intergenic 40.0%
AGGCACATCAAAACCATTGT+TGG - chr4_2:17747486-17747505 Msa0592390:intron 40.0%
CACATCAAAACCATTGTTGG+CGG - chr4_2:17747489-17747508 Msa0592390:intron 40.0%
ATTGTTGGCGGTTCAAGTAA+CGG - chr4_2:17747501-17747520 Msa0592390:intron 40.0%
! CAAGTAACGGAACTAGTTGA+CGG - chr4_2:17747514-17747533 Msa0592390:intron 40.0%
CTAGTTGACGGTATGTTCAT+TGG - chr4_2:17747526-17747545 Msa0592390:intron 40.0%
ATTGGTTGTTGAACACCATC+CGG + chr4_2:17747679-17747698 None:intergenic 40.0%
AATAGAGTCGTGATAGGAGT+TGG - chr4_2:17747949-17747968 Msa0592390:intron 40.0%
AATGAAAGTTGGGGAGTTGT+TGG - chr4_2:17748038-17748057 Msa0592390:intron 40.0%
TGAAGAATCACTACGAGTCT+TGG - chr4_2:17745054-17745073 Msa0592390:CDS 40.0%
TATCCATTAGCAGTTCTCCA+TGG - chr4_2:17748205-17748224 Msa0592390:CDS 40.0%
AAACCATGGAGAACTGCTAA+TGG + chr4_2:17748211-17748230 None:intergenic 40.0%
GTGTTTGCCGGAATAGAAAA+AGG - chr4_2:17745233-17745252 Msa0592390:CDS 40.0%
CATGTGGAAGACAAACTTGA+AGG + chr4_2:17745268-17745287 None:intergenic 40.0%
TTGTCTTCCACATGAGATTC+TGG - chr4_2:17745274-17745293 Msa0592390:CDS 40.0%
AATGAAAGTTGGGGAGCTGT+TGG - chr4_2:17744962-17744981 Msa0592390:CDS 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG - chr4_2:17744984-17745003 Msa0592390:CDS 45.0%
TTAGCAAGCCCAACAAAGCT+TGG + chr4_2:17745089-17745108 None:intergenic 45.0%
!! CTTTGTTGGGCTTGCTAACA+TGG - chr4_2:17745091-17745110 Msa0592390:CDS 45.0%
TATCCATCAGCAGTTCTCCA+TGG - chr4_2:17745129-17745148 Msa0592390:CDS 45.0%
AAACCATGGAGAACTGCTGA+TGG + chr4_2:17745135-17745154 None:intergenic 45.0%
TATCCACTACGAACTGCTAC+AGG + chr4_2:17745191-17745210 None:intergenic 45.0%
TCTTCCACATGAGATTCTGG+AGG - chr4_2:17745277-17745296 Msa0592390:CDS 45.0%
ATTGCCTCCAGAATCTCATG+TGG + chr4_2:17745284-17745303 None:intergenic 45.0%
! ACATGAGATTCTGGAGGCAA+TGG - chr4_2:17745283-17745302 Msa0592390:CDS 45.0%
TATGAAGCATGGACACTCCT+CGG + chr4_2:17745484-17745503 None:intergenic 45.0%
! GTATGGTGATGAACATACGG+TGG - chr4_2:17745720-17745739 Msa0592390:CDS 45.0%
ACATACGGTGGTCTAACTCT+TGG - chr4_2:17745732-17745751 Msa0592390:CDS 45.0%
CATACGGTGGTCTAACTCTT+GGG - chr4_2:17745733-17745752 Msa0592390:CDS 45.0%
TAGCGTAAGTTAACTCACGC+AGG - chr4_2:17746457-17746476 Msa0592390:intron 45.0%
TGGAAATGAGCGAAAACTGC+TGG - chr4_2:17746497-17746516 Msa0592390:intron 45.0%
GGAAATGAGCGAAAACTGCT+GGG - chr4_2:17746498-17746517 Msa0592390:intron 45.0%
AAACAACATGCGACTGTACG+TGG - chr4_2:17746587-17746606 Msa0592390:intron 45.0%
GTGGAGATAAGGAAATCAGG+TGG - chr4_2:17746606-17746625 Msa0592390:intron 45.0%
!!! ATCTTTTTTTGGTGGTGGCC+GGG + chr4_2:17746804-17746823 None:intergenic 45.0%
ATCAGTGGAGAGAACTCGTA+CGG + chr4_2:17747101-17747120 None:intergenic 45.0%
TGGTGCCTTGATTGTATCAG+TGG + chr4_2:17747116-17747135 None:intergenic 45.0%
AGTAGAAATCGGAGATCCCA+TGG + chr4_2:17747169-17747188 None:intergenic 45.0%
! ACCTTTTTCCAACCTCTTGC+TGG - chr4_2:17747280-17747299 Msa0592390:intron 45.0%
TGCTGGCCGTCTCAAAATAA+CGG - chr4_2:17747297-17747316 Msa0592390:intron 45.0%
AAGTATTCTCAGCTGCAGCA+TGG + chr4_2:17747384-17747403 None:intergenic 45.0%
GTTACTTGAACCGCCAACAA+TGG + chr4_2:17747502-17747521 None:intergenic 45.0%
ACTTTCCGTCAACGACAACA+TGG + chr4_2:17747564-17747583 None:intergenic 45.0%
AAACCAACGCTCTAGTGTTG+GGG + chr4_2:17747656-17747675 None:intergenic 45.0%
GAAACCAACGCTCTAGTGTT+GGG + chr4_2:17747657-17747676 None:intergenic 45.0%
!! AACACTAGAGCGTTGGTTTC+CGG - chr4_2:17747657-17747676 Msa0592390:intron 45.0%
! GGTAATGGTGGAACTAACCT+TGG + chr4_2:17747976-17747995 None:intergenic 45.0%
! GAGGAAGGTGGACTTTGTAA+AGG - chr4_2:17744984-17745003 Msa0592390:CDS 45.0%
TATCCACTACGAACTGCTAC+AGG + chr4_2:17745191-17745210 None:intergenic 45.0%
!! GGACTTGTTACAGTGTTTGC+CGG - chr4_2:17748297-17748316 Msa0592390:CDS 45.0%
TCTTCCACATGAGATTCTGG+AGG - chr4_2:17745277-17745296 Msa0592390:CDS 45.0%
ATTGCCTCCAGAATCTCATG+TGG + chr4_2:17745284-17745303 None:intergenic 45.0%
! ACATGAGATTCTGGAGGCAA+TGG - chr4_2:17745283-17745302 Msa0592390:CDS 45.0%
!! TAAAATAATAATAATAATAG+TGG - chr4_2:17745874-17745893 Msa0592390:CDS 5.0%
AAGCCTGTAGCAGTTCGTAG+TGG - chr4_2:17745185-17745204 Msa0592390:CDS 50.0%
TATGGACACTGGACACGACA+CGG - chr4_2:17745355-17745374 Msa0592390:CDS 50.0%
GATGAACCGTATTCCGGTGT+CGG + chr4_2:17745462-17745481 None:intergenic 50.0%
TCCTCGGATGAACCGTATTC+CGG + chr4_2:17745468-17745487 None:intergenic 50.0%
GTCCAACTATGCAACGTGAG+AGG - chr4_2:17745506-17745525 Msa0592390:CDS 50.0%
CACCTCTCACGTTGCATAGT+TGG + chr4_2:17745511-17745530 None:intergenic 50.0%
GCGGCGTCTAACATCATGTT+TGG - chr4_2:17745687-17745706 Msa0592390:CDS 50.0%
TGCGACTGTACGTGGAGATA+AGG - chr4_2:17746595-17746614 Msa0592390:intron 50.0%
!!! TCTTTTTTTGGTGGTGGCCG+GGG + chr4_2:17746803-17746822 None:intergenic 50.0%
AGTGTCTCAGCCGTTGGATT+TGG + chr4_2:17747243-17747262 None:intergenic 50.0%
GCCAGCAAGAGGTTGGAAAA+AGG + chr4_2:17747284-17747303 None:intergenic 50.0%
!!! ATTTTGAGACGGCCAGCAAG+AGG + chr4_2:17747295-17747314 None:intergenic 50.0%
! GAGGTACATAGGTGGGTTCT+AGG + chr4_2:17747420-17747439 None:intergenic 50.0%
TCGTTGACGGAAAGTCCCTA+TGG - chr4_2:17747569-17747588 Msa0592390:intron 50.0%
! AACCAACGCTCTAGTGTTGG+GGG + chr4_2:17747655-17747674 None:intergenic 50.0%
GGAAACCAACGCTCTAGTGT+TGG + chr4_2:17747658-17747677 None:intergenic 50.0%
TCGTGATAGGAGTTGGACCA+AGG - chr4_2:17747956-17747975 Msa0592390:intron 50.0%
GAAAGTTGGGGAGTTGTTGG+AGG - chr4_2:17748041-17748060 Msa0592390:intron 50.0%
AAGCCTGTAGCAGTTCGTAG+TGG - chr4_2:17745185-17745204 Msa0592390:CDS 50.0%
GAAAGTTGGGGAGCTGTTGG+AGG - chr4_2:17744965-17744984 Msa0592390:CDS 55.0%
TCGTGTCCGACACCGGAATA+CGG - chr4_2:17745453-17745472 Msa0592390:CDS 55.0%
ACCGGAATACGGTTCATCCG+AGG - chr4_2:17745464-17745483 Msa0592390:CDS 55.0%
!! ACAGAGAGTGTCTCAGCCGT+TGG + chr4_2:17747249-17747268 None:intergenic 55.0%
AACCCCCAACACTAGAGCGT+TGG - chr4_2:17747650-17747669 Msa0592390:intron 55.0%
!! CTAGAGCGTTGGTTTCCGGA+TGG - chr4_2:17747661-17747680 Msa0592390:intron 55.0%
GTTGGGGAGTTGTTGGAGGA+AGG - chr4_2:17748045-17748064 Msa0592390:intron 55.0%
GTTGGGGAGCTGTTGGAGGA+AGG - chr4_2:17744969-17744988 Msa0592390:CDS 60.0%
TGAGACGGCCAGCAAGAGGT+TGG + chr4_2:17747291-17747310 None:intergenic 60.0%
GGGGAGTTGTTGGAGGAAGG+TGG - chr4_2:17748048-17748067 Msa0592390:intron 60.0%
GGGGAGCTGTTGGAGGAAGG+TGG - chr4_2:17744972-17744991 Msa0592390:CDS 65.0%
TGGCGTGTCGTGTCCGACAC+CGG - chr4_2:17745446-17745465 Msa0592390:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 17744874 17748419 17744874 ID=Msa0592390;Name=Msa0592390
chr4_2 mRNA 17744874 17748419 17744874 ID=Msa0592390-mRNA-1;Parent=Msa0592390;Name=Msa0592390-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|0|0|3|0|549
chr4_2 exon 17748350 17748419 17748350 ID=Msa0592390-mRNA-1:exon:5110;Parent=Msa0592390-mRNA-1
chr4_2 exon 17748079 17748323 17748079 ID=Msa0592390-mRNA-1:exon:5109;Parent=Msa0592390-mRNA-1
chr4_2 exon 17744874 17746208 17744874 ID=Msa0592390-mRNA-1:exon:5108;Parent=Msa0592390-mRNA-1
chr4_2 CDS 17748350 17748419 17748350 ID=Msa0592390-mRNA-1:cds;Parent=Msa0592390-mRNA-1
chr4_2 CDS 17748079 17748323 17748079 ID=Msa0592390-mRNA-1:cds;Parent=Msa0592390-mRNA-1
chr4_2 CDS 17744874 17746208 17744874 ID=Msa0592390-mRNA-1:cds;Parent=Msa0592390-mRNA-1
Gene Sequence

>Msa0592390

ATGATAGGAGTTGGACCAAGGTTAGTAGTTCCACCATTACCAGAATATTATTTTGGAAATGCAATTATAATTGGGGAGCTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGCGAGATGAACAAGTTAATTGCTTCGCACACCGATGAGAAGTTGAAGAATCACTACGAGTCTTGGTCAAGAAATCCAAGCTTTGTTGGGCTTGCTAACATGGTAAAATATAATTTCATATCCATCAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTAGCAGTTCGTAGTGGATATAAAAAAAAAATGGGAACCGTACGAGTTCTCTCCACTGATACAATCAAGGCACCAAAATCAAGTGATCAAACTATTGATTTGACTCCATGGGATCTCCGATTTCTACTTGCTTCAACCAATAAAAAGGGTCTTCTTTATCACCATCCAGTTGAAGCTAACCAAATCCAACGGCTGAGACACTCTCTGTCCTCTGCTCTTACCTTTTTCCAACCTCTTGCTGGCCGTCTCAAAATAACGGAACACTCAGACAACACTGTCTCGTGTTCCGTTATATGCAACAATGCAGGTGTACTCTTTATCCATGCTGCAGCTGAGAATACTTGTGTTGCTGACATCCTAGAACCCACCTATGTACCTCTAATTGTCGATTCATTTTTTGTTTTAAACAAAGTTAGAAGCTACGAAGGCACATCAAAACCATTGTTGGCGGTTCAAGTAACGGAACTAGTTGACGGTATGTTCATTGGTTGCTCATTTAACCATGTTGTCGTTGACGGAAAGTCCCTATGGCATTTCATCAATTCATGGGCTGAAATTTCACGTAGTTGTTGTCATCATCAAATATCTAAACCCCCAACACTAGAGCGTTGGTTTCCGGATGGTGTTCAACAACCAATACGATTTCCTTTCACTTTGGAGCAACAAAAGAATCACTCTGATGGGTTGAGTTTTTCGTCGTTTGATGATGAAAAACTTTGTTTATCAAATAGGGTATTTCATTTCACAAAAGAGAAAATTGTGCAGCTGAAATTAAAAATCAATGAAGAGATCGGTACCATCAAAATATCTTCTCTACAAGCGCTTTTAACTCATCTTTGGTGCTCGGTGATGCGTTCCAAACAATTTGATCCACAAGAAGAAGTATTTAATAGAGTCGTGATAGGAGTTGGACCAAGGTTAGTTCCACCATTACCAGAAAATTATTTTGGAAATGCAATTATAAGTTGTATGGTTAAAATGAAAGTTGGGGAGTTGTTGGAGGAAGGTGGACTTTGTAAAGGTGCTTGCGAGATGAACAAGTTAATTGCTTCACACACCGATGAGAAGTTGAAGAATCACTACGAGTCTTGGTTAAGAAATCCAAATTTTGTTAGGCTTGCTAACATGGTAAAAAATAATTTCATATCCATTAGCAGTTCTCCATGGTTTGATGTTTATGGTAATGATTTTGGATGGGGAAAGCCTGTAGCAGTTCGTAGTGGATATAAAGCAAATGGACTTGTTACAGTGTTTGCCGGAATAGAAAAAGGTAGTATAGACCTTCAAGTTTGTCTTCCACATGAGATTCTGGAGGCAATGGAAAATGACCCTCAATTCATGAATGTTGTGTCCAACTAA
Protein sequence

>Msa0592390

MIGVGPRLVVPPLPEYYFGNAIIIGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWSRNPSFVGLANMVKYNFISISSSPWFDVYGNDFGWGKPVAVRSGYKKKMGTVRVLSTDTIKAPKSSDQTIDLTPWDLRFLLASTNKKGLLYHHPVEANQIQRLRHSLSSALTFFQPLAGRLKITEHSDNTVSCSVICNNAGVLFIHAAAENTCVADILEPTYVPLIVDSFFVLNKVRSYEGTSKPLLAVQVTELVDGMFIGCSFNHVVVDGKSLWHFINSWAEISRSCCHHQISKPPTLERWFPDGVQQPIRFPFTLEQQKNHSDGLSFSSFDDEKLCLSNRVFHFTKEKIVQLKLKINEEIGTIKISSLQALLTHLWCSVMRSKQFDPQEEVFNRVVIGVGPRLVPPLPENYFGNAIISCMVKMKVGELLEEGGLCKGACEMNKLIASHTDEKLKNHYESWLRNPNFVRLANMVKNNFISISSSPWFDVYGNDFGWGKPVAVRSGYKANGLVTVFAGIEKGSIDLQVCLPHEILEAMENDPQFMNVVSN*